A simple PCR assay for the identification of the novel < em > Streptococcus pneumoniae < /em > serotype 7D
J Med Microbiol. 2023 Aug;72(8). doi: 10.1099/jmm.0.001739.ABSTRACTThe identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.PMID:37552048 | DOI:10.1099/jmm.0.001739 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Karsten Maruhn Andreas Itzek Matthias Imoehl Mark van der Linden Source Type: research
A modified Kirby-Bauer disc diffusion (mKB) method for accurately testing tigecycline susceptibility: a nation-wide multicenter comparative study
Conclusion. The mKB method can test bacterial susceptibility to tigecycline more accurately than sKB. For the tigecycline-intermediate or -resistant strains by sKB method, BMD or mKB method should be used to verify the results and report reliable tigecycline susceptibility results.PMID:37552058 | DOI:10.1099/jmm.0.001671 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Dandan Yin Yan Guo Renru Han Yang Yang Demei Zhu Fupin Hu Source Type: research
A simple PCR assay for the identification of the novel < em > Streptococcus pneumoniae < /em > serotype 7D
J Med Microbiol. 2023 Aug;72(8). doi: 10.1099/jmm.0.001739.ABSTRACTThe identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.PMID:37552048 | DOI:10.1099/jmm.0.001739 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Karsten Maruhn Andreas Itzek Matthias Imoehl Mark van der Linden Source Type: research
A modified Kirby-Bauer disc diffusion (mKB) method for accurately testing tigecycline susceptibility: a nation-wide multicenter comparative study
Conclusion. The mKB method can test bacterial susceptibility to tigecycline more accurately than sKB. For the tigecycline-intermediate or -resistant strains by sKB method, BMD or mKB method should be used to verify the results and report reliable tigecycline susceptibility results.PMID:37552058 | DOI:10.1099/jmm.0.001671 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Dandan Yin Yan Guo Renru Han Yang Yang Demei Zhu Fupin Hu Source Type: research
A simple PCR assay for the identification of the novel < em > Streptococcus pneumoniae < /em > serotype 7D
J Med Microbiol. 2023 Aug;72(8). doi: 10.1099/jmm.0.001739.ABSTRACTThe identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.PMID:37552048 | DOI:10.1099/jmm.0.001739 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Karsten Maruhn Andreas Itzek Matthias Imoehl Mark van der Linden Source Type: research
A modified Kirby-Bauer disc diffusion (mKB) method for accurately testing tigecycline susceptibility: a nation-wide multicenter comparative study
Conclusion. The mKB method can test bacterial susceptibility to tigecycline more accurately than sKB. For the tigecycline-intermediate or -resistant strains by sKB method, BMD or mKB method should be used to verify the results and report reliable tigecycline susceptibility results.PMID:37552058 | DOI:10.1099/jmm.0.001671 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Dandan Yin Yan Guo Renru Han Yang Yang Demei Zhu Fupin Hu Source Type: research
A simple PCR assay for the identification of the novel < em > Streptococcus pneumoniae < /em > serotype 7D
J Med Microbiol. 2023 Aug;72(8). doi: 10.1099/jmm.0.001739.ABSTRACTThe identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.PMID:37552048 | DOI:10.1099/jmm.0.001739 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Karsten Maruhn Andreas Itzek Matthias Imoehl Mark van der Linden Source Type: research
A modified Kirby-Bauer disc diffusion (mKB) method for accurately testing tigecycline susceptibility: a nation-wide multicenter comparative study
Conclusion. The mKB method can test bacterial susceptibility to tigecycline more accurately than sKB. For the tigecycline-intermediate or -resistant strains by sKB method, BMD or mKB method should be used to verify the results and report reliable tigecycline susceptibility results.PMID:37552058 | DOI:10.1099/jmm.0.001671 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Dandan Yin Yan Guo Renru Han Yang Yang Demei Zhu Fupin Hu Source Type: research
A simple PCR assay for the identification of the novel < em > Streptococcus pneumoniae < /em > serotype 7D
J Med Microbiol. 2023 Aug;72(8). doi: 10.1099/jmm.0.001739.ABSTRACTThe identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.PMID:37552048 | DOI:10.1099/jmm.0.001739 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Karsten Maruhn Andreas Itzek Matthias Imoehl Mark van der Linden Source Type: research
A modified Kirby-Bauer disc diffusion (mKB) method for accurately testing tigecycline susceptibility: a nation-wide multicenter comparative study
Conclusion. The mKB method can test bacterial susceptibility to tigecycline more accurately than sKB. For the tigecycline-intermediate or -resistant strains by sKB method, BMD or mKB method should be used to verify the results and report reliable tigecycline susceptibility results.PMID:37552058 | DOI:10.1099/jmm.0.001671 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 8, 2023 Category: Microbiology Authors: Dandan Yin Yan Guo Renru Han Yang Yang Demei Zhu Fupin Hu Source Type: research
Serum levels of type I interferon (IFN-I) is associated with the severity of COVID-19
Conclusion. These results demonstrate that macrophages and DCs are the main origination of IFN-I, and serum levels of IFN-I are positively associated with lymphopenia and cytokine storm, suggesting that IFN-α/β deteriorated the severity of COVID-19. Anti-interferon or IFN-I signalling block drugs are needed to treat ICU patients.PMID:37526406 | DOI:10.1099/jmm.0.001694 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2023 Category: Microbiology Authors: Yuting Sun Gang Wang Rongshuai Wang Liang Ren Zilin Yuan Yueping Liu Yuzhang Wu Rong Chen Yongwen Chen Bo Diao Source Type: research
Serum levels of type I interferon (IFN-I) is associated with the severity of COVID-19
Conclusion. These results demonstrate that macrophages and DCs are the main origination of IFN-I, and serum levels of IFN-I are positively associated with lymphopenia and cytokine storm, suggesting that IFN-α/β deteriorated the severity of COVID-19. Anti-interferon or IFN-I signalling block drugs are needed to treat ICU patients.PMID:37526406 | DOI:10.1099/jmm.0.001694 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2023 Category: Microbiology Authors: Yuting Sun Gang Wang Rongshuai Wang Liang Ren Zilin Yuan Yueping Liu Yuzhang Wu Rong Chen Yongwen Chen Bo Diao Source Type: research
Serum levels of type I interferon (IFN-I) is associated with the severity of COVID-19
Conclusion. These results demonstrate that macrophages and DCs are the main origination of IFN-I, and serum levels of IFN-I are positively associated with lymphopenia and cytokine storm, suggesting that IFN-α/β deteriorated the severity of COVID-19. Anti-interferon or IFN-I signalling block drugs are needed to treat ICU patients.PMID:37526406 | DOI:10.1099/jmm.0.001694 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2023 Category: Microbiology Authors: Yuting Sun Gang Wang Rongshuai Wang Liang Ren Zilin Yuan Yueping Liu Yuzhang Wu Rong Chen Yongwen Chen Bo Diao Source Type: research
Serum levels of type I interferon (IFN-I) is associated with the severity of COVID-19
Conclusion. These results demonstrate that macrophages and DCs are the main origination of IFN-I, and serum levels of IFN-I are positively associated with lymphopenia and cytokine storm, suggesting that IFN-α/β deteriorated the severity of COVID-19. Anti-interferon or IFN-I signalling block drugs are needed to treat ICU patients.PMID:37526406 | DOI:10.1099/jmm.0.001694 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2023 Category: Microbiology Authors: Yuting Sun Gang Wang Rongshuai Wang Liang Ren Zilin Yuan Yueping Liu Yuzhang Wu Rong Chen Yongwen Chen Bo Diao Source Type: research
Serum levels of type I interferon (IFN-I) is associated with the severity of COVID-19
Conclusion. These results demonstrate that macrophages and DCs are the main origination of IFN-I, and serum levels of IFN-I are positively associated with lymphopenia and cytokine storm, suggesting that IFN-α/β deteriorated the severity of COVID-19. Anti-interferon or IFN-I signalling block drugs are needed to treat ICU patients.PMID:37526406 | DOI:10.1099/jmm.0.001694 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2023 Category: Microbiology Authors: Yuting Sun Gang Wang Rongshuai Wang Liang Ren Zilin Yuan Yueping Liu Yuzhang Wu Rong Chen Yongwen Chen Bo Diao Source Type: research
