Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species.

Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species. J Med Microbiol. 2016 Jul 13; Authors: Price EP, MacHunter B, Spratt BG, Wagner DM, Currie BJ, Sarovich DS Abstract The Burkholderia pseudomallei multilocus sequence typing (MLST) database (http://pubmlst.org/bpseudomallei/) contains the largest global sequence repository for B. pseudomallei and its closest genetic relatives. Using conventional MLST and in silico MLST data derived from publicly available whole-genome sequences, we first defined the phylogenetic relatedness of B. pseudomallei and its nearest neighbours. Based on this analysis, we propose that the recently described B. pseudomallei complex (Bpc) be expanded to encompass B. pseudomallei, B. humptydooensis (proposed), B. mallei, B. oklahomensis, B. thailandensis, and three unassigned Burkholderia Clades A, B and C (represented by type strains BDU 5, BDU 8, and MSMB0265, respectively). Of note, the MLST narK locus is present in all Bpc species but is missing in all other Burkholderia spp., including in all B. cepacia complex species, with the exception of most B. ubonensis strains, which contain narK but encode genetically distinct sequences. The presence of narK is thus indicative of a Bpc strain. Next, we revisited in silico the performance of the existing MLST primers, which prompted redesign of primers targeting the gmhD, lepA, lipA, narK and ndh loci to encompass genetic div...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research