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2.5D Traction Force Microscopy: Imaging three-dimensional cell forces at interfaces and biological applications
Int J Biochem Cell Biol. 2023 Jun 6:106432. doi: 10.1016/j.biocel.2023.106432. Online ahead of print.ABSTRACTThe forces that cells, tissues, and organisms exert on the surface of a soft substrate can be measured using Traction Force Microscopy (TFM), an important and well-established technique in Mechanobiology. The usual TFM technique (two-dimensional, 2D TFM) treats only the in-plane component of the traction forces and omits the out-of-plane forces at the substrate interfaces (2.5D) that turn out to be important in many biological processes such as tissue migration and tumour invasion. Here, we review the imaging, mater...
Source: The International Journal of Biochemistry and Cell Biology - June 8, 2023 Category: Biochemistry Authors: H élène Delanoë-Ayari Tetsuya Hiraiwa Philippe Marcq Jean-Paul Rieu Thuan Beng Saw Source Type: research

2.5D Traction Force Microscopy: Imaging three-dimensional cell forces at interfaces and biological applications
Int J Biochem Cell Biol. 2023 Jun 6:106432. doi: 10.1016/j.biocel.2023.106432. Online ahead of print.ABSTRACTThe forces that cells, tissues, and organisms exert on the surface of a soft substrate can be measured using Traction Force Microscopy (TFM), an important and well-established technique in Mechanobiology. The usual TFM technique (two-dimensional, 2D TFM) treats only the in-plane component of the traction forces and omits the out-of-plane forces at the substrate interfaces (2.5D) that turn out to be important in many biological processes such as tissue migration and tumour invasion. Here, we review the imaging, mater...
Source: The International Journal of Biochemistry and Cell Biology - June 8, 2023 Category: Biochemistry Authors: H élène Delanoë-Ayari Tetsuya Hiraiwa Philippe Marcq Jean-Paul Rieu Thuan Beng Saw Source Type: research

2.5D Traction Force Microscopy: Imaging three-dimensional cell forces at interfaces and biological applications
Int J Biochem Cell Biol. 2023 Jun 6:106432. doi: 10.1016/j.biocel.2023.106432. Online ahead of print.ABSTRACTThe forces that cells, tissues, and organisms exert on the surface of a soft substrate can be measured using Traction Force Microscopy (TFM), an important and well-established technique in Mechanobiology. The usual TFM technique (two-dimensional, 2D TFM) treats only the in-plane component of the traction forces and omits the out-of-plane forces at the substrate interfaces (2.5D) that turn out to be important in many biological processes such as tissue migration and tumour invasion. Here, we review the imaging, mater...
Source: The International Journal of Biochemistry and Cell Biology - June 8, 2023 Category: Biochemistry Authors: H élène Delanoë-Ayari Tetsuya Hiraiwa Philippe Marcq Jean-Paul Rieu Thuan Beng Saw Source Type: research

2.5D Traction Force Microscopy: Imaging three-dimensional cell forces at interfaces and biological applications
Int J Biochem Cell Biol. 2023 Jun 6:106432. doi: 10.1016/j.biocel.2023.106432. Online ahead of print.ABSTRACTThe forces that cells, tissues, and organisms exert on the surface of a soft substrate can be measured using Traction Force Microscopy (TFM), an important and well-established technique in Mechanobiology. The usual TFM technique (two-dimensional, 2D TFM) treats only the in-plane component of the traction forces and omits the out-of-plane forces at the substrate interfaces (2.5D) that turn out to be important in many biological processes such as tissue migration and tumour invasion. Here, we review the imaging, mater...
Source: The International Journal of Biochemistry and Cell Biology - June 8, 2023 Category: Biochemistry Authors: H élène Delanoë-Ayari Tetsuya Hiraiwa Philippe Marcq Jean-Paul Rieu Thuan Beng Saw Source Type: research

Mitochondria are midfield players in steroid synthesis
Int J Biochem Cell Biol. 2023 May 18;160:106431. doi: 10.1016/j.biocel.2023.106431. Online ahead of print.ABSTRACTSteroids are important membrane components and signaling metabolites and thus are required for cellular homeostasis. All mammalian cells retain the ability to uptake and synthesize steroids. Dysregulation of steroid levels leads to profound effects on cellular function and organismal health. Hence it comes as no surprise that steroid synthesis is tightly regulated. It is well established that the main site for steroid synthesis and regulation is the endoplasmic reticulum. However, mitochondria are essential for...
Source: The International Journal of Biochemistry and Cell Biology - May 19, 2023 Category: Biochemistry Authors: Philipp Melchinger Bruna Martins Garcia Source Type: research