Molecular detection of Hsp90 inhibitor suppressing PCV2 replication in host cells
Publication date: Available online 25 April 2019Source: Microbial PathogenesisAuthor(s): Jie Liu, Chang Ma, Xuliang Zhang, Jinwei You, Min Dong, Li Chen, Ping Jiang, Shifeng YunAbstractPorcine Circovirus Type 2 (PCV2) is a pathogen that has the ability to cause devastating disease manifestations in pig populations with major economic implications. Our previous research found that Hsp90 is required for PCV2 production in PK-15 and 3D4/31 cells. The aim of this study was to evaluate the effect of Hsp90 inhibitor regulating PCV2 replication and to explore its underlying mechanism. In PK-15 and 3D4/31 cells treated with 17...
Source: Microbial Pathogenesis - April 25, 2019 Category: Infectious Diseases Source Type: research

Transcriptomic analysis of small intestinal mucosa from porcine epidemic diarrhea virus infected piglets
Publication date: Available online 23 April 2019Source: Microbial PathogenesisAuthor(s): Pei Sun, Qarih Fahd, Yezhen Li, Yao Zhao, Jie Li, Majjid A. Qaria, Zhan Song He, Yuzhen Fan, Qiang Zhang, Qianming Xu, Zongjun Yin, Xingang Xu, Yu LiAbstractCaused by porcine epidemic diarrhea virus (PEDV), porcine epidemic diarrhea (PED) is an acute infectious disease which causes damage to the intestine including intestinal villus atrophy and shedding, leading to serious economic losses to the pig industry worldwide. In order to obtain detailed information about the pathogenesis and host immune response in a PEDV-infected host for fi...
Source: Microbial Pathogenesis - April 24, 2019 Category: Infectious Diseases Source Type: research

Cholinesterase's activities of infected mice by Brucella ovis
Publication date: Available online 24 April 2019Source: Microbial PathogenesisAuthor(s): Géssica Perin, Nathiele B. Bottari, Anielen D. Silva, Antonise M. Jaguezeski, Teane M.A. Gomes, Thalison F. Lopes, Maria Rosa C. Schetinger, Vera M. Morsch, Aleksandro S. Da SilvaAbstractThe role of cholinesterase in inflammatory reactions has been described in several infectious diseases. However, in Brucella spp. this has not yet been studied. Therefore, the objective of this study was to evaluate whether experimental infection by Brucella ovis alters the cholinergic activity in pro- or anti-inflammatory responses to the disea...
Source: Microbial Pathogenesis - April 24, 2019 Category: Infectious Diseases Source Type: research

Antioxidant, antibacterial, and anti-adhesive activities of biosurfactants isolated from Bacillus strains
Publication date: Available online 24 April 2019Source: Microbial PathogenesisAuthor(s): Sib Sankar Giri, EunChae Ryu, Venkatachalam Sukumaran, Se Chang ParkAbstractBiosurfactants are surface-active compounds that display a range of physiological functions. The present study investigated the antioxidant, antimicrobial, and anti-adhesive or anti-biofilm potential of biosurfactants isolated from Bacillus subtilis VSG4 and Bacillus licheniformis VS16. The antioxidant activity of the biosurfactants was studied in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals. At 5 mg/mL of the biosurfactant concentra...
Source: Microbial Pathogenesis - April 24, 2019 Category: Infectious Diseases Source Type: research

Genotyping of long term persistent Staphylococcus aureus in bovine subclinical mastitis
Publication date: Available online 20 April 2019Source: Microbial PathogenesisAuthor(s): B.F. Rossi, E.C.R. Bonsaglia, I.G. Castilho, S.T.A. Dantas, A. Salina, H. Langoni, J.C.F. Pantoja, P.E. Budri, D. Fitzgerald-Hughes, A. Fernandes Júnior, V.L.M. RallAbstractBovine mastitis affects dairy cattle worldwide and Staphylococcus aureus is one of the most common microorganisms involved in subclinical and chronic disease. Superantigens, such as enterotoxins contribute to S. aureus persistence and pathogenicity in this disease. Subclinical and chronic mastitis cases were diagnosed and S. aureus isolates from sub-clinical ...
Source: Microbial Pathogenesis - April 21, 2019 Category: Infectious Diseases Source Type: research

Probiotic potential of Weissella strains isolated from horse feces, a probable equine probiotic
In this study, we isolated four Weissella confusa strains from the healthy horse feces to test their potential as equine probiotics. The identification and characteristics of these isolates were determined as per standard methods. Resistance and susceptibility of the isolated strains were tested to low pHs, different heat treatments, commonly used antibiotics and against the pathogenic strains of Salmonella, Pasteurella, Staphylococcus aureus, and Escherichia coli. After 3 h cultural in different PH medium, the 4 strains still had a certain amount of survival above pH 3.0. WH2 and WH4 were still viable at pH2.5. All the ...
Source: Microbial Pathogenesis - April 20, 2019 Category: Infectious Diseases Source Type: research

Immunogenic evaluation of FMD virus immuno-dominant epitopes coupled with IL-2/FcIgG in BALB/c mice
In this study, we produced epitope recombinant vaccines from the VP1 protein of the FMD virus for serotype O of Iran that conjugated with Fc Immunoglobulin (FcIgG) and Interleukin-2 (IL-2). Multiple-epitope constructs included Polytope, Polytope-IL2-FcIgG, Polytope-IL2, Polytope-FcIgG that cloned and expressed in E. coli BL21 (DE3).To evaluate whether these epitope recombinant vaccines induce immune responses, BALB/c mice were injected with the epitope recombinant vaccines and their immune responses were compared with a negative control group. The humoral and cellular immune responses were measured by ELISA. The results sh...
Source: Microbial Pathogenesis - April 18, 2019 Category: Infectious Diseases Source Type: research

Immunization of mice by the co-administration of codon-optimized HPV16 E7 and lL12 genes against HPV16-associated cervical cancer
ConclusionThe co-administration of codon-optimized HPV16 E7 gene with IL12 significantly enhances the DNA vaccine potency against HPV16-associated cervical cancer. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 18, 2019 Category: Infectious Diseases Source Type: research

L. pseudomesenteroides and L. johnsonii isolated from yaks in Tibet modulate gut microbiota in mice to ameliorate enteroinvasive Escherichia coli-induced diarrhea
In conclusion, pre-supplementation of LP1 and LJ1 can mitigate EIEC-induced intestinal flora dysbiosis and can also reduce EIEC-associated diarrhea. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Canine distemper virus increased the differentiation of CD4+CD8+ T cells and mRNA expression of inflammatory cytokines in peripheral blood lymphocyte from canine
ConclusionsAfter CDV infection, PBL differentiated into CD4+CD8+ T cells and initiated inflammatory response. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Association between the mannose-binding lectin (MBL)-2 gene variants and serum MBL with pulmonary tuberculosis: An update meta-analysis and systematic review
This study suggested that the MBL-2 gene polymorphisms may be involved in the pathogenesis of PTB, and serum MBL may be a biomarker for the diagnosis of PTB. More rigorous research is needed in the future to confirm these findings further. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Comparison of MALDI-TOF MS Biotyper and 16S rDNA sequencing for identification of Pseudomonas species isolated from fish
Publication date: Available online 15 April 2019Source: Microbial PathogenesisAuthor(s): Miroslava Kačániová, Alīna Klūga, Attila Kántor, Juraj Medo, Jana Žiarovská, Czeslaw Puchalski, Margarita TerentjevaAbstractEffective and reliable methods of identification of Pseudomonas spp. are important for characterization of microorganisms. The aim of present study was to compare identification conducted with MALDI-TOF MS Biotyper and 16S rDNA sequencing of Pseudomonas species isolated from fish. Altogether, 13 different Pseudomonas species were isolated from freshwater fish. Phylogenetic analysis ...
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Conjugal transfer of PMQR from uropathogenic E.coli under high ciprofloxacin selection pressure generates gyrA mutation
Publication date: Available online 16 April 2019Source: Microbial PathogenesisAuthor(s): Shreya Basu, Mandira MukherjeeAbstractIncidence of high fluoroquinolone resistance has been rising rapidly worldwide. Resistance against fluoroquinolones can be either chromosomal or plasmid mediated. Plasmid mediated quinolone resistant(PMQR) genes impart low level of resistance against fluoroquinolones but provides favorable background for selection of additional chromosomally encoded resistance mechanisms. In the natural habitat, conjugal transfer of PMQR genes provides a vehicle for dissemination of fluoroquinolone resistance. Our ...
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Staphylococcus aureus versus neutrophil: Scrutiny of ancient combat
Publication date: Available online 16 April 2019Source: Microbial PathogenesisAuthor(s): Ahmad Nasser, Parham Jazireian, Hossein Safari, Mahmoud Alizade-Sani, Mohammad Reza Pourmand, Taher AzimiAbstractStaphylococcus aureus (S.aureus) is a Gram-positive bacterium that causes many infections and diseases. This pathogen can cause many types of infections such as impetigo, toxic shock syndrome toxin (TSST1), pneumonia, endocarditis, and autoimmune diseases like lupus erythematosus and can infect other healthy individuals. In the pathogenic process, colonization is a main risk factor for invasive diseases. Various factors incl...
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Baicalin tetrazole acts as anti-pneumocystis carinii pneumonia candidate in immunosuppressed rat model
Publication date: Available online 16 April 2019Source: Microbial PathogenesisAuthor(s): Lin Chen, Xin Zhang, Xiaying Peng, Yang Yang, Hua YuAbstractThe present study was aimed to synthesize and evaluate tetrazoles of baicalin against Pneumocystis carinii pneumonia in the rat model. Among the seven synthesized baicalin tetrazoles, one with trifloromethyl group in the aromatic ring was found to be most potent during the initial study. The mechanism of preventive effect of most potent compound 4c against Pneumocystis carinii pneumonia was investigated in detail. The compound 4c decreased the parasitic load by almost 99% in t...
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Biosynthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from leaf extract of Mentha pulegium (L.)
Publication date: Available online 17 April 2019Source: Microbial PathogenesisAuthor(s): Samira Shahriyari Rad, Ali Mohamadi Sani, Sharareh MohseniAbstractThe synthesis of metal and semiconductor nanoparticles is an expanding research area due to the potential applications in the development of novel technologies. In the present study, a simple and eco-friendly synthesis of zinc oxide nanoparticles (ZnO NPs) using leaf extract of Mentha pulegium L. has been used. The biosynthesized ZnO NPs were characterized various techniques such as UV–Vis absorption spectroscopy, X-ray diffraction (XRD), field emission scanning el...
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Characterization, high production and antimicrobial activity of exopolysaccharides from Lactococcus lactis F-mou
Publication date: Available online 17 April 2019Source: Microbial PathogenesisAuthor(s): Fatima Nehal, Mouna Sahnoun, Slim Smaoui, Bassem Jaouadi, Samir Bejar, Sbaihia MohammedAbstractA new exopolysaccharide (EPS) was produced by the Lactococcus lactis F-mou strain (LT898177.1) isolated from the Sahrawi camel milk in the Bir-Naam region, Algeria. The most influential production parameters were screened by the Plackett-Burman design for enhancing EPS yield utilizing the Mech-Degla juice as a low-cost raw material. An optimum condition of a 0.49 of inoculum size, a 100 rpm of agitation rate, and a 12 h of incubation peri...
Source: Microbial Pathogenesis - April 17, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: May 2019Source: Microbial Pathogenesis, Volume 130Author(s): (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 14, 2019 Category: Infectious Diseases Source Type: research

The regulatory role of C1q on Helicobacter pylori-induced inflammatory cytokines secretion in THP-1 cells
Publication date: Available online 12 April 2019Source: Microbial PathogenesisAuthor(s): Zhang Ying, Li Jiaojiao, Rong Qianyu, Xu Zheng, Ding Yunfei, Cao Qizhi, Ji Xiaofei, Zhao Huilin, Yulong Wu, Li BoqingAbstractC1q, as a LAIR-1 ligand, maintains monocytes quiescence and possess immunosuppressive properties. To understand the roles and molecular mechanisms, C1q mediated inflammation cytokines and several pivotal proteins in THP-1 cells after H. pylori infection were detected. The results showed that the expression of IL-8, IL-10, LAIR-1, phosphorylated/total JNK, phosphorylated/total p38-MAPK, phosphorylated/total AKT ...
Source: Microbial Pathogenesis - April 14, 2019 Category: Infectious Diseases Source Type: research

Influences of stress hormones on microbial infections
Publication date: Available online 11 April 2019Source: Microbial PathogenesisAuthor(s): Emmanuel Konadu Sarkodie, Muhammad Naveed, Ali Asghar Mirjat, Muhammad Saeed, Shuxin Zhou, Mirza Muhammad Faran Ashraf Baig, Sarah Ama Baidoo, Awais Ullah Ihsan, Kalyan Bikram Shah, Abdullahi Ayuba, Daniel Chikere Ali, Weihua ChuAbstractStress hormones have been recently suggested to influence the pathogenicity of bacteria significantly. Stress has been identified as part of the factors causing an outbreak of infections in the aquaculture industry. The most studied neuroendocrine hormonal family from a microbial endocrinology perspecti...
Source: Microbial Pathogenesis - April 12, 2019 Category: Infectious Diseases Source Type: research

Attenuated Listeria monocytogenes protecting zebrafish (Danio rerio) against Vibrio species challenge
Publication date: Available online 12 April 2019Source: Microbial PathogenesisAuthor(s): Chengchao Ding, Qing Liu, Jie Li, Junfei Ma, Shuying Wang, Qingli Dong, Dongpo Xu, Jingxuan Qiu, Xiang WangAbstractLive attenuated bacteria is a promising candidate vector for the delivery of vaccines in clinic trials. In the field of aquaculture industry, live vector vaccine also could provide long-term and effective protection against fish bacterial diseases. In our previous work, we demonstrated attenuated Listeria monocytogenes (Lm) had the potential to be an aquaculture vaccine vector in cellular level and zebrafish model. To furt...
Source: Microbial Pathogenesis - April 12, 2019 Category: Infectious Diseases Source Type: research

Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa
Publication date: Available online 10 April 2019Source: Microbial PathogenesisAuthor(s): Dušan Ušjak, Branka Ivković, Dragana D. Božić, Lidija Bošković, Marina MilenkovićAbstractAcinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones a...
Source: Microbial Pathogenesis - April 11, 2019 Category: Infectious Diseases Source Type: research

Transcriptional regulation of Yersinia pestis biofilm formation
Publication date: Available online 10 April 2019Source: Microbial PathogenesisAuthor(s): Lei Liu, Shangen ZhengAbstractYersinia pestis, the causative agent of plague, is transmitted primarily by infected fleas in nature. Y. pestis can produce biofilms that block flea's proventriculus and promote flea-borne transmission. Transcriptional regulation of Y. pestis biofilm formation plays an important role in the response to complex changes in environments, including temperature, pH, oxidative stress, and restrictive nutrition conditions, and contributes to Y. pestis growth, reproduction, transmission, and pathogenesis. A set of...
Source: Microbial Pathogenesis - April 11, 2019 Category: Infectious Diseases Source Type: research

Generation of porcine Pasteurella multocida ghost vaccine and examination of its immunogenicity against virulent challenge in mice
Publication date: Available online 10 April 2019Source: Microbial PathogenesisAuthor(s): Xuhua Ran, Xiang-Zhu Meng, Hong-Li Geng, Chunlong Chang, Xiaohong Chen, Xiaobo Wen, Hongbo NiAbstractPasteurella multocida (PM) causes a varity of clinical manifestation in domestic animals, even acute death. Vaccination is among effective strategy to prevent and control PM-related diseases. Bacterial ghosts (BGs) are empty bacterial envelopes, which sustain subtle antigenic comformation in bacterial outer-membrane and exhibit higher efficacy compared to inactivated vaccines. Here, a BG vaccine generated from the porcine PM reference s...
Source: Microbial Pathogenesis - April 11, 2019 Category: Infectious Diseases Source Type: research

Oxidative stress and antioxidant responses in Nile tilapia Oreochromis niloticus experimentally infected by Providencia rettgeri
In this study, Nile tilapia Oreochromis niloticus was infected by Providencia rettgeri to evaluate the oxidative stress and antioxidant responses in the fish tissues. Juvenile Nile tilapia was divided into two groups, as follow: control (uninfected) and experimentally infected with 100 μL of P. rettgeri suspension containing 2.4 × 107 viable cells/fish, and the liver and kidney tissues were collected on days 7 and 14 post-infection (PI). Liver and kidney ROS and lipid peroxidation levels were high in infected fish on day 14 PI compared to control group, while superoxide dismutase activity was lower in liver ...
Source: Microbial Pathogenesis - April 11, 2019 Category: Infectious Diseases Source Type: research

Immunization with the ferric iron-binding periplasmic protein HitA provides protection against Pseudomonas aeruginosa in the murine infection model
This study focused on evaluation of the iron acquisition protein HitA as a potential vaccine candidate against P. aeruginosa in a murine infection model. The Recombinant ferric iron-binding periplasmic protein HitA was overexpressed in Escherichia coli and was purified using metal affinity chromatography. The purified antigen was administered to mice in combination with Bacillus Calmette–Guérin (BCG) as an adjuvant using different vaccination regimens. Serum samples were tested for IgG1, IgG2a and total IgG antibody responses which were extremely significant. Following challenge of mice with P. aeruginosa, the...
Source: Microbial Pathogenesis - April 11, 2019 Category: Infectious Diseases Source Type: research

In-vitro phagocytosis and intracellular killing of Pasteurella multocida B:2 by phagocytic cells of buffaloes
This study aims to evaluate the in-vitro efficacy of macrophages and neutrophils of buffalo following exposure to P. multocida B:2. In-vitro infections were done using 107 cfu/ml of P. multocida B:2 for Group 1, Escherichia coli for Group 2 and Mannhaemia haemolytica A:2 for Group 3 cells. The inoculated cell cultures were harvested at 0, 30, 60 and 120 min post-exposure and the phagocytic, killing and cell death rates were determined. Both phagocytosis and killing rates of all bacteria increased over time. Phagocytosis involved between 71% and 73% neutrophils and between 60% and 64% macrophages at 120 min. Killing...
Source: Microbial Pathogenesis - April 11, 2019 Category: Infectious Diseases Source Type: research

Horizontal transmission of Metarhizium anisopliae between Spoladea recurvalis (Lepidoptera: Crambidae) adults and compatibility of the fungus with the attractant phenylacetaldehyde
Publication date: Available online 10 April 2019Source: Microbial PathogenesisAuthor(s): Selpha Opisa, Hannalene du Plessis, Komivi S. Akutse, KomiK.M. Fiaboe, Sunday EkesiAbstractThe compatibility of the entomopathogenic fungus Metarhizium anisopliae ICIPE 30 which was proved to be pathogenic to adult Spoladea recurvalis, and phenylacetaldehyde (PAA) floral attractant for lepidopteran moths, was investigated under laboratory and field conditions through spatial and temporal separations. Horizontal transmission of M. anisopliae ICIPE 30 between adult S. recurvalis and the number of conidia picked up by a single moth from t...
Source: Microbial Pathogenesis - April 11, 2019 Category: Infectious Diseases Source Type: research

Evaluation of adherence and anti-infective properties of probiotic Lactobacillus fermentum strain 4-17 against Escherichia coli causing urinary tract infection in humans
In this study, Lactobacillus fermentum strain 4-17 was isolated from kashkineh, an Iranian cereal fermented food, and identified by sequencing its 16S rRNA gene using universal primers. Its probiotic features, including resistance to acid, bile tolerance, antibacterial activity, resistance to intestinal and gastric juices, and hydrophobicity were evaluated. The ability of this strain to adhere to human intestinal cells as well as the anti-adhesive effect of L. fermentum strain 4-17 against E. coli isolated from patients with urinary tract infection was investigated. L. fermentum strain 4-17 was capable of surviving at vari...
Source: Microbial Pathogenesis - April 9, 2019 Category: Infectious Diseases Source Type: research

Characterization of serine acetyltransferase (CysE) from methicillin-resistant Staphylococcus aureus and inhibitory effect of two natural products on CysE
Publication date: Available online 9 April 2019Source: Microbial PathogenesisAuthor(s): Changming Chen, Qiulong Yan, Mengxing Tao, Huaying Shi, Xiuyan Han, Liqiu Jia, Yukun Huang, Lizhe Zhao, Chao Wang, Xiaochi Ma, Yufang MaAbstractMethicillin-resistant Staphylococcus aureus (MRSA) is a major hospital-acquired infective pathogen that has developed resistance to many antibiotics. It is imperious to develop novel anti-MRSA drugs to control the emergence of drug resistance. The biosynthesis of cysteine in bacteria is catalyzed by CysE and CysK. CysE was predicted to be important for bacterial viability, it could be a potentia...
Source: Microbial Pathogenesis - April 9, 2019 Category: Infectious Diseases Source Type: research

Anti-staphylococcal activity of Syagrus coronata essential oil: Biofilm eradication and in vivo action on Galleria mellonela infection model
In conclusion, SCEO is an antibacterial agent against S. aureus strains with different resistance phenotypes and able to disturb biofilm architecture. Our results show SCEO as a potential candidate to drug development. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 7, 2019 Category: Infectious Diseases Source Type: research

Sodium butyrate ameliorates Corynebacterium pseudotuberculosis infection in RAW264.7 macrophages and C57BL/6 mice
In conclusion, NaB ameliorated CP infection in RAW264.7 macrophages and C57BL/6 mice, and these effects may be related to the modulation of sodC, spaC, pld, and cramp expression. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 7, 2019 Category: Infectious Diseases Source Type: research

Mosloflavone attenuates the quorum sensing controlled virulence phenotypes and biofilm formation in Pseudomonas aeruginosa PAO1: In vitro, in vivo and in silico approach
Publication date: Available online 5 April 2019Source: Microbial PathogenesisAuthor(s): Sairengpuii Hnamte, Paramanantham Parasuraman, SampathKumar Ranganathan, Dinakara Rao Ampasala, Dhanasekhar Reddy, RanjithN. Kumavath, Kitlangki Suchiang, Saswat Kumar Mohanty, Siddhardha BusiAbstractQuorum sensing (QS) is the cell density dependent communication network which coordinates the production of pathogenic determinants in majority of pathogenic bacteria. Pseudomonas aeruginosa causes hospital-acquired infections by virtue of its well-defined QS network. As the QS regulatory network in P. aeruginosa regulates the virulence det...
Source: Microbial Pathogenesis - April 7, 2019 Category: Infectious Diseases Source Type: research

Candida albicans cell wall mannoprotein synergizes with lipopolysaccharide to affect RAW264.7 proliferation, phagocytosis and apoptosis
In this report, we examined the effects of the C. albicans cell wall mannoprotein (MP) on macrophage immunity. The MTS assay was used to detect cell proliferation activity and neutral red staining to observe cell phagocytosis. The Griess method was used to detect NO secretion in culture supernatants and apoptosis of macrophages were determined with use of FITC-Annexin V and PI staining. mRNA and protein expressions of JAK2, STAT3, IL-1β, IL-6, TNF-α and iNOS in RAW264.7 cells were determined with use of RT-PCR and western blot. MP significantly promoted the proliferation of RAW264.7 cells, inhibited their ph...
Source: Microbial Pathogenesis - April 4, 2019 Category: Infectious Diseases Source Type: research

The lung microbiome, vitamin D, and the tuberculous granuloma: A balance triangle
Publication date: Available online 3 April 2019Source: Microbial PathogenesisAuthor(s): María Elvira Balcells, Noemí Yokobori, Bo-young Hong, John Corbett, Jorge CervantesAbstractMycobacterium tuberculosis (Mtb) has the extraordinary ability to persist for decades within within granulomas in the human host. This histopathological structures involved in both protection and pathogenesis, are subject to various influences from the host systemically and through micro-niche environments. Despite the fact that vitamin D (VD) has a key role in macrophage activation and mycobacterial clearance in the early stages of ...
Source: Microbial Pathogenesis - April 4, 2019 Category: Infectious Diseases Source Type: research

In vitro and in silico docking studies of antibacterial compounds derived from endophytic Penicillium setosum
In this study, a potent Penicillium setosum sp. nov. was explored for its detailed antibacterial action on Escherichia coli and Staphylococcus aureus through different in vitro and in silico assays. Fluorescence based viability assay determined increase in the number of dead cells in course of time with the continual exposure of extract during a 4 h period. Scanning electron micrographs reflect the distinguishable morphological changes in treated cells, namely shortening of size, bubbles, and blisters on the surface of E. coli, as well as open holes and deep craters on the surface of S. aureus, ultimately leading to rupt...
Source: Microbial Pathogenesis - April 3, 2019 Category: Infectious Diseases Source Type: research

Musa basjoo regulates the gut microbiota in mice by rebalancing the abundance of probiotic and pathogen
In this study, maturity of M. basjoo were divided into four levels. Results indicated that M. basjoo in level Ⅱ (with 35% maturity) represented the greatest increase in the growth in vitro of probiotics, Lactobacillus plantarum FMNP01 and Lactobacillus casei FMNP02. After feeding M. basjoo with the middle dose (2.67 g/kg·BW) to mice for 21 days, gut microbiota from mice feces was isolated and sequenced. Results of 16SrDNA sequencing showed that the scattered genera of gut microbiota were significantly gathered. The amounts of different pathogens were decreased, while probiotics such as genera Bacteroides and Ros...
Source: Microbial Pathogenesis - April 3, 2019 Category: Infectious Diseases Source Type: research

Isolation, characterization, and PCR-based molecular identification of a siphoviridae phage infecting Shigella dysenteriae
ConclusionvB-SdyS-ISF003 is a novel virulent T1virus phage and has the appropriate potential for being used in bio controlling of S. dysenteriae in different condition. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - April 2, 2019 Category: Infectious Diseases Source Type: research

Rhamnolipid-involved antibiotics combinations improve the eradication of Helicobacter pylori biofilm in vitro: A comparison with conventional triple therapy
Publication date: Available online 2 April 2019Source: Microbial PathogenesisAuthor(s): Xiaonan Chen, Pengyu Li, Yuanna Shen, Yiqing Zou, Gang Yuan, Haiyan HuAbstractAntibiotics resistance of H. pylori has been increasing constantly accompanied with decreasing clearance rate clinically, which is demonstrated to be closely related to biofilms with higher resistance than planktonic bacteria for the dense extracellular polymeric substances. Rhamnolipid (RHL) is proved to not only damage the structure of biofilm, but also potentially inhibit bacterial adhesion. To investigate if RHL could promote eradicating rate of the conven...
Source: Microbial Pathogenesis - April 2, 2019 Category: Infectious Diseases Source Type: research

Baicalein triazole prevents respiratory tract infection by RSV through suppression of oxidative damage
Publication date: Available online 31 March 2019Source: Microbial PathogenesisAuthor(s): Cuicui Zhang, Na Li, Fang NiuAbstractBaicalein, an isolate of secutellaria baicalensis is known for its anti-inflammatory activity. In the present study, 12-triazole derivatives of baicalein were synthesized and evaluated against RSV infected BEAS-2B cells in vitro and in mice model in vivo. The preventive effect of most active compound 5f against RSV infection was studied in detail. The compound 5f treatment increased IFN-β1 expression in BEAS-2B cells infected with RSV. In BEAS-2B cells treatment with compound 5f inhibited RSV-i...
Source: Microbial Pathogenesis - April 1, 2019 Category: Infectious Diseases Source Type: research

Antimicrobial resistance and virulence genes of Streptococcus isolated from dairy cows with mastitis in China
In conclusion, the issue of drug resistance of Streptococcus is still a great concern in cattle health in China. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - March 31, 2019 Category: Infectious Diseases Source Type: research

Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis
Publication date: Available online 30 March 2019Source: Microbial PathogenesisAuthor(s): Hanadi M. Alyami, Livia S. Finoti, Hellen S. Teixeira, Abdulelah Aljefri, Denis F. Kinane, Manjunatha R. BenakanakereAbstractPolymorphonuclear neutrophils (PMNs) are indispensable in fighting infectious microbes by adopting various antimicrobial strategies including phagocytosis and neutrophil extracellular traps (NETs). Although the role and importance of PMNs in periodontal disease are well established, the specific molecular mechanisms involved in NET formation are yet to be characterized. In the present study, we sought to determin...
Source: Microbial Pathogenesis - March 31, 2019 Category: Infectious Diseases Source Type: research

Study on Streptococcus agalactiae infection in Javanese medaka (Oryzias javanicus Bleeker, 1854) model
This study determines the median lethal dose, and describes the clinico-pathological changes and disease development following Streptococcus agalactiae infection in Javanese medaka model. Javanese medakas were infected with S. agalactiae via intraperitoneal (IP) from 104 to 108 CFU/mL, and immersion (IM) route from 103 to 107 CFU/mL. The LD50–240h and clinico-pathological changes of the fish was determined until 240 h post infection (hpi). Next, the disease development was determined for 96 hpi in the fish following IP and IM infection at 103 CFU/mL and 104 CFU/mL, respectively. The LD50–240h of S. ag...
Source: Microbial Pathogenesis - March 31, 2019 Category: Infectious Diseases Source Type: research

The protective role of autophagy in sepsis
Publication date: Available online 30 March 2019Source: Microbial PathogenesisAuthor(s): Ying Feng, Boyi Liu, Xiang Zheng, Li Chen, Wei Chen, Zhicheng FangAbstractSepsis is characterized by life-threatening organ dysfunction caused by a deregulated host response to infection. Autophagy is one of the innate immune defense mechanisms against microbial attack. Previous studies have demonstrated that autophagy is activated initially in sepsis, followed by a subsequent phase of impairment. A number of sepsis-related studies have shown that autophagy plays a protective role in multiple organ injuries partly by clearing pathogens...
Source: Microbial Pathogenesis - March 31, 2019 Category: Infectious Diseases Source Type: research

Immunogenic reactivity of recombinant PKF and AbOmpA proteins as serum resistance factors against sepsis of Acinetobacter baumannii
In this study IgG1, IgG2c, Total-IgG concentrations, survival rates and spleen bacterial loads were studied in C57/BL mice model according to PKF, AbOmpA and AbOmpA + PKF vaccine formulations. The findings showed significant raises of IgG2c and Total-IgG in all three vaccinated groups in comparison with the control group. Whereas, there were low concentrations of IgG1 in all immunization plans. Colony counts of mice spleen showed the bacterial load of PKF plan had the most decrease of bacterial load (DBL = 5 log10 CFU/g). Taken together, this evaluation indicated that PKF vaccination plan induced a po...
Source: Microbial Pathogenesis - March 29, 2019 Category: Infectious Diseases Source Type: research

Immunomodulatory molecules regulate adhesin gene expression in Staphylococcus aureus: Effect on bacterial internalization into bovine mammary epithelial cells
In conclusion, our data support the fact that fatty acids and cholecalciferol regulate adhesin gene expression as well as bacteria internalization in nonprofessional phagocytic cells, which may lead to development of anti-virulence agents for control of pathogens. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - March 29, 2019 Category: Infectious Diseases Source Type: research

Peripheral blood and hepatic Toll-like receptor 7 expression and interferon lambda 1 levels in chronic hepatitis C: Relation to virus replication and liver injury
Publication date: Available online 26 March 2019Source: Microbial PathogenesisAuthor(s): Hoda El Aggan, Nahla Farahat, Nevine El Deeb, Ahmed Zeid, Assem El-ShendidiAbstractBackground and aimToll-like receptor 7 (TLR7) can recognize single-stranded RNA viruses like hepatitis C virus (HCV) with subsequent induction of different interferon (IFN) types including IFN lambda (IFNL), which activate an immediate anti-viral response. However, the role of TLR7 in inflammation and fibrosis, characteristics of HCV-induced liver injury, is still controversial. The present work was designed to investigate the potential role of TLR7 and ...
Source: Microbial Pathogenesis - March 27, 2019 Category: Infectious Diseases Source Type: research

A review of the current evidence of fruit phenolic compounds as potential antimicrobials against pathogenic bacteria
Publication date: Available online 24 March 2019Source: Microbial PathogenesisAuthor(s): M.C. Lima, C. Paiva de Sousa, C. Fernandez-Prada, J. Harel, J.D. Dubreuil, E.L. de SouzaAbstractFruits are among the main natural sources of phenolic compounds (PC). These compounds exert important antioxidant properties primarily associated with the presence of hydroxyl groups in their molecular structure. Additionally, the antibacterial effects of fruit phenolic-rich extracts or individual PC commonly found in fruits have been an emerging research focus in recent years. This review discusses by first time the available literature reg...
Source: Microbial Pathogenesis - March 25, 2019 Category: Infectious Diseases Source Type: research

Immunologic balance of regulatory T cell/T helper 17 responses in gastrointestinal infectious diseases: Role of miRNAs
In conclusion, there is an imbalance in the Treg/Th17 ratio in GIDs; importantly, sets of miRNAs, particularly miR155 and miR146, were determined to be involved clearly in GIDs. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - March 25, 2019 Category: Infectious Diseases Source Type: research

Computational screening of potential drug targets for pathogens causing bacterial pneumonia
Publication date: Available online 23 March 2019Source: Microbial PathogenesisAuthor(s): Subhalaxmi Nayak, Dibyabhaba Pradhan, Harpreet Singh, M. Sudhakar ReddyAbstractStreptococcus pneumoniae is widely recognized as the main cause of bacterial pneumonia among all age groups. Other important gram-positive, gram-negative and atypical bacteria causing pneumonia majorly infect children and infants. Despite abundant occurrence of bacterial pneumonia, there is no specific antibiotic therapy available. On the other hand non-specific therapies are less effective and may influence bacterial resistance. Therefore, search for novel ...
Source: Microbial Pathogenesis - March 25, 2019 Category: Infectious Diseases Source Type: research