Activation of Toll-Like Receptors by Live Gram-Negative Bacterial Pathogens Reveals Mitigation of TLR4 Responses and Activation of TLR5 by Flagella
In conclusion, we have uncovered new properties of the Gram-negative pathogens, and their interaction with TLRs of the host. Further studies are needed to include other microorganism to extend our observations with their interaction with TLRs, and to the possibility of leading to new efforts in therapeutics against these pathogens. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - November 23, 2021 Category: Microbiology Source Type: research
Methodological tools to study species of the genus Burkholderia
Appl Microbiol Biotechnol. 2021 Nov 10:1-16. doi: 10.1007/s00253-021-11667-3. Online ahead of print.ABSTRACTBacteria belonging to the Burkholderia genus are extremely versatile and diverse. They can be environmental isolates, opportunistic pathogens in cystic fibrosis, immunocompromised or chronic granulomatous disease patients, or cause disease in healthy people (e.g., Burkholderia pseudomallei) or animals (as in the case of Burkholderia mallei). Since the genus was separated from the Pseudomonas one in the 1990s, the methodological tools to study and characterize these bacteria are evolving fast. Here we reviewed the tec...
Source: Applied Microbiology and Biotechnology - November 10, 2021 Category: Microbiology Authors: Viola Camilla Scoffone Gabriele Trespidi Giulia Barbieri Samuele Irudal Aygun Israyilova Silvia Buroni Source Type: research
Interactions Between Pathogenic Burkholderia and the Complement System: A Review of Potential Immune Evasion Mechanisms
The genus Burkholderia contains over 80 different Gram-negative species including both plant and human pathogens, the latter of which can be classified into one of two groups: the Burkholderia pseudomallei complex (Bpc) or the Burkholderia cepacia complex (Bcc). Bpc pathogens Burkholderia pseudomallei and Burkholderia mallei are highly virulent, and both have considerable potential for use as Tier 1 bioterrorism agents; thus there is great interest in the development of novel vaccines and therapeutics for the prevention and treatment of these infections. While Bcc pathogens Burkholderia cenocepacia, Burkholderia multivoran...
Source: Frontiers in cellular and infection microbiology - September 30, 2021 Category: Microbiology Source Type: research
Molecular epidemiology of Burkholderia mallei isolates from India (2015-2016): New SNP markers for strain tracing
In this study, 10 B. mallei strains isolated from four states in India during 2015-2016 were whole genome sequenced; SNP analysis further confirmed their position in the L2B2sB2 branch. To better track the strains, four new markers targeting Indian or Pakistani strains, and specifically targeting sub-groups within the Indian strains, were identified. The new SNP markers were tested and validated on the 10 Indian isolates included in this study as well as on 6 contemporary B. mallei Pakistani strains. These rapid and discriminating typing tools will contribute to the epidemiological monitoring of B. mallei infections, parti...
Source: Infection, Genetics and Evolution - September 3, 2021 Category: Genetics & Stem Cells Authors: H Singha F Vorimore Sheetal Saini T Deshayes M Saqib B N Tripathi K Laroucau Source Type: research
Validation of three qPCR for the detection of Burkholderia mallei in equine tissue samples
Arch Microbiol. 2021 May 24. doi: 10.1007/s00203-021-02367-7. Online ahead of print.ABSTRACTBurkholderia mallei is the causative agent of glanders, a zoonosis listed by the World Organization for Animal Health as of mandatory notification. In this work, a comparison of three qPCR protocols was made, two of them based on articles by other authors and one standardized in house, this last one aiming at a genomic region that does not exist in other species of the Burkholderia genus. All qPCRs showed high efficiency and good repeatability. However, reactions with Cq between 36 and 40 were considered suspicious and unreliable, r...
Source: Archives of Microbiology - May 24, 2021 Category: Microbiology Authors: Ant ônio Augusto Fonseca Júnior Carla Amaral Pinto Cid Arist óteles de Siqueira Alencar Bruna Lopes Bueno Jenner K Pimenta Dos Reis Maur ício Baltazar de Carvalho Filho Source Type: research
Validation of three qPCR for the detection of Burkholderia mallei in equine tissue samples
Arch Microbiol. 2021 May 24. doi: 10.1007/s00203-021-02367-7. Online ahead of print.ABSTRACTBurkholderia mallei is the causative agent of glanders, a zoonosis listed by the World Organization for Animal Health as of mandatory notification. In this work, a comparison of three qPCR protocols was made, two of them based on articles by other authors and one standardized in house, this last one aiming at a genomic region that does not exist in other species of the Burkholderia genus. All qPCRs showed high efficiency and good repeatability. However, reactions with Cq between 36 and 40 were considered suspicious and unreliable, r...
Source: Archives of Microbiology - May 24, 2021 Category: Microbiology Authors: Ant ônio Augusto Fonseca Júnior Carla Amaral Pinto Cid Arist óteles de Siqueira Alencar Bruna Lopes Bueno Jenner K Pimenta Dos Reis Maur ício Baltazar de Carvalho Filho Source Type: research
Molecular Typing of Burkholderia mallei Isolates from Equids with Glanders, India
Emerg Infect Dis. 2021 Jun;27(6):1745-1748. doi: 10.3201/eid2706.203232.ABSTRACTWe collected 10 Burkholderia mallei isolates from equids in 9 districts in India during glanders outbreaks in 2013-2016. Multilocus variable-number tandem-repeat analysis showed 7 outbreak area-related genotypes. The study highlights the utility of this analysis for epidemiologically tracing of specific B. mallei isolates during outbreaks.PMID:34013856 | DOI:10.3201/eid2706.203232 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - May 20, 2021 Category: Infectious Diseases Authors: Harisankar Singha Mandy C Elschner Praveen Malik Sheetal Saini Bhupendra N Tripathi Katja Mertens-Scholz Hanka Brangsch Falk Melzer Raj K Singh Heinrich Neubauer Source Type: research
Molecular Typing of Burkholderia mallei Isolates from Equids with Glanders, India
H. Singha et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - May 6, 2021 Category: Infectious Diseases Source Type: research
Comparison of three non-human primate aerosol models for glanders, caused by Burkholderia mallei
Publication date: Available online 27 April 2021Source: Microbial PathogenesisAuthor(s): David M. Waag, Taylor B. Chance, Sylvia R. Trevino, Franco D. Rossi, David P. Fetterer, Kei Amemiya, Jennifer L. Dankmeyer, Susham S. Ingavale, Steven A. Tobery, Xiankun Zeng, Steven J. Kern, Patricia L. Worsham, Christopher K. Cote, Susan L. Welkos (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 28, 2021 Category: Infectious Diseases Source Type: research
