Transconjugation of erm(X) conferring high-level resistance of clindamycin for Cutibacterium acnes.
Abstract Cutibacterium acnes (C. acnes) can become an exacerbating factor in acne vulgaris. Clindamycin has been most frequently used for the treatment of inflammatory acne vulgaris. We studied clindamycin susceptibility and resistance determinants of C. acnes isolated from acne patients in Japan. The isolation rate of clindamycin-resistant C. acnes had significantly increased from 20.3 % in 2009-2010 to 44.1 % in 2016-2017. Strains carrying erm(X), which confers high-level resistance to clindamycin, had significantly increased from 1.4 to 11.8 %. Sequence analysis of the resistance determinant showed that e...
Source: Journal of Medical Microbiology - November 15, 2018 Category: Microbiology Authors: Aoki S, Nakase K, Hayashi N, Noguchi N Tags: J Med Microbiol Source Type: research

Corrigendum: Performance evaluation of five commercial assays in assessing seroprevalence of HEV antibodies among blood donors.
PMID: 30431419 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 15, 2018 Category: Microbiology Authors: Al-Absi ES, Al-Sadeq DW, Younis MH, Yassine HM, Abdalla OM, Mesleh AG, Hadwan TA, Amimo JO, Thalib L, Nasrallah GK Tags: J Med Microbiol Source Type: research

Fosfomycin: mechanisms and the increasing prevalence of resistance.
Abstract There are challenges regarding increased global rates of microbial resistance and the emergence of new mechanisms that result in microorganisms becoming resistant to antimicrobial drugs. Fosfomycin is a broad-spectrum bactericidal antibiotic effective against Gram-negative and certain Gram-positive bacteria, such as Staphylococci, that interfere with cell wall synthesis. During the last 40 years, fosfomycin has been evaluated in a wide range of applications and fields. Although numerous studies have been done in this area, there remains limited information regarding the prevalence of resistance. Therefore...
Source: Journal of Medical Microbiology - November 15, 2018 Category: Microbiology Authors: Aghamali M, Sedighi M, Zahedi Bialvaei A, Mohammadzadeh N, Abbasian S, Ghafouri Z, Kouhsari E Tags: J Med Microbiol Source Type: research

Comparison of susceptibility testing methods for determining the activity of colistin against Gram-negative bacilli of clinical origin.
CONCLUSION: We found BMAD to be very reliable for colistin MIC determination. Methods AD and DD should not be used for colistin susceptibility testing in P. aeruginosa isolates as these are associated with false-resistant and -susceptible results, respectively. PMID: 30422106 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 13, 2018 Category: Microbiology Authors: Haeili M, Kafshdouz M, Pishnian Z, Feizabadi MM, Martinez-Martinez L Tags: J Med Microbiol Source Type: research

In vivo activity of co-trimoxazole combined with colistin against Acinetobacter baumannii producing OXA-23 in a Galleria mellonella model.
CONCLUSIONS: Finally, the combination of colistin and cotrimoxazole appears to be a promising therapeutic option for the management of CRAB-associated infections. It is essential to assess the clinical application and the dose-response relationships of combinations such as colistin plus cotrimoxazole. PMID: 30422109 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 13, 2018 Category: Microbiology Authors: Khalil MAF, Moawad SS, Hefzy EM Tags: J Med Microbiol Source Type: research

Emergence of staphylococcal scalded skin syndrome associated with a new toxinogenic, methicillin-susceptible Staphylococcus aureus clone.
Abstract A sharp increase in staphylococcal scalded skin syndrome (SSSS) cases has been recorded in our settings since 2015, with 31 cases having been documented during the period 2014-2017. The molecular investigation of strains from the above period showed the emergence of a methicillin-susceptible, mupirocin- and fusidic acid-resistant Staphyloccocus aureus clone that belongs to the ST121 complex and carries both epidermolysin (eta/etb) genes. We concluded that the SSSS caused by the newly emerged, highly virulent community-associated-methicillin sensitive S. aureus strains that have been encountered lately is ...
Source: Journal of Medical Microbiology - November 12, 2018 Category: Microbiology Authors: Doudoulakakis A, Spiliopoulou I, Syridou G, Giormezis N, Militsopoulou M, Lebessi E, Tsolia M Tags: J Med Microbiol Source Type: research

Evaluation of an in-house MALDI-TOF MS rapid diagnostic method for direct identification of micro-organisms from blood cultures.
CONCLUSION: Our study provides an effective sample preparation method for the direct identification of pathogens from positive blood culture vials via Clin-TOF MS at a very low cost of about $0.5 per sample and with a short turnaround time of about 20 min. This will help clinicians make precise diagnoses and provide targeted prescriptions, reducing the risk of the potential development of resistance. PMID: 30418107 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 12, 2018 Category: Microbiology Authors: Huang YL, Sun QL, Li JP, Hu YY, Zhou HW, Zhang R Tags: J Med Microbiol Source Type: research

Molecular characterization of Staphylococcus argenteus in Myanmar: identification of novel genotypes/clusters in staphylocoagulase, protein A, alpha-haemolysin and other virulence factors.
CONCLUSION: The primary virulence factors (staphylocoagulase, protein A andalpha-haemolysin) as well as the SE-like toxins of S. argenteus were genetically discriminated from those of S. aureus, revealing the presence of the novel coa-type/subtype (coa-IXd, XIV) in S. argenteus. PMID: 30418108 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 12, 2018 Category: Microbiology Authors: Aung MS, San T, San N, Oo WM, Ko PM, Thet KT, Urushibara N, Kawaguchiya M, Sumi A, Kobayashi N Tags: J Med Microbiol Source Type: research

Evaluation of the Sofia Streptococcus pneumoniae FIA test for the detection of S. pneumoniae antigen in urine.
In conclusion, this study indicates that the Streptococcus pneumoniae FIA test shows similar sensitivity and specificity rates compared to the BinaxNOW SPN Antigen Card. PMID: 30403370 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 7, 2018 Category: Microbiology Authors: Euser SM, Badoux P, Kracht-Kosten L, Yzerman EPF Tags: J Med Microbiol Source Type: research

Evaluation of fast-acting bactericidal activity and substantivity of an antiseptic agent, olanexidine gluconate, using an ex vivo skin model.
CONCLUSION: OLG has fast-acting activity and substantivity, which are required properties for an antiseptic, and is useful for preventing infections. PMID: 30403371 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 7, 2018 Category: Microbiology Authors: Nishioka H, Nagahama A, Inoue Y, Hagi A Tags: J Med Microbiol Source Type: research

Low sensitivity of the MPT64 identification test to detect lineage 5 of the Mycobacterium tuberculosis complex.
Abstract PURPOSE: Differentiation of the Mycobacterium tuberculosis complex (MTBc) from non-tuberculous mycobacteria (NTM) is important for tuberculosis diagnosis and is a prerequisite for reliable phenotypic drug-resistance testing. We evaluated the performance of the rapid MPT64 antigen identification test for the detection of Mycobacterium africanum lineage 5 (MAF L5). METHODOLOGY: Smear-positive tuberculosis patients' sputa were included prospectively. Culture was performed on Löwenstein-Jensen medium and, when positive, the MPT64 test and the classical para-nitro benzoic acid susceptibility and heat...
Source: Journal of Medical Microbiology - November 2, 2018 Category: Microbiology Authors: Sanoussi CN, de Jong BC, Odoun M, Arekpa K, Ali Ligali M, Bodi O, Harris S, Ofori-Anyinam B, Yeboah-Manu D, Otchere ID, Asante-Poku A, Anagonou S, Gagneux S, Coscolla M, Rigouts L, Affolabi D Tags: J Med Microbiol Source Type: research

Bone and joint mycobacterial infection: a retrospective review of cases presenting to a UK district hospital.
CONCLUSIONS: BJTB requires a high index of clinical suspicion. BJTB should be considered in any patient with unexplained pain, fever and weight loss. The diagnosis is proven by aspiration and biopsy and should be undertaken as soon as possible for culture purposes, as microscopy alone can be negative. PMID: 30376445 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 30, 2018 Category: Microbiology Authors: Everden A, Mamo JP, Somasunderam D, McKee A, Brij SO, Enoch DA Tags: J Med Microbiol Source Type: research

The classical Bordetella species and MALDI-TOF technology: a brief experience.
CONCLUSION: MALDI-TOF might be a reliable tool for the identification of this group of bacteria when a combination of cutoff scores is used. This procedure allows us to increase the identification rates for the classical Bordetella species significantly; however, more studies will be required to determine the applicability of the method to other difficult-to-distinguish organisms. PMID: 30372410 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 29, 2018 Category: Microbiology Authors: Zintgraff J, Irazu L, Lara CS, Rodriguez M, Santos M Tags: J Med Microbiol Source Type: research

Everybody hands-on to avoid ESKAPE: effect of sustained hand hygiene compliance on healthcare-associated infections and multidrug resistance in a paediatric hospital.
CONCLUSIONS: A multimodal hand hygiene programme in a paediatric hospital in a middle-income country was effective in improving adherence and reducing HCAIs, CLABSIs and MDR-ESKAPE bloodstream infections. Sustaining hand hygiene adherence at a level of>60 % for one year limited MRSA clonal transmission. PMID: 30372411 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 29, 2018 Category: Microbiology Authors: De la Rosa-Zamboni D, Ochoa SA, Laris-González A, Cruz-Córdova A, Escalona-Venegas G, Pérez-Avendaño G, Torres-García M, Suaréz-Mora R, Castellanos-Cruz C, Sánchrez-Flores YV, Vázquez-Flores A, Águila-Torres R, Parra-Ortega I, Klünder-Klünder M Tags: J Med Microbiol Source Type: research

A 3-year retrospective analysis on the prevalence of antileptospiral antibodies among children in Chennai City, India.
CONCLUSION: This seroprevalence study suggested that leptospirosis occurs among children throughout the year in Chennai, India. This makes it almost mandatory to include it in the differential diagnosis for febrile illnesses in children. Early detection and treatment, together with control measures, may significantly reduce the spread of this endemic pathogen in this city. PMID: 30372412 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 29, 2018 Category: Microbiology Authors: Jacob SM, Geethalakshmi S, Karthikeyan S, Durairaj A, Gopal P, Ramamoorthy V, Arumugam G, Elumalai S Tags: J Med Microbiol Source Type: research

Rapid detection of mcr-1 by recombinase polymerase amplification.
CONCLUSION: RPA detection based on the mcr-1 gene was successfully applied in our study. The plasmid-mediated mcr-1 gene conferring colistin drug resistance has a strong ability to spread, suggesting the need to further strengthen the detection of this resistance gene in surveillance. Therefore, we require more sensitive detection methods than have previously been available and the RPA assay established in this study meets these detection needs. PMID: 30355422 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 24, 2018 Category: Microbiology Authors: Xu J, Wang X, Yang L, Kan B, Lu X Tags: J Med Microbiol Source Type: research

Human adipose tissue-derived mesenchymal stem/stromal cells adhere to and inhibit the growth of Staphylococcus aureus and Pseudomonas aeruginosa.
Abstract We have cultured and phenotyped human adipose tissue-derived mesenchymal stem/stromal cells (AT MSCs) and inoculated these cultures with bacteria common to infected skin wounds, i.e. Staphylococcus aureus and Pseudomonas aeruginosa. Cell interactions were examined by scanning electron microscopy (SEM), whilst bacterial growth was measured by colony forming unit (c.f.u.) and biofilm assays. AT MSCs appeared to attach to the bacteria and to engulf S. aureus. Significantly fewer bacterial c.f.u. were present in AT MSC : bacterial co-cultures compared with bacteria cultured alone. Antibacterial activity, ...
Source: Journal of Medical Microbiology - October 23, 2018 Category: Microbiology Authors: Wood CR, Al Dhahri D, Al Delfi I, Pickles NA, Sammons RL, Worthington T, Wright KT, Johnson WEB Tags: J Med Microbiol Source Type: research

A mutation in anti-sigma factor MAB_3542c may be responsible for tigecycline resistance in Mycobacterium abscessus.
In this study, we characterized 7C, a spontaneous mutant selected from tigecycline-susceptible Mycobacterium abscessus ATCC 19977. Whole-genome sequencing (WGS) was used to identify possible resistance determinants in this mutant. Compared to the wild-type, 7C demonstrated resistance to tigecycline as well as cross-resistance to imipenem, and had a slightly retarded growth rate. WGS and subsequent biological verifications showed that these phenotypes were caused by a point mutation in MAB_3542c, which encodes an RshA-like protein. In Mycobacterium tuberculosis, RshA is an anti-sigma factor that negatively regulates the hea...
Source: Journal of Medical Microbiology - October 23, 2018 Category: Microbiology Authors: Ng HF, Tan JL, Zin T, Yap SF, Ngeow YF Tags: J Med Microbiol Source Type: research

Characterization of a colistin-resistant Salmonella enterica 4,[5],12:i:- harbouring mcr-3.2 on a variant IncHI-2 plasmid identified in Canada.
This report provides further evidence of the emergence of a ST34 colistin-resistant clone. PMID: 30351266 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 23, 2018 Category: Microbiology Authors: Mulvey MR, Bharat A, Boyd DA, Irwin RJ, Wylie J Tags: J Med Microbiol Source Type: research

Staphylococcus aureus osteoarticular infections in children: an 8-year review of molecular microbiology, antibiotic resistance and clinical characteristics.
Abstract PURPOSE: To investigate the clinical, phenotypic and genotypic characteristics of Staphylococcus aureus strains causing osteoarticular infections in a large paediatric series. METHODOLOGY: Medical records of children who were hospitalized with the diagnosis of community-associated S. aureus (CA-SA) osteomyelitis and/or septic arthritis in the two major tertiary paediatric hospitals of Athens during an 8-year period (2007-2015) were reviewed, and S. aureus isolates were analysed regarding antimicrobial resistance, detection of pathogenicity genes and genotyping using SCCmec, agr typing, PFGE and MLST....
Source: Journal of Medical Microbiology - October 23, 2018 Category: Microbiology Authors: Bouras D, Doudoulakakis A, Tsolia M, Vaki I, Giormezis N, Petropoulou N, Lebessi E, Gennimata V, Tsakris A, Spiliopoulou I, Michos A Tags: J Med Microbiol Source Type: research

Editorial Conference Report 5th International One Health Congress, Saskatoon, Canada, 22-25 June 2018.
PMID: 30328807 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 17, 2018 Category: Microbiology Authors: Inglis TJJ Tags: J Med Microbiol Source Type: research

Binding of Pneumocystis carinii to the lung epithelial cell receptor HSPA5 (GRP78).
Abstract The importance of lung macrophages in Pneumocystis-host interaction is well known, but little is known about the initial binding/colonization of the airway epithelium. Our prior studies have documented cell-signalling events that occur following binding of the organisms to lung epithelial cells; however, the receptors that mediate Pneumocystis attachment to lung surfaces have not yet been fully defined. Using affinity chromatography, we identified heat shock protein 5 (HSPA5), also known as GRP78, as a potential host receptor that may have relevance in Pneumocystis lung colonization. Pneumocystis carinii ...
Source: Journal of Medical Microbiology - October 17, 2018 Category: Microbiology Authors: Kottom TJ, Hebrink DM, Limper AH Tags: J Med Microbiol Source Type: research

User and environment friendly direct agglutination test for the sero-diagnosis of visceral leishmaniasis: exclusion of formaldehyde and β-mercaptoethanol in test execution.
User and environment friendly direct agglutination test for the sero-diagnosis of visceral leishmaniasis: exclusion of formaldehyde and β-mercaptoethanol in test execution. J Med Microbiol. 2018 Oct 16;: Authors: Mahamoud A, Awad Y, Osman HA, El Agib A, Madi RR, Semiao-Santos SJ, El Harith A Abstract PURPOSE: Based on world-wide evaluation, the direct agglutination test (DAT) is now generally acknowledged as one of the leading diagnostics for visceral leishmaniasis (VL). To enhance more routine and mass application, but simultaneously ensure safety to both user and environment, further improvemen...
Source: Journal of Medical Microbiology - October 16, 2018 Category: Microbiology Authors: Mahamoud A, Awad Y, Osman HA, El Agib A, Madi RR, Semiao-Santos SJ, El Harith A Tags: J Med Microbiol Source Type: research

First use of whole-genome sequencing to investigate a cluster of Yersinia enterocolitica, Liverpool, United Kingdom, 2017.
Abstract PURPOSE: In May 2017 we were notified of a cluster of Yersinia enterocolitica-positive isolates from Liverpool. The purpose of this work was to investigate this cluster of cases and find a possible common source. We combined epidemiological information with whole-genome sequencing (WGS) results, which indicated that these cases were unlikely to be from the same source. This investigation provides evidence that WGS could be used to investigate future clusters of Y. enterocolitica cases. METHODS: A case was defined as a person with a laboratory-confirmed isolate of Y. enterocolitica, sampled in 2017, w...
Source: Journal of Medical Microbiology - October 16, 2018 Category: Microbiology Authors: Inns T, Flanagan S, Greig DR, Jenkins C, Seddon K, Chin T, Cartwright J Tags: J Med Microbiol Source Type: research

Verification of the Roche cobas ® 6800 PCR 200 µl and 500 µl protocols for the quantification of HIV-1 RNA, HBV DNA and HCV RNA and evaluation with COBAS® Ampliprep/COBAS® TaqMan® assays.
CONCLUSION: Cobas 6800 equipment and assays demonstrated excellent performance and correlated well with CAP/CTM assay results. PMID: 30325300 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 16, 2018 Category: Microbiology Authors: Tan NK, Carrington D, Pope CF Tags: J Med Microbiol Source Type: research

Efficacy of β-phenylethylamine as a novel anti-microbial and application as a liquid catheter flush.
Efficacy of β-phenylethylamine as a novel anti-microbial and application as a liquid catheter flush. J Med Microbiol. 2018 Oct 16;: Authors: Schroeder M, Horne SM, Prüß BM Abstract With this study, we introduce a liquid flush for catheters and other tubing-based applications that consists of a solution of β-phenylethylamine (PEA) in tryptic soy broth. The initial experiments in multiwell polystyrene plates were conducted with Escherichia coli K-12 to assess the effectiveness of PEA at reducing planktonic growth, as well as the biomass and adenosine triphosphate (ATP) content of bi...
Source: Journal of Medical Microbiology - October 16, 2018 Category: Microbiology Authors: Schroeder M, Horne SM, Prüß BM Tags: J Med Microbiol Source Type: research

Evaluation of the EntericBio CPE assay for the detection of carbapenemase-producing organisms.
Abstract Carbapenemase-producing organisms (CPOs) represent an increasing threat in healthcare facilities and detection of these organisms in the diagnostic laboratory can be challenging. The EntericBio CPE (EBCPE) real-time PCR assay (Serosep Ltd) was evaluated for the detection of NDM, KPC, OXA-48-like, VIM, IMP and GES carbapenemase genes from a panel of 145 multidrug-resistant organisms (29 NDM, 35 OXA-48, 21 VIM, 4 OXA-23, 3 KPC, 5 NDM+OXA-48, 3 GES-5, 1 OXA-23+NDM, 1 IMI, 2 IMP-1 and 41 multidrug-resistant carbapenemase-negative isolates). The EBCPE assay performed well, with 100 % sensitivity and specific...
Source: Journal of Medical Microbiology - October 15, 2018 Category: Microbiology Authors: Vanstone GL, Wey E, Mack D, Smith ER, Balakrishnan I Tags: J Med Microbiol Source Type: research

Mechanisms of carbapenem resistance in Acinetobacter pittii and Acinetobacter nosocomialis isolates from Thailand.
CONCLUSION: The major mechanisms of carbapenem resistance in A. pittii and A. nosocomialis were the production of OXA-23 and OXA-58. Overexpression of adeE played a role in carbapenem resistance in A. pittii. Since blaOXA-58 was found in carbapenem-susceptible A. pittii, using carbapenems in the treatment of A. pittii infection should be considered. PMID: 30311872 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 12, 2018 Category: Microbiology Authors: Singkham-In U, Chatsuwan T Tags: J Med Microbiol Source Type: research

Rapid identification of pathogens from positive blood culture bottles with the MinION nanopore sequencer.
CONCLUSION: We have established a rapid, portable and economical technique for the identification of pathogens in positive blood culture bottles through a novel MinION nanopore sequencer amplicon sequencing scheme, which replaces traditional Sanger sequencing. PMID: 30311873 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 12, 2018 Category: Microbiology Authors: Ashikawa S, Tarumoto N, Imai K, Sakai J, Kodana M, Kawamura T, Ikebuchi K, Murakami T, Mitsutake K, Maesaki S, Maeda T Tags: J Med Microbiol Source Type: research

Sub-lethal doses of surotomycin and vancomycin have similar effects on Clostridium difficile virulence factor production in vitro.
CONCLUSION: In summary, these findings demonstrate that surotomycin and vancomycin have similar effects on exotoxin production and sporulation by C. difficile in vitro. Furthermore, since spores contribute to recurrent infection, the ability of surotomycin to suppress spore formation may explain its ability to disrupt the reinfection cycle in the clinical setting. PMID: 30307842 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 11, 2018 Category: Microbiology Authors: Aldape MJ, Rice SN, Field KP, Bryant AE, Stevens DL Tags: J Med Microbiol Source Type: research

Are bacterial culture quantifications reliable? Comparative performance of the WASP automated inoculation instrument in the era of ISO 15189 accreditation.
CONCLUSION: We report the verification of the performance of the WASP instrument and describe a rapid procedure for achieving semi-quantitative cultures from BPSs and catheter tips. Quantitative interpretation of the bacterial cultures should be performed with caution. PMID: 30307844 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 11, 2018 Category: Microbiology Authors: Fihman V, Bleunven SC, Le Glaunec JM, Maillebuau F, De Rochebouet I, Nebbad-Lechani B, Desroches M, Decousser JW Tags: J Med Microbiol Source Type: research

Polymorphic variability in the exon 19 of the RB1 gene and its flanking intronic sequences in HPV16-associated precancerous lesions in the Greek population.
CONCLUSIONS: The A153104G nucleotide polymorphism did not emerge as a potential biomarker for the development of precancerous lesions in the Greek patients, while the accumulation of sequence variations in RB1 gene might influence patients' susceptibility towards the progression of cervical neoplasia. PMID: 30303478 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 10, 2018 Category: Microbiology Authors: Tsakogiannis D, Moschonas GD, Daskou M, Stylianidou Z, Kyriakopoulou Z, Kottaridi C, Dimitriou TG, Gartzonika C, Markoulatos P Tags: J Med Microbiol Source Type: research

Development of a rapid colorimetric multiplex PCR-reverse line blot for the detection and typing of 14 Chlamydia trachomatis genovars.
CONCLUSION: The mPCR-RLB assay is a rapid and sensitive methodology for the identification of C. trachomatis genovars associated with urogenital infections, trachoma or lymphogranuloma venereum diseases that can be implemented in clinical settings, helping to identify reinfections and treatment failures and establish the appropriate treatment course. PMID: 30303481 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 10, 2018 Category: Microbiology Authors: Molano M, Tabrizi SN, Phillips S, Danielewski J, Cornall A, Morre SA, Garland SM Tags: J Med Microbiol Source Type: research

Human papilloma virus genotype distribution and risk factor analysis amongst reproductive-age women in urban Gambia.
CONCLUSION: These data may have a significant public health impact as the HPV quadrivalent vaccine may be of limited value if the circulating non-HPV 16/18 HR-genotypes are responsible for cytological abnormalities of the cervix. PMID: 30299238 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 9, 2018 Category: Microbiology Authors: Bah Camara H, Anyanwu M, Wright E, Kimmitt PT Tags: J Med Microbiol Source Type: research

A carbapenem-resistant clinical isolate of Aeromonas hydrophila in Japan harbouring an acquired gene encoding GES-24 β-lactamase.
This study describes an isolate of Aeromonas hydrophila harbouring an acquired gene encoding the carbapenemase GES-24. This isolate was obtained from an inpatient in Okinawa, Japan, with no apparent record of travelling overseas. The minimum inhibitory concentrations of carbapenems against this isolate were 8 µg ml-1 for imipenem and 16 µg ml-1 for meropenem. Recombinant GES-24 hydrolyzed all of the tested β-lactams, including imipenem and meropenem. The genomic environment surrounding blaGES-24 was intI1-blaGES-24-aac(6')-IIc-qacEdelta1-sulI-orfX-tetR-tetE. This is the first re...
Source: Journal of Medical Microbiology - October 5, 2018 Category: Microbiology Authors: Uechi K, Tada T, Sawachi Y, Hishinuma T, Takaesu R, Nakama M, Nakasone I, Kirikae T, Fujita J Tags: J Med Microbiol Source Type: research

Corrigendum: Outer-membrane protein LptD (PA0595) plays a role in the regulation of alginate synthesis in Pseudomonas aeruginosa.
PMID: 30273113 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 1, 2018 Category: Microbiology Authors: Pandey S, Delgado C, Kumari H, Florez L, Mathee K Tags: J Med Microbiol Source Type: research

Microbiological and clinical characteristics of Group B Streptococcus isolates causing materno-neonatal infections: high prevalence of CC17/PI-1 and PI-2b sublineage in neonatal infections.
CONCLUSION: Our study highlights the high genotypic diversity of GBS strains causing materno-neonatal infections, and the CC17/PI-1 and PI-2b sublineages should be noted in neonatal infections. PMID: 30265233 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 28, 2018 Category: Microbiology Authors: Lu B, Wu J, Chen X, Gao C, Yang J, Li Y, Wang J, Zeng J, Fang Y, Wang D, Cui Y, Wang L Tags: J Med Microbiol Source Type: research

Clioquinol is a promising preventive morphological switching compound in the treatment of Candida infections linked to the use of intrauterine devices.
CONCLUSION: Our results indicate a potential application in terms of biomaterials for 8-hydroxyquinoline derivatives. Clioquinol could be used as a coating to prevent morphological switching and thus prevent biofilm formation. Furthermore, clioquinol may have future applications in the treatment of Candida infections linked to the use of IUDs. PMID: 30256190 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 26, 2018 Category: Microbiology Authors: Pippi B, Machado GDRM, Bergamo VZ, Alves RJ, Andrade SF, Fuentefria AM Tags: J Med Microbiol Source Type: research

An outbreak of Shigella boydii serotype 20 in January 2015 amongst United Kingdom healthcare workers involved in the Ebola response in Sierra Leone.
Abstract In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the ipaH gene in 10/12 faecal specimens, and Shigella boydii serotype 20 was isolated from 7 patients. A case control study was undertaken and analysed using multivariable logistic regression. Consumption of a coronation chicken lunch at the transit camp in Sierra Leone (SL) 24-48 h prior t...
Source: Journal of Medical Microbiology - September 25, 2018 Category: Microbiology Authors: Todkill D, Pudney R, Terrell A, Tuck J, Hutley E, Dallman TJ, Jenkins C, Manuel R, Wade M, Puleston R, Hawker J Tags: J Med Microbiol Source Type: research

Prevalence and antimicrobial susceptibilities of Acinetobacter baumannii and non-baumannii Acinetobacters from Terengganu, Malaysia and their carriage of carbapenemase genes.
Abstract A total of 153 non-repeat Acinetobacter spp. clinical isolates obtained in 2015 from Hospital Sultanah Nur Zahirah (HSNZ) in Terengganu, Malaysia, were characterized. Identification of the isolates at species level was performed by ribosomal DNA restriction analysis (ARDRA) followed by sequencing of the rpoB gene. The majority of the isolates (n=128; 83.7 %) were A. baumannii while the rest were identified as A. nosocomialis (n=16), A. calcoaceticus (n=5), A. soli (n=2), A. berezeniae (n=1) and A. variabilis (n=1). Multidrug resistance (MDR) was most prevalent in A. baumannnii (66.4 %) whereas only on...
Source: Journal of Medical Microbiology - September 25, 2018 Category: Microbiology Authors: Mohd Rani F, A Rahman NI, Ismail S, Abdullah FH, Othman N, Alattraqchi AG, Cleary DW, Clarke SC, Yeo CC Tags: J Med Microbiol Source Type: research

Molecular analysis of Streptococcus pyogenes strains isolated from patients with recurrent pharyngitis after oral amoxicillin treatment.
CONCLUSION: When recurrent pharyngitis emerges more than 40 days after the last treatment, penicillin can be prescribed again because reinfection is suspected. However, when recurrent pharyngitis takes place within 40 days after completing the treatment, alternative drugs should be considered for retreatment because the pharyngitis is likely to be due to reactivation. PMID: 30247119 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 24, 2018 Category: Microbiology Authors: Saito M, Hirose M, Ichinose H, Villanueva SYAM, Yoshida SI Tags: J Med Microbiol Source Type: research

Candida africana in recurrent vulvovaginal candidiasis (RVVC) patients: frequency and phenotypic and genotypic characteristics.
CONCLUSION: In our sample, C. africana was only isolated from 7.8 % of the patients with RVVC. While size polymorphisms in HPW1 genes allowed us to differentiate C. africana from C. albicans, no evidence of sequence variation within the Iranian C. africana isolates was observed. PMID: 30248002 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 24, 2018 Category: Microbiology Authors: Naeimi B, Mirhendi H, Khamisipour G, Sadeghzadeh F, Ahmadi B Tags: J Med Microbiol Source Type: research

Gluconeogenic growth of Vibrio cholerae is important for competing with host gut microbiota.
CONCLUSION: Gluconeogenesis biosynthesis is important for the successful colonization of V. cholerae in a niche that is full of competing microbiota. PMID: 30248003 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 24, 2018 Category: Microbiology Authors: Wang J, Xing X, Yang X, Jung IJ, Hao G, Chen Y, Liu M, Wang H, Zhu J Tags: J Med Microbiol Source Type: research

Determination and genome-wide analysis of Epstein-Barr virus (EBV) sequences in EBV-associated gastric carcinoma from Guangdong, an endemic area of nasopharyngeal carcinoma.
In conclusion, this study determined the EBV genomes in EBVaGC from Guangdong and performed a detailed genome-wide analysis of GC-EBV, which would be helpful for further understanding of the relationship between EBV genomic variation and EBVaGC carcinogenesis. PMID: 30239329 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 21, 2018 Category: Microbiology Authors: Chen JN, Zhou L, Qiu XM, Yang RH, Liang J, Pan YH, Li HF, Peng GR, Shao CK Tags: J Med Microbiol Source Type: research

Review of vaccination in pregnancy to prevent pertussis in early infancy.
Abstract Maternal pertussis vaccination has been introduced in several countries to protect infants from birth until routine infant vaccination takes place. This review assesses existing evidence on the effectiveness and safety of immunization in pregnancy. The search was finalized in April 2017 and was based on searches using several databases. The selection criteria included any experimental or observational study reporting on the immunogenicity, effectiveness or safety of vaccination with a pertussis-containing vaccine in pregnant women and their infants. Following de-duplication and exclusions, we identified 8...
Source: Journal of Medical Microbiology - September 17, 2018 Category: Microbiology Authors: Campbell H, Gupta S, Dolan GP, Kapadia SJ, Kumar Singh A, Andrews N, Amirthalingam G Tags: J Med Microbiol Source Type: research

Comparison of the Cepheid Xpert Xpress Flu/RSV Assay to in-house Flu/RSV triplex real-time RT-PCR for rapid molecular detection of Influenza A, Influenza B and Respiratory Syncytial Virus in respiratory specimens.
This study compared the performance of the commercially available Xpert Xpress Flu/RSV assay to an in-house FluAB/RSV triplex real-time RT-PCR assay for the detection of influenza A/B viruses and respiratory syncitial virus (RSV) from both nasopharyngeal aspirate (NPA) and nasopharyngeal flocked swab (NPS). A total of 20 external quality assurance (EQA) samples and 172 clinical respiratory samples were tested prospectively using both the Xpert Xpress Flu/RSV assay and the in-house FluAB/RSV triplex assay. For the EQA samples, concordance rate was 100 % when tested with both assays. For clinical samples, there was 100 %...
Source: Journal of Medical Microbiology - September 12, 2018 Category: Microbiology Authors: Ho YII, Wong AH, Lai RWM Tags: J Med Microbiol Source Type: research

Evaluating the microbial pattern of periprosthetic joint infections of the hip and knee.
CONCLUSION: Patients suffering PJI present a certain risk profile with many comorbidities, e.g. high age and obesity. The observed microbiological pattern demonstrates the rise of high-resistance pathogens. PMID: 30207518 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 12, 2018 Category: Microbiology Authors: Rosteius T, Jansen O, Fehmer T, Baecker H, Citak M, Schildhauer TA, Geßmann J Tags: J Med Microbiol Source Type: research

Conventional culture method and qPCR using 16S rDNA for tissue bank: a comparison using a model of cardiac tissue contamination.
Abstract Real-time polymerase chain reaction (qPCR) using 16S rDNA is an alternative to conventional culture-based tests. The aim of this study was to compare the conventional culture method with qPCR using 16S rDNA in a model of cardiac tissue contamination. Samples of cardiac tissue for artificial contamination with Escherichia coli and control samples were submitted for DNA extraction, which was conducted by selective and alkaline lysis and purification steps. A standard curve for 16S rDNA was constructed to determine the efficiency and analytical sensitivity of the assay in concentrations from 106 to 102&thins...
Source: Journal of Medical Microbiology - September 12, 2018 Category: Microbiology Authors: Tasca Ribeiro VS, Tuon FF, Kraft L, Suss PH, Wollmann LC, Roderjan JG, Brito DA, Alexandrino F, Malgarin JS, Morello LG, da Costa FDA, Pillonetto M Tags: J Med Microbiol Source Type: research

Resistance pattern and distribution of carbapenemase and antiseptic resistance genes among multidrug-resistant Acinetobacter baumannii isolated from intensive care unit patients.
CONCLUSION: Our study revealed the high frequency of multidrug- and carbapenem-resistant isolates of A. baumannii in ICU patients, with a high prevalence of the genes blaOXA-23 and blaOXA-51. However, no apparent biocide resistance was seen in A. baumannii isolates. It appears that appropriate surveillance and control measures are essential to prevent the emergence and transmission of MDR A. baumannii in Iran. PMID: 30175954 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 3, 2018 Category: Microbiology Authors: Shirmohammadlou N, Zeighami H, Haghi F, Kashefieh M Tags: J Med Microbiol Source Type: research

Evaluation of rapid KPC carbapenemase detection method based on MALDI-TOF VITEK MS spectra analysis.
Abstract Clinical microbiology laboratories in hospital settings need to be able to identify patients who carry carbapenemase-producing bacterial strains quickly in order to contain their spread and initiate proper pharmacological therapy. The aim of this study was to confirm the correlation between KPC production and a characteristic mass spectrometry (MS) peak (11 109 Da±8) to validate the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS as a rapid screening tool. With this aim, 176 selected clinical samples that were KPC-producing and 260 control samples that were carbap...
Source: Journal of Medical Microbiology - August 31, 2018 Category: Microbiology Authors: Centonze AR, Bertoncelli A, Savio C, Orza P, Bedenić B, Mazzariol A Tags: J Med Microbiol Source Type: research