Characterization of uropathogenic Escherichia coli phylogroups associated with antimicrobial resistance, virulence factor distribution, and virulence-related phenotypes
In this study, we compared the characteristics of different uropathogenic Escherichia coli phylogroups. A total of 844 E. coli isolated from urine were enrolled and the antimicrobial susceptibility of E. coli to 22 antibiotics was determined by disk diffusion test. The distribution of phylogroups and 20 virulence factor genes was determined by PCR. Phenotypes associated with bacterial virulence, including motility, biofilm formation, and the production of curli and siderophore, were examined. Phylogroup B2 was dominant in our isolates (64.8%), followed by phylogroups D (8.6%), B1 (7.8%), F (6.0%), C (4.5%), A (3.1%), untyp...
Source: Infection, Genetics and Evolution - August 27, 2023 Category: Genetics & Stem Cells Authors: Ming-Cheng Wang Yu-Hua Fan Yen-Zhen Zhang Carl Jay Ballena Bregente Wei-Hung Lin Chao-An Chen Tzu-Ping Lin Cheng-Yen Kao Source Type: research
Genotypic diversity and recombination of norovirus GI.6[P11] associated acute gastroenteritis outbreaks in Beijing, China, from 2016 to 2019
This study aimed to learn the epidemiological characteristics of 39 GI.6[P11] NoV outbreaks in Beijing, China, from 2016 to 2019 and to analyze the genetic diversity and phylogenetic process of GI.6[P11] strains. The Bayesian phylogenetic analysis of partial VP1 and RNA-dependent RNA polymerase (RdRp) genes showed that GI.6[P11] strains were clustered into four subclades. Eleven whole genome sequences were obtained through the amplicon sequencing with 16 pairs of newly designed primers. The phylogenetic trees based on the whole genome and ORF1, 2, and 3 showed that the clustering of the 11 strains was consistent with that ...
Source: Infection, Genetics and Evolution - August 19, 2023 Category: Genetics & Stem Cells Authors: Jiamei Fu Lingyu Shen Weihong Li Hanqiu Yan Baiwei Liu Yu Wang Yi Tian Lei Jia Quanyi Wang Daitao Zhang Zhiyong Gao Source Type: research
Genotypic diversity and recombination of norovirus GI.6[P11] associated acute gastroenteritis outbreaks in Beijing, China, from 2016 to 2019
This study aimed to learn the epidemiological characteristics of 39 GI.6[P11] NoV outbreaks in Beijing, China, from 2016 to 2019 and to analyze the genetic diversity and phylogenetic process of GI.6[P11] strains. The Bayesian phylogenetic analysis of partial VP1 and RNA-dependent RNA polymerase (RdRp) genes showed that GI.6[P11] strains were clustered into four subclades. Eleven whole genome sequences were obtained through the amplicon sequencing with 16 pairs of newly designed primers. The phylogenetic trees based on the whole genome and ORF1, 2, and 3 showed that the clustering of the 11 strains was consistent with that ...
Source: Infection, Genetics and Evolution - August 19, 2023 Category: Genetics & Stem Cells Authors: Jiamei Fu Lingyu Shen Weihong Li Hanqiu Yan Baiwei Liu Yu Wang Yi Tian Lei Jia Quanyi Wang Daitao Zhang Zhiyong Gao Source Type: research
Genotypic diversity and recombination of norovirus GI.6[P11] associated acute gastroenteritis outbreaks in Beijing, China, from 2016 to 2019
This study aimed to learn the epidemiological characteristics of 39 GI.6[P11] NoV outbreaks in Beijing, China, from 2016 to 2019 and to analyze the genetic diversity and phylogenetic process of GI.6[P11] strains. The Bayesian phylogenetic analysis of partial VP1 and RNA-dependent RNA polymerase (RdRp) genes showed that GI.6[P11] strains were clustered into four subclades. Eleven whole genome sequences were obtained through the amplicon sequencing with 16 pairs of newly designed primers. The phylogenetic trees based on the whole genome and ORF1, 2, and 3 showed that the clustering of the 11 strains was consistent with that ...
Source: Infection, Genetics and Evolution - August 19, 2023 Category: Genetics & Stem Cells Authors: Jiamei Fu Lingyu Shen Weihong Li Hanqiu Yan Baiwei Liu Yu Wang Yi Tian Lei Jia Quanyi Wang Daitao Zhang Zhiyong Gao Source Type: research
Genotypic diversity and recombination of norovirus GI.6[P11] associated acute gastroenteritis outbreaks in Beijing, China, from 2016 to 2019
This study aimed to learn the epidemiological characteristics of 39 GI.6[P11] NoV outbreaks in Beijing, China, from 2016 to 2019 and to analyze the genetic diversity and phylogenetic process of GI.6[P11] strains. The Bayesian phylogenetic analysis of partial VP1 and RNA-dependent RNA polymerase (RdRp) genes showed that GI.6[P11] strains were clustered into four subclades. Eleven whole genome sequences were obtained through the amplicon sequencing with 16 pairs of newly designed primers. The phylogenetic trees based on the whole genome and ORF1, 2, and 3 showed that the clustering of the 11 strains was consistent with that ...
Source: Infection, Genetics and Evolution - August 19, 2023 Category: Genetics & Stem Cells Authors: Jiamei Fu Lingyu Shen Weihong Li Hanqiu Yan Baiwei Liu Yu Wang Yi Tian Lei Jia Quanyi Wang Daitao Zhang Zhiyong Gao Source Type: research
Genotypic diversity and recombination of norovirus GI.6[P11] associated acute gastroenteritis outbreaks in Beijing, China, from 2016 to 2019
This study aimed to learn the epidemiological characteristics of 39 GI.6[P11] NoV outbreaks in Beijing, China, from 2016 to 2019 and to analyze the genetic diversity and phylogenetic process of GI.6[P11] strains. The Bayesian phylogenetic analysis of partial VP1 and RNA-dependent RNA polymerase (RdRp) genes showed that GI.6[P11] strains were clustered into four subclades. Eleven whole genome sequences were obtained through the amplicon sequencing with 16 pairs of newly designed primers. The phylogenetic trees based on the whole genome and ORF1, 2, and 3 showed that the clustering of the 11 strains was consistent with that ...
Source: Infection, Genetics and Evolution - August 19, 2023 Category: Genetics & Stem Cells Authors: Jiamei Fu Lingyu Shen Weihong Li Hanqiu Yan Baiwei Liu Yu Wang Yi Tian Lei Jia Quanyi Wang Daitao Zhang Zhiyong Gao Source Type: research
Genotypic diversity and recombination of norovirus GI.6[P11] associated acute gastroenteritis outbreaks in Beijing, China, from 2016 to 2019
This study aimed to learn the epidemiological characteristics of 39 GI.6[P11] NoV outbreaks in Beijing, China, from 2016 to 2019 and to analyze the genetic diversity and phylogenetic process of GI.6[P11] strains. The Bayesian phylogenetic analysis of partial VP1 and RNA-dependent RNA polymerase (RdRp) genes showed that GI.6[P11] strains were clustered into four subclades. Eleven whole genome sequences were obtained through the amplicon sequencing with 16 pairs of newly designed primers. The phylogenetic trees based on the whole genome and ORF1, 2, and 3 showed that the clustering of the 11 strains was consistent with that ...
Source: Infection, Genetics and Evolution - August 19, 2023 Category: Genetics & Stem Cells Authors: Jiamei Fu Lingyu Shen Weihong Li Hanqiu Yan Baiwei Liu Yu Wang Yi Tian Lei Jia Quanyi Wang Daitao Zhang Zhiyong Gao Source Type: research
Genotypic diversity and recombination of norovirus GI.6[P11] associated acute gastroenteritis outbreaks in Beijing, China, from 2016 to 2019
This study aimed to learn the epidemiological characteristics of 39 GI.6[P11] NoV outbreaks in Beijing, China, from 2016 to 2019 and to analyze the genetic diversity and phylogenetic process of GI.6[P11] strains. The Bayesian phylogenetic analysis of partial VP1 and RNA-dependent RNA polymerase (RdRp) genes showed that GI.6[P11] strains were clustered into four subclades. Eleven whole genome sequences were obtained through the amplicon sequencing with 16 pairs of newly designed primers. The phylogenetic trees based on the whole genome and ORF1, 2, and 3 showed that the clustering of the 11 strains was consistent with that ...
Source: Infection, Genetics and Evolution - August 19, 2023 Category: Genetics & Stem Cells Authors: Jiamei Fu Lingyu Shen Weihong Li Hanqiu Yan Baiwei Liu Yu Wang Yi Tian Lei Jia Quanyi Wang Daitao Zhang Zhiyong Gao Source Type: research
Genetic diversity and in silico analysis of Plasmodium knowlesi Serine Repeat Antigen (SERA) 3 antigen 2 in Malaysia
Infect Genet Evol. 2023 Aug 16:105490. doi: 10.1016/j.meegid.2023.105490. Online ahead of print.ABSTRACTPlasmodium knowlesi is the leading cause of malaria in Malaysia. Serine Repeat Antigens (SERAs) have an essential role in the parasite life cycle. However, genetic characterization on P. knowlesi SERA3 Ag2 (PkSERA3 Ag2) is lacking. In the present study, nucleotide diversity, natural selection, and haplotypes of PkSERA3 Ag2 in clinical samples from Peninsular Malaysia and Malaysian Borneo were investigated. A total of 50 P. knowlesi clinical samples were collected from Peninsular Malaysia and Malaysian Borneo. The PkSERA3...
Source: Infection, Genetics and Evolution - August 18, 2023 Category: Genetics & Stem Cells Authors: Jia Hui Tan Hans Xing Ding Mun Yik Fong Yee Ling Lau Source Type: research
Systematic analysis of prophages carried by Porphyromonas gingivalis
In conclusion, 26.7% P. gingivalis strains carry prophages, while the proportion of prophage genes in the P. gingivalis genome is relatively low. In addition, approximately 39.7% of the P. gingivalis prophage genes have ARGs identified, mainly against streptogramin, peptides, and aminoglycosides. Only a few prophages carry virulence genes. Prophages may play an important role in the acquisition, dissemination of antibiotic resistance genes, and pathogenicity evolution in P. gingivalis.PMID:37572952 | DOI:10.1016/j.meegid.2023.105489 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - August 12, 2023 Category: Genetics & Stem Cells Authors: B L Gu Y She G K Pei Y Du R Yang L X Ma Q Zhao S G Gao Source Type: research
Systematic analysis of prophages carried by Porphyromonas gingivalis
In conclusion, 26.7% P. gingivalis strains carry prophages, while the proportion of prophage genes in the P. gingivalis genome is relatively low. In addition, approximately 39.7% of the P. gingivalis prophage genes have ARGs identified, mainly against streptogramin, peptides, and aminoglycosides. Only a few prophages carry virulence genes. Prophages may play an important role in the acquisition, dissemination of antibiotic resistance genes, and pathogenicity evolution in P. gingivalis.PMID:37572952 | DOI:10.1016/j.meegid.2023.105489 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - August 12, 2023 Category: Genetics & Stem Cells Authors: B L Gu Y She G K Pei Y Du R Yang L X Ma Q Zhao S G Gao Source Type: research
Systematic analysis of prophages carried by Porphyromonas gingivalis
In conclusion, 26.7% P. gingivalis strains carry prophages, while the proportion of prophage genes in the P. gingivalis genome is relatively low. In addition, approximately 39.7% of the P. gingivalis prophage genes have ARGs identified, mainly against macrolides, peptides, and aminoglycosides. Only a few prophages carry virulence genes. Prophages may play an important role in the acquisition, dissemination of antibiotic resistance genes, and pathogenicity evolution in P. gingivalis.PMID:37572952 | DOI:10.1016/j.meegid.2023.105489 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - August 12, 2023 Category: Genetics & Stem Cells Authors: B L Gu Y She G K Pei Y Du R Yang L X Ma Q Zhao S G Gao Source Type: research
Systematic analysis of prophages carried by Porphyromonas gingivalis
In conclusion, 26.7% P. gingivalis strains carry prophages, while the proportion of prophage genes in the P. gingivalis genome is relatively low. In addition, approximately 39.7% of the P. gingivalis prophage genes have ARGs identified, mainly against macrolides, peptides, and aminoglycosides. Only a few prophages carry virulence genes. Prophages may play an important role in the acquisition, dissemination of antibiotic resistance genes, and pathogenicity evolution in P. gingivalis.PMID:37572952 | DOI:10.1016/j.meegid.2023.105489 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - August 12, 2023 Category: Genetics & Stem Cells Authors: B L Gu Y She G K Pei Y Du R Yang L X Ma Q Zhao S G Gao Source Type: research
Systematic analysis of prophages carried by Porphyromonas gingivalis
In conclusion, 26.7% P. gingivalis strains carry prophages, while the proportion of prophage genes in the P. gingivalis genome is relatively low. In addition, approximately 39.7% of the P. gingivalis prophage genes have ARGs identified, mainly against macrolides, peptides, and aminoglycosides. Only a few prophages carry virulence genes. Prophages may play an important role in the acquisition, dissemination of antibiotic resistance genes, and pathogenicity evolution in P. gingivalis.PMID:37572952 | DOI:10.1016/j.meegid.2023.105489 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - August 12, 2023 Category: Genetics & Stem Cells Authors: B L Gu Y She G K Pei Y Du R Yang L X Ma Q Zhao S G Gao Source Type: research
Systematic analysis of prophages carried by Porphyromonas gingivalis
In conclusion, 26.7% P. gingivalis strains carry prophages, while the proportion of prophage genes in the P. gingivalis genome is relatively low. In addition, approximately 39.7% of the P. gingivalis prophage genes have ARGs identified, mainly against macrolides, peptides, and aminoglycosides. Only a few prophages carry virulence genes. Prophages may play an important role in the acquisition, dissemination of antibiotic resistance genes, and pathogenicity evolution in P. gingivalis.PMID:37572952 | DOI:10.1016/j.meegid.2023.105489 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - August 12, 2023 Category: Genetics & Stem Cells Authors: B L Gu Y She G K Pei Y Du R Yang L X Ma Q Zhao S G Gao Source Type: research
