Antimicrobial resistance and carbapenemase dissemination in < em > Pseudomonas aeruginosa < /em > isolates from Libyan hospitals: a call for surveillance and intervention
This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolates, and the microorganisms' capacity for alginate and biofilm production. Forty-five isolates were collected from four hospitals in Benghazi and Derna, Libya. Antimicrobial susceptibility was determined using agar disc diffusion. The presence of resistance genes (blaCTXM, blaTEM, blaSHV-1, blaGES-1, blaKPC, and blaNDM) was screened using PCR. Biofilm formation was quantified via the crystal violet assay, while al...
Source: Libyan Journal of Medicine - April 21, 2024 Category: General Medicine Authors: Nasrin K Gadaime Randa N Haddadin Asem A Shehabi Intisar N Omran Source Type: research
KPC-2-producing Enterobacterales from ready-to-eat food to hospitalized patients
Infect Genet Evol. 2024 Apr 19:105596. doi: 10.1016/j.meegid.2024.105596. Online ahead of print.ABSTRACTFoodstuffs are a well-documented source of multidrug-resistant bacteria, and hospitalized patients are usually susceptible to hospital infections owing to their immune status. Therefore, this study aimed to investigate the presence of beta-lactamase-producing Enterobacterales in ready-to-eat foods consumed by hospitalized patients. For this purpose, 51 vegetable and meat samples were collected over 2 months and analyzed. Enterobacterales isolates were identified and subjected to antimicrobial susceptibility testing, foll...
Source: Infection, Genetics and Evolution - April 21, 2024 Category: Genetics & Stem Cells Authors: Bianca Lara Ven âncio de Godoy Marlon do Valle Barroso Yasmin Fontoura de Azeredo Louren ção Let ícia Kellen de Andrade Vit ória Gabriela Tosta Rodrigues Caroline Atu í Ana Caroline do Valle Ta ís Paulino Ferreira Mara Corr êa Lelles Nogueira Tiag Source Type: research
Antimicrobial resistance and carbapenemase dissemination in < em > Pseudomonas aeruginosa < /em > isolates from Libyan hospitals: a call for surveillance and intervention
This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolates, and the microorganisms' capacity for alginate and biofilm production. Forty-five isolates were collected from four hospitals in Benghazi and Derna, Libya. Antimicrobial susceptibility was determined using agar disc diffusion. The presence of resistance genes (blaCTXM, blaTEM, blaSHV-1, blaGES-1, blaKPC, and blaNDM) was screened using PCR. Biofilm formation was quantified via the crystal violet assay, while al...
Source: Libyan Journal of Medicine - April 21, 2024 Category: General Medicine Authors: Nasrin K Gadaime Randa N Haddadin Asem A Shehabi Intisar N Omran Source Type: research
Chemo-photothermal therapy of chitosan/gold nanorod clusters for antibacterial treatment against the infection of planktonic and biofilm MRSA
In this study, we developed chemo-photothermal therapy (chemo-PTT) based on near-infrared (NIR)-irradiated chitosan/gold nanorod (GNR) clusters as anti-methicillin-resistant Staphylococcus aureus (MRSA) agents. The nanocomposites exhibited an average size of 223 nm with a surface charge of 36 mV. These plasmonic nanocomposites demonstrated on-demand and rapid hyperthermal action under NIR. The combined effect of positive charge and PTT by NIR-irradiated nanocomposites resulted in a remarkable inhibition rate of 96 % against planktonic MRSA, indicating a synergistic activity compared to chitosan nanoparticles or GNR alone. ...
Source: International Journal of Biological Macromolecules - April 20, 2024 Category: Biochemistry Authors: G R Nirmal Zih-Chan Lin Tai-Sheng Chiu Ahmed Alalaiwe Chia-Chih Liao Jia-You Fang Source Type: research
Evaluation of Moringa Oleifera Leaf Extract for its In vitro Antibacterial Properties, Mechanism of Action, and In vivo Corneal Ulcer Healing Effects in Rabbits' Eyes
Curr Drug Deliv. 2024 Apr 17. doi: 10.2174/0115672018275561240228065755. Online ahead of print.ABSTRACTM. oleifera is the most adapted tree species in different medicinal eco-systems and has resilience against climate changes. This multiple-use tree provides healthy foods, snacks, honey, and fuel. Besides this, it has immense promising applicationsby offering antimicrobial and antibacterial activities for targeted uses. This validates the court of Hippocrates that let food be the medicine and medicine be the food for which moringa qualifies. In view of this, the antioxidant and in vitro antibacterial potency of the hydro-e...
Source: Current Drug Delivery - April 19, 2024 Category: Drugs & Pharmacology Authors: Ayesha Bibi Meenakshi Dhanawat Shahbaz Aman Samrat Chauhan Rishabh Chalotra Somdutt Mujwar Narinder Kaur ChamasseHomary Maivagna Sumeet Gupta Source Type: research
Managing Antimicrobial Resistance in the Emergency Department
Emerg Med Clin North Am. 2024 May;42(2):461-483. doi: 10.1016/j.emc.2024.02.005. Epub 2024 Mar 15.ABSTRACT(Basic awareness and understanding of antimicrobial resistance and prevailing mechanisms can aid emergency physicians in providing appropriate care to patients with infections due to a multidrug-resistant organism (MDRO). Empiric treatment of MDRO infections should be approached with caution and guided by the most likely pathogens based on differential diagnosis, severity of the illness, suspected source of infection, patient-specific factors, and local antibiotic susceptibility patterns. Newer broad-spectrum antibioti...
Source: The Medical Clinics of North America - April 19, 2024 Category: General Medicine Authors: Julianne Yeary Larissa Hacker Stephen Y Liang Source Type: research
First vertebrate BRICHOS antimicrobial peptides: β-hairpin host defense peptides in limbless amphibia lung resemble those of marine worms
Biochem Biophys Res Commun. 2024 Apr 10;712-713:149913. doi: 10.1016/j.bbrc.2024.149913. Online ahead of print.ABSTRACTInnate immunity of invertebrates offers potent antimicrobial peptides (AMPs) against drug-resistant infections. To identify new worm β-hairpin AMPs, we explored the sequence diversity of proteins with a BRICHOS domain, which comprises worm AMP precursors. Strikingly, we discovered new BRICHOS AMPs not in worms, but in caecilians, the least studied clade of vertebrates. Two precursor proteins from Microcaecilia unicolor and Rhinatrema bivittatum resemble SP-C lung surfactants and bear worm AMP-like peptide...
Source: Biochemical and Biophysical Research communications - April 19, 2024 Category: Biochemistry Authors: Ruslan V Smetanin Maria S Sukhareva Elizaveta V Vladimirova Maria S Zharkova Anna D Mikushina Aleksey S Komlev Maria M Khaydukova Tatiana A Filatenkova Anastasia I Kalganova Sofiya O Pipiya Stanislav S Terekhov Dmitriy S Orlov Olga V Shamova Igor E Elisee Source Type: research
Novel hydrazone compounds with broad-spectrum antiplasmodial activity and synergistic interactions with antimalarial drugs
This study evaluated the antiplasmodial activity of seven novel hydrazone compounds (referred to as CB compounds: CB-27, CB-41, CB-50, CB-53, CB-58, CB-59, and CB-61) against multiple stages of Plasmodium parasites. All CB compounds inhibited blood stage proliferation of drug-resistant or sensitive strains of Plasmodium falciparum in the low micromolar to nanomolar range. Interestingly, CB-41 exhibited prophylactic activity against hypnozoites and liver schizonts in Plasmodium cynomolgi, a primate model for Plasmodium vivax. Four CB compounds (CB-27, CB-41, CB-53, and CB-61) inhibited P. falciparum oocyst formation in mosq...
Source: Antimicrobial Agents and Chemotherapy - April 19, 2024 Category: Microbiology Authors: Ang élica M Rosado-Quiñones Emilee E Col ón-Lorenzo Zarna Rajeshkumar Pala J ürgen Bosch Karl Kudyba Heather Kudyba Susan E Leed Norma Roncal Abel Baerga-Ortiz Abiel Roche-Lima Yamil Gerena David A Fidock Alison Roth Joel Vega-Rodr íguez Adelfa E Ser Source Type: research
Evaluation of Moringa Oleifera Leaf Extract for its In vitro Antibacterial Properties, Mechanism of Action, and In vivo Corneal Ulcer Healing Effects in Rabbits' Eyes
Curr Drug Deliv. 2024 Apr 17. doi: 10.2174/0115672018275561240228065755. Online ahead of print.ABSTRACTM. oleifera is the most adapted tree species in different medicinal eco-systems and has resilience against climate changes. This multiple-use tree provides healthy foods, snacks, honey, and fuel. Besides this, it has immense promising applicationsby offering antimicrobial and antibacterial activities for targeted uses. This validates the court of Hippocrates that let food be the medicine and medicine be the food for which moringa qualifies. In view of this, the antioxidant and in vitro antibacterial potency of the hydro-e...
Source: Current Drug Delivery - April 19, 2024 Category: Drugs & Pharmacology Authors: Ayesha Bibi Meenakshi Dhanawat Shahbaz Aman Samrat Chauhan Rishabh Chalotra Somdutt Mujwar Narinder Kaur ChamasseHomary Maivagna Sumeet Gupta Source Type: research
Managing Antimicrobial Resistance in the Emergency Department
Emerg Med Clin North Am. 2024 May;42(2):461-483. doi: 10.1016/j.emc.2024.02.005. Epub 2024 Mar 15.ABSTRACT(Basic awareness and understanding of antimicrobial resistance and prevailing mechanisms can aid emergency physicians in providing appropriate care to patients with infections due to a multidrug-resistant organism (MDRO). Empiric treatment of MDRO infections should be approached with caution and guided by the most likely pathogens based on differential diagnosis, severity of the illness, suspected source of infection, patient-specific factors, and local antibiotic susceptibility patterns. Newer broad-spectrum antibioti...
Source: The Medical Clinics of North America - April 19, 2024 Category: General Medicine Authors: Julianne Yeary Larissa Hacker Stephen Y Liang Source Type: research
