Encapsulated cyclosporine does not change the composition of the human microbiota when assessed ex vivo and in vivo.
Conclusion. SmPill minispheres with or without CyA at the clinically relevant doses tested here have negligible direct effects on the gut microbiota composition. Butyrate and acetate production increased, however, in the presence of the beads in an ex vivo model system as well as in vivo in healthy subjects. Importantly, this study also demonstrates the relevance and value of using ex vivo colon models to predict the in vivo impact of colon-targeted drugs directly on the gut microbiota. PMID: 31958048 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 20, 2020 Category: Microbiology Authors: O'Reilly C, O'Sullivan Ó, Cotter PD, O'Connor PM, Shanahan F, Cullen A, Rea MC, Hill C, Coulter I, Ross RP Tags: J Med Microbiol Source Type: research

Cefuroxime pharmacokinetics and pharmacodynamics for intravenous dosage regimens with 750 mg or 1500 mg doses in healthy young volunteers.
t-Møller N Abstract Introduction. Cefuroxime is an important antibiotic to treat several serious infections. Rapid elimination through the kidneys and the variation in MICs of various susceptible pathogens such as Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pneumoniae give rise to dosing issues, especially in otherwise healthy patients.Aim. To investigate the probability of target attainment (PTA) for obtaining the optimal dosage regimens for cefuroxime in healthy young people.Methodology. Two weeks apart 750 and 1500 mg cefuroxime were administered as an intraven...
Source: Journal of Medical Microbiology - January 20, 2020 Category: Microbiology Authors: Thønnings S, Jensen KS, Nielsen NB, Skjønnemand M, Hansen DS, Lange KHW, Frimodt-Møller N Tags: J Med Microbiol Source Type: research

The application of the CRISPR-Cas9 system in Pseudomonas syringae pv. actinidiae.
Conclusion . We have constructed shuttle plasmids carrying a CRISPR-Cas9 system that are maintained in both E. coli and P. syringae pv. actinidiae. We have used this gene editing system to eliminate features of the accessory genome (plasmids or ICEs) from Psa and to target a single chromosomal gene. PMID: 31935181 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 14, 2020 Category: Microbiology Authors: Ho J, Zhao M, Wojcik S, Taiaroa G, Butler M, Poulter R Tags: J Med Microbiol Source Type: research

Evaluation of chromogenic selective agar (CHROMagar STEC) for the direct detection of Shiga toxin-producing Escherichia coli from faecal specimens.
Abstract Shiga toxin-producing Escherichia coli (STEC) are zoonotic pathogens that cause symptoms of severe gastrointestinal disease, including haemolytic uraemic syndrome (HUS), in humans. Currently in England, STEC serotypes other than O157:H7 are not cultured at the local hospital laboratories. The aim of this study was to evaluate the utility of CHROMagar STEC for the direct detection of STEC from faecal specimens in a diagnostic setting, compared to the current reference laboratory method using PCR targeting the Shiga-toxin gene (stx) to test multiple colonies cultured on MacConkey agar. Of the 292 consecutiv...
Source: Journal of Medical Microbiology - January 14, 2020 Category: Microbiology Authors: Jenkins C, Perry NT, Godbole G, Gharbia S Tags: J Med Microbiol Source Type: research

There is no hiding if you Seq: recent breakthroughs in Pseudomonas aeruginosa research revealed by genomic and transcriptomic next-generation sequencing.
Abstract The advent of next-generation sequencing technology has revolutionized the field of prokaryotic genetics and genomics by allowing interrogation of entire genomes, transcriptomes and global transcription factor binding profiles. As more studies employing these techniques have been performed, the state of the art regarding prokaryotic gene regulation has developed from the level of individual genes to genetic regulatory networks and systems biology. When applied to bacterial pathogens, particularly valuable insights have been gained into the regulation of virulence-associated genes, their relative importanc...
Source: Journal of Medical Microbiology - January 14, 2020 Category: Microbiology Authors: Valli RXE, Lyng M, Kirkpatrick CL Tags: J Med Microbiol Source Type: research

An optimized algorithm with improved turnaround time for detection of carbapenemase-producing Enterobacterales using the NG Test CARBA 5 in a routine laboratory.
Conclusion . CARBA 5 performs well on colonies growing on CARBA Smart agar and significant improvements in TAT can be achieved without incurring additional costs or staff requirements. PMID: 31922949 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 10, 2020 Category: Microbiology Authors: Ratnayake L, Ang HZ, Ong CH, Chan DSG Tags: J Med Microbiol Source Type: research

In vitro activities of telithromycin against Staphylococcus aureus biofilms compared with azithromycin, clindamycin, vancomycin and daptomycin.
Conclusions. Telithromycin is more effective than azithromycin, clindamycin, vancomycin, or daptomycin against S. aureus biofilms. PMID: 31916929 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 9, 2020 Category: Microbiology Authors: Zheng JX, Tu HP, Sun X, Xu GJ, Chen JW, Deng QW, Yu ZJ, Qu D Tags: J Med Microbiol Source Type: research

In vivo acquisition and risk of inter-species spread of bla KPC-3-plasmid from Klebsiella pneumoniae to Serratia marcescens in the lower respiratory tract.
Abstract In recent years, Serratia marcescens has emerged as an important agent of hospital-acquired infections, such as pneumonia, urinary tract infection, septicaemia and meningitis, particularly in vulnerable patients. Compared to Klebsiella pneumoniae and Escherichia coli, S. marcescens is less commonly associated with bla KPC genes, yet few cases of plasmid transmission at the gastrointestinal level from K. pneumoniae carbapenemase (KPC)-producing Enterobacterales to S. marcescens have been described. Here we report a case of in vivo acquisition, during a 3-month period of hospitalization in the intensive car...
Source: Journal of Medical Microbiology - January 6, 2020 Category: Microbiology Authors: Bielli A, Piazza A, Cento V, Comandatore F, Lepera V, Gatti M, Brioschi P, Vismara C, Bandi C, Perno CF Tags: J Med Microbiol Source Type: research

A simple phenotypic test for detecting the contribution of outer membrane permeability to carbapenem resistance.
Conclusion. This paves the way for a diagnostic assay allowing the detection of this membrane-associated mechanism of resistance in Enterobacteriaceae. PMID: 31904320 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 6, 2020 Category: Microbiology Authors: Pinet E, Franceschi C, Collin V, Davin-Regli A, Zambardi G, Pagès JM Tags: J Med Microbiol Source Type: research

Genomic analyses of Pseudomonas syringae pv. actinidiae isolated in Korea suggest the transfer of the bacterial pathogen via kiwifruit pollen.
Conclusion. This analysis establishes that there have been multiple routes of transmission of the Psa3 pandemic strain into Korea. One route has involved the importation of pollen from New Zealand. A second route probably involves importation from Chile. PMID: 31859618 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 20, 2019 Category: Microbiology Authors: Kim GH, Lee YS, Jung JS, Koh YJ, Poulter RTM, Butler M Tags: J Med Microbiol Source Type: research

Pseudomonas aeruginosa mexT is an indicator of PAO1 strain integrity.
Abstract Laboratory research with Pseudomonas aeruginosa commonly involves the prototype strain PAO1. There is continued concern that PAO1 sublines maintained and propagated in the same laboratory or different laboratories exhibit genetic and phenotypic variability that may affect the reproducibility and validity of research. Whole-genome sequencing and other research identified the mexT locus as a mutational hotspot, but the explication of the diverse mutations present in the various sublines and consequences remained rather cursory. Here we present evidence that MexT sequence diversity is a predictor of PAO1 lin...
Source: Journal of Medical Microbiology - December 20, 2019 Category: Microbiology Authors: LoVullo ED, Schweizer HP Tags: J Med Microbiol Source Type: research

Biogenic selenium nanoparticles target chronic toxoplasmosis with minimal cytotoxicity in a mouse model.
This study aimed to evaluate the therapeutic effects of SeNPs against chronic toxoplasmosis in mice.Methodology. Infected mice with Toxoplasma gondii (Tehran strain) were orally treated with SeNPs at doses of 2.5, 5 and 10 mg kg-1 once a day for 14 days. On the fifthteenth day, the mean number of brain-tissue cysts and the mRNA levels of TNF-α, IL-12, IL-10, IFN-γ and inducible nitric oxide synthase (iNOS) in the mice of each group were recorded. Moreover, serum clinical chemistry factors in the treated mice were examined to determine the safety of SeNPs.Results. The mean number of tissue cysts was significantly (P
Source: Journal of Medical Microbiology - December 17, 2019 Category: Microbiology Authors: Keyhani A, Ziaali N, Shakibaie M, Kareshk AT, Shojaee S, Asadi-Shekaari M, Sepahvand M, Mahmoudvand H Tags: J Med Microbiol Source Type: research

Demographic, clinical and laboratory features of leptospirosis-malaria co-infections in Peninsular Malaysia.
Conclusion. The significant prevalence of LMCI found in this study with overlapping demographic, clinical and laboratory parameters makes diagnosis of co-infection challenging. It is essential to evaluate co-infection in endemic areas. Strengthened awareness of LMCI, comprehensive diagnostic services and further prospective studies are warranted. PMID: 31846413 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 17, 2019 Category: Microbiology Authors: Rao M, Atiqah N, Dasiman M, Amran F Tags: J Med Microbiol Source Type: research

Clinical presentations, genotypic diversity and phylogenetic analysis of Acanthamoeba species causing keratitis.
Conclusion. Rns genotyping is very effective in identifying the Acanthamoeba species and genotype in keratitis. Genotyping of Acanthamoeba spp. will help to advance our understanding of genotype-specific pathogenesis and geographical distribution. PMID: 31846414 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 17, 2019 Category: Microbiology Authors: Roshni Prithiviraj S, Rajapandian SGK, Gnanam H, Gunasekaran R, Mariappan P, Sankalp Singh S, Prajna L Tags: J Med Microbiol Source Type: research

A rapid, point-of-care antibiotic susceptibility test for urinary tract infections.
Conclusions. By reducing the typical timeframe for susceptibility testing from 2-3 days to 2 h, our POC phenotypic AST can provide critical information to clinicians prior to the administration of antibiotic therapy. PMID: 31846419 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 17, 2019 Category: Microbiology Authors: Toosky MN, Grunwald JT, Pala D, Shen B, Zhao W, D'Agostini C, Coghe F, Angioni G, Motolese G, Abram TJ, Nicolai E Tags: J Med Microbiol Source Type: research

The gut microbiome and neuropsychiatric disorders: implications for attention deficit hyperactivity disorder (ADHD).
Abstract Neuropsychiatric disorders (NPDs) such as depression, anxiety, bipolar disorder, autism spectrum disorder (ASD) and attention deficit hyperactivity disorder (ADHD) all relate to behavioural, cognitive and emotional disturbances that are ultimately rooted in disordered brain function. More specifically, these disorders are linked to various neuromodulators (i.e. serotonin and dopamine), as well as dysfunction in both cognitive and socio-affective brain networks. Increasing evidence suggests that the gut environment, and particularly the microbiome, plays a significant role in individual mental health. Alth...
Source: Journal of Medical Microbiology - December 10, 2019 Category: Microbiology Authors: Mathee K, Cickovski T, Deoraj A, Stollstorff M, Narasimhan G Tags: J Med Microbiol Source Type: research

The third quorum-sensing system of Pseudomonas aeruginosa: Pseudomonas quinolone signal and the enigmatic PqsE protein.
G, Cocotl-Yanez M Abstract Pseudomonas aeruginosa is an opportunistic pathogen that produces several virulence factors such as lectin A, pyocyanin, elastase and rhamnolipids. These compounds are controlled transcriptionally by three quorum-sensing circuits, two based on the synthesis and detection of N-acyl-homoserine-lactone termed the Las and Rhl system and a third system named the Pseudomonas quinolone signal (PQS) system, which is responsible for generating 2-alkyl-4(1 h)-quinolones (AQs). The transcriptional regulator called PqsR binds to the promoter of pqsABCDE in the presence of PQS or HHQ creating ...
Source: Journal of Medical Microbiology - December 3, 2019 Category: Microbiology Authors: García-Reyes S, Soberón-Chávez G, Cocotl-Yanez M Tags: J Med Microbiol Source Type: research

Reincubation of culture-negative urines for an additional 20 hours does not identify additional UTI cases.
Conclusion. Twenty hours of incubation did not have any negative effect on the detection of uropathogens. PMID: 31789587 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 2, 2019 Category: Microbiology Authors: Kocer K, Zimmermann S, Burckhardt I Tags: J Med Microbiol Source Type: research

Methicillin-resistant Staphylococcus epidermidis isolates with reduced vancomycin susceptibility from bloodstream infections in a neonatal intensive care unit.
Conclusion. The findings of this study demonstrated that blood invasive MR-S. epidermidis isolates recovered at NICU tend to show decreased vancomycin susceptibility making therapy of those fragile patients difficult. PMID: 31789588 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - December 2, 2019 Category: Microbiology Authors: Peixoto PB, Massinhani FH, Netto Dos Santos KR, Chamon RC, Silva RB, Lopes Correa FE, Barata Oliveira CDCH, Oliveira AG Tags: J Med Microbiol Source Type: research

Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment.
Abstract Introduction. Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome. Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.Aim. We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions. In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.Methodology. Six...
Source: Journal of Medical Microbiology - November 28, 2019 Category: Microbiology Authors: Huang R, Li F, Zhou Y, Zeng Z, He X, Fang L, Pan F, Chen Y, Lin J, Li J, Qiu D, Tian Y, Tan X, Song Y, Xu Y, Lai Y, Yi H, Gao Q, Fang X, Shi M, Zhou C, Huang J, He YT Tags: J Med Microbiol Source Type: research

Engineering of an Iranian Bordetella pertussis strain producing inactive pertussis toxin.
Conclusion. A B. pertussis of the predominant genomic and virulence lineage in Iran was successfully engineered to produce inactive PTX. This attenuated strain will be useful to further studies to develop both whole cell and acellular pertussis vaccines. PMID: 31778110 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 28, 2019 Category: Microbiology Authors: Nikbin VS, Keramati M, Noofeli M, Tayebzadeh F, Kahali B, Shahcheraghi F Tags: J Med Microbiol Source Type: research

Wide genetic heterogeneity and low antimicrobial resistance of enteroaggregative Escherichia coli isolates from several rural communities.
Abstract Enteroaggregative Escherichia coli (EAEC), a highly heterogeneous pathotype of E. coli classified as typical and atypical, are an emerging cause of acute and persistent diarrhea. We aimed to investigate whether population living in rural geographic areas, impacts in the heterogeneity, dissemination and antimicrobial susceptibility of EAEC strains. EAEC isolates (n=73) were analysed for the presence of 23 putative virulence factors, plasmid and antimicrobial resistance profiles, biofilm formation, pulsedfield gel electrophoresis (PFGE) and by multilocus sequence typing (MLST). The agg3A, agg4A, agn43, aap,...
Source: Journal of Medical Microbiology - November 26, 2019 Category: Microbiology Authors: Guerrieri CG, Monfardini MV, Silva EA, Bueno de Freitas L, Schuenck RP, Spano LC Tags: J Med Microbiol Source Type: research

Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia.
Conclusions. In a low-incidence setting, selective culture of respiratory samples on Ashdown was more sensitive and allowed quicker identification than routine media, at reasonable cost. Blood cultures are critical, confirming four cases missed by routine respiratory culture. Selective medium is useful in early pneumonia (pre-sepsis) and resource-limited settings where blood cultures are infrequently done. Real-time PCR is costly, but highly sensitive and useful for high-risk patients with diabetes, cancer or immunosuppressants, or requiring ventilation or intensive care. PMID: 31750812 [PubMed - as supplied by publis...
Source: Journal of Medical Microbiology - November 21, 2019 Category: Microbiology Authors: Subakir H, Chong YM, Chan YF, Hasan MS, Jamaluddin MFH, Pang YK, Ponnampalavanar S, Syed Omar SF, Sam IC Tags: J Med Microbiol Source Type: research

Pseudomonas aeruginosa and microbial keratitis.
Abstract Pseudomonas aeruginosa, a versatile Gram-negative pathogen that can cause a wide range of infections, is the most common causative agent in cases of bacterial keratitis associated with contact-lens use. Corneal infections with P. aeruginosa often have poor clinical outcomes and can result in long and costly treatments. During the infection process, the pathogen exploits its large genome, encoding complex regulatory networks and a wide range of virulence factors, including motility and the secretion of various proteases and toxins. Although antibiotic resistance levels in the UK are low, higher levels have...
Source: Journal of Medical Microbiology - November 21, 2019 Category: Microbiology Authors: Hilliam Y, Kaye S, Winstanley C Tags: J Med Microbiol Source Type: research

Molecular epidemiology and antifungal susceptibility profiles of clinical Cryptococcus neoformans/Cryptococcus gattii species complex.
Conclusion. Our findings may significantly contribute to the development of management strategies for patients at a higher risk of cryptococcosis, particularly HIV-positive individuals. PMID: 31750814 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 21, 2019 Category: Microbiology Authors: Bandalizadeh Z, Shokohi T, Badali H, Abastabar M, Babamahmoudi F, Davoodi L, Mardani M, Javanian M, Cheraghmakani H, Sepidgar AA, Badiee P, Khodavaisy S, Afshari SAK, Ahmadikia K, Seyedmousavi S Tags: J Med Microbiol Source Type: research

Editorial Conference Report 6th Midlands Molecular Microbiology Meeting (M4) 2019, 9/10 September, Nottingham University Jubilee Conference Centre, UK.
PMID: 31750815 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 21, 2019 Category: Microbiology Authors: Hardie KR Tags: J Med Microbiol Source Type: research

A positive BAL galactomannan in non-haemato-oncology patients risks harmful overtreatment.
Conclusion. We show that vigilance is required when interpreting galactomannan results in non-haematology patients to avoid potentially harmful overtreatment. PMID: 31746725 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 20, 2019 Category: Microbiology Authors: Martinelli AW, Patil P, Wong VK, Enoch DA, Sander CR Tags: J Med Microbiol Source Type: research

First detected OXA-50 carbapenem-resistant clinical isolates Pseudomonas aeruginosa from Bulgaria and interplay between the expression of main efflux pumps, OprD and intrinsic AmpC.
Conclusion. The increased expression or overexpression of MexXY-OprM efflux pumps is the leading cause of carbapenem resistance in our isolates Pseudomonas, detected in 94 % of the bacteria investigated. PMID: 31746726 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 20, 2019 Category: Microbiology Authors: Petrova A, Feodorova Y, Miteva-Katrandzhieva T, Petrov M, Murdjeva M Tags: J Med Microbiol Source Type: research

The combination of Corynebacterium pseudotuberculosis recombinant proteins rPLD, rCP01850 and rCP09720 for improved detection of caseous lymphadenitis in sheep by ELISA.
Abstract Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a chronic disease of sheep and goats. Current methods for CLA diagnosis cannot identify all infected animals; therefore, the development of an improved diagnosis is essential. We evaluated recombinant phospholipase D (rPLD) protein individually or combined with rCP01850 or rCP09720 proteins for the detection of CLA in sheep. A total of 40 positive and 25 negative sera samples were analysed by ELISA using the recombinant proteins. ELISA using rPLD (E1), rPLD+rCP01850 (E2) and rPLD+rCP09720 (E3) showed 90, 92.5 and 97....
Source: Journal of Medical Microbiology - November 14, 2019 Category: Microbiology Authors: Silva MTO, Bezerra FSB, de Pinho RB, de Santana Ferreira C, Vivas WL, Portela RWD, Azevedo VAC, Borsuk S Tags: J Med Microbiol Source Type: research

Plasmid-related β-lactamase genes in Pseudomonas aeruginosa isolates: a molecular study in burn patients.
Conclusion. All P. aeruginosa strains producing ESBLs had plasmids containing related genes. The data indicated a high prevalence of ESBL among P. aeruginosa isolates in the southwest of the Iran burn center and their enzyme types were diverse. PMID: 31718745 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 13, 2019 Category: Microbiology Authors: Rajaee Behbahani M, Keshavarzi A, Pirbonyeh N, Javanmardi F, Khoob F, Emami A Tags: J Med Microbiol Source Type: research

The effects of human lactoferrin in experimentally induced systemic candidiasis.
Conclusion. We concluded that hLF-treated Ltf -/- mice can reduce the severity of C. albicans-induced systemic infection. PMID: 31702539 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 8, 2019 Category: Microbiology Authors: Velliyagounder K, Rozario SD, Fine DH Tags: J Med Microbiol Source Type: research

Levonadifloxacin (WCK 771) exerts potent intracellular activity against Staphylococcus aureus in THP-1 monocytes at clinically relevant concentrations.
Conclusions. Within a clinically achievable concentration range, levonadifloxacin achieved a 90-99 % intracellular reduction of MSSA and MRSA strains phagocytized in THP-1 monocytes. Therefore, levonadifloxacin has the potential to be a therapeutic option for the management of intracellular methicillin- and quinolone-resistant staphylococcal infections. PMID: 31689174 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 5, 2019 Category: Microbiology Authors: Dubois J, Dubois M Tags: J Med Microbiol Source Type: research

Corrigendum: Multidrug- and colistin-resistant Salmonella enterica 4,[5],12:i:- sequence type 34 carrying the mcr-3.1 gene on the IncHI2 plasmid recovered from a human.
PMID: 31685092 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 1, 2019 Category: Microbiology Authors: Monte DF, Nelson V, Cerdeira L, Keelara S, Greene S, Griffin D, Rath S, Hall R, Page N, Lawson T, Springer D, Fedorka-Cray PJ, Thakur S Tags: J Med Microbiol Source Type: research

Corrigendum: Molecular characterization and antifungal susceptibility testing of Candida nivariensis from blood samples - an Iranian multicentre study and a review of the literature.
PMID: 31685093 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 1, 2019 Category: Microbiology Authors: Arastehfar A, Daneshnia F, Salehi MR, Zarrinfar H, Khodavaisy S, Haas PJ, Roudbary M, Najafzadeh MJ, Zomorodian K, Charsizadeh A, Brouwer C, Pan W, Hagen F, Boekhout T Tags: J Med Microbiol Source Type: research

Staphylococcus aureus products subvert the Burkholderia cenocepacia-induced inflammatory response in airway epithelial cells.
Conclusion. This study demonstrates for the first time the host response in a S. aureus/B. cenocepacia co-infection model and provides insight into a staphylococcal immune evasion mechanism, as well as a therapeutic intervention for excessive inflammation. PMID: 31674896 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 1, 2019 Category: Microbiology Authors: Ji Y, Bolhuis A, Watson ML Tags: J Med Microbiol Source Type: research

Carriage of Neisseria meningitidis and other Neisseria species among children and young adults in Paraguay.
Conclusion. Prevalence of N. meningitidis carriage was low compared to that reported from other settings, however, the overall carriage of Neisseria spp. (including N. meningitidis) was comparable to meningococcal carriage prevalence reported in the literature. This study is the first of its kind conducted in Paraguay, and one of the few known in the Southern Cone of Latin America. PMID: 31674897 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - November 1, 2019 Category: Microbiology Authors: Chamorro G, Ibarz-Pavon AB, Kawabata A, León ME, Orrego V, Nagai M, Gabastou JM Tags: J Med Microbiol Source Type: research

Clinical significance of Pseudomonas aeruginosa 2-alkyl-4-quinolone quorum-sensing signal molecules for long-term outcomes in adults with cystic fibrosis.
Conclusion. Further investigation to confirm the hypothesis that C9-PQS may be associated with increased antibiotic usage for pulmonary exacerbations is warranted as AQ-dependent signalling is a potential future target for anti-virulence therapies. PMID: 31671047 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 31, 2019 Category: Microbiology Authors: Webb K, Fogarty A, Barrett DA, Nash EF, Whitehouse JL, Smyth AR, Stewart I, Knox A, Williams P, Halliday N, Cámara M, Barr HL Tags: J Med Microbiol Source Type: research

Microcolonies: a novel morphological form of pathogenic Mycoplasma spp.
Conclusion. MCs play an unknown role in infection pathology and display prominent antibiotic resistance, making them a challenge for the future studies on Mollicutes. PMID: 31671056 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 31, 2019 Category: Microbiology Authors: Rakovskaya IV, Ermolaeva SA, Levina GA, Barkhatova OI, Mukhachev AY, Andreevskaya SG, Zhukhovitsky VG, Gorina LG, Miller GG, Sysolyatina EV Tags: J Med Microbiol Source Type: research

Same-day antimicrobial susceptibility test using acoustic-enhanced flow cytometry visualized with supervised machine learning.
Conclusions. The combination of machine learning with the FAST method generated same-day AST results and has the potential to aid early antimicrobial treatment decisions, stewardship and detection of resistance. PMID: 31665100 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 30, 2019 Category: Microbiology Authors: Inglis TJJ, Paton TF, Kopczyk MK, Mulroney KT, Carson CF Tags: J Med Microbiol Source Type: research

First Identification and genomic characterization of multidrug-resistant carbapenemase-producing Enterobacteriaceae clinical isolates in Malawi, Africa.
Conclusion. This report strongly suggests a probable existence of highly resistant various types of CPE organisms in Malawi including KPC-2-producing K. pneumoniae ST340/CC258, a known high-risk epidemic lineage. PMID: 31661049 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 29, 2019 Category: Microbiology Authors: Kumwenda GP, Sugawara Y, Abe R, Akeda Y, Kasambara W, Chizani K, Takeuchi D, Sakamoto N, Tomono K, Hamada S Tags: J Med Microbiol Source Type: research

Modified PFGE protocol for improving typeability of DNA degradation susceptible nosocomial Klebsiella pneumoniae.
Conclusion. We have successfully devised a PFGE protocol that enhanced the typeability of nosocomial K. pneumoniae. PMID: 31661052 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 29, 2019 Category: Microbiology Authors: Zakaria AM, Hassuna NA Tags: J Med Microbiol Source Type: research

Emerging multidrug resistance isolates of hospital-acquired bacterial meningitis in a tertiary care centre in North India.
Conclusion. In hospital-acquired bacterial meningitis, multidrug resistance Gram negative bacteria are a huge challenge for the treatment of patients. Hence, antimicrobial stewardship should be followed to counteract with the emerging multidrug resistance isolates. PMID: 31647400 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 24, 2019 Category: Microbiology Authors: Raza MS, Das BK, Goyal V, Lodha R, Chaudhry R, Sood S, Gautam H, Sreenivas V, Nair D, Mohapatra S, Kapil A Tags: J Med Microbiol Source Type: research

Risk factors for the first episode of Acinetobacter baumannii resistant to colistin infection and outcome in critically ill patients.
Abstract Objective. To identify risk factors for the first episode of Acinetobacter baumannii resistant to colistin (ABCR) infection in critically ill patients.Design. Prospective observational study.Setting. Twelve-bed general intensive care unit (ICU) at a University Hospital.Methods. ICU patients who required mechanical ventilation for>48 h during a 36 month period. Clinical and microbiological data were studied; characteristics of patients infected with ABCR were compared with those of critically ill patients who presented infection due to A. baumannii sensitive to colistin (ABCS).Results. Twe...
Source: Journal of Medical Microbiology - October 24, 2019 Category: Microbiology Authors: Mantzarlis K, Makris D, Zakynthinos E Tags: J Med Microbiol Source Type: research

An in vitro experimental study of the effect of fosfomycin in combination with amikacin, ciprofloxacin or meropenem on biofilm formation by multidrug-resistant urinary isolates of Escherichia coli.
Conclusion. The combination of fosfomycin with amikacin and fosfomycin with meropenem yielded a high percentage of synergy alongside an increased capacity to reduce biofilm formation when compared to combination with ciprofloxacin against multidrug-resistant E. coli. Fosfomycin in combination with other classes of antimicrobial agents has potential beneficial effects. PMID: 31647407 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 24, 2019 Category: Microbiology Authors: Sugathan S, Mandal J Tags: J Med Microbiol Source Type: research

Lactobacillus fermentum strains of dairy-product origin adhere to mucin and survive digestive juices.
Conclusion. These preliminary findings suggest a functionality in the two strains of L. fermentum with high colonization potential on GIT mucosal membranes and possible health-promoting effects. This prima facie evidence suggests the need for further studies to test these probiotic candidates as live biotherapeutic agents in vivo. PMID: 31613203 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Aziz K, Haseeb Zaidi A, Fatima HN, Tariq M Tags: J Med Microbiol Source Type: research

A novel multilocus variable-number tandem repeat analysis for Bordetella parapertussis.
Conclusion. Our novel MLVA method has high discriminatory power for genotyping human B. parapertussis. Regarding this organism, this genotyping system is a promising tool for epidemiological surveillance and investigating outbreaks. PMID: 31613204 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Kamachi K, Otsuka N, Fumimoto R, Ozawa K, Yao SM, Chiang CS, Luu LDW, Lan R, Shibayama K, Watanabe M Tags: J Med Microbiol Source Type: research

Rapid and accurate diagnosis of Chlamydia trachomatis in the urogenital tract by a dual-gene multiplex qPCR method.
Conclusion. In this study, a multiplex qPCR assay with high sensitivity and specificity for rapid (≤2.0 h) and accurate diagnosis of C. trachomatis was developed. The qPCR assay has the potential to be used as a routine diagnostic method in clinical microbiology laboratories. PMID: 31613208 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 15, 2019 Category: Microbiology Authors: Ma C, Du J, He W, Chen R, Li Y, Dou Y, Yuan X, Zhao L, Gong H, Liu P, Liu H Tags: J Med Microbiol Source Type: research

Myeloid C-type lectin receptors that recognize fungal mannans interact with Pneumocystis organisms and major surface glycoprotein.
In this study, we utilized a library of CLRs that recognize fungal mannans. We used this library to screen against Pneumocystis carinii (Pc) homogenates or purified Pc major surface glycoprotein (Msg) present on Pneumocystis. The results demonstrated that all of the mammalian CLR hFc-fusions tested displayed significant interaction/binding with Pc organisms, and furthermore to isolated Msg. Highest Pc organism and Msg binding activities were with CLR members Mincle, Dectin-2, DC-SIGN and MCL. An immunofluorescence assay with the respective CLR hFc-fusions against whole Pc life forms corroborated these findings. Although so...
Source: Journal of Medical Microbiology - October 14, 2019 Category: Microbiology Authors: Kottom TJ, Hebrink DM, Monteiro JT, Lepenies B, Carmona EM, Wuethrich M, Santo Dias LD, Limper AH Tags: J Med Microbiol Source Type: research

'Medical Mycology' - a new section in the Journal of Medical Microbiology.
PMID: 31609199 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 14, 2019 Category: Microbiology Authors: Chakrabarti A Tags: J Med Microbiol Source Type: research

Laboratory tools for tuberculosis control in a setting with a high burden of HIV/AIDS.
Conclusion. Putative nosocomial transmission of M. tuberculosis was detected, showing that genotyping is a powerful approach for understanding the dynamics of M. tuberculosis transmission, especially in a high-burden TB and HIV landscape. PMID: 31596198 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - October 9, 2019 Category: Microbiology Authors: Reis AJ, Diniz JLCG, Silva ABS, Silveira J, Basso R, Vieira R, von Groll A, Ramis IB, da Silva PEA Tags: J Med Microbiol Source Type: research