Absence of SARS-CoV-2 in the air and on the surfaces within the school environment
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001424.ABSTRACTTo the best of our knowledge to date there are no scientific studies specifically investigating whether the SARS-CoV-2 virus is present in the air or on the various surfaces in the school environment. The aim of this study was to determine if SARS-CoV-2 is present on various high touch surfaces and in the air across the elementary, middle and high schools in the Chester County of Pennsylvania, USA. One hundred and fifty surface swab samples and 45 air samples were analysed for the presence of the virus. All the samples tested were negative for the presence ...
Source: Journal of Medical Microbiology - September 20, 2021 Category: Microbiology Authors: Amit Thakar Shawn Dutkiewicz Timothy Hoffman Paul Joyce Vishal Shah Source Type: research

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001367.ABSTRACTIntroduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis.Hypothesis/Gap Statement. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination.Aim.The diagnostic performance of the FMPS was evaluated and compared to that of tr...
Source: Journal of Medical Microbiology - September 13, 2021 Category: Microbiology Authors: Berta Fidalgo Elisa Rubio Victor Pastor Marta Parera Clara Ballest é-Delpierre Mariana Jos é Fernández Genoveva Cuesta Chasco Andrea Vergara Yuliya Zboromyrska Cristian Aylagas Pilar Salvador Ad án Fernández M Eugenia Valls M íriam José Alvarez Mar Source Type: research

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001367.ABSTRACTIntroduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis.Hypothesis/Gap Statement. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination.Aim.The diagnostic performance of the FMPS was evaluated and compared to that of tr...
Source: Journal of Medical Microbiology - September 13, 2021 Category: Microbiology Authors: Berta Fidalgo Elisa Rubio Victor Pastor Marta Parera Clara Ballest é-Delpierre Mariana Jos é Fernández Genoveva Cuesta Chasco Andrea Vergara Yuliya Zboromyrska Cristian Aylagas Pilar Salvador Ad án Fernández M Eugenia Valls M íriam José Alvarez Mar Source Type: research

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001367.ABSTRACTIntroduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis.Hypothesis/Gap Statement. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination.Aim.The diagnostic performance of the FMPS was evaluated and compared to that of tr...
Source: Journal of Medical Microbiology - September 13, 2021 Category: Microbiology Authors: Berta Fidalgo Elisa Rubio Victor Pastor Marta Parera Clara Ballest é-Delpierre Mariana Jos é Fernández Genoveva Cuesta Chasco Andrea Vergara Yuliya Zboromyrska Cristian Aylagas Pilar Salvador Ad án Fernández M Eugenia Valls M íriam José Alvarez Mar Source Type: research

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001367.ABSTRACTIntroduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis.Hypothesis/Gap Statement. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination.Aim.The diagnostic performance of the FMPS was evaluated and compared to that of tr...
Source: Journal of Medical Microbiology - September 13, 2021 Category: Microbiology Authors: Berta Fidalgo Elisa Rubio Victor Pastor Marta Parera Clara Ballest é-Delpierre Mariana Jos é Fernández Genoveva Cuesta Chasco Andrea Vergara Yuliya Zboromyrska Cristian Aylagas Pilar Salvador Ad án Fernández M Eugenia Valls M íriam José Alvarez Mar Source Type: research

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001367.ABSTRACTIntroduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis.Hypothesis/Gap Statement. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination.Aim.The diagnostic performance of the FMPS was evaluated and compared to that of tr...
Source: Journal of Medical Microbiology - September 13, 2021 Category: Microbiology Authors: Berta Fidalgo Elisa Rubio Victor Pastor Marta Parera Clara Ballest é-Delpierre Mariana Jos é Fernández Genoveva Cuesta Chasco Andrea Vergara Yuliya Zboromyrska Cristian Aylagas Pilar Salvador Ad án Fernández M Eugenia Valls M íriam José Alvarez Mar Source Type: research

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001367.ABSTRACTIntroduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis.Hypothesis/Gap Statement. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination.Aim.The diagnostic performance of the FMPS was evaluated and compared to that of tr...
Source: Journal of Medical Microbiology - September 13, 2021 Category: Microbiology Authors: Berta Fidalgo Elisa Rubio Victor Pastor Marta Parera Clara Ballest é-Delpierre Mariana Jos é Fernández Genoveva Cuesta Chasco Andrea Vergara Yuliya Zboromyrska Cristian Aylagas Pilar Salvador Ad án Fernández M Eugenia Valls M íriam José Alvarez Mar Source Type: research

Challenges on the development of a pseudotyping assay for Zika glycoproteins
Conclusion. In line with other reports, we were unable to successfully produce infectious pseudotypes using the variety of methods described. Other strategies may be more suitable in the development of an efficient pseudotype model for ZIKV and other flaviviruses.PMID:34499027 | DOI:10.1099/jmm.0.001413 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 9, 2021 Category: Microbiology Authors: Fernando Ruiz-Jim énez Jose Humberto P érez-Olais Chidinma Raymond Barnabas J King C Patrick McClure Richard A Urbanowicz Jonathan K Ball Source Type: research

Challenges on the development of a pseudotyping assay for Zika glycoproteins
Conclusion. In line with other reports, we were unable to successfully produce infectious pseudotypes using the variety of methods described. Other strategies may be more suitable in the development of an efficient pseudotype model for ZIKV and other flaviviruses.PMID:34499027 | DOI:10.1099/jmm.0.001413 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 9, 2021 Category: Microbiology Authors: Fernando Ruiz-Jim énez Jose Humberto P érez-Olais Chidinma Raymond Barnabas J King C Patrick McClure Richard A Urbanowicz Jonathan K Ball Source Type: research

Challenges on the development of a pseudotyping assay for Zika glycoproteins
Conclusion. In line with other reports, we were unable to successfully produce infectious pseudotypes using the variety of methods described. Other strategies may be more suitable in the development of an efficient pseudotype model for ZIKV and other flaviviruses.PMID:34499027 | DOI:10.1099/jmm.0.001413 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 9, 2021 Category: Microbiology Authors: Fernando Ruiz-Jim énez Jose Humberto P érez-Olais Chidinma Raymond Barnabas J King C Patrick McClure Richard A Urbanowicz Jonathan K Ball Source Type: research

Adult gonococcal conjunctivitis: Prevalence, clinical features and complications
Conclusion. AGC is a rare disease which is difficult to diagnose as it requires a high index of suspicion to prevent corneal perforation but in an important number of cases it may mimic orbital cellulitis. It is crucial that treatment starts as soon as possible to avoid serious corneal damage. Patients should promptly receive complete and correct treatment when admitted to the emergency room since an elevated number of patients do not attend their medical follow-up visit. Azithromycin or aminoglycoside eye drops are probably the best option to complete the treatment, due to high quinolone resistance.PMID:34494952 | DOI:10....
Source: Journal of Medical Microbiology - September 8, 2021 Category: Microbiology Authors: Genoveva Cuesta Chasco Xavier Carreras-Casta ñer Yuliya Zboromyrska Cristina Pitart Francisco Palma-Carvajal Jordi Bosch Juan Pablo Figueroa-Vercellino Source Type: research

< em > In vitro < /em > antifungal effect of a plant-based product, CIN-102, on antifungal resistant filamentous fungi and their biofilms
Conclusions. In this study we show that CIN-102 is effective against resistant filamentous fungi and against biofilm formation. In addition, our strains did not acquire a resistance phenotype against CIN-102 over time, unlike with VRC. CIN-102 is therefore an interesting candidate for the treatment of IFIs, including in cases of therapeutic failure linked to resistance, although further studies on its efficacy, safety and mechanism of action are needed.PMID:34491156 | DOI:10.1099/jmm.0.001399 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 7, 2021 Category: Microbiology Authors: Maurine D'agostino Nicolas Tesse Rose Anne Lavergne Patrice Le Pape Jean Pol Frippiat Marie Machouart Anne Debourgogne Source Type: research

Sample collection and transport strategies to enhance yield, accessibility, and biosafety of COVID-19 RT-PCR testing
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001380.ABSTRACTIntroduction. Non-invasive sample collection and viral sterilizing buffers have independently enabled workflows for more widespread COVID-19 testing by reverse-transcriptase polymerase chain reaction (RT-PCR).Gap statement. The combined use of sterilizing buffers across non-invasive sample types to optimize sensitive, accessible, and biosafe sampling methods has not been directly and systematically compared.Aim. We aimed to evaluate diagnostic yield across different non-invasive samples with standard viral transport media (VTM) versus a sterilizing buffer e...
Source: Journal of Medical Microbiology - September 6, 2021 Category: Microbiology Authors: Padmapriya Banada David Elson Naranjargal Daivaa Claire Park Samuel Desind Ibsen Montalvan Robert Kwiatkowski Soumitesh Chakravorty David Alland Yingda L Xie Source Type: research

< em > Prototheca bovis, < /em > a unicellular achlorophyllous trebouxiophyte green alga in the healthy human intestine
Conclusion. The results represent the first molecular characterization of Prototheca in Thailand. The study provides insight into transmission dynamics of the organism and potential caveats in estimating the global prevalence of Prototheca. These will spearhead further investigations on Prototheca occurrence in rural areas of both industrialized and developing nations.PMID:34486973 | DOI:10.1099/jmm.0.001415 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 6, 2021 Category: Microbiology Authors: Vasana Jinatham Diego M Cantoni Ian R Brown Thanakrit Vichaslip Picha Suwannahitatorn Siam Popluechai Anastasios D Tsaousis Eleni Gentekaki Source Type: research

Sickle cell disease children's gut colonization by extended-spectrum β-lactamase (ESBL)-producing < em > Enterobacterales < /em > : an antibiotic prophylaxis effect?
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001414.ABSTRACTIntroduction. Sickle cell disease (SCD) children have a high susceptibility to pneumococcal infection. For this reason, they are routinely immunized with pneumococcal vaccines and use antibiotic prophylaxis (AP).Hypothesis/Gap Statement. Yet, little is known about SCD children's gut microbiota. If antibiotic-resistant Enterobacterales may colonize people on AP, we hypothesized that SCD children on AP are colonized by resistant enterobacteria species.Objective. To evaluate the effect of continuous AP on Enterobacterales gut colonization from children with SC...
Source: Journal of Medical Microbiology - September 3, 2021 Category: Microbiology Authors: Adriano de Souza Santos Monteiro Eduardo Gomes de Oliveira Djanilson Barbosa Dos Santos Soraia Machado Cordeiro Ricardo David Couto F ábio David Couto Source Type: research

Molecular characterization of rectal isolates of carbapenemase-negative carbapenem-resistant enterobacterales obtained from ICU patients in Kuwait by whole-genome sequencing
Conclusions. This finding reflects the superior detection capabilities offered by WGS analysis, which can be used to complement traditional methods and overcome their limited resolution in clinical settings.PMID:34477546 | DOI:10.1099/jmm.0.001409 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 3, 2021 Category: Microbiology Authors: Amani Al Fadhli Wafaa Jamal Vincent O Rotimi Source Type: research

Disinfection to control African swine fever virus: a UK perspective
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001410.ABSTRACTA review of African swine fever (ASF) was conducted, including manifestations of disease, its transmission and environmental persistence of ASF virus. Findings on infectious doses of contemporary highly-pathogenic strains isolated from outbreaks in Eastern Europe were included. Published data on disinfectant susceptibility of ASF virus were then compared with similar findings for selected other infectious agents, principally those used in the UK disinfectant approvals tests relating to relevant Disease Orders for the control of notifiable and zoonotic disea...
Source: Journal of Medical Microbiology - September 3, 2021 Category: Microbiology Authors: Andrew D Wales Robert H Davies Source Type: research

To zinc or not to zinc for COVID-19 prophylaxis or treatment?
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001299.NO ABSTRACTPMID:34468306 | DOI:10.1099/jmm.0.001299 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 1, 2021 Category: Microbiology Authors: Kate Chander Chiang Ajay Gupta Source Type: research

An update on ampicillin resistance and β-lactamase genes of < em > Bacteroides < /em > spp
Conclusion. cepA usually is not activated by IS elements and usually produces low β-lactamase activities that do not correlate with the ampicillin MICs; therefore, it probably involves some non-β-lactamase-mediated resistance mechanism(s). pbpA is a newly described, effective β-lactamase gene that is located on a plasmid, and cepA resides on a well-defined chromosomal segment that is mutually replaced in division II B. fragilis strains. This latter finding demonstrates the genetic dichotomy of cepA-cfiA in B. fragilis and requires further investigation.PMID:34463608 | DOI:10.1099/jmm.0.001393 (Source: Journa...
Source: Journal of Medical Microbiology - August 31, 2021 Category: Microbiology Authors: J ózsef Sóki Anik ó Keszőcze Istv án Nagy Katalin Buri án Elisabeth Nagy Source Type: research

Pan-drug resistant < em > Providencia rettgeri < /em > contributing to a fatal case of COVID-19
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001406.ABSTRACTFollowing prolonged hospitalization that included broad-spectrum antibiotic exposure, a strain of Providencia rettgeri was cultured from the blood of a patient undergoing extracorporeal membrane oxygenation treatment for hypoxic respiratory failure due to COVID-19. The strain was resistant to all antimicrobials tested including the novel siderophore cephalosporin, cefiderocol. Whole genome sequencing detected ten antimicrobial resistance genes, including the metallo-β-lactamase bla NDM-1, the extended-spectrum β-lactamase bla PER-1, and the rare 1...
Source: Journal of Medical Microbiology - August 27, 2021 Category: Microbiology Authors: Patrick Mc Gann Matthew R Geringer Lindsey R Hall Francois Lebreton Elizabeth Markelz Yoon I Kwak Sheila Johnson Ana C Ong Aubrey Powell Tsigereda Tekle Yehudit Bergman Patricia J Simner Jason W Bennett Robert J Cybulski Brian K White Source Type: research

Pan-drug resistant < em > Providencia rettgeri < /em > contributing to a fatal case of COVID-19
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001406.ABSTRACTFollowing prolonged hospitalization that included broad-spectrum antibiotic exposure, a strain of Providencia rettgeri was cultured from the blood of a patient undergoing extracorporeal membrane oxygenation treatment for hypoxic respiratory failure due to COVID-19. The strain was resistant to all antimicrobials tested including the novel siderophore cephalosporin, cefiderocol. Whole genome sequencing detected ten antimicrobial resistance genes, including the metallo-β-lactamase bla NDM-1, the extended-spectrum β-lactamase bla PER-1, and the rare 1...
Source: Journal of Medical Microbiology - August 27, 2021 Category: Microbiology Authors: Patrick Mc Gann Matthew R Geringer Lindsey R Hall Francois Lebreton Elizabeth Markelz Yoon I Kwak Sheila Johnson Ana C Ong Aubrey Powell Tsigereda Tekle Yehudit Bergman Patricia J Simner Jason W Bennett Robert J Cybulski Brian K White Source Type: research

Pan-drug resistant < em > Providencia rettgeri < /em > contributing to a fatal case of COVID-19
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001406.ABSTRACTFollowing prolonged hospitalization that included broad-spectrum antibiotic exposure, a strain of Providencia rettgeri was cultured from the blood of a patient undergoing extracorporeal membrane oxygenation treatment for hypoxic respiratory failure due to COVID-19. The strain was resistant to all antimicrobials tested including the novel siderophore cephalosporin, cefiderocol. Whole genome sequencing detected ten antimicrobial resistance genes, including the metallo-β-lactamase bla NDM-1, the extended-spectrum β-lactamase bla PER-1, and the rare 1...
Source: Journal of Medical Microbiology - August 27, 2021 Category: Microbiology Authors: Patrick Mc Gann Matthew R Geringer Lindsey R Hall Francois Lebreton Elizabeth Markelz Yoon I Kwak Sheila Johnson Ana C Ong Aubrey Powell Tsigereda Tekle Yehudit Bergman Patricia J Simner Jason W Bennett Robert J Cybulski Brian K White Source Type: research

Genomic epidemiology of the opportunistic pathogen < em > Staphylococcus coagulans < /em > from companion dogs
Conclusion. The availability of a further 25 genome sequences from clinical S. coagulans isolates will aid in better understanding the epidemiology, bacterial-host interactions and antimicrobial resistance of this opportunistic pathogen.PMID:34431760 | DOI:10.1099/jmm.0.001407 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Gavin K Paterson Source Type: research

Emergence of a multidrug-resistant plasmid encoding < em > bla < /em > < sub > NDM-1 < /sub > , < em > bla < /em > < sub > OXA-420 < /sub > and < em > armA < /em > in a clinical isolate of < em > Acinetobacter variabilis < /em > in Japan
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001395.ABSTRACTAcinetobacter variabilis (formerly genospecies 15 sensu Tjernberg and Ursing) has been isolated from humans and animals and was proposed to be a novel species in 2015. A multidrug-resistant A. variabilis isolate, RYU24, was obtained in 2012 from an inpatient in Okinawa, Japan, with no record of overseas travel. The isolate was resistant to carbapenems, aminoglycosides and ciprofloxacin, with minimum inhibitory concentrations (MICs) of 32 µg ml-1 for imipenem and meropenem;> 1024 µg ml-1 for amikacin, arbekacin, gentamicin and tobramycin; and ...
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Kohei Uechi Mari Tohya Tatsuya Tada Takaaki Tome Ami Takahashi Takeshi Kinjo Shiro Maeda Teruo Kirikae Jiro Fujita Source Type: research

The effect of probiotic supplementation on the gut microbiota of preterm infants
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001403.ABSTRACTIntroduction. Probiotic supplementation of preterm infants may prevent serious morbidities associated with prematurity.Aim. To investigate the impact of probiotic supplementation on the gut microbiota and determine factors associated with detection of probiotic species in the infant gut.Hypothesis/Gap Statement. Probiotic supplementation increases the long-term colonization of probiotic species in the gut of preterm infants.Methodology. Longitudinal stool samples were collected from a cohort of very preterm infants participating in a blinded randomized cont...
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Erica L Plummer Jennifer A Danielewski Suzanne M Garland Jenny Su Susan E Jacobs Gerald L Murray Source Type: research

Risk factors for isolation of fluconazole and echinocandin non-susceptible < em > Candida < /em > species in critically ill patients
Conclusion. Fluconazole's non-susceptibility increased during the study period, despite the decrease of its administration. Although echinocandins' administration remained stable, non-susceptibility among Candida spp. increased.PMID:34431765 | DOI:10.1099/jmm.0.001401 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Matthaios Papadimitriou-Olivgeris Anastasia Spiliopoulou Fotini Fligou Ekaterini Tsiata Fevronia Kolonitsiou Alexandra Nikolopoulou Chrysavgi Papamichail Iris Spiliopoulou Markos Marangos Myrto Christofidou Source Type: research

Genomic epidemiology of the opportunistic pathogen < em > Staphylococcus coagulans < /em > from companion dogs
Conclusion. The availability of a further 25 genome sequences from clinical S. coagulans isolates will aid in better understanding the epidemiology, bacterial-host interactions and antimicrobial resistance of this opportunistic pathogen.PMID:34431760 | DOI:10.1099/jmm.0.001407 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Gavin K Paterson Source Type: research

Emergence of a multidrug-resistant plasmid encoding < em > bla < /em > < sub > NDM-1 < /sub > , < em > bla < /em > < sub > OXA-420 < /sub > and < em > armA < /em > in a clinical isolate of < em > Acinetobacter variabilis < /em > in Japan
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001395.ABSTRACTAcinetobacter variabilis (formerly genospecies 15 sensu Tjernberg and Ursing) has been isolated from humans and animals and was proposed to be a novel species in 2015. A multidrug-resistant A. variabilis isolate, RYU24, was obtained in 2012 from an inpatient in Okinawa, Japan, with no record of overseas travel. The isolate was resistant to carbapenems, aminoglycosides and ciprofloxacin, with minimum inhibitory concentrations (MICs) of 32 µg ml-1 for imipenem and meropenem;> 1024 µg ml-1 for amikacin, arbekacin, gentamicin and tobramycin; and ...
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Kohei Uechi Mari Tohya Tatsuya Tada Takaaki Tome Ami Takahashi Takeshi Kinjo Shiro Maeda Teruo Kirikae Jiro Fujita Source Type: research

The effect of probiotic supplementation on the gut microbiota of preterm infants
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001403.ABSTRACTIntroduction. Probiotic supplementation of preterm infants may prevent serious morbidities associated with prematurity.Aim. To investigate the impact of probiotic supplementation on the gut microbiota and determine factors associated with detection of probiotic species in the infant gut.Hypothesis/Gap Statement. Probiotic supplementation increases the long-term colonization of probiotic species in the gut of preterm infants.Methodology. Longitudinal stool samples were collected from a cohort of very preterm infants participating in a blinded randomized cont...
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Erica L Plummer Jennifer A Danielewski Suzanne M Garland Jenny Su Susan E Jacobs Gerald L Murray Source Type: research

Risk factors for isolation of fluconazole and echinocandin non-susceptible < em > Candida < /em > species in critically ill patients
Conclusion. Fluconazole's non-susceptibility increased during the study period, despite the decrease of its administration. Although echinocandins' administration remained stable, non-susceptibility among Candida spp. increased.PMID:34431765 | DOI:10.1099/jmm.0.001401 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 25, 2021 Category: Microbiology Authors: Matthaios Papadimitriou-Olivgeris Anastasia Spiliopoulou Fotini Fligou Ekaterini Tsiata Fevronia Kolonitsiou Alexandra Nikolopoulou Chrysavgi Papamichail Iris Spiliopoulou Markos Marangos Myrto Christofidou Source Type: research

Corrigendum: Comparative genome analysis of three Group A < em > Streptococcus dysgalactiae < /em > subsp. < em > equisimilis < /em > strains isolated in Japan
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001390.NO ABSTRACTPMID:34406921 | DOI:10.1099/jmm.0.001390 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 18, 2021 Category: Microbiology Authors: Haruka Ishihara Kohei Ogura Van An Nguyen Tohru Miyoshi-Akiyama Shigefumi Okamoto Norihiko Takemoto Source Type: research

Corrigendum: Comparative genome analysis of three Group A < em > Streptococcus dysgalactiae < /em > subsp. < em > equisimilis < /em > strains isolated in Japan
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001390.NO ABSTRACTPMID:34406921 | DOI:10.1099/jmm.0.001390 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 18, 2021 Category: Microbiology Authors: Haruka Ishihara Kohei Ogura Van An Nguyen Tohru Miyoshi-Akiyama Shigefumi Okamoto Norihiko Takemoto Source Type: research

Corrigendum: Comparative genome analysis of three Group A < em > Streptococcus dysgalactiae < /em > subsp. < em > equisimilis < /em > strains isolated in Japan
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001390.NO ABSTRACTPMID:34406921 | DOI:10.1099/jmm.0.001390 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 18, 2021 Category: Microbiology Authors: Haruka Ishihara Kohei Ogura Van An Nguyen Tohru Miyoshi-Akiyama Shigefumi Okamoto Norihiko Takemoto Source Type: research

Corrigendum: Comparative genome analysis of three Group A < em > Streptococcus dysgalactiae < /em > subsp. < em > equisimilis < /em > strains isolated in Japan
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001390.NO ABSTRACTPMID:34406921 | DOI:10.1099/jmm.0.001390 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 18, 2021 Category: Microbiology Authors: Haruka Ishihara Kohei Ogura Van An Nguyen Tohru Miyoshi-Akiyama Shigefumi Okamoto Norihiko Takemoto Source Type: research

Corrigendum: Comparative genome analysis of three Group A < em > Streptococcus dysgalactiae < /em > subsp. < em > equisimilis < /em > strains isolated in Japan
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001390.NO ABSTRACTPMID:34406921 | DOI:10.1099/jmm.0.001390 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 18, 2021 Category: Microbiology Authors: Haruka Ishihara Kohei Ogura Van An Nguyen Tohru Miyoshi-Akiyama Shigefumi Okamoto Norihiko Takemoto Source Type: research

High ESBL-E colonization rate among children in Gabon: a follow-up study
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001405.ABSTRACTA previous study conducted in Gabon, Central Africa, in 2010/11 found a high colonization rate with extended-spectrum β-lactamase-producing enterobacterales (ESBL-E) among children of ~34 %. Eight years later, we aimed to reassess the ESBL-E rate and previously identified risk factors for colonization in children from Gabon. We conducted a cross-sectional cohort study in 2018 on 92 outpatients under 5 years of age with diarrhoea in Lambaréné, Gabon, in whom a rectal swab was obtained at the initial medical encounter (baseline). Fifty-eigh...
Source: Journal of Medical Microbiology - August 17, 2021 Category: Microbiology Authors: Philipp Hofmann Abraham Alabi G édéon P Manouana Francis C Onwugamba Amelia Hasenauer Maradona D Agbanrin Christiane S M Gouleu G édéon Bingoulou Steffen Borrmann Matthew B B McCall Ayola A Adegnika Source Type: research

High ESBL-E colonization rate among children in Gabon: a follow-up study
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001405.ABSTRACTA previous study conducted in Gabon, Central Africa, in 2010/11 found a high colonization rate with extended-spectrum β-lactamase-producing enterobacterales (ESBL-E) among children of ~34 %. Eight years later, we aimed to reassess the ESBL-E rate and previously identified risk factors for colonization in children from Gabon. We conducted a cross-sectional cohort study in 2018 on 92 outpatients under 5 years of age with diarrhoea in Lambaréné, Gabon, in whom a rectal swab was obtained at the initial medical encounter (baseline). Fifty-eigh...
Source: Journal of Medical Microbiology - August 17, 2021 Category: Microbiology Authors: Philipp Hofmann Abraham Alabi G édéon P Manouana Francis C Onwugamba Amelia Hasenauer Maradona D Agbanrin Christiane S M Gouleu G édéon Bingoulou Steffen Borrmann Matthew B B McCall Ayola A Adegnika Source Type: research

Immunogenicity after the first dose of the BNT162b2 mRNA Covid-19 vaccine: real-world evidence from Greek healthcare workers
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001387.ABSTRACTReal-world data regarding the effectiveness, safety and immunogenicity of the Pfizer-BioNTech BNT162b2 mRNA vaccine are accumulating in the literature, suggesting that this vaccine generates high titres of S1-binding IgG antibodies that exhibit potent virus neutralization capacity. This is the first phase IV immunogenicity study to recruit a large number of Greek healthcare workers (n=425) including 63 previously-infected subjects. We measured titres of neutralizing IgGs against the receptor-binding domain of the S1 subunit of the spike protein of SARS-CoV-...
Source: Journal of Medical Microbiology - August 16, 2021 Category: Microbiology Authors: Konstantina Kontopoulou Alexandra Ainatzoglou Athina Ifantidou Christos T Nakas Georgia Gkounti Vasilios Adamopoulos Nikitas Papadopoulos Georgios Papazisis Source Type: research

Complete genome sequence and probiotic properties of < em > Lactococcus petauri < /em > LZys1 isolated from healthy human gut
Conclusion. All the results described in this study, together with relevant information previously reported, made L. prtauri LZys1 a very interesting potential strain to be considered as a prominent candidate for probiotic use.PMID:34397349 | DOI:10.1099/jmm.0.001397 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2021 Category: Microbiology Authors: Ouyang Li Huijian Zhang Wenjing Wang Yuxin Liang Wenbi Chen Ahmad Ud Din Li Li Yingshun Zhou Source Type: research

Validation of saliva sampling as an alternative to oro-nasopharyngeal swab for detection of SARS-CoV-2 using unextracted rRT-PCR with the Allplex 2019-nCoV assay
Conclusions. Saliva sampling is an acceptable alternative to ONPS for diagnosing SARS-CoV-2 infection in symptomatic individuals displaying symptoms for ≤ 10 days. These results reinforce the need to expand the use of saliva samples, which are self-collected and do not require swabs.PMID:34369860 | DOI:10.1099/jmm.0.001404 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 9, 2021 Category: Microbiology Authors: Marco Andres Bergevin Wesley Freppel Guylaine Robert Georges Ambaraghassi Dany Aubry Olivier Haeck Maude Saint-Jean Alex Carignan Source Type: research

Validation of saliva sampling as an alternative to oro-nasopharyngeal swab for detection of SARS-CoV-2 using unextracted rRT-PCR with the Allplex 2019-nCoV assay
Conclusions. Saliva sampling is an acceptable alternative to ONPS for diagnosing SARS-CoV-2 infection in symptomatic individuals displaying symptoms for ≤ 10 days. These results reinforce the need to expand the use of saliva samples, which are self-collected and do not require swabs.PMID:34369860 | DOI:10.1099/jmm.0.001404 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 9, 2021 Category: Microbiology Authors: Marco Andres Bergevin Wesley Freppel Guylaine Robert Georges Ambaraghassi Dany Aubry Olivier Haeck Maude Saint-Jean Alex Carignan Source Type: research

Validation of saliva sampling as an alternative to oro-nasopharyngeal swab for detection of SARS-CoV-2 using unextracted rRT-PCR with the Allplex 2019-nCoV assay
Conclusions. Saliva sampling is an acceptable alternative to ONPS for diagnosing SARS-CoV-2 infection in symptomatic individuals displaying symptoms for ≤ 10 days. These results reinforce the need to expand the use of saliva samples, which are self-collected and do not require swabs.PMID:34369860 | DOI:10.1099/jmm.0.001404 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 9, 2021 Category: Microbiology Authors: Marco Andres Bergevin Wesley Freppel Guylaine Robert Georges Ambaraghassi Dany Aubry Olivier Haeck Maude Saint-Jean Alex Carignan Source Type: research

Validation of saliva sampling as an alternative to oro-nasopharyngeal swab for detection of SARS-CoV-2 using unextracted rRT-PCR with the Allplex 2019-nCoV assay
Conclusions. Saliva sampling is an acceptable alternative to ONPS for diagnosing SARS-CoV-2 infection in symptomatic individuals displaying symptoms for ≤ 10 days. These results reinforce the need to expand the use of saliva samples, which are self-collected and do not require swabs.PMID:34369860 | DOI:10.1099/jmm.0.001404 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 9, 2021 Category: Microbiology Authors: Marco Andres Bergevin Wesley Freppel Guylaine Robert Georges Ambaraghassi Dany Aubry Olivier Haeck Maude Saint-Jean Alex Carignan Source Type: research

Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001402.ABSTRACTSeveral studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and s...
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Farah M Shurrab Duaa W Al-Sadeq Fathima Amanullah Salma N Younes Hadeel Al-Jighefee Nadin Younes Soha R Dargham Hadi M Yassine Laith J Abu Raddad Gheyath K Nasrallah Source Type: research

Validation of Pefloxacin for detection of fluoroquinolone (FQ) resistance among < em > Salmonella < /em > Typhi with special reference to GyrB mutations
Conclusion. The pefloxacin disc can be used to detect FQ resistance among S. Typhi. This is the first report of validation of pefloxacin for detection of FQ resistance in S. Typhi mediated by GyrB mutation.PMID:34356003 | DOI:10.1099/jmm.0.001398 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Shakila Banu Inayath Shobha Broor Ruchi Gupta Priti Agarwal Subhradeep Majumder Anupam Kumar Anveshi Rajni Gaind Source Type: research

Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001402.ABSTRACTSeveral studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and s...
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Farah M Shurrab Duaa W Al-Sadeq Fathima Amanullah Salma N Younes Hadeel Al-Jighefee Nadin Younes Soha R Dargham Hadi M Yassine Laith J Abu Raddad Gheyath K Nasrallah Source Type: research

Validation of Pefloxacin for detection of fluoroquinolone (FQ) resistance among < em > Salmonella < /em > Typhi with special reference to GyrB mutations
Conclusion. The pefloxacin disc can be used to detect FQ resistance among S. Typhi. This is the first report of validation of pefloxacin for detection of FQ resistance in S. Typhi mediated by GyrB mutation.PMID:34356003 | DOI:10.1099/jmm.0.001398 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Shakila Banu Inayath Shobha Broor Ruchi Gupta Priti Agarwal Subhradeep Majumder Anupam Kumar Anveshi Rajni Gaind Source Type: research

Increasingly limited options for the treatment of enteric fever in travellers returning to England, 2014-2019: a cross-sectional analytical study
Conclusion. Third-generation cephalosporins and azithromycin remain appropriate choices for empirical treatment of enteric fever in most returning travellers to the UK from endemic countries, except from Pakistan, where XDR represents a significant risk.PMID:34351258 | DOI:10.1099/jmm.0.001359 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 5, 2021 Category: Microbiology Authors: M Trent Herdman Basel Karo Jayshree Dave Parisha Katwa Joanne Freedman Vivienne Do Nascimento Hilary Kirkbride Marie Anne Chattaway Gauri Godbole Sooria Balasegaram Source Type: research

Increasingly limited options for the treatment of enteric fever in travellers returning to England, 2014-2019: a cross-sectional analytical study
Conclusion. Third-generation cephalosporins and azithromycin remain appropriate choices for empirical treatment of enteric fever in most returning travellers to the UK from endemic countries, except from Pakistan, where XDR represents a significant risk.PMID:34351258 | DOI:10.1099/jmm.0.001359 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 5, 2021 Category: Microbiology Authors: M Trent Herdman Basel Karo Jayshree Dave Parisha Katwa Joanne Freedman Vivienne Do Nascimento Hilary Kirkbride Marie Anne Chattaway Gauri Godbole Sooria Balasegaram Source Type: research

Increased biofilm formation by < em > Staphylococcus aureus < /em > clinical isolates on surfaces covered with plasma proteins
Conclusion. An increase in biofilm production in the presence of plasma proteins highlights the importance of investigating biofilm formation by S. aureus clinical isolates under different conditions since this virulence factor contributes to persistent infections and increased resistance to antimicrobials.PMID:34338626 | DOI:10.1099/jmm.0.001389 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 2, 2021 Category: Microbiology Authors: Lorrayne Cardoso Guimar ães Bruna Marques de Souza Carolina de Oliveira Whitaker Fernanda Abreu Rosana Barreto Rocha Ferreira K átia Regina Netto Dos Santos Source Type: research