Biogenic Synthesis of Silver Nanoparticles Using Phyllanthus emblica Fruit Extract and Its Inhibitory Action Against the Pathogen Acidovorax oryzae Strain RS-2 of Rice Bacterial Brown Stripe
Conclusion
In conclusion, this study clearly provides an economical, environmental friendly, and straightforward reproducible approach in AgNPs synthesis employing P. Emblica fruit extracts as a reducing, stabilizing, and capping agent. The biosynthesized AgNPs were characterized thoroughly by UV-Visible and Energy Dispersive X-Ray Spectroscopy, X-Ray diffraction, FTIR, TEM, HRTEM and SEM. The FTIR results found several phytochemicals responsible for the rapid reduction of ions, leading to AgNPs formation. Especially, hydroxyl groups oxidation of hydrolysate, which likely stimulated the formation of nanoparticles. In the ...
Source: Frontiers in Microbiology - April 25, 2019 Category: Microbiology Source Type: research
A real-time loop mediated isothermal amplification assay for molecular detection of Burkholderia mallei, the aetiological agent of a zoonotic and re-emerging disease glanders.
Abstract
Burkholderia mallei, a potential biological warfare agent, is the causative agent of an infectious, fatal, and zoonotic disease, called glanders. Accurate and early diagnosis of glanders is important to control the disease lethality and infection spread. Molecular detection of B. mallei is considered strenuous because B. mallei is a subtractive genomic clone of B. pseudomallei. The present study was aimed at development of a real-time LAMP assay for detection of B. mallei. The LAMP assay was highly sensitive and could detect ≥250 fg of genomic DNA of B. mallei and ≥100 copies of recombinant ...
Source: Acta Tropica - April 4, 2019 Category: Infectious Diseases Authors: Saxena A, Pal V, Tripathi NK, Goel AK Tags: Acta Trop Source Type: research
Cover
The cover image is based on the Original ResearchAn increase in intracellular p62/NBR1 and persistence of Burkholderia mallei and B. pseudomallei in infected mice linked to autophagy deficiency by Kamal U. Saikh et al., DOI:10.1002/iid3.239. (Source: Immunity, Inflammation and Disease)
Source: Immunity, Inflammation and Disease - March 13, 2019 Category: Allergy & Immunology Authors: Kamal U. Saikh,
Jennifer L. Dankmeyer,
Xiankun Zeng,
Robert G. Ulrich,
Kei Amemiya Tags: COVER Source Type: research
An increase in intracellular p62/NBR1 and persistence of Burkholderia mallei and B. pseudomallei in infected mice linked to autophagy deficiency
ConclusionTaken together, our results suggest that an increase in intracellular p62/NBR1 may be a potential host cell biomarker ofB. mallei orB. pseudomallei infections, and identifying autophagy manipulation may potentially aid to therapeutic approach for complete clearance of the pathogen. (Source: Immunity, Inflammation and Disease)
Source: Immunity, Inflammation and Disease - December 19, 2018 Category: Allergy & Immunology Authors: Kamal U. Saikh,
Jennifer L. Dankmeyer,
Xiankun Zeng,
Robert G. Ulrich,
Kei Amemiya Tags: ORIGINAL RESEARCH Source Type: research
Rapid antimicrobial susceptibility testing and β-lactam-induced cell morphology changes of Gram-negative biological threat pathogens by optical screening
For Yersinia pestis, Burkholderia pseudomallei, and Burkholderia mallei, conventional broth microdilution (BMD) is considered the gold standard for antimicrobial susceptibility testing (AST) and, depending on the... (Source: BMC Microbiology)
Source: BMC Microbiology - December 18, 2018 Category: Microbiology Authors: Heather P. McLaughlin and David Sue Tags: Research article Source Type: research
Burkholderia mallei: the dynamics of networks and disease transmission
This article is protected by copyright. All rights reserved. (Source: Transboundary and Emerging Diseases)
Source: Transboundary and Emerging Diseases - November 14, 2018 Category: Veterinary Research Authors: Nicol ás Céspedes Cárdenas,
Jason Onell Ardila Galvis,
Alicia Appel Farinati,
José Henrique Hildebrand Grisi‐Filho,
Gustavo Nogueira Diehl,
Gustavo Machado Tags: Original Article Source Type: research
Improvement of recombinant-truncated Burkholderia motility protein A (BimA)-based indirect ELISA for equine glanders.
Authors: Singh S, Dohre SK, Kamthan A, Pal V, Karothia BS, Singha HS, Kumar S
Abstract
Glanders is a contagious and highly fatal disease of equines with zoonotic potential. It is caused by a Gram-negative, nonmotile bacterium Burkholderia mallei. Complement fixation test (CFT) is one of the most commonly used tests for diagnosis of glanders; however, it has some limitations. A recombinant-truncated Burkholderia intracellular motility A (BimA) protein-based indirect enzyme-linked immunosorbent assay (iELISA) was previously reported by us for glanders diagnosis, which has been re-optimized in this study usin...
Source: Journal of Immunoassay and Immunochemistry - October 13, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research
High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei.
In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.
PMID: 29751195 [PubMed - as supplied by publisher] (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - May 8, 2018 Category: Genetics & Stem Cells Authors: Girault G, Wattiau P, Saqib M, Martin B, Vorimore F, Singha H, Engelsma M, Roest HJ, Spicic S, Grunow R, Vicari N, De Keersmaecker SCJ, Roosens NHC, Fabbi M, Tripathi BN, Zientara S, Madani N, Laroucau K Tags: Infect Genet Evol Source Type: research
Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms [Bacteriology]
Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-plate formic acid, and ethanol/formic acid tube extraction methods, were evaluated for their ability to render highly pathogenic organisms nonviable and safe for handling in a biosafety level 2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight-laboratory study to examine the u...
Source: Journal of Clinical Microbiology - November 27, 2017 Category: Microbiology Authors: Rudrik, J. T., Soehnlen, M. K., Perry, M. J., Sullivan, M. M., Reiter-Kintz, W., Lee, P. A., Pettit, D., Tran, A., Swaney, E. Tags: Bacteriology Source Type: research
First molecular characterisation of a Brazilian Burkholderia mallei strain isolated from a mule in 2016.
We present the first molecular characterisation based on MLVA and SNP analysis of a strain of Burkholderia mallei isolated from a mule found dead in Brazil in 2016.
PMID: 29146548 [PubMed - as supplied by publisher] (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - November 13, 2017 Category: Genetics & Stem Cells Authors: Laroucau K, Lucia de Assis Santana V, Girault G, Martin B, Miranda da Silveira PP, Brasil Machado M, Joseph M, Wernery R, Wernery U, Zientara S, Madani N Tags: Infect Genet Evol Source Type: research
Quorum Sensing in Burkholderia pseudomallei and Other Burkholderia species
AbstractPurpose of ReviewBurkholderia pseudomallei andB.mallei are the etiological agents of severe diseases melioidosis and glanders, respectively, and are recalcitrant to many antibiotics. This review highlights recent findings on the quorum sensing (QS) systems of these pathogens and a closely related speciesB.thailandensis.Recent FindingsQuorum-sensing mechanisms have garnered considerable interest due to their ability to regulate bacterial physiology and metabolism. Research intoBurkholderia spp. has led to significant findings that delineate the important role of these redundant, yet distinct, QS pathways in bacteria...
Source: Current Tropical Medicine Reports - September 15, 2017 Category: Tropical Medicine Source Type: research
Development of a TaqMan real-time polymerase chain reaction assay for detection of Burkholderia mallei
Burkholderia mallei is the causative agent of glanders, a highly contagious and re-emerging zoonotic disease. B. mallei is closely related to B. pseudomallei, the etiological agent of melioidosis. The genetic proximity between B. mallei and B. pseudomallei had always been a problem in development of specific molecular detection tests for B. mallei. In present investigation, we describe a rapid, sensitive and specific TaqMan real-time polymerase chain reaction (PCR) assay for detection of B. mallei. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - August 14, 2017 Category: Veterinary Research Authors: Vijai Pal, Apoorva Saxena, Sandeep Singh, Subodh Kumar, Ajay Kumar Goel Source Type: research
Identification of a new diagnostic antigen for glanders using immunoproteome analysis.
Abstract
Glanders is a disease of horses, donkeys and mules. The causative agent Burkholderia mallei, is a biorisk group 3 pathogen and is also a biothreat agent. Simple and rapid diagnostic tool is essential for control of glanders. Using a proteomic approach and immunoblotting with equine sera, we identified 12 protein antigens that may have diagnostic potential. Various immunoreactive proteins e.g. GroEL, translation elongation factor Tu, elongation factor Ts, arginine deiminase, malate dehydrogenase, DNA directed RNA polymerase subunit alpha were identified on 2-dimentional immunoblots. One of these pr...
Source: Comparative immunology, microbiology and infectious diseases. - July 29, 2017 Category: Allergy & Immunology Authors: Dohre SK, Kamthan A, Singh S, Alam SI, Kumar S Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Seroprevalence and risk factors of glanders in working equines - findings of a cross-sectional study in Punjab province of Pakistan.
Abstract
Glanders is an infectious and contagious bacterial disease of equines. A little is known about its seroprevalence and risk factors in working equines in countries where the disease is endemic. Also, there are no reports on prevalence of the disease in areas where there is a prior evidence of Burkholderia (B.) mallei detection in soil. A cross-sectional study was conducted in selected districts (n=09) of Punjab province of Pakistan during 2014-2015. A total of 1008 serum samples were screened for detection of antibodies to B. mallei with complement fixation test followed by western blot. The overal...
Source: Acta Tropica - July 28, 2017 Category: Infectious Diseases Authors: Ghori MT, Khan MS, Khan JA, Rabbani M, Shabbir MZ, Chaudhry HR, Ali MA, Muhammad J, Elschner MC, Jayarao BM Tags: Acta Trop Source Type: research
Antibodies against In Vivo-Expressed Antigens Are Sufficient To Protect against Lethal Aerosol Infection with Burkholderia mallei and Burkholderia pseudomallei [Host Response and Inflammation]
Burkholderia mallei, a facultative intracellular bacterium and tier 1 biothreat, causes the fatal zoonotic disease glanders. The organism possesses multiple genes encoding autotransporter proteins, which represent important virulence factors and targets for developing countermeasures in pathogenic Gram-negative bacteria. In the present study, we investigated one of these autotransporters, BatA, and demonstrate that it displays lipolytic activity, aids in intracellular survival, is expressed in vivo, elicits production of antibodies during infection, and contributes to pathogenicity in a mouse aerosol challenge model. A mut...
Source: Infection and Immunity - July 19, 2017 Category: Infectious Diseases Authors: Zimmerman, S. M., Dyke, J. S., Jelesijevic, T. P., Michel, F., Lafontaine, E. R., Hogan, R. J. Tags: Host Response and Inflammation Source Type: research
