Bioarchaeological investigation of individuals with suspected multibacillary leprosy from the mediaeval < em > leprosarium < /em > of St Mary Magdalen, Winchester, Hampshire, UK
Conclusions. The presence of Mycobacterium leprae DNA was confirmed in both females, and genotyping showed SNP type 3I-1 strains but with a clear genotypic variation. We could not confirm Mycobacterium tuberculosis complex DNA in the female individual SK.45. High levels of M. leprae DNA were found within the pulp cavities of four maxillary teeth from the male child (Sk.8) with LO, consistent with the theory that the replication of M. leprae in alveolar bone may interfere with root formation at key stages of development. We report our biomolecular findings in these individuals and review the evidence this site has contribut...
Source: Journal of Medical Microbiology - February 16, 2024 Category: Microbiology Authors: G Michael Taylor Katie White-Iribhogbe Garrard Cole David Ashby Graham R Stewart Heidi Dawson-Hobbis Source Type: research
Genomic and metabolic versatility of < em > Pseudomonas aeruginosa < /em > contributes to its inter-kingdom transmission and survival
J Med Microbiol. 2024 Feb;73(2). doi: 10.1099/jmm.0.001791.ABSTRACTPseudomonas aeruginosa is one of the most versatile bacteria with renowned pathogenicity and extensive drug resistance. The diverse habitats of this bacterium include fresh, saline and drainage waters, soil, moist surfaces, taps, showerheads, pipelines, medical implants, nematodes, insects, plants, animals, birds and humans. The arsenal of virulence factors produced by P. aeruginosa includes pyocyanin, rhamnolipids, siderophores, lytic enzymes, toxins and polysaccharides. All these virulent elements coupled with intrinsic, adaptive and acquired antibiotic r...
Source: Journal of Medical Microbiology - February 16, 2024 Category: Microbiology Authors: Sakthivel Ambreetha Diansy Zincke Dananjeyan Balachandar Kalai Mathee Source Type: research
Comparative evaluation of the detection rate, workflow and associated costs of a multiplex PCR panel versus conventional methods in diagnosis of infectious gastroenteritis
Conclusion. Multiplex PCR panels are valuable tools for laboratory identification of infectious agents causing diarrhoea. The higher costs of such a multiplex PCR panel might be outweighed by the higher detection rate, ease of handling, rapid results and most likely improved patient management. However, these panels do not provide information on antimicrobial susceptibility testing. Therefore, if this is necessary for targeted therapy or if outbreak monitoring and control is required, specimens must still be cultured.PMID:38362908 | DOI:10.1099/jmm.0.001795 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 16, 2024 Category: Microbiology Authors: J Ambrosius-Eichner M Hogardt A Berger G Dultz R Idris V A J Kempf T A Wichelhaus Source Type: research
Bioarchaeological investigation of individuals with suspected multibacillary leprosy from the mediaeval < em > leprosarium < /em > of St Mary Magdalen, Winchester, Hampshire, UK
Conclusions. The presence of Mycobacterium leprae DNA was confirmed in both females, and genotyping showed SNP type 3I-1 strains but with a clear genotypic variation. We could not confirm Mycobacterium tuberculosis complex DNA in the female individual SK.45. High levels of M. leprae DNA were found within the pulp cavities of four maxillary teeth from the male child (Sk.8) with LO, consistent with the theory that the replication of M. leprae in alveolar bone may interfere with root formation at key stages of development. We report our biomolecular findings in these individuals and review the evidence this site has contribut...
Source: Journal of Medical Microbiology - February 16, 2024 Category: Microbiology Authors: G Michael Taylor Katie White-Iribhogbe Garrard Cole David Ashby Graham R Stewart Heidi Dawson-Hobbis Source Type: research
Genomic and metabolic versatility of < em > Pseudomonas aeruginosa < /em > contributes to its inter-kingdom transmission and survival
J Med Microbiol. 2024 Feb;73(2). doi: 10.1099/jmm.0.001791.ABSTRACTPseudomonas aeruginosa is one of the most versatile bacteria with renowned pathogenicity and extensive drug resistance. The diverse habitats of this bacterium include fresh, saline and drainage waters, soil, moist surfaces, taps, showerheads, pipelines, medical implants, nematodes, insects, plants, animals, birds and humans. The arsenal of virulence factors produced by P. aeruginosa includes pyocyanin, rhamnolipids, siderophores, lytic enzymes, toxins and polysaccharides. All these virulent elements coupled with intrinsic, adaptive and acquired antibiotic r...
Source: Journal of Medical Microbiology - February 16, 2024 Category: Microbiology Authors: Sakthivel Ambreetha Diansy Zincke Dananjeyan Balachandar Kalai Mathee Source Type: research
Comparative evaluation of the detection rate, workflow and associated costs of a multiplex PCR panel versus conventional methods in diagnosis of infectious gastroenteritis
Conclusion. Multiplex PCR panels are valuable tools for laboratory identification of infectious agents causing diarrhoea. The higher costs of such a multiplex PCR panel might be outweighed by the higher detection rate, ease of handling, rapid results and most likely improved patient management. However, these panels do not provide information on antimicrobial susceptibility testing. Therefore, if this is necessary for targeted therapy or if outbreak monitoring and control is required, specimens must still be cultured.PMID:38362908 | DOI:10.1099/jmm.0.001795 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 16, 2024 Category: Microbiology Authors: J Ambrosius-Eichner M Hogardt A Berger G Dultz R Idris V A J Kempf T A Wichelhaus Source Type: research
Bioarchaeological investigation of individuals with suspected multibacillary leprosy from the mediaeval < em > leprosarium < /em > of St Mary Magdalen, Winchester, Hampshire, UK
Conclusions. The presence of Mycobacterium leprae DNA was confirmed in both females, and genotyping showed SNP type 3I-1 strains but with a clear genotypic variation. We could not confirm Mycobacterium tuberculosis complex DNA in the female individual SK.45. High levels of M. leprae DNA were found within the pulp cavities of four maxillary teeth from the male child (Sk.8) with LO, consistent with the theory that the replication of M. leprae in alveolar bone may interfere with root formation at key stages of development. We report our biomolecular findings in these individuals and review the evidence this site has contribut...
Source: Journal of Medical Microbiology - February 16, 2024 Category: Microbiology Authors: G Michael Taylor Katie White-Iribhogbe Garrard Cole David Ashby Graham R Stewart Heidi Dawson-Hobbis Source Type: research
< em > STnc1280 < /em > , a trans-coding sRNA is involved in virulence modulation < em > via < /em > targeting < em > gldA < /em > mRNA in < em > Salmonella < /em > Typhimurium
Conclusion. Combining the bioinformatic analysis, these findings suggested that STnc1280 may bind to the SD sequence of gldA mRNA, hindering the binding of ribosomes to gldA mRNA, thereby inhibiting the expression of GldA protein to modulate the virulence of STM.PMID:38353511 | DOI:10.1099/jmm.0.001804 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 14, 2024 Category: Microbiology Authors: Chengcheng Ning Na Li Lixia Wang Yun Guo Chunhui Ji Zhiyuan Li Yunxia Shang Xingxing Zhang Yaoqiang Sun Xiaoxing Huang Qingwen Leng Xuepeng Cai Qingling Meng Jun Qiao Source Type: research
An alternative strategy to increasing influenza virus replication for vaccine production in chicken embryo fibroblast (DF-1) cells by inhibiting interferon alpha and beta using peptide-conjugated phosphorodiamidate morpholino oligomers
Conclusions. This study emphasizes the utility of PPMO in allowing cell cultures to produce increased levels of influenza for vaccine production or alternatively, as a screening tool to cheaply test targets prior to the development of permanent knockouts of host gene expression.PMID:38353513 | DOI:10.1099/jmm.0.001807 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 14, 2024 Category: Microbiology Authors: Meagan A Prescott Hong Moulton Manoj K Pastey Source Type: research
Significant differences in the caecal bacterial microbiota of red and grey squirrels in Britain
Conclusion. Clear differences between the caecal microbiota of the two species of squirrel were identified, which could potentially impact their overall health and ability to compete for resources.PMID:38354038 | DOI:10.1099/jmm.0.001793 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 14, 2024 Category: Microbiology Authors: Lucy Hall Christopher Nichols Francesca Martelli Joy Leng Craig Shuttleworth Roberto La Ragione Source Type: research
< em > STnc1280 < /em > , a trans-coding sRNA is involved in virulence modulation < em > via < /em > targeting < em > gldA < /em > mRNA in < em > Salmonella < /em > Typhimurium
Conclusion. Combining the bioinformatic analysis, these findings suggested that STnc1280 may bind to the SD sequence of gldA mRNA, hindering the binding of ribosomes to gldA mRNA, thereby inhibiting the expression of GldA protein to modulate the virulence of STM.PMID:38353511 | DOI:10.1099/jmm.0.001804 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 14, 2024 Category: Microbiology Authors: Chengcheng Ning Na Li Lixia Wang Yun Guo Chunhui Ji Zhiyuan Li Yunxia Shang Xingxing Zhang Yaoqiang Sun Xiaoxing Huang Qingwen Leng Xuepeng Cai Qingling Meng Jun Qiao Source Type: research
An alternative strategy to increasing influenza virus replication for vaccine production in chicken embryo fibroblast (DF-1) cells by inhibiting interferon alpha and beta using peptide-conjugated phosphorodiamidate morpholino oligomers
Conclusions. This study emphasizes the utility of PPMO in allowing cell cultures to produce increased levels of influenza for vaccine production or alternatively, as a screening tool to cheaply test targets prior to the development of permanent knockouts of host gene expression.PMID:38353513 | DOI:10.1099/jmm.0.001807 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 14, 2024 Category: Microbiology Authors: Meagan A Prescott Hong Moulton Manoj K Pastey Source Type: research
Significant differences in the caecal bacterial microbiota of red and grey squirrels in Britain
Conclusion. Clear differences between the caecal microbiota of the two species of squirrel were identified, which could potentially impact their overall health and ability to compete for resources.PMID:38354038 | DOI:10.1099/jmm.0.001793 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 14, 2024 Category: Microbiology Authors: Lucy Hall Christopher Nichols Francesca Martelli Joy Leng Craig Shuttleworth Roberto La Ragione Source Type: research
Antifungal activity and potential mechanism of action of Huangqin decoction against < em > Trichophyton rubrum < /em >
Conclusion. These results revealed that HQD had significant anti-dermatophyte activity, which was associated with destroying the cell membrane and affecting the enzyme activities of mitochondria.PMID:38348868 | DOI:10.1099/jmm.0.001805 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 13, 2024 Category: Microbiology Authors: Chengying Shen Zhong Luo Ping Zhan Fengyi Deng Pei Zhang Baode Shen Jianxin Hu Source Type: research
Efficacy evaluation of anti-DEC-IgY against antibiotic-resistant diarrhoeagenic < em > Escherichia coli < /em >
Conclusion. The study findings suggest that IgY-based passive immunotherapy could be a potential strategy for managing the risks associated with antibiotic-resistant bacterial infections. Additionally, this study paves the way for the development of IgY-related research and applications in India.PMID:38314672 | DOI:10.1099/jmm.0.001801 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 5, 2024 Category: Microbiology Authors: Srichandrasekar Thuthikkadu Indhuprakash Panchapakesan S David Raj C Diraviyam Thirumalai Source Type: research
