Genomic investigation of clinically significant coagulase-negative staphylococci
Conclusion. Genomic investigation provides insights into the genetic diversity and natural history of colonization and infection. Such information allows the development of new methodologies to identify and compare relatedness and predict antimicrobial resistance. Future clinical studies that employ suitable sampling frames coupled with the application of high-resolution whole-genome sequencing may aid the development of more discriminatory diagnostic approaches to coagulase-staphylococcal infection.PMID:33704043 | DOI:10.1099/jmm.0.001337 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 11, 2021 Category: Microbiology Authors: Kevin Cole Bridget Atkins Martin Llewelyn John Paul Source Type: research

Emergence of a plasmid-borne tigecycline resistance in < em > Klebsiella pneumoniae < /em > in Vietnam
In this study, we identified K. pneumoniae isolates harbouring the tmexCD1-toprJ1 genes outside of China for the first time. Two tigecycline-resistant K. pneumoniae isolates belonging to ST273 by multilocus sequence typing were collected from different patients in a medical institution in Hanoi, Vietnam, in 2015. Whole-genome sequence analysis revealed that these isolates harboured a 288.0 kb tmexCD1-toprJ1-carrying plasmid with IncFIB and IncHI1B replicons. The tmexCD1-toprJ1 gene cluster was surrounded by several mobile gene elements, including IS26, and the plasmids had high sequence identity with that of K. pneumoniae ...
Source: Journal of Medical Microbiology - March 11, 2021 Category: Microbiology Authors: Aki Hirabayashi Van Thi Thu Ha An Van Nguyen Son Thai Nguyen Keigo Shibayama Masato Suzuki Source Type: research

Implication of efflux pumps and < em > ERG11 < /em > genes in resistance of clinical < em > Trichosporon asahii < /em > isolates to fluconazole
J Med Microbiol. 2021 Mar 10. doi: 10.1099/jmm.0.001236. Online ahead of print.ABSTRACTIntroduction. Trichosporon asahii has been recognized as an opportunistic agent having a limited sensitivity to antifungal treatment.Hypothesis/Gap Statement. Molecular mechanisms of azole resistance have been rarely reported for Trichosproron asahii. Similar to other fungi, we hypothesized that both ERG11 gene mutation and efflux pumps genes hyper-expression were implicated.Aim. The current work aimed to study the sensitivity of clinical T. asahii isolates to different antifungal agents and to explore their resistance mechanisms by mole...
Source: Journal of Medical Microbiology - March 10, 2021 Category: Microbiology Authors: S Abbes H Sellami S Neji H Trabelsi F Makni A Ayadi Source Type: research

Implication of efflux pumps and < em > ERG11 < /em > genes in resistance of clinical < em > Trichosporon asahii < /em > isolates to fluconazole
J Med Microbiol. 2021 Mar 10. doi: 10.1099/jmm.0.001236. Online ahead of print.ABSTRACTIntroduction. Trichosporon asahii has been recognized as an opportunistic agent having a limited sensitivity to antifungal treatment.Hypothesis/Gap Statement. Molecular mechanisms of azole resistance have been rarely reported for Trichosproron asahii. Similar to other fungi, we hypothesized that both ERG11 gene mutation and efflux pumps genes hyper-expression were implicated.Aim. The current work aimed to study the sensitivity of clinical T. asahii isolates to different antifungal agents and to explore their resistance mechanisms by mole...
Source: Journal of Medical Microbiology - March 10, 2021 Category: Microbiology Authors: S Abbes H Sellami S Neji H Trabelsi F Makni A Ayadi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Corrigendum: < em > Stenotrophomonas maltophilia < /em > phenotypic and genotypic features through 4-year cystic fibrosis lung colonization
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001312.NO ABSTRACTPMID:33635186 | DOI:10.1099/jmm.0.001312 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 26, 2021 Category: Microbiology Authors: Eliana Alcaraz Daniela Centr ón Gabriela Camicia Mar ía Paula Quiroga Jos é Di Conza Beatriz Passerini de Rossi Source Type: research

Normal breathing releases SARS-CoV-2 into the air
This study tests the release of SARS-CoV-2 RNA into the air during normal breathing, without any sign of possible risk of contagion such as coughing, sneezing or talking. Five patients underwent oropharyngeal, nasopharyngeal and salivary swabs for real-time reverse transcriptase PCR (RT-PCR) detection of SARS-CoV-2 RNA. Direct SARS-CoV-2 release during normal breathing was also investigated by RT-PCR in air samples collected using a microbiological sampler. Viral RNA was detected in air at 1 cm from the mouth of patients whose oropharyngeal, nasopharyngeal and salivary swabs tested positive for SARS-CoV-2 RNA. In contrast,...
Source: Journal of Medical Microbiology - February 25, 2021 Category: Microbiology Authors: Piero Di Carlo Katia Falasca Claudio Ucciferri Bruna Sinjari Eleonora Aruffo Ivana Antonucci Alessandra Di Serafino Arianna Pompilio Verena Damiani Domitilla Mandatori Simone De Fabritiis Beatrice Dufrusine Emily Capone Piero Chiacchiaretta William H Brun Source Type: research

Normal breathing releases SARS-CoV-2 into the air
This study tests the release of SARS-CoV-2 RNA into the air during normal breathing, without any sign of possible risk of contagion such as coughing, sneezing or talking. Five patients underwent oropharyngeal, nasopharyngeal and salivary swabs for real-time reverse transcriptase PCR (RT-PCR) detection of SARS-CoV-2 RNA. Direct SARS-CoV-2 release during normal breathing was also investigated by RT-PCR in air samples collected using a microbiological sampler. Viral RNA was detected in air at 1 cm from the mouth of patients whose oropharyngeal, nasopharyngeal and salivary swabs tested positive for SARS-CoV-2 RNA. In contrast,...
Source: Journal of Medical Microbiology - February 25, 2021 Category: Microbiology Authors: Piero Di Carlo Katia Falasca Claudio Ucciferri Bruna Sinjari Eleonora Aruffo Ivana Antonucci Alessandra Di Serafino Arianna Pompilio Verena Damiani Domitilla Mandatori Simone De Fabritiis Beatrice Dufrusine Emily Capone Piero Chiacchiaretta William H Brun Source Type: research

The simple direct slide method is comparable to indirect Lowenstein Jensen proportion culture for detecting rifampicin resistant tuberculosis
Conclusion. The SDST was found to be a rapid and accurate direct test for the detection of rifampicin resistance among retreatment TB cases in low-income settings.PMID:33625351 | DOI:10.1099/jmm.0.001331 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 24, 2021 Category: Microbiology Authors: Beatrice Achan Benon B Asiimwe Moses L Joloba Mourad Gumusboga Willy Ssengooba Freddie Bwanga Source Type: research

The immunomodulatory activity of secnidazole-nitazoxanide in a murine cryptosporidiosis model
Conclusions. Application of NTZ-SEC combined therapy may be useful in treatment of C. parvum, especially in cases involving immunosuppression.PMID:33625354 | DOI:10.1099/jmm.0.001327 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 24, 2021 Category: Microbiology Authors: Neveen Madbouly Azza El Amir Asmaa Abdel Kader Ibraheem Rabee Alyaa Farid Source Type: research

The simple direct slide method is comparable to indirect Lowenstein Jensen proportion culture for detecting rifampicin resistant tuberculosis
Conclusion. The SDST was found to be a rapid and accurate direct test for the detection of rifampicin resistance among retreatment TB cases in low-income settings.PMID:33625351 | DOI:10.1099/jmm.0.001331 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 24, 2021 Category: Microbiology Authors: Beatrice Achan Benon B Asiimwe Moses L Joloba Mourad Gumusboga Willy Ssengooba Freddie Bwanga Source Type: research

The immunomodulatory activity of secnidazole-nitazoxanide in a murine cryptosporidiosis model
Conclusions. Application of NTZ-SEC combined therapy may be useful in treatment of C. parvum, especially in cases involving immunosuppression.PMID:33625354 | DOI:10.1099/jmm.0.001327 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 24, 2021 Category: Microbiology Authors: Neveen Madbouly Azza El Amir Asmaa Abdel Kader Ibraheem Rabee Alyaa Farid Source Type: research

Detection of < em > parC < /em > gene mutations associated with quinolone resistance in < em > Mycoplasma genitalium < /em > : evaluation of a multiplex real-time PCR assay
Conclusion. MG+parC(beta2) performed with high concordance compared to Sanger sequencing. Such qPCR assays can assist in understanding causes of treatment failure, inform the development of diagnostic assays, and can be applied to surveillance of mutations in populations. Due to an incomplete understanding of the basis for fluoroquinolone resistance, such tests do not appear to be ready for clinical application.PMID:33612146 | DOI:10.1099/jmm.0.001257 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 22, 2021 Category: Microbiology Authors: Kaveesha Bodiyabadu Jennifer Danielewski Suzanne M Garland Dorothy A Machalek Catriona S Bradshaw Joshua Birnie Samantha Ebeyan Marie Lundgren Gerald Murray Source Type: research

Genetic relatedness of < em > Streptococcus dysgalactiae < /em > isolates causing recurrent bacteraemia
Conclusions. Our results show that the paired isolates were of the same clonal origin, which in turn supports carriage between bacteraemia episodes. The phylogenetic analysis indicates that horizontal gene transfer of the emm-gene between some of the SDSE isolates has occurred.PMID:33616518 | DOI:10.1099/jmm.0.001330 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 22, 2021 Category: Microbiology Authors: Erik Senneby Bj örn Hallström Magnus Rasmussen Source Type: research

Genetic relatedness of Streptococcus dysgalactiae isolates causing recurrent bacteraemia.
Conclusions. Our results show that the paired isolates were of the same clonal origin, which in turn supports carriage between bacteraemia episodes. The phylogenetic analysis indicates that horizontal gene transfer of the emm-gene between some of the SDSE isolates has occurred. PMID: 33616518 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 22, 2021 Category: Microbiology Authors: Senneby E, Hallström B, Rasmussen M Tags: J Med Microbiol Source Type: research

Detection of parC gene mutations associated with quinolone resistance in Mycoplasma genitalium: evaluation of a multiplex real-time PCR assay.
Conclusion. MG+parC(beta2) performed with high concordance compared to Sanger sequencing. Such qPCR assays can assist in understanding causes of treatment failure, inform the development of diagnostic assays, and can be applied to surveillance of mutations in populations. Due to an incomplete understanding of the basis for fluoroquinolone resistance, such tests do not appear to be ready for clinical application. PMID: 33612146 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 19, 2021 Category: Microbiology Authors: Bodiyabadu K, Danielewski J, Garland SM, Machalek DA, Bradshaw CS, Birnie J, Ebeyan S, Lundgren M, Murray G Tags: J Med Microbiol Source Type: research

On the emergence, spread and resistance of Candida auris: host, pathogen and environmental tipping points.
Abstract Over a decade ago, a multidrug-resistant nosocomial fungus Candida auris emerged worldwide and has since become a significant challenge for clinicians and microbiologists across the globe. A resilient pathogen, C. auris survives harsh disinfectants, desiccation and high-saline environments. It readily colonizes the inanimate environment, susceptible patients and causes invasive infections that exact a high toll. Prone to misidentification by conventional microbiology techniques, C. auris rapidly acquires multiple genetic determinants that confer multidrug resistance. Whole-genome sequencing has identified...
Source: Journal of Medical Microbiology - February 18, 2021 Category: Microbiology Authors: Chakrabarti A, Sood P Tags: J Med Microbiol Source Type: research

Fidaxomicin has high in vitro activity against Mycobacterium tuberculosis.
This study aimed to evaluate whether the antibiotic fidaxomicin has in vitro activity against Mycobacterium tuberculosis (Mtb). 38 fully drug-sensitive Mtb strains and 34 multidrug-resistant tuberculosis (MDR-TB) strains were tested using the microplate alamar blue assay (MABA) method to determine the minimum inhibitory concentrations (MICs) for fidaxomicin and rifampicin. Fidaxomicin has high in vitro activity against Mtb and is a potential drug to treat Mtb, and MDR-TB infections in particular. PMID: 33593474 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 17, 2021 Category: Microbiology Authors: Sun Q, Wang S, Liao X, Jiang G, Huang H, Li H, Wang G Tags: J Med Microbiol Source Type: research

Loss of the virulence plasmid by Shigella sonnei promotes its interactions with CD207 and CD209 receptors.
Conclusion. This work demonstrated that S. sonnei rough strains - by losing the virulence plasmid - invaded APCs through interactions with CD209 and CD207 receptors. PMID: 33591245 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 16, 2021 Category: Microbiology Authors: Wu BC, Olivia NA, Tembo JM, He YX, Zhang YM, Xue Y, Ye CL, Lv Y, Li WJ, Jiang LY, Huo XX, Sun ZY, Chen ZJ, Qin JC, Li AY, Park CG, Klena JD, Ding HH, Chen T Tags: J Med Microbiol Source Type: research

Molecular characterization of carbapenem-resistant Enterobacteriaceae and emergence of tigecycline non-susceptible strains in the Henan province in China: a multicentrer study.
Conclusion. A high proportion of CRE strains were carbapenemase-positive, and five carbapenem-resistant K. pneumonia isolates were tigecycline non-susceptible, indicating a need for the ongoing surveillance of CRE and effective measures for the prevention of CRE infections. PMID: 33587030 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 15, 2021 Category: Microbiology Authors: Yan WJ, Jing N, Wang SM, Xu JH, Yuan YH, Zhang Q, Li AL, Chen LH, Zhang JF, Ma B, Ma Q, Li Y Tags: J Med Microbiol Source Type: research

Comparison of drug-susceptibility patterns and gene sequences associated with clarithromycin and azithromycin resistance in Mycobacterium abscessus complex isolates and evaluation of the accumulation of intrinsic macrolide resistance.
Abstract Introduction. Mycobacterium abscessus complex (MABC) is an infectious agent associated with macrolide resistance and treatment failure.Hypothesis/Gap Statement. Despite drug-susceptibility testing for MABC isolates including clarithromycin (CAM), long-term treatment with azithromycin (AZM) for MABC disease is recommended.Aim. We compared phenotypic and genotypic resistance to AZM and CAM in clinical isolates and evaluated the accumulation of intrinsic macrolide resistance (AIM) and morphological changes by macrolides exposure.Methodology. Forty-nine isolates were characterized regarding erm(41) sequevars....
Source: Journal of Medical Microbiology - February 11, 2021 Category: Microbiology Authors: Yoshida S, Tsuyuguchi K, Kobayashi T, Inoue Y, Suzuki K Tags: J Med Microbiol Source Type: research

Diazepam's antifungal activity in fluconazole-resistant Candida spp. and biofilm inhibition in C. albicans: evaluation of the relationship with the proteins ALS3 and SAP5.
This study aimed to evaluate the antifungal effect of diazepam on mature C. albicans biofilms in vitro and its action on biofilm in formation, as well as its mechanism of action and interaction with structures related to the adhesion of C. albicans, ALS3 and SAP5. To determine the MIC, the broth microdilution test was used according to protocol M27-A3 (CLSI, 2008). In vitro biofilm formation tests were performed using 96-well plates, followed by molecular-docking protocols to analyse the binding agent interaction with ALS3 and SAP5 targets. The results indicate that diazepam has antimicrobial activity against planktonic ce...
Source: Journal of Medical Microbiology - February 9, 2021 Category: Microbiology Authors: Juvêncio da Silva L, Dias Barroso FD, Vieira LS, Carlos Mota DR, da Silva Firmino BK, Rocha da Silva C, de Farias Cabral VP, Cândido TM, Sá LGDAV, Barbosa da Silva WM, Silva J, Marinho ES, Cavalcanti BC, de Moraes MO, Júnior HVN, de Andrade Neto JB Tags: J Med Microbiol Source Type: research

Tuberculosis cases in a prison in the extreme south of Brazil.
Conclusion. The presence of a Health Unit may have influenced the low numbers of TB/HIV, TB/HBV, TB/HCV, TB/syphilis coinfections and TB cases resistant to antimicrobials. Recent M. tuberculosis transmission can be inferred based on the high percentage of formatting of clusters. This situation stresses the need to improve active and passive detection, the screening of individuals for TB upon entrance into prison for early detection, and the implementation of prophylactic measures to reduce M. tuberculosis transmission. PMID: 33555247 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 8, 2021 Category: Microbiology Authors: Venske Bierhals D, Busatto C, Silveira MDPDR, da Matta Talaier E, Silva ABS, von Groll A, da Silva PEA, Ramis IB, Reis AJ Tags: J Med Microbiol Source Type: research

Surveillance of invasive meningococcal disease in the south of Brazil: considerations of immunization programme.
Abstract Invasive meningococcal disease (IMD) is a major cause of meningitis and septicaemia worldwide. The switches in serogroup predominance contribute to the unpredictable nature of the disease with significant health impacts. The aim of this study was to determine the epidemiological profile of IMD in Rio Grande do Sul, Santa Catarina and Paraná, three states in the south of Brazil. All meningitis cases confirmed by clinical and/or laboratory criteria notified to the national information system for notifiable diseases between 2015 and 2019 were analysed. Proportions of serogroup and incidence by age wer...
Source: Journal of Medical Microbiology - February 5, 2021 Category: Microbiology Authors: Silveira MM, Crespo da Silva PA, Conrad NL, Oliveira TL, Hartwig DD, Trotter C Tags: J Med Microbiol Source Type: research

The prevalence of dermatophytoses in Brazil: a systematic review.
Abstract Dermatophytosis is a common cutaneous mycosis worldwide whose prevalence in Brazil is still unknown. This systematic review has estimated the burden of dermatophytoses from updated literature data reported in the general Brazilian population. We used the following databases: Web of Science, Medline/PubMed, Embase, The Cochrane Library and Scopus for studies published between 2011 and 2020. Original articles with an emphasis on prevalence data for dermatophytosis in the Brazilian population, and diagnosed by culture exam or molecular biology were eligible. We also assessed the methodological quality of the...
Source: Journal of Medical Microbiology - February 3, 2021 Category: Microbiology Authors: de Oliveira Pereira F, Gomes SM, Lima da Silva S, Paula de Castro Teixeira A, Lima IO Tags: J Med Microbiol Source Type: research

Comparative genome analysis of three Group A Streptococcus dysgalactiae subsp. equisimilis strains isolated in Japan.
Conclusion. The comparative genome analysis shows that the genome of GA-SDSE has a distinct arrangement, enabling the differentiation between S. pyogenes, GA-SDSE, and other SDSE strains using our PCR methods. PMID: 33533709 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 3, 2021 Category: Microbiology Authors: Ishihara H, Ogura K, Nguyen VA, Miyohi-Akiyama T, Okamoto S, Takemoto N Tags: J Med Microbiol Source Type: research

Co-infections of two carbapenemase-producing Enterobacter hormaechei clinical strains isolated from the same diabetes individual in China.
Conclusion. This study reveals two multiple drug-resistant E. hormaechei isolates from diabetes patient samples. E. hormaechei carrying two NDM-resistant genes is already a serious threat, where MCR is an important cause of treatment failure in bacterial infections. This study is a reminder not only to prevent infection in patients with diabetes, but also to constantly monitor the epidemic and spread of the drug-resistant gene. PMID: 33528353 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 2, 2021 Category: Microbiology Authors: Ding M, Shi J, Ud Din A, Liu Y, Zhang F, Yan X, Li Q, Bai J, Chen W, Zhou Y Tags: J Med Microbiol Source Type: research

The first UK report of a rare Cryptosporidium hominis genetic variant isolated during a complex Scottish swimming pool outbreak.
This report describes an outbreak of 12 laboratory-confirmed cryptosporidiosis cases identified as part of a Scottish swimming pool investigation, with 9 primary and 3 secondary cases occurring over an 8-week period. Molecular speciation was successful for 11/12 cases, which revealed 10 Cryptosporidium hominis cases and 1 Cryptosporidium parvum case. Of the 10 C. hominis cases, further typing identified 7 as being an unusual sub-type, IbA6G3, which is the first description in the UK of this rare variant. The remaining three C. hominis cases were identified as the common IbA10G2 subtype. Following implementation of control ...
Source: Journal of Medical Microbiology - January 28, 2021 Category: Microbiology Authors: Polubotho P, Denvir L, Connelly L, Anderson E, Alexander CL Tags: J Med Microbiol Source Type: research

Emergence of carbapenem-resistant Klebsiella pneumoniae harbouring bla OXA-48-like genes in China.
Abstract Klebsiella pneumoniae strains carrying OXA-48-like carbapenemases are increasingly prevalent across the globe. There is thus an urgent need to better understand the mechanisms that underpin the dissemination of bla OXA-48-like carbapenemases. To this end, four ertapenem-resistant K. pneumoniae isolates producing OXA-48-like carbapenemases were isolated from two patients. Genome sequencing revealed that one sequence type (ST) 17 isolate carried bla OXA-181, whilst three isolates from a single patient, two ST76 and one ST15, carried bla OXA-232. The 50514 bp bla OXA-181-harbouring plasmid, pOXA-181_Y...
Source: Journal of Medical Microbiology - January 28, 2021 Category: Microbiology Authors: Chen Y, Fang L, Yang Y, Yan R, Fu Y, Shen P, Zhao D, Chen Y, Hua X, Jiang Y, Moran RA, van Schaik W, Yu Y Tags: J Med Microbiol Source Type: research

Rifampin-resistance-associated mutations in the rifampin-resistance-determining region of the rpoB gene of Mycobacterium tuberculosis clinical isolates in Shanghai, PR China.
This study aimed to investigate the relationship between specific rpoB mutations and the MICs of RIF and rifabutin (RFB) against M. tuberculosis.Methodology. Of the 195 clinical isolates, 105 and 90 isolates were randomly selected from isolates resistant to RIF and sensitive to RIF, respectively. The MICs of 12 agents for M. tuberculosis isolates were determined using commercial Sensititre M. tuberculosis MIC plates and the broth microdilution method. Strains were screened for rpoB mutations by DNA extraction, rpoB gene amplification and DNA sequence analysis.Results. One hundred isolates (95.24 %) were found to have mut...
Source: Journal of Medical Microbiology - January 28, 2021 Category: Microbiology Authors: Guo Y, Cao X, Yang J, Wu X, Liu Y, Wan B, Hu L, Wang H, Yu F Tags: J Med Microbiol Source Type: research

Ex vivo potential of a quinoline-derivative nail lacquer as a new alternative for dermatophytic onychomycosis treatment.
Conclusion. Treatment with the quinoline-derivative lacquer showed a significant inhibitory effect on T. rubrum strain in this infection model. Finally, the compound presents high potential for application in a formulation such as nail lacquer as a possible treatment for dermatophytic onychomycosis. PMID: 33502306 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 27, 2021 Category: Microbiology Authors: Machado GDRM, Pippi B, Berlitz S, Diedrich D, Defferrari D, Lopes W, Gnoatto SCB, Kulkamp-Guerreiro IC, Vainstein MH, Jean M, Van de Weghe P, de Andrade SF, Fuentefria AM Tags: J Med Microbiol Source Type: research

Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
Conclusion. We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components. The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo. PMID: 33492206 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 25, 2021 Category: Microbiology Authors: Skovdal SM, Hansen LK, Ivarsen DM, Zeng G, Büttner H, Rohde H, Jørgensen NP, Meyer RL Tags: J Med Microbiol Source Type: research

Emergence of mgrB locus deletion mediating polymyxin resistance in pandemic KPC-producing Klebsiella pneumoniae ST15 lineage.
r Moreira B Abstract Klebsiella pneumoniae causes a diversity of infections in both healthcare and community settings. This pathogen is showing an increased ability to accumulate antimicrobial resistance and virulence genes, making it a public health concern. Here we describe the whole-genome sequence characteristics of an ST15 colistin-resistant K. pneumoniae isolate obtained from a blood culture of a 79-year-old female patient admitted to a university hospital in Brazil. Kp14U04 was resistant to most clinically useful antimicrobial agents, remaining susceptible only to aminoglycosides and fosfomycin. The colisti...
Source: Journal of Medical Microbiology - January 21, 2021 Category: Microbiology Authors: de Araújo Longo LG, Fontana H, Santos de Sousa V, Chilinque Zambão da Silva N, Souto Martins I, Meurer Moreira B Tags: J Med Microbiol Source Type: research

A large food-borne outbreak of campylobacteriosis in kindergartens and primary schools in Pescara, Italy, May-June 2018.
Conclusion. It is plausible that a failure of the pasteurization process contributed to the contamination of the cheese batches. Timely suspension of the catering service and summer closure of the schools prevented further spread. PMID: 33475480 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 21, 2021 Category: Microbiology Authors: Sorgentone S, Busani L, Calistri P, Robuffo G, Bellino S, Acciari V, Ferri M, Graziani C, Antoci S, Lodi F, Alfonsi V, Cammà C, Fazii P, Andrianou X, Cito F, Lombardi G, Centorotola G, D'Amario M, D'Alterio N, Savini V, De Massis F, Pelatti A, Di Domenic Tags: J Med Microbiol Source Type: research

Helicobacter suis and Helicobacter pylori infection in a colony of research macaques: characterization and clinical correlates.
Conclusion. No clear association between the bacterial numbers of Helicobacter spp. and the degree of inflammation was observed. H. suis is prevalent in this colony of Macaca mulatta but its clinical importance remains unclear. This study corroborates many of the findings in earlier studies of H. suis infection in macaques but also identifies at least one animal in which gastritis and endoscopically visible gastric ulcers were strongly associated with H. suis infection. In this study, serology was an inadequate biomarker for endoscopic evaluation in diagnosis of H. suis infection. PMID: 33475481 [PubMed - as supplied ...
Source: Journal of Medical Microbiology - January 21, 2021 Category: Microbiology Authors: Marini RP, Patterson MM, Muthupalani S, Feng Y, Holcombe H, Swennes AG, Ducore R, Whary MM, Shen Z, Fox JG Tags: J Med Microbiol Source Type: research

Phenotypic and genetic characteristics of Streptococcus mutans isolates from site-specific dental plaque in China.
Conclusions. This is the first report of MLST on site-specific dental plaques in a single subject, and indicates that S. mutans isolated from site-specific dental plaque of a single subject showed similar phenotypes as a result of the isolates were closely related. PMID: 33459586 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 18, 2021 Category: Microbiology Authors: Liu S, Li H, Zhang K, Guo Z, Zheng Q, Hu F, Zhang W, Sun Y, Guan JC Tags: J Med Microbiol Source Type: research

Microbiological pattern of prosthetic hip and knee infections: a high-volume, single-centre experience in China.
Conclusions. The prevalence of culture-negative PJIs is still very high. Gram-positive bacteria are still the main type of pathogens that cause PJIs. Attention should be paid to the high incidence of MRS, such as MRSA and MR-CNS, among PJI patients. Empirical antibiotic treatment should cover Gram-positive isolates, especially Staphylococcus. PMID: 33448922 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 15, 2021 Category: Microbiology Authors: Yu Y, Kong Y, Ye J, Wang A, Si W Tags: J Med Microbiol Source Type: research

In vitro synergy of 5-nitrofurans, vancomycin and sodium deoxycholate against Gram-negative pathogens.
Conclusion. This study demonstrates the synergistic effect of 5-nitrofurans, sodium deoxycholate and vancomycin against Gram-negative pathogens and highlights the potential of the combination as a treatment for Gram-negative and Gram-positive infections. PMID: 33448923 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 15, 2021 Category: Microbiology Authors: Olivera C, Le VVH, Davenport C, Rakonjac J Tags: J Med Microbiol Source Type: research

Novel multiplex PCRs for detection of the most prevalent carbapenemase genes in Gram-negative bacteria within Germany.
Conclusion. A rapid and reliable method was developed for detection and differentiation of 12 of the most prevalent carbapenemase groups found in Germany. This method allows for the rapid testing of clinical isolates prior to species identification and does not require prior phenotypical characterization, constituting a rapid and valuable tool in the management of infections in hospitals. PMID: 33448924 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 15, 2021 Category: Microbiology Authors: Cerezales M, Biniossek L, Gerson S, Xanthopoulou K, Wille J, Wohlfarth E, Kaase M, Seifert H, Higgins PG Tags: J Med Microbiol Source Type: research

Citrobacter koseri stimulates dendritic cells to induce IL-33 expression via abundant ATP production.
Conclusion. C. koseri acts as an allergenic bacterium through ATP production, stimulating dendritic cells to induce IL-33 expression, while LPS released from inactivated C. koseri JCM1658 attenuates this allergenicity. PMID: 33439115 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 13, 2021 Category: Microbiology Authors: Kataoka H, Mori T, Into T Tags: J Med Microbiol Source Type: research

Cholera dynamics: lessons from an epidemic.
Abstract Cholera is a severe diarrhoeal disease that spreads rapidly and affects millions of people each year, resulting in tens of thousands of deaths. The disease is caused by Vibrio cholerae O1 and is characterized by watery diarrhoea that can be lethal if not properly treated. Cholera had not been reported in South America from the late 1800s until 1991, when it was introduced in Peru, wreaking havoc in one of the biggest epidemics reported to date. Within a year, the disease had spread to most of the Latin American region, resulting in millions of cases and thousands of deaths in all affected countries. Despi...
Source: Journal of Medical Microbiology - January 8, 2021 Category: Microbiology Authors: Balasubramanian D, Murcia S, Ogbunugafor CB, Gavilan R, Almagro-Moreno S Tags: J Med Microbiol Source Type: research

Sampling challenges in diagnosis of chronic bacterial infections.
en TR Abstract In recent decades there has been an increase in knowledge of the distribution, species diversity and growth patterns of bacteria in human chronic infections. This has challenged standard diagnostic methods, which have undergone a development to both increase the accuracy of testing as well as to decrease the occurrence of contamination. In particular, the introduction of new technologies based on molecular techniques into the clinical diagnostic process has increased detection and identification of infectious pathogens. Sampling is the first step in the diagnostic process, making it crucial for obta...
Source: Journal of Medical Microbiology - January 7, 2021 Category: Microbiology Authors: Jakobsen TH, Xu Y, Bay L, Schønheyder HC, Jakobsen T, Bjarnsholt T, Thomsen TR Tags: J Med Microbiol Source Type: research

2020: the year of living cautiously.
Abstract 2020 was the year when microbiology burst onto the world stage, not just as the science of small living things, but as the prism through which we understood global events. Clinical logic suffered under pressure arising from an urgent need to confirm or exclude severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. This is a generation's Hobbesian moment in which the public concern for safety and security from infection outweighs the pursuit of personal freedom. The strangeness of a world in which a minute particle wields superhuman power has generated its list of unlikely heroes and menda...
Source: Journal of Medical Microbiology - January 6, 2021 Category: Microbiology Authors: Inglis TJJ Tags: J Med Microbiol Source Type: research