Impact of template denaturation prior to whole genome amplification on gene detection in high GC-content species, Burkholderia mallei and B. pseudomallei
In this study, we sought to determine the types and prevalence of antimicrobial resistance determinants (ARDs) in Burkholderia spp. strains using the Antimicrobial Resistance Determinant Microarray (ARDM). (Source: BMC Research Notes)
Source: BMC Research Notes - March 12, 2024 Category: Research Authors: Chris R. Taitt, Tomasz A. Leski, Jaimee R. Compton, Amy Chen, Kimberly L. Berk, Robert W. Dorsey, Shanmuga Sozhamannan, Dianne L. Dutt and Gary J. Vora Tags: Research Note Source Type: research
Spread analysis of glanders in the state of Piau í, northeastern Brazil
Comp Immunol Microbiol Infect Dis. 2024 Feb;105:102122. doi: 10.1016/j.cimid.2023.102122. Epub 2024 Jan 2.ABSTRACTBrazil is strategic in controlling neglected zoonoses, such as glanders, in its territory. Among the Brazilian states, Piauí is a strategic state for the spread of the disease in the country. The present study aimed to evaluate the spatial and temporal distribution of official cases of glanders in Piauí between 2015 and 2022. The glanders cases were located in the municipalities of the north and central-north mesoregions, mainly in Campo Maior, Teresina and Altos. The highest incidence risk (IR) occurred in o...
Source: Comparative immunology, microbiology and infectious diseases. - January 14, 2024 Category: Allergy & Immunology Authors: Roselma de Carvalho Moura Francisco Alyson de Oliveira Dayseanny de Oliveira Bezerra Raizza Eveline Esc órcio Pinheiro Ricardo Ant ônio Pilegi Sfaciotte Joandes Henrique Fonteque Sandra Maria Ferraz Raylson Pereira de Oliveira Ver ônica Flores da Cunha Source Type: research
Foremost report of the whole genome of Spirabiliibacterium mucosae from India and comparative genomics of the novel genus Spirabiliibacterium
Gene. 2023 Mar 12:147359. doi: 10.1016/j.gene.2023.147359. Online ahead of print.ABSTRACTSeveral Pasteurella like organisms isolated from various avian species were recently reclassified into new genus based on whole genome sequence analysis. One such Pasteurella like organism, Bisgaard taxon 14 was classified as Spirabiliibacterium mucosae. In the present study, a Gram-negative organism was isolated from ailing pigeons with respiratory infection from a farm in Tamil Nadu, India and the organism was misidentified as Burkholderia mallei by Vitek 2 compact system based on biochemical characterization. Since, B. mallei is hig...
Source: Gene - March 14, 2023 Category: Genetics & Stem Cells Authors: Kumaragurubaran Karthik Subbaiyan Anbazhagan Murugesan Ananda Chitra Rajendran Ramya Ramaswamy Sridhar Gopal Dhinakar Raj Source Type: research
Foremost report of the whole genome of Spirabiliibacterium mucosae from India and comparative genomics of the novel genus Spirabiliibacterium
Gene. 2023 Mar 12:147359. doi: 10.1016/j.gene.2023.147359. Online ahead of print.ABSTRACTSeveral Pasteurella like organisms isolated from various avian species were recently reclassified into new genus based on whole genome sequence analysis. One such Pasteurella like organism, Bisgaard taxon 14 was classified as Spirabiliibacterium mucosae. In the present study, a Gram-negative organism was isolated from ailing pigeons with respiratory infection from a farm in Tamil Nadu, India and the organism was misidentified as Burkholderia mallei by Vitek 2 compact system based on biochemical characterization. Since, B. mallei is hig...
Source: Gene - March 14, 2023 Category: Genetics & Stem Cells Authors: Kumaragurubaran Karthik Subbaiyan Anbazhagan Murugesan Ananda Chitra Rajendran Ramya Ramaswamy Sridhar Gopal Dhinakar Raj Source Type: research
Foremost report of the whole genome of Spirabiliibacterium mucosae from India and comparative genomics of the novel genus Spirabiliibacterium
Gene. 2023 Mar 12:147359. doi: 10.1016/j.gene.2023.147359. Online ahead of print.ABSTRACTSeveral Pasteurella like organisms isolated from various avian species were recently reclassified into new genus based on whole genome sequence analysis. One such Pasteurella like organism, Bisgaard taxon 14 was classified as Spirabiliibacterium mucosae. In the present study, a Gram-negative organism was isolated from ailing pigeons with respiratory infection from a farm in Tamil Nadu, India and the organism was misidentified as Burkholderia mallei by Vitek 2 compact system based on biochemical characterization. Since, B. mallei is hig...
Source: Gene - March 14, 2023 Category: Genetics & Stem Cells Authors: Kumaragurubaran Karthik Subbaiyan Anbazhagan Murugesan Ananda Chitra Rajendran Ramya Ramaswamy Sridhar Gopal Dhinakar Raj Source Type: research
Expression of virulence and antimicrobial related proteins in < i > Burkholderia mallei < /i > and < i > Burkholderia pseudomallei < /i >
ConclusionsAnalyzing the expressed proteomes revealed differences betweenB.mallei andB.pseudomallei but also between isolates from the same species. Proteome analysis can be used not only to identifyB.mallei andB.pseudomallei but also to characterize the presence of important factors that putatively contribute to the pathogenesis ofB.mallei andB.pseudomallei. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - January 6, 2023 Category: Tropical Medicine Authors: Armand Paauw Source Type: research
Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics
Journal of Clinical Microbiology, Ahead of Print. (Source: Journal of Clinical Microbiology)
Source: Journal of Clinical Microbiology - December 21, 2022 Category: Microbiology Authors: Gabriel E. Wagner,
Andreas Berner,
Michaela Lipp,
Christian Kohler,
Karoline Assig,
Sabine Lichtenegger,
Muhammad Saqib,
Elke M üller,
Trung T. Trinh,
Anne-Marie Gad,
Hans Hermann Söffing,
Ralf Ehricht,
Karine Laroucau,
Ivo Steinmetz
aDiagn Source Type: research
Evaluation of two different vaccine platforms for immunization against melioidosis and glanders
Burkholderia pseudomallei and the closely related species, Burkholderia mallei, produce similar multifaceted diseases which range from rapidly fatal to protracted and chronic, and are a major cause of mortality in endemic regions. Besides causing natural infections, both microbes are Tier 1 potential biothreat agents. Antibiotic treatment is prolonged with variable results, hence effective vaccines are urgently needed. The purpose of our studies was to compare candidate vaccines that target both melioidosis and glanders to identify the most efficacious one(s) and define residual requirements for their transition to the non...
Source: Frontiers in Microbiology - August 17, 2022 Category: Microbiology Source Type: research
A case report of Burkholderia mallei infection leading to pneumonia
CONCLUSIONS: Glanders is a rare disease that can cause skin infection, lymphadenitis, and pneumonia and in severe cases, it can be life-threatening. The diagnosis of this disease mainly relies on microbiological culture and pathological biopsy. Diagnosis is also facilitated by multiplex PCR-based targeted gene sequencing facilitates the diagnosis of this disease. Glanders is treated with cephalosporins, carbapenems, and other sensitive antibiotics.PMID:35579163 | DOI:10.2174/1386207325666220509152221 (Source: Combinatorial Chemistry and High Throughput Screening)
Source: Combinatorial Chemistry and High Throughput Screening - May 17, 2022 Category: Chemistry Authors: Guanfeng He Yu Zeng Qizhong He Tuxuan Liu Nanhong Li Hui Lin Muhong Zeng Yonglong Li Min Peng Junfen Cheng Wang Liu Weimin Yao Source Type: research
A case report of Burkholderia mallei infection leading to pneumonia
CONCLUSIONS: Glanders is a rare disease that can cause skin infection, lymphadenitis, and pneumonia and in severe cases, it can be life-threatening. The diagnosis of this disease mainly relies on microbiological culture and pathological biopsy. Diagnosis is also facilitated by multiplex PCR-based targeted gene sequencing facilitates the diagnosis of this disease. Glanders is treated with cephalosporins, carbapenems, and other sensitive antibiotics.PMID:35579163 | DOI:10.2174/1386207325666220509152221 (Source: Combinatorial Chemistry and High Throughput Screening)
Source: Combinatorial Chemistry and High Throughput Screening - May 17, 2022 Category: Chemistry Authors: Guanfeng He Yu Zeng Qizhong He Tuxuan Liu Nanhong Li Hui Lin Muhong Zeng Yonglong Li Min Peng Junfen Cheng Wang Liu Weimin Yao Source Type: research
A case report of Burkholderia mallei infection leading to pneumonia
CONCLUSIONS: Glanders is a rare disease that can cause skin infection, lymphadenitis, and pneumonia and in severe cases, it can be life-threatening. The diagnosis of this disease mainly relies on microbiological culture and pathological biopsy. Diagnosis is also facilitated by multiplex PCR-based targeted gene sequencing facilitates the diagnosis of this disease. Glanders is treated with cephalosporins, carbapenems, and other sensitive antibiotics.PMID:35579163 | DOI:10.2174/1386207325666220509152221 (Source: Combinatorial Chemistry and High Throughput Screening)
Source: Combinatorial Chemistry and High Throughput Screening - May 17, 2022 Category: Chemistry Authors: Guanfeng He Yu Zeng Qizhong He Tuxuan Liu Nanhong Li Hui Lin Muhong Zeng Yonglong Li Min Peng Junfen Cheng Wang Liu Weimin Yao Source Type: research
First glanders cases detected in Nepal underscore the need for surveillance and border controls
Glanders is a transmissible zoonotic disease caused by Burkholderia mallei that infects equids and humans. No glanders cases in equids were reported so far in Nepal. (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - April 6, 2022 Category: Veterinary Research Authors: Koirala P, Maharjan M, Manandhar S, Pandey KR, Deshayes T, Wang G, Valvano MA and Laroucau K Tags: Case Report Source Type: research
Glanders and brucellosis in equids from the Amazon region, Brazil
Acta Trop. 2022 Mar 25:106429. doi: 10.1016/j.actatropica.2022.106429. Online ahead of print.ABSTRACTGlanders and brucellosis are zoonotic infectious diseases that affect equids in several countries worldwide. On Marajó Island (Amazon region of Brazil), Marajoara and Puruca horses, which are well adapted to the climatic and territorial adversities of the region, play a fundamental role in the local economy and in the sociocultural lives of the population. However, these animals have undergone a drastic reduction in number, markedly due to precarious veterinary care, unknown causes of morbidity and mortality, and disordere...
Source: Acta Tropica - March 29, 2022 Category: Infectious Diseases Authors: Cl áudia Fideles Resende Alison Miranda Dos Santos Paulo Martins Soares Filho Patr ícia Gomes de Souza Marina de Azevedo Issa Maur ício Baltazar de Carvalho Filho Raphael Mattoso Victor Rebeca J éssica Falcão Câmara Gilberto Pereira Gon çalves Juli Source Type: research
Glanders and brucellosis in equids from the Amazon region, Brazil
Acta Trop. 2022 Mar 25:106429. doi: 10.1016/j.actatropica.2022.106429. Online ahead of print.ABSTRACTGlanders and brucellosis are zoonotic infectious diseases that affect equids in several countries worldwide. On Marajó Island (Amazon region of Brazil), Marajoara and Puruca horses, which are well adapted to the climatic and territorial adversities of the region, play a fundamental role in the local economy and in the sociocultural lives of the population. However, these animals have undergone a drastic reduction in number, markedly due to precarious veterinary care, unknown causes of morbidity and mortality, and disordere...
Source: Acta Tropica - March 29, 2022 Category: Infectious Diseases Authors: Cl áudia Fideles Resende Alison Miranda Dos Santos Paulo Martins Soares Filho Patr ícia Gomes de Souza Marina de Azevedo Issa Maur ício Baltazar de Carvalho Filho Raphael Mattoso Victor Rebeca J éssica Falcão Câmara Gilberto Pereira Gon çalves Juli Source Type: research
Glanders and brucellosis in equids from the Amazon region, Brazil
Acta Trop. 2022 Mar 25:106429. doi: 10.1016/j.actatropica.2022.106429. Online ahead of print.ABSTRACTGlanders and brucellosis are zoonotic infectious diseases that affect equids in several countries worldwide. On Marajó Island (Amazon region of Brazil), Marajoara and Puruca horses, which are well adapted to the climatic and territorial adversities of the region, play a fundamental role in the local economy and in the sociocultural lives of the population. However, these animals have undergone a drastic reduction in number, markedly due to precarious veterinary care, unknown causes of morbidity and mortality, and disordere...
Source: Acta Tropica - March 29, 2022 Category: Infectious Diseases Authors: Cl áudia Fideles Resende Alison Miranda Dos Santos Paulo Martins Soares Filho Patr ícia Gomes de Souza Marina de Azevedo Issa Maur ício Baltazar de Carvalho Filho Raphael Mattoso Victor Rebeca J éssica Falcão Câmara Gilberto Pereira Gon çalves Juli Source Type: research