Clinical significance of Pseudomonas aeruginosa 2-alkyl-4-quinolone quorum-sensing signal molecules for long-term outcomes in adults with cystic fibrosis.
Conclusion. Further investigation to confirm the hypothesis that C9-PQS may be associated with increased antibiotic usage for pulmonary exacerbations is warranted as AQ-dependent signalling is a potential future target for anti-virulence therapies. PMID: 31671047 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 31, 2019 Category: Microbiology Authors: Webb K, Fogarty A, Barrett DA, Nash EF, Whitehouse JL, Smyth AR, Stewart I, Knox A, Williams P, Halliday N, Cámara M, Barr HL Tags: J Med Microbiol Source Type: research

Microcolonies: a novel morphological form of pathogenic Mycoplasma spp.
Conclusion. MCs play an unknown role in infection pathology and display prominent antibiotic resistance, making them a challenge for the future studies on Mollicutes. PMID: 31671056 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 31, 2019 Category: Microbiology Authors: Rakovskaya IV, Ermolaeva SA, Levina GA, Barkhatova OI, Mukhachev AY, Andreevskaya SG, Zhukhovitsky VG, Gorina LG, Miller GG, Sysolyatina EV Tags: J Med Microbiol Source Type: research

Same-day antimicrobial susceptibility test using acoustic-enhanced flow cytometry visualized with supervised machine learning.
Conclusions. The combination of machine learning with the FAST method generated same-day AST results and has the potential to aid early antimicrobial treatment decisions, stewardship and detection of resistance. PMID: 31665100 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 30, 2019 Category: Microbiology Authors: Inglis TJJ, Paton TF, Kopczyk MK, Mulroney KT, Carson CF Tags: J Med Microbiol Source Type: research

First Identification and genomic characterization of multidrug-resistant carbapenemase-producing Enterobacteriaceae clinical isolates in Malawi, Africa.
Conclusion. This report strongly suggests a probable existence of highly resistant various types of CPE organisms in Malawi including KPC-2-producing K. pneumoniae ST340/CC258, a known high-risk epidemic lineage. PMID: 31661049 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 29, 2019 Category: Microbiology Authors: Kumwenda GP, Sugawara Y, Abe R, Akeda Y, Kasambara W, Chizani K, Takeuchi D, Sakamoto N, Tomono K, Hamada S Tags: J Med Microbiol Source Type: research

Modified PFGE protocol for improving typeability of DNA degradation susceptible nosocomial Klebsiella pneumoniae.
Conclusion. We have successfully devised a PFGE protocol that enhanced the typeability of nosocomial K. pneumoniae. PMID: 31661052 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 29, 2019 Category: Microbiology Authors: Zakaria AM, Hassuna NA Tags: J Med Microbiol Source Type: research

Emerging multidrug resistance isolates of hospital-acquired bacterial meningitis in a tertiary care centre in North India.
Conclusion. In hospital-acquired bacterial meningitis, multidrug resistance Gram negative bacteria are a huge challenge for the treatment of patients. Hence, antimicrobial stewardship should be followed to counteract with the emerging multidrug resistance isolates. PMID: 31647400 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 24, 2019 Category: Microbiology Authors: Raza MS, Das BK, Goyal V, Lodha R, Chaudhry R, Sood S, Gautam H, Sreenivas V, Nair D, Mohapatra S, Kapil A Tags: J Med Microbiol Source Type: research

Risk factors for the first episode of Acinetobacter baumannii resistant to colistin infection and outcome in critically ill patients.
Abstract Objective. To identify risk factors for the first episode of Acinetobacter baumannii resistant to colistin (ABCR) infection in critically ill patients.Design. Prospective observational study.Setting. Twelve-bed general intensive care unit (ICU) at a University Hospital.Methods. ICU patients who required mechanical ventilation for>48 h during a 36 month period. Clinical and microbiological data were studied; characteristics of patients infected with ABCR were compared with those of critically ill patients who presented infection due to A. baumannii sensitive to colistin (ABCS).Results. Twe...
Source: Journal of Medical Microbiology - October 24, 2019 Category: Microbiology Authors: Mantzarlis K, Makris D, Zakynthinos E Tags: J Med Microbiol Source Type: research

An in vitro experimental study of the effect of fosfomycin in combination with amikacin, ciprofloxacin or meropenem on biofilm formation by multidrug-resistant urinary isolates of Escherichia coli.
Conclusion. The combination of fosfomycin with amikacin and fosfomycin with meropenem yielded a high percentage of synergy alongside an increased capacity to reduce biofilm formation when compared to combination with ciprofloxacin against multidrug-resistant E. coli. Fosfomycin in combination with other classes of antimicrobial agents has potential beneficial effects. PMID: 31647407 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 24, 2019 Category: Microbiology Authors: Sugathan S, Mandal J Tags: J Med Microbiol Source Type: research

Lactobacillus fermentum strains of dairy-product origin adhere to mucin and survive digestive juices.
Conclusion. These preliminary findings suggest a functionality in the two strains of L. fermentum with high colonization potential on GIT mucosal membranes and possible health-promoting effects. This prima facie evidence suggests the need for further studies to test these probiotic candidates as live biotherapeutic agents in vivo. PMID: 31613203 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Aziz K, Haseeb Zaidi A, Fatima HN, Tariq M Tags: J Med Microbiol Source Type: research

A novel multilocus variable-number tandem repeat analysis for Bordetella parapertussis.
Conclusion. Our novel MLVA method has high discriminatory power for genotyping human B. parapertussis. Regarding this organism, this genotyping system is a promising tool for epidemiological surveillance and investigating outbreaks. PMID: 31613204 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Kamachi K, Otsuka N, Fumimoto R, Ozawa K, Yao SM, Chiang CS, Luu LDW, Lan R, Shibayama K, Watanabe M Tags: J Med Microbiol Source Type: research

Rapid and accurate diagnosis of Chlamydia trachomatis in the urogenital tract by a dual-gene multiplex qPCR method.
Conclusion. In this study, a multiplex qPCR assay with high sensitivity and specificity for rapid (≤2.0 h) and accurate diagnosis of C. trachomatis was developed. The qPCR assay has the potential to be used as a routine diagnostic method in clinical microbiology laboratories. PMID: 31613208 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Ma C, Du J, He W, Chen R, Li Y, Dou Y, Yuan X, Zhao L, Gong H, Liu P, Liu H Tags: J Med Microbiol Source Type: research

Myeloid C-type lectin receptors that recognize fungal mannans interact with Pneumocystis organisms and major surface glycoprotein.
In this study, we utilized a library of CLRs that recognize fungal mannans. We used this library to screen against Pneumocystis carinii (Pc) homogenates or purified Pc major surface glycoprotein (Msg) present on Pneumocystis. The results demonstrated that all of the mammalian CLR hFc-fusions tested displayed significant interaction/binding with Pc organisms, and furthermore to isolated Msg. Highest Pc organism and Msg binding activities were with CLR members Mincle, Dectin-2, DC-SIGN and MCL. An immunofluorescence assay with the respective CLR hFc-fusions against whole Pc life forms corroborated these findings. Although so...
Source: Journal of Medical Microbiology - October 14, 2019 Category: Microbiology Authors: Kottom TJ, Hebrink DM, Monteiro JT, Lepenies B, Carmona EM, Wuethrich M, Santo Dias LD, Limper AH Tags: J Med Microbiol Source Type: research

'Medical Mycology' - a new section in the Journal of Medical Microbiology.
PMID: 31609199 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 14, 2019 Category: Microbiology Authors: Chakrabarti A Tags: J Med Microbiol Source Type: research

Laboratory tools for tuberculosis control in a setting with a high burden of HIV/AIDS.
Conclusion. Putative nosocomial transmission of M. tuberculosis was detected, showing that genotyping is a powerful approach for understanding the dynamics of M. tuberculosis transmission, especially in a high-burden TB and HIV landscape. PMID: 31596198 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 9, 2019 Category: Microbiology Authors: Reis AJ, Diniz JLCG, Silva ABS, Silveira J, Basso R, Vieira R, von Groll A, Ramis IB, da Silva PEA Tags: J Med Microbiol Source Type: research

Evaluation of antibody persistence after a four-dose primary hepatitis B vaccination and anamnestic immune response in children under 6 years.
This study was conducted to explore the anti-HBV immune response among children under 6 years old, vaccinated according to the national vaccination schedule, by evaluating the immunological response to primary vaccination and by exploring the anamnestic immune response to a booster dose.Methods. We conducted a cross-sectional prospective study from June 2016 to June 2017 (n=180), based on voluntary participation. Children were recruited from the public pediatric ward sectors in Sahloul University Hospital of Sousse in Central Tunisia. An anti-HB titre was determined based on electro-chemiluminescence micro-particle immunoa...
Source: Journal of Medical Microbiology - October 8, 2019 Category: Microbiology Authors: Tfifha M, Kacem S, Ben Rejeb M, Naija S, Boujaafar N, Abroug S, Trabelsi A Tags: J Med Microbiol Source Type: research

Epidermophyton floccosum: nucleotide sequence analysis and antifungal susceptibility testing of 40 clinical isolates.
Conclusion. Our study showed low intraspecies variation within strains of E. floccosum. Furthermore, terbinafine, posaconazole, itraconazole and voriconazole were shown to be the most potent antifungal drugs against E. floccosum strains. PMID: 31573466 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 1, 2019 Category: Microbiology Authors: Ansari S, Ahmadi B, Norouzi M, Ansari Z, Afsarian MH, Lotfali E, Rezaei-Matehkolaei A Tags: J Med Microbiol Source Type: research

Molecular characterization and immunogenic function of ML1899 (LipG) of Mycobacterium leprae.
Conclusion. These results suggested that ML1899, an esterase could up-regulate the immune responses in favour of macrophages at a low concentration but kills the THP-1 macrophages cells at a higher concentration. PMID: 31553301 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 25, 2019 Category: Microbiology Authors: Rastogi R, Kaur G, Maan P, Bhatnagar A, Narang T, Dogra S, Kaur J Tags: J Med Microbiol Source Type: research

In vitro activities of nine antifungal agents against rare pathogenic fungi.
Conclusion . Our study illustrated in vitro drug susceptibilities of some rare pathogenic fungi, which provide data to guide clinical treatment of fungal infections. PMID: 31553302 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 25, 2019 Category: Microbiology Authors: Sun L, Wan Z, Li R, Yu J Tags: J Med Microbiol Source Type: research

Genomic and phenotypic comparison of environmental and patient-derived isolates of Pseudomonas aeruginosa suggest that antimicrobial resistance is rare within the environment.
Abstract Patient-derived isolates of the opportunistic pathogen Pseudomonas aeruginosa are frequently resistant to antibiotics due to the presence of sequence variants in resistance-associated genes. However, the frequency of antibiotic resistance and of resistance-associated sequence variants in environmental isolates of P. aeruginosa has not been well studied. Antimicrobial susceptibility testing (ciprofloxacin, ceftazidime, meropenem, tobramycin) of environmental (n=50) and cystic fibrosis (n=42) P. aeruginosa isolates was carried out. Following whole genome sequencing of all isolates, 25 resistance-associated ...
Source: Journal of Medical Microbiology - September 25, 2019 Category: Microbiology Authors: Ramsay KA, Wardell SJT, Patrick WM, Brockway B, Reid DW, Winstanley C, Bell SC, Lamont IL Tags: J Med Microbiol Source Type: research

In vitro activity of methylene blue and eosin methylene blue agar on colistin-resistant A. baumannii: an experimental study.
Conclusion. MB may have inhibitory effects against colistin-resistant AB. Secondly, using only EMB agar for subculturing may cause missing of colistin-resistant strains. PMID: 31535963 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 19, 2019 Category: Microbiology Authors: Gazel D, Tatman Otkun M, Akçalı A Tags: J Med Microbiol Source Type: research

Kocuria kristinae: an emerging pathogen in medical practice.
Conclusions. The clinical spectrum of K. kristinae infections has recently widened. The increasing spread of this underestimated bacterium and its resistance to antibiotics represent a new challenge for public health, which requires specific actions to limit it. PMID: 31526454 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 17, 2019 Category: Microbiology Authors: Napolitani M, Troiano G, Bedogni C, Messina G, Nante N Tags: J Med Microbiol Source Type: research

Development and validation of a real-time multiplex PCR assay for the detection of dermatophytes and Fusarium spp.
Conclusion: The multiplex qPCR assays were reliable for the diagnosis of onychomycosis, with shorter turn-around time as compared to culture method. This aids in the planning of treatment strategies to achieve optimal therapeutic outcome. PMID: 31526456 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 17, 2019 Category: Microbiology Authors: Koo SH, Teoh YL, Koh WL, Ochi H, Tan SK, Sim DMF, Jiang B, Tan AL, Tan TY, Lim SPR Tags: J Med Microbiol Source Type: research

Evidence of hypervirulence in Listeria monocytogenes clonal complex 14.
Conclusion. Subgroups of L. monocytogenes possess different virulence phenotypes that may be associated with niche-specificity. While hypervirulent clones have been identified so far in lineage I, our data demonstrate that hypervirulent clones are not restricted to lineage I, as CC14 belongs to lineage II. Identification of subgroups with a higher ability to cause disease may facilitate surveillance and management of listeriosis. PMID: 31524579 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 16, 2019 Category: Microbiology Authors: Cardenas-Alvarez MX, Townsend Ramsett MK, Malekmohammadi S, Bergholz TM Tags: J Med Microbiol Source Type: research

Polymicrobial oral biofilm models: simplifying the complex.
Abstract Over the past century, numerous studies have used oral biofilm models to investigate growth kinetics, biofilm formation, structure and composition, antimicrobial susceptibility and host-pathogen interactions. In vivo animal models provide useful models of some oral diseases; however, these are expensive and carry vast ethical implications. Oral biofilms grown or maintained in vitro offer a useful platform for certain studies and have the advantages of being inexpensive to establish and easy to reproduce and manipulate. In addition, a wide range of variables can be monitored and adjusted to mimic the dynam...
Source: Journal of Medical Microbiology - September 16, 2019 Category: Microbiology Authors: Brown JL, Johnston W, Delaney C, Short B, Butcher MC, Young T, Butcher J, Riggio M, Culshaw S, Ramage G Tags: J Med Microbiol Source Type: research

Epidemiology of Mycoplasma pneumoniae prevalence in Iranian patients: a systematic review and meta-analysis.
Abstract Introduction. Mycoplasma pneumoniae is regarded as the important infectious agent of acute respiratory infections (ARIs) in the world. However, there is little knowledge about the prevalence of M. pneumoniae in Iran. Therefore, the aim of this study was to investigate the prevalence of M. pneumoniae in Iran through a meta-analysis of included studies.Methods. A systematic search was done by using electronic databases from papers that were published by Iranian authors to the end of February 2019. Then, 12 publications, which met our inclusion criteria, were enrolled for data extraction and analysis by usin...
Source: Journal of Medical Microbiology - September 16, 2019 Category: Microbiology Authors: Ranjbar R, Halaji M Tags: J Med Microbiol Source Type: research

Evaluation of inhibitor-combination mCIM for detecting MBL-producing Enterobacterales using three MBL inhibitors.
In this study, we tested the detectability of MBL-producing Enterobacterales against three types of MBL inhibitors (sodium mercaptoacetate, SMA; ethylenediaminetetraacetic acid, EDTA; and dipicolinic acid, DPA) used in combination with a modified carbapenem inactivation method (mCIM). These inhibitor-combination mCIMs were tested against 129 CPE (IMP, 93; NDM, 11; KPC, 13; NMC, 1; OXA-48, 11) and 75 non-CPE. For evaluation of MBL inhibitors, we used two concentrations for each of the three inhibitors: DPA (200 and 300 mg l- 1), EDTA (5 and 10 mM), and SMA (1500 and 3000 mg l- 1). The overall sensitivities of SMA, EDTA and ...
Source: Journal of Medical Microbiology - September 12, 2019 Category: Microbiology Authors: Yamada K, Sasaki M, Imai W, Murakami H, Morita T, Aoki K, Ishii Y, Tateda K Tags: J Med Microbiol Source Type: research

A report on the first outbreak of emm89 group A streptococcus invasive infections in a burns unit in Tunisia.
This report highlights the emergence of the emm89 clone and its capacity to elicit invasive GAS outbreaks. PMID: 31483245 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 4, 2019 Category: Microbiology Authors: Mnif B, Sallem N, Triki Z, Hammami A Tags: J Med Microbiol Source Type: research

Improved quadruplex real-time PCR assay for the diagnosis of diphtheria.
Conclusion. This work describes validation of the improved qPCR quadruplex method and supports its implementation for the biological diagnosis of diphtheria. PMID: 31478826 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 3, 2019 Category: Microbiology Authors: Badell E, Guillot S, Tulliez M, Pascal M, Panunzi LG, Rose S, Litt D, Fry NK, Brisse S Tags: J Med Microbiol Source Type: research

Prosthetic joint infections present diverse and unique microbial communities using combined whole-genome shotgun sequencing and culturing methods.
Conclusions. Variability between and among PJIs showed that most infections were distinct and unique. Comparative analysis of populations revealed PJIs to form clusters that were related to, but separate from, vaginal, skin and gut microbiomes. Fungi and protists were detected by WGSS, but the role of fungi is just beginning to be understood and for protists it is unknown. These micro-organisms and their novel and strain-specific microbial interactions remain to be determined in current clinical tests. PMID: 31460858 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 28, 2019 Category: Microbiology Authors: Weaver AA, Hasan NA, Klaassen M, Karathia H, Colwell RR, Shrout JD Tags: J Med Microbiol Source Type: research

Assessment of the in vitro and in vivo activity of atorvastatin against Candida albicans.
Abstract Aim. The aim of this work was to characterize the response of Candida albicans to atorvastatin, and to assess its in vivo antifungal capability.Methodology. The effect of atorvastatin on the growth and viability of C. albicans was assessed. The ability of the statin to alter cell permeability was quantified by measuring amino acid and protein leakage. The response of C. albicans to atorvastatin was assessed using label-free quantitative proteomics. The in vivo antifungal activity of atorvastatin was assessed using Galleria mellonella larvae infected with C. albicans.Results. Atorvastatin inhibited the gro...
Source: Journal of Medical Microbiology - August 28, 2019 Category: Microbiology Authors: Ajdidi A, Sheehan G, Abu Elteen K, Kavanagh K Tags: J Med Microbiol Source Type: research

Application of Lactobacillus gasseri 63 AM supernatant to Pseudomonas aeruginosa-infected wounds prevents sepsis in murine models of thermal injury and dorsal excision.
Conclusion. These results suggest the potential of LgCS in preventing sepsis from P. aeruginosa infection in severely burned and other immunocompromised patients. PMID: 31460863 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 28, 2019 Category: Microbiology Authors: Lenzmeier TD, Mudaliar NS, Stanbro JA, Watters C, Ahmad A, Simons MP, Ventolini G, Zak JC, Colmer-Hamood JA, Hamood AN Tags: J Med Microbiol Source Type: research

Breakthrough bloodstream infections in critically ill non-neutropenic patients: higher incidence and better survival than non-breakthrough infections.
Conclusion. Breakthrough BSIs were common among critically ill non-neutropenic patients and these patients were associated with better survival because they were de facto receiving appropriate antibiotics. PMID: 31436523 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 23, 2019 Category: Microbiology Authors: Papadimitriou-Olivgeris M, Kolonitsiou F, Solomou A, Karamouzos V, Anastassiou ED, Christofidou M, Spiliopoulou I, Fligou F, Marangos M Tags: J Med Microbiol Source Type: research

Investigating the transient and persistent effects of heat on Clostridium difficile spores.
Abstract Purpose. Clostridium difficile spores are extremely resilient to high temperatures. Sublethal temperatures are associated with the 'reactivation' of dormant spores, and are utilized to maximize C. difficile spore recovery. Spore eradication is of vital importance to the food industry. The current study seeks to elucidate the transient and persisting effects of heating C. difficile spores at various temperatures.Methods. Spores of five C. difficile strains of different ribotypes (001, 015, 020, 027 and 078) were heated at 50, 60 and 70-80 °C for 60 min in phosphate-buffered saline (PBS) a...
Source: Journal of Medical Microbiology - August 20, 2019 Category: Microbiology Authors: Pickering DS, Vernon JJ, Freeman J, Wilcox MH, Chilton CH Tags: J Med Microbiol Source Type: research

From cell culture to cynomolgus macaque: infection models show lineage-specific virulence potential of Coxiella burnetii.
Abstract Coxiella burnetii is an obligate intracellular pathogen that causes the zoonotic disease Q fever in humans, which can occur in either an acute or a chronic form with serious complications. The bacterium has a wide host range, including unicellular organisms, invertebrates, birds and mammals, with livestock representing the most significant reservoir for human infections. Cell culture models have been used to decipher the intracellular lifestyle of C. burnetii, and several infection models, including invertebrates, rodents and non-human primates, are being used to investigate host-pathogen interactions and...
Source: Journal of Medical Microbiology - August 19, 2019 Category: Microbiology Authors: Metters G, Norville IH, Titball RW, Hemsley CM Tags: J Med Microbiol Source Type: research

Intestinal microbiota and colorectal cancer: changes in the intestinal microenvironment and their relation to the disease.
eluzio MDCG Abstract Tools that predict the risk of colorectal cancer are important for early diagnosis, given the high mortality rate for this cancer. The composition of the intestinal microbiota is now considered to be a risk factor for the development of colorectal cancer. This discovery has motivated a growing number of studies to identify the micro-organisms responsible for the onset and/or progression of colorectal cancer. With this in mind, this review discusses the relationship between the composition of the intestinal microbiota and colorectal cancer risk. Prospective and case-control studies indicate tha...
Source: Journal of Medical Microbiology - August 19, 2019 Category: Microbiology Authors: Reis SAD, da Conceição LL, Peluzio MDCG Tags: J Med Microbiol Source Type: research

Prevalence and long-term persistence of beta-haemolytic streptococci throat carriage among children and young adults.
Conclusions. Prevalence was slightly greater among children, but persistent carriage was greater among young adults, with SDSE being the species most associated with persistence. Few sources seemed to disseminate GAS among children, since only two clonal types were found. The volunteers hosted pathogenic streptococci persistently, including macrolide-resistant strains. PMID: 31418669 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2019 Category: Microbiology Authors: O Luiz FB, Alves KB, Barros RR Tags: J Med Microbiol Source Type: research

Urinary microbiota - a potential biomarker and therapeutic target for bladder cancer.
In this study, we collected urine samples from 29 bladder cancer patients and 26 non-cancer patients. To avoid contamination and the impact of antibiotics, urine specimens were collected in a clean manner prior to antibiotic administration. Using the amplicon-based next-generation sequencing approach, the potential determinant bacteria were estimated in a between-group comparison. The results illustrated the differences in microbiota abundance among cancer and non-cancer patients and the overall number of cases carrying these bacteria.Results. We found that the urine samples contained a conserved microbiota with four phyla...
Source: Journal of Medical Microbiology - August 16, 2019 Category: Microbiology Authors: Bi H, Tian Y, Song C, Li J, Liu T, Chen Z, Chen C, Huang Y, Zhang Y Tags: J Med Microbiol Source Type: research

Diphtheria in Belgium: 2010-2017.
ard D Abstract In Western Europe, the incidence of both respiratory and cutaneous diphtheria, caused by toxin-producing Corynebacterium diphtheriae, Corynebacterium ulcerans or Corynebacterium pseudotuberculosis, has been low over the past few decades thanks to the use of an effective vaccine and a high level of vaccination coverage. However, the disease has still not been eradicated and continues to occur in all of Europe. In order to prevent sequelae or a fatal outcome, diphtheria antitoxin (DAT) should be administered to suspected diphtheria patients as soon as possible, but economic factors and issues concerni...
Source: Journal of Medical Microbiology - August 16, 2019 Category: Microbiology Authors: Martini H, Soetens O, Litt D, Fry NK, Detemmerman L, Wybo I, Desombere I, Efstratiou A, Piérard D Tags: J Med Microbiol Source Type: research

Microbial epidemiology and carriage studies for the evaluation of vaccines.
Abstract Respiratory tract infections are responsible for over 2.8 million deaths per year worldwide. Colonization is the first step in the process of microbes occupying the respiratory tract, which may lead to subsequent infection. Carriage, in contrast, is defined as the occupation of microbial species in the respiratory tract. The duration of carriage may be affected by host immunity, the composition and interactions between members of the microbial community, and the characteristics of colonizing bacteria, including physiology associated with being present in a bacterial biofilm. Numerous vaccines have ...
Source: Journal of Medical Microbiology - August 16, 2019 Category: Microbiology Authors: Coughtrie AL, Jefferies JM, Cleary DW, Doncaster CP, Faust SN, Kraaijeveld AR, Moore MV, Mullee MA, Roderick PJ, Webb JS, Yuen HM, Clarke SC Tags: J Med Microbiol Source Type: research

Epidemiological aspects and characterization of the resistance profile of Fusarium spp. in patients with invasive fusariosis.
This study aimed to investigate the epidemiology, clinical features and antifungal susceptibility of Fusarium isolates in patients with invasive fusariosis.Methodology. A total of 27 patients admitted to a referral hospital from January 2008 to June 2017 were evaluated. Antifungal susceptibility testing of isolates was performed by broth microdilution according to the Clinical and Laboratory Standards Institute guidelines.Results. Haematological malignancy was the predominant underlying condition, with an incidence of invasive fusariosis of 14.8 cases per 1000 patients with acute lymphoid leukaemia and 13.1 cases per 1000 ...
Source: Journal of Medical Microbiology - August 16, 2019 Category: Microbiology Authors: Rosa PDD, Ramirez-Castrillon M, Borges R, Aquino V, Meneghello Fuentefria A, Zubaran Goldani L Tags: J Med Microbiol Source Type: research

Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa.
Conclusion. EGCG was able to restore the activity of aztreonam against MDR P. aeruginosa. The data presented support further evaluation of the aztreonam-EGCG combination and highlight its potential for use in clinical medicine. PMID: 31419210 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2019 Category: Microbiology Authors: Betts JW, Hornsey M, Higgins PG, Lucassen K, Wille J, Salguero FJ, Seifert H, La Ragione RM Tags: J Med Microbiol Source Type: research

Streptococcus pneumoniae and Haemophilus influenzae nasopharyngeal molecular detection in children with acute respiratory tract infection in SANADOR Hospital, Romania.
CONCLUSION: A definitive laboratory diagnosis of these micro-organisms can be made for invasive disease, but there are difficulties in establishing the aetiology for mucosal infection. Molecular detection tests could complement culture-based tests by strengthening their surveillance. PMID: 31389781 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 7, 2019 Category: Microbiology Authors: Giucă MC, Cîlcic C, Mihăescu G, Gavrilă A, Dinescu M, Gătej RI Tags: J Med Microbiol Source Type: research

Evaluation of LAMP-based assays for carbapenemase genes.
CONCLUSION: The performance characteristics suggest suitability for diagnostic and surveillance purposes. PMID: 31385779 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 6, 2019 Category: Microbiology Authors: Lahiri S, Venkataraman R, Jagan A, Deshmukh G, Patra S, Reddy V, Sangeetha V, Solanki R, Gupta J, Patel K, De A, Mukhopadhyay C, Dias M, Kanungo R, Mendiratta D, Nawal P, Shastri J, Vemu L, Rangarajan R Tags: J Med Microbiol Source Type: research

Antimicrobial activity of the lupane triterpene 3 β,6β,16β-trihydroxylup-20(29)-ene isolated from Combretum leprosum Mart.
CONCLUSION: In conclusion, the CLF1 compound may be useful in the development of antibacterial drugs against the aforementioned bacteria. PMID: 31385784 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 6, 2019 Category: Microbiology Authors: Cruz BG, Rodrigues Teixeira AM, Silva PTD, Vasconcelos Evaristo FF, de Vaconcelos MA, Holanda Teixeira E, Dos Santos HS, Bandeira PN, Sena-Júnior DM, Barreto VP, Coutinho HDM Tags: J Med Microbiol Source Type: research

Sensitivity of the Candida albicans trehalose-deficient mutants tps1 Δ and tps2Δ to amphotericin B and micafungin.
CONCLUSION: Our data lend weight to the idea of using trehalose biosynthesis as a potential target for antifungal therapy. PMID: 31380734 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 5, 2019 Category: Microbiology Authors: Guirao-Abad JP, Pujante V, Sánchez-Fresneda R, Yagüe G, Argüelles JC Tags: J Med Microbiol Source Type: research

Identification of Haemophilus influenzae serotype e strains missing the fucK gene in clinical isolates from Japan.
CONCLUSIONS: The loss of fucK was frequently observed in clinical isolates of Hie from children. Moreover, fucK-negative Hie may be the cause of IHiD in adult patients. The majority of Hie, including fucK-negative strains, were shown to be clonally related and were ampicillin sensitive. This represents the first report examining fucK losses in encapsulated H. influenzae. PMID: 31368885 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2019 Category: Microbiology Authors: Hoshino T, Takeuchi N, Ohkusu M, Hachisu Y, Hirose S, Fukasawa C, Kubota T, Ishida M, Watanabe H, Oishi K, Ishiwada N Tags: J Med Microbiol Source Type: research

Bacteriocin activity of Lactobacillus brevis and Lactobacillus paracasei ssp. paracasei.
CONCLUSION: Due to their peculiar antimicrobial characteristics, L. paracasei ssp. paracasei LP5 and L. brevis LP9 are suitable for use in the treatment of vaginal disorders, through both oral and transvaginal administration. PMID: 31364964 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 31, 2019 Category: Microbiology Authors: Iseppi R, Messi P, Camellini S, Sabia C Tags: J Med Microbiol Source Type: research

Real-time PCR detection of a 16S rRNA single mutation of Helicobacter pylori isolates associated with reduced susceptibility and resistance to tetracycline in the gastroesophageal mucosa of individual hosts.
, Lehours P Abstract The molecular mechanism of Helicobacter pylori resistance to tetracycline involves mutations in the primary binding site of the ribosome. A resistance or reduced susceptibility to tetracycline could be the result of single, double or triple mutations in the 16S rRNA gene of H. pylori. We investigated if the genotype was correlated to tetracycline resistance as determined phenotypically in vitro for 96 H. pylori isolates in the gastroesophageal mucosa of Venezuelan individual hosts. E-test for antimicrobial susceptibility test and real-time PCR for the detection of 16S rRNA gene mutation...
Source: Journal of Medical Microbiology - July 31, 2019 Category: Microbiology Authors: Contreras M, Benejat L, Mujica H, Peña J, García-Amado MA, Michelangeli F, Lehours P Tags: J Med Microbiol Source Type: research

Synergistic in vitro activity between aztreonam and amoxicillin-clavulanate against Enterobacteriaceae-producing class B and/or class D carbapenemases with or without extended-spectrum β-lactamases.
CONCLUSION: The combination of ATM and AMC could be an attractive unconventional treatment for infections due to carbapenemase- and ESBL-producing Enterobacteriaceae. PMID: 31361213 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 30, 2019 Category: Microbiology Authors: Ract P, Compain F, Robin F, Decre D, Gallah S, Podglajen I Tags: J Med Microbiol Source Type: research

Use of a corneal impression membrane and PCR for the detection of herpes simplex virus type-1.
Abstract PURPOSE: To investigate the use of a corneal impression membrane (CIM) for the detection of herpes simplex virus type 1 (HSV-1) in suspected herpes simplex keratitis (HSK). METHODOLOGY: In the laboratory study, swabs and CIMs made from polytetrafluoroethylene were spiked with different concentrations of HSV-1. DNA was extracted and real-time PCR undertaken using two sets of primers. In the clinical study, consecutive patients presenting with suspected HSK were included. For each patient, samples were collected from corneal lesions with a swab and a CIM in random order. Clinical details were collected...
Source: Journal of Medical Microbiology - July 29, 2019 Category: Microbiology Authors: Brunner M, Somerville T, Corless CE, Myneni J, Rajhbeharrysingh T, Tiew S, Neal T, Kaye SB Tags: J Med Microbiol Source Type: research