Proton-pump inhibitor and amoxicillin-based triple therapy containing clarithromycin versus metronidazole for Helicobacter pylori: A meta-analysis
Publication date: Available online 16 February 2020Source: Microbial PathogenesisAuthor(s): Bo Li, Xiaoqian Lan, Li Wang, Jiani Zhao, Jingli Ding, Hao Ding, Jun Lei, Yiping Wei, Wenxiong ZhangAbstractBackgroundHelicobacter pylori (H. pylori) infection is one of the important risk factors of gastric related diseases and antibiotic therapy has become an effective treatment. At present, proton-pump inhibitor and amoxicillin-based triple therapy, including clarithromycin (PAC) and metronidazole (PAM), are two commonly used first-line therapies for H. pylori infection, which has a high incidence and possibly poor prognosis worl...
Source: Microbial Pathogenesis - February 17, 2020 Category: Infectious Diseases Source Type: research

Mechanistic study of the interaction between IL-22 and HCV core protein in the development of hepatocellular carcinoma among liver transplant recipients
ConclusionHCV core and IL-22 and SOCS-3 molecules are found to be correlated statistically under this study. Concluded from this study that HCV core protein plays a potential role in diverging the hepatocytes from normal to carcinogenic. One cell signaling path cannot decide, the direct role of a single viral protein in developing viral induced hepatocarcinogenesis. Interpreting the complex network of cell signaling involved in HCC development is impractical to study under single study. That is why step by step unmasking the interactive role of few molecules under single study is the ideal way to resolve the impact of vira...
Source: Microbial Pathogenesis - February 16, 2020 Category: Infectious Diseases Source Type: research

Antibacterial activity profile of miramistin in in vitro and in vivo models
ConclusionsThe results of this work provide systematic data on antibacterial efficacy of miramistin. They also underscore the need in relevant in vivo models for evaluation of antiseptics' efficacy. While the existing in vitro methods can be successfully applied at the discovery stages, it is necessary to use more realistic in vivo models at more advanced development stages. The observed selectivity reversal effect should be taken into account when carrying out the antiseptics’ efficacy testing and surface disinfection procedures. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

Polyketides from marine-derived Aspergillus welwitschiae inhibit Staphylococcus aureus virulence factors and potentiate vancomycin antibacterial activity in vivo
This study reports anti-virulence activity against S. aureus, including a MRSA clinical isolate, of naphto-γ-pyrones from Aspergillus welwitschiae mycelium, and the characterization of chemical constituents by LC-DAD and LC-MS. A remarkable decrease in biofilm formation, hemolysis and coagulation promoted by Staphylococcus aureus, important traits for bacterial pathogenicity, were observed. Furthermore, advantageous association with vancomycin resulted in significant protection against staphylococcal infection in Galleria mellonella model. These findings corroborate the importance of natural products as a source of n...
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

Development of an immunocompetent murine model of pulmonary infection due to Scedosporium apiospermum
Publication date: Available online 15 February 2020Source: Microbial PathogenesisAuthor(s): Carolina E. Luna-Rodríguez, Rogelio J. de Treviño-Rangel, Adolfo Soto-Domínguez, Miguel A. Becerril-García, Martin A. González-Montalvo, Andrea M. Saldivar M, Humberto Rodríguez-Rocha, Gloria M. GonzalezAbstractA pulmonary infection model due to Scedosporium apiospermum in immunocompetent mice was developed. BALB/c mice were infected by endotracheal intubation with 5 × 106 conidia/mouse and disease progression was evaluated on days 1, 3, 5, 7, 11, 16, 21, 30, 50 and 60 post-infecti...
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

Identification of begomoviruses from different cryptic species of Bemisia tabaci in Bangladesh
This study demonstrates the geographic distribution of various begomoviruses in Bangladesh and their relationships with cryptic species of B. tabaci.Graphical abstract (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF−κB activation
ConclusionThese results indicated that CUR is superior to CIP in the prevention of mastitis, and the mechanism may be that the curative effect of CUR inhibits TLR-2 mediated NF-κB signaling pathway in mouse mastitis. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

virB11 gene potentially involves in ATP metabolism to provide energy in H. pylori infection
In this study, the structure and biology of virB11 gene were analyzed and elucidated with bioinformatics analysis. After cloning, expression and purification, VirB11 protein was generated for the cytotoxicity to GES-1 cells and the anti-VirB11 protein antibody production for localization and interaction proteins analysis. The results showed that VirB11 protein is a hydrophilic protein, mainly locates in cell membrane. IL-8 productions from GES-1 cells co-culture with VirB11 protein were increased gradually with time (p 
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

Comparison of immune responses in guinea pigs by intranasal delivery with different nanoparticles-loaded FMDV DNA vaccine
In conclusion, CpG and IFN-α adjuvant based FMD vaccines elicit protection in guinea pigs. Moreover, CS-coated PLGA NPs present an efficient and safe mucosal immune delivery system for FMDV DNA vaccine. Data from the current study provide a foundation for understanding and further evaluating protective immune responses in pigs. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

Role of bacterial Ser/Thr protein kinases spr 1577 in the pathogenesis of respiratory infections caused by S. pneumoniae
This study investigated the specific role played by conserved membrane eukaryotic-like Ser/Thr protein kinases (StkP) during infection, particularly pneumonia, in order to get deep insights about Ser/Thr protein kinases signaling during infection and pathogenesis. The wild type and mutated Ser/Thr protein kinases (Spr1577) strains were tested on different cell lines to understand their virulence based on gentamicin protection assay, gene expression analysis and the expression of various interleukins. Though there was no uptake of bacterial CFU at the beginning, after 24 h it was 50% less for the mutated in comparison to ...
Source: Microbial Pathogenesis - February 15, 2020 Category: Infectious Diseases Source Type: research

Antimicrobial potential of the food-grade additive carvacrol against uropathogenic E. coli based on membrane depolarization, reactive oxygen species generation, and molecular docking analysis
Publication date: Available online 12 February 2020Source: Microbial PathogenesisAuthor(s): Imran Khan, Ashutosh Bahuguna, Shruti Shukla, Faisal Aziz, Anil Kumar Chauhan, Mohd Bismillah Ansari, Vivek K. Bajpai, Yun Suk Huh, Sun Chul KangAbstractThe antibiotic resistance of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased drastically in recent years. In our study, we determined the principle mechanisms of action for the food-grade additive carvacrol against ESBL E. coli isolated from the blood of patients with a urinary tract infection. Carvacrol, which has a minimum inhibitory concentratio...
Source: Microbial Pathogenesis - February 14, 2020 Category: Infectious Diseases Source Type: research

Antimicrobial activity of Lactobacillus fermentum TcUESC01 against Streptococcus mutans UA159
Publication date: Available online 12 February 2020Source: Microbial PathogenesisAuthor(s): Jeisa Zielle de Souza Rodrigues, Manuela Ribeiro Passos, Nayara Silva de Macêdo Neres, Rafael Silva Almeida, Louise Soares Pita, Iago Almeida Santos, Paulo Henrique Santana Silveira, Mariane Mares Reis, Isabella Porto Santos, Luccas de Oliveira Negrão Ricardo, Brenda Oliveira Lima, Patrick D'Orleans Farias Marinho, Ananda Brito Soares, Leonardo Oliveira Silva Bastos Andrade, Stela Mares Brasileiro Pessoa, Marlon Mário Leles Silva, Milena Cardoso Oliveira, Jamile Pinheiro da Silva, Mariana Araújo Moura, Mar...
Source: Microbial Pathogenesis - February 14, 2020 Category: Infectious Diseases Source Type: research

Staphylococcal enterotoxin B increased severity of experimental model of multiple sclerosis
In this study we investigated the role of staphylococcal enterotoxin B in immunologic and pathologic changes in experimental animal model of multiple sclerosis.MethodsC57BL/6 female mice were treatment with SEB protein prior or post immunization with MOG33-35 peptide. Mice were monitored daily and scored for clinical symptoms following EAE induction. Spleen and spinal cord of mice were removed and used for ELISA and histological studies, respectively.ResultsTreatment with SEB prior induction of EAE, increased clinical score, the concentration of IL-17A, IFN-γ and histological changes compared to control group. Treatm...
Source: Microbial Pathogenesis - February 14, 2020 Category: Infectious Diseases Source Type: research

Quorum sensing system: Target to control the spread of bacterial infections
Publication date: Available online 13 February 2020Source: Microbial PathogenesisAuthor(s): Erika Kushikawa Saeki, Renata Katsuko Takayama Kobayashi, Gerson NakazatoAbstractQuorum Sensing (QS) systems regulate the gene expression of different types of virulence factors in accordance with the cell population density. A literature search was performed, including electronic databases such as MEDLINE/PubMed, SciELO, and LILACS, as well as other databases not indexed, such as Google Scholar. The search was conducted between July 2018 and April 2019, through online research. Antimicrobial resistance is one of the biggest threats...
Source: Microbial Pathogenesis - February 14, 2020 Category: Infectious Diseases Source Type: research

The distribution characteristics of intestinal microbiota in children with community-acquired pneumonia under 5 Years of age
Publication date: Available online 11 February 2020Source: Microbial PathogenesisAuthor(s): Xiaomeng Ren, Yaser Gamallat, Dongjie Liu, Yanyan Zhu, Abdo Meyiah, Sadia Kanwal, Dong Shang, Yi XinAbstractPneumonia is the leading cause of morbidity and mortality in children under 5 years of age worldwide. Over the past decades, studies have shown that the upper respiratory pathogens are closely related to the occurrence of pneumonia. However, the co-occurrence of gut microbiome dysbiosis may have clinical manifestation in the prognosis of childhood pneumonia. The scope of the present study is to investigate the differences in g...
Source: Microbial Pathogenesis - February 12, 2020 Category: Infectious Diseases Source Type: research

Effect of flagellin on inhibition of infectious mechanisms by activating opsonization and salmonella flagellum disruption
Publication date: Available online 11 February 2020Source: Microbial PathogenesisAuthor(s): Mohammad-Hosein Khani, Masoumeh Bagheri, Azadeh Zahmatkesh, Khosrow Aghaiypour, Ali MirjaliliAbstractSome serovars of salmonella cause huge global diseases such as enteric fever and invasive non typhoidal Salmonella disease. Flagellin as a key antigenic component of salmonella, can induce humoral and cellular immunity responses. In this research, we performed an opsonophagocytic killing assay (OPKA) as an important mechanism of the host-defense system, for salmonella to study the activity of anti-sera of native FliC, truncated modif...
Source: Microbial Pathogenesis - February 12, 2020 Category: Infectious Diseases Source Type: research

Antimicrobial mechanism of luteolin against Staphylococcus aureus and Listeria monocytogenes and its antibiofilm properties
This study aimed to investigate the antimicrobial activities of LUT and its mode of action against Staphylococcus aureus and Listeria monocytogenes, either as planktonic cells or as biofilms. Here, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of LUT against S. aureus and L. monocytogenes were determined using the broth microdilution method, and the antimicrobial mode of LUT was elucidated by evaluating the variations in both cell membrane integrity and cell morphology. Moreover, the biofilm inhibition was measured by crystal violet staining assay, while its qualitative imaging was ac...
Source: Microbial Pathogenesis - February 12, 2020 Category: Infectious Diseases Source Type: research

Changes in intestinal microbiota and disease resistance following dietary postbiotic supplementation in rainbow trout (Oncorhynchus mykiss)
Publication date: Available online 11 February 2020Source: Microbial PathogenesisAuthor(s): Tania Pérez-Sánchez, Brenda Mora-Sánchez, Augusto Vargas, José Luis BalcázarAbstractThis experimental study was aimed to investigate whether the dietary supplementation of a postbiotic obtained as a food product fermented with two lactic acid bacteria could induce changes in the intestinal microbiota and prevent the development of Lactococcus garvieae infection in rainbow trout (Oncorhynchus mykiss). After 30 days of dietary postbiotic supplementation, bacterial community composition and structure ...
Source: Microbial Pathogenesis - February 12, 2020 Category: Infectious Diseases Source Type: research

Schizandrin attenuates lung lesions induced by Avian pathogenic Escherichia coli in chickens
In conclusion, our findings reveal that schizandrin displays anti-oxidant and anti-inflammatory activity against APEC-induced lung lesions in chickens, paving the way for rational use of schizandrin as a protective agent against lung-related inflammatory disease. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 12, 2020 Category: Infectious Diseases Source Type: research

Identification of pmrB mutations as putative mechanism for colistin resistance in A. baumannii strains isolated after in vivo colistin exposure
Publication date: Available online 11 February 2020Source: Microbial PathogenesisAuthor(s): Vito Marano, Nadia Marascio, Grazia Pavia, Angelo G. Lamberti, Angela Quirino, Rosanna Musarella, Francesco Casalinuovo, Maria Mazzitelli, Enrico M. Trecarichi, Carlo Torti, Giovanni Matera, Maria Carla LibertoAbstractColistin resistance among extensively-resistant Acinetobacter baumannii isolates is a serious health-care problem. Alterations in PmrA-PmrB two-component system have been associated with resistance to colistin. We investigated three pairs of colistin-susceptible and colistin-resistant A. baumannii, sequentially isolate...
Source: Microbial Pathogenesis - February 12, 2020 Category: Infectious Diseases Source Type: research

Characterization and pathogenicity of extracellular serine protease MAP3292c from Mycobacterium avium subsp. paratuberculosis
Publication date: Available online 11 February 2020Source: Microbial PathogenesisAuthor(s): Hongxiu Liu, Guanghui Dang, Xinxin Zang, Zhuming Cai, Ziyin Cui, Ningning Song, Siguo LiuAbstractSerine protease is the virulence factor of many pathogens. However, there are no prevailing data available for serine protease as a virulence factor derived from Mycobacterium avium subsp. paratuberculosis (MAP). The MAP3292c gene from MAP, the predicted serine protease, was expressed in Escherichia coli and characterized by biochemical methods. MAP3292c protein efficiently hydrolyzed casein at optimal temperature and pH of 41 °C a...
Source: Microbial Pathogenesis - February 12, 2020 Category: Infectious Diseases Source Type: research

Genomic diversity in flavobacterial pathogens of aquatic origin
In conclusion, comparative genomics of Flavobacterium species of aquatic origin will help our understanding of Flavobacterium pathogenesis. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 11, 2020 Category: Infectious Diseases Source Type: research

A potential antifungal and growth-promoting bacterium Bacillus sp. KTMA4 from tomato rhizosphere
Publication date: Available online 8 February 2020Source: Microbial PathogenesisAuthor(s): S. Karthika, Sebastian Jose Midhun, M.S. JishaAbstractPlant growth-promoting rhizobacteria are indigenous beneficial bacteria that will enhance plant growth as well as suppress phytopathogens. In the present study, the isolate KTMA4 showed the highest inhibition against major phytopathogens of tomato; Fusarium oxysporum (66%) and Alternaria solani (54%) after seven days of incubation. Analysis of the 16S rRNA gene sequence revealed that the isolate KTMA4 is Bacillus cereus (MG547975). The isolate produced in vitro plants growth-promo...
Source: Microbial Pathogenesis - February 9, 2020 Category: Infectious Diseases Source Type: research

Combinations of early generation antibiotics and antimicrobial peptides are effective against a broad spectrum of bacterial biothreat agents
In this study, the therapeutic potential of combinations of an antibiotic and a broad-spectrum antimicrobial peptide (AMP) was evaluated against five bacterial biothreats, the etiologic agents of glanders (Burkholderia mallei), melioidosis (Burkholderia pseudomallei), plague (Yersinia pestis), tularemia (Francisella tularensis), and anthrax (Bacillus anthracis). The therapeutics included licensed early generation antibiotics which are now rarely used. Three antibiotics and one 24- amino acid AMP were selected based on MIC assay data. Combinations of the AMP and tigecycline, minocycline, or novobiocin were screened for syne...
Source: Microbial Pathogenesis - February 9, 2020 Category: Infectious Diseases Source Type: research

Neisseria gonorrhoeae-induced salpingitis is targeted by circular RNA EIF3K via miR-139-5p and regulating MAPK/NF-κB signaling pathway to promotes apoptosis and autophagy bacterial cells
ConclusionCircular EIF3K suppressed cell growth in salpingitis through inhibiting MAPK/NK-κB signaling pathway, which could be a potential factor for further studies in slpingitis. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 8, 2020 Category: Infectious Diseases Source Type: research

Bacterial biofilm in colorectal cancer: What is the real mechanism of action?
Publication date: Available online 8 February 2020Source: Microbial PathogenesisAuthor(s): Rasoul Mirzaei, Hamed Mirzaei, Mohammad Yousef Alikhani, Mohammad Sholeh, Mohammad Reza Arabestani, Masoud Saeedijam, Sajad Karampoor, Yaghoub Ahmadyousefi, Mohammad Shokri Moghadam, Gholam Reza Irajian, Hamze Hasanvand, Rasoul YousefimashoufAbstractHuman colorectal cancer is the third most common cancer around the world. Colorectal cancer has various risk factors, but current works have bolded a significant activity for the microbiota of the human colon in the development of this disease. Bacterial biofilm has been mediated to non-m...
Source: Microbial Pathogenesis - February 8, 2020 Category: Infectious Diseases Source Type: research

Antimicrobial resistance of three common molecularly identified pathogenic bacteria to Allium aqueous extracts
Publication date: Available online 8 February 2020Source: Microbial PathogenesisAuthor(s): Daniel A. Serrano, María A. Mariezcurrena-Berasain, Adriana Del Carmen Gutiérrez Castillo, Benjamín Valladares Carranza, Alberto Barbabosa Pliego, Martín Talavera Rojas, Uchenna Y. Anele, Abdelfattah Z-M. Salem, Raymundo R. Rivas-CaceresAbstractThe aim of this work was to evaluate the in vitro bacterial inhibition of different types of garlic on Escherichia coli ATCC 25922, Listeria monocytogenes and Staphylococcus aureus. The bacterial strains were molecularly identified using gen 16S rDNA molecular ident...
Source: Microbial Pathogenesis - February 8, 2020 Category: Infectious Diseases Source Type: research

Microbiological profile of distinct virulence of Nocardia cyriacigeorgica strains in vivo and in vitro
In this study, we explored the differences in virulence and pathogenic mechanisms of two Nocardia cyriacigeorgica strains. We examined the difference in virulence between N. cyriacigeorgica ATCC14759 and N. cyriacigeorgica GUH-2 by measuring cytotoxicity, animal survival after infection, the ability of host cell invasion, and viability in host cells. Western blotting was used to compare the differences in activation of MAPKs, including p38, ERK, and JNK, the NF-κB signaling pathway, and the PI3K/Akt signaling pathway in A549 and RAW264.7 cells. We measured the difference in stimulatory effects on production of the ...
Source: Microbial Pathogenesis - February 8, 2020 Category: Infectious Diseases Source Type: research

Co-infection status of porcine circoviruses (PCV2 and PCV3) and porcine epidemic diarrhea virus (PEDV) in pigs with watery diarrhea in Henan province, central China
Publication date: Available online 6 February 2020Source: Microbial PathogenesisAuthor(s): Zhenhua Guo, Haiyu Ruan, Songlin Qiao, Ruiguang Deng, Gaiping ZhangAbstractPorcine circoviruses (PCV2 and PCV3) and porcine epidemic diarrhea virus (PEDV) are important swine viruses that threaten the swine industry worldwide. Here, we evaluated the co-infection status of PCV2, PCV3 and PEDV in 76 enteric samples from piglets with severe diarrhea disease in Henan, China. All samples were tested by PCR/RT-PCR. Our results showed that the infection rate of PCV2, PCV3 and PEDV was 82.89%, 76.32% and 68.42%, respectively. Interestingly, ...
Source: Microbial Pathogenesis - February 8, 2020 Category: Infectious Diseases Source Type: research

Therapeutic applications of lytic phages in human medicine
Publication date: Available online 5 February 2020Source: Microbial PathogenesisAuthor(s): Zhimin Guo, Hua Lin, Xufeng Ji, Guangmou Yan, Liancheng Lei, Wenyu Han, Jingmin Gu, Jing HuangAbstractThe emergence and spread of antibiotic-resistant bacteria constitute a critical issue for modern medicine. Patients with antibiotic-resistant bacterial infections consume more healthcare resources and have worse clinical outcomes than patients with antibiotic-sensitive bacterial infections. Phages are natural predators of bacteria and may therefore be a source of useful antibacterial drugs. Phage therapy possess availability for oral...
Source: Microbial Pathogenesis - February 6, 2020 Category: Infectious Diseases Source Type: research

Development of vaccines for prevention of peste-des-petits-ruminants virus infection
Publication date: Available online 6 February 2020Source: Microbial PathogenesisAuthor(s): Xue-Xia Jia, Wang Hui, Ying Liu, De-Mei Meng, Zhen-Chuan FanAbstractPeste des petits ruminants (PPR) is a highly contagious and fatal disease of small ruminants, particularly sheep and goats. This disease leads to high morbidity and mortality of small ruminants, thus resulting in devastating economic loss to the livestock industry globally. The severe disease impact has prompted the Food and Agriculture Organization of the United Nations (FAO) and the World Organization for Animal Health (OIE) to develop a global strategy for the con...
Source: Microbial Pathogenesis - February 6, 2020 Category: Infectious Diseases Source Type: research

Cloning and expression of three heat shock protein genes in the gills of Cherax quadricarinatus responding to bacterial challenge
In this study, CqHsp60, CqHsp70 and CqHsp90 from C. quadricarinatus were cloned, and their expression patterns were analysed. The CDS (coding sequence) lengths of the CqHsp60, CqHsp70 and CqHsp90 genes were 1731 bp, 1932 bp and 2199 bp, encoding 576, 643 and 732 amino acids, respectively. CqHsp60 was 99.13%, 98.78% and 88.63% identical to the corresponding sequences of Cherax cainii, Cherax destructor and Eriocheir sinensis, respectively. CqHsp70 showed 99.84%, 92.73% and 91.58% identity to the corresponding sequences of C. cainii, C. destructor and E. sinensis, while CqHsp90 was 98.25%, 98.51% and 91.41% identical with th...
Source: Microbial Pathogenesis - February 5, 2020 Category: Infectious Diseases Source Type: research

A novel cis antisense RNA AsfD promotes Salmonella enterica serovar Typhi motility and biofilm formation
In this study, a northern blot and qRT-PCR were used to confirm the expression of this newfound antisense RNA in S. Typhi. Moreover, 5′ RACE and 3′ RT-PCR were performed to reveal the molecular characteristics of the antisense RNA, which was 2079 nt − 2179 nt in length, covered the entire flhDC operon sequence, and termed AsfD. The level of AsfD expression was higher during the stationary phase of S. Typhi and activated by the regulators, OmpR and Fis. When AsfD was overexpressed, the level of flagellar gene flhDC transcription increased; moreover, the level of fliA and fljB expression, as well as the...
Source: Microbial Pathogenesis - February 5, 2020 Category: Infectious Diseases Source Type: research

Induction of Th2 response through TLR2-mediated MyD88-dependent pathway in human microfold cells stimulated with chitosan nanoparticles loaded with Brucella abortus Mdh
This study will contribute to the development of not only effective antigens for intracellular bacteria, including B. abortus, but also vaccine delivery systems that target M cells. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 5, 2020 Category: Infectious Diseases Source Type: research

Short communication: Uterine leptospiral infection is strongly associated to strains of serogroup Sejroe on experimentally infected hamsters
Publication date: Available online 3 February 2020Source: Microbial PathogenesisAuthor(s): P.S. Pinto, C. Barbosa, A.M.R. Ferreira, W. LilenbaumAbstractLeptospira infection is influenced by the host species and the bacterial strain involved. Important differences on their tissue distribution are referred, also depending on the host and the studied strain. Considering tissue distribution of leptospires the majority of the studies focus on a single strain, or strains from the same serogroup with different inoculation doses. Nevertheless, none had associated different serogroups with their tissue distribution. Thus, the prese...
Source: Microbial Pathogenesis - February 5, 2020 Category: Infectious Diseases Source Type: research

Degeneration of the intestinal microbial community in PI3Kγ-knockout mice
ConclusionsPI3Kγ dysfunction led to degeneration of the intestinal microbial community and might alter the synthesis of some antibiotics, bile acids, and thiamine. The usage of PI3Kγ inhibitors for inflammation and cardiovascular diseases might lead to knock-on effect on our organism through intestinal microbiota. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 5, 2020 Category: Infectious Diseases Source Type: research

SspH2 as anti-inflammatory candidate effector and its contribution in Salmonella Enteritidis virulence
Publication date: Available online 4 February 2020Source: Microbial PathogenesisAuthor(s): Makarem O.E. Shappo, Qiuchun Li, Zhijie Lin, Maozhi Hu, Jingwei Ren, Zhengzhong Xu, Zhiming Pan, Xinan JiaoAbstractSalmonella enterica is a facultative intracellular pathogen deploying the type III secretion system (T3SS) encoded by Salmonella Pathogenicity Island 2 (SPI2) to transfer effector proteins into host cells to modify its functions and accomplish intracellular replication. To study the effect of SspH2 on immune response induced by S. Enteritidis, we generated a deletion mutant of the effector gene sspH2 and a plasmid mediat...
Source: Microbial Pathogenesis - February 5, 2020 Category: Infectious Diseases Source Type: research

Antidermatophytic lead compounds from Streptomycetes albidoflavus STV1572a against Tinea infections by Tricophyton mentagrophytes
Publication date: Available online 3 February 2020Source: Microbial PathogenesisAuthor(s): Aswani Thekkangil, T.V. SuchithraAbstractDermatophytic infection is a psychological burden due to its inefficacy in prolonged effectiveness during its course of treatment. The present study reports a novel antidermatophytic property from actinomycetes of wetlands soil. Total of 37 Streptomyces isolates were screened for antidermatophytic activity against Trichophyton mentagrophytes. The best isolate with 100% novel activity, was characterized and designated as Streptomyces albidoflavus STV1572a. Antidermatophytic compounds extracted ...
Source: Microbial Pathogenesis - February 3, 2020 Category: Infectious Diseases Source Type: research

Smut fungal strategies for the successful infection
Publication date: Available online 3 February 2020Source: Microbial PathogenesisAuthor(s): Wenqiang Xia, Xiaoping Yu, Zihong YeAbstractThe smut fungi include a large number of plant pathogens that establish obligate biotrophic relationships with their host. Throughout the whole life inside plant tissue, smut fungi keep plant cells alive and acquire nutrients via biotrophic interfaces. This mini-review mainly summarizes the interactions between smut fungi and their host plants during the infection process. Despite various strategies recruited by plants to defense invading pathogens, smut fungi successfully evolved an arsena...
Source: Microbial Pathogenesis - February 3, 2020 Category: Infectious Diseases Source Type: research

Insights into penicillin-induced Chlamydia trachomatis persistence
In conclusion, we added knowledge to the cellular dynamics taking place during chlamydial persistence, demonstrating that CT creates a suitable niche to survive, switching off signals able to activate phagocytes/leukocytes recruitment. Nevertheless, persistent CT elicits ROS production by the infected cells, potentially contributing to the onset of chronic inflammation and tissue damages. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 2, 2020 Category: Infectious Diseases Source Type: research

Mechanistic insights into the action of probiotics against bacterial vaginosis and its mediated preterm birth: An overview
Publication date: Available online 31 January 2020Source: Microbial PathogenesisAuthor(s): H.N. Basavaprabhu, K.S. Sonu, R. PrabhaAbstractThe human body is a reservoir of numerous micro creatures; whose role is substantial and indispensable in the overall development of human beings. The advances in omic approaches have offered powerful means to decipher the core microbiome and metabolome diversities in a specific organ system. The establishment of lactobacilli in the female reproductive tract is thought to be a paramount prerequisite that maintains homeostatic conditions for a sustainable and healthy pregnancy. Neverthele...
Source: Microbial Pathogenesis - February 1, 2020 Category: Infectious Diseases Source Type: research

Longitudinal study of BKV outcomes, risk factors, and kinetics in renal transplantation patients
ConclusionsWe identified diabetes and hyperlipidemia as potential risk factors for development of high BK viremia and/or BKVN. IVIG was seen to be effective in reducing viral titers. The period 3–9 months post-transplantation was identified as important for development of BKVN from high BK viremia. (Source: Microbial Pathogenesis)
Source: Microbial Pathogenesis - February 1, 2020 Category: Infectious Diseases Source Type: research

Full-genome sequences of porcine circovirus 3 (PCV3) and high prevalence in mummified fetuses from commercial farms in Brazil
Publication date: Available online 30 January 2020Source: Microbial PathogenesisAuthor(s): Ana Carolina Dal Santo, Kamila C. Cezario, Paulo E. Bennemann, Sergio A. Machado, Mathias MartinsAbstractThe clinical implications of recently discovered porcine circovirus 3 (PCV3) infections are still unknown. The potential role of this emerging virus in reproductive loss in swine has been described. Herein, we report a high prevalence of PCV3 in mummified fetuses from sows maintained in modern farms in Rio Grande do Sul, Santa Catarina, Paraná, Goiás, and Mato Grosso do Sul states, Brazil. For this analysis, 276 mumm...
Source: Microbial Pathogenesis - January 31, 2020 Category: Infectious Diseases Source Type: research

Modulation of drug resistance in Staphylococcus aureus by 132-hydroxy-(132-R/S)-pheophytin isolated from Sargassum polyceratium
Publication date: Available online 30 January 2020Source: Microbial PathogenesisAuthor(s): Suellen Menezes-Silva, Narlize Silva Lira, Yuri Mangueira do Nascimento, Roseana Ramos, Celidarque da Silva Dias, Josean Fechine Tavares, Marcelo Sobral da Silva, George Emmanuel Cavalcanti de Miranda, José Maria Barbosa Filho, José Pinto de Siqueira-JuniorAbstractEfflux pumps are integral parts of the bacterial plasma membrane that are responsible for many cases of antibiotic resistance. Modulators of drug resistance are regarded as the most suitable new antibacterial therapies. We evaluated the extracts of Sargassum p...
Source: Microbial Pathogenesis - January 31, 2020 Category: Infectious Diseases Source Type: research

The oncogenic roles of bacterial infections in development of cancer
Publication date: Available online 30 January 2020Source: Microbial PathogenesisAuthor(s): Shirin Eyvazi, Mehdi Asghari Vostakolaei, Azita Dilmaghani, Omid Borumandi, Mohammad Saeid Hejazi, Houman Kahroba, Vahideh TarhrizAbstractInitiation of cancer is interconnected with different factors like infections. It has been estimated that infections, particularly viruses, participate in about 20% of all cancers. Bacteria as the most common infectious agents are also reported to be emerging players in the establishment of malignant cells. Microbial infections are able to modulate host cell transformation for promoting malignant f...
Source: Microbial Pathogenesis - January 30, 2020 Category: Infectious Diseases Source Type: research

Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages
Publication date: Available online 30 January 2020Source: Microbial PathogenesisAuthor(s): Sofía C. Sacco, Natalia S. Velázquez, María S. Renna, Camila Beccaria, Celina Baravalle, Elizabet A.L. Pereyra, Stefan Monecke, Luis F. Calvinho, Bibiana E. DallardAbstractThe aim of this study was to evaluate and compare the ability to adhere/internalize, persist, and induce damage in mammary epithelial cells (MAC-T) of two Staphylococcus aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG). Also, the phagocytic and bactericidal capacity induced after the interaction...
Source: Microbial Pathogenesis - January 30, 2020 Category: Infectious Diseases Source Type: research

Influence of apitoxin and melittin from Apis mellifera bee on Staphylococcus aureus strains
Publication date: Available online 28 January 2020Source: Microbial PathogenesisAuthor(s): Ana Flávia Marques Pereira, Mariana Albano, Fernanda Cristina Bérgamo Alves, Bruna Fernanda Murbach Teles Andrade, Alessandra Furlanetto, Vera Lúcia Mores Rall, Lucilene Delazari dos Santos, Ricardo de Oliveira Orsi, Ary Fernandes JúniorAbstractThe antibacterial activities of apitoxin, a venom produced by Apis mellifera bee, and melittin, an antimicrobial peptide from apitoxin, were tested against planktonic and biofilm states of Staphylococcus aureus methicillin-resistant (MRSA), including clinical, and e...
Source: Microbial Pathogenesis - January 30, 2020 Category: Infectious Diseases Source Type: research

Global estimate of phenotypic and genotypic ganciclovir resistance in cytomegalovirus infections among HIV and organ transplant patients; A systematic review and meta-analysis
Publication date: Available online 29 January 2020Source: Microbial PathogenesisAuthor(s): Taher Azimi, Shaian Tavakolian, Hossein Goudarzi, Mohammad Reza Pourmand, Ebrahim FaghihlooAbstractHuman cytomegalovirus (CMV), an opportunistic pathogen belonging to Herpesviridae family, is considered as one of the major causes of morbidity and mortality among wide variety of patients, particularly in transplant recipients and HIV positive patients. As this virus can be resistant to treatment, frequency of CMV in patients who receive organ transplantation and people suffering from AIDS was studied between 1980 and 2019. Medline (vi...
Source: Microbial Pathogenesis - January 30, 2020 Category: Infectious Diseases Source Type: research

Aspergillus coinfection among patients with pulmonary tuberculosis in Asia and Africa countries; A systematic review and meta-analysis of cross-sectional studies
This study aimed to determine the prevalence of pulmonary Aspergillus coinfection among patients with pulmonary tuberculosis in Asia and Africa.The present review of cross-sectional studies was conducted on the prevalence of pulmonary Aspergillus coinfection among patients with pulmonary tuberculosis according to the PRISMA Protocol. Literatures published online in English from January 2001 to March 2019 via key databases such as Web of Science, MEDLINE, PubMed, Scopus, and Cochrane Library were searched. The used MeSH and non-MeSH keywords were; “pulmonary fungal”, “pulmonary coinfection”, OR &ldqu...
Source: Microbial Pathogenesis - January 30, 2020 Category: Infectious Diseases Source Type: research

Contribution of endotoxin to Th17 bias in patients with non-alcoholic steatohepatitis
In this study, we investigated the effect of serum endotoxin on CD4 T cell inflammation. Age and sex-matched non-obese healthy subjects, subjects with non-alcoholic fatty liver (NAFL) but not steatohepatitis, and NASH patients were recruited for this study. The latter two groups were additionally matched in BMI and diabetes status. We first showed that compared to healthy subjects and NAFL patients, NASH patients presented significantly higher levels of serum endotoxin. Concurrently, NASH patients presented a Th17 bias that was associated with high endotoxin levels. To examine whether endotoxin could directly mediate IL-17...
Source: Microbial Pathogenesis - January 28, 2020 Category: Infectious Diseases Source Type: research