RETRACTED: Down-regulation of miR-373 increases the radiosensitivity of lung cancer cells by targeting TIMP2.
PMID: 31186209 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - June 8, 2019 Category: Biochemistry Authors: Guo Y, Jiang Y, Sang M, Xu C Tags: Int J Biochem Cell Biol Source Type: research

N-glycan-calnexin interactions in human factor VII secretion and deficiency.
In this study, we tested the hypothesis that N-glycans in the FVII protease domain mediate calnexin-assisted protein folding and that naturally occurring F7 mutations abolishing N-glycosylation impair FVII secretion. We expressed human FVII wild-type (WT) and mutant proteins lacking one or both N-glycosylation sites in HEK293 and HepG2 cells in the presence or absence of a glucosidase inhibitor. FVII expression, secretion and binding to endoplasmic reticulum chaperones were examined by immune staining, co-immunoprecipitation, Western blotting, and ELISA. We found that N-glycosylation at N360 in the protease domain, but not...
Source: The International Journal of Biochemistry and Cell Biology - June 8, 2019 Category: Biochemistry Authors: Wang H, Wang L, Li S, Dong N, Wu Q Tags: Int J Biochem Cell Biol Source Type: research

Editorial - Splicing and Alternative Splicing.
s HMB PMID: 31175995 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - June 5, 2019 Category: Biochemistry Authors: Capelo-Martinez JL, Chabot B, Espiño CL, Carreira Dos Santos HMB Tags: Int J Biochem Cell Biol Source Type: research

Resveratrol up-regulates ATP2A3 gene expression in breast cancer cell lines through epigenetic mechanisms.
arain-Herzberg Á Abstract Resveratrol (RSV) is a phytoestrogen which has been related to chemoprevention of several types of cancer. In this work, we show up to a 6-fold increased expression of ATP2A3 gene induced by RSV that triggers apoptosis and changes of intracellular Ca2+ management in MCF-7 and MDA-MB-231 breast cancer cell lines. We explored epigenetic mechanisms for that RSV-induced ATP2A3 up-regulation. The results indicate that RSV-induced ATP2A3 up-regulation correlates with about 50% of reduced HDAC activity and reduced nuclear HDAC2 expression and occupancy on ATP2A3 promoter, increasing the g...
Source: The International Journal of Biochemistry and Cell Biology - June 4, 2019 Category: Biochemistry Authors: Izquierdo-Torres E, Hernández-Oliveras A, Meneses-Morales I, Rodríguez G, Fuentes-García G, Zarain-Herzberg Á Tags: Int J Biochem Cell Biol Source Type: research

LncRNA XIST promotes pancreatic cancer migration, invasion and EMT by sponging miR-429 to modulate ZEB1 expression.
Abstract Pancreatic cancer (PC) has become a worldwide malignancy accompanied by high metastasis and extremely poor prognosis. The critical roles of long non-coding RNAs (lncRNAs) in PC are generally summarized as molecular sponges of microRNAs (miRNAs). We intended to investigate the biological function and mechanism of lncRNA X-inactive specific transcript (XIST) in PC progression, especially in PC cell migration and invasion. qPCR was applied to detect the expression levels of XIST and miR-429 in PC tissues and cell lines. The roles of XIST and miR-429 on PC cell migration, invasion and epithelial-mesenchymal t...
Source: The International Journal of Biochemistry and Cell Biology - June 1, 2019 Category: Biochemistry Authors: Shen J, Hong L, Yu D, Cao T, Zhou Z, He S Tags: Int J Biochem Cell Biol Source Type: research

Corrigendum to "MicroRNA-33a and let-7e inhibit human colorectal cancer progression by targeting ST8SIA1" [Int. J. Biochem. Cell Biol. 90 (2017) 48-58].
Corrigendum to "MicroRNA-33a and let-7e inhibit human colorectal cancer progression by targeting ST8SIA1" [Int. J. Biochem. Cell Biol. 90 (2017) 48-58]. Int J Biochem Cell Biol. 2019 May 28;: Authors: Shan Y, Liu Y, Zhao L, Liu B, Li Y, Jia L PMID: 31151731 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - May 28, 2019 Category: Biochemistry Authors: Shan Y, Liu Y, Zhao L, Liu B, Li Y, Jia L Tags: Int J Biochem Cell Biol Source Type: research

Inhibition of dendritic spine extension through acrolein conjugation with α-, β-tubulin proteins.
Inhibition of dendritic spine extension through acrolein conjugation with α-, β-tubulin proteins. Int J Biochem Cell Biol. 2019 May 28;: Authors: Uemura T, Suzuki T, Ko K, Watanabe K, Dohmae N, Sakamoto A, Terui Y, Toida T, Kashiwagi K, Igarashi K Abstract We have recently found that conjugation of acrolein with a 50 kDa protein(s) is strongly associated with tissue damage during brain infarction. In the current study, the identity and function of the 50 kDa protein(s) conjugated with acrolein during brain infarction were investigated. The 50 kDa protein(s) conjugated w...
Source: The International Journal of Biochemistry and Cell Biology - May 28, 2019 Category: Biochemistry Authors: Uemura T, Suzuki T, Ko K, Watanabe K, Dohmae N, Sakamoto A, Terui Y, Toida T, Kashiwagi K, Igarashi K Tags: Int J Biochem Cell Biol Source Type: research

Androgen upregulates NR4A1 via the TFAP2A and ETS signaling networks.
In conclusion, this study presents the first description that TFAP2A and ETS family signaling networks are involved in the androgen-mediated transcriptional regulation of NR4A1, which contributes to the understanding of the molecular mechanisms involved in the TFAP2A-NR4A1 and ETS-NR4A1 signaling networks in PCOS. PMID: 31146003 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - May 27, 2019 Category: Biochemistry Authors: Song J, Diao F, Ma X, Xu S, Cui Y, Jiang S, Liu J Tags: Int J Biochem Cell Biol Source Type: research

Histone deacetylase inhibitors promote ATP2A3 gene expression in hepatocellular carcinoma cells: p300 as a transcriptional regulator.
antiago-García J Abstract Sarco(endo)plasmic reticulum Ca2+-ATPases (SERCA) expression is reduced or absent in several types of cancer and cancer cell lines; however, their expression and regulation in hepatocellular carcinoma (HCC) are unknown. Histone deacetylase inhibitors (HDACi) increase SERCA3 mRNA expression in gastric and breast cancer cell lines by increasing H3K9ac and binding of Sp1 and Sp3 transcription factors to the promoter; however, the molecular mechanism is not fully understood. Our results show that ATP2A3 (SERCA3) gene expression is decreased in human HCC samples and rat HCC AS-30D cells...
Source: The International Journal of Biochemistry and Cell Biology - May 27, 2019 Category: Biochemistry Authors: Hernández-Oliveras A, Izquierdo-Torres E, Meneses-Morales I, Rodríguez G, Zarain-Herzberg Á, Santiago-García J Tags: Int J Biochem Cell Biol Source Type: research

Dual Effects of Gossypol on Human Hepatocellular Carcinoma via Endoplasmic Reticulum Stress and Autophagy.
In this study, we found gossypol inhibited HCC cells in vitro and in vivo. Typical apoptosis was induced in HCC cells. Dilated ER and autophagosomes were observed by electron microscopy, and the activation of the unfolded protein response and autophagy markers suggested that gossypol induced both ER stress and autophagy. C/EBP homologous protein was the key factor that led to apoptotic cell death, whereas inositol-requiring enzyme 1α and eukaryotic initiation factor 2α played a protective role. Autophagy protected the cells from ER stress-related apoptosis. Both in vitro and in vivo studies indicated that inhib...
Source: The International Journal of Biochemistry and Cell Biology - May 22, 2019 Category: Biochemistry Authors: Zhang G, Wang Z, Chen W, Cao Y, Wu J, Qiang G, Ji A, Wu J, Jiang C Tags: Int J Biochem Cell Biol Source Type: research

Studying the ShcD and ERK interaction under acute oxidative stress conditions in melanoma cells.
Abstract The newly identified melanoma-associated adaptor ShcD was found to translocate to the nucleus upon hydrogen peroxide treatment. Therefore, the aim of this study was to identify the ShcD network in melanoma cells under oxidative stress. LC-MS/MS and GFP-trap were performed to study the ShcD phosphorylation status during acute severe oxidative stress. ShcD was found to be phosphorylated at threonine-159 (Thr159) in response to 5 mM H2O2 treatment. The GPS 2.1 phosphorylation prediction program predicted that the Thr159Pro motif, housed in the N-terminus of the ShcD-CH2 domain, is a potential phosphor...
Source: The International Journal of Biochemistry and Cell Biology - May 20, 2019 Category: Biochemistry Authors: Ahmed SBM, Amer S, Emad M, Rahmani M, Prigent SA Tags: Int J Biochem Cell Biol Source Type: research

Evaluation of differentially expressed microRNAs in vitrified oocytes by next generation sequencing.
Abstract MicroRNAs (miRNAs) play crucial roles in gametogenesis and embryo development. The present study was undertaken to identify differentially expressed miRNAs and explore their functions in oocyte vitrification. Small RNA sequencing data revealed that 22 miRNAs were differentially expressed in vitrified oocytes compared with that of the fresh counterparts. The potential target genes for the differentially expressed miRNAs were enriched in "anatomical development" in biological process, "cell" in cellular components, and "protein binding" in molecular functions by gene ontology a...
Source: The International Journal of Biochemistry and Cell Biology - May 17, 2019 Category: Biochemistry Authors: Li J, Yang X, Liu F, Song Y, Liu Y Tags: Int J Biochem Cell Biol Source Type: research

LncRNA H19 promotes epithelial mesenchymal transition and metastasis of esophageal cancer via STAT3/EZH2 axis.
CONCLUSIONS: lncRNA H19 facilitates EMT and metastasis of EC through let-7c/STAT3/EZH2/β-catenin axis. PMID: 31102664 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - May 15, 2019 Category: Biochemistry Authors: Chen MJ, Deng J, Chen C, Hu W, Yuan YC, Xia ZK Tags: Int J Biochem Cell Biol Source Type: research

Cdc42 regulate the apoptotic cell death required for planarian epidermal regeneration and homeostasis.
Abstract Rho GTPases have been shown previously to play important roles in several cellular processes by regulating the organization of the actin and microtubule cytoskeletons. However, the mechanisms of Rho GTPases that integrate the cellular responses during regeneration have not been thoroughly elucidated. The planarian flatworm, which contains a large number of adult somatic stem cells (neoblasts), is a unique model to study stem cell lineage development in vivo. Here, we focus on cdc42, which is an extensively characterized member among Rho GTPases. We found that cdc42 is required for the maintenance of epide...
Source: The International Journal of Biochemistry and Cell Biology - May 15, 2019 Category: Biochemistry Authors: Yujia S, Tingting G, Jiaxin L, Saisai Z, Zhitai H, Qingnan T, Shoutao Z Tags: Int J Biochem Cell Biol Source Type: research

Microvesicles derived from human Wharton's Jelly mesenchymal stem cells ameliorate acute lung injury partly mediated by hepatocyte growth factor.
Abstract Several studies have highlighted the underlying role of mesenchymal stem cells microvesicles (MSC-MVs) in acute lung injury (ALI). Hepatocyte growth factor (HGF) derived from MSC-MVs is partly involved in their therapeutic effects; however, the detailed mechanism remains unclear. MVs were isolated from human Wharton's Jelly MSCs. The rat model of ALI was established by intratracheal instillation of bleomycin (BLM). A co-culture model of alveolar epithelial cells or pulmonary endothelial cells and MSC-MVs was utilized. Total protein content in bronchoalveolar lavage fluid (BALF) was determined by bicinchon...
Source: The International Journal of Biochemistry and Cell Biology - May 14, 2019 Category: Biochemistry Authors: Chen W, Wang S, Xiang H, Liu J, Zhang Y, Zhou S, Du T, Shan L Tags: Int J Biochem Cell Biol Source Type: research

Massive Volumetric Imaging of Cleared Tissue: The Necessary Tools to be Successful.
Abstract Recent technological innovations in high-speed microscopes, tissue clearing methods, optics and computation have made it possible to image enormous biologic volumes. These techniques are applicable to a broad range of biomedical disciplines and are becoming a necessary tool for exploring biologic systems that extend beyond cellular microenvironments to entire organs and organisms. Here we broadly discuss the tool sets that are available for cleared tissue imaging and how various choices may influence the planning and feasibility of an experiment. PMID: 31085331 [PubMed - as supplied by publisher] (So...
Source: The International Journal of Biochemistry and Cell Biology - May 11, 2019 Category: Biochemistry Authors: Watson AM, Watkins SC Tags: Int J Biochem Cell Biol Source Type: research

Heat shock protein family B member 1 facilitates ezrin activation to control cell migration in esophageal squamous cell carcinoma.
Abstract Ezrin plays an important role in the development and progression of human esophageal squamous cell carcinoma (ESCC), providing a link between the cortical actin cytoskeleton and the plasma membrane to govern membrane structure and protrusions. However, the mechanism by which ezrin is activated still remains unknown in ESCC. Here, we identify a novel interaction between ezrin and heat shock protein family B (small) member 1 (HSPB1) in ESCC cells by mass spectroscopy and co-immunoprecipitation. HSPB1 only interacts with inactive ezrin and binds to the α-helical coiled coil region of ezrin. Knockdown o...
Source: The International Journal of Biochemistry and Cell Biology - May 10, 2019 Category: Biochemistry Authors: Xie YH, Li LY, He JZ, Xu XE, Liao LD, Zhang Q, Xie JJ, Xu LY, Li EM Tags: Int J Biochem Cell Biol Source Type: research

GPER1 influences cellular homeostasis and cytostatic drug resistance via influencing long chain ceramide synthesis in breast cancer cells.
ösch S Abstract The G protein-coupled estrogen receptor 1 (GPER1) is involved in the regulation of physiological processes such as cellular growth and proliferation, but also in pathophysiological processes such as tumor development. The role of GPER1 in breast cancer is contradictory. Therefore, we investigated the influence of GPER1 overexpression on cellular processes in MCF-7 breast cancer cells. GPER1 overexpression leads to a cell cycle arrest in the G1 phase, induction of autophagy and reduced proliferation. Reduced proliferation was accompanied by a reduced basal respiration and reduced glycolysis rat...
Source: The International Journal of Biochemistry and Cell Biology - May 10, 2019 Category: Biochemistry Authors: Wegner MS, Gruber L, Schömel N, Trautmann S, Brachtendorf S, Fuhrmann D, Schreiber Y, Olesch C, Brüne B, Geisslinger G, Grösch S Tags: Int J Biochem Cell Biol Source Type: research

Metformin INHIBITS β-Catenin PHOSPHORYLATION ON SER-552 through an AMPK/PI3K/AKT Pathway in colorectal cancer cells.
Metformin INHIBITS β-Catenin PHOSPHORYLATION ON SER-552 through an AMPK/PI3K/AKT Pathway in colorectal cancer cells. Int J Biochem Cell Biol. 2019 May 10;: Authors: Amable G, Martínez-León E, Picco ME, Di Siervi N, Davio C, Rozengurt E, Rey O Abstract Several epidemiologic studies have revealed strong inverse associations between metformin use and risk of colorectal cancer development. Nevertheless, the underlying mechanisms are still uncertain. The Wnt/β-catenin pathway, which plays a central role in intestinal homeostasis and sporadic colorectal cancer development, is regulat...
Source: The International Journal of Biochemistry and Cell Biology - May 10, 2019 Category: Biochemistry Authors: Amable G, Martínez-León E, Picco ME, Di Siervi N, Davio C, Rozengurt E, Rey O Tags: Int J Biochem Cell Biol Source Type: research

CD200 in asthma.
tte E Abstract Constant exposure to foreign particles in the airways requires tight immune regulation in order to maintain sufficient anti-microbial defences, while preventing immunopathological responses that could impair gas exchange. Dysregulation of immunoregulatory pathways has been associated with asthma and allergy. This review will focus on the CD200 regulatory pathway and its role in the asthmatic cascade. CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modula...
Source: The International Journal of Biochemistry and Cell Biology - May 8, 2019 Category: Biochemistry Authors: Lauzon-Joset JF, Marsolais D, Tardif-Pellerin É, Patoine D, Bissonnette E Tags: Int J Biochem Cell Biol Source Type: research

Taurine Protects Against Arsenic Trioxide-Induced Insulin Resistance via ROS-Autophagy Pathway in Skeletal Muscle.
In this study, C57BL/6 mice exposed to As2O3 showed impaired glucose tolerance, decrease in insulin sensitivity and insulin resistance were observed in the skeletal muscle and myotubes of mice that underwent As2O3 treatment. Decreased insulin-stimulated glucose uptake (ISGU) was also shown by the As2O3-treated myotubes. Moreover, the accumulation of ectopic fat in mice skeletal muscle and myotubes was observed after As2O3 treatment. The upregulated expression of autophagy-associated proteins and the increased number of acidic vesicular organelles (AVOs) indicated that autophagy was stimulated in the skeletal muscle and myo...
Source: The International Journal of Biochemistry and Cell Biology - May 3, 2019 Category: Biochemistry Authors: Yang L, Qiu T, Yao X, Jiang L, Wei S, Pei P, Wang Z, Bai J, Liu X, Yang G, Liu S, Sun X Tags: Int J Biochem Cell Biol Source Type: research

Extracellular regulation of airway smooth muscle contraction.
te; Y Abstract The molecular mechanisms governing the contraction of airway smooth muscle have always been at the forefront of asthma research. New extracellular molecules affecting the contraction of airway smooth muscle are steadily being discovered. Although interesting, this is disconcerting for researchers trying to find a mend for the significant part of asthma symptoms caused by contraction. Additional efforts are being deployed to understand the intracellular signaling pathways leading to contraction. The goal being to find common pathways that are essential to convey the contractile signal emanating from ...
Source: The International Journal of Biochemistry and Cell Biology - April 28, 2019 Category: Biochemistry Authors: Khadangi F, Bossé Y Tags: Int J Biochem Cell Biol Source Type: research

Comprehensive circular RNA expression profiles and the tumor-suppressive function of circHIPK3 in ovarian cancer.
CONCLUSIONS: We identified the circRNA expression profile in ovarian cancer tissues and further verified the existence and expression of six randomly selected differentially expressed circRNAs. Besides, we also found that circHIPK3 is an important regulator of ovarian cancer progression. PMID: 31026505 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - April 23, 2019 Category: Biochemistry Authors: Teng F, Xu J, Zhang M, Liu S, Gu Y, Zhang M, Wang X, Ni J, Qian B, Shen R, Jia X Tags: Int J Biochem Cell Biol Source Type: research

Chalcone derivatives as non-canonical ligands of TRPV1.
Abstract Transient receptor potential vanilloid 1 (TRPV1) is a polymodal cation channel activated by heat, voltage, and ligands. Also known as the capsaicin receptor, TRPV1 is expressed in numerous tissues by different cell types, including peripheral sensory fibers where acts as a thermal and chemical detector in nociceptive pathways. TRPV1 channels are able to bind a wide range of ligands, including a number of vanilloid derivatives all modulating channel's activity. When expressed by sensory neurons, activation of TRPV1 channels by heat (>40 °C), capsaicin (sub-micromolar), or acid environment (pH 
Source: The International Journal of Biochemistry and Cell Biology - April 23, 2019 Category: Biochemistry Authors: Benso B, Bustos D, Zarraga MO, Gonzalez W, Caballero J, Brauchi SE Tags: Int J Biochem Cell Biol Source Type: research

Reelin: Diverse roles in central nervous system development, health and disease.
Abstract Over the past 20 years the structure and function of Reelin, an extracellular glycoprotein with a role in cell migration and positioning during development has been elucidated. Originally discovered in mice exhibiting a peculiar gait and hypoplastic cerebellar tissue, Reelin is secreted from Cajal-Retzius neurons during embryonic life and has been shown to act as a stop signal, guiding migrating radial neurons in a gradient-dependent manner. Reelin carries out its function by binding to the receptors, very low-density lipoprotein receptor (VLDLR) and apolipoprotein E receptor 2 (ApoER2) resulting in the p...
Source: The International Journal of Biochemistry and Cell Biology - April 22, 2019 Category: Biochemistry Authors: Armstrong NC, Anderson RC, McDermott KW Tags: Int J Biochem Cell Biol Source Type: research

Psammaplysin F increases the efficacy of bortezomib and sorafenib through regulation of stress granule formation.
Abstract The past few decades have delivered significant improvements in diagnosis and treatment of cancer, however, despite these improvements cancer continues to be a major global health issue requiring the urgent development of new strategies for treatment. Stress granules are cytoplasmic structures that triage gene expression in response to environmental stresses, including chemotherapies, and have been implicated in the development of drug resistance. One novel approach to developing a new anti-cancer strategy involves inhibiting stress granules with compounds derived from natural products. In a previous rapi...
Source: The International Journal of Biochemistry and Cell Biology - April 22, 2019 Category: Biochemistry Authors: Christen KE, Davis RA, Kennedy D Tags: Int J Biochem Cell Biol Source Type: research

Nucleophosmin in leukemia: consequences of anchor loss.
aacute; M, Kuželová K Abstract Nucleophosmin (NPM), one of the most abundant nucleolar proteins, has crucial functions in ribosome biogenesis, cell cycle control, and DNA-damage repair. In human cells, NPM occurs mainly in oligomers. It functions as a chaperone, undergoes numerous interactions and forms part of many protein complexes. Although NPM role in carcinogenesis is not fully elucidated, a variety of tumor suppressor as well as oncogenic activities were described. NPM is overexpressed, fused with other proteins, or mutated in various tumor types. In the acute myeloid leukemia (AML), characteristic m...
Source: The International Journal of Biochemistry and Cell Biology - April 19, 2019 Category: Biochemistry Authors: Brodská B, Šašinková M, Kuželová K Tags: Int J Biochem Cell Biol Source Type: research

Calcium-activated chloride channels: potential targets for antinociceptive therapy.
Abstract The molecular identity of calcium-activated chloride channels (CaCCs) was clarified only some ten years ago when it was linked to the family of "transmembrane proteins of unknown function 16" (TMEM16). Since then, numerous studies have been conducted both to define their role in physiology and identify their biophysical functions. For the latter, the ultrastructural description of mouse TMEM16 A was a breakthrough. CaCCs were functionally described in a number of different tissues including first-order sensory neurons. The activating rise in intracellular calcium concentration can be caus...
Source: The International Journal of Biochemistry and Cell Biology - April 18, 2019 Category: Biochemistry Authors: Salzer I, Boehm S Tags: Int J Biochem Cell Biol Source Type: research

miR-425-5p decreases LncRNA MALAT1 and TUG1 expressions and suppresses tumorigenesis in osteosarcoma via Wnt/ β-catenin signaling pathway.
miR-425-5p decreases LncRNA MALAT1 and TUG1 expressions and suppresses tumorigenesis in osteosarcoma via Wnt/β-catenin signaling pathway. Int J Biochem Cell Biol. 2019 Apr 12;: Authors: Yang G, Zhang C, Wang N, Chen J Abstract Multiple miRNAs have been recognized as critical regulators in osteosarcoma (OS) carcinogenesis. miR-425-5p was demonstrated to be downregulated in the serum of OS patients. However, the detailed roles of miR-425-5p in OS progression and its underlying molecular mechanism are far from being addressed. In our study, the reduced expression of miR-425-5p was downregulated in O...
Source: The International Journal of Biochemistry and Cell Biology - April 12, 2019 Category: Biochemistry Authors: Yang G, Zhang C, Wang N, Chen J Tags: Int J Biochem Cell Biol Source Type: research

A positive feedback loop of miR-30a-5p-WWP1-NF- κB in the regulation of glioma development.
In conclusion, there is a "miR-30a-5p-WWP1-NF-κB" positive feedback loop, which plays an important role in regulating glioma development and might provide a potential therapeutic strategy for treating glioma. PMID: 30978403 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - April 9, 2019 Category: Biochemistry Authors: Zhao P, Wang M, An J, Sun H, Li T, Li D Tags: Int J Biochem Cell Biol Source Type: research

miR-182 contributes to cell proliferation, invasion and tumor growth in colorectal cancer by targeting DAB2IP.
Abstract miR-182 was revealed to be upregulated in colorectal cancer (CRC) and contributed to CRC development. However, the detailed molecular mechanism of miR-182 in the progression of CRC remains largely elusive. Herein, miR-182 was upregulated in CRC serum samples, CRC tissues and cells. miR-182 expression was evidently reduced in postoperative serum samples, compared with preoperative serum samples, whereas miR-182 expression was re-elevated in serum samples from CRC patients who developed postoperative recurrence. Exogenous miR-182 promoted the proliferation, colony formation, increased ki67 level and facilit...
Source: The International Journal of Biochemistry and Cell Biology - April 8, 2019 Category: Biochemistry Authors: Li X, Zhang X, Zhang Q, Lin R Tags: Int J Biochem Cell Biol Source Type: research

Corrigendum to "The landscape of chimeric RNAs in bladder urothelial carcinoma" [Int. J. Biochem. Cell Biol. 110 (February) (2019) 50-58, Epub ahead of print].
Corrigendum to "The landscape of chimeric RNAs in bladder urothelial carcinoma" [Int. J. Biochem. Cell Biol. 110 (February) (2019) 50-58, Epub ahead of print]. Int J Biochem Cell Biol. 2019 Apr 06;111:11 Authors: Zhu D, Singh S, Chen X, Zheng Z, Huang J, Lin T, Li H PMID: 30965217 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - April 6, 2019 Category: Biochemistry Authors: Zhu D, Singh S, Chen X, Zheng Z, Huang J, Lin T, Li H Tags: Int J Biochem Cell Biol Source Type: research

Identification of chimeric RNAs in human infant brains and their implications in neural differentiation.
Abstract Chimeric RNAs are transcripts composed of RNA fragments from different genes and are traditionally well-known cancer-causing genetic events. Recent studies show chimeric RNAs being present in multiple non-neoplastic tissues and cells, suggesting that at least some may have roles in normal physiology. However, chimeric RNAs and their implications in brain development and neural differentiation have not been formally studied. Here, we firstly characterized the landscape of chimeric RNAs in human infant brain tissues and identified 599 chimeric RNAs. Through a series of filtering, 22 were selected and tested...
Source: The International Journal of Biochemistry and Cell Biology - April 5, 2019 Category: Biochemistry Authors: Tang Y, Ma S, Wang X, Xing Q, Huang T, Liu H, Li Q, Zhang Y, Zhang K, MinghaoYao, Yang GL, Li H, Zang X, Yang B, Guan F Tags: Int J Biochem Cell Biol Source Type: research

MiR-9 promotes synovial sarcoma cell migration and invasion by directly targeting CDH1.
CONCLUSION: MiR-9 induces EMT by targeting CDH1, and activates MAPK/ERK and Wnt/β-catenin signal pathways, thus promoting HSS tumorigenesis. PMID: 30959202 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - April 5, 2019 Category: Biochemistry Authors: Xue-Zheng X, Li XA, Yi L, Liu JF, Hong-Wei W, Huang G Tags: Int J Biochem Cell Biol Source Type: research

Autophagy: supporting cellular and organismal homeostasis by self-eating.
Abstract Autophagy is a conserved catabolic process that delivers cytoplasmic components and organelles to lysosomes for degradation and recycling. This pathway serves to degrade nonfunctional organelles and aggregate-prone proteins, as well as to produce substrates for energy production and biosynthesis. Autophagy is especially important for the maintenance of stem cells, and for the survival and homeostasis of post-mitotic cells like neurons. Functional autophagy promotes longevity in several model organisms. Autophagy regulates immunity and inflammation at several levels and has both anti- and pro-tumorigenic r...
Source: The International Journal of Biochemistry and Cell Biology - March 30, 2019 Category: Biochemistry Authors: Eskelinen EL Tags: Int J Biochem Cell Biol Source Type: research

Corrigendum to "Induction of phosphatase shatterproof 2 by evodiamine suppresses the proliferation and invasion of human cholangiocarcinoma" [Int. J. Biochem. Cell Biol. 108 (2019) 98-110].
Corrigendum to "Induction of phosphatase shatterproof 2 by evodiamine suppresses the proliferation and invasion of human cholangiocarcinoma" [Int. J. Biochem. Cell Biol. 108 (2019) 98-110]. Int J Biochem Cell Biol. 2019 Mar 22;: Authors: Zhu B, Zhao L, Liu Y, Jin Y, Feng J, Zhao F, Sun J, Geng R, Wei Y PMID: 30910281 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - March 22, 2019 Category: Biochemistry Authors: Zhu B, Zhao L, Liu Y, Jin Y, Feng J, Zhao F, Sun J, Geng R, Wei Y Tags: Int J Biochem Cell Biol Source Type: research

Antisense oligonucleotide therapies for Amyotrophic Lateral Sclerosis: Existing and emerging targets.
Abstract Amyotrophic lateral sclerosis (ALS) is a disease with highly heterogenous causes, most of which remain unknown, a multitude of possible disease mechanisms, and no therapy currently available that can halt disease progression. However, recent advances in antisense oligonucleotides have made them a viable option for targeted therapeutics for patients. These molecules offer a method of targeting RNA that is highly specific, adaptable, and does not require viral delivery. Antisense oligonucleotides are therefore being developed for several genetic causes of ALS. Furthermore, biological pathways involved in th...
Source: The International Journal of Biochemistry and Cell Biology - March 20, 2019 Category: Biochemistry Authors: Klim JR, Vance C, Scotter EL Tags: Int J Biochem Cell Biol Source Type: research

Controlling proteolysis of Clp peptidase: a possible target for combating mitochondrial diseases.
Abstract Some mechanisms of cellular stress, aging, and apoptosis are related to proteolysis. With respect to ClpP, little is known about the mechanical manner in which the substrate is hydrolyzed in and released from the degradation chamber. Furthermore, what would be the real influence of ClpP in mammalian UPRmt? PMID: 30885675 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - March 15, 2019 Category: Biochemistry Authors: da Silva RR Tags: Int J Biochem Cell Biol Source Type: research

Fob1p recruits DNA topoisomerase I to ribosomal genes locus and contributes to its transcriptional silencing maintenance.
Abstract S. cerevisiae ribosomal DNA (rDNA) locus hosts a series of highly complex regulatory machineries for RNA polymerase I, II and III transcription, DNA replication and units recombination, all acting in the Non Transcribed Spacers (NTSs) interposed between the repeated units by which it is composed. DNA topoisomerase I (Top1p) contributes, recruiting Sir2p, to the maintenance of transcriptional silencing occurring at the RNA Polymerase II cryptic promoters, located in the NTS region. In this paper we found that Fob1p presence is crucial for Top1p recruitment at NTS, allowing transcriptional silencing to be e...
Source: The International Journal of Biochemistry and Cell Biology - March 14, 2019 Category: Biochemistry Authors: Di Felice F, Egidi A, D'Alfonso A, Camilloni G Tags: Int J Biochem Cell Biol Source Type: research

Glutamate affects the CYP1B1- and CYP2U1-mediated hydroxylation of arachidonic acid metabolism via astrocytic mGlu5 receptor.
Abstract The extrahepatic CYP enzymes, CYP1B1 and CYP2U1, have been predominantly found in both astrocytes and brain microvessels. We investigated the alteration in the production of hydroxyeicosatetraenoic acids (HETEs) from arachidonic acid (AA) mainly via CYP1B1 and CYP2U1 by glutamate. CYP1B1 and CYP2U1 mRNA levels were dose-dependently induced by glutamate in human U251 glioma cells and hCMEC/D3 blood-brain barrier cells. The increases in the CYP1B1 and CYP2U1 mRNA levels and the binding of CREB to CYP1B1 and CYP2U1 promoters following glutamate treatment were attenuated by mGlu5 receptor antagonist. The mRNA...
Source: The International Journal of Biochemistry and Cell Biology - March 8, 2019 Category: Biochemistry Authors: Yu X, Wu J, Hu M, Wu J, Zhu Q, Yang Z, Xie X, Feng YQ, Yue J Tags: Int J Biochem Cell Biol Source Type: research

The natural antisense transcript NATTD regulates the transcription of decapping scavenger (DcpS) enzyme.
In this study, we identified a novel NAT, NATTD, which is partially complementary to both the TIRAP/Mal and DcpS genes. Interestingly, NATTD only positively regulates the expression of DcpS, a decapping scavenger enzyme which is a promising therapeutic target for spinal muscular atrophy. But it has no obvious effects on the expression of TIRAP/Mal gene. The NATTD transcript primarily resides in the nucleus and does not alter the mRNA stability of DcpS. Instead, it is required for the recruitment of RNA polymerase II at the mouse DcpS promoter. Chromatin immunoprecipitation assays revealed that knocking-down NATTD transcrip...
Source: The International Journal of Biochemistry and Cell Biology - March 8, 2019 Category: Biochemistry Authors: Mei ZZ, Sun H, Ou X, Li L, Cai J, Hu S, Wang J, Luo H, Liu J, Jiang Y Tags: Int J Biochem Cell Biol Source Type: research

LPA1 receptor activation induces PKC α nuclear translocation in Glioblastoma cells.
In this study, we aimed to determine if a classical PKC (α isozyme), could be activated through LPA1 in GBM cell lines and if this activation impacts on cell number. We found that LPA1 induces PKCα translocation to the nucleus, but not to the cell membrane after LPA treatment and the cell number diminished when LPA1/PKCα signaling was blocked, suggesting a relevant role of LPA1 and PKCα in GBM growth. PMID: 30849522 [PubMed - as supplied by publisher] (Source: The International Journal of Biochemistry and Cell Biology)
Source: The International Journal of Biochemistry and Cell Biology - March 5, 2019 Category: Biochemistry Authors: Valdés-Rives SA, de la Fuente-Granada M, Velasco-Velázquez MA, González-Flores O, González-Arenas A Tags: Int J Biochem Cell Biol Source Type: research

Kinetics Mechanism and Regulation of Native Human Hepatic Thymidine Phosphorylase.
This study describes the kinetic mechanism and regulation of native human hepatic TP. The liver is a main site of pyrimidine metabolism and contains high levels of TP. Initial velocity and product inhibition studies demonstrated that the basic mechanism of this enzyme is a sequential random bi-bi mechanism. Initial velocity studies showed an intersecting pattern, consistent with substrate-enzyme-co-substrate complex formation, and a binding pattern indicating that the binding of the substrate interferes with the binding of the co-substrate and vice versa. Estimated kinetic parameters were KThymidine = 284&thi...
Source: The International Journal of Biochemistry and Cell Biology - March 5, 2019 Category: Biochemistry Authors: Oh T, El Kouni MH Tags: Int J Biochem Cell Biol Source Type: research

Loss of human arylamine N-acetyltransferase I regulates mitochondrial function by inhibition of the pyruvate dehydrogenase complex.
In this study, the effects of NAT1 deletion on mitochondrial function was examined in 2 human cell lines, breast carcinoma MDA-MB-231 and colon carcinoma HT-29 cells. Using a Seahorse XFe96 Flux Analyzer, NAT1 deletion was shown to decrease oxidative phosphorylation with a significant loss in respiratory reserve capacity in both cell lines. There also was a decrease in glycolysis without a change in glucose uptake. The changes in mitochondrial function was due to a decrease in pyruvate dehydrogenase activity, which could be reversed with the pyruvate dehydrogenase kinase inhibitor dichloroacetate. In the MDA-MB-231 and HT-...
Source: The International Journal of Biochemistry and Cell Biology - March 2, 2019 Category: Biochemistry Authors: Wang L, Minchin RF, Essebier PJ, Butcher NJ Tags: Int J Biochem Cell Biol Source Type: research

STAT3beta, a distinct isoform from STAT3.
Abstract STAT3β is an isoform of STAT3 (signal transducer and activator of transcription 3) that differs from the STAT3α isoform by the replacement of the C-terminal 55 amino acid residues with 7 specific residues. The constitutive activation of STAT3α plays a pivotal role in the activation of oncogenic pathways, such as cell proliferation, maturation and survival, while STAT3β is often referred to as a dominant-negative regulator of cancer. STAT3β reveals a "spongy cushion" effect through its cooperation with STAT3α or forms a ternary complex with other co-activators. Es...
Source: The International Journal of Biochemistry and Cell Biology - February 26, 2019 Category: Biochemistry Authors: Zhang HX, Yang PL, Li EM, Xu LY Tags: Int J Biochem Cell Biol Source Type: research

Conserved functions of RNA-binding proteins in muscle.
We present a cross-species view summarizing what is known about RNA-binding protein function in muscle, from worms and flies to zebrafish, mice and humans. In particular, we focus on alternative splicing regulated by the CELF, MBNL and RBFOX families of proteins. We discuss the systemic nature of diseases associated with loss of RNA-binding proteins in muscle, focusing on mis-regulation of CELF and MBNL in myotonic dystrophy. These examples illustrate the conservation of RNA-binding protein function and the marked utility of genetic model systems in understanding mechanisms of RNA regulation. PMID: 30818081 [PubMed - ...
Source: The International Journal of Biochemistry and Cell Biology - February 25, 2019 Category: Biochemistry Authors: Nikonova E, Kao SY, Ravichandran K, Wittner A, Spletter ML Tags: Int J Biochem Cell Biol Source Type: research

The Landscape of Chimeric RNAs in Bladder Urothelial Carcinoma.
Abstract BACKGROUND: Gene fusions and products have been identified as oncogenic drivers in many cancers, making them attractive diagnostic markers and therapeutic targets. However, the landscape of fusion transcripts in bladder cancer has not been fully characterized. METHODS: To identify fusion transcripts with potential therapeutic or diagnostic values, TCGA bladder urothelial carcinoma RNA-sequencing dataset was used. In order to avoid false positives, we applied multiple criteria including filtering out fusions detected in normal samples from GTEx dataset. We validated a subset of candidate fusions with ...
Source: The International Journal of Biochemistry and Cell Biology - February 25, 2019 Category: Biochemistry Authors: Zhu D, Singh S, Chen X, Zheng Z, Huang J, Lin T, Li H Tags: Int J Biochem Cell Biol Source Type: research

Targeting cancer stem cells as therapeutic approach in the treatment of colorectal cancer.
Abstract Colorectal cancer is one of the most common cancers globally. A large portion of colorectal cancer patients who are treated with conventional chemotherapy eventually develop local recurrence or metastases. The failure of a complete cure in colorectal cancer patients may be related to the lack of complete eradication of cancer stem cells when using conventional therapy. Colorectal cancer stem cells comprise a small population of tumor cells that possess the properties of rapid proliferation and differentiation. The colorectal cancer stem cells are also phenotypically and molecularly distinct, and resistant...
Source: The International Journal of Biochemistry and Cell Biology - February 25, 2019 Category: Biochemistry Authors: Parizadeh SM, Jafarzadeh-Esfehani R, Hassanian SM, Parizadeh SMR, Vojdani S, Ghandehari M, Ghazaghi A, Khazaei M, Shahidsales S, Rezayi M, Asgharzadeh F, Ghayour-Mobarhan M, Ferns GA, Avan A Tags: Int J Biochem Cell Biol Source Type: research

Endocytic iron trafficking and mitochondria in Parkinson's disease.
Abstract Parkinson's disease (PD) - the second most common neurodegenerative disorder - is a multifactorial disease, the causes of which should be sought in complex and detrimental interactions between genetic and environmental factors. Multiple lines of evidence, however, identify mitochondrial dysfunction, oxidative stress, and iron accumulation as central pathogenic mechanisms. These factors are closely intertwined because mitochondria are a major source of pro-oxidant species and are the major intracellular recipients of iron. How iron is transported to mitochondria, however, is largely unknown. Some studies s...
Source: The International Journal of Biochemistry and Cell Biology - February 25, 2019 Category: Biochemistry Authors: Cerri S, Milanese C, Mastroberardino PG Tags: Int J Biochem Cell Biol Source Type: research

Anti-cancer effect of Indanone-based thiazolyl hydrazone derivative on colon cancer cell lines.
Abstract Colorectal cancer is the third leading cause of cancer related deaths in the United States. Currently, Irinotecan, a topoisomerase I inhibitor, is an approved anti-cancer drug for the treatment of patients with advanced or recurrent colorectal cancer. Considering low response rate and events of high toxicity caused by irinotecan, we evaluated a series of thirteen thiazolyl hydrazone derivatives of 1-indanone for their potential antineoplastic activity and four compounds showed promising anti-cancer activity against most of the tested colon cancer cell lines with IC50 values ranging from 0.41 &plusm...
Source: The International Journal of Biochemistry and Cell Biology - February 19, 2019 Category: Biochemistry Authors: Narayanan S, Gupta P, Nazim U, Ali M, Karadkhelkar N, Ahmad M, Chen ZS Tags: Int J Biochem Cell Biol Source Type: research