Burkholderia mallei CLH001 Attenuated Vaccine Strain Is Immunogenic and Protects against Acute Respiratory Glanders [Microbial Immunity and Vaccines]
In this study, we constructed the select-agent-excluded B. mallei tonB hcp1 (CLH001) vaccine strain and investigated its ability to protect against acute respiratory glanders. Here we show that CLH001 is attenuated, safe, and effective at protecting against lethal B. mallei challenge. Intranasal administration of CLH001 to BALB/c and NOD SCID gamma (NSG) mice resulted in complete survival without detectable colonization or abnormal organ histopathology. Additionally, BALB/c mice intranasally immunized with CLH001 in a prime/boost regimen were fully protected against lethal challenge with the B. mallei lux (CSM001) wild-typ...
Source: Infection and Immunity - July 20, 2016 Category: Infectious Diseases Authors: Hatcher, C. L., Mott, T. M., Muruato, L. A., Sbrana, E., Torres, A. G. Tags: Microbial Immunity and Vaccines Source Type: research
Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species.
Abstract
The Burkholderia pseudomallei multilocus sequence typing (MLST) database (http://pubmlst.org/bpseudomallei/) contains the largest global sequence repository for B. pseudomallei and its closest genetic relatives. Using conventional MLST and in silico MLST data derived from publicly available whole-genome sequences, we first defined the phylogenetic relatedness of B. pseudomallei and its nearest neighbours. Based on this analysis, we propose that the recently described B. pseudomallei complex (Bpc) be expanded to encompass B. pseudomallei, B. humptydooensis (proposed), B. mallei, B. oklahomensis, B....
Source: Journal of Medical Microbiology - July 12, 2016 Category: Microbiology Authors: Price EP, MacHunter B, Spratt BG, Wagner DM, Currie BJ, Sarovich DS Tags: J Med Microbiol Source Type: research
Glanders and history
THE recent articles in Veterinary Record on testing for glanders in equids are to be welcomed (Laroucou and others 2016, Malik 2016). It is largely forgotten that this dreadful disease, also known in its cutaneous form as ‘farcy’, was only finally eradicated in Britain in 1928. It had a long and fearful history in this country, one of the first records being by Fitzherbert in his ‘Boke on Husbandry’ in 1523; it was also listed by Shakespeare as a familiar equine disorder (The Taming of the Shrew, Act III, Scene 2). It was almost certainly the cause of the early death in 1793 of Benoit Charles Vial, ...
Source: Veterinary Record - June 22, 2016 Category: Veterinary Research Authors: Jones, B. V. Tags: Letter Source Type: research
Developing Peptide Mimotopes of Capsular Polysaccharides and Lipopolysaccharides Protective Antigens of Pathogenic Burkholderia Bacteria.
In this study, we used phage display panning against three different phage peptide libraries to select phage clones specifically recognized by each of the four protective MAbs. After sequencing a total of 179 candidate phage clones, we examined in detail six selected phage clones carrying different peptide inserts for the specificity of binding by the respective target MAbs. Chemically synthesized peptides corresponding to those displayed by the six phage clones were conjugated to keyhole limpet hemocyanin carrier protein and tested for their binding specificity to the respective protective MAbs. The study revealed that fo...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 22, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research
Interlaboratory ring trial to evaluate CFT proficiency of European laboratories for diagnosis of glanders in equids
This study confirmed the reliability of CFT and highlighted its intralaboratory reproducibility. However, the sensitivity of glanders serodiagnosis and laboratory proficiency may be improved by standardising critical reagents, including antigens, and by developing a standard B mallei serum. (Source: Veterinary Record)
Source: Veterinary Record - June 15, 2016 Category: Veterinary Research Authors: Laroucau, K., Colaneri, C., Jaÿ, M., Corde, Y., Drapeau, A., Durand, B., Zientara, S., Beck, C., and European Union laboratories involved in glanders serodiagnosis Tags: Research Source Type: research
Harmonising diagnostic testing for glanders in equids
GLANDERS is a contagious and fatal disease in horses, donkeys and mules. It has been defined as an infection with Burkholderia mallei. It is considered a potentially lethal and dangerous zoonosis in people with a fatal outcome if not treated in a timely manner (DeShazer and Waag 2004). In equids the disease is among the oldest known and was described towards the beginning of recorded history. Hippocrates (460-370 BC) reported clinical signs of the disease in around 425 BC. Almost 100 years later, Aristotle (384-322 BC) documented glanders in donkeys in Historia Animalium (VIII, 25) (Leclainche 1936). The disease was also n...
Source: Veterinary Record - June 15, 2016 Category: Veterinary Research Authors: Malik, P. Tags: Research Source Type: research
Epidemiologic Status of Equine Viral Arteritis, Equine Infectious Anemia, and Glanders in Jordan
The objective of the present cross-sectional study was to determine whether antibodies against equine infectious anemia (EIA), equine viral arteritis (EVA), and Burkholderia mallei in horses from Jordan can be detected at all; moreover, the study aimed at identifying potential risk factors associated with a positive serologic result. Data related to management of each farm and individual horses were collected. Commercially available serologic kits were used to detect the presence of serum antibodies in 254 horses. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - April 17, 2016 Category: Veterinary Research Authors: Abdelsalam Q. Talafha, Sameeh M. Abutarbush, David L. Rutley Tags: Original Research Source Type: research
Epidemiologic status of equine viral arteritis, equine infectious anemia and glanders in Jordan
The objective of the present cross-sectional study was to determine if antibodies against EIA, EVA and Burkholderia mallei in horses from Jordan can be detected at all; moreover, the study aimed at identifying potential risk factors associated with a positive serological result. Data related to management of each farm and individual horses were collected. Commercially available serological kits were used to detect presence of serum antibodies in 254 horses. Potential risk factors associated with seropositivity were evaluated using the one-sided Chi square tests of association. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - April 17, 2016 Category: Veterinary Research Authors: Abdelsalam Q. Talafha, Sameeh M. Abutarbush, David L. Rutley Source Type: research
Development of safe, effective and immunogenic vaccine candidate for diarrheagenic Escherichia coli main pathotypes in mouse model
Background: Glanders is a highly contagious and fatal zoonotic disease primarily of horses, mules and may occur in felids in wild fields. At once time, B.mallei infection occurred worldwide, but over last 100 years occurrence decreased and many parts of world has been eradicated. However, sporadic cases of glanders are still registering in Far East, South America, North Africa, Middle East and Asia. In Mongolia, major glanders outbreaks were reported during the middle of last Century and due to specific measures, the prevalence was decreased to 0.05% by the end of 1980s. (Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - March 29, 2016 Category: Infectious Diseases Authors: O. Khurtsbaatar, B. Bayarsaikhan, V. Batbaatar, B. Bayartsetseg, J. Erdenebaatar Tags: Type: Poster Presentation Source Type: research
Phenotypic Characterization of a Novel Virulence-Factor Deletion Strain of Burkholderia mallei That Provides Partial Protection against Inhalational Glanders in Mice
Joel A. Bozue, Sidhartha Chaudhury, Kei Amemiya, Jennifer Chua, Christopher K. Cote, Ronald G. Toothman, Jennifer L. Dankmeyer, Christopher P. Klimko, Catherine L. Wilhelmsen, Jolynn W. Raymond, Nela Zavaljevski, Jaques Reifman, Anders Wallqvist (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - February 26, 2016 Category: Microbiology Source Type: research
Intracellular replication of the well-armed pathogen Burkholderia pseudomallei.
Abstract
The Burkholderia genus contains a group of soil-dwelling Gram-negative organisms that are prevalent in warm and humid climates. Two species in particular are able to cause disease in animals, B. mallei primarily infects Equus spp. and B. pseudomallei (BPS), that is able to cause potentially life-threatening disease in humans. BPS is naturally resistant to many antibiotics and there is no vaccine available. Although not a specialised human pathogen, BPS possesses a large genome and many virulence traits that allow it to adapt and survive very successfully in the human host. Key to this survival is ...
Source: Current Opinion in Microbiology - January 21, 2016 Category: Microbiology Authors: Willcocks SJ, Denman CC, Atkins HS, Wren BW Tags: Curr Opin Microbiol Source Type: research
Characterization of the murine macrophage response to infection with virulent and avirulent Burkholderia species
Conclusions:
These results characterize the phenotypic and immunological differences in the host innate response to pathogenic and avirulent Burkholderia strains and provide insight into the phenotypic alterations and molecular targets underlying host-Burkholderia interactions. (Source: BMC Microbiology)
Source: BMC Microbiology - November 6, 2015 Category: Microbiology Authors: Chih-Yuan ChiangRicky UlrichMelanie UlrichBrett EatonJenifer OjedaDouglas LaneKrishna KotaTara KennyJason LadnerSamuel DicksonKathleen KuehlRahul RaychaudhuriMei SunSina BavariMark WolcottDavid CovellRekha Panchal Source Type: research
Synthesis and characterization of biocompatibility of tenorite nanoparticles and potential property against biofilm formation
Publication date: September 2015 Source:Saudi Pharmaceutical Journal, Volume 23, Issue 4 Author(s): Davoodbasha MubarakAli, Jegatheesan Arunkumar, Pratheesh Pooja, Gopalakrishnan Subramanian, Nooruddin Thajuddin, Naiyf S Alharbi Aim is to assess the anti-biofilm property of tenorite nanoparticles and to study their suitability as a possible coating material for medical implants. Tenorite (CuO) nanoparticles were synthesized by the optimized thermal decomposition method and characterized using TEM, XRD, FTIR and UV–Vis analysis. Their influence on biofilm formation of microbes was studied by growing multi ...
Source: Saudi Pharmaceutical Journal - November 3, 2015 Category: Drugs & Pharmacology Source Type: research
Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial [Bacteriology]
In the case of a release of highly pathogenic bacteria (HPB), there is an urgent need for rapid, accurate, and reliable diagnostics. MALDI-TOF mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly important in microbiological diagnostics to complement classical microbiology, PCR, and genotyping of HPB. In the present study, the results of a joint exercise with 11 partner institutions from nine European countries are presented. In this exercise, 10 distinct microbial samples, among them five HPB, Bacillus anthracis, Brucella canis, Burkholderia mallei, Burkholderia pseudo...
Source: Journal of Clinical Microbiology - July 20, 2015 Category: Microbiology Authors: Lasch, P., Wahab, T., Weil, S., Palyi, B., Tomaso, H., Zange, S., Kiland Granerud, B., Drevinek, M., Kokotovic, B., Wittwer, M., Pfluger, V., Di Caro, A., Stammler, M., Grunow, R., Jacob, D. Tags: Bacteriology Source Type: research
Surveillance focus: glanders
The recent identification of a case of glanders in a horse in Germany that had never travelled outside of the country serves as a reminder that disease freedom cannot be taken for granted. Here, Sonia Gonzalez-Medina, Balazs Toth and Ian Mawhinney discuss various aspects of glanders, warning that, while an old disease, it can still surprise (Source: Veterinary Record)
Source: Veterinary Record - July 16, 2015 Category: Veterinary Research Authors: Gonzalez-Medina, S., Toth, B., Mawhinney, I. Tags: Surveillance Source Type: research
