The International Journal of Biochemistry and Cell Biology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Using Single Molecule Imaging to Explore Intracellular Heterogeneity
Int J Biochem Cell Biol. 2023 Aug 14:106455. doi: 10.1016/j.biocel.2023.106455. Online ahead of print.ABSTRACTDespite more than 100 years of study, it is unclear if the movement of proteins inside the cell is best described as a mosh pit or an exquisitely choreographed dance. Recent studies suggest the latter. Local interactions induce molecular condensates such as liquid-liquid phase separations (LLPSs) or non-liquid, functionally significant molecular aggregates, including synaptic densities, nucleoli, and Amyloid fibrils. Molecular condensates trigger intracellular signaling and drive processes ranging from gene express...
Source: The International Journal of Biochemistry and Cell Biology - August 16, 2023 Category: Biochemistry Authors: James A Galbraith Catherine G Galbraith Source Type: research

Circadian control of cisplatin-DNA adduct repair and apoptosis in culture cells
We examined the DNA damage responses in circadian-synchronized wild-type mouse embryonic fibroblasts (WT-MEF; clock-proficient cells), cryptochrome1 and 2 double knock-out MEF (CRYDKO; clock-deficient cells), and mouse hepatocarcinoma Hepa1c1c7 cells. Varying the treatment time resulted in a significant difference in the rate of platinum-DNA adduct removal specifically in circadian-synchronized WT-MEF, while CRYDKO did not exhibit such variation. Moreover, diurnal variation in other DNA damage responses, such as cell cycle checkpoint activity indicated by p53 phosphorylation status and apoptosis measured by DNA break frequ...
Source: The International Journal of Biochemistry and Cell Biology - August 13, 2023 Category: Biochemistry Authors: Jeseok Jeon Sanggon Lee Jeong-Min Park Tae-Hee Lee Tae-Hong Kang Source Type: research

Circadian Control of Cisplatin-DNA Adduct Repair and Apoptosis in Culture Cells
We examined the DNA damage responses in circadian-synchronized wild-type mouse embryonic fibroblasts (WT-MEF; clock-proficient cells), cryptochrome1 and 2 double knock-out MEF (CRYDKO; clock-deficient cells), and mouse hepatocarcinoma Hepa1c1c7 cells. Varying the treatment time resulted in a significant difference in the rate of platinum-DNA adduct removal specifically in circadian-synchronized WT-MEF, while CRYDKO did not exhibit such variation. Moreover, diurnal variation in other DNA damage responses, such as cell cycle checkpoint activity indicated by p53 phosphorylation status and apoptosis measured by DNA break frequ...
Source: The International Journal of Biochemistry and Cell Biology - August 13, 2023 Category: Biochemistry Authors: Jeseok Jeon Sanggon Lee Jeong-Min Park Tae-Hee Lee Tae-Hong Kang Source Type: research

Cancer: Phosphodiesterase type 4C (PDE4C), the forgotten subfamily as a therapeutic target
Int J Biochem Cell Biol. 2023 Jul 25;162:106453. doi: 10.1016/j.biocel.2023.106453. Online ahead of print.ABSTRACTPhosphodiesterase type 4 (PDE4) enzymes specifically hydrolyse cAMP in many cell signalling systems that are transduced by hormones and other primary messengers. The physiological function of the four PDE4 subfamilies (A, B, C and D) are numerous and varied due to the differentially localised plethora of isoforms that can be detected in cardiovascular, CNS and immune systems. Of the four subfamilies, least is known about PDE4C probably due to its restricted distribution pattern, scarcity of selective inhibitors...
Source: The International Journal of Biochemistry and Cell Biology - July 27, 2023 Category: Biochemistry Authors: Thomas A Wright Alistair O Gemmell Gonzalo S Tejeda Connor M Blair George S Baillie Source Type: research