Prevalence and antimicrobial susceptibilities of Acinetobacter baumannii and non-baumannii Acinetobacters from Terengganu, Malaysia and their carriage of carbapenemase genes.
Abstract A total of 153 non-repeat Acinetobacter spp. clinical isolates obtained in 2015 from Hospital Sultanah Nur Zahirah (HSNZ) in Terengganu, Malaysia, were characterized. Identification of the isolates at species level was performed by ribosomal DNA restriction analysis (ARDRA) followed by sequencing of the rpoB gene. The majority of the isolates (n=128; 83.7 %) were A. baumannii while the rest were identified as A. nosocomialis (n=16), A. calcoaceticus (n=5), A. soli (n=2), A. berezeniae (n=1) and A. variabilis (n=1). Multidrug resistance (MDR) was most prevalent in A. baumannnii (66.4 %) whereas only on...
Source: Journal of Medical Microbiology - September 25, 2018 Category: Microbiology Authors: Mohd Rani F, A Rahman NI, Ismail S, Abdullah FH, Othman N, Alattraqchi AG, Cleary DW, Clarke SC, Yeo CC Tags: J Med Microbiol Source Type: research

Molecular analysis of Streptococcus pyogenes strains isolated from patients with recurrent pharyngitis after oral amoxicillin treatment.
CONCLUSION: When recurrent pharyngitis emerges more than 40 days after the last treatment, penicillin can be prescribed again because reinfection is suspected. However, when recurrent pharyngitis takes place within 40 days after completing the treatment, alternative drugs should be considered for retreatment because the pharyngitis is likely to be due to reactivation. PMID: 30247119 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 24, 2018 Category: Microbiology Authors: Saito M, Hirose M, Ichinose H, Villanueva SYAM, Yoshida SI Tags: J Med Microbiol Source Type: research

Candida africana in recurrent vulvovaginal candidiasis (RVVC) patients: frequency and phenotypic and genotypic characteristics.
CONCLUSION: In our sample, C. africana was only isolated from 7.8 % of the patients with RVVC. While size polymorphisms in HPW1 genes allowed us to differentiate C. africana from C. albicans, no evidence of sequence variation within the Iranian C. africana isolates was observed. PMID: 30248002 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 24, 2018 Category: Microbiology Authors: Naeimi B, Mirhendi H, Khamisipour G, Sadeghzadeh F, Ahmadi B Tags: J Med Microbiol Source Type: research

Gluconeogenic growth of Vibrio cholerae is important for competing with host gut microbiota.
CONCLUSION: Gluconeogenesis biosynthesis is important for the successful colonization of V. cholerae in a niche that is full of competing microbiota. PMID: 30248003 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 24, 2018 Category: Microbiology Authors: Wang J, Xing X, Yang X, Jung IJ, Hao G, Chen Y, Liu M, Wang H, Zhu J Tags: J Med Microbiol Source Type: research

Determination and genome-wide analysis of Epstein-Barr virus (EBV) sequences in EBV-associated gastric carcinoma from Guangdong, an endemic area of nasopharyngeal carcinoma.
In conclusion, this study determined the EBV genomes in EBVaGC from Guangdong and performed a detailed genome-wide analysis of GC-EBV, which would be helpful for further understanding of the relationship between EBV genomic variation and EBVaGC carcinogenesis. PMID: 30239329 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 21, 2018 Category: Microbiology Authors: Chen JN, Zhou L, Qiu XM, Yang RH, Liang J, Pan YH, Li HF, Peng GR, Shao CK Tags: J Med Microbiol Source Type: research

Review of vaccination in pregnancy to prevent pertussis in early infancy.
Abstract Maternal pertussis vaccination has been introduced in several countries to protect infants from birth until routine infant vaccination takes place. This review assesses existing evidence on the effectiveness and safety of immunization in pregnancy. The search was finalized in April 2017 and was based on searches using several databases. The selection criteria included any experimental or observational study reporting on the immunogenicity, effectiveness or safety of vaccination with a pertussis-containing vaccine in pregnant women and their infants. Following de-duplication and exclusions, we identified 8...
Source: Journal of Medical Microbiology - September 17, 2018 Category: Microbiology Authors: Campbell H, Gupta S, Dolan GP, Kapadia SJ, Kumar Singh A, Andrews N, Amirthalingam G Tags: J Med Microbiol Source Type: research

Comparison of the Cepheid Xpert Xpress Flu/RSV Assay to in-house Flu/RSV triplex real-time RT-PCR for rapid molecular detection of Influenza A, Influenza B and Respiratory Syncytial Virus in respiratory specimens.
This study compared the performance of the commercially available Xpert Xpress Flu/RSV assay to an in-house FluAB/RSV triplex real-time RT-PCR assay for the detection of influenza A/B viruses and respiratory syncitial virus (RSV) from both nasopharyngeal aspirate (NPA) and nasopharyngeal flocked swab (NPS). A total of 20 external quality assurance (EQA) samples and 172 clinical respiratory samples were tested prospectively using both the Xpert Xpress Flu/RSV assay and the in-house FluAB/RSV triplex assay. For the EQA samples, concordance rate was 100 % when tested with both assays. For clinical samples, there was 100 %...
Source: Journal of Medical Microbiology - September 12, 2018 Category: Microbiology Authors: Ho YII, Wong AH, Lai RWM Tags: J Med Microbiol Source Type: research

Evaluating the microbial pattern of periprosthetic joint infections of the hip and knee.
CONCLUSION: Patients suffering PJI present a certain risk profile with many comorbidities, e.g. high age and obesity. The observed microbiological pattern demonstrates the rise of high-resistance pathogens. PMID: 30207518 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 12, 2018 Category: Microbiology Authors: Rosteius T, Jansen O, Fehmer T, Baecker H, Citak M, Schildhauer TA, Geßmann J Tags: J Med Microbiol Source Type: research

Conventional culture method and qPCR using 16S rDNA for tissue bank: a comparison using a model of cardiac tissue contamination.
Abstract Real-time polymerase chain reaction (qPCR) using 16S rDNA is an alternative to conventional culture-based tests. The aim of this study was to compare the conventional culture method with qPCR using 16S rDNA in a model of cardiac tissue contamination. Samples of cardiac tissue for artificial contamination with Escherichia coli and control samples were submitted for DNA extraction, which was conducted by selective and alkaline lysis and purification steps. A standard curve for 16S rDNA was constructed to determine the efficiency and analytical sensitivity of the assay in concentrations from 106 to 102&thins...
Source: Journal of Medical Microbiology - September 12, 2018 Category: Microbiology Authors: Tasca Ribeiro VS, Tuon FF, Kraft L, Suss PH, Wollmann LC, Roderjan JG, Brito DA, Alexandrino F, Malgarin JS, Morello LG, da Costa FDA, Pillonetto M Tags: J Med Microbiol Source Type: research

Resistance pattern and distribution of carbapenemase and antiseptic resistance genes among multidrug-resistant Acinetobacter baumannii isolated from intensive care unit patients.
CONCLUSION: Our study revealed the high frequency of multidrug- and carbapenem-resistant isolates of A. baumannii in ICU patients, with a high prevalence of the genes blaOXA-23 and blaOXA-51. However, no apparent biocide resistance was seen in A. baumannii isolates. It appears that appropriate surveillance and control measures are essential to prevent the emergence and transmission of MDR A. baumannii in Iran. PMID: 30175954 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - September 3, 2018 Category: Microbiology Authors: Shirmohammadlou N, Zeighami H, Haghi F, Kashefieh M Tags: J Med Microbiol Source Type: research

Evaluation of rapid KPC carbapenemase detection method based on MALDI-TOF VITEK MS spectra analysis.
Abstract Clinical microbiology laboratories in hospital settings need to be able to identify patients who carry carbapenemase-producing bacterial strains quickly in order to contain their spread and initiate proper pharmacological therapy. The aim of this study was to confirm the correlation between KPC production and a characteristic mass spectrometry (MS) peak (11 109 Da±8) to validate the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS as a rapid screening tool. With this aim, 176 selected clinical samples that were KPC-producing and 260 control samples that were carbap...
Source: Journal of Medical Microbiology - August 31, 2018 Category: Microbiology Authors: Centonze AR, Bertoncelli A, Savio C, Orza P, Bedenić B, Mazzariol A Tags: J Med Microbiol Source Type: research

Correlation between serum cryptococcal antigen titre and meningitis in immunocompetent patients.
This study demonstrates a clear correlation between serum cryptococcal antigen titre and meningitis. While the serum titre is not definitive for meningitis, in resource-limited settings or cases where lumbar puncture may be contraindicated, this evidence may aid diagnosis and subsequent therapeutic decisions. PMID: 30136919 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 23, 2018 Category: Microbiology Authors: Gassiep I, Aye C, Armstrong M, Emeto TI, Heather CS, Norton RE Tags: J Med Microbiol Source Type: research

Role of BK polyomavirus (BKV) and Torque teno virus (TTV) in liver transplant recipients with renal impairment.
CONCLUSION: Our data indicate that the detection of BKV and TTV is not a risk factor for renal impairment after LT. A correlation of TTV and BKV viral load seems to be an indicator for the immune status of the host. PMID: 30136921 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 23, 2018 Category: Microbiology Authors: Herrmann A, Sandmann L, Adams O, Herrmann D, Dirks M, Widera M, Westhaus S, Kaiser R, di Cristanziano V, Manns MP, Korth J, Richter N, Anastasiou O, Timm J, von Hahn T, Ciesek S Tags: J Med Microbiol Source Type: research

Intranasal coinfection model allows for assessment of protein vaccines against nontypeable Haemophilus influenzae in mice.
CONCLUSION: The results from this study suggest that our NTHi AOM coinfection model is useful for assessing protein vaccines. PMID: 30136923 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 23, 2018 Category: Microbiology Authors: Michel LV, Kaur R, Zavorin M, Pryharski K, Khan MN, LaClair C, O'Neil M, Xu Q, Pichichero ME Tags: J Med Microbiol Source Type: research

Development and validation of a multiplex reverse transcript real-time PCR for E6/E7 mRNA detection of high-risk human papillomavirus.
CONCLUSION: The one-step multiplex RT-PCR for E6/E7 mRNA detection is a simple, fast, universally applicable, sensitive and highly specific method for hrHPV E6/E7 mRNA detection. It is reliable for cervical lesion screening and of potential value in future clinical applications. PMID: 30124401 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 20, 2018 Category: Microbiology Authors: Pan C, Zhou J, Lyu J, Ren X Tags: J Med Microbiol Source Type: research

Aerosolization of Mycobacterium avium and Mycobacterium abscessus from a household ultrasonic humidifier.
CONCLUSIONS: This report documents the potential for M. avium subspecies hominissuis and M. abscessus subspecies abscessus cells in drinking water to be aerosolized from one type of portable humidifier; an ultrasonic humidifier. Care should be taken in using an ultrasonic humidifier where an individual at risk for mycobacterial pulmonary disease could be exposed. PMID: 30113303 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2018 Category: Microbiology Authors: Hamilton LA, Falkinham JO Tags: J Med Microbiol Source Type: research

Characterization of pelvic and cervical microbiotas from patients with pelvic inflammatory disease.
CONCLUSION: The NGS-based method provides a more informative profile of pelvic microbiota than the culture method. The pelvic and cervical microbiota from the same patient were inconsistent with each other, and thus therapeutic decisions based on cervical microbiota may lead to antimicrobial misuse. PMID: 30113305 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2018 Category: Microbiology Authors: Wang Y, Zhang Y, Zhang Q, Chen H, Feng Y Tags: J Med Microbiol Source Type: research

Icelandic ovine Mycoplasma ovipneumoniae are variable bacteria that induce limited immune responses in vitro and in vivo.
CONCLUSIONS: The moderate immune responses seen in this study indicate that previous exposure to mycoplasmas is a challenge for vaccination of lambs against M. ovipneumoniae. However, an alternative vaccination strategy, e.g. utilizing a recombinant vaccine, may overcome this vaccination hurdle in endemic regions and we suggest a possible vaccine candidate. PMID: 30113306 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2018 Category: Microbiology Authors: Einarsdottir T, Gunnarsson E, Hjartardottir S Tags: J Med Microbiol Source Type: research

Excess body weight and age associated with the carriage of fluoroquinolone and third-generation cephalosporin resistance genes in commensal Escherichia coli from a cohort of urban Vietnamese children.
CONCLUSION: The carriage of genotypic and phenotypic antimicrobial resistance is highly prevalent among E. coli in healthy children in the community in Vietnam. Future investigations on the carriage of antimicrobial resistant organisms in LMICs should focus on the progression of carriage from birth and structure of the microbiome in obesity. PMID: 30113307 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 16, 2018 Category: Microbiology Authors: Thi Quynh Nhi L, Thanh Tuyen H, Duc Trung P, Do Hoang Nhu T, Duy PT, Hao CT, Thi Thanh Nhan N, Vi LL, Thi Diem Tuyet H, Thi Thuy Tien T, Van Vinh Chau N, Khanh Lam P, Thwaites G, Baker S Tags: J Med Microbiol Source Type: research

Microbial otitis media: recent advancements in treatment, current challenges and opportunities.
Abstract Otitis media (OM) is a common disease affecting humans, especially paediatric populations. OM refers to inflammation of the middle ear and can be broadly classified into two types, acute and chronic. Bacterial infection is one of the most common causes of OM. Despite the introduction of vaccines, the incidence of OM remains significantly high worldwide. In this mini-review article, we discuss the recent treatment modalities for OM, such as suspension gel, transcutaneous immunization, and intranasal and transtympanic drug delivery, including therapies that are currently undergoing clinical trials. We provi...
Source: Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Mittal R, Parrish JM, Soni M, Mittal J, Mathee K Tags: J Med Microbiol Source Type: research

Genomic reorganization by IS26 in a blaNDM-5-bearing FII plasmid of Klebsiella pneumoniae isolated from a patient in Japan.
Abstract An NDM-5-producing Klebsiella pneumoniae ST147 strain was isolated from a Japanese patient who had not travelled abroad in at least 5 years. Whole-genome sequencing revealed a genomic rearrangement in an FII plasmid harbouring blaNDM-5 due to the replicative transposition of IS26. A hypothetical structure was proposed for its ancestral plasmid, and comparative genomic analysis of the plasmid suggested the dissemination of structurally similar plasmids harbouring blaNDM-5 in Asian and Middle Eastern countries. PMID: 30074471 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Takeuchi D, Akeda Y, Yoshida H, Hagiya H, Yamamoto N, Nishi I, Yoshioka N, Sugawara Y, Sakamoto N, Shanmugakani RK, Deguchi M, Tomono K, Hamada S Tags: J Med Microbiol Source Type: research

Beyond proteostasis: Roles of type I chaperonins in bacterial pathogenesis.
This article provides a thorough reviews of our current understanding of the different mechanisms involving type I chaperonins during bacteria-host interactions, and suggests new areas to be explored and the potential of finding new targets for fighting bacterial infections. PMID: 30074472 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Ghazaei C, Line El Helou M Tags: J Med Microbiol Source Type: research

The endogenous antiseptic N-chlorotaurine irreversibly inactivates Chlamydia pneumoniae and Chlamydia trachomatis.
CONCLUSIONS: This is the first study demonstrating the antimicrobial activity of NCT against Chlamydiae. Clinical implications of these findings have to be investigated in further trials. PMID: 30074473 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Bellmann-Weiler R, Maass V, Arnitz R, Weiss G, Maass M, Nagl M Tags: J Med Microbiol Source Type: research

Genotyping based on thermal denaturation of amplification products identifies species of the Mycobacterium tuberculosis complex.
CONCLUSION: Sensitivity, specificity and positive and negative predictive values of the method are 100 % for the genus Mycobacterium, which resolves MB, MTB and MA genotypes. Species/genotype agreement is 97.7 % for the panel and 92.3 % for the MTC isolates. This method may be advantageously used to identify the most prevalent MTC species in humans. PMID: 30074475 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Domínguez-Zepahua M, Hernández-Arteaga S, López-Revilla R Tags: J Med Microbiol Source Type: research

Mycoplasma pneumoniae DNA detection and specific antibody class response in patients from two tertiary care hospitals in tropical Sri Lanka.
CONCLUSION: The serological evidence for M. pneumoniae infection in Sri Lanka comprised the following prevalences: 17.5 % (17/97), 7.1 % (13/183) and 1.4 % (2/138) in adults with pneumonia, bronchitis or pharyngitis, respectively. M. pneumoniae DNA was in 52.2 % (12/23) of seropositives and 15.4 % (4/26) of seronegatives. IgG was predominant in PCR positives and negatives. PMID: 30074476 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 3, 2018 Category: Microbiology Authors: Wijesooriya LI, Kok T, Perera J, Tilakarathne Y, Sunil-Chandra NP Tags: J Med Microbiol Source Type: research

Forensic investigation into the origin of Pseudomonas aeruginosa PA14 - old but not lost.
PMID: 30067168 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - August 1, 2018 Category: Microbiology Authors: Mathee K Tags: J Med Microbiol Source Type: research

Epidemiology of Pseudomonas aeruginosa in agricultural areas.
This study strongly disputes that notion until the modern molecular technology proves otherwise. This paper examines a spectrum of strains occurring in nature, their habitats, dissemination, their relationship to clinical strains, and the environmental conditions that favor their colonization of plants. The isolates were obtained from clinical specimens, plants, soil, and water. The identity of these strains was confirmed using pyocin typing and biochemical assays. The data reveal that agricultural soils, potted ornamental plants, hoses, fountains, and faucets frequently harbored P. aeruginosa. However, it was not commonly...
Source: Journal of Medical Microbiology - August 1, 2018 Category: Microbiology Authors: Schroth MN, Cho JJ, Green SK, Kominos SD, Microbiology Society Publishing Tags: J Med Microbiol Source Type: research

Characterization of the clonal subpopulation Fiocruz L1-130 of Leptospira interrogans in rats and dogs from Brazil.
CONCLUSION: Understanding the circulation of strain Fiocruz L1-130 is important for the implementation of appropriate control measures. Its circulation highlights the need to treat leptospirosis caused by L. interrogans serogroup Icterohaemorrhagiae as a zoonosis that acts in the human-animal-environment interface, as per the One Health approach. PMID: 30059000 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 30, 2018 Category: Microbiology Authors: Jaeger LH, Pestana CP, Carvalho-Costa FA, Medeiros MA, Lilenbaum W Tags: J Med Microbiol Source Type: research

Multicenter assessment of the rapid Unyvero Blood Culture molecular assay.
CONCLUSION: The Unyvero BCU Application provided fast, reliable results, while significantly improving turnaround time in blood culture diagnostics. PMID: 30051799 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Burrack-Lange SC, Personne Y, Huber M, Winkler E, Weile J, Knabbe C, Görig J, Rohde H Tags: J Med Microbiol Source Type: research

Multi-centric validation of an in-house-developed beacon-based PCR diagnostic assay kit for Chlamydia and Neisseria and portable fluorescence detector.
CONCLUSION: Our kit was found to be as sensitive and specific as commercially available kits. Its low cost and ease of use will make it suitable for the routine diagnosis of C. trachomatis and N. gonorrhoeae in the resource-limited settings of developing countries. PMID: 30051801 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Sachdev D, Wasnik K, Patel AL, Sonkar SC, Desai P, Mania-Pramanik J, Kerkar S, Sethi S, Sharma N, Mittal P, Ghope P, Khandhari A, Saluja D Tags: J Med Microbiol Source Type: research

Performance evaluation of five commercial assays in assessing seroprevalence of HEV antibodies among blood donors.
CONCLUSIONS: Due to discrepancies in the performance of various IgG and IgM assays, seroprevalence studies should be based on furher confirmatory testing for decisive conclusions to be reached. PMID: 30051802 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Al-Absi ES, Al-Sadeq DW, Younis MH, Yassine HM, Abdalla OM, Mesleh AG, Hadwan TA, Amimo JO, Thalib L, Nasrallah GK Tags: J Med Microbiol Source Type: research

Mycoplasma amphoriforme vs M. pneumoniae: similarities and differences between patient characteristics in a regional hospital in the Netherlands.
Abstract Mycoplasma amphoriforme is a species closely related to Mycoplasma pneumoniae, thus far with unknown clinical impact. The application of optimized diagnostics, better capable of differentiating between these two micro-organisms, identified a significant patient population positive for M. amphoriforme. The PCR designed by Ling et al. was used on respiratory samples that originally tested positive for M. pneumoniae (n=78), and identified 29 retrospectively as M. amphoriforme. The aim of this study is to describe and compare both groups. The group infected with M. amphoriforme was significantly older and mor...
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: van Schaik ML, Patberg KW, Wallinga JG, Wolfhagen MJHM, Bruijnesteijn van Coppenraet LES Tags: J Med Microbiol Source Type: research

Quantification of major constituents of biofilms in occluded pancreatic stents.
CONCLUSION: The majority of the pancreatic stents grew polymicro-organisms, and those from patients aged>50 years showed significant deposition of protein, which is a key element in biofilm formation. Understanding the constituents of the biofilms in pancreatic stents could be very useful in developing future strategies for the prevention of biofilm formation. PMID: 30051805 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Vaishnavi C, Gupta PK, Sharma M, Kochhar R Tags: J Med Microbiol Source Type: research

Effect of storage time and temperature on the detection of Pseudomonas aeruginosa, Acanthamoeba and Herpes Simplex Virus from corneal impression membranes.
Abstract The effect of storage time and temperature on the recovery of pathogen DNA from polytetrafluorethylene (PTFE) was investigated. PTFE impression membranes were inoculated with Pseudomonas aeruginosa, Herpes Simplex Virus-1 (HSV-1) or Acanthamoeba and stored at -70 °C, -20 °C, +4 °C or +35 °C. PCR was performed on days 0, 1, 2, 3, 7 and months 1, 3 and 10 post-inoculation. We found no reduction in the DNA recovery of any of the studied microorganisms for the first 3 days of storage up to +35 °C. For HSV-1 and P. aeruginosa, storage for 3 m...
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Somerville TF, Corless CE, Neal T, Kaye SB Tags: J Med Microbiol Source Type: research

High rates of Mycobacterium fortuitum isolation in respiratory samples from Iranian patients with suspected tuberculosis: is it clinically important?
CONCLUSION: These results demonstrated the high frequency of M. fortuitum in respiratory samples and that this bacterium causes transient infection or colonization in patients with underlying pulmonary conditions, such as cystic fibrosis and cigarette smoking-induced. Additionally, it appears that infection with M. fortuitum is particularly common and may be important in patients with HIV. PMID: 30052175 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Irandoost M, Zare Ghanbari M, Sakhaee F, Vaziri F, Rahimi Jamnani F, Siadat SD, Fateh A Tags: J Med Microbiol Source Type: research

Fukugiside, a biflavonoid from Garcinia travancorica inhibits biofilm formation of Streptococcus pyogenes and its associated virulence factors.
CONCLUSION: Fukugiside exhibits potent anti-biofilm and anti-virulence activity against different M serotypes of S. pyogenes. It is also non-toxic, which augurs well for its clinical application. PMID: 30052177 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Nandu TG, Subramenium GA, Shiburaj S, Viszwapriya D, Iyer PM, Balamurugan K, Rameshkumar KB, Karutha Pandian S Tags: J Med Microbiol Source Type: research

Time-kill kinetics of cadazolid and comparator antibacterial agents against different ribotypes of Clostridium difficile.
CONCLUSION: The data presented here demonstrate the potent in vitro bactericidal activity of cadazolid against different ribotypes of C. difficile, although on a limited set of strains. PMID: 30052178 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 27, 2018 Category: Microbiology Authors: Skinner K, Birchall S, Corbett D, Thommes P, Locher HH Tags: J Med Microbiol Source Type: research

Evaluation of the FilmArray Blood Culture Identification Panel compared to direct MALDI-TOF MS identification for rapid identification of pathogens.
Abstract To improve time to identification of pathogens and detection of resistance genes, we evaluated the BioFire FilmArray Blood Culture Identification Panel (BCID) as compared to: (1) direct MALDI-TOF MS (DM) and (2) standardized culture-based identification (ID) with antibiotic susceptibility testing (AST). BCID gave an accurate identification in 102/112 (91 %) of cases (102/103 for on-panel organisms). DM gave an accurate identification in 91/112 (81 %) of cases, with 13/91 (14 %) requiring repeat testing from the residual pellet. The mean time to an identification result was 2.4 and 2.9 h for B...
Source: Journal of Medical Microbiology - July 20, 2018 Category: Microbiology Authors: Payne M, Champagne S, Lowe C, Leung V, Hinch M, Romney MG Tags: J Med Microbiol Source Type: research

Pyrosequencing: a rapid and effective sequencing method to diagnose drug-resistant tuberculosis.
CONCLUSION: The PSQ assay is a rapid and effective method for detecting drug resistance mutations from Mtb clinical isolates in a short period of time. PMID: 30028665 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 20, 2018 Category: Microbiology Authors: Govindaswamy A, Sakthi D, Pai R, Jeyaseelan L, Michael JS Tags: J Med Microbiol Source Type: research

Development of a species-specific PCR-RFLP targeting rpoD gene fragment for discrimination of Aeromonas species.
CONCLUSION: This method provides an alternative method for laboratory identification, surveillance and epidemiological investigations of clinical and environmental specimens. PMID: 30024365 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 19, 2018 Category: Microbiology Authors: Puah SM, Khor WC, Kee BP, Tan JAMA, Puthucheary SD, Chua KH Tags: J Med Microbiol Source Type: research

Isolation and characterization of Leptospira interrogans from two patients with leptospirosis in Western Province, Sri Lanka.
This study reports the successful isolation of pathogenic Leptospira from two patients in Western Province, Sri Lanka. PMID: 30024367 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 19, 2018 Category: Microbiology Authors: Nisansala GGT, Muthusinghe D, Gunasekara TDCP, Weerasekera MM, Fernando SSN, Ranasinghe KNP, Marasinghe MGCP, Fernando PS, Koizumi N, Gamage CD Tags: J Med Microbiol Source Type: research

A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products.
CONCLUSION: The key factor in the timely conclusion of this investigation was intersectoral collaboration among epidemiologists, microbiologists, veterinarians, statisticians and health and food safety authorities at national, regional and local levels. PMID: 30024370 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 19, 2018 Category: Microbiology Authors: Anna D, Michela S, Giuliana B, Vicdalia Aniela A, Massimo A, Antonino B, Luca B, Patrizia C, Cesare C, Fabrizio C, Dario M, Marco DD, Violeta DM, Giovanni F, Alfonsina F, Stefano F, Antonietta G, Monica G, Caterina G, Fabrizia G, Maurilia M, Cristina M, D Tags: J Med Microbiol Source Type: research

Comparison of the clinical performance of OmniPlex-HPV and GeneFinder HPV for the detection and genotyping of human papillomaviruses in cervical specimens.
CONCLUSIONS: Our results suggest that OmniPlex-HPV and GeneFinder HPV are highly comparable for the detection and genotyping of HPV, but OmniPlex-HPV displays greater accuracy in cases of multiple HPV infection. PMID: 30024373 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Hong G, Park YG, Jin HW, Choi SK, Chi HY, Cho WJ, Lee JH, Han S, Hong SP Tags: J Med Microbiol Source Type: research

The potential of different molecular biology methods in tracking clones of Acinetobacter baumannii in an ICU setting.
CONCLUSION: M13 PCR can be used in routine environmental surveillance for the detection of persistent antibiotic resistant clones in an ICU setting because of its reliability and simplicity. Further studies based on greater sample size, conducted at the multi-centre level, can give us a better understanding of the reliability of the molecular methods that can be used for the detection of persistent clones in the hospital setting. PMID: 30016226 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Ravi NS, Anandan S, Vijayakumar S, Gopi R, Lopes BS, Veeraraghavan B Tags: J Med Microbiol Source Type: research

In vitro activity of β-lactams in combination with avibactam against multidrug-resistant Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Achromobacter xylosoxidans isolates from patients with cystic fibrosis.
In vitro activity of β-lactams in combination with avibactam against multidrug-resistant Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Achromobacter xylosoxidans isolates from patients with cystic fibrosis. J Med Microbiol. 2018 Jul 17;: Authors: Mathy V, Grohs P, Compain F Abstract The in vitro activity of anti-pseudomonal β-lactams in combination with avibactam was evaluated against 54 multidrug-resistant non-fermenting Gram-negative bacilli isolated from cystic fibrosis patients. Avibactam increased and/or restored the antibacterial activities of ceftazidime and aztreonam again...
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Mathy V, Grohs P, Compain F Tags: J Med Microbiol Source Type: research

Pasteurella multocida inactivated with ferric chloride and adjuvanted with bacterial DNA is a potent and efficacious vaccine in Balb/c mice.
CONCLUSION: These studies indicate that bDNA is effective as an immune adjuvant, and along with stimulatory bDNA represent promising new humoral and cellular immune enhancers for vaccination applications. In addition, this vaccine is able to provide long-term protection against infection. PMID: 30016232 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 17, 2018 Category: Microbiology Authors: Homayoon M, Tahamtan Y, Kargar M, Hosseini SMH, Akhavan Sepahy A Tags: J Med Microbiol Source Type: research

Comparative genome and evolution analysis of the locus of enterocyte effacement from enteropathogenic Escherichia coli Deng and its transcriptional response to ciprofloxacin.
CONCLUSIONS: The present findings provide an overview of the phylogenetic characteristics of EPEC Deng and its transcriptional response to ciprofloxacin, further suggesting that GrlA may play a clinically important role in EPEC responses to ciprofloxacin. PMID: 29989530 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 10, 2018 Category: Microbiology Authors: Chen Z, Cheng H, Pan W, Zheng J, Li D, Lin F, Yu Z, Deng Q Tags: J Med Microbiol Source Type: research

Accuracy of a commercial multiplex PCR for the diagnosis of bacterial vaginosis.
CONCLUSION: The ATRiDA test is a sensitive method for the detection of BV but, given the high occurrence of asymptomatic vaginal dysbiosis, a positive test outcome should be interpreted together with clinical symptoms. PMID: 29985123 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 9, 2018 Category: Microbiology Authors: van der Veer C, van Houdt R, van Dam A, de Vries H, Bruisten S Tags: J Med Microbiol Source Type: research

Bactibilia in women affected with diseases of the biliary tract and pancreas. A STROBE guidelines-adherent cross-sectional study in Southern Italy.
In this study, we aimed to assess the bile microbiological flora and its potential link with comorbidity in women. METHODOLOGY: We performed a microbiologic investigation on 53 female patients with biliopancreatic diseases who granted consent, and we analysed the data using a MATLAB platform. RESULTS: We found that the most frequent disease associated with bactibilia was pancreas head carcinoma (PHC) (P=0.0015), while the least frequent disease was gall bladder carcinoma (GBC) (P=0.0002). The most common microorganisms were Pseudomonas spp. (P
Source: Journal of Medical Microbiology - July 5, 2018 Category: Microbiology Authors: Serra N, Di Carlo P, Gulotta G, d' Arpa F, Giammanco A, Colomba C, Melfa G, Fasciana T, Sergi C Tags: J Med Microbiol Source Type: research

Hypervirulent Klebsiella pneumoniae, a 5-year study in a French ICU.
CONCLUSION: hvKp represent a potentially underestimated cause of fatal infections in the Western world. PMID: 29972348 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - July 4, 2018 Category: Microbiology Authors: Rafat C, Messika J, Barnaud G, Dufour N, Magdoud F, Billard-Pomarès T, Gaudry S, Dreyfuss D, Branger C, Decré D, Ricard JD Tags: J Med Microbiol Source Type: research