Validation of saliva sampling as an alternative to oro-nasopharyngeal swab for detection of SARS-CoV-2 using unextracted rRT-PCR with the Allplex 2019-nCoV assay
Conclusions. Saliva sampling is an acceptable alternative to ONPS for diagnosing SARS-CoV-2 infection in symptomatic individuals displaying symptoms for ≤ 10 days. These results reinforce the need to expand the use of saliva samples, which are self-collected and do not require swabs.PMID:34369860 | DOI:10.1099/jmm.0.001404 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 9, 2021 Category: Microbiology Authors: Marco Andres Bergevin Wesley Freppel Guylaine Robert Georges Ambaraghassi Dany Aubry Olivier Haeck Maude Saint-Jean Alex Carignan Source Type: research

Validation of saliva sampling as an alternative to oro-nasopharyngeal swab for detection of SARS-CoV-2 using unextracted rRT-PCR with the Allplex 2019-nCoV assay
Conclusions. Saliva sampling is an acceptable alternative to ONPS for diagnosing SARS-CoV-2 infection in symptomatic individuals displaying symptoms for ≤ 10 days. These results reinforce the need to expand the use of saliva samples, which are self-collected and do not require swabs.PMID:34369860 | DOI:10.1099/jmm.0.001404 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 9, 2021 Category: Microbiology Authors: Marco Andres Bergevin Wesley Freppel Guylaine Robert Georges Ambaraghassi Dany Aubry Olivier Haeck Maude Saint-Jean Alex Carignan Source Type: research

Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001402.ABSTRACTSeveral studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and s...
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Farah M Shurrab Duaa W Al-Sadeq Fathima Amanullah Salma N Younes Hadeel Al-Jighefee Nadin Younes Soha R Dargham Hadi M Yassine Laith J Abu Raddad Gheyath K Nasrallah Source Type: research

Validation of Pefloxacin for detection of fluoroquinolone (FQ) resistance among < em > Salmonella < /em > Typhi with special reference to GyrB mutations
Conclusion. The pefloxacin disc can be used to detect FQ resistance among S. Typhi. This is the first report of validation of pefloxacin for detection of FQ resistance in S. Typhi mediated by GyrB mutation.PMID:34356003 | DOI:10.1099/jmm.0.001398 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Shakila Banu Inayath Shobha Broor Ruchi Gupta Priti Agarwal Subhradeep Majumder Anupam Kumar Anveshi Rajni Gaind Source Type: research

Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001402.ABSTRACTSeveral studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and s...
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Farah M Shurrab Duaa W Al-Sadeq Fathima Amanullah Salma N Younes Hadeel Al-Jighefee Nadin Younes Soha R Dargham Hadi M Yassine Laith J Abu Raddad Gheyath K Nasrallah Source Type: research

Validation of Pefloxacin for detection of fluoroquinolone (FQ) resistance among < em > Salmonella < /em > Typhi with special reference to GyrB mutations
Conclusion. The pefloxacin disc can be used to detect FQ resistance among S. Typhi. This is the first report of validation of pefloxacin for detection of FQ resistance in S. Typhi mediated by GyrB mutation.PMID:34356003 | DOI:10.1099/jmm.0.001398 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 6, 2021 Category: Microbiology Authors: Shakila Banu Inayath Shobha Broor Ruchi Gupta Priti Agarwal Subhradeep Majumder Anupam Kumar Anveshi Rajni Gaind Source Type: research

Increasingly limited options for the treatment of enteric fever in travellers returning to England, 2014-2019: a cross-sectional analytical study
Conclusion. Third-generation cephalosporins and azithromycin remain appropriate choices for empirical treatment of enteric fever in most returning travellers to the UK from endemic countries, except from Pakistan, where XDR represents a significant risk.PMID:34351258 | DOI:10.1099/jmm.0.001359 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 5, 2021 Category: Microbiology Authors: M Trent Herdman Basel Karo Jayshree Dave Parisha Katwa Joanne Freedman Vivienne Do Nascimento Hilary Kirkbride Marie Anne Chattaway Gauri Godbole Sooria Balasegaram Source Type: research

Increasingly limited options for the treatment of enteric fever in travellers returning to England, 2014-2019: a cross-sectional analytical study
Conclusion. Third-generation cephalosporins and azithromycin remain appropriate choices for empirical treatment of enteric fever in most returning travellers to the UK from endemic countries, except from Pakistan, where XDR represents a significant risk.PMID:34351258 | DOI:10.1099/jmm.0.001359 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 5, 2021 Category: Microbiology Authors: M Trent Herdman Basel Karo Jayshree Dave Parisha Katwa Joanne Freedman Vivienne Do Nascimento Hilary Kirkbride Marie Anne Chattaway Gauri Godbole Sooria Balasegaram Source Type: research

Increased biofilm formation by < em > Staphylococcus aureus < /em > clinical isolates on surfaces covered with plasma proteins
Conclusion. An increase in biofilm production in the presence of plasma proteins highlights the importance of investigating biofilm formation by S. aureus clinical isolates under different conditions since this virulence factor contributes to persistent infections and increased resistance to antimicrobials.PMID:34338626 | DOI:10.1099/jmm.0.001389 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 2, 2021 Category: Microbiology Authors: Lorrayne Cardoso Guimar ães Bruna Marques de Souza Carolina de Oliveira Whitaker Fernanda Abreu Rosana Barreto Rocha Ferreira K átia Regina Netto Dos Santos Source Type: research

Increased biofilm formation by < em > Staphylococcus aureus < /em > clinical isolates on surfaces covered with plasma proteins
Conclusion. An increase in biofilm production in the presence of plasma proteins highlights the importance of investigating biofilm formation by S. aureus clinical isolates under different conditions since this virulence factor contributes to persistent infections and increased resistance to antimicrobials.PMID:34338626 | DOI:10.1099/jmm.0.001389 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 2, 2021 Category: Microbiology Authors: Lorrayne Cardoso Guimar ães Bruna Marques de Souza Carolina de Oliveira Whitaker Fernanda Abreu Rosana Barreto Rocha Ferreira K átia Regina Netto Dos Santos Source Type: research

Increased biofilm formation by < em > Staphylococcus aureus < /em > clinical isolates on surfaces covered with plasma proteins
Conclusion. An increase in biofilm production in the presence of plasma proteins highlights the importance of investigating biofilm formation by S. aureus clinical isolates under different conditions since this virulence factor contributes to persistent infections and increased resistance to antimicrobials.PMID:34338626 | DOI:10.1099/jmm.0.001389 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 2, 2021 Category: Microbiology Authors: Lorrayne Cardoso Guimar ães Bruna Marques de Souza Carolina de Oliveira Whitaker Fernanda Abreu Rosana Barreto Rocha Ferreira K átia Regina Netto Dos Santos Source Type: research

A review of < em > in vitro < /em > attempts to develop the axenic culture of < em > Treponema pallidum < /em > and genomics-based suggestions to achieve this elusive goal
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001388.ABSTRACTTo date, the axenic culture of Treponema pallidum remains a challenge in the field of microbiology despite countless attempts. Here, we conducted a comprehensive bibliographic analysis using several databases and search engines, namely Pubmed, Google scholar, Google, Web of Science and Scopus. Numerous unsuccessful empiric studies have been conducted and evaluated using as criteria dark-field microscopic observation of motile spiral shaped cells in the culture and virulence of the culture through rabbit infectivity. All of these studies failed to induce rab...
Source: Journal of Medical Microbiology - July 30, 2021 Category: Microbiology Authors: Souad Belkacemi Maryam Tidjani Alou Saber Khelaifia Didier Raoult Source Type: research

A review of < em > in vitro < /em > attempts to develop the axenic culture of < em > Treponema pallidum < /em > and genomics-based suggestions to achieve this elusive goal
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001388.ABSTRACTTo date, the axenic culture of Treponema pallidum remains a challenge in the field of microbiology despite countless attempts. Here, we conducted a comprehensive bibliographic analysis using several databases and search engines, namely Pubmed, Google scholar, Google, Web of Science and Scopus. Numerous unsuccessful empiric studies have been conducted and evaluated using as criteria dark-field microscopic observation of motile spiral shaped cells in the culture and virulence of the culture through rabbit infectivity. All of these studies failed to induce rab...
Source: Journal of Medical Microbiology - July 30, 2021 Category: Microbiology Authors: Souad Belkacemi Maryam Tidjani Alou Saber Khelaifia Didier Raoult Source Type: research

A review of < em > in vitro < /em > attempts to develop the axenic culture of < em > Treponema pallidum < /em > and genomics-based suggestions to achieve this elusive goal
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001388.ABSTRACTTo date, the axenic culture of Treponema pallidum remains a challenge in the field of microbiology despite countless attempts. Here, we conducted a comprehensive bibliographic analysis using several databases and search engines, namely Pubmed, Google scholar, Google, Web of Science and Scopus. Numerous unsuccessful empiric studies have been conducted and evaluated using as criteria dark-field microscopic observation of motile spiral shaped cells in the culture and virulence of the culture through rabbit infectivity. All of these studies failed to induce rab...
Source: Journal of Medical Microbiology - July 30, 2021 Category: Microbiology Authors: Souad Belkacemi Maryam Tidjani Alou Saber Khelaifia Didier Raoult Source Type: research

Fluoroquinolone resistance in non-typhoidal < em > Salmonella enterica < /em > isolated from slaughtered pigs in Thailand
Conclusion. We found high fluoroquinolone resistance rates among the NTS isolates from pigs from slaughterhouses. The fluoroquinolone resistance mechanism in NTS was associated with the combination of qnrS and substitutions in gyrA, parC, or both. To prevent the transmission of antimicrobial-resistant NTS between animals and humans, continuous monitoring, surveillance, and regulation of Salmonella in the pork supply chain are pivotal.PMID:34319224 | DOI:10.1099/jmm.0.001386 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 28, 2021 Category: Microbiology Authors: Suleepon Poomchuchit Anusak Kerdsin Peechanika Chopjitt Parichart Boueroy Rujirat Hatrongjit Yukihiro Akeda Kazunori Tomono Suphachai Nuanualsuwan Shigeyuki Hamada Source Type: research

Fluoroquinolone resistance in non-typhoidal < em > Salmonella enterica < /em > isolated from slaughtered pigs in Thailand
Conclusion. We found high fluoroquinolone resistance rates among the NTS isolates from pigs from slaughterhouses. The fluoroquinolone resistance mechanism in NTS was associated with the combination of qnrS and substitutions in gyrA, parC, or both. To prevent the transmission of antimicrobial-resistant NTS between animals and humans, continuous monitoring, surveillance, and regulation of Salmonella in the pork supply chain are pivotal.PMID:34319224 | DOI:10.1099/jmm.0.001386 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 28, 2021 Category: Microbiology Authors: Suleepon Poomchuchit Anusak Kerdsin Peechanika Chopjitt Parichart Boueroy Rujirat Hatrongjit Yukihiro Akeda Kazunori Tomono Suphachai Nuanualsuwan Shigeyuki Hamada Source Type: research

Fluoroquinolone resistance in non-typhoidal < em > Salmonella enterica < /em > isolated from slaughtered pigs in Thailand
Conclusion. We found high fluoroquinolone resistance rates among the NTS isolates from pigs from slaughterhouses. The fluoroquinolone resistance mechanism in NTS was associated with the combination of qnrS and substitutions in gyrA, parC, or both. To prevent the transmission of antimicrobial-resistant NTS between animals and humans, continuous monitoring, surveillance, and regulation of Salmonella in the pork supply chain are pivotal.PMID:34319224 | DOI:10.1099/jmm.0.001386 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 28, 2021 Category: Microbiology Authors: Suleepon Poomchuchit Anusak Kerdsin Peechanika Chopjitt Parichart Boueroy Rujirat Hatrongjit Yukihiro Akeda Kazunori Tomono Suphachai Nuanualsuwan Shigeyuki Hamada Source Type: research

Dental biofilm and its ecological interrelationships in ovine periodontitis
Conclusion. The dental biofilm microbiota associated with ovine periodontitis is dysbiotic and with significant antagonistic interactions, which discriminates healthy animals from diseased animals and highlights the importance of key bacteria, such as Petrimonas, Porphyromonas, Prevotella and Fusobacterium species.PMID:34313584 | DOI:10.1099/jmm.0.001396 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2021 Category: Microbiology Authors: Ana Carolina Borsanelli Fl ávia R F Athayde Sabrina D Agostinho Marcello P Riggio Iveraldo S Dutra Source Type: research

Network pharmacology and molecular docking analysis on mechanisms of Tibetan Hongjingtian ( < em > Rhodiola crenulata < /em > ) in the treatment of COVID-19
Conclusion. R. crenulata might play an anti-inflammatory and immunoregulatory role in the cytokine storm of COVID-19.PMID:34313585 | DOI:10.1099/jmm.0.001374 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2021 Category: Microbiology Authors: Li Wang Yuhe Wang Wei Yang Xue He Shilin Xu Xiaoli Liu Yongjun He Qunying Hu Dongya Yuan Tianbo Jin Source Type: research

The emerging importance of Shiga toxin-producing < em > Escherichia coli < /em > other than serogroup O157 in England
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001375.ABSTRACTIntroduction. Shiga toxin-producing Escherichia coli (STEC) can cause severe disease and large outbreaks. In England, the incidence and clinical significance of STEC serogroups other than O157 (non-O157) is unknown due to a testing bias for detection of STEC O157. Since 2013, the implementation of PCR to detect all STEC serogroups by an increasing number of diagnostic laboratories has led to an increase in the detection of non-O157 STEC.Hypothesis/Gap statement. Due to a bias in testing methodologies to select for STEC serogroup O157 in frontline diagnostic...
Source: Journal of Medical Microbiology - July 26, 2021 Category: Microbiology Authors: Bhavita Vishram Claire Jenkins David R Greig Gauri Godbole Kevin Carroll Sooria Balasegaram Lisa Byrne Source Type: research

Acceptable performance of the Abbott ID NOW among symptomatic individuals with confirmed COVID-19
Conclusion. Results from the ID NOW were reliable, especially when adhering to the manufacturer's recommendations for testing.PMID:34309503 | DOI:10.1099/jmm.0.001372 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 26, 2021 Category: Microbiology Authors: William Stokes Byron M Berenger Takshveer Singh Ifueko Adeghe Angela Schneider Danielle Portnoy Teagan King Brittney Scott Kanti Pabbaraju Sandy Shokoples Anita A Wong Kara Gill LeeAnn Turnbull Jia Hu Graham Tipples Source Type: research

The emerging importance of Shiga toxin-producing < em > Escherichia coli < /em > other than serogroup O157 in England
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001375.ABSTRACTIntroduction. Shiga toxin-producing Escherichia coli (STEC) can cause severe disease and large outbreaks. In England, the incidence and clinical significance of STEC serogroups other than O157 (non-O157) is unknown due to a testing bias for detection of STEC O157. Since 2013, the implementation of PCR to detect all STEC serogroups by an increasing number of diagnostic laboratories has led to an increase in the detection of non-O157 STEC.Hypothesis/Gap statement. Due to a bias in testing methodologies to select for STEC serogroup O157 in frontline diagnostic...
Source: Journal of Medical Microbiology - July 26, 2021 Category: Microbiology Authors: Bhavita Vishram Claire Jenkins David R Greig Gauri Godbole Kevin Carroll Sooria Balasegaram Lisa Byrne Source Type: research

Acceptable performance of the Abbott ID NOW among symptomatic individuals with confirmed COVID-19
Conclusion. Results from the ID NOW were reliable, especially when adhering to the manufacturer's recommendations for testing.PMID:34309503 | DOI:10.1099/jmm.0.001372 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 26, 2021 Category: Microbiology Authors: William Stokes Byron M Berenger Takshveer Singh Ifueko Adeghe Angela Schneider Danielle Portnoy Teagan King Brittney Scott Kanti Pabbaraju Sandy Shokoples Anita A Wong Kara Gill LeeAnn Turnbull Jia Hu Graham Tipples Source Type: research

< em > Shigella < /em > escapes lysosomal degradation through inactivation of Rab31 by IpaH4.5
Conclusion. We identified an unknown function of IpaH4.5 and its potential role in S. flexneri infection.PMID:34296983 | DOI:10.1099/jmm.0.001382 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 23, 2021 Category: Microbiology Authors: Jin Sun Xiaolin Wang Haotian Lin Luming Wan Ji Chen Xiaopan Yang Dongyu Li Yanhong Zhang Xiang He Bin Wang Mingxin Dong Hui Zhong Congwen Wei Source Type: research

Adaptation of < em > gltA < /em > and < em > ssrA < /em > assays for diversity profiling by Illumina sequencing to identify < em > Bartonella henselae, B. clarridgeiae < /em > and < em > B. koehlerae < /em >
Conclusion. The adaptation and multiplexing of existing PCR assays for diversity profiling via NGS is a feasible approach that is superior to traditional Sanger sequencing for Bartonella speciation and resolving mixed Bartonella infections. The adaptation of other PCR primers for Illumina NGS will be useful in future studies where mixed bacterial infections may be present.PMID:34296984 | DOI:10.1099/jmm.0.001400 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 23, 2021 Category: Microbiology Authors: Rosemonde Isabella Power Nichola Elisa Davies Calvani Yaarit Nachum-Biala Harold Salant Shimon Harrus Jan Šlapeta Source Type: research

< em > Shigella < /em > escapes lysosomal degradation through inactivation of Rab31 by IpaH4.5
Conclusion. We identified an unknown function of IpaH4.5 and its potential role in S. flexneri infection.PMID:34296983 | DOI:10.1099/jmm.0.001382 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 23, 2021 Category: Microbiology Authors: Jin Sun Xiaolin Wang Haotian Lin Luming Wan Ji Chen Xiaopan Yang Dongyu Li Yanhong Zhang Xiang He Bin Wang Mingxin Dong Hui Zhong Congwen Wei Source Type: research

Adaptation of < em > gltA < /em > and < em > ssrA < /em > assays for diversity profiling by Illumina sequencing to identify < em > Bartonella henselae, B. clarridgeiae < /em > and < em > B. koehlerae < /em >
Conclusion. The adaptation and multiplexing of existing PCR assays for diversity profiling via NGS is a feasible approach that is superior to traditional Sanger sequencing for Bartonella speciation and resolving mixed Bartonella infections. The adaptation of other PCR primers for Illumina NGS will be useful in future studies where mixed bacterial infections may be present.PMID:34296984 | DOI:10.1099/jmm.0.001400 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 23, 2021 Category: Microbiology Authors: Rosemonde Isabella Power Nichola Elisa Davies Calvani Yaarit Nachum-Biala Harold Salant Shimon Harrus Jan Šlapeta Source Type: research

< em > Shigella < /em > escapes lysosomal degradation through inactivation of Rab31 by IpaH4.5
Conclusion. We identified an unknown function of IpaH4.5 and its potential role in S. flexneri infection.PMID:34296983 | DOI:10.1099/jmm.0.001382 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 23, 2021 Category: Microbiology Authors: Jin Sun Xiaolin Wang Haotian Lin Luming Wan Ji Chen Xiaopan Yang Dongyu Li Yanhong Zhang Xiang He Bin Wang Mingxin Dong Hui Zhong Congwen Wei Source Type: research

Adaptation of < em > gltA < /em > and < em > ssrA < /em > assays for diversity profiling by Illumina sequencing to identify < em > Bartonella henselae, B. clarridgeiae < /em > and < em > B. koehlerae < /em >
Conclusion. The adaptation and multiplexing of existing PCR assays for diversity profiling via NGS is a feasible approach that is superior to traditional Sanger sequencing for Bartonella speciation and resolving mixed Bartonella infections. The adaptation of other PCR primers for Illumina NGS will be useful in future studies where mixed bacterial infections may be present.PMID:34296984 | DOI:10.1099/jmm.0.001400 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 23, 2021 Category: Microbiology Authors: Rosemonde Isabella Power Nichola Elisa Davies Calvani Yaarit Nachum-Biala Harold Salant Shimon Harrus Jan Šlapeta Source Type: research

Hospital transmission of borderline oxacillin-resistant < em > Staphylococcus aureus < /em > evaluated by whole-genome sequencing
Conclusion. WGS and cgMLST effectively characterized and confirmed BORSA clusters among at-risk patients with clinical manifestations ranging from mild skin infections to life-threatening bacteraemia. Clinical awareness and active monitoring are therefore warranted for the timely implementation of infection control measures to prevent BORSA transmission in high-risk patients.PMID:34269673 | DOI:10.1099/jmm.0.001384 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 16, 2021 Category: Microbiology Authors: Maria M Konstantinovski Karin Ellen Veldkamp Adriana P M Lavrijsen Thijs Bosch Margriet E M Kraakman Sam Nooij Eric C J Claas Jairo Gooskens Source Type: research

Hospital transmission of borderline oxacillin-resistant < em > Staphylococcus aureus < /em > evaluated by whole-genome sequencing
Conclusion. WGS and cgMLST effectively characterized and confirmed BORSA clusters among at-risk patients with clinical manifestations ranging from mild skin infections to life-threatening bacteraemia. Clinical awareness and active monitoring are therefore warranted for the timely implementation of infection control measures to prevent BORSA transmission in high-risk patients.PMID:34269673 | DOI:10.1099/jmm.0.001384 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 16, 2021 Category: Microbiology Authors: Maria M Konstantinovski Karin Ellen Veldkamp Adriana P M Lavrijsen Thijs Bosch Margriet E M Kraakman Sam Nooij Eric C J Claas Jairo Gooskens Source Type: research

Hospital transmission of borderline oxacillin-resistant < em > Staphylococcus aureus < /em > evaluated by whole-genome sequencing
Conclusion. WGS and cgMLST effectively characterized and confirmed BORSA clusters among at-risk patients with clinical manifestations ranging from mild skin infections to life-threatening bacteraemia. Clinical awareness and active monitoring are therefore warranted for the timely implementation of infection control measures to prevent BORSA transmission in high-risk patients.PMID:34269673 | DOI:10.1099/jmm.0.001384 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 16, 2021 Category: Microbiology Authors: Maria M Konstantinovski Karin Ellen Veldkamp Adriana P M Lavrijsen Thijs Bosch Margriet E M Kraakman Sam Nooij Eric C J Claas Jairo Gooskens Source Type: research

Hospital transmission of borderline oxacillin-resistant < em > Staphylococcus aureus < /em > evaluated by whole-genome sequencing
Conclusion. WGS and cgMLST effectively characterized and confirmed BORSA clusters among at-risk patients with clinical manifestations ranging from mild skin infections to life-threatening bacteraemia. Clinical awareness and active monitoring are therefore warranted for the timely implementation of infection control measures to prevent BORSA transmission in high-risk patients.PMID:34269673 | DOI:10.1099/jmm.0.001384 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 16, 2021 Category: Microbiology Authors: Maria M Konstantinovski Karin Ellen Veldkamp Adriana P M Lavrijsen Thijs Bosch Margriet E M Kraakman Sam Nooij Eric C J Claas Jairo Gooskens Source Type: research

Hospital transmission of borderline oxacillin-resistant < em > Staphylococcus aureus < /em > evaluated by whole-genome sequencing
Conclusion. WGS and cgMLST effectively characterized and confirmed BORSA clusters among at-risk patients with clinical manifestations ranging from mild skin infections to life-threatening bacteraemia. Clinical awareness and active monitoring are therefore warranted for the timely implementation of infection control measures to prevent BORSA transmission in high-risk patients.PMID:34269673 | DOI:10.1099/jmm.0.001384 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 16, 2021 Category: Microbiology Authors: Maria M Konstantinovski Karin Ellen Veldkamp Adriana P M Lavrijsen Thijs Bosch Margriet E M Kraakman Sam Nooij Eric C J Claas Jairo Gooskens Source Type: research

Hospital transmission of borderline oxacillin-resistant < em > Staphylococcus aureus < /em > evaluated by whole-genome sequencing
Conclusion. WGS and cgMLST effectively characterized and confirmed BORSA clusters among at-risk patients with clinical manifestations ranging from mild skin infections to life-threatening bacteraemia. Clinical awareness and active monitoring are therefore warranted for the timely implementation of infection control measures to prevent BORSA transmission in high-risk patients.PMID:34269673 | DOI:10.1099/jmm.0.001384 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 16, 2021 Category: Microbiology Authors: Maria M Konstantinovski Karin Ellen Veldkamp Adriana P M Lavrijsen Thijs Bosch Margriet E M Kraakman Sam Nooij Eric C J Claas Jairo Gooskens Source Type: research

8-hydroxyquinoline-5-(N-4-chlorophenyl) sulfonamide and fluconazole combination as a preventive strategy for < em > Candida < /em > biofilm in haemodialysis devices
Conclusion. PH151 demonstrated potential as a local treatment for use in a combination therapy approach against Candida biofilm formation on haemodialysis devices.PMID:34259620 | DOI:10.1099/jmm.0.001377 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 14, 2021 Category: Microbiology Authors: Let ícia Fernandes da Rocha Bruna Pippi Ang élica Rocha Joaquim Saulo Fernandes de Andrade Alexandre Meneghello Fuentefria Source Type: research

Serum biomarkers to differentiate Gram-negative, Gram-positive and fungal infection in febrile patients
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001360.ABSTRACTIntroduction. Contamination of specimens and overuse of broad spectrum antibiotics contribute to false positives and false negatives, respectively. Therefore, useful and applicable biomarkers of bacteremia are still required.Hypothesis/Gap Statement. IL-6 can be used as a serum biomarker to discriminate among bacterial infections and fungal infections in febrile patients with a bloodstream infection.Aim. We aimed to evaluate the diagnostic efficiency of neutrophil/lymphocyte ratio (NLR), procalcitonin (PCT) and interleukin-6 (IL-6) in discriminating Gram-ne...
Source: Journal of Medical Microbiology - July 14, 2021 Category: Microbiology Authors: Dongguang Niu Qian Huang Fan Yang Weiliang Tian Chen Li Lian'an Ding Hong-Chun Fang Yunzhao Zhao Source Type: research

8-hydroxyquinoline-5-(N-4-chlorophenyl) sulfonamide and fluconazole combination as a preventive strategy for < em > Candida < /em > biofilm in haemodialysis devices
Conclusion. PH151 demonstrated potential as a local treatment for use in a combination therapy approach against Candida biofilm formation on haemodialysis devices.PMID:34259620 | DOI:10.1099/jmm.0.001377 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 14, 2021 Category: Microbiology Authors: Let ícia Fernandes da Rocha Bruna Pippi Ang élica Rocha Joaquim Saulo Fernandes de Andrade Alexandre Meneghello Fuentefria Source Type: research

Serum biomarkers to differentiate Gram-negative, Gram-positive and fungal infection in febrile patients
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001360.ABSTRACTIntroduction. Contamination of specimens and overuse of broad spectrum antibiotics contribute to false positives and false negatives, respectively. Therefore, useful and applicable biomarkers of bacteremia are still required.Hypothesis/Gap Statement. IL-6 can be used as a serum biomarker to discriminate among bacterial infections and fungal infections in febrile patients with a bloodstream infection.Aim. We aimed to evaluate the diagnostic efficiency of neutrophil/lymphocyte ratio (NLR), procalcitonin (PCT) and interleukin-6 (IL-6) in discriminating Gram-ne...
Source: Journal of Medical Microbiology - July 14, 2021 Category: Microbiology Authors: Dongguang Niu Qian Huang Fan Yang Weiliang Tian Chen Li Lian'an Ding Hong-Chun Fang Yunzhao Zhao Source Type: research

Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria
In this study, the ability of a loop-mediated isothermal amplification (LAMP)-based method (Isoplex CRE-ART) to rapidly detect carbapenemase-encoding genes bla OXA-48-like, bla OXA-23-like, bla OXA-24-like, bla KPC, bla VIM, bla NDM and bla IMP in 231 carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii isolates was investigated. The accuracy of the LAMP test was compared to results of molecular isolate characterization using a Laboratory Developed Test multiplex carbapenemase PCR assay. The LAMP test correctly identified the presence of on-panel carbapenemases with a sensitivity of 99....
Source: Journal of Medical Microbiology - July 12, 2021 Category: Microbiology Authors: Laura Berneking Lucia Asar Anna Both Benjamin Berinson Martin Aepfelbacher Marc L ütgehetmann Holger Rohde Source Type: research

Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria
In this study, the ability of a loop-mediated isothermal amplification (LAMP)-based method (Isoplex CRE-ART) to rapidly detect carbapenemase-encoding genes bla OXA-48-like, bla OXA-23-like, bla OXA-24-like, bla KPC, bla VIM, bla NDM and bla IMP in 231 carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii isolates was investigated. The accuracy of the LAMP test was compared to results of molecular isolate characterization using a Laboratory Developed Test multiplex carbapenemase PCR assay. The LAMP test correctly identified the presence of on-panel carbapenemases with a sensitivity of 99....
Source: Journal of Medical Microbiology - July 12, 2021 Category: Microbiology Authors: Laura Berneking Lucia Asar Anna Both Benjamin Berinson Martin Aepfelbacher Marc L ütgehetmann Holger Rohde Source Type: research

Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria
In this study, the ability of a loop-mediated isothermal amplification (LAMP)-based method (Isoplex CRE-ART) to rapidly detect carbapenemase-encoding genes bla OXA-48-like, bla OXA-23-like, bla OXA-24-like, bla KPC, bla VIM, bla NDM and bla IMP in 231 carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii isolates was investigated. The accuracy of the LAMP test was compared to results of molecular isolate characterization using a Laboratory Developed Test multiplex carbapenemase PCR assay. The LAMP test correctly identified the presence of on-panel carbapenemases with a sensitivity of 99....
Source: Journal of Medical Microbiology - July 12, 2021 Category: Microbiology Authors: Laura Berneking Lucia Asar Anna Both Benjamin Berinson Martin Aepfelbacher Marc L ütgehetmann Holger Rohde Source Type: research

Rapid typing of carbapenem-resistant < em > Acinetobacter baumannii < /em > and < em > Acinetobacter nosocomialis < /em > by multiplex Pan- and OXA-PCR assays
Conclusion. The Pan-PCR and OXA-PCR assays are promising alternative methods for rapid molecular typing of CRABs and CRANs in a routine laboratory setting.PMID:34236300 | DOI:10.1099/jmm.0.001385 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Hsing-Yu Chen Chuan-Chung Chuang Yu-Ching Chou Wei-Jane Hsu I-Chieh Lin None Action Study Group Jun-Ren Sun Source Type: research

A stop-gain mutation in sigma factor SigH (MAB_3543c) may be associated with tigecycline resistance in < em > Mycobacteroides abscessus < /em >
Conclusion. The MAB_3543c mutation may represent a novel determinant of tigecycline resistance in M. abscessus. The findings of this study will hopefully contribute to our knowledge of potential tigecycline resistance mechanisms in M. abscessus, which may lead to better diagnostics and treatment modalities in the future.PMID:34236301 | DOI:10.1099/jmm.0.001378 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Col Lin Lee Hien Fuh Ng Yun Fong Ngeow Zin Thaw Source Type: research

Rapid typing of carbapenem-resistant < em > Acinetobacter baumannii < /em > and < em > Acinetobacter nosocomialis < /em > by multiplex Pan- and OXA-PCR assays
Conclusion. The Pan-PCR and OXA-PCR assays are promising alternative methods for rapid molecular typing of CRABs and CRANs in a routine laboratory setting.PMID:34236300 | DOI:10.1099/jmm.0.001385 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Hsing-Yu Chen Chuan-Chung Chuang Yu-Ching Chou Wei-Jane Hsu I-Chieh Lin None Action Study Group Jun-Ren Sun Source Type: research

A stop-gain mutation in sigma factor SigH (MAB_3543c) may be associated with tigecycline resistance in < em > Mycobacteroides abscessus < /em >
Conclusion. The MAB_3543c mutation may represent a novel determinant of tigecycline resistance in M. abscessus. The findings of this study will hopefully contribute to our knowledge of potential tigecycline resistance mechanisms in M. abscessus, which may lead to better diagnostics and treatment modalities in the future.PMID:34236301 | DOI:10.1099/jmm.0.001378 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Col Lin Lee Hien Fuh Ng Yun Fong Ngeow Zin Thaw Source Type: research

Rapid typing of carbapenem-resistant < em > Acinetobacter baumannii < /em > and < em > Acinetobacter nosocomialis < /em > by multiplex Pan- and OXA-PCR assays
Conclusion. The Pan-PCR and OXA-PCR assays are promising alternative methods for rapid molecular typing of CRABs and CRANs in a routine laboratory setting.PMID:34236300 | DOI:10.1099/jmm.0.001385 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Hsing-Yu Chen Chuan-Chung Chuang Yu-Ching Chou Wei-Jane Hsu I-Chieh Lin None Action Study Group Jun-Ren Sun Source Type: research

A stop-gain mutation in sigma factor SigH (MAB_3543c) may be associated with tigecycline resistance in < em > Mycobacteroides abscessus < /em >
Conclusion. The MAB_3543c mutation may represent a novel determinant of tigecycline resistance in M. abscessus. The findings of this study will hopefully contribute to our knowledge of potential tigecycline resistance mechanisms in M. abscessus, which may lead to better diagnostics and treatment modalities in the future.PMID:34236301 | DOI:10.1099/jmm.0.001378 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Col Lin Lee Hien Fuh Ng Yun Fong Ngeow Zin Thaw Source Type: research

Rapid typing of carbapenem-resistant < em > Acinetobacter baumannii < /em > and < em > Acinetobacter nosocomialis < /em > by multiplex Pan- and OXA-PCR assays
Conclusion. The Pan-PCR and OXA-PCR assays are promising alternative methods for rapid molecular typing of CRABs and CRANs in a routine laboratory setting.PMID:34236300 | DOI:10.1099/jmm.0.001385 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Hsing-Yu Chen Chuan-Chung Chuang Yu-Ching Chou Wei-Jane Hsu I-Chieh Lin None Action Study Group Jun-Ren Sun Source Type: research

A stop-gain mutation in sigma factor SigH (MAB_3543c) may be associated with tigecycline resistance in < em > Mycobacteroides abscessus < /em >
Conclusion. The MAB_3543c mutation may represent a novel determinant of tigecycline resistance in M. abscessus. The findings of this study will hopefully contribute to our knowledge of potential tigecycline resistance mechanisms in M. abscessus, which may lead to better diagnostics and treatment modalities in the future.PMID:34236301 | DOI:10.1099/jmm.0.001378 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2021 Category: Microbiology Authors: Col Lin Lee Hien Fuh Ng Yun Fong Ngeow Zin Thaw Source Type: research