Komodo-dragon cathelicidin-inspired peptides are antibacterial against carbapenem-resistant Klebsiella pneumoniae.
Conclusion. Given the challenges of treating CRKP, identification of peptides with activity against it represents a promising avenue for further research. Given DRGN-6's similar level of activity to colistin, DRGN-6 is a promising template for the development of novel antimicrobial peptide-based therapeutics. PMID: 33084564 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 21, 2020 Category: Microbiology Authors: Hitt SJ, Bishop BM, van Hoek ML Tags: J Med Microbiol Source Type: research

Molecular evaluation of quorum quenching potential of vanillic acid against Yersinia enterocolitica through transcriptomic and in silico analysis.
Conclusion. The present study is possibly the first attempt to demonstrate the anti-QS and anti-pathogenic potential of VA against Y. enterocolitica by transcriptomic and in silico analysis. It also deciphers that VA can be a promising lead to develop biopreservative and therapeutic regimens to treat Y. enterocolitica infections. PMID: 33084565 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 21, 2020 Category: Microbiology Authors: Sivasankar C, Jha NK, Singh SR, Murali A, Shetty PH Tags: J Med Microbiol Source Type: research

Comparison of methods for the microbiological diagnosis of totally implantable venous access port-related infections.
Abstract Introduction. Totally implanted venous access ports (TIVAPs) are widely used in patients receiving long-term chemotherapy but may lead to serious complications such as catheter-related bloodstream infections (CRBSIs). Diagnosis of CRBSI requires catheter culture, but there is no consensus on microbiological culture methods to be adopted.Aim. To compare three different procedures to recover bacterial cells from colonized catheters and to determine which section of the TIVAP (i.e. tip, septum, reservoir) is the probable source of infection. To investigate the correlation between blood culture results and TI...
Source: Journal of Medical Microbiology - October 16, 2020 Category: Microbiology Authors: Kaya E, Tollapi L, Pastore A, Aringhieri G, Maisetta G, Barnini S, Paolicchi A, Batoni G, Esin S Tags: J Med Microbiol Source Type: research

Whole-genome sequencing to investigate two concurrent outbreaks of Salmonella Enteritidis in South Africa, 2018.
We describe the use of whole-genome sequencing (WGS) analysis of bacterial isolates to assist with the investigation of these outbreaks. Outbreak A affected children (n=27) attending a day-care centre, while outbreak B affected adults (n=16) who ate breakfast at the same restaurant. Salmonella Enteritidis was isolated from stool samples in both outbreaks (four children in outbreak A; 12 restaurant customers and three restaurant food-handlers in outbreak B). In outbreak B, Salmonella Enteritidis was isolated from three food retention samples (raw chicken egg, hollandaise sauce and rocket-herb). Available isolates from both ...
Source: Journal of Medical Microbiology - October 13, 2020 Category: Microbiology Authors: Smith AM, Tau NP, Ngomane HM, Sekwadi P, Ramalwa N, Moodley K, Govind C, Khan S, Archary M, Thomas J Tags: J Med Microbiol Source Type: research

Comparing DADA2 and OTU clustering approaches in studying the bacterial communities of atopic dermatitis.
Conclusion. We believe that DADA2 represents an improvement over an OTU clustering approach, and that biological replication rather than technical replication is a more effective use of resources. However, neither OTU clustering nor DADA2 gave insights into Staphylococcus community dynamics, and caution should remain in not overinterpreting the taxonomic assignments at lower taxonomic ranks. PMID: 32965212 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 22, 2020 Category: Microbiology Authors: Barnes CJ, Rasmussen L, Asplund M, Knudsen SW, Clausen ML, Agner T, Hansen AJ Tags: J Med Microbiol Source Type: research

Molecular identification, genotypic heterogeneity and comparative pathogenicity of environmental isolates of Papiliotrema laurentii.
Conclusion. Phenotypic methods lacked specificity, but PCR-sequencing of D1/D2 domains correctly identified P. laurentii and sequence comparisons demonstrated the genotypic heterogeneity of the isolates. Both melanized and non-melanized strains at a higher dose caused mortality in immunosuppressed mice and persisted in brain/lung tissue up to 14 days post-infection. PMID: 32945764 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 17, 2020 Category: Microbiology Authors: Asadzadeh M, Ahmad S, Khan Z, Verghese S, Joseph L Tags: J Med Microbiol Source Type: research

Zinc sulfate in combination with a zinc ionophore may improve outcomes in hospitalized COVID-19 patients.
Conclusion. This study provides the first in vivo evidence that zinc sulphate may play a role in therapeutic management for COVID-19. PMID: 32930657 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 14, 2020 Category: Microbiology Authors: Carlucci PM, Ahuja T, Petrilli C, Rajagopalan H, Jones S, Rahimian J Tags: J Med Microbiol Source Type: research

Eucalyptol inhibits biofilm formation of Streptococcus pyogenes and its mediated virulence factors.
Conclusion. The present study suggests that eucalyptol applies its antibiofilm assets by intruding the initial biofilm formation of S. pyogenes. Supplementary studies are needed to understand the mode of action involved in biofilm inhibition. PMID: 32930658 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 14, 2020 Category: Microbiology Authors: Vijayakumar K, Manigandan V, Jeyapragash D, Bharathidasan V, Anandharaj B, Sathya M Tags: J Med Microbiol Source Type: research

Routinely available antimicrobial susceptibility information can be used to increase the efficiency of screening for carbapenemase-producing Enterobacteriaceae.
Conclusion. Enterobacteriaceae isolates with elevated carbapenem MICs that are susceptible to amoxicillin/clavulanic acid or piperacillin/tazobactam are unlikely to be carbapenemase producers. Preselection based on this susceptibility pattern may lead to increased laboratory efficiency and reduction of costs. Whether this is also true for countries with a different distribution of CPE species and types or a higher prevalence of CPE needs to be studied. PMID: 32924918 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 13, 2020 Category: Microbiology Authors: Ong DSY, Altorf-van der Kuil W, Vlek ALM, Schouls LM, Schoffelen AF Tags: J Med Microbiol Source Type: research

An invasive infection with an unusual spaB-possessing Erysipelothrix rhusiopathiae in a human.
Abstract Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals. In humans, in contrast to the cutaneous form called erysipeloid, which is an occupational disease and common in individuals who handle raw meat and fish, invasive systemic infections are unusual. E. rhusiopathiae expresses an immunogenic surface protein, Spa (surface protective antigen), which is involved in virulence. Among the antigenically different Spa proteins (SpaA, B and C), which are mostly associated with serovars, SpaA is by far the most prevalent in E. rhusiopathiae isolates from diseased animals...
Source: Journal of Medical Microbiology - September 13, 2020 Category: Microbiology Authors: Taguchi Y, Shiraiwa K, Ogawa Y, Oi T, Nakamura S, Okumura J, Shimoji Y Tags: J Med Microbiol Source Type: research

Adhesion of Staphylococcus aureus to epithelial cells: an in vitro approach to study interactions within the nasal microbiota.
Conclusion. These findings demonstrate the existence of a negative correlation between S. aureus and other species which inhibits adhesion and could limit nasal colonization. PMID: 32909934 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 9, 2020 Category: Microbiology Authors: Ménard G, Bonnaure-Mallet M, Donnio PY Tags: J Med Microbiol Source Type: research

The TACTIC experience: establishing an international, interdisciplinary network to tackle antimicrobial resistance.
Abstract Antimicrobial resistance (AMR) is a major global health threat that requires an interdisciplinary international approach to address. In response to calls from policymakers and funders alike, a growing number of research networks on AMR have been created with this approach in mind. However, there are many challenges facing researchers in establishing such networks and research projects. In this article, we share our experience of establishing the network 'TACTIC: Tackling AMR Challenges through Translational Interdisciplinary Collaborations'. Although presented with many challenges both scientific and logi...
Source: Journal of Medical Microbiology - September 8, 2020 Category: Microbiology Authors: Al-Hassan L, Roemer-Mahler A, Price J, Islam J, El-Mahallawy H, Higgins PG, Hussein AFA, Roca I, Newport M Tags: J Med Microbiol Source Type: research

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein.
This study evaluated the effect of etomidate against biofilms of Candida spp. and analysed through molecular docking the interaction of this drug with ALS3, an important protein for fungal adhesion. Three fluconazole-resistant fungi were used: Candida albicans, Candida parapsilosis and Candida tropicalis. Growing biofilms were exposed to etomidate at 31.25-500 µg ml-1. Then, an ALS3 adhesive protein from C. albicans was analysed through a molecular mapping technique, composed of a sequence of algorithms to perform molecular mapping simulation based on classic force field theory. Etomidate showed antifun...
Source: Journal of Medical Microbiology - September 3, 2020 Category: Microbiology Authors: Gurgel do Amaral Valente Sá L, da Silva CR, Neto JBA, do Nascimento FBSA, Barroso FDD, da Silva LJ, Cabral VPF, Barbosa AD, Silva J, Marinho ES, de Moraes MO, Rios MEF, Cavalcanti BC, Lima ISP, Júnior HVN Tags: J Med Microbiol Source Type: research

Characterization of φEf-vB1 prophage infecting oral Enterococcus faecalis and enhancing bacterial biofilm formation.
Conclusion. The current findings highlight the role of lysogeny in modification of E. faecalis properties and reveal the potential importance of prophages in E. faecalis biology and pathogenesis. PMID: 32840477 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 24, 2020 Category: Microbiology Authors: Askora A, El-Telbany M, El-Didamony G, Ariny E, Askoura M Tags: J Med Microbiol Source Type: research

Establishment of a Rep' protein antibody detection method to distinguish natural infection with PCV2 from subunit vaccine immunization.
Conclusion. This paper describes an effective iELISA method that can distinguish natural infection with PCV2 (Cap and Rep positive) or inoculation with a whole-virus inactivated vaccine (Cap and Rep positive) from subunit vaccine immunization (Cap-positive, Rep-negative). These comparative assays could be very useful in the control of PCV2 in pig herds. PMID: 32812860 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 18, 2020 Category: Microbiology Authors: Chen Q, Rong J, Li G, Xu B, Wang X, Hu J, Rong M, Li H Tags: J Med Microbiol Source Type: research

Detection and phylogenetic analysis of Human bocavirus in children diagnosed with acute respiratory tract infection.
Abstract Introduction. Human bocavirus (HBoV) is a recently discovered parvovirus; it has been shown to be a common cause of respiratory infections and gastroenteritis in children. Since its identification, HBoV has been detected worldwide in nasopharyngeal swabs, serum and stool samples particularly those obtained from young children suffering from respiratory or gastrointestinal tract infections.Aim. The aim of this work was to determine HBoV prevalence among children with acute respiratory tract infection in Egypt, to detect the most prevalent HBoV genotype and to compare PCR and ELISA as diagnostic techniques ...
Source: Journal of Medical Microbiology - August 18, 2020 Category: Microbiology Authors: Abozahra R, Abdelhamid SM, Khairy K, Baraka K Tags: J Med Microbiol Source Type: research

High frequency of colonization by diverse clones of beta-lactam-resistant Gram-negative bacilli in haemodialysis: different sources of transmission outside the renal unit?
Conclusion. The high colonization by ESBL-GNB in haemodialysis patients shows evidence for the need for stronger surveillance, not only for S. aureus but also for multidrug-resistant bacilli in order to avoid their spread. Additionally, the high genetic diversity suggests other sources of transmission outside the renal unit instead of horizontal transmission between patients. PMID: 32812863 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 18, 2020 Category: Microbiology Authors: Vanegas JM, Salazar-Ospina L, Montoya-Urrego D, Builes J, Roncancio GE, Jiménez JN Tags: J Med Microbiol Source Type: research

Epidemiological and clinical characteristics of coronavirus disease (COVID-19) cases at a screening clinic during the early outbreak period: a single-centre study.
Conclusion. In this single-centre, prospective, descriptive study, fever, cough and shortness of breath were the most common symptoms. Old age (>50 years), chronic underlying comorbidities and travel history may be risk factors. Therefore, we concluded that viral nucleic acid amplification tests (NAAT) played an important role in identifying SARS-CoV-2 infection in a screening clinic, which helped with isolation and cohorting of these patients. PMID: 32783802 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 11, 2020 Category: Microbiology Authors: Khan M, Khan H, Khan S, Nawaz M Tags: J Med Microbiol Source Type: research

Molecular docking, dynamics and free energy analyses of Acinetobacter baumannii OXA class enzymes with carbapenems investigating their hydrolytic mechanisms.
Conclusions. Imipenem and meropenem showed MIC values of 2 and 8 µg ml-1, respectively against OXA-51 in earlier studies, indicating that these are the most effective drugs for treatment of A. baumannii infection. According to our results, OXA-51 is an active enzyme that shows better interactions and is capable of hydrolyzing carbapenems. When correlating the hydrogen-bonding interaction with MIC values, the predicted results are in good agreement and might provide initial insights into performing similar studies related to OXA variants or other antibiotic-enzyme-based studies. PMID: 32773005 [PubM...
Source: Journal of Medical Microbiology - August 9, 2020 Category: Microbiology Authors: Ramachandran B, Jeyakanthan J, Lopes BS Tags: J Med Microbiol Source Type: research

Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA.
Conclusion. With a simplified workflow, The N1 gene Single Tube Optigene LAMP assay (N1-STOP-LAMP) is a powerful, scalable option for specific and rapid detection of SARS-CoV-2 and an additional resource in the diagnostic armamentarium against COVID-19. PMID: 32755529 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 4, 2020 Category: Microbiology Authors: Lee JYH, Best N, McAuley J, Porter JL, Seemann T, Schultz MB, Sait M, Orlando N, Mercoulia K, Ballard SA, Druce J, Tran T, Catton MG, Pryor MJ, Cui HL, Luttick A, McDonald S, Greenhalgh A, Kwong JC, Sherry NL, Graham M, Hoang T, Herisse M, Pidot SJ, Willi Tags: J Med Microbiol Source Type: research

Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens.
Abstract Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (n=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). Mycobacterium tuberculosis was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for ...
Source: Journal of Medical Microbiology - August 4, 2020 Category: Microbiology Authors: Mboneni TA, Eales OO, Mosina NL, Fourie PB Tags: J Med Microbiol Source Type: research

Molecular characterization of respiratory syncytial virus circulating in Tunisia between 2015 and 2018.
Conclusion. These data suggest that RSV-A genotype ON1 and RSV-B genotype BA10, both with duplications in the G gene, were widely circulating in the Central coastal region of Tunisia between 2015 and 2018. PMID: 32755531 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 4, 2020 Category: Microbiology Authors: Jerbi A, Fodha I, Ben Hamida-Rebai M, Ben Hadj Fredj M, Ataoui I, Bennour H, Abroug S, Khlifa M, Mathlouthi J, Mahdhaoui N, Boussetta K, Trabelsi A Tags: J Med Microbiol Source Type: research

The epidemiology and molecular characteristics of linezolid-resistant Staphylococcus capitis in Huashan Hospital, Shanghai.
Conclusions. In this research, cfr-negative strains displayed linezolid MICs of 32 μg ml-1. In comparison, cfr-positive strains exhibited linezolid MICs of 128-512 μg ml-1, indicating that high levels of linezolid resistance appear to be related to the presence of cfr. The outbreak of LZR S. capitis in our hospital needs to be monitored closely. PMID: 32729813 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 29, 2020 Category: Microbiology Authors: Ding L, Li P, Yang Y, Lin D, Xu X Tags: J Med Microbiol Source Type: research

Streptomyces from traditional medicine: sources of new innovations in antibiotic discovery.
Abstract Given the increased reporting of multi-resistant bacteria and the shortage of newly approved medicines, researchers have been looking towards extreme and unusual environments as a new source of antibiotics. Streptomyces currently provides many of the world's clinical antibiotics, so it comes as no surprise that these bacteria have recently been isolated from traditional medicine. Given the wide array of traditional medicines, it is hoped that these discoveries can provide the much sought after core structure diversity that will be required of a new generation of antibiotics. This review discusses the cont...
Source: Journal of Medical Microbiology - July 20, 2020 Category: Microbiology Authors: Quinn GA, Banat AM, Abdelhameed AM, Banat IM Tags: J Med Microbiol Source Type: research

Molecular characterization of a novel bla CTX-M-3-carrying Tn6741 transposon in Morganella morganii isolated from swine.
Conclusion. This is the first time that bla CTX-M-3 has been identified on a chromosome from a M. morganii isolate. Furthermore, the bla CTX-M-3 gene was located with an IS26 element and bla TEM-1 gene on a novel IS26-flanked composite transposon, Tn6741, suggesting that Tn6741 might act as a reservoir for the bla CTX-M-3 and bla TEM-1 genes and may become an important vehicle for their dissemination among M. morganii. PMID: 32692646 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 20, 2020 Category: Microbiology Authors: Luo X, Zhai Y, He D, Cui X, Yang Y, Yuan L, Liu J, Hu G Tags: J Med Microbiol Source Type: research

Improved treatment strategies can result in better outcomes following one-stage exchange surgery for MRSA periprosthetic joint infection.
Conclusion. One-stage exchange surgery using current techniques could improve surgical outcomes with excellent results in the management of MRSA-PJIs. PMID: 32639225 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 7, 2020 Category: Microbiology Authors: Ohlmeier M, Filitarin S, Delgado G, Frings J, Abdelaziz H, Salber J, Frommelt L, Gehrke T, Citak M Tags: J Med Microbiol Source Type: research

Does sub-culturing of positive MRSA blood cultures affect vancomycin MICs?
Conclusion. Our results reveal a trend towards lower MICs after positive blood culture isolates are sub-cultured. Some clinical failures among MRSA infections treated with VAN may result from this phenomenon. PMID: 32639226 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 7, 2020 Category: Microbiology Authors: Kanesaka I, Ito T, Shishido R, Nagashima M, Kanayama Katsuse A, Takahashi H, Fujisaki S, Kobayashi I Tags: J Med Microbiol Source Type: research

The One Health European Joint Programme (OHEJP), 2018-2022: an exemplary One Health initiative.
PMID: 32633710 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 6, 2020 Category: Microbiology Authors: Brown HL, Passey JL, Getino M, Pursley I, Basu P, Horton DL, La Ragione RM Tags: J Med Microbiol Source Type: research

Reversal of heavy metal-induced antibiotic resistance by dandelion root extracts and taraxasterol.
Conclusion. This study suggests that heavy metals induce bacterial antibiotic resistance and dandelion root extracts and taraxasterol could be used to help reverse bacterial resistance to antibiotics. PMID: 32602832 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 29, 2020 Category: Microbiology Authors: Yang K, Zhang Y Tags: J Med Microbiol Source Type: research

Matrix-assisted laser desorption/ionization time-of-flight MS for the accurate identification of Burkholderia cepacia complex and Burkholderia gladioli in the clinical microbiology laboratory.
Conclusion. Currently available MALDI-TOF systems frequently do not accurately identify Bcc bacteria to the species level. PMID: 32597748 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 28, 2020 Category: Microbiology Authors: Wong KSK, Dhaliwal S, Bilawka J, Srigley JA, Champagne S, Romney MG, Tilley P, Sadarangani M, Zlosnik JEA, Chilvers MA Tags: J Med Microbiol Source Type: research

Early life bacterial airway colonization, local immune mediator response and risk of otitis media.
Conclusion. Pathogenic bacterial airway colonization in early life was found to be associated with an increased risk of otitis media, albeit not consistently. These associations were independent of the local immune response to colonization. PMID: 32597749 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 28, 2020 Category: Microbiology Authors: Christensen ED, Thorsen J, Stokholm J, Pedersen TM, Brix S, Krogfelt KA, Schjørring S, Chawes B, Bønnelykke K, Bisgaard H, Rasmussen MA Tags: J Med Microbiol Source Type: research

The rough colony morphotype of Mycobacterium avium exhibits high virulence in human macrophages and mice.
Conclusion. M. avium Rg, which is associated with bacterial aggregation, has the highest virulence among the predominant colony morphotypes. PMID: 32589124 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 25, 2020 Category: Microbiology Authors: Nishimura T, Shimoda M, Tamizu E, Uno S, Uwamino Y, Kashimura S, Yano I, Hasegawa N Tags: J Med Microbiol Source Type: research

In vitro synergistic activity of the sulbactam/avibactam combination against extensively drug-resistant Acinetobacter baumannii.
Conclusions. This study demonstrated that the sulbactam/avibactam combination displayed synergistic activity against A. baumannii isolates. This synergy was observed when both inhibitors were also used as part of the commercially available combinations: ampicillin-sulbactam and ceftazidime-avibactam. PMID: 32584214 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 24, 2020 Category: Microbiology Authors: Rodriguez CH, Brune A, Nastro M, Vay C, Famiglietti A Tags: J Med Microbiol Source Type: research

Prospective multi-center evaluation on risk factors, clinical characteristics and outcomes due to carbapenem resistance in Acinetobacter baumannii complex bacteraemia: experience from the Chinese Antimicrobial Resistance Surveillance of Nosocomial Infections (CARES) Network.
Conclusion. Our findings reveal the epidemiological differences between CRAB BSI and CSAB BSI in a Chinese cohort. Our data suggest that carbapenem resistance has a significant impact on mortality for patients with A. baumannii complex BSI, further strengthening the importance of active prevention and control strategies for the spread of CRAB in Chinese hospitals. PMID: 32584215 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 24, 2020 Category: Microbiology Authors: Liu Y, Wang Q, Zhao C, Chen H, Li H, Wang H, Cares Network OBOT Tags: J Med Microbiol Source Type: research

Comparative evaluation of IS6110 and protein antigen b PCR in cerebrospinal fluid for rapid diagnosis of tuberculous meningitis in children.
Abstract Introduction. Childhood tuberculosis meningitis is a severe form of tuberculosis with high morbidity and mortality. The diagnosis is frequently missed and delayed due to lack of sensitive tests like acid-fast bacilli (AFB) smear and delayed results by culture.Aims. To compare the role of IS6110 and protein antigen b PCR in cerebrospinal fluid (CSF) for rapid diagnosis of tuberculous meningitis (TBM) in children.Methodology. Forty-five cases of TBM and 20 controls were enrolled in this prospective study.Results. The mean ages of cases and controls were 4.2±0.5 years and 4.5±0.7 ...
Source: Journal of Medical Microbiology - June 23, 2020 Category: Microbiology Authors: Agarwal S, Saini AG, Dhawan S, Khadwal A, Sharma K, Singhi P Tags: J Med Microbiol Source Type: research

Whole-genome sequencing analysis of multidrug-resistant Mycobacterium tuberculosis from Java, Indonesia.
Conclusion. Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia. PMID: 32579102 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 23, 2020 Category: Microbiology Authors: Tania T, Sudarmono P, Kusumawati RL, Rukmana A, Pratama WA, Regmi SM, Kaewprasert O, Chaiprasert A, Chongsuvivatwong V, Faksri K Tags: J Med Microbiol Source Type: research

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases.
This study aims to explore the molecular epidemiology and clinical features of HBoV isolates in patients with respiratory diseases.Methodology. Retrospectively, between 2018 and 2020, 5941 respiratory samples from patients with respiratory diseases were screened for respiratory viruses using multiplex real-time PCR. Samples that were positive for HBoV were then subjected to NP1 and VP1/PV2 phylogenetic analysis.Results. HBoV was detected in 1.9 % of the patients, with a peak incidence of infection among children
Source: Journal of Medical Microbiology - June 23, 2020 Category: Microbiology Authors: Madi NM, Al-Adwani A Tags: J Med Microbiol Source Type: research

Shiga toxin-producing Escherichia coli haemolytic uraemic syndrome (STEC-HUS): diagnosis, surveillance and public-health management in England.
PMID: 32552986 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 17, 2020 Category: Microbiology Authors: Jenkins C, Byrne L, Vishram B, Sawyer C, Balasegaram S, Ahyow L, Johnson S Tags: J Med Microbiol Source Type: research

Evaluating the use of a 22-pathogen TaqMan array card for rapid diagnosis of respiratory pathogens in intensive care.
Abstract Introduction. Pneumonia is highly prevalent in intensive care units (ICUs), with high associated mortality. Empirical treatment prioritizes breadth of coverage while awaiting laboratory diagnosis, often at the expense of antimicrobial stewardship. Microarrays use multiple parallel polymerase chain reactions to enable a rapid syndromic approach to laboratory diagnosis.Aim. To evaluate the clinical and laboratory implications of introducing a bespoke 22-pathogen TaqMan Array Card (TAC) for rapid pathogen detection in deep respiratory samples from adult ICUs.Methodology. TAC results from all ICU patients pro...
Source: Journal of Medical Microbiology - June 17, 2020 Category: Microbiology Authors: Jones NK, Conway Morris A, Curran MD, Parmar S, Sule O, Enoch DA, Aliyu SH, Zhang H, Jalal H, Navapurkar V, Murphy ME Tags: J Med Microbiol Source Type: research

Antimicrobial resistance profiles of diarrhoeagenic Escherichia coli isolated from travellers returning to the UK, 2015-2017.
Conclusions. Increasing MDR, along with resistance to the fluroquinolones and the third-generation cephalosporins, in DEC causing travellers' diarrhoea provides further evidence for the need to restrict the use of antimicrobial agents and continuous monitoring. PMID: 32530393 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 11, 2020 Category: Microbiology Authors: Boxall MD, Day MR, Greig DR, Jenkins C Tags: J Med Microbiol Source Type: research

Characterization of Ba813 harbouring Bacillus cereus in patients with haematological malignancy and hospital environments at a medical centre in Japan.
Conclusion. Ba813(+) B. cereus, which are genetically closely related to B. anthracis, were abundant in a haematological ward. Ba813(+) B. cereus with high motility and biofilm formation abilities may spread easily in hospital environments, and could become a hospital-acquired infection. PMID: 32530394 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 11, 2020 Category: Microbiology Authors: Aoyagi T, Kishihara Y, Ogawa M, Ito Y, Tanaka S, Kobayashi R, Tokuda K, Kaku M Tags: J Med Microbiol Source Type: research

Pandemic planning: plotting a course through the coronawars.
Abstract The biological motor behind the current coronavirus pandemic has placed microbiology on a global stage, and given its practitioners a role among the architects of recovery. Planning for a return to normality or the new normal is a complex, multi-agency task for which healthcare scientists may not be prepared. This paper introduces a widely used military planning framework known as the Joint Military Appreciation Process, and outlines how it can be applied to deal with the next phase of the COVID-19 pandemic. Recognition of SARS-CoV-2's critical attributes, targetable vulnerabilities, and its most likely a...
Source: Journal of Medical Microbiology - June 10, 2020 Category: Microbiology Authors: Inglis TJJ Tags: J Med Microbiol Source Type: research

Correlation between cervical HPV DNA detection and HPV16 seroreactivity measured with L1-only and L1+L2 viral capsid antigens.
Conclusion. HPV16 DNA positivity and HPV16 seroreactivity are strongly correlated. L1+L2 VLPs perform better than L1-only VLPs for detecting IgG antibodies to HPV16 in women infected with HPV16 or other Alpha-9 HPV species. This study advances our understanding of humoral immune responses against HPV16 by providing insights about the influence of VLP antigen composition to measure humoral immune response against naturally acquired HPV infection. PMID: 32510304 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 7, 2020 Category: Microbiology Authors: Trevisan A, Candeias JMG, Thomann P, Villa LL, Franco EL, Trottier H, Study Group FTL Tags: J Med Microbiol Source Type: research

Pseudomonas 2019 meeting report.
PMID: 32490791 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 2, 2020 Category: Microbiology Authors: Hardie KR, Mathee K, Schweizer HP, Dietrich LE, Welch M, de Kievit T, Nguyen D, Kivisaar M, Dandekar AA, McDougald D, Winstanley C Tags: J Med Microbiol Source Type: research

Improving the recovery and detection of bloodstream pathogens from blood culture.
Conclusion. The efficiency of bacterial recovery can vary significantly between different methods and thereby can have a critical impact on downstream analysis. The low-speed serum-separation method offered a simple and effective means of recovering common BSI pathogens from blood culture and will be further investigated for use in the rapid detection of bacteraemia and susceptibility testing in clinical practice. PMID: 32490793 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 2, 2020 Category: Microbiology Authors: Falconer K, Hammond R, Gillespie SH Tags: J Med Microbiol Source Type: research

Does concomitant bacteraemia hide the fungi in blood cultures? An in vitro study.
Conclusion. Blood culture bottles including selective fungal media that can allow selective growth of fungi and earlier detection of some species should be preferred in addition to non-selective blood culture bottles, especially in specific patient populations. Further, the use of selective agar plates such as inhibitory mould agar may contribute to the solution of this problem in clinical laboratories. PMID: 32490795 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 2, 2020 Category: Microbiology Authors: Oz Y, Onder S, Alpaslan E, Durmaz G Tags: J Med Microbiol Source Type: research

Efficient differentiation of Nocardia farcinica, Nocardia cyriacigeorgica and Nocardia beijingensis by high-resolution melting analysis using a novel locus.
In conclusion, the developed molecular assay allows simultaneous detection and differentiation of N. farcinica, N. cyriacigeorgica and N. beijingensis with high sensitivity and specificity. PMID: 32478652 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 31, 2020 Category: Microbiology Authors: Xu S, Hou X, Li D, Sun L, Li M, Ji X, Wang X, Li Z Tags: J Med Microbiol Source Type: research

Emerging tick-borne pathogens of public health importance: a mini-review.
Abstract Ticks are the most important vectors of human pathogens, leading to increased public health burdens worldwide. Tick-borne pathogens include viruses (e.g. tick-borne encephalitis and Powassan); bacteria, such as the causative agents of Lyme disease, spotted fever rickettsiosis and human anaplasmosis; and malaria-like protozoan parasites causing babesiosis. Tick-borne diseases are emerging due to the geographical expansion of their tick vectors, especially in the northern hemisphere. Two examples of this phenomenon are Ixodes scapularis and Amblyomma americanum, which have expanded their ranges in the USA i...
Source: Journal of Medical Microbiology - May 31, 2020 Category: Microbiology Authors: Rochlin I, Toledo A Tags: J Med Microbiol Source Type: research

Optimizing a real-time PCR assay for rapid detection of Candida auris in nasal and axillary/groin samples.
Conclusion . The optimized procedure was used to test 1414 patient surveillance samples. The real-time PCR detected all culture-positive samples with 100 % sensitivity and 100 % specificity. PMID: 32478655 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 31, 2020 Category: Microbiology Authors: Malczynski M, Dowllow N, Rezaeian S, Rios J, Dirnberger L, Zembower JA, Zhu A, Qi C Tags: J Med Microbiol Source Type: research

Potential RNA-dependent RNA polymerase inhibitors as prospective therapeutics against SARS-CoV-2.
Conclusion. Quinupristin represents a potential anti-SARS-CoV-2 therapeutic. At present, we have no evidence that this drug is effective against SARS-CoV-2 but expect that the biomedical community will expeditiously follow up on our in silico findings. PMID: 32469301 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 28, 2020 Category: Microbiology Authors: Pokhrel R, Chapagain P, Siltberg-Liberles J Tags: J Med Microbiol Source Type: research