Phenotypic and molecular characterization of multi-drug resistant Klebsiella spp. isolates recovered from clinical settings
In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used for in-depth molecular characterization. in silico typing was used to determine the resistance genes, virulence factors, Inc. groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. blaNDM, blaOXA-48, and ...
Source: Infection, Genetics and Evolution - March 14, 2024 Category: Genetics & Stem Cells Authors: Hassan Diab Kelven Rahy Tamima Jisr Mira El Chaar Edmond Abboud Sima Tokajian Source Type: research
Phenotypic and molecular characterization of multi-drug resistant Klebsiella spp. isolates recovered from clinical settings
In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used for in-depth molecular characterization. in silico typing was used to determine the resistance genes, virulence factors, Inc. groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. blaNDM, blaOXA-48, and ...
Source: Infection, Genetics and Evolution - March 14, 2024 Category: Genetics & Stem Cells Authors: Hassan Diab Kelven Rahy Tamima Jisr Mira El Chaar Edmond Abboud Sima Tokajian Source Type: research
Phenotypic and molecular characterization of multi-drug resistant Klebsiella spp. isolates recovered from clinical settings
In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used for in-depth molecular characterization. in silico typing was used to determine the resistance genes, virulence factors, Inc. groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. blaNDM, blaOXA-48, and ...
Source: Infection, Genetics and Evolution - March 14, 2024 Category: Genetics & Stem Cells Authors: Hassan Diab Kelven Rahy Tamima Jisr Mira El Chaar Edmond Abboud Sima Tokajian Source Type: research
Phenotypic and molecular characterization of multi-drug resistant Klebsiella spp. isolates recovered from clinical settings
In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used for in-depth molecular characterization. in silico typing was used to determine the resistance genes, virulence factors, Inc. groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. blaNDM, blaOXA-48, and ...
Source: Infection, Genetics and Evolution - March 14, 2024 Category: Genetics & Stem Cells Authors: Hassan Diab Kelven Rahy Tamima Jisr Mira El Chaar Edmond Abboud Sima Tokajian Source Type: research
A novel metagenomic approach uncovers phage genes as markers for increased disinfectant tolerance in mixed Listeria monocytogenes communities
In this study, we applied a novel metagenomic approach to detect genes associated with disinfectant tolerance in mixed L. monocytogenes planktonic communities. Two communities, consisting of 71 and 80 isolates each, were treated with the food industry disinfectants benzalkonium chloride (BC, 1.75 mg/L) or peracetic acid (PAA, 38 mg/L). The communities were subjected to metagenomic sequencing and differences in individual gene abundances between biocide-free control communities and biocide-treated communities were determined. A significant increase in the abundance of Listeria phage-associated genes was observed in both com...
Source: Infection, Genetics and Evolution - March 11, 2024 Category: Genetics & Stem Cells Authors: Agnete Kirstine Karlsmose Mirena Ivanova Martin Laage Kragh Jette Sejer Kjeldgaard Saria Otani Christina Aaby Svendsen Bojan Papi ć Irena Zdovc Taurai Tasara Roger Stephan Even Heir Solveig Langsrud Trond M øretrø Paw Dalgaard Annette Fagerlund Lisbeth Source Type: research
A novel metagenomic approach uncovers phage genes as markers for increased disinfectant tolerance in mixed Listeria monocytogenes communities
In this study, we applied a novel metagenomic approach to detect genes associated with disinfectant tolerance in mixed L. monocytogenes planktonic communities. Two communities, consisting of 71 and 80 isolates each, were treated with the food industry disinfectants benzalkonium chloride (BC, 1.75 mg/L) or peracetic acid (PAA, 38 mg/L). The communities were subjected to metagenomic sequencing and differences in individual gene abundances between biocide-free control communities and biocide-treated communities were determined. A significant increase in the abundance of Listeria phage-associated genes was observed in both com...
Source: Infection, Genetics and Evolution - March 11, 2024 Category: Genetics & Stem Cells Authors: Agnete Kirstine Karlsmose Mirena Ivanova Martin Laage Kragh Jette Sejer Kjeldgaard Saria Otani Christina Aaby Svendsen Bojan Papi ć Irena Zdovc Taurai Tasara Roger Stephan Even Heir Solveig Langsrud Trond M øretrø Paw Dalgaard Annette Fagerlund Lisbeth Source Type: research
A novel metagenomic approach uncovers phage genes as markers for increased disinfectant tolerance in mixed Listeria monocytogenes communities
In this study, we applied a novel metagenomic approach to detect genes associated with disinfectant tolerance in mixed L. monocytogenes planktonic communities. Two communities, consisting of 71 and 80 isolates each, were treated with the food industry disinfectants benzalkonium chloride (BC, 1.75 mg/L) or peracetic acid (PAA, 38 mg/L). The communities were subjected to metagenomic sequencing and differences in individual gene abundances between biocide-free control communities and biocide-treated communities were determined. A significant increase in the abundance of Listeria phage-associated genes was observed in both com...
Source: Infection, Genetics and Evolution - March 11, 2024 Category: Genetics & Stem Cells Authors: Agnete Kirstine Karlsmose Mirena Ivanova Martin Laage Kragh Jette Sejer Kjeldgaard Saria Otani Christina Aaby Svendsen Bojan Papi ć Irena Zdovc Taurai Tasara Roger Stephan Even Heir Solveig Langsrud Trond M øretrø Paw Dalgaard Annette Fagerlund Lisbeth Source Type: research
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infecte...
Source: Infection, Genetics and Evolution - March 3, 2024 Category: Genetics & Stem Cells Authors: Baoping Guo None Cairen Jianyong Wu Wenli Wang Bingjie Wang Yuan Ren Mayire Aizezi Rongsheng Mi Li Zhao Hao Wen Source Type: research
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infecte...
Source: Infection, Genetics and Evolution - March 3, 2024 Category: Genetics & Stem Cells Authors: Baoping Guo None Cairen Jianyong Wu Wenli Wang Bingjie Wang Yuan Ren Mayire Aizezi Rongsheng Mi Li Zhao Hao Wen Source Type: research
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infecte...
Source: Infection, Genetics and Evolution - March 3, 2024 Category: Genetics & Stem Cells Authors: Baoping Guo None Cairen Jianyong Wu Wenli Wang Bingjie Wang Yuan Ren Mayire Aizezi Rongsheng Mi Li Zhao Hao Wen Source Type: research
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infecte...
Source: Infection, Genetics and Evolution - March 3, 2024 Category: Genetics & Stem Cells Authors: Baoping Guo None Cairen Jianyong Wu Wenli Wang Bingjie Wang Yuan Ren Mayire Aizezi Rongsheng Mi Li Zhao Hao Wen Source Type: research
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infecte...
Source: Infection, Genetics and Evolution - March 3, 2024 Category: Genetics & Stem Cells Authors: Baoping Guo None Cairen Jianyong Wu Wenli Wang Bingjie Wang Yuan Ren Mayire Aizezi Rongsheng Mi Li Zhao Hao Wen Source Type: research
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infecte...
Source: Infection, Genetics and Evolution - March 3, 2024 Category: Genetics & Stem Cells Authors: Baoping Guo None Cairen Jianyong Wu Wenli Wang Bingjie Wang Yuan Ren Mayire Aizezi Rongsheng Mi Li Zhao Hao Wen Source Type: research
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infecte...
Source: Infection, Genetics and Evolution - March 3, 2024 Category: Genetics & Stem Cells Authors: Baoping Guo None Cairen Jianyong Wu Wenli Wang Bingjie Wang Yuan Ren Mayire Aizezi Rongsheng Mi Li Zhao Hao Wen Source Type: research
Revisiting genetic diversity in Echinococcus multilocularis, the role for EmsB microsatellite: A commentary
Infect Genet Evol. 2024 Feb 29:105580. doi: 10.1016/j.meegid.2024.105580. Online ahead of print.NO ABSTRACTPMID:38431092 | DOI:10.1016/j.meegid.2024.105580 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - March 2, 2024 Category: Genetics & Stem Cells Authors: Mohammad Ali Mohammadi Maid Fasihi Harandi Source Type: research
