Comparative characterisation of the biofilm-production abilities of Staphylococcus epidermidis isolated from human skin and platelet concentrates.
CONCLUSION: Positive selection of S. epidermidis biofilm-producers during blood donation and PC manufacturing was not observed. Interestingly, ica-negative biofilm-producers seem to be negatively affected by skin disinfection, blood processing and PC storage. Furthermore, this study shows that S. epidermidis adopts a biofilm-forming phenotype in PCs regardless of its genetic background or origin. PMID: 29388540 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Taha M, Kohnen C, Mallya S, Kou Y, Zapata A, Ramirez-Arcos S Tags: J Med Microbiol Source Type: research

Chlamydia trachomatis inhibits the production of pro-inflammatory cytokines in human PBMCs through induction of IL-10.
CONCLUSION: IL-10 is up-regulated in an ERK- and p38-dependent fashion in stimulated human PBMCs. IL-10 inhibits the production of pro-inflammatory cytokines by activating the JAK/STAT signalling pathway. PMID: 29388547 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Du K, Zhou M, Li Q, Liu XZ Tags: J Med Microbiol Source Type: research

Comparative activity of tedizolid and glycopeptide combination therapies for the treatment of Staphylococcus aureus infections: an in vitro and in vivo evaluation against strains with reduced susceptibility to glycopeptides.
CONCLUSIONS: TED had good in vitro activity versus MRSA including those with reduced susceptibility to glycopeptides. Although bacteriostatic, it was effective in the G. mellonella model and superior to TEIC in the treatment of GISA. Although this supports the use of TED for MRSA and GISA, the TEIC/RIF combination also warrants further study. PMID: 29300156 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 4, 2018 Category: Microbiology Authors: Betts JW, Abdul Momin HF, Phee LM, Wareham DW Tags: J Med Microbiol Source Type: research

Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes.
CONCLUSION: Mutations or deletions in ramR, leading to the overexpression of RamA predominated in clinical MDR E. aerogenes isolates and were associated with a higher-level of expression of efflux pump components. It was hypothesised that mutations in ramR, and the self-regulating region proximal to ramA, probably altered the binding properties of the RamR repressor; thereby producing the MDR phenotype. Consequently, mutability of RamR may play a key role in predisposing E. aerogenes towards the emergence of a MDR phenotype. PMID: 29297851 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 3, 2018 Category: Microbiology Authors: Molitor A, James CE, Fanning S, Pagès JM, Davin-Regli A Tags: J Med Microbiol Source Type: research

Predominance of influenza A(H3N2) viruses during the 2016/2017 season in Bulgaria.
CONCLUSION: The results of this study confirm the genetic variability of circulating influenza viruses, particularly A(H3N2), and the need for continued antigenic and molecular surveillance. PMID: 29297852 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 3, 2018 Category: Microbiology Authors: Korsun N, Angelova S, Trifonova I, Tzotcheva I, Mileva S, Voleva S, Georgieva I, Perenovska P Tags: J Med Microbiol Source Type: research

Rapid discrimination of Staphylococcus epidermidis genotypes in a routine clinical microbiological laboratory using single nucleotide polymorphisms in housekeeping genes.
CONCLUSION: This method can rapidly establish whether S. epidermidis strains belong to different genotypes. It can be used in patients with S. epidermidis infections, as an aid in outbreak investigations and to select strains for investigation with more discriminatory methods, saving workload and costs. Results may be obtained the same day as culture results. Its strength lies mainly in indicating differences, as some STs may have the same melt profile. Changes in S. epidermidis epidemiology may warrant alterations in the inclusion of SNPs. We believe this method can reduce the threshold for performing genotyping analysis ...
Source: Journal of Medical Microbiology - January 2, 2018 Category: Microbiology Authors: Larssen KW, Nor A, Bergh K Tags: J Med Microbiol Source Type: research

Actinobacillus pleuropneumoniae culture supernatant antiviral effect against porcine reproductive and respiratory syndrome virus occurs prior to the viral genome replication and transcription through actin depolymerization.
CONCLUSION: App culture supernatant antiviral effect against PRRSV takes place early during PRRSV infection. Results suggest that App culture supernatant antiviral effect may take place via the activation of cofilin, which induces actin depolymerization and subsequently, probably affects PRRSV endocytosis. Other experiments are needed to fully validate this latest hypothesis. PMID: 29293082 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 2, 2018 Category: Microbiology Authors: Hernandez Reyes Y, Provost C, Traesel CK, Jacques M, Gagnon CA Tags: J Med Microbiol Source Type: research

Microbiological profile and clinical characteristics of diabetic foot infection in northern China: a retrospective multicentre survey in the Beijing area.
CONCLUSIONS: Enterobacteriaceae were the most frequently isolated organisms in our area. Older patients were more likely to suffer from Gram-negative rod infections. Gram-negative rods show high sensitivity to piperacillin/tazobactam, carbapenems and amikacin. PMID: 29293083 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 2, 2018 Category: Microbiology Authors: Li X, Qi X, Yuan G, Ju S, Yu Z, Deng W, Liu Y, Li Y, Bu X, Ding M, Li Q, Guo X Tags: J Med Microbiol Source Type: research

Trials and Tribulations of Enteric Parasitology in the Molecular Era.
PMID: 29265996 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 21, 2017 Category: Microbiology Authors: O' Leary JK, Blake L, Corcoran D, Sleator RD, Lucey B Tags: J Med Microbiol Source Type: research

β LACTA testing may not improve treatment decisions made with MALDI-TOF MS-informed antimicrobial stewardship advice for patients with Gram-negative bacteraemia: a prospective comparative study.
CONCLUSIONS: Under our test conditions, BLT, when used in conjunction with MALDI-TOF MS and AMST advice, did not allow a significant optimization of the antimicrobial prescription made on the basis AMST advice only. However, the impact of BLT should be evaluated in a population with high prevalence of ESBL-producing Enterobacteriaceae and/or when treatment choices are not made by infectious disease specialists. PMID: 29265997 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 21, 2017 Category: Microbiology Authors: Dépret F, Aubry A, Fournier A, Charles-Nelson A, Katsahian S, Compain F, Mainardi JL, Fernandez-Gerlinger MP Tags: J Med Microbiol Source Type: research

Prevalence, healthcare resource utilization and overall burden of fungal meningitis in the United States.
CONCLUSION: Our study characterizes the largest longitudinal cohort of fungal meningitis in the United States. Importantly, the health economic impact and long-term morbidity from these infections are quantified and reviewed. The healthcare resource utilization of fungal meningitis patients in the United States is substantial. PMID: 29244019 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 15, 2017 Category: Microbiology Authors: Charalambous LT, Premji A, Tybout C, Hunt A, Cutshaw D, Elsamadicy AA, Yang S, Xie J, Giamberardino C, Pagadala P, Perfect JR, Lad SP Tags: J Med Microbiol Source Type: research

Antibiotic susceptibility pattern of Neisseria gonorrhoeae strains isolated from five cities in India during 2013-2016.
CONCLUSION: Our data suggest that the disc diffusion method which is both cost effective and more feasible, can effectively be used routinely for monitoring antibiotic susceptibility in N. gonorrhoeae, in limited resource countries like India. We demonstrate the emergence of decreased susceptibility to ceftriaxone and cefixime and threshold levels of resistance to azithromycin in India. This underscores the importance of maintaining continued surveillance for antibiotic resistance in N. gonorrhoeae and a potential requirement for strategic change in guidelines in the not so distant future. PMID: 29231153 [PubMed - as ...
Source: Journal of Medical Microbiology - December 12, 2017 Category: Microbiology Authors: Kulkarni SV, Bala M, Muqeeth SA, Sasikala G, Nirmalkar AP, Thorat R, Kambli H, Sawant J, Risbud A, Gangakhedkar RR, Godbole SV Tags: J Med Microbiol Source Type: research

Association between sequevar and antibiotic treatment outcome in patients with Mycobacterium abscessus complex infections in Japan.
CONCLUSION: Patients infected with multiple sequevars and genotypes were more likely to exhibit treatment failure and/or recurrence. The precise identification of subspecies and analyses of mycobacterial characteristics may help to predict treatment outcomes in patients with M. abscessus complex lung disease. PMID: 29227218 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 11, 2017 Category: Microbiology Authors: Yoshida S, Tsuyuguchi K, Kobayashi T, Tomita M, Inoue Y, Hayashi S, Suzuki K Tags: J Med Microbiol Source Type: research

Accuracy and reproducibility of the Etest to detect drug-resistant Neisseria gonorrhoeae to contemporary treatment.
CONCLUSION: Overall, the Etest method yielded reproducible MIC values within each laboratory with the azithromycin and ceftriaxone MIC results consistent to the reference agar dilution method while the cefixime result tended to provide a lower MIC value. PMID: 29219803 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 8, 2017 Category: Microbiology Authors: Papp JR, Rowlinson MC, O'Connor NP, Wholehan J, Razeq JH, Glennen A, Ware D, Iwen PC, Lee LV, Hagan C Tags: J Med Microbiol Source Type: research

Comparative typing analyses of clinical and environmental strains of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast.
CONCLUSION: These results provide new information on the ecology and epidemiology of the C. neoformans/C. gattii species complex in Ivory Coast. PMID: 29214970 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 7, 2017 Category: Microbiology Authors: Kassi FK, Bellet V, Drakulovski P, Krasteva D, Roger F, Valérie BA, Aboubakar T, Doumbia A, Kouakou GA, Delaporte E, Reynes J, Yavo W, Menan HIE, Bertout S Tags: J Med Microbiol Source Type: research

Insights into the candidacidal mechanism of Ctn[15-34] - a carboxyl-terminal, crotalicidin-derived peptide related to cathelicidins.
CONCLUSION: Overall, Ctn[15-34] displays a synergistic antifungal activity with AMB, an effect that can be further developed into a multi-target therapeutic option with other antimycotics currently in use. PMID: 29214974 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 7, 2017 Category: Microbiology Authors: Cavalcante CSP, de Aguiar FLL, Fontenelle ROS, de Menezes RRPPB, Martins AMC, Falcão CB, Andreu D, Rádis-Baptista G Tags: J Med Microbiol Source Type: research

A hemin auxotrophic Enterobacter cloacae clinical isolate with increased resistance to carbapenems and aminoglycosides.
Abstract Small-colony variants (SCVs) were obtained from an Enterobacter cloacae clinical isolate in Okinawa, Japan. One variant showed auxotrophy for hemin with a deletion of 20 365 nucleotides, dosC-ydiK-mmuP-mmuM-tauA-tauB-tauC-tauD-hemB-yaiT-yaiV-ampH-yddQ-sbmA-yaiW-yaiY-yaiZ, including hemB, and was more resistant to aminoglycosides and carbapenems, but more susceptible to aztreonam, than the parent strain. PMID: 29205135 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 5, 2017 Category: Microbiology Authors: Tada T, Uechi K, Nakasone I, Miyazato Z, Shinzato T, Shimada K, Tsuchiya M, Kirikae T, Fujita J Tags: J Med Microbiol Source Type: research

Virulence genes in isolates of Klebsiella pneumoniae from the UK during 2016, including among carbapenemase gene-positive hypervirulent K1-ST23 and 'non-hypervirulent' types ST147, ST15 and ST383.
CONCLUSION: Our results highlight a combination of virulence and resistance genes, which could lead to untreatable invasive infections. PMID: 29205138 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 5, 2017 Category: Microbiology Authors: Turton JF, Payne Z, Coward A, Hopkins KL, Turton JA, Doumith M, Woodford N Tags: J Med Microbiol Source Type: research

Listeria monocytogenes cells under nutrient deprivation showed reduced ability to infect the human intestinal cell line HT-29.
CONCLUSION: Our results suggest that when L. monocytogenes are in refrigerated post-processing environments that are unable to support their growth they may increase their resistance to nutritional stress and may decrease their virulence. This should be considered when performing risk assessments for refrigerated ready-to-eat (RTE) foods. PMID: 29185940 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 29, 2017 Category: Microbiology Authors: Araujo V, Neves E, Silva AC, Martins APL, Brito LC Tags: J Med Microbiol Source Type: research

Cell-wall recycling and synthesis in Escherichia coli and Pseudomonas aeruginosa - their role in the development of resistance.
Abstract The bacterial cell-wall that forms a protective layer over the inner membrane is called the murein sacculus - a tightly cross-linked peptidoglycan mesh unique to bacteria. Cell-wall synthesis and recycling are critical cellular processes essential for cell growth, elongation and division. Both de novo synthesis and recycling involve an array of enzymes across all cellular compartments, namely the outer membrane, periplasm, inner membrane and cytoplasm. Due to the exclusivity of peptidoglycan in the bacterial cell-wall, these players are the target of choice for many antibacterial agents. Our current under...
Source: Journal of Medical Microbiology - November 29, 2017 Category: Microbiology Authors: Dhar S, Kumari H, Balasubramanian D, Mathee K Tags: J Med Microbiol Source Type: research

Isolation and molecular characterization of group B Streptococcus from laboratory Long-Evans rats (Rattus norvegicus) with and without invasive group B streptococcal disease.
CONCLUSIONS: To our knowledge, this is the first investigation of genotypic and phenotypic features of rat-derived GBS strains and their comparison to human- and other animal-derived GBS strains. Since GBS commonly colonizes commercially available rats, its exclusion as a potential pathogen for immunocompromised or stressed animals is recommended. PMID: 29160197 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 21, 2017 Category: Microbiology Authors: Bodi Winn C, Bakthavatchalu V, Esmail MY, Feng Y, Dzink-Fox J, Richey L, Perkins SE, Nordberg EK, Fox JG Tags: J Med Microbiol Source Type: research

Molecular epidemiology and antimicrobial susceptibility of Clostridium difficile isolated from hospitals during a 4-year period in China.
CONCLUSION: MLST typing revealed a wide variety of STs causing CDI, while ST-54 was the most common ST. All of the isolates were susceptible to metronidazole and vancomycin, while the resistance rates varied for the other antibiotics tested. There were no changes in the trends for the STs and antibiotic susceptibility profiles over 4 years. PMID: 29160203 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 21, 2017 Category: Microbiology Authors: Chen YB, Gu SL, Shen P, Lv T, Fang YH, Tang LL, Li LJ Tags: J Med Microbiol Source Type: research

In vitro and in vivo activity of fosfomycin alone and in combination with rifampin and tigecycline against Gram-positive cocci isolated from surgical wound infections.
In conclusion, the efficacy of fosfomycin combinations, also confirmed in our in vivo model, may suggest new directions in the treatment of infected skin and a possible alternative way to control bacterial skin infection. PMID: 29154746 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 20, 2017 Category: Microbiology Authors: Simonetti O, Morroni G, Ghiselli R, Orlando F, Brenciani A, Xhuvelaj L, Provinciali M, Offidani A, Guerrieri M, Giacometti A, Cirioni O Tags: J Med Microbiol Source Type: research

Disseminated methicillin-susceptible Staphylococcus aureus infection.
DISCUSSION: This case illustrates a severe clinical presentation and management of MSSA infections. PMID: 29154747 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 20, 2017 Category: Microbiology Authors: Perkins DR, Hall JA, Lopez LM, Cahuayme-Zuniga LJ Tags: J Med Microbiol Source Type: research

Characterization of biofilm formation by Enterococcus faecalis isolates derived from urinary tract infections in China.
CONCLUSION: ST16 classification was positively associated with strong biofilm formation in E. faecalis as was agg, while cylA was associated with weak biofilm formation. PMID: 29148361 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 17, 2017 Category: Microbiology Authors: Zheng JX, Bai B, Lin ZW, Pu ZY, Yao WM, Chen Z, Li DY, Deng XB, Deng QW, Yu ZJ Tags: J Med Microbiol Source Type: research

Characterization of clinically isolated thymidine-dependent small-colony variants of Escherichia coli producing extended-spectrum β-lactamase.
CONCLUSION: Mutation in the thyA gene causes morphological abnormalities in the colonies and cells of E. coli, as well as inducing thymidine auxotrophy. In addition, TD-SCVs horizontally transmit plasmids encoding resistance genes. It is important to detect TD-SCVs based on their characteristics because they serve as reservoirs of transferable antibiotic resistance plasmids. PMID: 29143727 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 16, 2017 Category: Microbiology Authors: Negishi T, Matsumoto T, Horiuchi K, Kasuga E, Natori T, Matsuoka M, Ogiwara N, Sugano M, Uehara T, Nagano N, Honda T Tags: J Med Microbiol Source Type: research

Outbreak of Pichia kudriavzevii fungaemia in a neonatal intensive care unit.
CONCLUSION: This study emphasizes the importance of accurate identification of the aetiological agent of sepsis and vigilant monitoring for the possibility of an outbreak in NICUs. PMID: 29134932 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 14, 2017 Category: Microbiology Authors: Nagarathnamma T, Chunchanur SK, Rudramurthy SM, Vineetha KR, Ramamurthy K, Joseph J, Ambica R Tags: J Med Microbiol Source Type: research

Susceptibility of vancomycin-resistant and -sensitive Enterococcus faecium obtained from Danish hospitals to benzalkonium chloride, chlorhexidine and hydrogen peroxide biocides.
CONCLUSION: VRE faecium from Danish hospitals demonstrated decreased susceptibility towards benzalkonium chloride and chlorhexidine compared to VSE faecium, where the use of chlorhexidine is particularly heavy in the hospital environment. These findings suggest that biocide tolerance may characterize VRE faecium isolated in Danish hospitals. PMID: 29134935 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 14, 2017 Category: Microbiology Authors: Alotaibi SMI, Ayibiekea A, Pedersen AF, Jakobsen L, Pinholt M, Gumpert H, Hammerum AM, Westh H, Ingmer H Tags: J Med Microbiol Source Type: research

Short-term incubation of positive blood cultures in brain-heart infusion broth accelerates identification of bacteria by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry.
CONCLUSION: Short-term enrichment of BCs in BHI accelerates identification of a number of bacterial species by MALDI-TOF MS. Further prospective studies are needed to validate our method and gauge its potential clinical impact on the management of bloodstream bacterial infections. PMID: 29134938 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 14, 2017 Category: Microbiology Authors: Torres I, Gimenez E, Pascual T, Bueno F, Huntley D, Martínez M, Navarro D Tags: J Med Microbiol Source Type: research

The nursing home elder microbiome stability and associations with age, frailty, nutrition and physical location.
CONCLUSION: These findings support the conclusion that a signature 'NH' microbiota may exist that is affected by the residents' age, frailty, nutritional status and physical location. PMID: 29134939 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 14, 2017 Category: Microbiology Authors: Haran JP, Bucci V, Dutta P, Ward D, McCormick B Tags: J Med Microbiol Source Type: research

Intramammary infusion of a live culture of Lactococcus lactis in ewes to treat staphylococcal mastitis.
CONCLUSION: Under our experimental conditions, the L. lactis treatment led to a transient clearance of the pathogen in the gland, but also caused mild to moderate clinical cases of mastitis. We believe it is still early to implement bacterial formulations as alternatives in treating mastitis in ruminants and further experimentation is needed. PMID: 29134942 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 14, 2017 Category: Microbiology Authors: Mignacca SA, Dore S, Spuria L, Zanghì P, Amato B, Duprè I, Armas F, Biasibetti E, Camperio C, Lollai SA, Capucchio MT, Cannas EA, Di Marco Lo Presti V, Marianelli C Tags: J Med Microbiol Source Type: research

Bovine leukaemia virus genotypes 5 and 6 are circulating in cattle from the state of S ão Paulo, Brazil.
CONCLUSION: BLV is still important in Brazil and this research should be continued. PMID: 29120299 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 9, 2017 Category: Microbiology Authors: Gregory L, Carrillo Gaeta N, Araújo J, Matsumiya Thomazelli L, Harakawa R, Ikuno AA, Hiromi Okuda L, de Stefano E, Pituco EM Tags: J Med Microbiol Source Type: research

Antimicrobial susceptibility, virulence potential and sequence types associated with Arcobacter strains recovered from human faeces.
CONCLUSION: The fact that epidemiological unrelated strains show the same ST indicates that other techniques with higher resolution should be developed to effectively recognize the infection source. Resistance to ciprofloxacin, one of the antibiotics recommended for the treatment of Arcobacter intestinal infections, demonstrated in 10.7 % of the strains, indicates the importance of selecting the most appropriate effective treatment. PMID: 29120301 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 9, 2017 Category: Microbiology Authors: Pérez-Cataluña A, Tapiol J, Benavent C, Sarvisé C, Gómez F, Martínez B, Terron-Puig M, Recio G, Vilanova A, Pujol I, Ballester F, Rezusta A, Figueras MJ Tags: J Med Microbiol Source Type: research

Patient age as a factor of antibiotic resistance in methicillin-resistant Staphylococcus aureus.
CONCLUSION: Antibiotics that target DNA synthesis result in a progressively higher number of resistant isolates among the older population. The results emphasize the importance of patient age on antibiotic selection as a preventive measure to reduce the rate of resistant infections in each susceptible population. This pattern suggests that physicians should take into consideration patient age as another factor in determining the best antibiotic regiment with the aim of curtailing the emergence of newer resistant phenotypes in the future. PMID: 29116037 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 8, 2017 Category: Microbiology Authors: Garcia A, Delorme T, Nasr P Tags: J Med Microbiol Source Type: research

Resistance mechanisms of linezolid-nonsusceptible enterococci in Korea: low rate of 23S rRNA mutations in Enterococcus faecium.
CONCLUSION: A low rate of 23S rRNA mutations and the absence of known linezolid-resistance mechanisms in the majority of E. faecium isolates suggest regional differences in the mechanisms of linezolid resistance and the possibility of additional mechanisms. PMID: 29111969 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 7, 2017 Category: Microbiology Authors: Lee SM, Huh HJ, Song DJ, Shim HJ, Park KS, Kang CI, Ki CS, Lee NY Tags: J Med Microbiol Source Type: research

Genome sequence analysis of emm89 Streptococcus pyogenes strains causing infections in Scotland, 2010-2016.
CONCLUSIONS: The clade 3 clone causes virtually all-contemporary emm89 infections in Scotland. These findings add Scotland to a growing list of countries of Europe and North America where, by whole genome sequencing, emm89 clade 3 strains have been demonstrated to be the cause of an ongoing epidemic of invasive infections and to be genetically related due to descent from a recent common progenitor. PMID: 29099690 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 3, 2017 Category: Microbiology Authors: Beres SB, Olsen RJ, Ojeda Saavedra M, Ure R, Reynolds A, Lindsay DSJ, Smith AJ, Musser JM Tags: J Med Microbiol Source Type: research

Phylogenetic distribution and expression of a penicillin-binding protein homologue, Ear and its significance in virulence of Staphylococcus aureus.
CONCLUSION: Our data suggests that despite being highly expressed in a USA300 genetic background, Ear is not a significant contributor to virulence in that strain. PMID: 29099691 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 3, 2017 Category: Microbiology Authors: Singh VK, Ring RP, Aswani V, Stemper ME, Kislow J, Ye Z, Shukla SK Tags: J Med Microbiol Source Type: research

Parvovirus B19 in HIV+ adult patients with different CD4+ lymphocyte counts.
Abstract PURPOSE: Human parvovirus B19 (B19V) can cause anemia in immunocompromised patients. We aimed to investigate the presence of B19V in HIV+ adults with different CD4+ T cell counts, to recognise the frequency of B19V in these different conditions and its possible association with anemia. METHODOLOGY: We studied B19V specific IgM, IgG and DNA in 98 HIV+ patients and in 52 healthy individuals. HIV load, CD4+ counts and haemoglobin level were also determined in the patients. RESULTS: No individual in the control group had detectable IgM, 41/52 (78.8 %) had IgG and 5/52 (9.6 %...
Source: Journal of Medical Microbiology - November 2, 2017 Category: Microbiology Authors: Pedranti MS, Rodriguez-Lombardi G, Bracciaforte R, Romano N, Lujan P, Ricchi B, Mautino J, Adamo MP Tags: J Med Microbiol Source Type: research

High mortality of bloodstream infection outbreak caused by carbapenem-resistant P. aeruginosa producing SPM-1 in a bone marrow transplant unit.
CONCLUSIONS: The predominant clone belonging to ST277 showed high mortality. Carbapenem use was the only risk factor associated with CRPa-BSI. This finding is a wake-up call for the need to improve management in BMT units. PMID: 29095142 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 2, 2017 Category: Microbiology Authors: Chaves L, Tomich LM, Salomão M, Leite GC, Ramos J, Martins RR, Rizek C, Neves P, Batista MV, Amigo U, Guimaraes T, Levin AS, Costa SF Tags: J Med Microbiol Source Type: research

Clostridium difficile infections in a university hospital in Greece are mainly associated with PCR ribotypes 017 and 126.
CONCLUSION: The CDI incidence was comparable to that in other European countries. The hypervirulent PCR ribotype 027 was not found, whereas ribotypes 017 and 126 predominated. Most CDI cases were in patients who used antibiotics, emphasizing that antimicrobial stewardship should be considered as a cornerstone for the prevention of CDI. PMID: 29087273 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 31, 2017 Category: Microbiology Authors: Kachrimanidou M, Tsachouridou O, Ziogas IA, Christaki E, Protonotariou E, Metallidis S, Skoura L, Kuijper E Tags: J Med Microbiol Source Type: research

Regarding flagellar expression in clinical isolates of non-typeable Haemophilus influenzae.
rd PR PMID: 29087280 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 31, 2017 Category: Microbiology Authors: Bossé JT, Langford PR Tags: J Med Microbiol Source Type: research

Chain-length-specific anti-Candida activity of cationic lipo-oxazoles: a new class of quaternary ammonium compounds.
CONCLUSION: A novel class of QACs, called cationic lipo-oxazoles, was tested and found to exhibit anti-fungal activity against planktonic cells as well as biofilms of Candida. PMID: 29076804 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 27, 2017 Category: Microbiology Authors: Jain T, Muktapuram PR, Pochampalli S, Sharma K, Pant G, Mitra K, Bathula SR, Banerjee D Tags: J Med Microbiol Source Type: research

Investigation of next-generation sequencing data of Klebsiella pneumoniae using web-based tools.
CONCLUSION: Applying appropriate web-based online tools to NGS data enables the rapid extraction of comprehensive information that can be used for more efficient diagnosis and treatment of patients, while data processing is free of charge, easy and time-efficient. PMID: 29068275 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 25, 2017 Category: Microbiology Authors: Brhelova E, Antonova M, Pardy F, Kocmanova I, Mayer J, Racil Z, Lengerova M Tags: J Med Microbiol Source Type: research

An investigation of antifungal stewardship programmes in England.
CONCLUSION: Although a minority of Trusts conduct dedicated AFS programmes, nearly half include AFS as part of routine AMS activities. Cost issues are the main driver for AFS, followed by clinical need. The availability of rapid diagnostics and clinical support could help increase AFS initiatives. PMID: 29068278 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 25, 2017 Category: Microbiology Authors: Micallef C, Ashiru-Oredope D, Hansraj S, Denning DW, Agrawal SG, Manuel RJ, Schelenz S, Guy R, Muller-Pebody B, Patel R, Howard P, Hopkins S, Johnson E, Enoch DA Tags: J Med Microbiol Source Type: research

Prevalence of respiratory viruses in Iranian patients with idiopathic pulmonary fibrosis.
CONCLUSION: Determining a correlation between the viruses and IPF is not an easy task, and therefore, this will require more research. In addition, the CLART Pneumovir DNA array can be considered as a useful method for simultaneous detection of several viral respiratory infections. PMID: 29068280 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 25, 2017 Category: Microbiology Authors: Keyvani H, Moghoofei M, Bokharaei-Salim F, Mostafaei S, Javad Mousavi SA, Monavari SH, Esghaei M Tags: J Med Microbiol Source Type: research

Detection of Bartonella in cat scratch disease using a single-step PCR assay kit.
CONCLUSION: The performance of this commercial kit suggests that it could be a suitable alternative to our in-house PCR assay, highlighting the importance of the molecular methods used to diagnose CSD. PMID: 29068281 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 25, 2017 Category: Microbiology Authors: Hobson C, Le Brun C, Beauruelle C, Maakaroun-Vermesse Z, Mereghetti L, Goudeau A, Lanotte P Tags: J Med Microbiol Source Type: research

Genomic diversity of human adenovirus type 3 isolated in Fukui, Japan over a 24-year period.
In this study, analysis of 75 isolates of HAdV- 3 collected over a 24-year period in Fukui revealed: (1) the emergence of three novel genome types (HAdV-3a52, HAdV-3a53 and HAdV-3a54) and two known genome types (HAdV-3a and HAdV-3a54); (2) the spectrum of diseases caused by individual genome types and their major involvement in the paediatric age population; and (3) the co-circulation and replacement of genome types as a usual phenomenon. The rising number of HAdV-3 genome types indicates that the genetic variation of HAdV-3 is more than other HAdVs. Considering the clinical importance of HAdV-3 infection, its genetic dive...
Source: Journal of Medical Microbiology - October 25, 2017 Category: Microbiology Authors: Adhikary AK Tags: J Med Microbiol Source Type: research

Dynamic HIV-1 genetic recombination and genotypic drug resistance among treatment-experienced adults in northern Ghana.
CONCLUSION: HIV-1 shows high genetic diversity and on-going viral genetic recombination in the study region. Nearly 42 % of the patients studied harboured a drug-resistant virus. The study underscores the need for continued surveillance of HIV-1 subtype diversity; and of drug-resistance patterns to guide selection of second-line regimens in northern Ghana. PMID: 29068286 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 25, 2017 Category: Microbiology Authors: Nii-Trebi NI, Brandful JAM, Ibe S, Sugiura W, Barnor JS, Bampoh PO, Yamaoka S, Matano T, Yoshimura K, Ishikawa K, Ampofo WK Tags: J Med Microbiol Source Type: research

Significant spread of extensively drug-resistant Acinetobacter baumannii genotypes of clonal complex 92 among intensive care unit patients in a university hospital in southern Iran.
CONCLUSION: This report demonstrates the occurrence and potential spread of closely related XDR genotypes of A. baumannii CC92 within a university hospital in southern Iran. These genotypes were found in the majority of the investigated isolates, showed high prevalence of blaOXA-23 and integron class 1, and were associated with stay in the intensive care unit. Very few treatment options remain for healthcare-adapted XDR A. baumannii, and hence effective measures are desperately needed to reduce the spread of these strains and resultant infections in the healthcare setting. PMID: 29058650 [PubMed - as supplied by publi...
Source: Journal of Medical Microbiology - October 23, 2017 Category: Microbiology Authors: Saffari F, Monsen T, Karmostaji A, Azimabad FB, Widerström M Tags: J Med Microbiol Source Type: research

Comparison of in-house IgM and IgG ELISAs for the serodiagnosis of melioidosis in Malaysia.
This study compared the efficacy of in-house IgM and IgG ELISA methods using a local B. pseudomallei strain. The diagnostic accuracy of the in-house IgG ELISA was better than that of the IgM ELISA: sensitivity (IgG: 84.71 %, IgM: 76.14 %) and specificity (IgG: 93.64 %, IgM: 90.17 %); positive predictive value (IgG: 86.75 %, IgM: 79.76 %) and negative predictive value (IgG: 92.57 %, IgM: 89.66 %); likelihood ratio (LR) [IgG: 13.32, IgM: 7.75 (LR+); IgG: 0.16, IgM: 0.26 (LR-)], and was supported by the observation of the absorbance value in comparisons between culture and serology sampling. In-house IgG ELISA...
Source: Journal of Medical Microbiology - October 19, 2017 Category: Microbiology Authors: Hii SYF, Ali NA, Ahmad N, Amran F Tags: J Med Microbiol Source Type: research