Whole-genome sequencing analysis of multidrug-resistant Mycobacterium tuberculosis from Java, Indonesia.
Conclusion. Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia. PMID: 32579102 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 23, 2020 Category: Microbiology Authors: Tania T, Sudarmono P, Kusumawati RL, Rukmana A, Pratama WA, Regmi SM, Kaewprasert O, Chaiprasert A, Chongsuvivatwong V, Faksri K Tags: J Med Microbiol Source Type: research

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases.
This study aims to explore the molecular epidemiology and clinical features of HBoV isolates in patients with respiratory diseases.Methodology. Retrospectively, between 2018 and 2020, 5941 respiratory samples from patients with respiratory diseases were screened for respiratory viruses using multiplex real-time PCR. Samples that were positive for HBoV were then subjected to NP1 and VP1/PV2 phylogenetic analysis.Results. HBoV was detected in 1.9 % of the patients, with a peak incidence of infection among children
Source: Journal of Medical Microbiology - June 23, 2020 Category: Microbiology Authors: Madi NM, Al-Adwani A Tags: J Med Microbiol Source Type: research

Shiga toxin-producing Escherichia coli haemolytic uraemic syndrome (STEC-HUS): diagnosis, surveillance and public-health management in England.
PMID: 32552986 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 17, 2020 Category: Microbiology Authors: Jenkins C, Byrne L, Vishram B, Sawyer C, Balasegaram S, Ahyow L, Johnson S Tags: J Med Microbiol Source Type: research

Evaluating the use of a 22-pathogen TaqMan array card for rapid diagnosis of respiratory pathogens in intensive care.
Abstract Introduction. Pneumonia is highly prevalent in intensive care units (ICUs), with high associated mortality. Empirical treatment prioritizes breadth of coverage while awaiting laboratory diagnosis, often at the expense of antimicrobial stewardship. Microarrays use multiple parallel polymerase chain reactions to enable a rapid syndromic approach to laboratory diagnosis.Aim. To evaluate the clinical and laboratory implications of introducing a bespoke 22-pathogen TaqMan Array Card (TAC) for rapid pathogen detection in deep respiratory samples from adult ICUs.Methodology. TAC results from all ICU patients pro...
Source: Journal of Medical Microbiology - June 17, 2020 Category: Microbiology Authors: Jones NK, Conway Morris A, Curran MD, Parmar S, Sule O, Enoch DA, Aliyu SH, Zhang H, Jalal H, Navapurkar V, Murphy ME Tags: J Med Microbiol Source Type: research

Antimicrobial resistance profiles of diarrhoeagenic Escherichia coli isolated from travellers returning to the UK, 2015-2017.
Conclusions. Increasing MDR, along with resistance to the fluroquinolones and the third-generation cephalosporins, in DEC causing travellers' diarrhoea provides further evidence for the need to restrict the use of antimicrobial agents and continuous monitoring. PMID: 32530393 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 11, 2020 Category: Microbiology Authors: Boxall MD, Day MR, Greig DR, Jenkins C Tags: J Med Microbiol Source Type: research

Characterization of Ba813 harbouring Bacillus cereus in patients with haematological malignancy and hospital environments at a medical centre in Japan.
Conclusion. Ba813(+) B. cereus, which are genetically closely related to B. anthracis, were abundant in a haematological ward. Ba813(+) B. cereus with high motility and biofilm formation abilities may spread easily in hospital environments, and could become a hospital-acquired infection. PMID: 32530394 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 11, 2020 Category: Microbiology Authors: Aoyagi T, Kishihara Y, Ogawa M, Ito Y, Tanaka S, Kobayashi R, Tokuda K, Kaku M Tags: J Med Microbiol Source Type: research

Pandemic planning: plotting a course through the coronawars.
Abstract The biological motor behind the current coronavirus pandemic has placed microbiology on a global stage, and given its practitioners a role among the architects of recovery. Planning for a return to normality or the new normal is a complex, multi-agency task for which healthcare scientists may not be prepared. This paper introduces a widely used military planning framework known as the Joint Military Appreciation Process, and outlines how it can be applied to deal with the next phase of the COVID-19 pandemic. Recognition of SARS-CoV-2's critical attributes, targetable vulnerabilities, and its most likely a...
Source: Journal of Medical Microbiology - June 10, 2020 Category: Microbiology Authors: Inglis TJJ Tags: J Med Microbiol Source Type: research

Correlation between cervical HPV DNA detection and HPV16 seroreactivity measured with L1-only and L1+L2 viral capsid antigens.
Conclusion. HPV16 DNA positivity and HPV16 seroreactivity are strongly correlated. L1+L2 VLPs perform better than L1-only VLPs for detecting IgG antibodies to HPV16 in women infected with HPV16 or other Alpha-9 HPV species. This study advances our understanding of humoral immune responses against HPV16 by providing insights about the influence of VLP antigen composition to measure humoral immune response against naturally acquired HPV infection. PMID: 32510304 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 7, 2020 Category: Microbiology Authors: Trevisan A, Candeias JMG, Thomann P, Villa LL, Franco EL, Trottier H, Study Group FTL Tags: J Med Microbiol Source Type: research

Pseudomonas 2019 meeting report.
PMID: 32490791 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 2, 2020 Category: Microbiology Authors: Hardie KR, Mathee K, Schweizer HP, Dietrich LE, Welch M, de Kievit T, Nguyen D, Kivisaar M, Dandekar AA, McDougald D, Winstanley C Tags: J Med Microbiol Source Type: research

Improving the recovery and detection of bloodstream pathogens from blood culture.
Conclusion. The efficiency of bacterial recovery can vary significantly between different methods and thereby can have a critical impact on downstream analysis. The low-speed serum-separation method offered a simple and effective means of recovering common BSI pathogens from blood culture and will be further investigated for use in the rapid detection of bacteraemia and susceptibility testing in clinical practice. PMID: 32490793 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 2, 2020 Category: Microbiology Authors: Falconer K, Hammond R, Gillespie SH Tags: J Med Microbiol Source Type: research

Does concomitant bacteraemia hide the fungi in blood cultures? An in vitro study.
Conclusion. Blood culture bottles including selective fungal media that can allow selective growth of fungi and earlier detection of some species should be preferred in addition to non-selective blood culture bottles, especially in specific patient populations. Further, the use of selective agar plates such as inhibitory mould agar may contribute to the solution of this problem in clinical laboratories. PMID: 32490795 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 2, 2020 Category: Microbiology Authors: Oz Y, Onder S, Alpaslan E, Durmaz G Tags: J Med Microbiol Source Type: research

Efficient differentiation of Nocardia farcinica, Nocardia cyriacigeorgica and Nocardia beijingensis by high-resolution melting analysis using a novel locus.
In conclusion, the developed molecular assay allows simultaneous detection and differentiation of N. farcinica, N. cyriacigeorgica and N. beijingensis with high sensitivity and specificity. PMID: 32478652 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 31, 2020 Category: Microbiology Authors: Xu S, Hou X, Li D, Sun L, Li M, Ji X, Wang X, Li Z Tags: J Med Microbiol Source Type: research

Emerging tick-borne pathogens of public health importance: a mini-review.
Abstract Ticks are the most important vectors of human pathogens, leading to increased public health burdens worldwide. Tick-borne pathogens include viruses (e.g. tick-borne encephalitis and Powassan); bacteria, such as the causative agents of Lyme disease, spotted fever rickettsiosis and human anaplasmosis; and malaria-like protozoan parasites causing babesiosis. Tick-borne diseases are emerging due to the geographical expansion of their tick vectors, especially in the northern hemisphere. Two examples of this phenomenon are Ixodes scapularis and Amblyomma americanum, which have expanded their ranges in the USA i...
Source: Journal of Medical Microbiology - May 31, 2020 Category: Microbiology Authors: Rochlin I, Toledo A Tags: J Med Microbiol Source Type: research

Optimizing a real-time PCR assay for rapid detection of Candida auris in nasal and axillary/groin samples.
Conclusion . The optimized procedure was used to test 1414 patient surveillance samples. The real-time PCR detected all culture-positive samples with 100 % sensitivity and 100 % specificity. PMID: 32478655 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 31, 2020 Category: Microbiology Authors: Malczynski M, Dowllow N, Rezaeian S, Rios J, Dirnberger L, Zembower JA, Zhu A, Qi C Tags: J Med Microbiol Source Type: research

Potential RNA-dependent RNA polymerase inhibitors as prospective therapeutics against SARS-CoV-2.
Conclusion. Quinupristin represents a potential anti-SARS-CoV-2 therapeutic. At present, we have no evidence that this drug is effective against SARS-CoV-2 but expect that the biomedical community will expeditiously follow up on our in silico findings. PMID: 32469301 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 28, 2020 Category: Microbiology Authors: Pokhrel R, Chapagain P, Siltberg-Liberles J Tags: J Med Microbiol Source Type: research

HIV serology false positivity among expatriates from Africa: a screening dilemma.
In conclusion, HIV-RNA and pro-viral DNA testing is recommended for HIV screening of individuals from high-prevalence regions coming to low-prevalence regions. PMID: 32469303 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 28, 2020 Category: Microbiology Authors: Al-Kindi H, Al-Jardani A Tags: J Med Microbiol Source Type: research

Pseudomonas aeruginosa biofilm formation on endotracheal tubes requires multiple two-component systems.
Conclusions. Our study established an in vitro method for studying P. aeruginosa biofilm formation on the ETT surfaces. We also identified novel ETT-specific TCSs that could serve as targets to prevent biofilm formation on indwelling devices frequently used in clinical settings. PMID: 32459613 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 26, 2020 Category: Microbiology Authors: Badal D, Jayarani AV, Kollaran MA, Kumar A, Singh V Tags: J Med Microbiol Source Type: research

Simple differentiation of Salmonella Typhi, Paratyphi and Choleraesuis from Salmonella species using the eazyplex TyphiTyper LAMP assay.
Conclusion. The eazyplex TyphiTyper is very easy to perform and allows the rapid identification of TS and S. Choleraesuis isolates. PMID: 32459614 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 26, 2020 Category: Microbiology Authors: Rödel J, Edel B, Braun SD, Ehricht R, Simon S, Fruth A, Löffler B Tags: J Med Microbiol Source Type: research

Emergence of azole resistant-Aspergillus fumigatus infections during STAT3-deficiency.
Conclusions. The emergence of azole-resistant A. fumigatus infections in STAT3-deficiency dramatically impacts both curative and prophylactic antifungal strategies. Physicians following patients with primary immune-deficiencies should be aware of this emerging problem as it complicates management of the patient. PMID: 32459615 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 26, 2020 Category: Microbiology Authors: Danion F, Duréault A, Gautier C, Senechal A, Persat F, Bougnoux ME, Givel C, Couderc LJ, Lortholary O, Garcia-Hermoso D, Lanternier F Tags: J Med Microbiol Source Type: research

Darunavir inhibits Cryptococcus neoformans/Cryptococcus gattii species complex growth and increases the susceptibility of biofilms to antifungal drugs.
a MFG Abstract Introduction. Cryptococcus species are pathogens commonly associated with cases of meningoencephalitis in individuals who are immunosuppressed due to AIDS.Aim. The aim was to evaluate the effects of the antiretroviral darunavir alone or associated with fluconazole, 5-flucytosine and amphotericin B against planktonic cells and biofilms of Cryptococcus species.Methodology. Susceptibility testing of darunavir and the common antifungals against 12 members of the Cryptococcus neoformans/Cryptococcus gattii species complex was evaluated by broth microdilution. The interaction between darunavir and antifun...
Source: Journal of Medical Microbiology - May 26, 2020 Category: Microbiology Authors: Brilhante RSN, Silva JAT, Araújo GDS, Pereira VS, Gotay WJP, Oliveira JS, Guedes GMM, Pereira-Neto WA, Castelo-Branco DSCM, Cordeiro RA, Sidrim JJC, Rocha MFG Tags: J Med Microbiol Source Type: research

Genetic diversity of influenza A viruses circulating in Bulgaria during the 2018-2019 winter season.
Conclusion. The results of this study showed the genetic diversity of circulating influenza viruses and the need for continuous antigenic and molecular surveillance. PMID: 32459617 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 26, 2020 Category: Microbiology Authors: Korsun N, Daniels R, Angelova S, Ermetal B, Grigorova I, Voleva S, Trifonova I, Kurchatova A, McCauley J Tags: J Med Microbiol Source Type: research

Evaluation of the usefulness of selected methods for the detection of carbapenemases in Klebsiella strains.
Conclusion. There was complete agreement between the carbapenemases detected by the genetic method and the results obtained with phenotypic methods. PMID: 32459618 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 26, 2020 Category: Microbiology Authors: Sękowska A, Bogiel T, Kaczmarek A Tags: J Med Microbiol Source Type: research

In vitro bactericidal activity of a carbohydrate polymer with zinc oxide for the treatment of chronic wounds.
Conclusions. Together with its previously shown anti-inflammatory properties, the bactericidal activity of CPZO gives it the potential to be a first-line therapeutic option for chronic various ulcers and, possibly, other chronic ulcers, preventing or controlling microbial infections, and leading to the healing of such complicated chronic ulcers. PMID: 32459619 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 26, 2020 Category: Microbiology Authors: Bello-López JM, López-Ornelas A, Vilchis-Rangel RE, Ribas-Aparicio RM, Del-Moral P, Donis-Rocandio JE, Cueto J, Aparicio-Ozores G, Moreno J Tags: J Med Microbiol Source Type: research

Staphylococcus aureus nasal isolates may have the same genetic profile in atopic dermatitis paediatric patients and their close contacts.
This study aimed to detect S. aureus from nares of AD patients and their family contacts, as well as to evaluate the antimicrobial resistance, virulence and clonality of these isolates. Among the 48 family groups investigated, 30 groups were selected, as both the child and his/her respective contact had methicillin-sensitive S. aureus (MSSA) (24 cases; 54 MSSA isolates) or methicillin-resistant S. aureus (MRSA) isolates (6 cases; 13 MRSA isolates). All MRSA isolates carried SCCmec IV. S. aureus carrying PVL genes were detected in 60 % of patients. Pulsed-field gel electrophoresis (PFGE) analysis was performed for 31 isol...
Source: Journal of Medical Microbiology - May 19, 2020 Category: Microbiology Authors: Cavalcante FS, Alvarenga CVDCG, Saintive S, Dios Abad E, Carvalho Ferreira D, Netto Dos Santos KR Tags: J Med Microbiol Source Type: research

In vitro inhibitory effect of statins on planktonic cells and biofilms of the Sporothrix schenckii species complex.
Conclusion. These results highlight the antifungal and antibiofilm potential of statins against S. schenckii complex species. PMID: 32427094 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 18, 2020 Category: Microbiology Authors: Brilhante RSN, Fonseca XMQC, Pereira VS, Araújo GDS, Oliveira JS, Garcia LGS, Rodrigues AM, Camargo ZP, Pereira-Neto WA, Castelo-Branco DSCM, Cordeiro RA, Sidrim JJC, Rocha MFG Tags: J Med Microbiol Source Type: research

Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification.
Conclusion. C. albicans was the main cause of yeast-derived fungaemia in this study. Future prospective studies are warranted to closely monitor the epidemiological landscape of yeast species causing BSIs in Iran. The superiority of 21-plex PCR over automated Vitek 2 indicates its potential clinical utility as an alternative identification tool use in developing countries. PMID: 32368996 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 4, 2020 Category: Microbiology Authors: Kord M, Salehi M, Khodavaisy S, Hashemi SJ, Daie Ghazvini R, Rezaei S, Maleki A, Elmimoghaddam A, Alijani N, Abdollahi A, Doomanlou M, Ahmadikia K, Rashidi N, Pan W, Boekhout T, Arastehfar A Tags: J Med Microbiol Source Type: research

IncN3 and IncHI2 plasmids with an In1763 integron carrying bla IMP-1 in carbapenem-resistant Enterobacterales clinical isolates from the UK.
Conclusion. Highly similar IncN3 plasmids were found in a range of Enterobacterales, mostly K. pneumoniae and the Enterobacter cloacae complex, from three of four London hospitals, with the same In1763 integron carrying bla IMP-1 also being found in IncHI2 plasmids and chromosomally. These plasmids carried multiple elements facilitating self-transmission. Strain typing alone was not sufficient to investigate cross-infection among this set of isolates, many of which appeared to be unrelated until plasmid analysis was undertaken, and vice versa. PMID: 32368998 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 4, 2020 Category: Microbiology Authors: Turton JF, Davies F, Taori SK, Turton JA, Smith SL, Sajedi N, Wootton M Tags: J Med Microbiol Source Type: research

Ability of quantitative PCR to discriminate Pneumocystis jirovecii pneumonia from colonization.
Abstract Introduction. Pneumocystis jirovecii pneumonia (PCP) is a severe disease affecting immunocompromised patients. Diagnosis is difficult due to the low sensitivity of direct examination and inability to grow the pathogen in culture. Quantitative PCR in bronchoalveolar lavage fluid (BAL) has high sensitivity, but limited specificity for distinguishing PCP from colonization.Aim. To assess the performance of an in-house quantitative PCR to discriminate between PCP and colonization.Methodology. This was a single-centre retrospective study including all patients with a positive PCR result for P. jirovecii in BAL ...
Source: Journal of Medical Microbiology - May 4, 2020 Category: Microbiology Authors: Perret T, Kritikos A, Hauser PM, Guiver M, Coste AT, Jaton K, Lamoth F Tags: J Med Microbiol Source Type: research

Diversity of navel microbiome in young adults.
Conclusion. Our findings indicate that the navel skin microbiota of young adults has a core microbiota of Corynebacterium and Staphylococcus. We also noted the presence of a significant number of opportunistic pathogens. A minor gender difference in the abundance of individual organisms was also observed. PMID: 32369006 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 4, 2020 Category: Microbiology Authors: Shah S, Donze-Reiner T, Shah V Tags: J Med Microbiol Source Type: research

Sodium salicylate interferes with quorum-sensing-regulated virulence in chronic wound isolates of Pseudomonas aeruginosa in simulated wound fluid.
Conclusions. Pseudomonas aeruginosa from chronic wound infections showed different virulence properties. While very few strains showed no QS activity, approximately half were highly virulent and produced QS signals, suggesting that the targeting of QS is a viable and relevant strategy for infection control. NaSa showed activity as a QS-inhibitor by lowering the virulence phenotypes and QS signals at both transcriptional and extracellular levels. PMID: 32320374 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 21, 2020 Category: Microbiology Authors: Gerner E, Almqvist S, Werthén M, Trobos M Tags: J Med Microbiol Source Type: research

Logic in the time of coronavirus.
Abstract Much has happened here since the local news media trumpeted the first Australian COVID-19 fatality, and stirred up a medieval fear of contagion. We now need to take a step back to examine the logic underlying the use of our limited COVID-19 countermeasures. Emerging infectious diseases by their nature, pose new challenges to the diagnostic-treatment-control nexus, and push our concepts of causality beyond the limits of the conventional Koch-Henle approach to aetiology. We need to use contemporary methods of assessing causality to ensure that clinical, laboratory and public health measures draw on a ration...
Source: Journal of Medical Microbiology - April 21, 2020 Category: Microbiology Authors: Inglis TJJ Tags: J Med Microbiol Source Type: research

Molecular identification of Nocardia species causing endophthalmitis using multilocus sequence analysis (MLSA): a 10-year perspective.
Conclusions. N. farcinica is the major pathogen, and this is the first report to identify N. otitidiscaviarum, N. amikacinitolerans and N. higoensis as causing endophthalmitis. Overall, visual outcomes were mostly poor even after aggressive management. PMID: 32310744 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 20, 2020 Category: Microbiology Authors: Gnanam H, Rajapandian SGK, Gunasekaran R, Roshni Prithiviraj S, Ravindran RS, Sen S, Prajna L Tags: J Med Microbiol Source Type: research

Point of care testing of Influenza A/B and RSV in an adult respiratory assessment unit is associated with improvement in isolation practices and reduction in hospital length of stay.
Abstract Introduction. Every winter seasonal influenza and other viral respiratory infections increase pressure on the health services and are associated with nosocomial infection and morbidity.Aim. To compare provision of point-of-care (POC) testing with laboratory-based testing for influenza and RSV detection on an adult respiratory assessment unit to assess the impact on isolation practices and length of stay (LOS).Methodology. Prospective interrupted 'on-off' study in adults admitted to the respiratory unit between December 2018 and April 2019 with a suspected respiratory tract infection. Nasopharyngeal sample...
Source: Journal of Medical Microbiology - April 5, 2020 Category: Microbiology Authors: Berry L, Lansbury L, Gale L, Carroll AM, Lim WS Tags: J Med Microbiol Source Type: research

LPS stimulation during HCV infection induces MMP/TIMP1 imbalance in macrophages.
Conclusion. Increased LPS stimulation of macrophage during HCV antigen-induced endotoxin cross-tolerance contributes to MMP/TIMP1 imbalance and collagen I deposition. PMID: 32242792 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 2, 2020 Category: Microbiology Authors: Fan C, Zhang X, Zhang P, Zhao J, Shen H, Zhang Y, Wu X, Jia Z, Wang Y Tags: J Med Microbiol Source Type: research

Extracellular DNA controls expression of Pseudomonas aeruginosa genes involved in nutrient utilization, metal homeostasis, acid pH tolerance and virulence.
Abstract Introduction. Pseudomonas aeruginosa grows in extracellular DNA (eDNA)-enriched biofilms and infection sites. eDNA is generally considered to be a structural biofilm polymer required for aggregation and biofilm maturation. In addition, eDNA can sequester divalent metal cations, acidify growth media and serve as a nutrient source.Aim. We wanted to determine the genome-wide influence on the transcriptome of planktonic P. aeruginosa PAO1 grown in the presence of eDNA.Methodology. RNA-seq analysis was performed to determine the genome-wide effects on gene expression of PAO1 grown with eDNA. Transcriptional lu...
Source: Journal of Medical Microbiology - April 2, 2020 Category: Microbiology Authors: Lewenza S, Johnson L, Charron-Mazenod L, Hong M, Mulcahy-O'Grady H Tags: J Med Microbiol Source Type: research

Bacterial quantification in tissue homogenates from in vivo pharmacodynamic studies using growth curves.
Abstract Introduction. Quantification of bacterial load in tissue homogenates in in vivo pharmacodynamic studies is cumbersome and time-consuming.Aim. We therefore developed a new method for quantifying bacterial load in tissue homogenates of animals treated with a β-lactam and β-lactamase inhibitor using growth curves.Methods. The log10 colony-forming units (c.f.u.) ml-1 of 184 thigh and lung homogenates from female CD-1 mice infected intranasally and intramuscularly with 4 Pseudomonas aeruginosa, 4 Klebsiella pneumoniae, 3 Enterobacter cloacae and 2 Escherichia coli strains treated with a β-lactam...
Source: Journal of Medical Microbiology - March 30, 2020 Category: Microbiology Authors: Georgiou PC, Mouton JW, Pournaras S, Meletiadis J Tags: J Med Microbiol Source Type: research

In vitro antivirulence activity of baicalin against Clostridioides difficile.
Conclusion. Our results suggest that baicalin could potentially be used to control C. difficile, and warrant future studies in vivo. PMID: 32216868 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 26, 2020 Category: Microbiology Authors: Pellissery AJ, Vinayamohan PG, Venkitanarayanan K Tags: J Med Microbiol Source Type: research

Epidemiology of Enterobacter cloacae strains producing a carbapenemase or metallo-beta-lactamase in Vietnamese clinical settings in 2014-2017.
Conclusions. Several prevalent clones of carbapenem-resistant E. cloacae have circulated within Vietnamese hospitals. Adequate measures are needed to prevent their further spread. PMID: 32216869 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 26, 2020 Category: Microbiology Authors: Miyoshi-Akiyama T, Ohmagari N, Phuong TT, Huy NQ, Anh NQ, Van Thanh D, Thuy PTP, Kirikae T, Nhung PH, Takemoto N Tags: J Med Microbiol Source Type: research

The bacterial neurometabolic signature of the gut microbiota of young children with autism spectrum disorders.
Conclusion. For the first time, the neurometabolic signature of the gut microbiota of young children with ASD is presented. The data can help to provide a comparative assessment of the transcriptional and metabolomic activity of the identified genes. PMID: 32213246 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 25, 2020 Category: Microbiology Authors: Averina OV, Kovtun AS, Polyakova SI, Savilova AM, Rebrikov DV, Danilenko VN Tags: J Med Microbiol Source Type: research

Cronobacter sakazakii induces necrotizing enterocolitis by regulating NLRP3 inflammasome expression via TLR4.
Conclusion. We found that C. sakazakii upregulates NF-κB via TLR4/MyD88 to promote activation of the NLRP3 inflammasome, leading to the up-regulation of downstream caspase-1, release of IL-1β, GSDMD-mediated pyroptosis and development of NEC. These findings clarify the mechanisms by which C. sakazakii contributes to NEC. PMID: 32209170 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 24, 2020 Category: Microbiology Authors: Chen Z, Zhang Y, Lin R, Meng X, Zhao W, Shen W, Fan H Tags: J Med Microbiol Source Type: research

A pooled analysis of patients with wound infections in the Phase 3 REVIVE trials: randomized, double-blind studies to evaluate the safety and efficacy of iclaprim versus vancomycin for treatment of acute bacterial skin and skin structure infections.
Conclusion. Based on early clinical response, iclaprim achieved non-inferiority to vancomycin with a similar safety profile in patients with wound infections suspected or confirmed as caused by Gram-positive pathogens. Iclaprim may be a valuable treatment option for wound infections. PMID: 32195649 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 19, 2020 Category: Microbiology Authors: Noviello S, Corey GR, Holland TL, Lodise T, O'Riordan W, Wilcox MH, File TM, Dryden M, Balser B, Scaramucci A, Torres A, Huang DB Tags: J Med Microbiol Source Type: research

Hydroxyethoxy phenyl butanone, a new cosmetic preservative, does not cause bacterial cross-resistance to antimicrobials.
Conclusion. Data produced on the propensity of a molecule to induce bacterial resistance is useful and appropriate when launching a new preservative. PMID: 32186482 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 17, 2020 Category: Microbiology Authors: Wesgate R, Menard-Szczebara F, Khodr A, Cupferman S, Maillard JY Tags: J Med Microbiol Source Type: research

Antimicrobial resistance prevalence in commensal Escherichia coli from broilers, fattening turkeys, fattening pigs and veal calves in European countries and association with antimicrobial usage at country level.
Abstract The aim of this article is to report on antimicrobial resistance (AMR) in commensal Escherichia coli from livestock from several European countries. The relationships with antimicrobial usage (AMU) at country level and harmonized indicators to cover the most relevant AMR aspects for human health in animal production were also investigated. E. coli were isolated in faeces from broilers and fattening pigs (from nine countries), and fattening turkeys and veal calves (from three countries) and screened against a fixed antimicrobial panel. AMU data were collected at farm and average treatment incidences strati...
Source: Journal of Medical Microbiology - March 17, 2020 Category: Microbiology Authors: Ceccarelli D, Hesp A, van der Goot J, Joosten P, Sarrazin S, Wagenaar JA, Dewulf J, Mevius DJ, Effort Consortium OBOT Tags: J Med Microbiol Source Type: research

Repurposing bioactive compounds for treating multidrug-resistant pathogens.
Conclusion. Although these compounds' potential for repurposing is limited by their toxicity, our results contribute to this growing field and provide a simple road map for using C. elegans for preliminary testing of known bioactive compounds with predicted antimicrobial activity. PMID: 32163353 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 11, 2020 Category: Microbiology Authors: Hummell NA, Kirienko NV Tags: J Med Microbiol Source Type: research

Phenotypic and molecular characterization of macrolide resistance mechanisms among Streptococcus pneumoniae isolated in Tunisia.
Conclusion. This study shows that macrolide resistance among S. pneumoniae isolated in Tunisia is mainly related to target site modification. Our observations demonstrate a high degree of genetic diversity and capsular types among strains resistant to macrolides. PMID: 32159507 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 10, 2020 Category: Microbiology Authors: Midouni Ayadi B, Mehiri E, Draoui H, Ghariani A, Essalah L, Raoult D, Fournier PE, Slim-Saidi LN Tags: J Med Microbiol Source Type: research

Rapid molecular testing for Staphylococcus aureus bacteraemia improves clinical management.
Conclusion. Rapid and accurate detection of S. aureus with the Xpert MRSA/SA BC assay in positive blood culture bottles allowed earlier targeted patient management. Negative Xpert results are suggestive of coagulase negative staphylococci, allowing de-escalation of antimicrobial therapy if clinically appropriate. PMID: 32141812 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 5, 2020 Category: Microbiology Authors: McHugh MP, Parcell BJ, MacKenzie FM, Templeton KE, Scottish Microbiology And Virology Network Smvn Molecular Diagnostics Evaluation Group Tags: J Med Microbiol Source Type: research

Low lineage diversity and increased virulence of group C Streptococcus dysgalactiae subsp. equisimilis.
Conclusion. These results suggest strongly that this predominance did not occur randomly but rather was due to adaptive mechanisms that culminated in increased colonization and other bacterial properties that might confer increased bacteria-host adaptability to cause disease. PMID: 32125264 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 2, 2020 Category: Microbiology Authors: Santos VL, Silva LG, Martini CL, Anjos IHV, Maia MM, Genteluci GL, Sant'Anna V, Ferreira AMA, Couceiro JNSS, Figueiredo AMS, Ferreira-Carvalho BT Tags: J Med Microbiol Source Type: research

Mutations in the two component regulator systems PmrAB and PhoPQ give rise to increased colistin resistance in Citrobacter and Enterobacter spp.
Conclusions. Stable colistin resistance may be acquired with no loss of fitness in these species. However, only select strains were able to adapt suggesting that acquisition of colistin resistance is dependent upon individual strain characteristics. PMID: 32125265 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 2, 2020 Category: Microbiology Authors: Wand ME, Sutton JM Tags: J Med Microbiol Source Type: research

Klebsiella variicola causing nosocomial transmission among neonates - an emerging pathogen?
Conclusion. K. variicola can cause hospital outbreaks of colonization and infection similar to other Klebsiella spp.Preliminary results from this study were presented at the 27th European Congress of Clinical Microbiology and Infectious Diseases, April 22-25, 2018, Vienna, Austria. PMID: 32125266 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 2, 2020 Category: Microbiology Authors: Piepenbrock E, Higgins PG, Wille J, Xanthopoulou K, Zweigner J, Jahn P, Reuter S, Skov R, Eichhorn J, Seifert H Tags: J Med Microbiol Source Type: research

Purification and characterization of nisin P produced by a strain of Streptococcus gallolyticus.
Conclusion. To the best of our knowledge, this study represents the first production, purification and characterization of nisin P. Further study of nisin P may reveal its potential for treating or preventing infections caused by antibiotic-resistant Gram-positive bacteria, or those evading vaccination regimens. PMID: 32125268 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 2, 2020 Category: Microbiology Authors: Aldarhami A, Felek A, Sharma V, Upton M Tags: J Med Microbiol Source Type: research