Antimicrobial resistance profiles of Shigella dysenteriae isolated from travellers returning to the UK, 2004-2017.
Abstract PURPOSE: Antimicrobial resistance (AMR) profiles of 754 strains of Shigella dysenteriae isolated between 2004 and 2017 from UK travellers reporting symptoms of gastrointestinal (GI) disease were reviewed to look for evidence of emerging AMR associated with travellers' diarrhoea. METHODOLOGY: A travel history was provided for 72.7 % (548/754) of cases, of which 90.9 % (498/548) reported travel outside the UK within 7 days of onset of symptoms, and 9.1 % (50/498) reported no travel in that time frame. During the course of this study, whole genome sequencing (WGS) was implemented for GI dis...
Source: Journal of Medical Microbiology - June 29, 2018 Category: Microbiology Authors: Terry LM, Barker CR, Day MR, Greig DR, Dallman TJ, Jenkins C Tags: J Med Microbiol Source Type: research

Corrigendum: Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era.
usílek M, Medvecký M, Papagiannitsis CC PMID: 29957176 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 29, 2018 Category: Microbiology Authors: Žemličková H, Mališová L, Španělová P, Jakubů V, Kozáková J, Musílek M, Medvecký M, Papagiannitsis CC Tags: J Med Microbiol Source Type: research

Identification of recombinant coxsackievirus A6 variants in hand, foot and mouth disease in Nanjing, China, 2013.
CONCLUSION: The emerging recombinant CVA6 variants belong to a novel RF-J group which was most likely formed by at least two recombination events. Continued monitoring on the geographical distribution of various CVA6 RFs is essential. PMID: 29947601 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 27, 2018 Category: Microbiology Authors: Qiao M, Yong W, Wang X, Li W, Zhang Z, He M, Shi L, Wang Y, Xie G, Ding J Tags: J Med Microbiol Source Type: research

Bactericidal efficacy of molybdenum oxide nanoparticles against antimicrobial-resistant pathogens.
usa M Abstract Multidrug-resistant bacteria pose a major threat to effective antibiotics and alternatives to fight multidrug-resistant pathogens are needed. We synthetized molybdenum oxide (MoO3) nanoparticles (NP) and determined their antibacterial activity against 39 isolates: (i) eight Staphylococcus aureus, including representatives of methicillin-resistant S. aureus epidemic clones; (ii) six enterococci, including vancomycin-resistant isolates; and (iii) 25 Gram-negative isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterobacter cloacae), including extende...
Source: Journal of Medical Microbiology - June 25, 2018 Category: Microbiology Authors: Lopes E, Piçarra S, Almeida PL, de Lencastre H, Aires-de-Sousa M Tags: J Med Microbiol Source Type: research

The importance of cross-disciplinary research to combat antimicrobial resistance: introducing a new pop-up journal, X-AMR.
PMID: 29932392 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 22, 2018 Category: Microbiology Authors: Knight G, Lambert H, Feil E, Holmes M, Lindsay J Tags: J Med Microbiol Source Type: research

Ecology and diversity in upper respiratory tract microbial population structures from a cross-sectional community swabbing study.
CONCLUSION: The application of epidemiological methods and ecological theory to respiratory tract samples has yielded novel insights into the factors that drive microbial community composition. PMID: 29927372 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Coughtrie AL, Morris DE, Anderson R, Begum N, Cleary DW, Faust SN, Jefferies JM, Kraaijeveld AR, Moore MV, Mullee MA, Roderick PJ, Tuck A, Whittaker RN, Yuen HM, Doncaster CP, Clarke SC Tags: J Med Microbiol Source Type: research

Epidemic spread of OXA-48 beta-lactamase in Croatia.
CONCLUSIONS: The OXA-48-positive organisms found in this study showed wide variability in antibiotic susceptibility, β-lactamase content and PFGE banding patterns. This study revealed a switch from the predominance of VIM-1 in 2012-2013 to that of OXA-48 in the 2015 to 2017. PMID: 29927373 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Bedenić B, Slade M, Starčević LŽ, Sardelić S, Vranić-Ladavac M, Benčić A, Zujić Atalić V, Bogdan M, Bubonja-Šonje M, Tomić-Paradžik M, Tot T, Lukić-Grlić A, Drenjančević D, Varda-Brkić D, Bandić-Pavlović D, Mihaljević S, Zarfel G, Gu Tags: J Med Microbiol Source Type: research

Genetic diversity of pneumococcal surface protein A (PspA) in paediatric isolates of non-conjugate vaccine serotypes in Japan.
CONCLUSION: The present study revealed the predominance of PspA families 1 and 2 in NVTs, and the presence of family 3 in serotype 37. PMID: 29927374 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Kawaguchiya M, Urushibara N, Aung MS, Morimoto S, Ito M, Kudo K, Kobayashi N Tags: J Med Microbiol Source Type: research

Dissemination of newly emerged polymyxin B sensitive Vibrio cholerae O1 containing Haitian-like genetic traits in different parts of India.
CONCLUSION: Polymyxin B-sensitive strains have begun to spread throughout India and may lead to the revision of the biotype classification. The dissemination of these new variant strains needs to be carefully monitored in different endemic populations through active holistic surveillance to understand their clinical and epidemiological consequences. PMID: 29927375 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Samanta P, Saha RN, Chowdhury G, Naha A, Sarkar S, Dutta S, Nandy RK, Okamoto K, Mukhopadhyay AK Tags: J Med Microbiol Source Type: research

Sexually transmitted rectal infections in a cohort of 'men having sex with men'.
CONCLUSIONS: Sexually transmitted rectal infections are frequent and often asymptomatic among MSM. LGV prevalence is high in our country and there is increasing evidence of symptomless cases. PMID: 29927376 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2018 Category: Microbiology Authors: Foschi C, Gaspari V, Sgubbi P, Salvo M, D'Antuono A, Marangoni A Tags: J Med Microbiol Source Type: research

Streptococcus and Prevotella are associated with the prognosis of oesophageal squamous cell carcinoma.
CONCLUSION: Combined Streptococcus and Prevotella abundance is regarded as an independent species prognostic biomarker in ESCC patients. PMID: 29923815 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 20, 2018 Category: Microbiology Authors: Liu Y, Lin Z, Lin Y, Chen Y, Peng XE, He F, Liu S, Yan S, Huang L, Lu W, Xiang Z, Hu Z Tags: J Med Microbiol Source Type: research

Changes in bacterial diversity and composition in the faeces and colon of weaned piglets after feeding fermented soybean meal.
Abstract PURPOSE: The microbiota composition of faeces and colonic contents were analysed to investigate the mechaninsm by which fermented soybean meal improves intestinal microbial communities, growth and immunity in weaning piglets. METHODOLOGY: Microbiota were investigated using16S rRNA gene sequencing and systematical bio-information Operational Taxonomic Units; α-diversity analyses indicated that fermented soybean meal increased bacterial species diversity. RESULTS: The levels of Actinobacteria and Proteobacteia in faeces, and Firmicutes and Tenericutes in the colon, increased significantly in...
Source: Journal of Medical Microbiology - June 20, 2018 Category: Microbiology Authors: Zhu JJ, Gao MX, Song XJ, Zhao L, Li YW, Hao ZH Tags: J Med Microbiol Source Type: research

Outer-membrane protein LptD (PA0595) plays a role in the regulation of alginate synthesis in Pseudomonas aeruginosa.
CONCLUSION: LptD plays a specific role in alginate production. Our findings suggest that there are two pathways for the production of alginate in P. aeruginosa, one involving AlgW in the wild-type, and one involving AlgO in the mucA22 mutant. PMID: 29923820 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 20, 2018 Category: Microbiology Authors: Pandey S, Delgado C, Kumari H, Florez L, Mathee K Tags: J Med Microbiol Source Type: research

The emergence of multi-resistant Enterococcus faecalis clonal complex, CC4, causing nosocomial infections.
CONCLUSIONS: This study demonstrated the prevalent subtypes and resistance profiles of E. faecalis causing nosocomial infection, and indicated that CC4 may be a newly emerging high-risk, multi-resistant cluster. More surveillance is urgently needed, which will increase our understanding of the prevention and treatment of such infections. PMID: 29923823 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 20, 2018 Category: Microbiology Authors: Dai D, Wang H, Xu X, Chen C, Song C, Jiang D, Du P, Zhang Y, Zeng H Tags: J Med Microbiol Source Type: research

In-depth molecular analysis of a small cohort of human and Aedes mosquito (adults and larvae) samples from Kolkata revealed absence of Zika but high prevalence of dengue virus.
CONCLUSION: Both host and vector samples showed absence of ZIKV but high prevalence of DENV. The high rate of infection of larvae with DENV is suggestive of trans-ovarial transmission that could contribute to the surge of human infections during each post-monsoon season. It would be important to guard against false positives using the available Zika-reporting primer sets. PMID: 29897327 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2018 Category: Microbiology Authors: Sukla S, Ghosh A, Saha R, De A, Adhya S, Biswas S Tags: J Med Microbiol Source Type: research

Insights into the genome diversity and virulence of two clinical isolates of Burkholderia cenocepacia.
CONCLUSION: This study mapped existing genetic variations in B. cenocepacia associated with notorious outcomes in CF patients, and the data obtained provide comprehensive, genome-inferred insights and multifactorial examination of an important human pathogen. PMID: 29897328 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2018 Category: Microbiology Authors: Salloum T, Nassour E, Araj GF, Abboud E, Tokajian S Tags: J Med Microbiol Source Type: research

Lactobacillus plantarum ameliorates tumour necrosis factor-induced bacterial translocation in Caco-2 cells by regulation of TLR4 expression.
Abstract PURPOSE: Translocation of bacteria across the intestinal barrier is important in the pathogenesis of systemic sepsis. In inflammatory conditions, commensal bacteria exploit transcytotic pathways to cross the intestinal epithelium in a TLR4-dependent manner. The aim of this study was to test the hypothesis that Lactobacillus plantarum ameliorates tumour necrosis factor-induced bacterial translocation by regulation of Toll-like receptor-4 expression. METHODOLOGY: L. plantarum strains were investigated to determine their capacity to inhibit the initial adhesion of Escherichia coli B5 to Caco-2 cells. Th...
Source: Journal of Medical Microbiology - June 7, 2018 Category: Microbiology Authors: Wang B, Li J, Wang S, Hao Y, Zhao X, Chen J Tags: J Med Microbiol Source Type: research

Emergence and nosocomial spread of ST11 carbapenem-resistant Klebsiella pneumoniae co-producing OXA-48 and KPC-2 in a regional hospital in Taiwan.
CONCLUSION: The clonal spread of K. pneumoniae ST11 expressing OXA-48, KPC-2 and CTX-M-14 β-lactamases was the cause of an outbreak of CRKP. To the best of our knowledge, a single strain harbouring A-, B- and D-class carbapenemase genes has not previously been identified. There is a high prevalence of plasmid-encoded KPC-2- and OXA-48-producing CRKP in our hospital; most isolates were members of ST11, which may be representative of a high-risk CRKP clone disseminating in central Taiwan. PMID: 29873627 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 6, 2018 Category: Microbiology Authors: Chen CM, Guo MK, Ke SC, Lin YP, Li CR, Vy Nguyen HT, Wu LT Tags: J Med Microbiol Source Type: research

High diversity in SCCmec elements among multidrug-resistant Staphylococcus haemolyticus strains originating from paediatric patients; characterization of a new composite island.
CONCLUSION: A high diversity of SCCmec elements with the prevalence of a new composite island was determined among MRSH strains. The structure of the composite island represented by MDR-SH strains in this study, in combination with the presence of a restriction-modification system type III, is described for the first time in this study. The presence of an 8 bp direct repeat (DR) and the sequences flanking the DR may support the integration of the mecA gene complex as a composite transposon (IS431-mecA-IS431) independently from recombinase genes. PMID: 29873628 [PubMed - as supplied by publisher] (Source: Journa...
Source: Journal of Medical Microbiology - June 6, 2018 Category: Microbiology Authors: Hosseinkhani F, Tammes Buirs M, Jabalameli F, Emaneini M, van Leeuwen WB Tags: J Med Microbiol Source Type: research

Detection of toxigenic Clostridium difficile colonization in patients admitted to the hospital for chemotherapy or haematopoietic cell transplantation.
Abstract Increasing evidence suggests that asymptomatic carriers are an important source of healthcare-associated Clostridium difficile infection. However, it is not known which test for the detection of C. difficile colonization is most sensitive in patients with haematological malignancies. We performed a prospective cohort study of 101 patients with haematological malignancies who had been admitted to the hospital for scheduled chemotherapy or haematopoietic cell transplantation. Each patient provided a formed stool sample. We compared the performance of five different commercially available assays, using toxig...
Source: Journal of Medical Microbiology - June 4, 2018 Category: Microbiology Authors: Vaughn JL, Balada-Llasat JM, Lamprecht M, Huang Y, Anghelina M, El Boghdadly Z, Bishop-Hill K, Childs R, Pancholi P, Andritsos LA Tags: J Med Microbiol Source Type: research

Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era.
CONCLUSIONS: WGS revealed specific virulence factors in antibiotic-resistant pneumococcal clones that spread rapidly in the post-vaccine era in the Czech Republic. PMID: 29856703 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 1, 2018 Category: Microbiology Authors: Žemličková H, Mališová L, Španělová P, Jakubů V, Kozáková J, Musílek M, Medvecký M Tags: J Med Microbiol Source Type: research

Antifungal susceptibility and virulence of Candida parapsilosis species complex: an overview of their pathogenic potential.
CONCLUSION: These results reinforce the heterogeneity of these cryptic species for their antifungal susceptibility, virulence and pathogenic potential, emphasizing the relevance of monitoring these emerging pathogens. PMID: 29846153 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 30, 2018 Category: Microbiology Authors: Brilhante RSN, Sales JA, da Silva MLQ, de Oliveira JS, Pereira LA, Pereira-Neto WA, Cordeiro RA, Sidrim JJC, Castelo-Branco DSCM, Rocha MFG Tags: J Med Microbiol Source Type: research

An accidental laboratory exposure to Brucella melitensis: the prospective post-exposure management and a detailed investigation into the nature of the exposure.
Abstract Our aim was to prospectively manage 22 Brucella-exposed individuals and identify the lapses in laboratory practices that lead to the exposure. The exposed individuals were risk-stratified, assessed for post-exposure prophylaxis (PEP), counselled to self-monitor symptoms and followed-up with three serology tests. Staff laboratory practices were recorded. Ten out of 13 high-risk individuals received PEP within 48 h of exposure. Compliance with PEP and serology monitoring was 90 and 96 %, respectively. No brucellosis cases were documented. A single handler manipulated the Brucella isolate on the ope...
Source: Journal of Medical Microbiology - May 30, 2018 Category: Microbiology Authors: Wong C, Ng SY, Tan SH Tags: J Med Microbiol Source Type: research

Tigecycline resistance among Klebsiella pneumoniae isolated from febrile neutropenic patients.
This study emphasizes the importance of growing tigecycline resistance in K. pneumoniae infecting febrile neutropenic patients. Concerning the mechanism of resistance, it was clear that the ramA gene plays the major role, although alternative resistance mechanisms may also exist. PMID: 29799385 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 25, 2018 Category: Microbiology Authors: Elgendy SG, Abdel Hameed MR, El-Mokhtar MA Tags: J Med Microbiol Source Type: research

Stenotrophomonas maltophilia isolated from patients exposed to invasive devices in a university hospital in Argentina: molecular typing, susceptibility and detection of potential virulence factors.
CONCLUSION: This is the first epidemiological study of the clonal relatedness of S. maltophilia clinical isolates in Argentina. Great genomic diversity was observed, and only two small clusters of related S. maltophilia types were found. Minocycline and trimethoprim-sulfamethoxazole were the most active agents. S. maltophilia virulence in the G. mellonella model is multifactorial, and further studies are needed to elucidate the role of each potential virulence factor. PMID: 29799387 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 25, 2018 Category: Microbiology Authors: Alcaraz E, Garcia C, Papalia M, Vay C, Friedman L, Passerini de Rossi B Tags: J Med Microbiol Source Type: research

Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece.
Abstract Extended-spectrum cephalosporin- and/or carbapenem-resistant (ESCR and/or CarbR) Enterobacteriaceae constitute a public health hazard because of limited treatment options and are endemic among humans in Greece. Recently, ESCR and CarbREnterobacteriaceae have been increasingly isolated from companion animals, stressing their potential role as a reservoir for humans. However, the presence of ESCR bacteria in companion animals within Greek households has not been determined yet. Genes conferring the ESCR and CarbR phenotype were detected among canine isolates and their chromosomal or plasmid location was det...
Source: Journal of Medical Microbiology - May 25, 2018 Category: Microbiology Authors: Liakopoulos A, Betts J, La Ragione R, van Essen-Zandbergen A, Ceccarelli D, Petinaki E, Koutinas CK, Mevius DJ Tags: J Med Microbiol Source Type: research

Effect of respiratory Achromobacter spp. infection on pulmonary function in patients with cystic fibrosis.
CONCLUSIONS: The Achromobacter spp. status in CF shows a trend towards more severe airflow obstruction and an association with accelerated decline in lung function parameters. PMID: 29787364 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 22, 2018 Category: Microbiology Authors: Recio R, Brañas P, Martínez MT, Chaves F, Orellana MA Tags: J Med Microbiol Source Type: research

KPC-2 producing ST101 Klebsiella pneumoniae from bloodstream infection in India.
This study characterizes KPC-2 producing Klebsiella pneumoniae belonging to ST101. Whole genome sequencing using the Ion Torrent PGM platform with 400 bp chemistry was performed. blaKPC-2 was found on an IncFIIK plasmid associated with ISKpn6 and ISKpn7 without Tn4401. This is the first report of KPC-2 K. pneumoniae from bacteremia in India. The isolate also coded for other resistance genes such as aadA1, aadA2, armA, aac(3)-Ild, aac(6')-Ild for aminoglycoside; blaSHV-11, blaTEM-1B, blaOXA-9, for β-lactams and aac(6')-Ild, oqxA, oqxB, qnrB1 for fluoroquinolones. It belonged to the K17 capsular type. India is en...
Source: Journal of Medical Microbiology - May 22, 2018 Category: Microbiology Authors: Shankar C, Shankar BA, Manesh A, Veeraraghavan B Tags: J Med Microbiol Source Type: research

Methicillin-resistant Staphylococcus argenteus misidentified as methicillin-resistant Staphylococcus aureus emerging in western Sweden.
Svensson Stadler L Abstract Two strains included in a whole-genome sequencing project for methicillin-resistant Staphylococcus aureus (MRSA) were identified as non-Staphylococcus aureus when the sequences were analysed using the bioinformatics software ALEX (www.1928diagnostics.com, Gothenburg, Sweden). Sequencing of the sodA gene of these strains identified them as Staphylococcus argenteus. The collection of MRSA in western Sweden was checked for additional strains of this species. A total of 18 strains of S. argenteus isolated between 2011 and December 2017 were identified. PMID: 29771232 [PubMed - as suppl...
Source: Journal of Medical Microbiology - May 17, 2018 Category: Microbiology Authors: Tång Hallbäck E, Karami N, Adlerberth I, Cardew S, Ohlén M, Engström Jakobsson H, Svensson Stadler L Tags: J Med Microbiol Source Type: research

Regulation of gene expression of hcp, a core gene of the type VI secretion system in Acinetobacter baumannii causing respiratory tract infection.
CONCLUSION: The type VI secretion system and hcp predominate in A. baumannii causing respiratory infections. Expression of hcp is regulated by the infection status and acid environment, and might play a role in the process of triggering infection by the colonizer. PMID: 29771233 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 17, 2018 Category: Microbiology Authors: Hu YY, Liu CX, Liu P, Wu ZY, Zhang YD, Xiong XS, Li XY Tags: J Med Microbiol Source Type: research

Can dalbavancin be used as a catheter lock solution?
CONCLUSIONS: Dalbavancin proved to be active against MRSA and MRSE biofilms. The combination of dalbavancin with heparin is a promising catheter lock solution that has the advantage of locking the catheter at home for 7 days. PMID: 29771236 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 17, 2018 Category: Microbiology Authors: Díaz-Ruíz C, Alonso B, Cercenado E, Cruces R, Bouza E, Muñoz P, Guembe M Tags: J Med Microbiol Source Type: research

Relevance of antifungal penetration in biofilm-associated resistance of Candida albicans and non-albicans Candida species.
This study aimed to determine the relevance of penetration limitation in the antifungal resistance of biofilms formed by C. albicans and NAC clinical isolates, using an agar disk diffusion assay. The penetration of posaconazole and amphotericin B through the biofilms was significantly reduced. Fluconazole, voriconazole and caspofungin showed a superior penetration capacity in C. albicans, Candida tropicalis and Candida parapsilosis biofilms, but exhibited inter-species and strain/isolate variation. Candida krusei biofilms were the most resilient to antifungal permeation. All of the antifungal drugs failed to kill the biofi...
Source: Journal of Medical Microbiology - May 16, 2018 Category: Microbiology Authors: Singh R, Kumari A, Kaur K, Sethi P, Chakrabarti A Tags: J Med Microbiol Source Type: research

Nalidixic acid surrogate test for susceptibility to ciprofloxacin in Salmonella. Revisiting the question.
Gutiérrez-Fernández J Abstract We investigated the reliability of nalidixic acid (NA) susceptibility as a marker of ciprofloxacin susceptibility in Salmonella, analysing 302 stool isolates. NC53 of the MicroScan system was used for NA susceptibility tests and the E-test was used for ciprofloxacin susceptibility tests. Among the isolates, 178 (58.9 %) were serogroup B, 74 (24.5 %) were serogroup D, 27 (8.9 %) were serogroup C and 23 (7.6 %) were from other minor serogroups. Globally, susceptibility to NA correctly predicted the susceptibility of Salmonella to ciprofloxacin, with a sensitivity ...
Source: Journal of Medical Microbiology - May 16, 2018 Category: Microbiology Authors: Guzmán-Martín JL, Navarro-Marí JM, Expósito-Ruiz M, Gutiérrez-Fernández J Tags: J Med Microbiol Source Type: research

Identification of Acinetobacter seifertii isolated from Bolivian hospitals.
We present here the case of three A. seifertii clinical isolates which were initially identified as Acinetobacter sp. by phenotypic methods but no identification at the species level was achieved using semi-automated identification methods. The isolates were further analysed by whole genome sequencing and identified as A. seifertii. Due to the fact that A. seifertii has been isolated from serious infections such as respiratory tract and bloodstream infections, we emphasize the importance of correctly identifying isolates of the genus Acinetobacter at the species level to gain a deeper knowledge of their prevalence and clin...
Source: Journal of Medical Microbiology - May 8, 2018 Category: Microbiology Authors: Cerezales M, Xanthopoulou K, Ertel J, Nemec A, Bustamante Z, Seifert H, Gallego L, Higgins PG Tags: J Med Microbiol Source Type: research

Identification and characterization of a novel single-chain variable fragment (scFv) antibody against Neisseria meningitidis factor H-binding protein (fHbp).
CONCLUSION: The affinity of the selected scFv was ~8.65×109 M-1. The isolated scFv can provide the basis for developing a diagnostic kit. PMID: 29737965 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 8, 2018 Category: Microbiology Authors: Yarian F, Kazemi B, Bandehpour M Tags: J Med Microbiol Source Type: research

Demographic, clinical and laboratory features of leptospirosis and dengue co-infection in Malaysia.
CONCLUSION: There is a considerable prevalence of LDCI in this country of which overlapping demographic, clinical and laboratory presentations pose diagnostic and therapeutic challenges. Efforts to raise awareness regarding LDCI, better access to diagnostic services and further prospective studies are warranted. PMID: 29724267 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 4, 2018 Category: Microbiology Authors: Hishamshah M, Ahmad N, Mohd Ibrahim H, Nur Halim NA, Nawi S, Amran F Tags: J Med Microbiol Source Type: research

Validation of a plasma metabolomics model that allows anticipation of the occurrence of cytomegalovirus DNAaemia in allogeneic stem cell transplant recipients.
e;ana JL, Solano C, Navarro D Abstract A plasma metabolomic model obtained by means of untargeted 1H nuclear magnetic resonance, to which taurine, choline, methylamine, total glutathione, trimethylamine N-oxide, lactate, lysine, isoleucine, total fatty acids and unsaturated fatty acids contributed, was validated for the prediction of first episodes of cytomegalovirus (CMV) DNAaemia in a cohort of 79 allogeneic stem haematopoietic stem cell transplant (allo-HSCT) recipients. The predictive success rate was nearly 65 % for patients at both low and high risk of CMV-related complications according to their baseline ...
Source: Journal of Medical Microbiology - May 4, 2018 Category: Microbiology Authors: Monleón D, Talaya A, Giménez E, Vinuesa V, Morales JM, Hernández-Boluda JC, Pérez A, Piñana JL, Solano C, Navarro D Tags: J Med Microbiol Source Type: research

Enterotoxin gene profile of methicillin-resistant Staphylococcus pseudintermedius isolates from dogs, humans and the environment.
CONCLUSION: We show that isolates of MRSP vary in their virulence gene profiles, depending on the source from which they have been isolated. This insight should encourage the development of appropriate monitoring and mitigation strategies to reduce the transmission of MRSP in veterinary hospitals and households. PMID: 29724270 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 4, 2018 Category: Microbiology Authors: Phumthanakorn N, Fungwithaya P, Chanchaithong P, Prapasarakul N Tags: J Med Microbiol Source Type: research

Performance of OMNIgene •SPUTUM (DNA Genotek) and cetylpyridinium chloride for sputum storage prior to mycobacterial culture.
Performance of OMNIgene•SPUTUM (DNA Genotek) and cetylpyridinium chloride for sputum storage prior to mycobacterial culture. J Med Microbiol. 2018 May 02;: Authors: Affolabi D, Sanoussi N, Sossou A, Nys T, Bodi O, Esse M, Houeto S, Massou F, de Jong BC, Rigouts L Abstract PURPOSE: The aim was to assess the performance of both cetylpyridinium chloride (CPC) and OMNIgene•SPUTUM (OMNI) reagents for the maintenance of Mycobacterium tuberculosis viability in sputum prior to recovery by culture. METHODOLOGY: Using 312 sputa, we evaluated the performance of the two reagents using culture on L&...
Source: Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Affolabi D, Sanoussi N, Sossou A, Nys T, Bodi O, Esse M, Houeto S, Massou F, de Jong BC, Rigouts L Tags: J Med Microbiol Source Type: research

Role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of various Candida species.
Abstract The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix carbohydrates, proteins and e-DNA were quantified by phenol-sulfuric acid, bicinchoninic acid and UV spectroscopy, respectively. Specific glycosyl residues were detected by dot blot. The cell-surface hydrophobicity was determined by hydrocarbon adhesion assay. Candida tropicalis was found to ...
Source: Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Kumari A, Mankotia S, Chaubey B, Luthra M, Singh R Tags: J Med Microbiol Source Type: research

Contribution of efflux to colistin heteroresistance in a multidrug resistant Acinetobacter baumannii clinical isolate.
CONCLUSION: This study shows that efflux activity contributes to colistin heteroresistance in an MDR A. baumannii clinical isolate. The use of efflux inhibitors as adjuvants of the therapy can resensitize A. baumannii to colistin and prevent the emergence of drug resistance. PMID: 29717972 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Machado D, Antunes J, Simões A, Perdigão J, Couto I, McCusker M, Martins M, Portugal I, Pacheco T, Batista J, Toscano C, Viveiros M Tags: J Med Microbiol Source Type: research

Surveillance of antimicrobial resistance in Neisseria meningitidis strains isolated from invasive cases in Brazil from 2009 to 2016.
Abstract PURPOSE: To describe the antimicrobial resistance profile of Neisseria meningitidis isolates causing invasive disease in Brazil from 2009 to 2016. METHODOLOGY: Among 3548 N. meningitidis isolates received, 2888 (81.4 %) were analysed for antimicrobial resistance using the broth microdilution technique, as recommended by the Clinical and Laboratory Standards Institute. Isolates were tested for ciprofloxacin, chloramphenicol, ceftriaxone, penicillin G, ampicillin and rifampin. RESULTS: All the isolates tested were susceptible to ceftriaxone, while 953 (33.0 %), 1307 (45.3 %) and 2 (0.07 %)...
Source: Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Gorla MC, Pinhata JMW, Dias UJ, de Moraes C, Lemos AP Tags: J Med Microbiol Source Type: research

Abundance of enterovirus C in RD-L20B cell culture-negative stool samples from acute flaccid paralysis cases in Nigeria is geographically defined.
CONCLUSION: The results of this study showed that the presence of EVs and consequently EV-Cs in AFP samples declared to be EV-free by the RD-L20B cell culture-based algorithm varies by geographical region in Nigeria. PMID: 29708482 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 30, 2018 Category: Microbiology Authors: Donbraye E, Olasunkanmi OI, Opabode BA, Ishola TR, Faleye TOC, Adewumi OM, Adeniji JA Tags: J Med Microbiol Source Type: research

Phylogenetic comparison of enteroinvasive Escherichia coli isolated from cases of diarrhoeal disease in England, 2005-2016.
Abstract We compared the genomes of 60 isolates of enteroinvasive Escherichia coli (EIEC) in order to better understand the step-wise evolutionary process from non-pathogenic to host-adapted pathogenic E. coli. All isolates belonged to either sequence type (ST) 6, ST99 or ST270. Each ST was located on different branches of the E. coli phylogeny and had invasion plasmids (pINVs) belonging to FII-21 (ST99, ST270), FII-27 (ST270) or FII-28 (ST6, ST270) incompatibility groups. A higher number of insertion sequence (IS) elements were identified in ST6 and ST270 than in ST99, and appeared to be driving the loss of funct...
Source: Journal of Medical Microbiology - April 25, 2018 Category: Microbiology Authors: Cowley LA, Oresegun DR, Chattaway MA, Dallman TJ, Jenkins C Tags: J Med Microbiol Source Type: research

Urinary tract infection by Acinetobacter baumannii and Pseudomonas aeruginosa: evolution of antimicrobial resistance and therapeutic alternatives.
CONCLUSION: In our setting, it is no longer possible to use carbapenems and piperacillin-tazobactam for empirical treatment of UTI due to A. baumannii or to use piperacillin-tazobactam for empirical treatment of UTI due to P. aeruginosa. Colistin was found to be the most active antibiotic in vitro. There was a marked increase in the consumption of piperacillin-tazobactam. PMID: 29693543 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 25, 2018 Category: Microbiology Authors: Jiménez-Guerra G, Heras-Cañas V, Gutiérrez-Soto M, Del Pilar Aznarte-Padial M, Expósito-Ruiz M, Navarro-Marí JM, Gutiérrez-Fernández J Tags: J Med Microbiol Source Type: research

A case of haemolytic uraemic syndrome (HUS) revealed an outbreak of Shiga toxin-2-producing Escherichia coli O26:H11 infection in a nursery, with long-lasting shedders and person-to-person transmission, Italy 2015.
CONCLUSIONS: This study confirms that Stx2-producing E. coli O26 represents an emerging public health problem. The occurrence of outbreaks of infection by Stx2-producing E. coli O26 in nurseries is of particular concern, given the high probability of infection progressing in severity and resulting in secondary cases. PMID: 29687765 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 23, 2018 Category: Microbiology Authors: Scavia G, Gianviti A, Labriola V, Chiani P, Maugliani A, Michelacci V, Minelli F, Tozzoli R, Caprioli A, Morabito S Tags: J Med Microbiol Source Type: research

Identification and prevalence of RND family multidrug efflux pump oqxAB genes in Enterococci isolates from swine manure in China.
CONCLUSION: This is the first time that the oqxAB genes have been identified in Enterococcus faecalis from swine manure. The genetic linkage of oqxAB-aph(3')-IIa in Enterococcus has not been described before. The high prevalence of oqxAB genes in Enterococcus suggests that it may constitute a reservoir for oqxAB genes and pose a potential threat to public health. PMID: 29687766 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 23, 2018 Category: Microbiology Authors: Yuan L, Zhai YJ, Wu H, Sun HR, He ZP, Wang YB, Pan YS, Kuang NN, Hu GZ Tags: J Med Microbiol Source Type: research

Growth characteristics of liquid cultures increase the reliability of presumptive identification of Mycobacterium tuberculosis complex.
In conclusion, the growth characteristics of mycobacteria in MGIT, when considered along with cord formation, increased the reliability of the presumptive identification, which has a great impact on the laboratory budget and turnaround times. PMID: 29687767 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 23, 2018 Category: Microbiology Authors: Pinhata JMW, Felippe IM, Gallo JF, Chimara E, Ferrazoli L, de Oliveira RS Tags: J Med Microbiol Source Type: research

Diagnostics and antibiotic therapy for spondylodiscitis.
The objective of this retrospective study was to characterise these patients and identify factors for a favourable healing process - with an emphasis on antibiotic therapy. METHODOLOGY: Findings/treatment regimens for all patients with spondylodiscitis treated at a tertiary care hospital in 2012/2013 were recorded. The patients were classified into two groups, depending on the healing process. RESULTS: Fifty-seven patients met the clinical/radiological criteria for spondylodiscitis. The main symptom was massive back pain, combined with a moderate CRP elevation (median 96 mg l-1), and occasionally fever (39 %). ...
Source: Journal of Medical Microbiology - April 23, 2018 Category: Microbiology Authors: Lestin-Bernstein F, Tietke M, Briedigkeit L, Heese O Tags: J Med Microbiol Source Type: research

Characterization of SCCmec type IV methicillin-resistant Staphylococcus aureus clones increased in Japanese hospitals.
Abstract Recently, the prevalence of staphylococcal cassette chromosome mec (SCCmec) type IV isolates, which are the major community-acquired methicillin-resistant Staphylococcus aureus (MRSA), have increased in Japanese hospitals. The aim of this study was to elucidate the detailed molecular epidemiological features of the SCCmec type IV clones in Japanese hospitals. When 2589 MRSA isolated from four hospitals in Tokyo, Japan between 2010 and 2014 were analysed, the proportion of SCCmec type IV overtook that of type II, which was the major type of hospital-acquired MRSA in 2014. Multilocus sequence typing showed ...
Source: Journal of Medical Microbiology - April 20, 2018 Category: Microbiology Authors: Nakaminami H, Takadama S, Ito A, Hasegawa M, Jono C, Noguchi M, Shoshi M, Wajima T, Fujii T, Maruyama H, Sakamoto H, Ito Y, Okamoto S, Masaki Y, Tsuchiya K, Nishinarita S, Noguchi N Tags: J Med Microbiol Source Type: research