Detection of multiple recombinations of avian coronavirus in South Korea by whole-genome analysis
In this study, complete genome sequences of 23 IBV isolates in Korea from 2010 to 2020 were obtained using next-generation sequencing, and their phylogenetic relationship and recombination events were analyzed. Phylogenetic analysis based on the S1 gene showed that all isolates belonged to the GI-19 lineage and were divided into five subgroups (KM91-like, K40/09-like, and QX-like II to IV). Among the 23 isolates, 14 recombinants were found, including frequent recombination between KM91-like and QX-like strains. In addition, it was observed that other lineages, such as GI-1, GI-13, and GI-16, were involved in recombination....
Source: Infection, Genetics and Evolution - February 3, 2024 Category: Genetics & Stem Cells Authors: Da-Won Kim Ji-Yun Kim Dong-Wook Lee Hyuk-Chae Lee Chang-Seon Song Dong-Hun Lee Jung-Hoon Kwon Source Type: research
Molecular detection of Coxiella burnetii in ticks collected from Iran
In conclusion, this study emphasizes the role of ticks as potential carriers of C. burneti. The results indicate the importance of cattle, sheep, goats, camels and dogs in Hormozgan region as effective factors in the epidemiology of Q fever and its impact on public health. In addition, a high degree of similarity (from 99% to 100%) was observed between IS1111 and icd genes in this study and recorded sequences from different regions of the world.PMID:38307395 | DOI:10.1016/j.meegid.2024.105562 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - February 2, 2024 Category: Genetics & Stem Cells Authors: Ahmad Enferadi Saeedeh Sarani Shirin Mohammadipour Sayyed Jafar Hasani Afshin Ajdari Maryam Najafi Asl Peyman Khademi Source Type: research
Host genetics and the profile of COVID-19 in indigenous people from the Brazilian Amazon: A pilot study with variants of the ACE1, ACE2 and TMPRSS2 genes
Infect Genet Evol. 2024 Feb 1;118:105564. doi: 10.1016/j.meegid.2024.105564. Online ahead of print.ABSTRACTThis pilot study aimed to investigate genetic factors that may have contributed to the milder clinical outcomes of COVID-19 in Brazilian indigenous populations. 263 Indigenous from the Araweté, Kararaô, Parakanã, Xikrin do Bacajá, Kayapó and Munduruku peoples were analyzed, 55.2% women, ages ranging from 10 to 95 years (average 49.5 ± 20.7). Variants in genes involved in the entry of SARS-CoV-2 into the host cell (ACE1 rs1799752 I/D, ACE2 rs2285666 C/T, ACE2 rs73635825 A/G and TMPRSS2 rs123297605 C/T), were geno...
Source: Infection, Genetics and Evolution - February 2, 2024 Category: Genetics & Stem Cells Authors: Eliene Rodrigues Putira Sacuena Carlos Neandro Cordeiro Lima Isabella Nogueira Abreu Lucas Matheus Cavalcante da Silva Lilian Karen Goes Belleza Renan Barbosa Lemes Gilderl ânio Santana de Araújo Hilton Pereira da Silva Ant ônio Carlos Rosário Vallino Source Type: research
Trypanosomatid diversity in a bat community of an urban area in Campo Grande, Mato Grosso do Sul, Brazil
We report an overall trypanosomatid detection rate of 34.2% (n = 81), involving 11 out of 13 sampled bat species. We identified six species of trypanosomatids from 61 bats by analyzing SSU rRNA and/or kDNA: Trypanosoma cruzi DTU TcI, T. c. marinkellei, T. dionisii, Leishmania infantum, L. amazonensis, and T. janseni, with this latter being detected by hemoculture for the first time in a bat species. We also detected a Molecular Operational Taxonomic Unit, Trypanosoma sp. DID, in the phyllostomids Glossophaga soricina and Platyrrhinus lineatus. The highest trypanosomatid richness was observed for Sturnira lilium, which host...
Source: Infection, Genetics and Evolution - February 1, 2024 Category: Genetics & Stem Cells Authors: Jaire Marinho Torres Carina Elisei de Oliveira Filipe Martins Santos Nayara Yoshie Sano Érica Verneque Martinez Fernanda Moreira Alves Luiz Eduardo Roland Tavares Andr é Luiz Rodrigues Roque Ana Maria Jansen Heitor Miraglia Herrera Source Type: research
Genome analysis deciphered Chryseobacterium indicum is a distinct species associated with freshwater pufferfish
Infect Genet Evol. 2024 Jan 26;118:105561. doi: 10.1016/j.meegid.2024.105561. Online ahead of print.ABSTRACTA bacterium, strain PS-8T of the genus Chryseobacterium, was isolated from the skin of freshwater pufferfish (Tetraodon cutcutia). Strain PS-8T is a Gram-negative, aerobic, non-motile, and rod-shaped bacterium. Colonies appear in yellowish-orange colors. The major cellular fatty acids were C15:0 iso, C17:0 iso 3OH, C15:0 iso 3OH, and C11:0 anteiso. The predominant polar lipids were phosphatidylethanolamine and amino lipids. The genome size is 4.83 Mb. The G + C content was 35.6%. The in silico dDDH homology, ANI, and...
Source: Infection, Genetics and Evolution - January 27, 2024 Category: Genetics & Stem Cells Authors: Manish Prakash Victor Ritu Rani Archana Kujur Lipika Das Subrata K Das Source Type: research
Consanguineous marriages increase the incidence of recurrent tuberculosis: Evidence from whole exome sequencing
CONCLUSION: Our findings indicate the significant contribution of the IL-12/IFN-γ is in combating infections due to mycobacterium. Among Pakistani patients born to consanguineous marriages, the identified mutations in the IL-12Rβ-1 gene provide insights into the genetic basis of severe BCG infections and recurrent tuberculosis. The study highlights the potential utility of newborn screening in regions with mandatory BCG vaccination, enabling early detection and intervention for primary immunodeficiencies associated with mycobacterial infections. Moreover, the study suggests at the potential role of other related genes su...
Source: Infection, Genetics and Evolution - January 24, 2024 Category: Genetics & Stem Cells Authors: Noor Ul Akbar Sajjad Ahmad Taj Ali Khan Muhammad Tayyeb Naheed Akhter Laraib Shafiq Shahid Niaz Khan Mohammad Mahtab Alam Alduwish Manal Abdullah Muhammad Fayyaz Ur Rehman Majed A Bajaber Muhammad Safwan Akram Source Type: research
Serological and molecular epidemiological investigation of Mediterranean spotted fever in Yunnan Province, China
CONCLUSIONS: This research reveals a correlation between MSF prevalence, geography, and climate in Yunnan. The paucity of prior studies underscores MSF's potential diagnostic challenges in the region. Comprehensive understanding of the pathogen's distribution is pivotal for intervention. Given the study's scope and Yunnan's unique setting, additional research is advocated.PMID:38262571 | DOI:10.1016/j.meegid.2024.105560 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - January 23, 2024 Category: Genetics & Stem Cells Authors: Suyi Luo Shuyue Yang Aihua Liu Hanxin Wu Li Gao Xinya Wu Yan Dong Bingxue Li Weijiang Ma Li Peng Fukai Bao Source Type: research
Phylogenetic congruence of Plasmodium spp. and wild ungulate hosts in the Peruvian Amazon
We report for the first time, the presence of parasite lineages closely related to Plasmodium odocoilei clade 1 and clade 2 in free-ranging South American red-brocket deer (Mazama americana; 44.4%, 4/9) and gray-brocket deer (Mazama nemorivaga; 50.0%, 1/2). We performed PCR-based analysis of blood samples from 47 ungulates of five different species collected during subsistence hunting by an indigenous community in the Peruvian Amazon. We detected Plasmodium malariae/brasilianum lineage in a sample from red-brocket deer. However, no parasite DNA was detected in collared peccary (Pecari tajacu; 0.0%, 0/10), white-lipped pecc...
Source: Infection, Genetics and Evolution - January 21, 2024 Category: Genetics & Stem Cells Authors: Gabriela M Ulloa Alex D Greenwood Omar E Cornejo Frederico Ozanan Barros Monteiro Alessandra Scofield Meddly L Santolalla Robles Andres G Lescano Pedro Mayor Source Type: research
Molecular epidemiology and phylogeny of the emerging zoonotic virus Rocahepevirus: A global genetic analysis
This study conducted a global genetic analysis of Rocahepevirus, aiming to furnish comprehensive molecular insights and promote further research. We retrieved 817 Rocahepevirus sequences from the GenBank database through October 31, 2023, categorizing them according to research, sample collection area and date, genotype, host, and sequence length. Subsequently, we conducted descriptive epidemiological, phylogenetic evolutionary, and protein polymorphism (in length and identity) analyses on these sequences. Rocahepevirus genomes were identified across twenty-eight countries, predominantly in Asia (71.73%, 586/817) and Europ...
Source: Infection, Genetics and Evolution - January 20, 2024 Category: Genetics & Stem Cells Authors: Han Wu Lu Zhou Fengge Wang Zixiang Chen Yihan Lu Source Type: research
Genetic polymorphisms of Plasmodium vivax ookinete (sexual stage) surface proteins (Pvs25 and Pvs28) from Thailand
The objective of this study was to investigate the genetic diversity of Pvs25 and Pvs28 in endemic areas of Thailand. P. vivax clinical isolates collected from Thai-neighboring border areas were analyzed using polymerase chain reaction and sequencing method. Three and 14 amino acid substitutions were observed in 43 Pvs25 and 48 Pvs28 sequences, respectively. Three haplotypes in Pvs25 and 14 haplotypes with 5-7 GSGGE/D tandem repeats in Pvs28 were identified. The nucleotide diversity of pvs25 (π = 0.00059) had lower level than pvs28 (π = 0.00517). Tajima's D value for both pvs25 and pvs28 genes were negative while no sign...
Source: Infection, Genetics and Evolution - January 20, 2024 Category: Genetics & Stem Cells Authors: Jiraporn Kuesap Nutnicha Suphakhonchuwong Kanchana Rungsihirunrat Source Type: research
Genetic characteristics of a novel HIV-1 circulating recombinant form (CRF128_07B) identified among MSM in Guangdong Province, China
CONCLUSIONS: These findings described a novel HIV-1 CRF identified from MSM in Guangdong Province. This is the first detection of a CRF comprising CRF07_BC and subtype B. The present finding highlights the urgent need for continuous molecular screening and the epidemic surveillance within the MSM populations.PMID:38242185 | DOI:10.1016/j.meegid.2024.105555 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - January 19, 2024 Category: Genetics & Stem Cells Authors: Yun Lan Ruolei Xin Xizi Deng Feng Li Xuemei Ling Kun Zeng Chuyu Zhang Linghua Li Fengyu Hu Source Type: research
Understanding SARS-CoV-2 spike glycoprotein clusters and their impact on immunity of the population from Rio Grande do Norte, Brazil
Infect Genet Evol. 2024 Jan 17;118:105556. doi: 10.1016/j.meegid.2024.105556. Online ahead of print.ABSTRACTSARS-CoV-2 genome underwent mutations since it started circulating within the human population. The aim of this study was to understand the fluctuation of the spike clusters concomitant to the population immunity either due to natural infection and/or vaccination in a state of Brazil that had both high rate of natural infection and vaccination coverage. A total of 1725 SARS-CoV-2 sequences from the state of Rio Grande do Norte, Brazil, were retrieved from GISAID and subjected to cluster analysis. Immunoinformatics we...
Source: Infection, Genetics and Evolution - January 19, 2024 Category: Genetics & Stem Cells Authors: Diego Gomes Teixeira Jo ão Firmino Rodrigues-Neto Dayse Caroline Severiano da Cunha Selma Maria Bezerra Jeronimo Source Type: research
Gene polymorphisms of IL-17A and bacterial meningitis in Angolan children
This study suggests an association of IL-17A genetic variations with susceptibility and outcome of bacterial meningitis in Angolan children.PMID:38228216 | DOI:10.1016/j.meegid.2024.105553 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - January 16, 2024 Category: Genetics & Stem Cells Authors: Johanna Ter äsjärvi Elina Tenhu Manuel Leite Cruzeiro Okko Savonius Emilie Rugemalira Qiushui He Tuula Pelkonen Source Type: research
In vivo biological validation of in silico analysis: A novel approach for predicting the effects of TLR4 exon 3 polymorphisms on brucellosis
In conclusion, this study combines multidisciplinary approaches to evaluate the biological and structural effects of SNPs on protein function.PMID:38218390 | DOI:10.1016/j.meegid.2024.105552 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - January 13, 2024 Category: Genetics & Stem Cells Authors: Antonio Gentile Andrea Fulgione Barbara Auzino Valentina Iovane Daniela Gallo Raffaele Garramone Nunzia Iaccarino Antonio Randazzo Giuseppe Iovane Paola Cuomo Rosanna Capparelli Domenico Iannelli Source Type: research
Evolutionary analysis of major histocompatibility complex variants in chytrid-resistant and susceptible amphibians
In this study, I conducted recombination and selection tests on functional MHC IIß1 alleles from an Asian Bd-resistant anuran species (Bufo gargarizans) and an Australasian Bd-susceptible species (Litoria caerulea). Recombination at the same site was identified in both species, supporting the hypothesis that recombination contributes to MHC IIß1 diversity in amphibians. Positive selection was observed in MHC IIß1 alleles in both species. In L. caerulea, at least four amino acid sites were identified under significant positive selection in the MHC IIß1, whereas these sites were either negatively selected or conserved in...
Source: Infection, Genetics and Evolution - January 12, 2024 Category: Genetics & Stem Cells Authors: Minjie Fu Source Type: research
