Characterization of pelvic and cervical microbiotas from patients with pelvic inflammatory disease.
CONCLUSION: The NGS-based method provides a more informative profile of pelvic microbiota than the culture method. The pelvic and cervical microbiota from the same patient were inconsistent with each other, and thus therapeutic decisions based on cervical microbiota may lead to antimicrobial misuse. PMID: 30113305 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2018 Category: Microbiology Authors: Wang Y, Zhang Y, Zhang Q, Chen H, Feng Y Tags: J Med Microbiol Source Type: research

Icelandic ovine Mycoplasma ovipneumoniae are variable bacteria that induce limited immune responses in vitro and in vivo.
CONCLUSIONS: The moderate immune responses seen in this study indicate that previous exposure to mycoplasmas is a challenge for vaccination of lambs against M. ovipneumoniae. However, an alternative vaccination strategy, e.g. utilizing a recombinant vaccine, may overcome this vaccination hurdle in endemic regions and we suggest a possible vaccine candidate. PMID: 30113306 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2018 Category: Microbiology Authors: Einarsdottir T, Gunnarsson E, Hjartardottir S Tags: J Med Microbiol Source Type: research

Excess body weight and age associated with the carriage of fluoroquinolone and third-generation cephalosporin resistance genes in commensal Escherichia coli from a cohort of urban Vietnamese children.
CONCLUSION: The carriage of genotypic and phenotypic antimicrobial resistance is highly prevalent among E. coli in healthy children in the community in Vietnam. Future investigations on the carriage of antimicrobial resistant organisms in LMICs should focus on the progression of carriage from birth and structure of the microbiome in obesity. PMID: 30113307 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2018 Category: Microbiology Authors: Thi Quynh Nhi L, Thanh Tuyen H, Duc Trung P, Do Hoang Nhu T, Duy PT, Hao CT, Thi Thanh Nhan N, Vi LL, Thi Diem Tuyet H, Thi Thuy Tien T, Van Vinh Chau N, Khanh Lam P, Thwaites G, Baker S Tags: J Med Microbiol Source Type: research

Microbial otitis media: recent advancements in treatment, current challenges and opportunities.
Abstract Otitis media (OM) is a common disease affecting humans, especially paediatric populations. OM refers to inflammation of the middle ear and can be broadly classified into two types, acute and chronic. Bacterial infection is one of the most common causes of OM. Despite the introduction of vaccines, the incidence of OM remains significantly high worldwide. In this mini-review article, we discuss the recent treatment modalities for OM, such as suspension gel, transcutaneous immunization, and intranasal and transtympanic drug delivery, including therapies that are currently undergoing clinical trials. We provi...
Source: Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Mittal R, Parrish JM, Soni M, Mittal J, Mathee K Tags: J Med Microbiol Source Type: research

Genomic reorganization by IS26 in a blaNDM-5-bearing FII plasmid of Klebsiella pneumoniae isolated from a patient in Japan.
Abstract An NDM-5-producing Klebsiella pneumoniae ST147 strain was isolated from a Japanese patient who had not travelled abroad in at least 5 years. Whole-genome sequencing revealed a genomic rearrangement in an FII plasmid harbouring blaNDM-5 due to the replicative transposition of IS26. A hypothetical structure was proposed for its ancestral plasmid, and comparative genomic analysis of the plasmid suggested the dissemination of structurally similar plasmids harbouring blaNDM-5 in Asian and Middle Eastern countries. PMID: 30074471 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Takeuchi D, Akeda Y, Yoshida H, Hagiya H, Yamamoto N, Nishi I, Yoshioka N, Sugawara Y, Sakamoto N, Shanmugakani RK, Deguchi M, Tomono K, Hamada S Tags: J Med Microbiol Source Type: research

Beyond proteostasis: Roles of type I chaperonins in bacterial pathogenesis.
This article provides a thorough reviews of our current understanding of the different mechanisms involving type I chaperonins during bacteria-host interactions, and suggests new areas to be explored and the potential of finding new targets for fighting bacterial infections. PMID: 30074472 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Ghazaei C, Line El Helou M Tags: J Med Microbiol Source Type: research

The endogenous antiseptic N-chlorotaurine irreversibly inactivates Chlamydia pneumoniae and Chlamydia trachomatis.
CONCLUSIONS: This is the first study demonstrating the antimicrobial activity of NCT against Chlamydiae. Clinical implications of these findings have to be investigated in further trials. PMID: 30074473 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Bellmann-Weiler R, Maass V, Arnitz R, Weiss G, Maass M, Nagl M Tags: J Med Microbiol Source Type: research

Genotyping based on thermal denaturation of amplification products identifies species of the Mycobacterium tuberculosis complex.
CONCLUSION: Sensitivity, specificity and positive and negative predictive values of the method are 100 % for the genus Mycobacterium, which resolves MB, MTB and MA genotypes. Species/genotype agreement is 97.7 % for the panel and 92.3 % for the MTC isolates. This method may be advantageously used to identify the most prevalent MTC species in humans. PMID: 30074475 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Domínguez-Zepahua M, Hernández-Arteaga S, López-Revilla R Tags: J Med Microbiol Source Type: research

Mycoplasma pneumoniae DNA detection and specific antibody class response in patients from two tertiary care hospitals in tropical Sri Lanka.
CONCLUSION: The serological evidence for M. pneumoniae infection in Sri Lanka comprised the following prevalences: 17.5 % (17/97), 7.1 % (13/183) and 1.4 % (2/138) in adults with pneumonia, bronchitis or pharyngitis, respectively. M. pneumoniae DNA was in 52.2 % (12/23) of seropositives and 15.4 % (4/26) of seronegatives. IgG was predominant in PCR positives and negatives. PMID: 30074476 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Wijesooriya LI, Kok T, Perera J, Tilakarathne Y, Sunil-Chandra NP Tags: J Med Microbiol Source Type: research

Forensic investigation into the origin of Pseudomonas aeruginosa PA14 - old but not lost.
PMID: 30067168 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2018 Category: Microbiology Authors: Mathee K Tags: J Med Microbiol Source Type: research

Epidemiology of Pseudomonas aeruginosa in agricultural areas.
This study strongly disputes that notion until the modern molecular technology proves otherwise. This paper examines a spectrum of strains occurring in nature, their habitats, dissemination, their relationship to clinical strains, and the environmental conditions that favor their colonization of plants. The isolates were obtained from clinical specimens, plants, soil, and water. The identity of these strains was confirmed using pyocin typing and biochemical assays. The data reveal that agricultural soils, potted ornamental plants, hoses, fountains, and faucets frequently harbored P. aeruginosa. However, it was not commonly...
Source: Journal of Medical Microbiology - August 1, 2018 Category: Microbiology Authors: Schroth MN, Cho JJ, Green SK, Kominos SD, Microbiology Society Publishing Tags: J Med Microbiol Source Type: research

Characterization of the clonal subpopulation Fiocruz L1-130 of Leptospira interrogans in rats and dogs from Brazil.
CONCLUSION: Understanding the circulation of strain Fiocruz L1-130 is important for the implementation of appropriate control measures. Its circulation highlights the need to treat leptospirosis caused by L. interrogans serogroup Icterohaemorrhagiae as a zoonosis that acts in the human-animal-environment interface, as per the One Health approach. PMID: 30059000 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 30, 2018 Category: Microbiology Authors: Jaeger LH, Pestana CP, Carvalho-Costa FA, Medeiros MA, Lilenbaum W Tags: J Med Microbiol Source Type: research

Multicenter assessment of the rapid Unyvero Blood Culture molecular assay.
CONCLUSION: The Unyvero BCU Application provided fast, reliable results, while significantly improving turnaround time in blood culture diagnostics. PMID: 30051799 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Burrack-Lange SC, Personne Y, Huber M, Winkler E, Weile J, Knabbe C, Görig J, Rohde H Tags: J Med Microbiol Source Type: research

Multi-centric validation of an in-house-developed beacon-based PCR diagnostic assay kit for Chlamydia and Neisseria and portable fluorescence detector.
CONCLUSION: Our kit was found to be as sensitive and specific as commercially available kits. Its low cost and ease of use will make it suitable for the routine diagnosis of C. trachomatis and N. gonorrhoeae in the resource-limited settings of developing countries. PMID: 30051801 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Sachdev D, Wasnik K, Patel AL, Sonkar SC, Desai P, Mania-Pramanik J, Kerkar S, Sethi S, Sharma N, Mittal P, Ghope P, Khandhari A, Saluja D Tags: J Med Microbiol Source Type: research

Performance evaluation of five commercial assays in assessing seroprevalence of HEV antibodies among blood donors.
CONCLUSIONS: Due to discrepancies in the performance of various IgG and IgM assays, seroprevalence studies should be based on furher confirmatory testing for decisive conclusions to be reached. PMID: 30051802 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Al-Absi ES, Al-Sadeq DW, Younis MH, Yassine HM, Abdalla OM, Mesleh AG, Hadwan TA, Amimo JO, Thalib L, Nasrallah GK Tags: J Med Microbiol Source Type: research

Mycoplasma amphoriforme vs M. pneumoniae: similarities and differences between patient characteristics in a regional hospital in the Netherlands.
Abstract Mycoplasma amphoriforme is a species closely related to Mycoplasma pneumoniae, thus far with unknown clinical impact. The application of optimized diagnostics, better capable of differentiating between these two micro-organisms, identified a significant patient population positive for M. amphoriforme. The PCR designed by Ling et al. was used on respiratory samples that originally tested positive for M. pneumoniae (n=78), and identified 29 retrospectively as M. amphoriforme. The aim of this study is to describe and compare both groups. The group infected with M. amphoriforme was significantly older and mor...
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: van Schaik ML, Patberg KW, Wallinga JG, Wolfhagen MJHM, Bruijnesteijn van Coppenraet LES Tags: J Med Microbiol Source Type: research

Quantification of major constituents of biofilms in occluded pancreatic stents.
CONCLUSION: The majority of the pancreatic stents grew polymicro-organisms, and those from patients aged>50 years showed significant deposition of protein, which is a key element in biofilm formation. Understanding the constituents of the biofilms in pancreatic stents could be very useful in developing future strategies for the prevention of biofilm formation. PMID: 30051805 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Vaishnavi C, Gupta PK, Sharma M, Kochhar R Tags: J Med Microbiol Source Type: research

Effect of storage time and temperature on the detection of Pseudomonas aeruginosa, Acanthamoeba and Herpes Simplex Virus from corneal impression membranes.
Abstract The effect of storage time and temperature on the recovery of pathogen DNA from polytetrafluorethylene (PTFE) was investigated. PTFE impression membranes were inoculated with Pseudomonas aeruginosa, Herpes Simplex Virus-1 (HSV-1) or Acanthamoeba and stored at -70 °C, -20 °C, +4 °C or +35 °C. PCR was performed on days 0, 1, 2, 3, 7 and months 1, 3 and 10 post-inoculation. We found no reduction in the DNA recovery of any of the studied microorganisms for the first 3 days of storage up to +35 °C. For HSV-1 and P. aeruginosa, storage for 3 m...
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Somerville TF, Corless CE, Neal T, Kaye SB Tags: J Med Microbiol Source Type: research

High rates of Mycobacterium fortuitum isolation in respiratory samples from Iranian patients with suspected tuberculosis: is it clinically important?
CONCLUSION: These results demonstrated the high frequency of M. fortuitum in respiratory samples and that this bacterium causes transient infection or colonization in patients with underlying pulmonary conditions, such as cystic fibrosis and cigarette smoking-induced. Additionally, it appears that infection with M. fortuitum is particularly common and may be important in patients with HIV. PMID: 30052175 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Irandoost M, Zare Ghanbari M, Sakhaee F, Vaziri F, Rahimi Jamnani F, Siadat SD, Fateh A Tags: J Med Microbiol Source Type: research

Fukugiside, a biflavonoid from Garcinia travancorica inhibits biofilm formation of Streptococcus pyogenes and its associated virulence factors.
CONCLUSION: Fukugiside exhibits potent anti-biofilm and anti-virulence activity against different M serotypes of S. pyogenes. It is also non-toxic, which augurs well for its clinical application. PMID: 30052177 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Nandu TG, Subramenium GA, Shiburaj S, Viszwapriya D, Iyer PM, Balamurugan K, Rameshkumar KB, Karutha Pandian S Tags: J Med Microbiol Source Type: research

Time-kill kinetics of cadazolid and comparator antibacterial agents against different ribotypes of Clostridium difficile.
CONCLUSION: The data presented here demonstrate the potent in vitro bactericidal activity of cadazolid against different ribotypes of C. difficile, although on a limited set of strains. PMID: 30052178 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Skinner K, Birchall S, Corbett D, Thommes P, Locher HH Tags: J Med Microbiol Source Type: research

Evaluation of the FilmArray Blood Culture Identification Panel compared to direct MALDI-TOF MS identification for rapid identification of pathogens.
Abstract To improve time to identification of pathogens and detection of resistance genes, we evaluated the BioFire FilmArray Blood Culture Identification Panel (BCID) as compared to: (1) direct MALDI-TOF MS (DM) and (2) standardized culture-based identification (ID) with antibiotic susceptibility testing (AST). BCID gave an accurate identification in 102/112 (91 %) of cases (102/103 for on-panel organisms). DM gave an accurate identification in 91/112 (81 %) of cases, with 13/91 (14 %) requiring repeat testing from the residual pellet. The mean time to an identification result was 2.4 and 2.9 h for B...
Source: Journal of Medical Microbiology - July 20, 2018 Category: Microbiology Authors: Payne M, Champagne S, Lowe C, Leung V, Hinch M, Romney MG Tags: J Med Microbiol Source Type: research

Pyrosequencing: a rapid and effective sequencing method to diagnose drug-resistant tuberculosis.
CONCLUSION: The PSQ assay is a rapid and effective method for detecting drug resistance mutations from Mtb clinical isolates in a short period of time. PMID: 30028665 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 20, 2018 Category: Microbiology Authors: Govindaswamy A, Sakthi D, Pai R, Jeyaseelan L, Michael JS Tags: J Med Microbiol Source Type: research

Development of a species-specific PCR-RFLP targeting rpoD gene fragment for discrimination of Aeromonas species.
CONCLUSION: This method provides an alternative method for laboratory identification, surveillance and epidemiological investigations of clinical and environmental specimens. PMID: 30024365 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 19, 2018 Category: Microbiology Authors: Puah SM, Khor WC, Kee BP, Tan JAMA, Puthucheary SD, Chua KH Tags: J Med Microbiol Source Type: research

Isolation and characterization of Leptospira interrogans from two patients with leptospirosis in Western Province, Sri Lanka.
This study reports the successful isolation of pathogenic Leptospira from two patients in Western Province, Sri Lanka. PMID: 30024367 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 19, 2018 Category: Microbiology Authors: Nisansala GGT, Muthusinghe D, Gunasekara TDCP, Weerasekera MM, Fernando SSN, Ranasinghe KNP, Marasinghe MGCP, Fernando PS, Koizumi N, Gamage CD Tags: J Med Microbiol Source Type: research

A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products.
CONCLUSION: The key factor in the timely conclusion of this investigation was intersectoral collaboration among epidemiologists, microbiologists, veterinarians, statisticians and health and food safety authorities at national, regional and local levels. PMID: 30024370 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 19, 2018 Category: Microbiology Authors: Anna D, Michela S, Giuliana B, Vicdalia Aniela A, Massimo A, Antonino B, Luca B, Patrizia C, Cesare C, Fabrizio C, Dario M, Marco DD, Violeta DM, Giovanni F, Alfonsina F, Stefano F, Antonietta G, Monica G, Caterina G, Fabrizia G, Maurilia M, Cristina M, D Tags: J Med Microbiol Source Type: research

Comparison of the clinical performance of OmniPlex-HPV and GeneFinder HPV for the detection and genotyping of human papillomaviruses in cervical specimens.
CONCLUSIONS: Our results suggest that OmniPlex-HPV and GeneFinder HPV are highly comparable for the detection and genotyping of HPV, but OmniPlex-HPV displays greater accuracy in cases of multiple HPV infection. PMID: 30024373 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Hong G, Park YG, Jin HW, Choi SK, Chi HY, Cho WJ, Lee JH, Han S, Hong SP Tags: J Med Microbiol Source Type: research

The potential of different molecular biology methods in tracking clones of Acinetobacter baumannii in an ICU setting.
CONCLUSION: M13 PCR can be used in routine environmental surveillance for the detection of persistent antibiotic resistant clones in an ICU setting because of its reliability and simplicity. Further studies based on greater sample size, conducted at the multi-centre level, can give us a better understanding of the reliability of the molecular methods that can be used for the detection of persistent clones in the hospital setting. PMID: 30016226 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Ravi NS, Anandan S, Vijayakumar S, Gopi R, Lopes BS, Veeraraghavan B Tags: J Med Microbiol Source Type: research

In vitro activity of β-lactams in combination with avibactam against multidrug-resistant Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Achromobacter xylosoxidans isolates from patients with cystic fibrosis.
In vitro activity of β-lactams in combination with avibactam against multidrug-resistant Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Achromobacter xylosoxidans isolates from patients with cystic fibrosis. J Med Microbiol. 2018 Jul 17;: Authors: Mathy V, Grohs P, Compain F Abstract The in vitro activity of anti-pseudomonal β-lactams in combination with avibactam was evaluated against 54 multidrug-resistant non-fermenting Gram-negative bacilli isolated from cystic fibrosis patients. Avibactam increased and/or restored the antibacterial activities of ceftazidime and aztreonam again...
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Mathy V, Grohs P, Compain F Tags: J Med Microbiol Source Type: research

Pasteurella multocida inactivated with ferric chloride and adjuvanted with bacterial DNA is a potent and efficacious vaccine in Balb/c mice.
CONCLUSION: These studies indicate that bDNA is effective as an immune adjuvant, and along with stimulatory bDNA represent promising new humoral and cellular immune enhancers for vaccination applications. In addition, this vaccine is able to provide long-term protection against infection. PMID: 30016232 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Homayoon M, Tahamtan Y, Kargar M, Hosseini SMH, Akhavan Sepahy A Tags: J Med Microbiol Source Type: research

Comparative genome and evolution analysis of the locus of enterocyte effacement from enteropathogenic Escherichia coli Deng and its transcriptional response to ciprofloxacin.
CONCLUSIONS: The present findings provide an overview of the phylogenetic characteristics of EPEC Deng and its transcriptional response to ciprofloxacin, further suggesting that GrlA may play a clinically important role in EPEC responses to ciprofloxacin. PMID: 29989530 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 10, 2018 Category: Microbiology Authors: Chen Z, Cheng H, Pan W, Zheng J, Li D, Lin F, Yu Z, Deng Q Tags: J Med Microbiol Source Type: research

Accuracy of a commercial multiplex PCR for the diagnosis of bacterial vaginosis.
CONCLUSION: The ATRiDA test is a sensitive method for the detection of BV but, given the high occurrence of asymptomatic vaginal dysbiosis, a positive test outcome should be interpreted together with clinical symptoms. PMID: 29985123 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 9, 2018 Category: Microbiology Authors: van der Veer C, van Houdt R, van Dam A, de Vries H, Bruisten S Tags: J Med Microbiol Source Type: research

Bactibilia in women affected with diseases of the biliary tract and pancreas. A STROBE guidelines-adherent cross-sectional study in Southern Italy.
In this study, we aimed to assess the bile microbiological flora and its potential link with comorbidity in women. METHODOLOGY: We performed a microbiologic investigation on 53 female patients with biliopancreatic diseases who granted consent, and we analysed the data using a MATLAB platform. RESULTS: We found that the most frequent disease associated with bactibilia was pancreas head carcinoma (PHC) (P=0.0015), while the least frequent disease was gall bladder carcinoma (GBC) (P=0.0002). The most common microorganisms were Pseudomonas spp. (P
Source: Journal of Medical Microbiology - July 5, 2018 Category: Microbiology Authors: Serra N, Di Carlo P, Gulotta G, d' Arpa F, Giammanco A, Colomba C, Melfa G, Fasciana T, Sergi C Tags: J Med Microbiol Source Type: research

Hypervirulent Klebsiella pneumoniae, a 5-year study in a French ICU.
CONCLUSION: hvKp represent a potentially underestimated cause of fatal infections in the Western world. PMID: 29972348 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 4, 2018 Category: Microbiology Authors: Rafat C, Messika J, Barnaud G, Dufour N, Magdoud F, Billard-Pomarès T, Gaudry S, Dreyfuss D, Branger C, Decré D, Ricard JD Tags: J Med Microbiol Source Type: research

Liver involvement is associated with blood culture positivity and high agglutination titre in patients with brucellosis in Turkey.
Abstract We investigated the incidence of and risk factors for liver involvement (LI) in patients with brucellosis in Turkey. Diagnosis was established by blood cultures (BCs) or compatible clinical presentation accompanied by serological evidence. Patients received either a combination of doxycycline plus rifampin for 6 weeks or doxycycline for 6 weeks plus streptomycin intramuscularly for the first 2 weeks. Liver function tests, including those for alanine (ALT) and aspartate aminotransferase (AST) levels, were monitored before and at the end of treatment. One hundred and ninety-five patient...
Source: Journal of Medical Microbiology - July 4, 2018 Category: Microbiology Authors: Sahinturk H, Baran B, Sisman G, Altun R Tags: J Med Microbiol Source Type: research

A clinical isolate of Escherichia coli co-harbouring mcr-1 and blaNDM-5 in Japan.
This study describes the emergence of a colistin- and carbapenem-resistant clinical isolate of Escherichia coli harbouring mcr-1 and blaNDM-5 genes, located on 90 and 150 Kb plasmids, respectively. The isolate belonged to ST132. This is the first report of a clinical isolate in Japan co-harbouring mcr-1 and blaNDM-5. PMID: 29972350 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 4, 2018 Category: Microbiology Authors: Uchida H, Tada T, Sugahara Y, Kato A, Miyairi I, Kirikae T Tags: J Med Microbiol Source Type: research

Novel multiplex PCR assay for identification and subtyping of enteroinvasive Escherichia coli and differentiation from Shigella based on target genes selected by comparative genomics.
CONCLUSION: This new highly specific assay can assist in the identification of EIEC in ipaH PCR-positive samples and augment the public health laboratory surveillance of EIEC and shigellosis. PMID: 29969087 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 3, 2018 Category: Microbiology Authors: Dhakal R, Wang Q, Lan R, Howard P, Sintchenko V Tags: J Med Microbiol Source Type: research

Distribution of genes encoding virulence factors and multilocus variable-number tandem-repeat analysis (MLVA) of entero-aggregative Escherichia coli (EAEC) isolated in Iran from patients with diarrhoea.
CONCLUSION: This study showed that EAEC is a heterogeneous group of E. coli possessing a broad range of virulence factors. There was no notable association between MLVA patterns and virulence profiles. PMID: 29969089 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 3, 2018 Category: Microbiology Authors: Hosseini-Nave H, Samareh Salavati M, Lashkari M, Ravari MA, Hashemizadeh Z, Seyedi Marghaki F, Mohseni F Tags: J Med Microbiol Source Type: research

ASC acts in a caspase-1-independent manner to worsen acute pneumonia caused by Pseudomonas aeruginosa.
CONCLUSIONS: Collectively, these results are consistent with a model whereby the T3SS apparatus of P. aeruginosa activates the caspase-1-dependent inflammasome and caspase-3/7 through an ASC-dependent mechanism. This activation may have implications for the outcomes of P. aeruginosa infections. PMID: 29957172 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 29, 2018 Category: Microbiology Authors: Hughes AJ, Knoten CA, Morris AR, Hauser AR Tags: J Med Microbiol Source Type: research

Antimicrobial resistance profiles of Shigella dysenteriae isolated from travellers returning to the UK, 2004-2017.
Abstract PURPOSE: Antimicrobial resistance (AMR) profiles of 754 strains of Shigella dysenteriae isolated between 2004 and 2017 from UK travellers reporting symptoms of gastrointestinal (GI) disease were reviewed to look for evidence of emerging AMR associated with travellers' diarrhoea. METHODOLOGY: A travel history was provided for 72.7 % (548/754) of cases, of which 90.9 % (498/548) reported travel outside the UK within 7 days of onset of symptoms, and 9.1 % (50/498) reported no travel in that time frame. During the course of this study, whole genome sequencing (WGS) was implemented for GI dis...
Source: Journal of Medical Microbiology - June 29, 2018 Category: Microbiology Authors: Terry LM, Barker CR, Day MR, Greig DR, Dallman TJ, Jenkins C Tags: J Med Microbiol Source Type: research

Corrigendum: Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era.
usílek M, Medvecký M, Papagiannitsis CC PMID: 29957176 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 29, 2018 Category: Microbiology Authors: Žemličková H, Mališová L, Španělová P, Jakubů V, Kozáková J, Musílek M, Medvecký M, Papagiannitsis CC Tags: J Med Microbiol Source Type: research

Identification of recombinant coxsackievirus A6 variants in hand, foot and mouth disease in Nanjing, China, 2013.
CONCLUSION: The emerging recombinant CVA6 variants belong to a novel RF-J group which was most likely formed by at least two recombination events. Continued monitoring on the geographical distribution of various CVA6 RFs is essential. PMID: 29947601 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 27, 2018 Category: Microbiology Authors: Qiao M, Yong W, Wang X, Li W, Zhang Z, He M, Shi L, Wang Y, Xie G, Ding J Tags: J Med Microbiol Source Type: research

Bactericidal efficacy of molybdenum oxide nanoparticles against antimicrobial-resistant pathogens.
usa M Abstract Multidrug-resistant bacteria pose a major threat to effective antibiotics and alternatives to fight multidrug-resistant pathogens are needed. We synthetized molybdenum oxide (MoO3) nanoparticles (NP) and determined their antibacterial activity against 39 isolates: (i) eight Staphylococcus aureus, including representatives of methicillin-resistant S. aureus epidemic clones; (ii) six enterococci, including vancomycin-resistant isolates; and (iii) 25 Gram-negative isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterobacter cloacae), including extende...
Source: Journal of Medical Microbiology - June 25, 2018 Category: Microbiology Authors: Lopes E, Piçarra S, Almeida PL, de Lencastre H, Aires-de-Sousa M Tags: J Med Microbiol Source Type: research

The importance of cross-disciplinary research to combat antimicrobial resistance: introducing a new pop-up journal, X-AMR.
PMID: 29932392 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 22, 2018 Category: Microbiology Authors: Knight G, Lambert H, Feil E, Holmes M, Lindsay J Tags: J Med Microbiol Source Type: research

Ecology and diversity in upper respiratory tract microbial population structures from a cross-sectional community swabbing study.
CONCLUSION: The application of epidemiological methods and ecological theory to respiratory tract samples has yielded novel insights into the factors that drive microbial community composition. PMID: 29927372 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Coughtrie AL, Morris DE, Anderson R, Begum N, Cleary DW, Faust SN, Jefferies JM, Kraaijeveld AR, Moore MV, Mullee MA, Roderick PJ, Tuck A, Whittaker RN, Yuen HM, Doncaster CP, Clarke SC Tags: J Med Microbiol Source Type: research

Epidemic spread of OXA-48 beta-lactamase in Croatia.
CONCLUSIONS: The OXA-48-positive organisms found in this study showed wide variability in antibiotic susceptibility, β-lactamase content and PFGE banding patterns. This study revealed a switch from the predominance of VIM-1 in 2012-2013 to that of OXA-48 in the 2015 to 2017. PMID: 29927373 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Bedenić B, Slade M, Starčević LŽ, Sardelić S, Vranić-Ladavac M, Benčić A, Zujić Atalić V, Bogdan M, Bubonja-Šonje M, Tomić-Paradžik M, Tot T, Lukić-Grlić A, Drenjančević D, Varda-Brkić D, Bandić-Pavlović D, Mihaljević S, Zarfel G, Gu Tags: J Med Microbiol Source Type: research

Genetic diversity of pneumococcal surface protein A (PspA) in paediatric isolates of non-conjugate vaccine serotypes in Japan.
CONCLUSION: The present study revealed the predominance of PspA families 1 and 2 in NVTs, and the presence of family 3 in serotype 37. PMID: 29927374 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Kawaguchiya M, Urushibara N, Aung MS, Morimoto S, Ito M, Kudo K, Kobayashi N Tags: J Med Microbiol Source Type: research

Dissemination of newly emerged polymyxin B sensitive Vibrio cholerae O1 containing Haitian-like genetic traits in different parts of India.
CONCLUSION: Polymyxin B-sensitive strains have begun to spread throughout India and may lead to the revision of the biotype classification. The dissemination of these new variant strains needs to be carefully monitored in different endemic populations through active holistic surveillance to understand their clinical and epidemiological consequences. PMID: 29927375 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Samanta P, Saha RN, Chowdhury G, Naha A, Sarkar S, Dutta S, Nandy RK, Okamoto K, Mukhopadhyay AK Tags: J Med Microbiol Source Type: research

Sexually transmitted rectal infections in a cohort of 'men having sex with men'.
CONCLUSIONS: Sexually transmitted rectal infections are frequent and often asymptomatic among MSM. LGV prevalence is high in our country and there is increasing evidence of symptomless cases. PMID: 29927376 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Foschi C, Gaspari V, Sgubbi P, Salvo M, D'Antuono A, Marangoni A Tags: J Med Microbiol Source Type: research

Streptococcus and Prevotella are associated with the prognosis of oesophageal squamous cell carcinoma.
CONCLUSION: Combined Streptococcus and Prevotella abundance is regarded as an independent species prognostic biomarker in ESCC patients. PMID: 29923815 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 20, 2018 Category: Microbiology Authors: Liu Y, Lin Z, Lin Y, Chen Y, Peng XE, He F, Liu S, Yan S, Huang L, Lu W, Xiang Z, Hu Z Tags: J Med Microbiol Source Type: research