Whole genome sequencing of a clinical drug resistant < em > Candida albicans < /em > isolate reveals known and novel mutations in genes involved in resistance acquisition mechanisms
This study aims at determining point mutations in genes known to be involved in resistance acquisition and correlating resistance to virulence and ergosterol content in the azole resistant C. albicans isolate CA77 from Lebanon. This pilot study is the first of its kind to be implemented in Lebanon. We carried out whole genome sequencing of the azole resistant C. albicans isolate CA77 and examined 18 genes involved in antifungal resistance. To correlate genotype to phenotype, we evaluated the virulence potential of this isolate by injecting it into BALB/c mice and we quantified membrane ergosterol. Whole genome sequencing r...
Source: Journal of Medical Microbiology - April 28, 2021 Category: Microbiology Authors: Roy A Khalaf Nour Fattouh Matej Medvecky Jaroslav Hrabak Source Type: research

Rapid macrolide and amikacin resistance testing for < em > Mycobacterium abscessus < /em > in people with cystic fibrosis
Conclusion. Using the molecular methods described here, results for health care providers or researchers could be available days or weeks earlier than is currently possible via culture-based antibiotic susceptibility testing.PMID:33909552 | DOI:10.1099/jmm.0.001349 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 28, 2021 Category: Microbiology Authors: Amanda Bordin Sushil Pandey Christopher Coulter Melanie Syrmis Carolyn Pardo Hazel Hackett Scott C Bell Claire E Wainwright Graeme R Nimmo Amy V Jennison Julia E Clark David M Whiley Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Co-infection in critically ill patients with COVID-19: an observational cohort study from England
Conclusion. We found limited evidence for community-acquired bacterial co-infection in hospitalised adults with COVID-19, but a high rate of Gram-negative infection acquired during ICU stay.PMID:33861190 | DOI:10.1099/jmm.0.001350 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Vadsala Baskaran Hannah Lawrence Louise E Lansbury Karmel Webb Shahideh Safavi Nurul I Zainuddin Tausif Huq Charlotte Eggleston Jayne Ellis Clare Thakker Bethan Charles Sara Boyd Tom Williams Claire Phillips Ethan Redmore Sarah Platt Eve Hamilton Andrew B Source Type: research

Clinical characteristics and antibody response to SARS-CoV-2 spike 1 protein using VITROS Anti-SARS-CoV-2 antibody tests in COVID-19 patients in Japan
Conclusion. VITROS Anti-SARS-CoV-2 total and IgG assays will be useful as supporting diagnostic and surveillance tools and for evaluation of humoral immune response to COVID-19. Optimal prediction of disease prognosis is made from considering both clinical history and laboratory findings.PMID:33861191 | DOI:10.1099/jmm.0.001291 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 16, 2021 Category: Microbiology Authors: Mayu Nagura-Ikeda Kazuo Imai Katsumi Kubota Sakiko Noguchi Yutaro Kitagawa Masaru Matsuoka Sakiko Tabata Kazuyasu Miyoshi Toshimitsu Ito Kaku Tamura Takuya Maeda Source Type: research

Microbiome analysis of feline odontoclastic resorptive lesion (FORL) and feline oral health
J Med Microbiol. 2021 Apr;70(4). doi: 10.1099/jmm.0.001353.ABSTRACTIntroduction. Feline odontoclastic resorptive lesion (FORL) is one of the most common and painful oral diseases of the cat. It is characterised by tooth resorption due to destructive activity of odontoclasts. FORL can result in tooth loss. While the aetiology of FORL is not clearly understood, it is thought to be multifactorial and bacteria are likely to play a major role.Hypothesis. Dysbiosis of the normal feline oral microbiota leads to an alteration in commensal bacteria populations, which results in the development of FORL.Aim. The purpose of the curren...
Source: Journal of Medical Microbiology - April 15, 2021 Category: Microbiology Authors: Sheeba Thomas David F Lappin Christopher J Nile Julie Spears David Bennett Bernd W Brandt Marcello P Riggio Source Type: research

Development, deployment and in-field demonstration of mobile coronavirus SARS-CoV-2 Nucleic acid amplification test
Conclusion. Our mobile laboratory enables an adaptive response to increased test demand, and unlike many proprietary point-of-care PCR systems, rapid substitution with an alternative assay if gene targets change or reagent supply chains fail. We envisage operation of this RT-PCR assay as a standby capability to meet varying regional test demands under public health emergency operations guidance.PMID:33856292 | DOI:10.1099/jmm.0.001346 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 15, 2021 Category: Microbiology Authors: Teagan F Paton Ian Marr Zoe O'Keefe Timothy J J Inglis Source Type: research

Microbiome analysis of feline odontoclastic resorptive lesion (FORL) and feline oral health
J Med Microbiol. 2021 Apr;70(4). doi: 10.1099/jmm.0.001353.ABSTRACTIntroduction. Feline odontoclastic resorptive lesion (FORL) is one of the most common and painful oral diseases of the cat. It is characterised by tooth resorption due to destructive activity of odontoclasts. FORL can result in tooth loss. While the aetiology of FORL is not clearly understood, it is thought to be multifactorial and bacteria are likely to play a major role.Hypothesis. Dysbiosis of the normal feline oral microbiota leads to an alteration in commensal bacteria populations, which results in the development of FORL.Aim. The purpose of the curren...
Source: Journal of Medical Microbiology - April 15, 2021 Category: Microbiology Authors: Sheeba Thomas David F Lappin Christopher J Nile Julie Spears David Bennett Bernd W Brandt Marcello P Riggio Source Type: research

Development, deployment and in-field demonstration of mobile coronavirus SARS-CoV-2 Nucleic acid amplification test
Conclusion. Our mobile laboratory enables an adaptive response to increased test demand, and unlike many proprietary point-of-care PCR systems, rapid substitution with an alternative assay if gene targets change or reagent supply chains fail. We envisage operation of this RT-PCR assay as a standby capability to meet varying regional test demands under public health emergency operations guidance.PMID:33856292 | DOI:10.1099/jmm.0.001346 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 15, 2021 Category: Microbiology Authors: Teagan F Paton Ian Marr Zoe O'Keefe Timothy J J Inglis Source Type: research

Resistance to nitrofurantoin is an indicator of extensive drug-resistant (XDR) < em > Enterobacteriaceae < /em >
Conclusion. Nitrofurantoin resistance could be an indicator of the XDR phenotype among Enterobacteriaceae, harbouring multiple AMR and efflux pump genes. Tigecycline and colistin are the only antibiotics that could be used in the treatment of such XDR infections. A deeper understanding of the co-resistance mechanisms in XDR pathogens and prescription of AST-based appropriate combination therapy may help mitigate this problem.PMID:33830906 | DOI:10.1099/jmm.0.001347 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Balaram Khamari Prakash Kumar Bulagonda Eswarappa Pradeep Source Type: research

Quantification of < em > Vibrio cholerae < /em > cholix exotoxin by sandwich bead-ELISA
Conclusion. ChxA I to III was produced mostly extracellularly at various levels depending on strains and culture conditions. The bead-ELISA developed in this study is useful for the detection and quantification of ChxA in V. cholerae strains.PMID:33830907 | DOI:10.1099/jmm.0.001311 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Sharda Prasad Awasthi Nityananda Chowdhury Noritoshi Hatanaka Atsushi Hinenoya Thandavarayan Ramamurthy Masahiro Asakura Shinji Yamasaki Source Type: research

Environmental and clinical isolates of < em > Herbaspirillum < /em > induce pulmonary infection in mice and its secretome is cytotoxic to human lung cells
Conclusion. These in vivo and in vitro results provide insights into how some Herbaspirillum strains cause infection in humans, providing a basis for the characterization of pathogenesis studies on this emerging infectious agent.PMID:33830909 | DOI:10.1099/jmm.0.001343 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Josyel Olszewski Carolina Weigert Galv ão John J Lipuma Katia Sabrina Paludo Source Type: research

< em > Clostridioides difficile < /em > toxins enhanced the < em > in vitro < /em > production of CXC chemokine ligand 2 and tumor necrosis factor- α via Toll-like receptors in macrophages
Conclusion. Our results showed that C. difficile toxins, including CDT, enhanced macrophage-mediated CXCL2 and TNF-α production via TLR2 and TLR4, indicating that CDT affects host immune responses.PMID:33830910 | DOI:10.1099/jmm.0.001342 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hiroe Konishi McKee Chiaki Kajiwara Tetsuo Yamaguchi Yoshikazu Ishii Norikazu Shimizu Akira Ohara Kazuhiro Tateda Source Type: research

An antipathogenic compound that targets the OxyR peroxide sensor in < em > Pseudomonas aeruginosa < /em >
Conclusion. These results suggest that compound 1 could be an antipathogenic hit inhibiting the P. aeruginosa OxyR. More importantly, our study provides an insight into the computer-based discovery of new-paradigm selective antibacterials to treat Gram-negative bacterial infections presumably with few concerns of drug resistance.PMID:33830911 | DOI:10.1099/jmm.0.001341 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hyo-Young Oh Shivakumar S Jalde In-Young Chung Yeon-Ji Yoo Hye-Jeong Jang Hyun-Kyung Choi You-Hee Cho Source Type: research

Resistance to nitrofurantoin is an indicator of extensive drug-resistant (XDR) < em > Enterobacteriaceae < /em >
Conclusion. Nitrofurantoin resistance could be an indicator of the XDR phenotype among Enterobacteriaceae, harbouring multiple AMR and efflux pump genes. Tigecycline and colistin are the only antibiotics that could be used in the treatment of such XDR infections. A deeper understanding of the co-resistance mechanisms in XDR pathogens and prescription of AST-based appropriate combination therapy may help mitigate this problem.PMID:33830906 | DOI:10.1099/jmm.0.001347 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Balaram Khamari Prakash Kumar Bulagonda Eswarappa Pradeep Source Type: research

Quantification of < em > Vibrio cholerae < /em > cholix exotoxin by sandwich bead-ELISA
Conclusion. ChxA I to III was produced mostly extracellularly at various levels depending on strains and culture conditions. The bead-ELISA developed in this study is useful for the detection and quantification of ChxA in V. cholerae strains.PMID:33830907 | DOI:10.1099/jmm.0.001311 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Sharda Prasad Awasthi Nityananda Chowdhury Noritoshi Hatanaka Atsushi Hinenoya Thandavarayan Ramamurthy Masahiro Asakura Shinji Yamasaki Source Type: research

Environmental and clinical isolates of < em > Herbaspirillum < /em > induce pulmonary infection in mice and its secretome is cytotoxic to human lung cells
Conclusion. These in vivo and in vitro results provide insights into how some Herbaspirillum strains cause infection in humans, providing a basis for the characterization of pathogenesis studies on this emerging infectious agent.PMID:33830909 | DOI:10.1099/jmm.0.001343 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Josyel Olszewski Carolina Weigert Galv ão John J Lipuma Katia Sabrina Paludo Source Type: research

< em > Clostridioides difficile < /em > toxins enhanced the < em > in vitro < /em > production of CXC chemokine ligand 2 and tumor necrosis factor- α via Toll-like receptors in macrophages
Conclusion. Our results showed that C. difficile toxins, including CDT, enhanced macrophage-mediated CXCL2 and TNF-α production via TLR2 and TLR4, indicating that CDT affects host immune responses.PMID:33830910 | DOI:10.1099/jmm.0.001342 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hiroe Konishi McKee Chiaki Kajiwara Tetsuo Yamaguchi Yoshikazu Ishii Norikazu Shimizu Akira Ohara Kazuhiro Tateda Source Type: research

An antipathogenic compound that targets the OxyR peroxide sensor in < em > Pseudomonas aeruginosa < /em >
Conclusion. These results suggest that compound 1 could be an antipathogenic hit inhibiting the P. aeruginosa OxyR. More importantly, our study provides an insight into the computer-based discovery of new-paradigm selective antibacterials to treat Gram-negative bacterial infections presumably with few concerns of drug resistance.PMID:33830911 | DOI:10.1099/jmm.0.001341 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hyo-Young Oh Shivakumar S Jalde In-Young Chung Yeon-Ji Yoo Hye-Jeong Jang Hyun-Kyung Choi You-Hee Cho Source Type: research

Resistance to nitrofurantoin is an indicator of extensive drug-resistant (XDR) < em > Enterobacteriaceae < /em >
Conclusion. Nitrofurantoin resistance could be an indicator of the XDR phenotype among Enterobacteriaceae, harbouring multiple AMR and efflux pump genes. Tigecycline and colistin are the only antibiotics that could be used in the treatment of such XDR infections. A deeper understanding of the co-resistance mechanisms in XDR pathogens and prescription of AST-based appropriate combination therapy may help mitigate this problem.PMID:33830906 | DOI:10.1099/jmm.0.001347 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Balaram Khamari Prakash Kumar Bulagonda Eswarappa Pradeep Source Type: research

Quantification of < em > Vibrio cholerae < /em > cholix exotoxin by sandwich bead-ELISA
Conclusion. ChxA I to III was produced mostly extracellularly at various levels depending on strains and culture conditions. The bead-ELISA developed in this study is useful for the detection and quantification of ChxA in V. cholerae strains.PMID:33830907 | DOI:10.1099/jmm.0.001311 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Sharda Prasad Awasthi Nityananda Chowdhury Noritoshi Hatanaka Atsushi Hinenoya Thandavarayan Ramamurthy Masahiro Asakura Shinji Yamasaki Source Type: research

Environmental and clinical isolates of < em > Herbaspirillum < /em > induce pulmonary infection in mice and its secretome is cytotoxic to human lung cells
Conclusion. These in vivo and in vitro results provide insights into how some Herbaspirillum strains cause infection in humans, providing a basis for the characterization of pathogenesis studies on this emerging infectious agent.PMID:33830909 | DOI:10.1099/jmm.0.001343 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Josyel Olszewski Carolina Weigert Galv ão John J Lipuma Katia Sabrina Paludo Source Type: research

< em > Clostridioides difficile < /em > toxins enhanced the < em > in vitro < /em > production of CXC chemokine ligand 2 and tumor necrosis factor- α via Toll-like receptors in macrophages
Conclusion. Our results showed that C. difficile toxins, including CDT, enhanced macrophage-mediated CXCL2 and TNF-α production via TLR2 and TLR4, indicating that CDT affects host immune responses.PMID:33830910 | DOI:10.1099/jmm.0.001342 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hiroe Konishi McKee Chiaki Kajiwara Tetsuo Yamaguchi Yoshikazu Ishii Norikazu Shimizu Akira Ohara Kazuhiro Tateda Source Type: research

An antipathogenic compound that targets the OxyR peroxide sensor in < em > Pseudomonas aeruginosa < /em >
Conclusion. These results suggest that compound 1 could be an antipathogenic hit inhibiting the P. aeruginosa OxyR. More importantly, our study provides an insight into the computer-based discovery of new-paradigm selective antibacterials to treat Gram-negative bacterial infections presumably with few concerns of drug resistance.PMID:33830911 | DOI:10.1099/jmm.0.001341 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hyo-Young Oh Shivakumar S Jalde In-Young Chung Yeon-Ji Yoo Hye-Jeong Jang Hyun-Kyung Choi You-Hee Cho Source Type: research

Resistance to nitrofurantoin is an indicator of extensive drug-resistant (XDR) < em > Enterobacteriaceae < /em >
Conclusion. Nitrofurantoin resistance could be an indicator of the XDR phenotype among Enterobacteriaceae, harbouring multiple AMR and efflux pump genes. Tigecycline and colistin are the only antibiotics that could be used in the treatment of such XDR infections. A deeper understanding of the co-resistance mechanisms in XDR pathogens and prescription of AST-based appropriate combination therapy may help mitigate this problem.PMID:33830906 | DOI:10.1099/jmm.0.001347 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Balaram Khamari Prakash Kumar Bulagonda Eswarappa Pradeep Source Type: research

Quantification of < em > Vibrio cholerae < /em > cholix exotoxin by sandwich bead-ELISA
Conclusion. ChxA I to III was produced mostly extracellularly at various levels depending on strains and culture conditions. The bead-ELISA developed in this study is useful for the detection and quantification of ChxA in V. cholerae strains.PMID:33830907 | DOI:10.1099/jmm.0.001311 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Sharda Prasad Awasthi Nityananda Chowdhury Noritoshi Hatanaka Atsushi Hinenoya Thandavarayan Ramamurthy Masahiro Asakura Shinji Yamasaki Source Type: research

Environmental and clinical isolates of < em > Herbaspirillum < /em > induce pulmonary infection in mice and its secretome is cytotoxic to human lung cells
Conclusion. These in vivo and in vitro results provide insights into how some Herbaspirillum strains cause infection in humans, providing a basis for the characterization of pathogenesis studies on this emerging infectious agent.PMID:33830909 | DOI:10.1099/jmm.0.001343 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Josyel Olszewski Carolina Weigert Galv ão John J Lipuma Katia Sabrina Paludo Source Type: research

< em > Clostridioides difficile < /em > toxins enhanced the < em > in vitro < /em > production of CXC chemokine ligand 2 and tumor necrosis factor- α via Toll-like receptors in macrophages
Conclusion. Our results showed that C. difficile toxins, including CDT, enhanced macrophage-mediated CXCL2 and TNF-α production via TLR2 and TLR4, indicating that CDT affects host immune responses.PMID:33830910 | DOI:10.1099/jmm.0.001342 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hiroe Konishi McKee Chiaki Kajiwara Tetsuo Yamaguchi Yoshikazu Ishii Norikazu Shimizu Akira Ohara Kazuhiro Tateda Source Type: research

An antipathogenic compound that targets the OxyR peroxide sensor in < em > Pseudomonas aeruginosa < /em >
Conclusion. These results suggest that compound 1 could be an antipathogenic hit inhibiting the P. aeruginosa OxyR. More importantly, our study provides an insight into the computer-based discovery of new-paradigm selective antibacterials to treat Gram-negative bacterial infections presumably with few concerns of drug resistance.PMID:33830911 | DOI:10.1099/jmm.0.001341 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hyo-Young Oh Shivakumar S Jalde In-Young Chung Yeon-Ji Yoo Hye-Jeong Jang Hyun-Kyung Choi You-Hee Cho Source Type: research

Resistance to nitrofurantoin is an indicator of extensive drug-resistant (XDR) < em > Enterobacteriaceae < /em >
Conclusion. Nitrofurantoin resistance could be an indicator of the XDR phenotype among Enterobacteriaceae, harbouring multiple AMR and efflux pump genes. Tigecycline and colistin are the only antibiotics that could be used in the treatment of such XDR infections. A deeper understanding of the co-resistance mechanisms in XDR pathogens and prescription of AST-based appropriate combination therapy may help mitigate this problem.PMID:33830906 | DOI:10.1099/jmm.0.001347 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Balaram Khamari Prakash Kumar Bulagonda Eswarappa Pradeep Source Type: research

Quantification of < em > Vibrio cholerae < /em > cholix exotoxin by sandwich bead-ELISA
Conclusion. ChxA I to III was produced mostly extracellularly at various levels depending on strains and culture conditions. The bead-ELISA developed in this study is useful for the detection and quantification of ChxA in V. cholerae strains.PMID:33830907 | DOI:10.1099/jmm.0.001311 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Sharda Prasad Awasthi Nityananda Chowdhury Noritoshi Hatanaka Atsushi Hinenoya Thandavarayan Ramamurthy Masahiro Asakura Shinji Yamasaki Source Type: research

Environmental and clinical isolates of < em > Herbaspirillum < /em > induce pulmonary infection in mice and its secretome is cytotoxic to human lung cells
Conclusion. These in vivo and in vitro results provide insights into how some Herbaspirillum strains cause infection in humans, providing a basis for the characterization of pathogenesis studies on this emerging infectious agent.PMID:33830909 | DOI:10.1099/jmm.0.001343 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Josyel Olszewski Carolina Weigert Galv ão John J Lipuma Katia Sabrina Paludo Source Type: research

< em > Clostridioides difficile < /em > toxins enhanced the < em > in vitro < /em > production of CXC chemokine ligand 2 and tumor necrosis factor- α via Toll-like receptors in macrophages
Conclusion. Our results showed that C. difficile toxins, including CDT, enhanced macrophage-mediated CXCL2 and TNF-α production via TLR2 and TLR4, indicating that CDT affects host immune responses.PMID:33830910 | DOI:10.1099/jmm.0.001342 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 8, 2021 Category: Microbiology Authors: Hiroe Konishi McKee Chiaki Kajiwara Tetsuo Yamaguchi Yoshikazu Ishii Norikazu Shimizu Akira Ohara Kazuhiro Tateda Source Type: research