Circulating miRNAs: Potential diagnostic role for coronavirus disease 2019 (COVID-19)
In this study, we compared the signature of circulating miRNAs in plasma of COVID-19 patients versus healthy donors. MiRCURY LNA miRNA miRNome qPCR Panels were performed for miRNA signature characterization. Individual quantitative real-time PCR (qRT-PCR) was carried out to validate miRNome qPCR results. Receiver-operator characteristic (ROC) curve analysis was applied to assess the diagnostic accuracy of the most significantly deregulated miRNA(s) as potential diagnostic biomarker(s). Eight miRNAs were identified to be differentially expressed with miR-17-5p and miR-142-5p being down-regulated whilst miR-15a-5p, miR-19a-3...
Source: Infection, Genetics and Evolution - August 3, 2021 Category: Genetics & Stem Cells Authors: Mohammad Fayyad-Kazan Rawan Makki Najwa Skafi Mahmoud El Homsi Aline Hamade Rania El Majzoub Eva Hamade Hussein Fayyad-Kazan Bassam Badran Source Type: research

Mycobacterium tuberculosis RKIP (Rv2140c) dephosphorylates ERK/NF- κB upstream signaling molecules to subvert macrophage innate immune response
In this report, we show that Rv2140c can mimic the mammalian RKIP function. Rv2140c inhibit the activation of extracellular signal-regulated kinase (ERK) and nuclear factor κB (NF-κB) via decreasing the phosphorylation capacity of upstream mediators MEK1, ERK1/2, and IKKα/β, thus leading to a reduction in pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. This effect can be reversed by RKIP inhibitor locostatin. Furthermore Rv2140c mediates apoptosis associated with activation of caspases cascades. This modulation enhances the intracellular survival of M. smegmatis within macrophage. We pr...
Source: Infection, Genetics and Evolution - August 1, 2021 Category: Genetics & Stem Cells Authors: M A Abo-Kadoum Mohammed Assad Moure Uae Stech A E Nzaou Zhen Gong Asmaa Moaaz Samson Teweldebrhan Adel Eltoukhy Ai Xuefeng Yu Chen Jianping Xie Source Type: research

Mycobacterium tuberculosis RKIP (Rv2140c) dephosphorylates ERK/NF- κB upstream signaling molecules to subvert macrophage innate immune response
In this report, we show that Rv2140c can mimic the mammalian RKIP function. Rv2140c inhibit the activation of extracellular signal-regulated kinase (ERK) and nuclear factor κB (NF-κB) via decreasing the phosphorylation capacity of upstream mediators MEK1, ERK1/2, and IKKα/β, thus leading to a reduction in pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. This effect can be reversed by RKIP inhibitor locostatin. Furthermore Rv2140c mediates apoptosis associated with activation of caspases cascades. This modulation enhances the intracellular survival of M. smegmatis within macrophage. We pr...
Source: Infection, Genetics and Evolution - August 1, 2021 Category: Genetics & Stem Cells Authors: M A Abo-Kadoum Mohammed Assad Moure Uae Stech A E Nzaou Zhen Gong Asmaa Moaaz Samson Teweldebrhan Adel Eltoukhy Ai Xuefeng Yu Chen Jianping Xie Source Type: research

Immunoinformatics analysis of antigenic epitopes and designing of a multi-epitope peptide vaccine from putative nitro-reductases of M. tuberculosis DosR
Infect Genet Evol. 2021 Jul 28:105017. doi: 10.1016/j.meegid.2021.105017. Online ahead of print.ABSTRACTMycobacterium tuberculosis resides in alveolar macrophages as a non-dividing and dormant state causing latent tuberculosis. Currently, no vaccine available against the latent tuberculosis. Latent Mtb expresses ~48 genes under the control of DosR regulon. Among these, putative nitroreductases have significantly high expression levels, help Mtb to cope up with nitrogen stresses, and possess antigenic properties. In the current study, immunoinformatics methodologies are applied to predict the promiscuous antigenic T-cell ep...
Source: Infection, Genetics and Evolution - July 31, 2021 Category: Genetics & Stem Cells Authors: Mohd Shiraz Surabhi Lata Pankaj Kumar Umate Nachiket Shankar Mohd Akif Source Type: research

Campylobacter coli isolated in Brazil typed by core genome Multilocus Sequence Typing shows high genomic diversity in a global context
CONCLUSIONS: The results showed a high similarity among some strains studied and a wide distribution of the Brazilian strains when compared to isolates from different countries, which is an interesting data set since it showed a high genetic diversity of these strains from Brazil in a global context. This study contributed for a better genomic characterization of C. coli strains isolated in Brazil and provides important information about the diversity of this clinically-relevant pathogen.PMID:34332158 | DOI:10.1016/j.meegid.2021.105018 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 31, 2021 Category: Genetics & Stem Cells Authors: Carolina Nogueira Gomes Dillon Oliver Reese Barker Sheila da Silva Duque Emily Victoria Che Vasena Jayamanna Eduardo Napoleon Taboada Juliana Pfrimer Falc ão Source Type: research

Immunoinformatics analysis of antigenic epitopes and designing of a multi-epitope peptide vaccine from putative nitro-reductases of M. tuberculosis DosR
Infect Genet Evol. 2021 Jul 28:105017. doi: 10.1016/j.meegid.2021.105017. Online ahead of print.ABSTRACTMycobacterium tuberculosis resides in alveolar macrophages as a non-dividing and dormant state causing latent tuberculosis. Currently, no vaccine available against the latent tuberculosis. Latent Mtb expresses ~48 genes under the control of DosR regulon. Among these, putative nitroreductases have significantly high expression levels, help Mtb to cope up with nitrogen stresses, and possess antigenic properties. In the current study, immunoinformatics methodologies are applied to predict the promiscuous antigenic T-cell ep...
Source: Infection, Genetics and Evolution - July 31, 2021 Category: Genetics & Stem Cells Authors: Mohd Shiraz Surabhi Lata Pankaj Kumar Umate Nachiket Shankar Mohd Akif Source Type: research

Campylobacter coli isolated in Brazil typed by core genome Multilocus Sequence Typing shows high genomic diversity in a global context
CONCLUSIONS: The results showed a high similarity among some strains studied and a wide distribution of the Brazilian strains when compared to isolates from different countries, which is an interesting data set since it showed a high genetic diversity of these strains from Brazil in a global context. This study contributed for a better genomic characterization of C. coli strains isolated in Brazil and provides important information about the diversity of this clinically-relevant pathogen.PMID:34332158 | DOI:10.1016/j.meegid.2021.105018 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 31, 2021 Category: Genetics & Stem Cells Authors: Carolina Nogueira Gomes Dillon Oliver Reese Barker Sheila da Silva Duque Emily Victoria Che Vasena Jayamanna Eduardo Napoleon Taboada Juliana Pfrimer Falc ão Source Type: research

Prevalence and genetic analysis of porcine circovirus type 2 (PCV2) and type 3 (PCV3) between 2018 and 2020 in central China
Infect Genet Evol. 2021 Jul 26:105016. doi: 10.1016/j.meegid.2021.105016. Online ahead of print.ABSTRACTPorcine circovirus type 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVAD), causing substantial economic losses to the swine industry worldwide. PCV3, as a recently discovered virus, is associated with porcine dermatitis, nephropathy syndrome, reproductive failure, congenital tremors, and other clinical symptoms. To further investigate the epidemic profile and genetic diversity of the two viruses, a total of 198 samples from swine at various growth stages suspected for PCVAD on 55 different...
Source: Infection, Genetics and Evolution - July 29, 2021 Category: Genetics & Stem Cells Authors: Tong Xu Yuan-Hang Zhang Run-Bo Tian Cheng-Yao Hou Xin-Sheng Li Lan-Lan Zheng Lin-Qing Wang Hong-Ying Chen Source Type: research

Detection and genomic characterization of mcr-9 in Enterobacter hormaechei recovered from a pediatric patient in Lebanon
CONCLUSION: The silent spread of mcr-9, particularly in the globally successful ST-78 Enterobacter lineage, is worrisome and requires close monitoring in humans and animals.PMID:34325053 | DOI:10.1016/j.meegid.2021.105014 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 29, 2021 Category: Genetics & Stem Cells Authors: Ragheed Khodor Tamara Salloum Tamima El Jisr Mira El Chaar Sima Tokajian Source Type: research

Prevalence and genetic analysis of porcine circovirus type 2 (PCV2) and type 3 (PCV3) between 2018 and 2020 in central China
Infect Genet Evol. 2021 Jul 26:105016. doi: 10.1016/j.meegid.2021.105016. Online ahead of print.ABSTRACTPorcine circovirus type 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVAD), causing substantial economic losses to the swine industry worldwide. PCV3, as a recently discovered virus, is associated with porcine dermatitis, nephropathy syndrome, reproductive failure, congenital tremors, and other clinical symptoms. To further investigate the epidemic profile and genetic diversity of the two viruses, a total of 198 samples from swine at various growth stages suspected for PCVAD on 55 different...
Source: Infection, Genetics and Evolution - July 29, 2021 Category: Genetics & Stem Cells Authors: Tong Xu Yuan-Hang Zhang Run-Bo Tian Cheng-Yao Hou Xin-Sheng Li Lan-Lan Zheng Lin-Qing Wang Hong-Ying Chen Source Type: research

Detection and genomic characterization of mcr-9 in Enterobacter hormaechei recovered from a pediatric patient in Lebanon
CONCLUSION: The silent spread of mcr-9, particularly in the globally successful ST-78 Enterobacter lineage, is worrisome and requires close monitoring in humans and animals.PMID:34325053 | DOI:10.1016/j.meegid.2021.105014 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 29, 2021 Category: Genetics & Stem Cells Authors: Ragheed Khodor Tamara Salloum Tamima El Jisr Mira El Chaar Sima Tokajian Source Type: research

Prevalence and genetic analysis of porcine circovirus type 2 (PCV2) and type 3 (PCV3) between 2018 and 2020 in central China
Infect Genet Evol. 2021 Jul 26:105016. doi: 10.1016/j.meegid.2021.105016. Online ahead of print.ABSTRACTPorcine circovirus type 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVAD), causing substantial economic losses to the swine industry worldwide. PCV3, as a recently discovered virus, is associated with porcine dermatitis, nephropathy syndrome, reproductive failure, congenital tremors, and other clinical symptoms. To further investigate the epidemic profile and genetic diversity of the two viruses, a total of 198 samples from swine at various growth stages suspected for PCVAD on 55 different...
Source: Infection, Genetics and Evolution - July 29, 2021 Category: Genetics & Stem Cells Authors: Tong Xu Yuan-Hang Zhang Run-Bo Tian Cheng-Yao Hou Xin-Sheng Li Lan-Lan Zheng Lin-Qing Wang Hong-Ying Chen Source Type: research

Detection and genomic characterization of mcr-9 in Enterobacter hormaechei recovered from a pediatric patient in Lebanon
CONCLUSION: The silent spread of mcr-9, particularly in the globally successful ST-78 Enterobacter lineage, is worrisome and requires close monitoring in humans and animals.PMID:34325053 | DOI:10.1016/j.meegid.2021.105014 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 29, 2021 Category: Genetics & Stem Cells Authors: Ragheed Khodor Tamara Salloum Tamima El Jisr Mira El Chaar Sima Tokajian Source Type: research

Mycobacterium lepromatosis MLPM_5000 is a potential heme chaperone protein HemW and mis-annotation of its orthologues in mycobacteria
Infect Genet Evol. 2021 Jul 23:105015. doi: 10.1016/j.meegid.2021.105015. Online ahead of print.ABSTRACTThe genome of a newly identified leprosy causing bacillus Mycobacterium lepromatosis was sequenced in 2015 wherein a gene MLPM_5000 was detected whose corresponding sequences are missing in its close relative Mycobacterium leprae, the well-known causal agent of leprosy. Thus MLPM_5000 is considered to be a specific genomic locus for differentiating M. lepromatosis from M. leprae. The locus was annotated as HemN (Coproporphyrinogen III oxidase) based on the available annotations in other mycobacterial species. However, we...
Source: Infection, Genetics and Evolution - July 26, 2021 Category: Genetics & Stem Cells Authors: Mukul Sharma Yash Gupta Purna Dwivedi Prakasha Kempaiah Pushpendra Singh Source Type: research

Mycobacterium lepromatosis MLPM_5000 is a potential heme chaperone protein HemW and mis-annotation of its orthologues in mycobacteria
Infect Genet Evol. 2021 Jul 23:105015. doi: 10.1016/j.meegid.2021.105015. Online ahead of print.ABSTRACTThe genome of a newly identified leprosy causing bacillus Mycobacterium lepromatosis was sequenced in 2015 wherein a gene MLPM_5000 was detected whose corresponding sequences are missing in its close relative Mycobacterium leprae, the well-known causal agent of leprosy. Thus MLPM_5000 is considered to be a specific genomic locus for differentiating M. lepromatosis from M. leprae. The locus was annotated as HemN (Coproporphyrinogen III oxidase) based on the available annotations in other mycobacterial species. However, we...
Source: Infection, Genetics and Evolution - July 26, 2021 Category: Genetics & Stem Cells Authors: Mukul Sharma Yash Gupta Purna Dwivedi Prakasha Kempaiah Pushpendra Singh Source Type: research

Mycobacterium lepromatosis MLPM_5000 is a potential heme chaperone protein HemW and mis-annotation of its orthologues in mycobacteria
Infect Genet Evol. 2021 Jul 23:105015. doi: 10.1016/j.meegid.2021.105015. Online ahead of print.ABSTRACTThe genome of a newly identified leprosy causing bacillus Mycobacterium lepromatosis was sequenced in 2015 wherein a gene MLPM_5000 was detected whose corresponding sequences are missing in its close relative Mycobacterium leprae, the well-known causal agent of leprosy. Thus MLPM_5000 is considered to be a specific genomic locus for differentiating M. lepromatosis from M. leprae. The locus was annotated as HemN (Coproporphyrinogen III oxidase) based on the available annotations in other mycobacterial species. However, we...
Source: Infection, Genetics and Evolution - July 26, 2021 Category: Genetics & Stem Cells Authors: Mukul Sharma Yash Gupta Purna Dwivedi Prakasha Kempaiah Pushpendra Singh Source Type: research

Corrigendum to Is the activity of CGRP and Adrenomedullin regulated by RAMP (-2) and (-3) in Trypanosomatidae? An in-silico approach: [Infect Genet Evol. 2018 Jul;61:197-206]
Infect Genet Evol. 2021 Jul 21:105002. doi: 10.1016/j.meegid.2021.105002. Online ahead of print.NO ABSTRACTPMID:34303608 | DOI:10.1016/j.meegid.2021.105002 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 25, 2021 Category: Genetics & Stem Cells Authors: A Febres O Vanegas M Giammarresi C Gomes E D íaz A Ponte-Sucre Source Type: research

A high-throughput sequencing shows co-infection and intermediate form of Fasciola gigantica and Fasciola hepatica in occur Balochistan, but only confirmed F. gigantica in the Punjab province of Pakistan
Infect Genet Evol. 2021 Jul 21:105012. doi: 10.1016/j.meegid.2021.105012. Online ahead of print.ABSTRACTFasciola gigantica and Fasciola hepatica are digenetic trematodes causing fasciolosis in ruminants. The host and geographical distribution of both Fasciola species are influenced by environmental and climatic conditions favouring survival and development of free-living stages and intermediate hosts, and livestock management practices. The aim of the present study was to describe the host distribution of the two Fasciola species in buffalo, cattle, goats, and sheep in the Balochistan and Punjab provinces of Pakistan. 359 ...
Source: Infection, Genetics and Evolution - July 24, 2021 Category: Genetics & Stem Cells Authors: Zia Ur Rehman Kirsty Martin Osama Zahid Qasim Ali Imran Rashid Mian Abdul Hafeez Nisar Ahmad Kamran Ashraf Martha Betson Neil D Sargison Umer Chaudhry Source Type: research

A high-throughput sequencing shows co-infection and intermediate form of Fasciola gigantica and Fasciola hepatica in occur Balochistan, but only confirmed F. gigantica in the Punjab province of Pakistan
Infect Genet Evol. 2021 Jul 21:105012. doi: 10.1016/j.meegid.2021.105012. Online ahead of print.ABSTRACTFasciola gigantica and Fasciola hepatica are digenetic trematodes causing fasciolosis in ruminants. The host and geographical distribution of both Fasciola species are influenced by environmental and climatic conditions favouring survival and development of free-living stages and intermediate hosts, and livestock management practices. The aim of the present study was to describe the host distribution of the two Fasciola species in buffalo, cattle, goats, and sheep in the Balochistan and Punjab provinces of Pakistan. 359 ...
Source: Infection, Genetics and Evolution - July 24, 2021 Category: Genetics & Stem Cells Authors: Zia Ur Rehman Kirsty Martin Osama Zahid Qasim Ali Imran Rashid Mian Abdul Hafeez Nisar Ahmad Kamran Ashraf Martha Betson Neil D Sargison Umer Chaudhry Source Type: research

Two newly isolated GVI lineage infectious bronchitis viruses in China show unique molecular and pathogenicity characteristics
Infect Genet Evol. 2021 Jul 19:105006. doi: 10.1016/j.meegid.2021.105006. Online ahead of print.ABSTRACTDuring 2016 to 2020, GVI-1 type infectious bronchitis virus (IBV) strains were sporadically reported across China, indicating a new epidemic trend of the virus. Here we investigated the molecular characteristics and pathogenicity of two newly isolated GVI-1 type IBV virus strains (CK/CH/TJ1904 and CK/CH/NP2011) from infected chicken farms in China. Genetic evolution analysis of the S1 gene showed the highest homology with the GVI-1 representative strain, TC07-2. Phylogenetic analysis and recombination analysis of the vir...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Lu Sun Xinyan Tang Jingyi Qi Chunyan Zhang Jing Zhao Guozhong Zhang Ye Zhao Source Type: research

Cloning and sequencing of lsaE efflux pump gene from MDR Enterococci and its role in erythromycin resistance
Infect Genet Evol. 2021 Jul 19:105010. doi: 10.1016/j.meegid.2021.105010. Online ahead of print.ABSTRACTEnterococci are opportunistic members of intestinal microbiota with notable ability to transmit antimicrobial resistance genes. Among the different resistance mechanisms, multidrug efflux is evolving as a huge problem in conferring multidrug resistance to bacterial cells because these pumps extrude a broad range of antimicrobials. Therefore, the aim of this work was to evaluate role of efflux pumps in the development of multi-drug resistance in Enterococci through studying the antimicrobial resistance profiles of Enteroc...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Akram N Salah Nooran S Elleboudy Ghadir S El-Housseiny Mahmoud A Yassien Source Type: research

Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India
We report here a targeted risk-based study to investigate the presence of influenza A viruses at the migratory-wild-domestic bird interface across the major wetlands of central India's Maharashtra state during the winter migration season. The H9N2 viruses have been isolated and confirmed in 3.86% (33/854) of the fecal samples of resident birds. To investigate the genetic pools of H9N2 circulating in resident birds, we sequenced two isolates of H9N2 from distant wetlands. Sequence and phylogenetic analyses have shown that these viruses are triple reassortants, with HA, NA, NP, and M genes belonging to G1 sub-lineage (A/quai...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Richa Sood Naveen Kumar Suresh S Gokhe Atul Kumar Pateriya Sushant Bhat Sandeep Bhatia Sivasankar Panickan Anamika Mishra H V Murugkar Roma Dixit Deepali Shrivastava Pushpendra Singh Meghna Tripathi Vijendra Pal Singh Source Type: research

Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998-2005): Pre-rotavirus vaccine era
Infect Genet Evol. 2021 Jul 19:105007. doi: 10.1016/j.meegid.2021.105007. Online ahead of print.ABSTRACTHuman adenovirus (HAdV) is recognized as frequent cause of acute gastroenteritis and enteric viruses can be preserved in frozen stored feces for long periods of times. The purpose of the present study was to investigate enteric HAdV genotypic diversity in archival fecal specimens stored from 1998 to 2005 in order to understand the natural history of HAdV in diarrheal patients in Brazil before rotavirus vaccine introduction. A total of 3346 specimens were tested for HAdV using conventional PCR. Genotypes were identified b...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Yasmin Fran ça Viana Pires de Souza Ellen Viana de Souza Lais Sampaio de Azevedo Roberta Salzone Medeiros Maria do Carmo Sampaio Tavares Timenetsky Adriana Luchs Source Type: research

SARS-CoV-2 as a betacoronavirus comprises five structural proteins?
Infect Genet Evol. 2021 Jul 19;94:105011. doi: 10.1016/j.meegid.2021.105011. Online ahead of print.NO ABSTRACTPMID:34293483 | PMC:PMC8289725 | DOI:10.1016/j.meegid.2021.105011 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Milad Zandi Saber Soltani Kambiz Feyzi Source Type: research

Two newly isolated GVI lineage infectious bronchitis viruses in China show unique molecular and pathogenicity characteristics
Infect Genet Evol. 2021 Jul 19:105006. doi: 10.1016/j.meegid.2021.105006. Online ahead of print.ABSTRACTDuring 2016 to 2020, GVI-1 type infectious bronchitis virus (IBV) strains were sporadically reported across China, indicating a new epidemic trend of the virus. Here we investigated the molecular characteristics and pathogenicity of two newly isolated GVI-1 type IBV virus strains (CK/CH/TJ1904 and CK/CH/NP2011) from infected chicken farms in China. Genetic evolution analysis of the S1 gene showed the highest homology with the GVI-1 representative strain, TC07-2. Phylogenetic analysis and recombination analysis of the vir...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Lu Sun Xinyan Tang Jingyi Qi Chunyan Zhang Jing Zhao Guozhong Zhang Ye Zhao Source Type: research

Cloning and sequencing of lsaE efflux pump gene from MDR Enterococci and its role in erythromycin resistance
Infect Genet Evol. 2021 Jul 19:105010. doi: 10.1016/j.meegid.2021.105010. Online ahead of print.ABSTRACTEnterococci are opportunistic members of intestinal microbiota with notable ability to transmit antimicrobial resistance genes. Among the different resistance mechanisms, multidrug efflux is evolving as a huge problem in conferring multidrug resistance to bacterial cells because these pumps extrude a broad range of antimicrobials. Therefore, the aim of this work was to evaluate role of efflux pumps in the development of multi-drug resistance in Enterococci through studying the antimicrobial resistance profiles of Enteroc...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Akram N Salah Nooran S Elleboudy Ghadir S El-Housseiny Mahmoud A Yassien Source Type: research

Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India
We report here a targeted risk-based study to investigate the presence of influenza A viruses at the migratory-wild-domestic bird interface across the major wetlands of central India's Maharashtra state during the winter migration season. The H9N2 viruses have been isolated and confirmed in 3.86% (33/854) of the fecal samples of resident birds. To investigate the genetic pools of H9N2 circulating in resident birds, we sequenced two isolates of H9N2 from distant wetlands. Sequence and phylogenetic analyses have shown that these viruses are triple reassortants, with HA, NA, NP, and M genes belonging to G1 sub-lineage (A/quai...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Richa Sood Naveen Kumar Suresh S Gokhe Atul Kumar Pateriya Sushant Bhat Sandeep Bhatia Sivasankar Panickan Anamika Mishra H V Murugkar Roma Dixit Deepali Shrivastava Pushpendra Singh Meghna Tripathi Vijendra Pal Singh Source Type: research

Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998-2005): Pre-rotavirus vaccine era
Infect Genet Evol. 2021 Jul 19:105007. doi: 10.1016/j.meegid.2021.105007. Online ahead of print.ABSTRACTHuman adenovirus (HAdV) is recognized as frequent cause of acute gastroenteritis and enteric viruses can be preserved in frozen stored feces for long periods of times. The purpose of the present study was to investigate enteric HAdV genotypic diversity in archival fecal specimens stored from 1998 to 2005 in order to understand the natural history of HAdV in diarrheal patients in Brazil before rotavirus vaccine introduction. A total of 3346 specimens were tested for HAdV using conventional PCR. Genotypes were identified b...
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Yasmin Fran ça Viana Pires de Souza Ellen Viana de Souza Lais Sampaio de Azevedo Roberta Salzone Medeiros Maria do Carmo Sampaio Tavares Timenetsky Adriana Luchs Source Type: research

SARS-CoV-2 as a betacoronavirus comprises five structural proteins?
Infect Genet Evol. 2021 Jul 19;94:105011. doi: 10.1016/j.meegid.2021.105011. Online ahead of print.NO ABSTRACTPMID:34293483 | PMC:PMC8289725 | DOI:10.1016/j.meegid.2021.105011 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 22, 2021 Category: Genetics & Stem Cells Authors: Milad Zandi Saber Soltani Kambiz Feyzi Source Type: research

Functional analysis of pde gene and its role in the pathogenesis of Xanthomonas oryzae pv. oryzicola
In this study, the function of EAL domain protein encoded by pde (FE36_09715) gene in the regulation of c-di-GMP was investigated. Results of this study, showed that the deletion of pde gene led to a significant reduction in the virulence of Xoc and was positively related to the reduction of extracellular polysaccharides production, biofilm formation, and flagellar motility. However, these significantly impaired properties from the ∆pde mutant strain were partially recovered in the complementary strain. In addition, the deletion of pde gene in Xoc strain YM15had no visible effect on the colony morphology, amylase, and pr...
Source: Infection, Genetics and Evolution - July 20, 2021 Category: Genetics & Stem Cells Authors: Xiaoyan Wan Jun Yang Waqar Ahmed Qi Liu Yanfang Wang Lanfang Wei Guanghai Ji Source Type: research

Evaluation of the usefulness of intermittent preventive treatment of malaria in pregnancy with sulfadoxine-pyrimethamine in a context with increased resistance of Plasmodium falciparum in Kingasani Hospital, Kinshasa in the Democratic Republic of Congo
Infect Genet Evol. 2021 Jul 17:105009. doi: 10.1016/j.meegid.2021.105009. Online ahead of print.ABSTRACTBACKGROUND: Increasing resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine (SP) threatens its usefulness for intermittent preventive treatment in pregnancy (IPTp-SP). The prophylactic effects of IPTp-SP on maternal malaria and adverse pregnancy outcomes were evaluated in Kingasani Hospital, Kinshasa in the Democratic Republic of Congo (DRC).METHODS: Laboring women (n = 844) and respective newborns were investigated. Blood samples collected from women were tested for malaria using rapid diagnostic test (RDT),...
Source: Infection, Genetics and Evolution - July 20, 2021 Category: Genetics & Stem Cells Authors: Nadine Kayiba Kalenda Doudou Malekita Yobi Vanessa Rodanis Tchakounang Kouoneyou Dieudonn é Makaba Mvumbi Pius Zakayi Kabututu Brecht Devleesschauwer Erick Mukomena Sompwe Patrick DeMol Marie-Pierre Hayette Georges Lelo Mvumbi Angel Rosas-Aguirre Paul Lu Source Type: research

Functional analysis of pde gene and its role in the pathogenesis of Xanthomonas oryzae pv. oryzicola
In this study, the function of EAL domain protein encoded by pde (FE36_09715) gene in the regulation of c-di-GMP was investigated. Results of this study, showed that the deletion of pde gene led to a significant reduction in the virulence of Xoc and was positively related to the reduction of extracellular polysaccharides production, biofilm formation, and flagellar motility. However, these significantly impaired properties from the ∆pde mutant strain were partially recovered in the complementary strain. In addition, the deletion of pde gene in Xoc strain YM15had no visible effect on the colony morphology, amylase, and pr...
Source: Infection, Genetics and Evolution - July 20, 2021 Category: Genetics & Stem Cells Authors: Xiaoyan Wan Jun Yang Waqar Ahmed Qi Liu Yanfang Wang Lanfang Wei Guanghai Ji Source Type: research

Evaluation of the usefulness of intermittent preventive treatment of malaria in pregnancy with sulfadoxine-pyrimethamine in a context with increased resistance of Plasmodium falciparum in Kingasani Hospital, Kinshasa in the Democratic Republic of Congo
Infect Genet Evol. 2021 Jul 17:105009. doi: 10.1016/j.meegid.2021.105009. Online ahead of print.ABSTRACTBACKGROUND: Increasing resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine (SP) threatens its usefulness for intermittent preventive treatment in pregnancy (IPTp-SP). The prophylactic effects of IPTp-SP on maternal malaria and adverse pregnancy outcomes were evaluated in Kingasani Hospital, Kinshasa in the Democratic Republic of Congo (DRC).METHODS: Laboring women (n = 844) and respective newborns were investigated. Blood samples collected from women were tested for malaria using rapid diagnostic test (RDT),...
Source: Infection, Genetics and Evolution - July 20, 2021 Category: Genetics & Stem Cells Authors: Nadine Kayiba Kalenda Doudou Malekita Yobi Vanessa Rodanis Tchakounang Kouoneyou Dieudonn é Makaba Mvumbi Pius Zakayi Kabututu Brecht Devleesschauwer Erick Mukomena Sompwe Patrick DeMol Marie-Pierre Hayette Georges Lelo Mvumbi Angel Rosas-Aguirre Paul Lu Source Type: research

Functional analysis of pde gene and its role in the pathogenesis of Xanthomonas oryzae pv. oryzicola
In this study, the function of EAL domain protein encoded by pde (FE36_09715) gene in the regulation of c-di-GMP was investigated. Results of this study, showed that the deletion of pde gene led to a significant reduction in the virulence of Xoc and was positively related to the reduction of extracellular polysaccharides production, biofilm formation, and flagellar motility. However, these significantly impaired properties from the ∆pde mutant strain were partially recovered in the complementary strain. In addition, the deletion of pde gene in Xoc strain YM15had no visible effect on the colony morphology, amylase, and pr...
Source: Infection, Genetics and Evolution - July 20, 2021 Category: Genetics & Stem Cells Authors: Xiaoyan Wan Jun Yang Waqar Ahmed Qi Liu Yanfang Wang Lanfang Wei Guanghai Ji Source Type: research

Evaluation of the usefulness of intermittent preventive treatment of malaria in pregnancy with sulfadoxine-pyrimethamine in a context with increased resistance of Plasmodium falciparum in Kingasani Hospital, Kinshasa in the Democratic Republic of Congo
Infect Genet Evol. 2021 Jul 17:105009. doi: 10.1016/j.meegid.2021.105009. Online ahead of print.ABSTRACTBACKGROUND: Increasing resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine (SP) threatens its usefulness for intermittent preventive treatment in pregnancy (IPTp-SP). The prophylactic effects of IPTp-SP on maternal malaria and adverse pregnancy outcomes were evaluated in Kingasani Hospital, Kinshasa in the Democratic Republic of Congo (DRC).METHODS: Laboring women (n = 844) and respective newborns were investigated. Blood samples collected from women were tested for malaria using rapid diagnostic test (RDT),...
Source: Infection, Genetics and Evolution - July 20, 2021 Category: Genetics & Stem Cells Authors: Nadine Kayiba Kalenda Doudou Malekita Yobi Vanessa Rodanis Tchakounang Kouoneyou Dieudonn é Makaba Mvumbi Pius Zakayi Kabututu Brecht Devleesschauwer Erick Mukomena Sompwe Patrick DeMol Marie-Pierre Hayette Georges Lelo Mvumbi Angel Rosas-Aguirre Paul Lu Source Type: research

Therapeutic target identification via differential genome analysis of antibiotic resistant Shigella sonneii and inhibitor evaluation against a selected drug target
In this study, we retrieved the antibiotic-resistant labeled whole genome sequences of the species from the PATRIC database and performed a pan-genome analysis to filter out core genes. Antibiotic resistance was studied in the core, accessory and unique genome. Core genes were utilized as seed substance for essentiality analysis and drug candidate assignment. Product of the gene aroG, i.e. chorismate biosynthetic process 3-deoxy-7-phosphoheptulonate synthase enzyme, responsible for aromatic amino acid family biosynthetic process, was taken for further downstream processing. Natural product libraries of flavonoids (n = 178)...
Source: Infection, Genetics and Evolution - July 19, 2021 Category: Genetics & Stem Cells Authors: Zarrin Basharat Muhammad Jahanzaib Noor Rahman Source Type: research

Therapeutic target identification via differential genome analysis of antibiotic resistant Shigella sonneii and inhibitor evaluation against a selected drug target
In this study, we retrieved the antibiotic-resistant labeled whole genome sequences of the species from the PATRIC database and performed a pan-genome analysis to filter out core genes. Antibiotic resistance was studied in the core, accessory and unique genome. Core genes were utilized as seed substance for essentiality analysis and drug candidate assignment. Product of the gene aroG, i.e. chorismate biosynthetic process 3-deoxy-7-phosphoheptulonate synthase enzyme, responsible for aromatic amino acid family biosynthetic process, was taken for further downstream processing. Natural product libraries of flavonoids (n = 178)...
Source: Infection, Genetics and Evolution - July 19, 2021 Category: Genetics & Stem Cells Authors: Zarrin Basharat Muhammad Jahanzaib Noor Rahman Source Type: research

Genetic and evolutionary analysis of SARS-CoV-2 circulating in the region surrounding Islamabad, Pakistan
This study highlights multiple SARS-CoV-2 lineages co-circulating during the peak of a second COVID-19 wave in Pakistan (Nov 2020-Feb 2021), with virus origins traced to the United States of America and Saudi Arabia. Ten SARS-CoV-2 positive samples were used for ONT library preparation. Sequence and phylogenetic analysis determined that the patients were infected with lineage B.1.1.250, originally identified in the United Kingdom and Bangladesh during March and April of 2020, and in circulation until the time of this study in Europe, USA and Australia. Lineage B.1.261 was originally identified in Saudi Arabia with widespre...
Source: Infection, Genetics and Evolution - July 16, 2021 Category: Genetics & Stem Cells Authors: Sana Tamim Nidia S Trovao Peter Thielen Tom Mehoke Brian Merritt Aamer Ikram Muhammad Salman Muhammad Masroor Alam Massab Umair Nazish Badar Adnan Khurshid Nayab Mehmood Source Type: research

Review: Why permanent control of cassava Brown streak disease in sub Saharan Africa have remained a dream come true since 1930s?
Infect Genet Evol. 2021 Jul 13:105001. doi: 10.1016/j.meegid.2021.105001. Online ahead of print.ABSTRACTEffective control of causal Ipomoviruses of Brown Streak Disease of Cassava (CBSD) in Africa have remained problematic besides remarkable 8 decades (1930s-2021) of research efforts. Reasons underlying resistance breakdown in genetically improved Cassava and expansion of host-and-vector range of Cassava Brown Streak Viruses are still unknown. Enormous genetic diversity of Cassava Brown Streak Viruses which is crucial for improving routine Reverse Transcription Polymerase Chain Reaction in CBSD-endemic regions of Africa is...
Source: Infection, Genetics and Evolution - July 16, 2021 Category: Genetics & Stem Cells Authors: Herieth Rhodes Mero Sylvester Leonard Lyantagaye Erik Bongcam Rudloff Source Type: research

Genetic and evolutionary analysis of SARS-CoV-2 circulating in the region surrounding Islamabad, Pakistan
This study highlights multiple SARS-CoV-2 lineages co-circulating during the peak of a second COVID-19 wave in Pakistan (Nov 2020-Feb 2021), with virus origins traced to the United States of America and Saudi Arabia. Ten SARS-CoV-2 positive samples were used for ONT library preparation. Sequence and phylogenetic analysis determined that the patients were infected with lineage B.1.1.250, originally identified in the United Kingdom and Bangladesh during March and April of 2020, and in circulation until the time of this study in Europe, USA and Australia. Lineage B.1.261 was originally identified in Saudi Arabia with widespre...
Source: Infection, Genetics and Evolution - July 16, 2021 Category: Genetics & Stem Cells Authors: Sana Tamim Nidia S Trovao Peter Thielen Tom Mehoke Brian Merritt Aamer Ikram Muhammad Salman Muhammad Masroor Alam Massab Umair Nazish Badar Adnan Khurshid Nayab Mehmood Source Type: research

Review: Why permanent control of cassava Brown streak disease in sub Saharan Africa have remained a dream come true since 1930s?
Infect Genet Evol. 2021 Jul 13:105001. doi: 10.1016/j.meegid.2021.105001. Online ahead of print.ABSTRACTEffective control of causal Ipomoviruses of Brown Streak Disease of Cassava (CBSD) in Africa have remained problematic besides remarkable 8 decades (1930s-2021) of research efforts. Reasons underlying resistance breakdown in genetically improved Cassava and expansion of host-and-vector range of Cassava Brown Streak Viruses are still unknown. Enormous genetic diversity of Cassava Brown Streak Viruses which is crucial for improving routine Reverse Transcription Polymerase Chain Reaction in CBSD-endemic regions of Africa is...
Source: Infection, Genetics and Evolution - July 16, 2021 Category: Genetics & Stem Cells Authors: Herieth Rhodes Mero Sylvester Leonard Lyantagaye Erik Bongcam Rudloff Source Type: research

Genetic and evolutionary analysis of SARS-CoV-2 circulating in the region surrounding Islamabad, Pakistan
This study highlights multiple SARS-CoV-2 lineages co-circulating during the peak of a second COVID-19 wave in Pakistan (Nov 2020-Feb 2021), with virus origins traced to the United States of America and Saudi Arabia. Ten SARS-CoV-2 positive samples were used for ONT library preparation. Sequence and phylogenetic analysis determined that the patients were infected with lineage B.1.1.250, originally identified in the United Kingdom and Bangladesh during March and April of 2020, and in circulation until the time of this study in Europe, USA and Australia. Lineage B.1.261 was originally identified in Saudi Arabia with widespre...
Source: Infection, Genetics and Evolution - July 16, 2021 Category: Genetics & Stem Cells Authors: Sana Tamim Nidia S Trovao Peter Thielen Tom Mehoke Brian Merritt Aamer Ikram Muhammad Salman Muhammad Masroor Alam Massab Umair Nazish Badar Adnan Khurshid Nayab Mehmood Source Type: research

Review: Why permanent control of cassava Brown streak disease in sub Saharan Africa have remained a dream come true since 1930s?
Infect Genet Evol. 2021 Jul 13:105001. doi: 10.1016/j.meegid.2021.105001. Online ahead of print.ABSTRACTEffective control of causal Ipomoviruses of Brown Streak Disease of Cassava (CBSD) in Africa have remained problematic besides remarkable 8 decades (1930s-2021) of research efforts. Reasons underlying resistance breakdown in genetically improved Cassava and expansion of host-and-vector range of Cassava Brown Streak Viruses are still unknown. Enormous genetic diversity of Cassava Brown Streak Viruses which is crucial for improving routine Reverse Transcription Polymerase Chain Reaction in CBSD-endemic regions of Africa is...
Source: Infection, Genetics and Evolution - July 16, 2021 Category: Genetics & Stem Cells Authors: Herieth Rhodes Mero Sylvester Leonard Lyantagaye Erik Bongcam Rudloff Source Type: research

Comparative population genetic structure of two ixodid tick species (Acari:Ixodidae) (Ixodes ovatus and Haemaphysalis flava) in Niigata prefecture, Japan
In this study, we characterized the population genetic structure and inferred genetic divergence in two widespread and abundant ixodid species, Ixodes ovatus and Haemaphysalis flava. Our hypothesis was that genetic divergence would be high in I. ovatus because of the low mobility of their small rodent hosts of immature I. ovatus would limit their gene flow compared to more mobile avian hosts of immature H. flava. We collected 320 adult I. ovatus from 29 locations and 223 adult H. flava from 17 locations across Niigata Prefecture, Japan, and investigated their genetic structure using DNA sequences from fragments of two mito...
Source: Infection, Genetics and Evolution - July 13, 2021 Category: Genetics & Stem Cells Authors: Maria Angenica F Regilme Megumi Sato Tsutomu Tamura Reiko Arai Marcello Otake Sato Sumire Ikeda Maribet Gamboa Michael T Monaghan Kozo Watanabe Source Type: research

Comparative population genetic structure of two ixodid tick species (Acari:Ixodidae) (Ixodes ovatus and Haemaphysalis flava) in Niigata prefecture, Japan
In this study, we characterized the population genetic structure and inferred genetic divergence in two widespread and abundant ixodid species, Ixodes ovatus and Haemaphysalis flava. Our hypothesis was that genetic divergence would be high in I. ovatus because of the low mobility of their small rodent hosts of immature I. ovatus would limit their gene flow compared to more mobile avian hosts of immature H. flava. We collected 320 adult I. ovatus from 29 locations and 223 adult H. flava from 17 locations across Niigata Prefecture, Japan, and investigated their genetic structure using DNA sequences from fragments of two mito...
Source: Infection, Genetics and Evolution - July 13, 2021 Category: Genetics & Stem Cells Authors: Maria Angenica F Regilme Megumi Sato Tsutomu Tamura Reiko Arai Marcello Otake Sato Sumire Ikeda Maribet Gamboa Michael T Monaghan Kozo Watanabe Source Type: research

Comparative population genetic structure of two ixodid tick species (Acari:Ixodidae) (Ixodes ovatus and Haemaphysalis flava) in Niigata prefecture, Japan
In this study, we characterized the population genetic structure and inferred genetic divergence in two widespread and abundant ixodid species, Ixodes ovatus and Haemaphysalis flava. Our hypothesis was that genetic divergence would be high in I. ovatus because of the low mobility of their small rodent hosts of immature I. ovatus would limit their gene flow compared to more mobile avian hosts of immature H. flava. We collected 320 adult I. ovatus from 29 locations and 223 adult H. flava from 17 locations across Niigata Prefecture, Japan, and investigated their genetic structure using DNA sequences from fragments of two mito...
Source: Infection, Genetics and Evolution - July 13, 2021 Category: Genetics & Stem Cells Authors: Maria Angenica F Regilme Megumi Sato Tsutomu Tamura Reiko Arai Marcello Otake Sato Sumire Ikeda Maribet Gamboa Michael T Monaghan Kozo Watanabe Source Type: research

Genotyping of Ascaris spp. infecting humans and pigs in Italy, Slovakia and Colombia
CONCLUSIONS: This study updates and extends the current understanding of Ascaris species and genotypes circulating in different epidemiological scenarios, with particular attention to the inclusion of human-derived Ascaris in the phylogenetic cluster C. Despite the evidence of HW equilibrium in the ITS in pig-derived Italian samples, the amount of genetic variation seems to support the existence of two closely related species.PMID:34252615 | DOI:10.1016/j.meegid.2021.104997 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 12, 2021 Category: Genetics & Stem Cells Authors: Serena Cavallero Silvia Rond ón Ivan Acevedo Monterrosa Viliam Šnábel Ingrid Papajov á M ária Goldová Gabriela Štrkolcová Luis Caraballo Nathalie Acevedo Stefano D'Amelio Source Type: research

New infections by SARS-CoV-2 variants of concern after natural infections and post-vaccination in Rio de Janeiro, Brazil
We present here several examples of how natural infections or vaccination may not be fully capable of conferring sterilizing immunity against VOC.PMID:34252616 | DOI:10.1016/j.meegid.2021.104998 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - July 12, 2021 Category: Genetics & Stem Cells Authors: Livia R Goes Juliana D Siqueira Marianne M Garrido Brunna M Alves Ana Cristina P M Pereira Claudia Cicala James Arthos Jo ão P B Viola Marcelo A Soares INCA COVID-19 Task Force ˆ Source Type: research

Erythrocyte miRNA regulators and malarial pathophysiology
Infect Genet Evol. 2021 Jul 9:105000. doi: 10.1016/j.meegid.2021.105000. Online ahead of print.ABSTRACTPathophysiology of Plasmodium falciparum and Plasmodium vivax in malaria vis a vis host and the parasite genome interactions has been deciphered recently to present the biology of cerebral malaria, severe anaemia and placental malaria. Small non-coding RNAs have exhibited their potential to be considered as indicators and regulators of diseases. The malarial pathologies and their associated mechanisms mediated by miRNAs and their role in haematopoiesis and red cell-related disorders are elucidated. Evidence of miRNA carry...
Source: Infection, Genetics and Evolution - July 12, 2021 Category: Genetics & Stem Cells Authors: Sowmya R Prabhu Akshay P Ware Abdul Vahab Saadi Source Type: research

New insights into Human Pegivirus-1 (HPgV-1) genotypes diversity in sub-Saharan Africa
Infect Genet Evol. 2021 Jul 8:104995. doi: 10.1016/j.meegid.2021.104995. Online ahead of print.ABSTRACTIn the framework of a viral discovery research program using metagenomics, Human Pegivirus-1 reads (HPgV-1, formerly known as GBV-C) were detected in plasma pools of healthy blood donors from seven sub-Saharan African countries. For five of these countries, Mauritania, Mali, Niger, Burundi and Madagascar, no data about HPgV-1 genotypes was reported to date. To confirm our metagenomic findings and further investigate the genotype diversity and distribution of HPgV-1 in Africa, 400 blood donations from these five localities...
Source: Infection, Genetics and Evolution - July 11, 2021 Category: Genetics & Stem Cells Authors: Virginie Sauvage Johanna Gomez Laure Boizeau Mathias Vandenbogaert L éa Barbier Claude Tayou Tagny Aim ée Olivat Rakoto Alson Pascal Bizimana Sekou Oumar Coulibaly Mohamed Abdallahi Boullahi Hadiza Soumana Guy Mbensa Val érie Caro Syria Laperche Source Type: research