Cluster-distinguishing genotypic and phenotypic diversity of carbapenem-resistant Gram-negative bacteria in solid-organ transplantation patients: a comparative study.
Abstract PURPOSE: Solid-organ transplant recipients may display high rates of colonization and/or infection by multidrug-resistant bacteria. We analysed and compared the phenotypic and genotypic diversity of carbapenem-resistant (CR) strains of Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii isolated from patients in the Solid Organ Transplantation department of our hospital. METHODOLOGY: Between March 2012 and August 2013, 56 CR strains from various biological fluids underwent antimicrobial susceptibility testing with VITEK 2, molecular analysis by PCR amplification and genotypic an...
Source: Journal of Medical Microbiology - July 31, 2017 Category: Microbiology Authors: Karampatakis T, Geladari A, Politi L, Antachopoulos C, Iosifidis E, Tsiatsiou O, Karyoti A, Papanikolaou V, Tsakris A, Roilides E Tags: J Med Microbiol Source Type: research

Novel adenoviruses detected in British mustelids, including a unique Aviadenovirus in the tissues of pine martens (Martes martes).
Abstract Several adenoviruses are known to cause severe disease in veterinary species. Recent evidence suggests that canine adenovirus type 1 (CAV-1) persists in the tissues of healthy red foxes (Vulpes vulpes), which may be a source of infection for susceptible species. It was hypothesized that mustelids native to the UK, including pine martens (Martes martes) and Eurasian otters (Lutra lutra), may also be persistently infected with adenoviruses. Based on high-throughput sequencing and additional Sanger sequencing, a novel Aviadenovirus, tentatively named marten adenovirus type 1 (MAdV-1), was detected in pine ma...
Source: Journal of Medical Microbiology - July 28, 2017 Category: Microbiology Authors: Walker D, Gregory WF, Turnbull D, Rocchi M, Meredith AL, Philbey AW, Sharp CP Tags: J Med Microbiol Source Type: research

In vitro efficacy of 16 antimicrobial drugs against a large collection of β-lactamase-producing isolates of extraintestinal pathogenic Escherichia coli from dogs and cats.
CONCLUSION: This study demonstrates that the susceptibility rates to PTZ, CMZ, LMX, AMK, FOM, FPM, NFT and FMX were similar to those to carbapenems (>90 %), implying that these drugs are available alternatives to carbapenems for the treatment of companion animals infected with ExPEC-producing CTX-M-type ESBLs. Further in vivo studies of the effective use of these antimicrobials are required. PMID: 28749329 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 28, 2017 Category: Microbiology Authors: Shimizu T, Harada K, Tsuyuki Y, Kimura Y, Miyamoto T, Hatoya S, Hikasa Y Tags: J Med Microbiol Source Type: research

In vitro activity of bedaquiline against rapidly growing nontuberculous mycobacteria.
The objective of this study was to evaluate the in vitro activity of BDQ against rapidly growing mycobacteria by assessing the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against 18 NTM strains. For MIC determination we performed the resazurin microtitre assay broth dilution, and for the MBC the c.f.u. was determined. BDQ exhibited a strong inhibitory effect against most NTM tested; however, for some NTM strains the MBC was significantly higher than the MIC. A new finding is that Mycobacterium flavescens has a mutation in the gene atpE associated with natural resistance to BDQ. These...
Source: Journal of Medical Microbiology - July 28, 2017 Category: Microbiology Authors: Aguilar-Ayala DA, Cnockaert M, André E, Andries K, Gonzalez-Y-Merchand JA, Vandamme P, Palomino JC, Martin A Tags: J Med Microbiol Source Type: research

High prevalence of multidrug-resistant Escherichia coli isolates from children with and without diarrhoea and their susceptibility to the antibacterial activity of extracts/fractions of fruits native to Mexico.
CONCLUSION: A high number of antimicrobial-resistant E. coli (especially to β-lactams and sulfonamides) and MDR isolates were detected in children under 5 years old, irrespective of diarrhoea symptoms; this is novel information for Culiacan, Sinaloa, Mexico. Moreover, our results showed that the studied fruit extracts/fractions are potential alternative or complementary treatments for MDR E. coli strains. PMID: 28742000 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 26, 2017 Category: Microbiology Authors: Uribe-Beltrán MJ, Ahumada-Santos YP, Díaz-Camacho SP, Eslava-Campos CA, Reyes-Valenzuela JE, Báez-Flores ME, Osuna-Ramírez I, Delgado-Vargas F Tags: J Med Microbiol Source Type: research

Retention of virulence following colistin adaptation in Klebsiella pneumoniae is strain-dependent rather than associated with specific mutations.
This study aimed to understand the impact on virulence and fitness of mutations in specific genes found after adaptation of Klebsiella pneumoniae to colistin. Isolates with an increase in their inhibitory concentration (MIC) to colistin of 32- to>128-fold were shown to have mutations in mgrB, phoPQ and pmrAB, all known regulators of pathways affecting membrane lipid content. When these strains were used in studies in Galleria mellonella there was no clear correlation between mutations in specific genes per se and loss of virulence. Strains which showed sequence duplication in the HAMP-domain of PmrB showed reduced virul...
Source: Journal of Medical Microbiology - July 21, 2017 Category: Microbiology Authors: Wand ME, Bock LJ, Sutton JM Tags: J Med Microbiol Source Type: research

Evolutionary changes in the capsid P2 region of Australian strains of the norovirus GII.Pe_GII.4.
CONCLUSION: The current study helps establish the validity of previously proposed hypervariable sites in the P2 region as well as indicating new ones. It also provides quantitative and qualitative data on how these sites changed over the evolutionary history of a particular norovirus strain. PMID: 28742004 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 21, 2017 Category: Microbiology Authors: Bruggink LD, Moselen JM, Roberts JA, Marshall JA Tags: J Med Microbiol Source Type: research

Combination of polymyxin B and meropenem eradicates persister cells from Acinetobacter baumannii strains in exponential growth.
PMID: 28721845 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 18, 2017 Category: Microbiology Authors: Gallo SW, Ferreira CAS, de Oliveira SD Tags: J Med Microbiol Source Type: research

Evaluation of biofilm-specific antimicrobial resistance genes in Pseudomonas aeruginosa isolates in Farabi Hospital.
CONCLUSIONS: Our results showed a considerable ability of biofilm production, as well as the occurrence of biofilm-specific antimicrobial resistance genes (ndvB and tssC1), in P. aeruginosa isolates from ocular infections in Farabi Hospital. PMID: 28721848 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 18, 2017 Category: Microbiology Authors: Saffari M, Karami S, Firoozeh F, Sehat M Tags: J Med Microbiol Source Type: research

Effective protection of mice against Shigella flexneri with a new self-adjuvant multicomponent vaccine.
CONCLUSION: The convenient and safe manufacturing process, and the preliminary biological evaluation, support the use of the self-adjuvanted HT complex as a new vaccine candidate to face shigellosis. Further development is required, such as additional immune analyses, to evaluate whether this new subunit vaccine can be useful in achieving full protection against Shigella. PMID: 28721849 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 18, 2017 Category: Microbiology Authors: Pastor Y, Camacho A, Gil AG, Ramos R, Ceráin AL, Peñuelas I, Irache JM, Gamazo C Tags: J Med Microbiol Source Type: research

Development and evaluation of a multiple-locus variable-number tandem-repeats analysis assay for subtyping Salmonella Typhi strains from sub-Saharan Africa.
CONCLUSION: The MLVA assay presented in this study is a simple, rapid and more accessible tool that serves as a good alternative to other molecular subtyping methods for S. Typhi. PMID: 28721851 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 18, 2017 Category: Microbiology Authors: Tau NP, Smith AM, Wain JR, Tarupiwa A, Coulibaly KJ, Keddy KH, Germs-Sa Tags: J Med Microbiol Source Type: research

Cell surface physiology and outer cell envelope impermeability for hydrophobic substances in Burkholderia multivorans.
CONCLUSION: These data support the conclusion that EPS expression and the consistent mucoid phenotype are not necessarily associated with the ability of the outer cell surface to associate with non-polar substances or cellular capsulation. PMID: 28721855 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 18, 2017 Category: Microbiology Authors: Ruskoski SA, Champlin FR Tags: J Med Microbiol Source Type: research

Quantitative and structural analyses of the in vitro and ex vivo biofilm-forming ability of dermatophytes.
CONCLUSION: This study sheds light on biofilms of different dermatophyte species, which will contribute to a better understanding of the pathophysiology of dermatophytosis. Further studies of this type are necessary to investigate the processes involved in the formation and composition of dermatophyte biofilms. PMID: 28708048 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 15, 2017 Category: Microbiology Authors: Brilhante RSN, Correia EEM, Guedes GMM, Pereira VS, Oliveira JS, Bandeira SP, Alencar LP, Andrade ARC, Castelo-Branco DSCM, Cordeiro RA, Pinheiro AQ, Chaves LJQ, Pereira Neto WA, Sidrim JJC, Rocha MFG Tags: J Med Microbiol Source Type: research

Hyperinvasiveness and increased intercellular spread of Listeria monocytogenes sequence type 1 are independent of listeriolysin S, internalin F and internalin J1.
CONCLUSION: Our results underline the importance of using relevant clinical strains when investigating L. monocytogenes virulence. We show that despite the association with CC1, llsB, inlF and inlJ1 are not involved in the hyperinvasiveness and efficient intercellular spread of ST1 in various cell types. PMID: 28708050 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 15, 2017 Category: Microbiology Authors: Rupp S, Bärtschi M, Frey J, Oevermann A Tags: J Med Microbiol Source Type: research

Rapid diagnosis of extrapulmonary tuberculosis with Xpert Mycobacterium tuberculosis/rifampicin assay.
CONCLUSION: Compared with AFB smear and solid culture, Xpert MTB/RIF assay has high sensitivities and short detection time, so could be used as an alternative for the rapid diagnosis of EPTB and rifampin resistance in clinical practice. PMID: 28708053 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 15, 2017 Category: Microbiology Authors: Li Y, Pang Y, Zhang T, Xian X, Wang X, Yang J, Wang R, Chen M, Chen W Tags: J Med Microbiol Source Type: research

A high prevalence of beak and feather disease virus in & nbsp;non-psittacine Australian birds.
CONCLUSION: Further research to determine the infectivity and transmissibility of BFDV in non-psittacine species is indicated. Until such work is undertaken the findings from this study suggest that every bird should be considered a potential carrier of BFDV, regardless of species and clinical presentation. Veterinary clinics and wildlife rehabilitation facilities caring for birds that are susceptible to PBFD should reconsider biosecurity protocols aimed at controlling BFDV. PMID: 28703699 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 13, 2017 Category: Microbiology Authors: Amery-Gale J, Marenda MS, Owens J, Eden PA, Browning GF, Devlin JM Tags: J Med Microbiol Source Type: research

Comparative study of isolates from community-acquired and catheter-associated urinary tract infections with reference to biofilm-producing property, antibiotic sensitivity and multi-drug resistance.
CONCLUSIONS: BPP was found in both Com-UTI and CAUTI. When used together, the Safranin tube test and the CRA test increased the sensitivity of detecting BPP. MDR was higher in CAUTI than Com-UTI. MDR and BPP are not interrelated or associated, especially in settings where it is not certain that isolates were obtained from a well-formed biofilm. However, this does not rule out a higher incidence or prevalence of MDR in isolates with BPP taken directly from the biofilms. PMID: 28703700 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 13, 2017 Category: Microbiology Authors: Bardoloi V, Yogeesha Babu KV Tags: J Med Microbiol Source Type: research

In vitro synergistic effects of a short cationic peptide and clinically used antibiotics against drug-resistant isolates of Brucella melitensis.
Abstract PURPOSE: In the last few decades, increasing microbial resistance to common antibiotics has attracted researchers' attention to the development of new classes of antibiotics such as antimicrobial peptides. Accordingly, the aim of the current study was to evaluate antimicrobial effects of the CM11 peptide alone and combined with common antibiotics against drug-resistant isolates of Brucella melitensis. METHODOLOGY: A total of 50 pathogenic samples of B. melitensis were isolated from patients and their antibiotic susceptibility pattern was evaluated by E-test. Then, the synergistic reaction of the pept...
Source: Journal of Medical Microbiology - July 12, 2017 Category: Microbiology Authors: Mohammadi Azad Z, Moravej H, Fasihi-Ramandi M, Masjedian F, Nazari R, Mirnejad R, Moosazadeh Moghaddam M Tags: J Med Microbiol Source Type: research

Chemical composition and cytotoxicity of extracts of marjoram and rosemary and their activity against Sporothrix brasiliensis.
CONCLUSION: These findings support the potential usefulness of the HAE of marjoram in the treatment of sporotrichosis. PMID: 28699881 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 12, 2017 Category: Microbiology Authors: Waller SB, Madrid IM, Hoffmann JF, Picoli T, Cleff MB, Chaves FC, Faria RO, Meireles MCA, Braga de Mello JR Tags: J Med Microbiol Source Type: research

Is SpxA2 involved in hydrogen peroxide production and competence development in Streptococcus sanguinis?
CONCLUSION: Although the intricate relationship between SpxA2, pox-mediated H2O2 production and competence development was clarified in S. sanguinis, it would be worthwhile to explore further whether H2O2 is involved in competence development through oxidizing the SpxA2 CXXC motif. PMID: 28693663 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 10, 2017 Category: Microbiology Authors: Li T, Xu M, Zheng L Tags: J Med Microbiol Source Type: research

Characterization of high level ampicillin- and aminoglycoside-resistant enterococci isolated from non-hospital sources.
CONCLUSION: This is the first report on the characterization of high level ampicillin- and aminoglycoside-resistant Enterococcus faecium among animals and vegetables in Nigeria. The results show that non-hospital sources can constitute a reservoir for potential dissemination of these strains and genes to humans via the food chain or by direct contact. PMID: 28693665 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 10, 2017 Category: Microbiology Authors: Ngbede EO, Raji MA, Kwanashie CN, Kwaga JKP, Adikwu AA, Maurice NA, Adamu AM Tags: J Med Microbiol Source Type: research

Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay.
CONCLUSION: We conclude that the rFC-based assay is a good replacement for conventional LAL assays and as it correlates significantly with IL-6 produced by a human monocyte cell line it could potentially be more useful for detecting LPS in a clinical setting. PMID: 28693666 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 10, 2017 Category: Microbiology Authors: Abate W, Sattar AA, Liu J, Conway ME, Jackson SK Tags: J Med Microbiol Source Type: research

Distribution of Chlamydia trachomatis genotypes in neonatal conjunctivitis in Hungary.
The objective of the present study was to determine the frequency and age distribution of different Chlamydia trachomatis (CT) genotypes causing ophthalmia neonatorum (ON) in Hungary. Using CT specific PCR, we tested 76 conjunctival samples from symptomatic infants up to 3 months old in the National Centre for Epidemiology, Budapest between 2008 and 2016. CT tested positive in 30 of 76 conjunctival samples (39.5 %). The sequencing of the positive samples was successful in every case but one, and resulted in 48 % dominance for genotype E (14/29), followed by 24 % for genotype G (7/29), 10 % for J (3/29), 6.9 % for...
Source: Journal of Medical Microbiology - July 10, 2017 Category: Microbiology Authors: Balla E, Petrovay F, Erdősi T, Balázs A, Henczkó J, Urbán E, Donders GGG Tags: J Med Microbiol Source Type: research

Effect of involved Aspergillus species on galactomannan in bronchoalveolar lavage of patients with invasive aspergillosis.
CONCLUSION: Overall, the mean GM index in patients with A. fumigatus (3.1) was significantly higher than those of A. flavus (1.6; P-value=0.031) and the sensitivity of GM lower for A. flavus when compared to A. fumigatus. This finding has implications for diagnosis in hospitals and countries with a high proportion of A. flavus infections. PMID: 28693685 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 10, 2017 Category: Microbiology Authors: Taghizadeh-Armaki M, Hedayati MT, Moqarabzadeh V, Ansari S, Mahdavi Omran S, Zarrinfar H, Saber S, Verweij PE, Denning DW, Seyedmousavi S Tags: J Med Microbiol Source Type: research

Candidaemia in a tertiary care academic hospital in Italy. The impact of C. parapsilosis complex on the species distribution and antifungal susceptibility.
CONCLUSIONS: A progressively enhanced incidence of Candida BSIs, a relatively high impact of C. parapsilosis complex and changes in the susceptibility profiles of the isolated yeasts were evidenced during the observation period. PMID: 28686553 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 8, 2017 Category: Microbiology Authors: Ghezzi MC, Brunetti G, Visconti V, Giordano A, Raponi G Tags: J Med Microbiol Source Type: research

Molecular epidemiology of beta-lactamase producing nosocomial Gram-negative pathogens from North and South Indian hospitals.
CONCLUSION: Beta-lactamases showed tremendous variation in geographic distribution. Thus, their detection and characterization is important from a clinical-epidemiological, laboratory and infection control point of view. Knowledge of this epidemiology can predict the empiric antimicrobial treatment. PMID: 28671522 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 3, 2017 Category: Microbiology Authors: Khurana S, Mathur P, Kapil A, Valsan C, Behera B Tags: J Med Microbiol Source Type: research

Mycobacterium tuberculosis hypoxic response protein 1 (Hrp1) augments the pro-inflammatory response and enhances the survival of Mycobacterium smegmatis in murine macrophages.
CONCLUSION: M. tuberculosis protein Rv2626c plays a significant role in stimulating macrophages to provoke a pro-inflammatory response and in mycobacterial survival during infection. PMID: 28671529 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 3, 2017 Category: Microbiology Authors: Sun C, Yang G, Yuan J, Peng X, Zhang C, Zhai X, Luo T, Bao L Tags: J Med Microbiol Source Type: research

Comparative analysis of the genomes of clinical isolates of Mycobacterium avium subsp. hominissuis regarding virulence-related genes.
CONCLUSIONS: The sequences provided important information about M. avium heterogenicity and evolution as a pathogen. The limitation is the lack of understanding on possible overlapping functions of genes/proteins. PMID: 28671535 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 3, 2017 Category: Microbiology Authors: Jeffrey B, Rose SJ, Gilbert K, Lewis M, Bermudez LE Tags: J Med Microbiol Source Type: research

The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India.
CONCLUSION: Our results highlight the distribution of carbapenem- and colistin-resistance in Gram-negative bacteria isolated from the Tamil Nadu region in South India. PMID: 28671537 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 3, 2017 Category: Microbiology Authors: Manohar P, Shanthini T, Ayyanar R, Bozdogan B, Wilson A, Tamhankar AJ, Nachimuthu R, Lopes BS Tags: J Med Microbiol Source Type: research

Molecular mechanisms and epidemiology of resistance in Streptococcus pneumoniae in the Middle East region.
CONCLUSION: In order to better survey, control and prevent the emergence of multidrug- and extremely drug-resistant S. pneumoniae strains, antimicrobial stewardship, national surveillance and public awareness programmes should be developed urgently in Middle Eastern countries. PMID: 28650313 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 26, 2017 Category: Microbiology Authors: El Moujaber G, Osman M, Rafei R, Dabboussi F, Hamze M Tags: J Med Microbiol Source Type: research

Staphylococcus epidermidis lipoteichoic acid: exocellular release and ltaS gene expression in clinical and commensal isolates.
blero J, Cancino-Diaz ME, Cancino-Diaz JC Abstract PURPOSE: Staphylococcus epidermidis ATCC12228 lipoteichoic acid (LTA) inhibits TNFα production from keratinocytes that are activated with poly I:C. However, this effect has not been proven in clinical or commensal isolates. METHODOLOGY: The
Source: Journal of Medical Microbiology - June 22, 2017 Category: Microbiology Authors: García-Gómez E, Miranda-Ozuna JFT, Díaz-Cedillo F, Vázquez-Sánchez EA, Rodríguez-Martínez S, Jan-Roblero J, Cancino-Diaz ME, Cancino-Diaz JC Tags: J Med Microbiol Source Type: research

First description of methyltransferases in extensively drug-resistant Klebsiella pneumoniae isolates from Saudi Arabia.
CONCLUSIONS: To our knowledge, this is the first report describing XDRKPN encoding at least seven resistance determinants and harbouring methyltransferases in Saudi Arabia. PMID: 28639934 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 22, 2017 Category: Microbiology Authors: Abdalhamid B, Elhadi N, Albunayan S, Alsamman K, Aljindan R Tags: J Med Microbiol Source Type: research

Rapid detection of extended-spectrum- β-lactamase-producing Enterobacteriaceae in blood cultures using the ESBL NDP test in Cotonou, Benin.
CONCLUSION: The ESBL NDP test is rapid, relatively affordable and performed well in our setting. PMID: 28639543 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 21, 2017 Category: Microbiology Authors: Affolabi D, Sogbo F, Laleye G, Orekan J, Massou F, Kehinde A, Anagonou S Tags: J Med Microbiol Source Type: research

Infection and tissue repair of experimental cutaneous candidiasis in diabetic mice.
CONCLUSION: These findings highlight some important changes in diabetic animal responses to C. albicans infection that may be important to the pathophysiological processes underpinning cutaneous candidiasis in diabetic patients. PMID: 28604330 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2017 Category: Microbiology Authors: Pupim ACE, Campois TG, Araújo EJA, Svidizinski TIE, Felipe I Tags: J Med Microbiol Source Type: research

Evidence of persistence of Prevotella spp. in the cystic fibrosis lung.
CONCLUSION: The similarity in PFGE and RAPD banding patterns observed in sequential CF Prevotella isolates may be indicative of the persistence of this genus in the CF lung. Further work is required to determine the clinical significance of this finding, and to more accurately distinguish differences in pathogenicity between species. PMID: 28604331 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2017 Category: Microbiology Authors: Gilpin DF, Nixon KA, Bull M, McGrath SJ, Sherrard L, Rolain JM, Mahenthiralingam E, Elborn JS, Tunney MM Tags: J Med Microbiol Source Type: research

Performance of the Quantamatrix Multiplexed Assay Platform system for the differentiation and identification of Mycobacterium species.
CONCLUSION: The QMAP system yielded rapid, highly sensitive and specific results for the identification of MTBC and NTM and accurately discriminated between NTM species within 3 h. PMID: 28604333 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2017 Category: Microbiology Authors: Wang HY, Uh Y, Kim S, Lee H Tags: J Med Microbiol Source Type: research

Multi-locus variable-number tandem repeat analysis of Bordetella pertussis isolates circulating in Poland in the period 1959-2013.
CONCLUSIONS: Differences traced by MLVA in relation to the MLST and PFGE profiling confirmed that the B. pertussis strain types currently observed elsewhere in Europe, even if appearing in Poland, were not able to successfully disseminate within a human population in Poland that has been vaccinated with a whole-cell pertussis vaccine not used in other countries. PMID: 28598302 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Mosiej E, Krysztopa-Grzybowska K, Polak M, Prygiel M, Lutyńska A Tags: J Med Microbiol Source Type: research

Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion.
This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae. RESULTS: DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 µmol l-1, and minimum bactericidal concentration ranging between 69 and 277 µmol l-1. It significantly reduced bacterial biofilm survival - by 22-63 % (at MIC, 10×MIC or 100×MIC) as compared to the 25-42 % reduction by vancomycin (P
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Sardi JCO, Polaquini CR, Freires IA, Galvão LCC, Lazarini JG, Torrezan GS, Regasini LO, Rosalen PL Tags: J Med Microbiol Source Type: research

Comparison of the in vitro activity of ampicillin and moxifloxacin against Listeria monocytogenes at achievable concentrations in the central nervous system.
CONCLUSION: The results of this study demonstrated the faster bactericidal activity of moxifloxacin at achievable central nervous system concentrations against intra- and extracellular forms of L. monocytogenes in comparison with ampicillin. PMID: 28598305 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Pupo I, Lepe JA, Smani Y, Aznar J Tags: J Med Microbiol Source Type: research

Determinants of the regulation of Helicobacter pylori adhesins include repeat sequences in both promoter and coding regions as well as the two-component system ArsRS.
CONCLUSION: This work highlights the complex nature of adhesin regulation, its impact on H. pylori attachment and the pervasive role of ArsRS in adhesin expression. Such regulation may help facilitate the decades-long persistence of infection. PMID: 28598306 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Acio-Pizzarello CR, Acio AA, Choi EJ, Bond K, Kim J, Kenan AC, Chen J, Forsyth MH Tags: J Med Microbiol Source Type: research

Exposure to sub-inhibitory concentrations of gentamicin, ciprofloxacin and cefotaxime induces multidrug resistance and reactive oxygen species generation in meticillin-sensitive Staphylococcus aureus.
CONCLUSIONS: Our study demonstrates that sub-MIC levels of antimicrobials can lead to resistance and cross-resistance across several classes of antibiotics in wild strains of S. aureus, possibly by free radical production. The molecular mechanisms behind the acquisition of drug resistance at low antibiotic concentrations and the specific target genes of reactive oxygen speciesneed to be explored further. PMID: 28598307 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Bhattacharya G, Dey D, Das S, Banerjee A Tags: J Med Microbiol Source Type: research

In vitro activities of vancomycin and linezolid against biofilm-producing methicillin-resistant staphylococci species isolated from catheter-related bloodstream infections from an Egyptian tertiary hospital.
CONCLUSION: Vancomycin, linezolid and rifampicin could be used effectively against methicillin-resistant staphylococci isolated from CRBSIs. PMID: 28598310 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Hashem AA, Abd El Fadeal NM, Shehata AS Tags: J Med Microbiol Source Type: research

In vitro activity of sitafloxacin against Mycobacterium tuberculosis with gyrA/B mutations isolated in Japan.
CONCLUSION: SFX had the lowest cumulative MIC and a relatively low ECOFF value against Mtb, indicating that SFX was not only more effective against gyrA-mutant isolates, but also MDR isolates in Japan. PMID: 28598311 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Yi L, Aono A, Chikamatsu K, Igarashi Y, Yamada H, Takaki A, Mitarai S Tags: J Med Microbiol Source Type: research

Prevalence of faecal carriage of colistin-resistant Gram-negative rods in a university hospital in western France, 2016.
Abstract Plasmid-mediated and chromosomally-encoded colistin resistance is increasingly being reported worldwide. We aimed to determine the prevalence of faecal carriage of colistin-resistant Gram-negative rod isolates in a university hospital in western France. From February to May 2016, rectal swabs from 653 patients hospitalized in various clinical settings were recovered and subsequently screened for colistin resistance using the SuperPolymyxin medium. Antimicrobial susceptibilities were determined according to EUCAST guidelines. Genetic detection of plasmid-mediated colistin resistance was performed by PCR. T...
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Saly M, Jayol A, Poirel L, Megraud F, Nordmann P, Dubois V Tags: J Med Microbiol Source Type: research

How do the epidemiology of paediatric methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus bacteraemia differ?
Abstract PURPOSE: To examine whether the epidemiology of bacteraemia caused by methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) differed in children aged
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Abernethy J, Sharland M, Johnson AP, Hope R Tags: J Med Microbiol Source Type: research

Chlorhexidine whole-body washing of patients reduces methicillin-resistant Staphylococcus aureus and has a direct effect on the distribution of the ST5-MRSA-II (New York/Japan) clone.
CONCLUSION: Our findings suggest that the CXG bathing favored the reduction of healthcare-associated MRSA isolates and a temporary reduction of the predominant ST5-MRSA-II (New York/Japan) clone. PMID: 28590235 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: Velázquez-Meza ME, Mendoza-Olazarán S, Echániz-Aviles G, Camacho-Ortiz A, Martínez-Reséndez MF, Valero-Moreno V, Garza-González E Tags: J Med Microbiol Source Type: research

Evaluation of self-collected rectal swabs for the detection of bacteria responsible for sexually transmitted infections in a cohort of HIV-1-infected patients.
CONCLUSION: In conclusion, testing extra-genital sites is crucial for the diagnosis of STIs in men and women presenting or not concomitant urogenital infections in order to detect asymptomatic carriage with the aim of controlling and preventing transmission to their sexual partners. PMID: 28590237 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: Edouard S, Tamalet C, Tissot-Dupont H, Colson P, Ménard A, Ravaux I, Dhiver C, Tomei C, Stein A, Raoult D Tags: J Med Microbiol Source Type: research

What were the risk factors and trends in antimicrobial resistance for enteric fever in London 2005-2012?
Abstract PURPOSE: A study was undertaken to determine the risk factors and trends in antimicrobial resistance for enteric fever. METHODOLOGY: Demographic, antimicrobial susceptibility, typing and epidemiological data were examined for 2005-2012 in patients with enteric fever in London. Single and multivariable logistic regression was used to determine the risk factors associated with antibiotic resistance. RESULTS: 453 cases with Salmonella enterica subsp. enterica serovar Paratyphi A, 17 with S. Paratyphi B and 611 with S. enterica subsp. enterica serovar Typhi were examined. For travellers, 335 (88 %...
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: Dave J, Warburton F, Freedman J, de Pinna E, Grant K, Sefton A, Crawley-Boevey E, Godbole G, Holliman R, Balasegaram S Tags: J Med Microbiol Source Type: research

Detection of Actinomyces spp. in cervical exudates from women with cervical intraepithelial neoplasia or cervical cancer.
CONCLUSION: Patients with cervical cancer had a higher prevalence of the presence of Actinomyces compared to the CIN and control groups. This is the first study in which a deliberate search of this genus has been performed in women with cervical pathologies. The use of specific primers for each species facilitated their detection in comparison with traditional isolation methods. More information is necessary to understand the molecular mechanisms involved in the complex role that bacterial communities may play in the development of cancer (and vice versa). PMID: 28590243 [PubMed - as supplied by publisher] (Source: Jo...
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: García-García A, Coronel-Martínez J, Leon DC, Romero-Figueroa MDS, Caballero-Pantoja YE, Manzanares-Leal GL, Rodriguez-Morales M, Sandoval-Trujillo H, Ramírez-Durán N Tags: J Med Microbiol Source Type: research

Epidemiology of culture-confirmed infections of Streptococcus pneumoniae (2012-2015) and nasopharyngeal carriage in children and households in Taiwan (2014-2015).
CONCLUSION: The isolation of non-vaccine serotypes and unknown serotypes after the introduction of PCV13 in children highlights the importance of continued surveillance for emerging serotypes. PMID: 28590240 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 5, 2017 Category: Microbiology Authors: Janapatla RP, Su LH, Chen HH, Chang HJ, Tsai TC, Chen PY, Chen CL, Chiu CH Tags: J Med Microbiol Source Type: research