Metabolomics study of the metabolic changes in hepatoblastoma cells in response to NTCP/SLC10A1 overexpression.
In this study, we found that NTCP (SLC10A1) expression was downregulated in HB cells and tissues, and it was demonstrated that NTCP (SLC10A1) reduced cell viability, promoted cell cycle arrest and induced apoptosis of HB cells. The metabolic profiles of HB cells with NTCP (SLC10A1) overexpression were further examined to determine their biochemical alterations and deepen our understanding on the metabolic regulation of NTCP (SLC10A1) overexpression. The metabolomics study based on ultra performance liquid chromatography-mass spectrometry revealed alterations in the metabolites of HB cells following NTCP (SLC10A1) overexpre...
Source: The International Journal of Biochemistry and Cell Biology - May 22, 2020 Category: Biochemistry Authors: Wang J, Tian R, Shan Y, Li J, Gao H, Xie C, Ma Y, Wu Y, Ji B, Gu S, Xu M Tags: Int J Biochem Cell Biol Source Type: research

The diagnostic, prognostic and therapeutic potential of circulating microRNAs in ovarian cancer.
Abstract Ovarian cancer (OC) is often diagnosed at an advanced stage because of the late onset of symptoms, and this together with the lack of effective treatments, has meant it is associated with a very high mortality. The aberrant expression of MicroRNA (miRNA) contributes to the initiation and development of human tumors including OC. Several miRNAs are secreted by tumor cells and can be identified in body fluids. Serum miRNAs levels are associated with several clinical conditions, and may be used to predict prognosis and response to treatments in some cancers including OC. This review summarizes the current pr...
Source: The International Journal of Biochemistry and Cell Biology - May 16, 2020 Category: Biochemistry Authors: Aboutalebi H, Bahrami A, Soleimani A, Saeedi N, Rahmani F, Khazaei M, Fiuji H, Shafiee M, Ferns GA, Avan A, Hassanian SM Tags: Int J Biochem Cell Biol Source Type: research

Carbohydrate Responsive Element Binding Protein (ChREBP) negatively regulates osteoblast differentiation via protein phosphatase 2A C α dependent manner.
Carbohydrate Responsive Element Binding Protein (ChREBP) negatively regulates osteoblast differentiation via protein phosphatase 2A Cα dependent manner. Int J Biochem Cell Biol. 2020 May 13;:105766 Authors: Kim KM, Kim EJ, Jang WG Abstract Carbohydrate responsive element binding protein (ChREBP) is a major transcription factor of lipogenesis regulated by glucose status in the liver. However, the function of ChREBP in osteogenic differentiation is unclear. The present study examined the role of ChREBP in osteoblast differentiation in MC3T3-E1 preosteoblast cell line. The mRNA expression of ChREBP...
Source: The International Journal of Biochemistry and Cell Biology - May 13, 2020 Category: Biochemistry Authors: Kim KM, Kim EJ, Jang WG Tags: Int J Biochem Cell Biol Source Type: research

An introduction to the methodology of expansion microscopy.
Abstract Expansion microscopy is a novel, fluorescence imaging technique, which allows three-dimensional nanoscale imaging of specimens on a conventional fluorescence microscope. This is achieved through an innovative sample treatment, which culminates in approximately 4.5-fold expansion of specimens in each dimension. This allows 70 nm lateral and 200 nm axial resolution. To further develop application of the technique, there has been considerable focus on improving the methodology by i) extending the efficacy of labelling, ii) enabling multi-colour labelling of different biomolecules simultaneously...
Source: The International Journal of Biochemistry and Cell Biology - May 11, 2020 Category: Biochemistry Authors: Faulkner EL, Thomas SG, Neely RK Tags: Int J Biochem Cell Biol Source Type: research

Mycobacterium smegmatis MSMEG_0129 is a nutrition-associated regulator that interacts with CarD and ClpP2.
Abstract Mycobacterium smegmatis MSMEG_0129 and Rv0164, its homologue in Mycobacterium tuberculosis, are single START-domain proteins essential for bacterial growth and survival, but their biochemical activities and biological roles remain undetermined. Here, we probed the possible functions of MSMEG_0129 and its underlying mechanisms by determining its cellular location, searching for its interaction partners and monitoring its transcription profile. MSMEG_0129, and Rv0164 by extension, were found to be cytosolic proteins rather than secreted components as previously understood. Increases in MSMEG_0129 expression...
Source: The International Journal of Biochemistry and Cell Biology - May 7, 2020 Category: Biochemistry Authors: Zhou Y, Wei W, Fleming J, Ye C, Zheng S, Liu F, Zhou L, Bi L, Liu W Tags: Int J Biochem Cell Biol Source Type: research

Calcium-activated potassium channels: implications for aging and age-related neurodegeneration.
Abstract Population aging, as well as the handling of age-associated diseases, is a worldwide increasing concern. Among them, Alzheimer's disease stands out as the major cause of dementia culminating in full dependence on other people for basic functions. However, despite numerous efforts, in the last decades, there was no new approved therapeutic drug for the treatment of the disease. Calcium-activated potassium channels have emerged as a potential tool for neuronal protection by modulating intracellular calcium signaling. Their subcellular localization is determinant of their functional effects. When located on ...
Source: The International Journal of Biochemistry and Cell Biology - April 27, 2020 Category: Biochemistry Authors: Trombetta-Lima M, Krabbendam IE, Dolga AM Tags: Int J Biochem Cell Biol Source Type: research

Tackling mitochondrial diversity in brain function: from animal models to human brain organoids.
Abstract Mitochondria exhibit high degree of heterogeneity within various tissues, including differences in terms of morphology, quantity, or function. Mitochondria can even vary among distinct sub-compartments of the same cell. Emerging evidence suggest that the molecular diversity of mitochondria can influence the identity and functionality of a given cell type. Human pathologies affecting mitochondria typically cause tissue and cell-type-specific impairment. Mitochondrial diversity could thus play a contributing role not only in physiological cell fate specification but also during pathological disease developm...
Source: The International Journal of Biochemistry and Cell Biology - April 24, 2020 Category: Biochemistry Authors: Menacho C, Prigione A Tags: Int J Biochem Cell Biol Source Type: research

Overexpression of LINC00691 promotes the proliferation and invasion of gastric cancer cells via the Janus kinase/signal transducer and activator of transcription signalling pathway.
This report aims to explore how LINC00691 regulates the proliferation and invasion of gastric cancer (GC). Clinical tissue and serum samples, as well as specimens in the Cancer Genome Atlas (TCGA) database, were used to analyse the expression of LINC00691 in GC. Our data indicated that the expression of LINC00691 in GC was significantly higher than that in healthy controls and was associated with clinicopathological features and survival time. In the GC cell lines MKN-45 and HGC-27, the knockdown of LINC00691 suppressed proliferation, colony formation, migration, and invasion. Bioinformatics analysis and luciferase reporte...
Source: The International Journal of Biochemistry and Cell Biology - April 21, 2020 Category: Biochemistry Authors: Liang W, Xia B, He C, Zhai G, Li M, Zhou J Tags: Int J Biochem Cell Biol Source Type: research

Analysis methods for interrogating spatial organisation of single molecule localisation microscopy data.
Abstract Single-molecule localisation microscopy (SMLM) gives access to biological information below the diffraction limit, allowing nanoscale cellular structures to be probed. The data output is unlike that of conventional microscopy images, instead consisting of an array of molecular coordinates. These represent a spatial point pattern that attempts to approximate, as closely as possible, the underlying positions of the molecules of interest. Here, we review the analysis methods that can be used to extract biological insight from SMLM data, in particular for the application of quantifying nanoscale molecular clu...
Source: The International Journal of Biochemistry and Cell Biology - April 20, 2020 Category: Biochemistry Authors: Nieves DJ, Owen DM Tags: Int J Biochem Cell Biol Source Type: research

Cell adhesion molecule 4 suppresses cell growth and metastasis by inhibiting the Akt signaling pathway in non-small cell lung cancer.
We examined CADM4 levels in NSCLC tissues using real-time PCR and western blot. A549 and NCI-H1299 cells were then transfected with pcDNA3.1-CADM4 plasmid or siCADM4 to overexpress or knock down CADM4. Cell proliferation, cell cycle distribution, migration, and invasion were evaluated. NSCLC cells transfected with pcDNA3.1-CADM4 plasmid or siCADM4 were treated with SC79 or LY294002, respectively, to investigate the involvement of the Akt signaling pathway. Male nude mice were subcutaneously injected with stably transfected cells (1 × 106 cells/mice) to observe tumor growth. Stably transfectants were inj...
Source: The International Journal of Biochemistry and Cell Biology - April 20, 2020 Category: Biochemistry Authors: Luo F, Zhao Y, Liu J Tags: Int J Biochem Cell Biol Source Type: research

Curcumin circumvent Lactate-induced Chemoresistance in Hepatic cancer cells through modulation of Hydroxycarboxylic acid receptor-1.
Abstract Curcumin has been demonstrated to affect the chemoresistance in cancer cells of various origins. However, its ability to modulate lactate-induced chemoresistance remains unclear. The Present investigation demonstrates that curcumin inhibits the survival of HepG2 and HuT78 cells and can modulate chemo-susceptibility of HepG2 cells.Experimental simulation of simultaneous and pre-treatment suggest cooperatively between curcumin and anticancer drugs as well as the modulation of molecular regulators. Inhibition of glucose consumption, lactate production, extracellular acidity and augmented level of Nitric oxid...
Source: The International Journal of Biochemistry and Cell Biology - April 20, 2020 Category: Biochemistry Authors: Soni VK, Shukla D, Kumar A, Vishvakarma NK Tags: Int J Biochem Cell Biol Source Type: research

The pivotal role of Ubiquitin-activating enzyme E1 (UBA1) in neuronal health and neurodegeneration.
Abstract Ubiquitin-activating enzyme E1, UBA1, functions at the apex of the enzymatic ubiquitylation cascade, catalysing ubiquitin activation. UBA1 is thus of fundamental importance to the modulation of ubiquitin homeostasis and to all downstream ubiquitylation-dependent cellular processes, including proteolysis through the ubiquitin-proteasome system and selective autophagy. The proteasome-dependent and -independent functions of UBA1 contribute significantly to a range of processes crucial to neuronal health. The significance of UBA1 activity to neuronal health is clear in light of accumulating evidence implicati...
Source: The International Journal of Biochemistry and Cell Biology - April 18, 2020 Category: Biochemistry Authors: Lambert-Smith IA, Saunders DN, Yerbury JJ Tags: Int J Biochem Cell Biol Source Type: research

Circular RNA expression profile of Alzheimer's disease and its clinical significance as biomarkers for the disease risk and progression.
CONCLUSION: Our study provides an overview of circRNA expression profile in AD, and identifies that circ-AXL, circ-GPHN and circ-PCCA hold clinical implications for guiding disease management in AD patients. PMID: 32315771 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - April 18, 2020 Category: Biochemistry Authors: Li Y, Fan H, Sun J, Ni M, Zhang L, Chen C, Hong X, Fang F, Zhang W, Ma P Tags: Int J Biochem Cell Biol Source Type: research

Inclusion of temporal information in single cell transcriptomics.
Abstract Single cell transcriptomics has emerged as a powerful method for dissecting cell type diversity and for understanding mechanisms of cell fate decisions. However, inclusion of temporal information remains challenging, since each cell can be measured only once by sequencing analysis. Here, we discuss recent progress and current efforts towards inclusion of temporal information in single cell transcriptomics. Even from snapshot data, temporal dynamics can be computationally inferred via pseudo-temporal ordering of single cell transcriptomes. Temporal information can also come from analysis of intronic reads ...
Source: The International Journal of Biochemistry and Cell Biology - April 10, 2020 Category: Biochemistry Authors: Olivares-Chauvet P, Junker JP Tags: Int J Biochem Cell Biol Source Type: research

Corrigendum to "Transcriptome sequencing reveals fibrotic associated-genes involved in bovine mammary fibroblasts with Staphylococcus aureus" [Int. J. Biochem. Cell Biol. 121 (April) (2020) 105696].
Corrigendum to "Transcriptome sequencing reveals fibrotic associated-genes involved in bovine mammary fibroblasts with Staphylococcus aureus" [Int. J. Biochem. Cell Biol. 121 (April) (2020) 105696]. Int J Biochem Cell Biol. 2020 Mar 30;:105743 Authors: Miao Z, Ding Y, Zhao N, Chen X, Cheng H, Wang J, Liu Y, Wang F PMID: 32241594 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - March 30, 2020 Category: Biochemistry Authors: Miao Z, Ding Y, Zhao N, Chen X, Cheng H, Wang J, Liu Y, Wang F Tags: Int J Biochem Cell Biol Source Type: research

Crystal structure of human archease, a key cofactor of tRNA splicing ligase complex.
Abstract The human archease, hereafter named HArch, is identified as a key cofactor of the tRNA-splicing ligase complex, and a potential therapeutic target for treating nervous system injuries. However, little is known about the structural basis of HArch in tRNA maturation, mRNA splicing, and RNA repair. Here we report the crystal structures of HArch and its two mutants D51A and D178A with resolutions ranging from 1.96 Å to 3.4 Å. HArch is composed of an extended N-terminal protrusion domain (NTD) and one compacted C-terminal domain (CTD). Unlike previously reported homologous proteins, t...
Source: The International Journal of Biochemistry and Cell Biology - March 29, 2020 Category: Biochemistry Authors: Duan S, Gao W, Chen Z, Li Z, Li S, Gan J, Chen X, Li J Tags: Int J Biochem Cell Biol Source Type: research

The Long Noncoding RNA Ptprd-IR is a Novel Molecular Target for TGF- β1-Mediated Nephritis.
The Long Noncoding RNA Ptprd-IR is a Novel Molecular Target for TGF-β1-Mediated Nephritis. Int J Biochem Cell Biol. 2020 Mar 12;:105742 Authors: Pu Y, Zhao H, Wu X, Mei M, Shen B Abstract The role of microRNAs (miRNAs) in chronic kidney disease (CKD) is relatively well established, but much less is known about the role(s) of long noncoding RNAs (lncRNAs). Transforming growth factor β1 (TGF-β1) mediates inflammatory and fibrogenic signaling in CKD via the transcription factor Smad3; however, the extent of lncRNAs-based regulation of TGF-β1 signaling in CKD remains unknown. Herein, w...
Source: The International Journal of Biochemistry and Cell Biology - March 12, 2020 Category: Biochemistry Authors: Pu Y, Zhao H, Wu X, Mei M, Shen B Tags: Int J Biochem Cell Biol Source Type: research

Long non-coding RNA CCAT1 is overexpressed in endometrial cancer and regulates growth and transcriptome of endometrial adenocarcinoma cells.
In this study, to elucidate the role of this lncRNA in endometrial cancer, we examined its expression in normal endometrium and type 1 endometrial cancer and knocked down its expression in endometrial cancer cell lines followed by transcriptome and pathway analyses. METHODS: CCAT1 expression was examined in 100 tissue samples of normal endometrium and type 1 endometrial cancer tissues by means of RT-qPCR. Knockdown of CCAT1 expression in HEC-1B and RL95/2 endometrial cancer cells was performed by siRNA transfection. Affymetrix GeneChip arrays were used to elucidate the effect of both lncRNAs on the transcriptome of th...
Source: The International Journal of Biochemistry and Cell Biology - March 12, 2020 Category: Biochemistry Authors: Treeck O, Skrzypczak M, Schüler-Toprak S, Weber F, Ortmann O Tags: Int J Biochem Cell Biol Source Type: research

AKAP5 anchors PKA to enhance regulation of the HERG channel.
Abstract The activation of the β-adrenergic receptor (β-AR) regulates the human ether a-go-go-related gene (HERG) channel via protein kinase A (PKA), which in turn induces lethal arrhythmia in patients with long QT syndromes (LQTS). However, the role of A-kinase anchoring proteins (AKAPs) in PKA's regulation of the HERG channel and its molecular mechanism are not clear. Here, HEK293 cells were transfected with the HERG gene alone or co-transfected with HERG and AKAP5 using Lipofectamine 2000. Western blotting was performed to determine HERG protein expression, and immunofluorescence and immunoprecipitati...
Source: The International Journal of Biochemistry and Cell Biology - March 12, 2020 Category: Biochemistry Authors: Huang T, Zhang B, Wang Z, Wang Y, Li W, Wang H Tags: Int J Biochem Cell Biol Source Type: research

Activation of PPAR γ intensified the effect of arsenic trioxide in acute promyelocytic leukemia through the suppression of PI3K/Akt pathway: proposing a novel anticancer effect for pioglitazone.
In conclusion, given the significant efficacy as well as the safety profile of pioglitazone in potentiating the anticancer effect of chemotherapeutic drugs, the present study suggests it as a promising agent to be used in adjuvant strategy for the treatment of APL. PMID: 32169580 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - March 10, 2020 Category: Biochemistry Authors: Esmaeili S, Safaroghli-Azar A, Pourbagheri-Sigaroodi A, Salari S, Gharehbaghian A, Hamidpour M, Bashash D Tags: Int J Biochem Cell Biol Source Type: research

Viroporins and inflammasomes: a key to understand virus-induced inflammation.
Abstract Viroporins are virus encoded proteins that alter membrane permeability and can trigger subsequent cellular signals. Oligomerization of viroporin subunits results in formation of a hydrophilic pore which facilitates ion transport across host cell membranes. These viral channel proteins may be involved in different stages of the virus infection cycle. Inflammasomes are large multimolecular complexes best recognized for their ability to control activation of caspase-1, which in turn regulates the maturation of interleukin-1 β (IL-1β) and interleukin 18 (IL-18). IL-1β was originally identified ...
Source: The International Journal of Biochemistry and Cell Biology - March 7, 2020 Category: Biochemistry Authors: Farag NS, Breitinger U, Breitinger HG, El Azizi MA Tags: Int J Biochem Cell Biol Source Type: research

Neuroscience in the heart: recent advances in neurocardiac communication and its role in cardiac arrhythmias.
Abstract Autonomic nervous system dysregulation is involved in the pathophysiology of multiple cardiac arrhythmias, and therefore modulating sympathetic or parasympathetic input to the heart provides novel therapeutic options for arrhythmia management. Examples include decreasing intrinsic cardiac neuron communication, patterned vagal nerve stimulation, denervation, and blockade of post-ganglionic neurons. However, lessons from ventricular arrhythmias, where increased sympathetic activity and vagal rebound activity both amplify arrhythmia risk, stress the importance of understanding the regulatory mechanisms that ...
Source: The International Journal of Biochemistry and Cell Biology - March 6, 2020 Category: Biochemistry Authors: Winbo A, Ashton JL, Montgomery JM Tags: Int J Biochem Cell Biol Source Type: research

Acid/bile exposure triggers TRAIL-mediated apoptosis in esophageal cancer cells by suppressing the decoy receptors and c-FLIPR.
In this study, we simulated GERD episodes in vitro by exposing the cancer cells to acid or acid/bile combination and found that the cancer cells lived through acid attacks by expression of the decoy receptors and c-FLIPR but died of TRAIL-mediated apoptosis when bile salts were present. Further investigation revealed that acid/bile exposure downregulated the decoy receptors and thereby facilitated TRAIL signaling; meantime, it inhibited protein kinase C activity and thus expedited c-FLIPR degradation, allowing apoptosis to take place. PMID: 32135301 [PubMed - as supplied by publisher] (Source: The International Journa...
Source: The International Journal of Biochemistry and Cell Biology - March 2, 2020 Category: Biochemistry Authors: Meng X, Chang Z, Che N, Wu J, Dang T, Chai J Tags: Int J Biochem Cell Biol Source Type: research

Statins reduce vascular inflammation in atherogenesis: a review of underlying molecular mechanisms.
Abstract Chronic inflammation enhances the detrimental role of dyslipidaemia during atherogenesis. Statins are among the most effective anti-atherosclerotic medications, being able to impact on both cardiovascular morbidity and mortality. Although these molecules have been first described as lipid-lowering medications, several lines of evidence suggest additional benefits through their "pleiotropic" anti-atherosclerotic activities. Specifically, statins can modulate vascular atherosclerotic inflammation by directly improving functions of endothelial cells, vascular smooth muscle cells, platelets, and imm...
Source: The International Journal of Biochemistry and Cell Biology - February 29, 2020 Category: Biochemistry Authors: Liberale L, Carbone F, Montecucco F, Sahebkar A Tags: Int J Biochem Cell Biol Source Type: research

Pan-HDAC inhibitor panobinostat, as a single agent or in combination with PI3K inhibitor, induces apoptosis in APL cells: an emerging approach to overcome MSC-induced resistance.
Abstract The emergence of the drugs targeting epigenetic alterations has brought an optimistic outlook for cancer patients and probably put an end into the devastating effects of the disease. Given to the prominent involvement of histone deacetylase (HDAC) enzymes in the formation of neoplastic nature of acute promyelocytic leukemia (APL), this study was aimed to evaluate the suppressive effect of pan-HDAC inhibitor panobinostat on both NB4 and primary APL patients cells, either in the context of mono- or co-culture with mesenchymal stem cells (MSC). Panobinostat effectively reduced the survival of APL cells; howe...
Source: The International Journal of Biochemistry and Cell Biology - February 28, 2020 Category: Biochemistry Authors: Mosleh M, Safaroghli-Azar A, Bashash D Tags: Int J Biochem Cell Biol Source Type: research

Inhibition of profibrotic signalling enhances the 5-azacytidine-induced reprogramming of fibroblasts into cardiomyocytes.
In this study, we treated CFs with 5-aza for 24 h and added TGF-β inhibitor A83-01 for 2 weeks in vitro to investigate the effect of inhibiting fibrosis on myocardial differentiation. Inhibition of TGF-β1 activity with A83-01 significantly decreased the expressions of collagen III and α-SMA and increased the expression of myocardial specific marker cTnT and gap junction protein Cx43 in CFs, enhanced cardiac reprogramming as opposed to 2 weeks with 5-aza alone. Transcriptome and quantitative real-time reverse transcription-polymerase chain reaction analysis at the 14th day postinduction of A83-01 reve...
Source: The International Journal of Biochemistry and Cell Biology - February 27, 2020 Category: Biochemistry Authors: Jia Y, Chang Y, Sun P, Li H, Guo Z Tags: Int J Biochem Cell Biol Source Type: research

Downregulation of METTL14 increases apoptosis and autophagy induced by cisplatin in pancreatic cancer cells.
Abstract Pancreatic cancer is a leading cause of cancer-related death worldwide. Cisplatin is an essential drug treating patients with BRCA1/2 or PALB2 mutations. Whether other genetic determinants of cisplatin sensitivity exist and their underlying mechanisms remain unclear. Immunohistochemistry was used to determine METTL14 expression in pancreatic cancer tissues and non-tumoural tissues. Cell proliferation was detected with CCK-8 assays. Apoptosis was analysed via Western blotting and flow cytometry, and autophagy was analysed via Western blotting and immunofluorescence. In this work, we found higher METTL14 ex...
Source: The International Journal of Biochemistry and Cell Biology - February 22, 2020 Category: Biochemistry Authors: Kong F, Liu X, Zhou Y, Hou X, He J, Li Q, Miao X, Yang L Tags: Int J Biochem Cell Biol Source Type: research

ROS Enhances the Cytotoxicity of Cisplatin by Inducing Apoptosis and Autophagy in Tongue Squamous Cell Carcinoma Cells.
Abstract Cisplatin is one of the most widely used anticancer agents for patients with tongue squamous cell carcinoma (TSCC), but its efficacy is limited by chemoresistance. Accumulated evidence has demonstrated that reactive oxygen species (ROS) plays a critical role in multiple tumor chemotherapy resistance. In the present study, we aimed to investigate the role of ROS in cisplatin resistance of TSCC and explore its underlying molecular mechanism in vitro. Our results showed that pre-treatment with ROS scavenger N-acetylcysteine reduced cisplatin-induced cytotoxicity, autophagy, and apoptosis in TSCC cells. Down-...
Source: The International Journal of Biochemistry and Cell Biology - February 22, 2020 Category: Biochemistry Authors: Xue DF, Pan ST, Huang G, Qiu JX Tags: Int J Biochem Cell Biol Source Type: research

Bcl-xL inhibits PINK1/Parkin-dependent mitophagy by preventing mitochondrial Parkin accumulation.
This report aims to explore how Bcl-xL, a Bcl-2 family protein, regulates PINK1/Parkin-dependent mitophagy. Compared with the Hela cells expressing Parkin alone, co-expression of Bcl-xL significantly inhibited CCCP (Carbonyl cyanide 3- chlorophenylhydrazone)-induced mitochondrial Parkin accumulation and mitophagy. Western blotting analysis illustrated that over-expressed Bcl-xL inhibited CCCP-induced decrease of mitochondrial proteins in Parkin over-expressed cells. Fluorescence loss in photobleaching (FLIP) analyses demonstrated that Bcl-xL inhibited the CCCP-induced translocation of Parkin into mitochondria not by retrot...
Source: The International Journal of Biochemistry and Cell Biology - February 20, 2020 Category: Biochemistry Authors: Yu S, Du M, Yin A, Mai Z, Wang Y, Zhao M, Wang X, Chen T Tags: Int J Biochem Cell Biol Source Type: research

Circular RNA hsa_circ_0076906 competes with OGN for miR-1305 biding site to alleviate the progression of osteoporosis.
In this study, we described a previously found circular RNA, circ_0076906, to bind miR-1305 and regulate its target gene, Osteoglycin (OGN), thus regulate osteogenic differentiation of hMSCs and alleviate the progression of osteoporosis. Osteogenic differentiation induced in hMSCs; qRT-PCR and western blot to examine the expressions of mRNAs and proteins; Alkaline phosphatase activity and Alizarin red staining to examine bone formation; luciferase report experiments to detect the interaction between molecules; and the nuclear/cytoplasm separation of cells. 1. Circ_0076906 was induced in osteogenic differentiation; 2. Circ_...
Source: The International Journal of Biochemistry and Cell Biology - February 19, 2020 Category: Biochemistry Authors: Wen J, Guan Z, Yu B, Guo J, Shi Y, Hu L Tags: Int J Biochem Cell Biol Source Type: research

TMPO-AS1/miR-98-5p/EBF1 feedback loop contributes to the progression of bladder cancer.
In this study, high expression of TMPO-AS1 was revealed in BCa tissues and cell lines, and TMPO-AS1 predicted poor prognosis. Moreover, TMPO-AS1 facilitated cell growth. Additionally, TMPO-AS1 also boosted the migration and invasion of BCa cells. Mechanistically, overexpressed EBF transcription factor 1 (EBF1) in BCa cell was verified to promote the transcription of TMPO-AS1. Later, we found that TMPO-AS1 was a cytoplasmic RNA and could sponge miR-98-5p. Besides, it was validated that EBF1 is a target gene of miR-98-5p and negatively correlated with miR-98-5p in terms of expression level. According to the results of rescue...
Source: The International Journal of Biochemistry and Cell Biology - February 19, 2020 Category: Biochemistry Authors: Luo H, Yang L, Liu C, Wang X, Dong Q, Liu L, Wei Q Tags: Int J Biochem Cell Biol Source Type: research

The primary cilia in diabetic kidney disease: a tubulocentric view?
Abstract Diabetic kidney disease is growing exponentially. This review aims to discuss alternate therapeutic approaches beyond the glomerulocentric view and to consider a novel tubulocentric approach with focus on the primary cilia. Renin-angiotensin-aldosterone system blockade to decrease glomerular capillary pressure and prevent albuminuria has been the mainstay of treatment for diabetic and non-diabetic proteinuric kidney disease. Landmark clinical trials have also shown cardiorenal benefit with sodium-glucose linked co-transporter 2 inhibitors and glucagon-like peptide 1 receptor analogues in patients with typ...
Source: The International Journal of Biochemistry and Cell Biology - February 15, 2020 Category: Biochemistry Authors: Panchapakesan U, Pollock C Tags: Int J Biochem Cell Biol Source Type: research

Protein-based direct reprogramming of fibroblasts to neuronal cells using 30Kc19 protein and transcription factor Ascl1.
Abstract Direct reprogramming of non-neural lineages to functional neurons holds great potential for neural development, neurological disease modeling, and regenerative medicine. Recent work has shown that single transcription factor Ascl1 can directly reprogram fibroblasts into neuronal cells under co-culture system with glial cells. It is confirmed that Ascl1 is the key driver in the reprogramming of induced neuronal cells (iNCs). However, most reprogramming methods use genetic materials and/or potentially mutagenic molecules to generate iNCs. Herein, we used 30Kc19 protein as a novel fusion partner of transcrip...
Source: The International Journal of Biochemistry and Cell Biology - February 11, 2020 Category: Biochemistry Authors: Ryu J, Hwang NS, Park HH, Park TH Tags: Int J Biochem Cell Biol Source Type: research

Loss of G-protein-signaling modulator 2 accelerates proliferation of lung adenocarcinoma via EGFR signaling pathway.
In this study, it was found that GPSM2 correlates with clinicopathological features and patient's prognosis in LUAD. Knocking down GPSM2 promoted LUAD cell proliferation in vitro and in vivo. Mechanistically, it was demonstrated that GPSM2 knockdown accelerates cell proliferation via the EGFR pathway. These results confirmed that GPSM2 played an important role in LUAD. Moreover, GPSM2, as an independent prognostic factor, may serve as a potential drug target and prognostic biomarker in LUAD. PMID: 32058048 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - February 11, 2020 Category: Biochemistry Authors: Deng M, Liu B, Zhang Z, Chen Y, Wang Y, Wang X, Lv Q, Yang X, Hou K, Che X, Qu X, Liu Y, Zhang Y, Hu X Tags: Int J Biochem Cell Biol Source Type: research

Cadmium induces mitochondrial ROS inactivation of XIAP pathway leading to apoptosis in neuronal cells.
Abstract Cadmium (Cd), a heavy metal pollutant, contributes to neurodegenerative disorders. Recently, we have demonstrated that Cd-induced reactive oxygen species (ROS) causes apoptosis in neuronal cells. Whether X-linked inhibitor of apoptosis protein (XIAP) is involved in Cd-induced ROS-dependent neuronal apoptosis remains unclear. Here, we show that Cd-induced ROS reduced the expression of XIAP, which resulted in up-regulation of murine double minute 2 homolog (MDM2) and down-regulation of p53, leading to apoptosis in PC12 cells and primary neurons. Inhibition of MDM2 with Nutlin-3a reversed Cd-induced reductio...
Source: The International Journal of Biochemistry and Cell Biology - February 5, 2020 Category: Biochemistry Authors: Zhao R, Yu Q, Hou L, Dong X, Zhang H, Chen X, Zhou Z, Ma J, Huang S, Chen L Tags: Int J Biochem Cell Biol Source Type: research

Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells.
Abstract Drug resistance and recurrence are significant contributors to the poor prognosis of serous ovarian cancer (SOC). Radiotherapy is primarily used for the treatment of cancer recurrence; however, it is rarely applied in cases of SOC. Ku86, also known as XRCC5(X-ray repair cross complementing 5), has rarely been reported in the radiotherapy of SOC. Therefore, this study aimed to investigate the role of Ku86 in the development of SOC and in radiotherapy sensitivity induced by X-ray. In vitro experiments and database analysis showed significantly elevated expression of Ku86 in SOC. Further, after down-regulati...
Source: The International Journal of Biochemistry and Cell Biology - February 3, 2020 Category: Biochemistry Authors: Ma Q, Kai J, Liu Y, Tong Y, Xie S, Zheng H, Wang Y, Guo L, Lu R Tags: Int J Biochem Cell Biol Source Type: research

Cytochrome c phosphorylation: Control of mitochondrial electron transport chain flux and apoptosis.
n M Abstract Cytochrome c (Cytc)1is a cellular life and death decision molecule that regulates cellular energy supply and apoptosis through tissue specific post-translational modifications. Cytc is an electron carrier in the mitochondrial electron transport chain (ETC) and thus central for aerobic energy production. Under conditions of cellular stress, Cytc release from the mitochondria is a committing step for apoptosis, leading to apoptosome formation, caspase activation, and cell death. Recently, Cytc was shown to be a target of cellular signaling pathways that regulate the functions of Cytc by tissue-specific ...
Source: The International Journal of Biochemistry and Cell Biology - February 2, 2020 Category: Biochemistry Authors: Kalpage HA, Wan J, Morse PT, Zurek MP, Turner AA, Khobeir A, Yazdi N, Hakim L, Liu J, Vaishnav A, Sanderson TH, Recanati MA, Grossman LI, Lee I, Edwards BFP, Hüttemann M Tags: Int J Biochem Cell Biol Source Type: research

Angiotensin II inhibits osteogenic differentiation of isolated synoviocytes by increasing DKK-1 expression.
This study aims to investigate the effect of angiotensin II (Ang II) on osteogenic differentiation of synoviocytes and the underlying mechanism. Ang II was showed to inhibite osteogenic differentiation of synoviocytes, which was mitigated by a Dickkopf-1 (DKK-1) inhibitor. DKK-1 was upregulated by Ang II, which was weakened by the Ang II type 1 receptor (AT1R) blocker, reactive oxygen species (ROS) scavenger, and p38 inhibitor. Ang II increased the levels of AT1R, ROS, and NADPH oxidase (NOX), and the upregulations were mitigated by the AT1R blocker or NOX inhibitor. Furthermore, Ang II activated the p38 pathway, which was...
Source: The International Journal of Biochemistry and Cell Biology - January 31, 2020 Category: Biochemistry Authors: Zhang Y, Ding H, Song Q, Wang Z, Yuan W, Ren Y, Zhao Z, Wang C Tags: Int J Biochem Cell Biol Source Type: research

Corrigendum to "Mitochondrial oxidative phosphorylation is impaired in TALLYHO mice, a new obesity and type 2 diabetes animal model" [Int. J. Biochem. Cell Biol. 116 (2019) 1-11].
Corrigendum to "Mitochondrial oxidative phosphorylation is impaired in TALLYHO mice, a new obesity and type 2 diabetes animal model" [Int. J. Biochem. Cell Biol. 116 (2019) 1-11]. Int J Biochem Cell Biol. 2020 Jan 30;:105699 Authors: Hunter CA, Kartal F, Koc ZC, Murphy T, Kim JH, Denvir J, Koc EC PMID: 32008916 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - January 30, 2020 Category: Biochemistry Authors: Hunter CA, Kartal F, Koc ZC, Murphy T, Kim JH, Denvir J, Koc EC Tags: Int J Biochem Cell Biol Source Type: research

(-)-Asarinin inhibits mast cells activation as a Src family kinase inhibitor.
In conclusion, Asa may serve as a potential novel Src family kinase inhibitor to inhibit IgE-dependent andIgE-independent allergic reaction and treat anaphylactic disease. PMID: 32007602 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - January 30, 2020 Category: Biochemistry Authors: Hou Y, Hu T, Wei D, Gao J, Che D, Wang X, Wang C, He H Tags: Int J Biochem Cell Biol Source Type: research

Mechanism of miR-222 and miR-126 regulation and its role in asbestos-induced malignancy.
Abstract MiR-222 and miR-126 are associated with asbestos exposure and the ensuing malignancy, but the mechanism(s) of their regulation remain unclear. We evaluated the mechanism by which asbestos regulates miR-222 and miR-126 expression in the context of cancer etiology. An 'in vitro' model of carcinogen-induced cell transformation was used based on exposing bronchial epithelium BEAS-2B cells to three different carcinogens including asbestos. Involvement of the EGFR pathway and the role of epigenetics have been investigated in carcinogen-transformed cells and in malignant mesothelioma, a neoplastic disease associ...
Source: The International Journal of Biochemistry and Cell Biology - January 29, 2020 Category: Biochemistry Authors: Gaetani S, Monaco F, Alessandrini F, Tagliabracci A, Sabbatini A, Bracci M, Valentino M, Neuzil J, Amati M, Santarelli L, Tomasetti M Tags: Int J Biochem Cell Biol Source Type: research

Transcriptome sequencing reveals fibrotic associated-genes involved in bovine mammary fibroblasts with Staphylococcus aureus.
In this study, we have aimed to determine the transcriptional responses in bovine mammary fibroblasts (BMFBs) induced by S. aureus using bioinformatics analysis to determine whether mRNA expression profile changes between BMFBs activation and quiescence. Established primary BMFBs obtained from healthy Holstein bovine were induced 106 CFU/mL heat-inactivated S. aureus and total RNA was isolated 6 h after treatment. The 574 DEGs were involved in gene ontology (GO) that were immune response, apoptotic process, extracellular region, receptor binding, endopeptidase activity and protein kinase activity et al. The Kyoto En...
Source: The International Journal of Biochemistry and Cell Biology - January 27, 2020 Category: Biochemistry Authors: Miao Z, Ding Y, Zhao N, Chen X, Cheng H, Wang J, Liu Y, Wang F Tags: Int J Biochem Cell Biol Source Type: research

Ubiquitin Ligase LRSAM1 Suppresses Neurodegenerative Diseases Linked Aberrant Proteins Induced Cell Death.
This study will also allow us to better comprehend the problem of proteinopathies linked with aberrant protein accumulation and open new possibilities to better elucidate the molecular mechanisms involved in the pathologies of neurodegeneration and aging. PMID: 31982566 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - January 23, 2020 Category: Biochemistry Authors: Mishra R, Amanullah A, Upadhyay A, Dhiman R, Dubey AR, Singh S, Prasad A, Mishra A Tags: Int J Biochem Cell Biol Source Type: research

Corrigendum to "Targeted gold nanoparticles enable molecular CT imaging of head and neck cancer: An in vivo study" [Int. J. Biochem. Cell Biol. 114 (2019) 105554].
Corrigendum to "Targeted gold nanoparticles enable molecular CT imaging of head and neck cancer: An in vivo study" [Int. J. Biochem. Cell Biol. 114 (2019) 105554]. Int J Biochem Cell Biol. 2020 Jan 22;:105695 Authors: Khademi S, Sarkar S, Shakeri-Zadeh A, Attaran N, Kharrazi S, Ay MR, Azimian H, Ghadiri H PMID: 31982287 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - January 22, 2020 Category: Biochemistry Authors: Khademi S, Sarkar S, Shakeri-Zadeh A, Attaran N, Kharrazi S, Ay MR, Azimian H, Ghadiri H Tags: Int J Biochem Cell Biol Source Type: research

Antioxidant enzymes change in different non-metastatic stages in tumoral and peritumoral tissues of colorectal cancer.
Sastre-Serra J, Pons DG Abstract Antioxidant defences and oxidative stress are related to development, progression and malignancy of colorectal cancer. However, their role in early stages of cancer remains unknown. More and more recent studies have revealed that non-tumour adjacent tissue is not a normal tissue. Thus, our aim was to analyse protein levels of MnSOD (Manganese Superoxide Dismutase), acMnSOD (Acetylated Manganese superoxide Dismutase), SIRT3 (Sirtuin 3), CuZnSOD (Cupper Zinc Superoxide Dismutase), CAT (Catalase), GPx (Glutathione Peroxidase), and GRd (Glutathione Reductase) both in tumour and non-tum...
Source: The International Journal of Biochemistry and Cell Biology - January 22, 2020 Category: Biochemistry Authors: Gaya-Bover A, Hernández-López R, Alorda-Clara M, de la Rosa JMI, Falcó E, Fernández T, Company MM, Torrens-Mas M, Roca P, Oliver J, Sastre-Serra J, Pons DG Tags: Int J Biochem Cell Biol Source Type: research

Inner mitochondrial membrane compartmentalization: Dynamics across scales.
Abstract Mitochondria are known as dynamic organelles that fuse and divide under the control of certain proteins. These dynamics are important to shape mitochondria, maintain a healthy mitochondrial population, and enable physiological adaptations, to name just a few key processes. We are less aware that mitochondrial membrane lipids and proteins also exhibit dynamics in terms of lateral mobility and translocation. This single molecule dynamics is equally important for the above processes as it enables interaction with other proteins and complexes. Here we discuss some mitochondrial proteins and the role of their ...
Source: The International Journal of Biochemistry and Cell Biology - January 10, 2020 Category: Biochemistry Authors: Busch KB Tags: Int J Biochem Cell Biol Source Type: research

Comparative peptidome profiling reveals critical roles for peptides in the pathology of pancreatic cancer.
CONCLUSION: P1DG and its precursor GAPDH may be closely related to the proliferation, migration and invasion of pancreatic cancer. Peptidomics can aid in understanding the pathogenesis of pancreatic cancer more comprehensively. PMID: 31927104 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - January 9, 2020 Category: Biochemistry Authors: Li X, Li J, Zhang B, Gu Y, Li Q, Gu G, Xiong J, Li Y, Yang X, Qian Z Tags: Int J Biochem Cell Biol Source Type: research

PRMT7 promotes the growth of renal cell carcinoma through modulating the β-catenin/C-MYC axis.
In conclusion, our study demonstrates that overexpressed PRMT7 in ccRCC cells acts as an oncogene to promote the growth of renal cell carcinoma through regulating the β-catenin/C-MYC axis, thereby providing new strategies and targets for the treatment of ccRCC patients. PMID: 31926310 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - January 8, 2020 Category: Biochemistry Authors: Liu F, Wan L, Zou H, Pan Z, Zhou W, Lu X Tags: Int J Biochem Cell Biol Source Type: research

Knockdown of CTCF reduces the binding of EZH2 and affects the methylation of the SOCS3 promoter in hepatocellular carcinoma.
In this study, Using TCGA HCC dataset, and detecting HCC tissue specimens and cell lines, we found that SOCS3 expression in HCC was inversely related to that of EZH2, and depended on its promoter methylation status. CTCF, Vigilin, EZH2 and H3K27me3 were enriched at CTCF and EZH2 binding sites on the methylated SOCS3 gene promoter. The depletion of CTCF did not affect expression of EZH2 and DNMT1, but decrease recruitment of CTCF, Vigilin, EZH2 and H3K27me3. Further, knockdown of CTCF led to a loss of methylation of the methylated SOCS3 promoter, which sequentially increased the expression of SOCS3 and decreased the express...
Source: The International Journal of Biochemistry and Cell Biology - January 5, 2020 Category: Biochemistry Authors: Wei L, Liu Q, Huang Y, Liu Z, Zhao R, Li B, Zhang J, Sun C, Gao B, Ding X, Yu X, He J, Sun A, Qin Y Tags: Int J Biochem Cell Biol Source Type: research

Regulation of efferocytosis by caspase-dependent apoptotic cell death in atherosclerosis.
Abstract During the growing process of the atherosclerotic lesions, lipid-filled macrophage foam cells form, accumulate, and ultimately undergo apoptotic death. If the apoptotic foam cells are not timely removed, they may undergo secondary necrosis, and form a necrotic lipid core which renders the plaque unstable and susceptible to rupture. Therefore, the non-lipid-filled fellow macrophages, as the main phagocytic cells in atherosclerotic lesions, need to effectively remove the apoptotic foam cells. In general, in apoptotic macrophages, caspases are the central regulators of several key processes required for thei...
Source: The International Journal of Biochemistry and Cell Biology - January 3, 2020 Category: Biochemistry Authors: Tajbakhsh A, Kovanen PT, Rezaee M, Banach M, Moallem SA, Sahebkar A Tags: Int J Biochem Cell Biol Source Type: research