Infection and tissue repair of experimental cutaneous candidiasis in diabetic mice.
CONCLUSION: These findings highlight some important changes in diabetic animal responses to C. albicans infection that may be important to the pathophysiological processes underpinning cutaneous candidiasis in diabetic patients. PMID: 28604330 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2017 Category: Microbiology Authors: Pupim ACE, Campois TG, Araújo EJA, Svidizinski TIE, Felipe I Tags: J Med Microbiol Source Type: research

Evidence of persistence of Prevotella spp. in the cystic fibrosis lung.
CONCLUSION: The similarity in PFGE and RAPD banding patterns observed in sequential CF Prevotella isolates may be indicative of the persistence of this genus in the CF lung. Further work is required to determine the clinical significance of this finding, and to more accurately distinguish differences in pathogenicity between species. PMID: 28604331 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2017 Category: Microbiology Authors: Gilpin DF, Nixon KA, Bull M, McGrath SJ, Sherrard L, Rolain JM, Mahenthiralingam E, Elborn JS, Tunney MM Tags: J Med Microbiol Source Type: research

Performance of the Quantamatrix Multiplexed Assay Platform system for the differentiation and identification of Mycobacterium species.
CONCLUSION: The QMAP system yielded rapid, highly sensitive and specific results for the identification of MTBC and NTM and accurately discriminated between NTM species within 3 h. PMID: 28604333 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 13, 2017 Category: Microbiology Authors: Wang HY, Uh Y, Kim S, Lee H Tags: J Med Microbiol Source Type: research

Multi-locus variable-number tandem repeat analysis of Bordetella pertussis isolates circulating in Poland in the period 1959-2013.
CONCLUSIONS: Differences traced by MLVA in relation to the MLST and PFGE profiling confirmed that the B. pertussis strain types currently observed elsewhere in Europe, even if appearing in Poland, were not able to successfully disseminate within a human population in Poland that has been vaccinated with a whole-cell pertussis vaccine not used in other countries. PMID: 28598302 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Mosiej E, Krysztopa-Grzybowska K, Polak M, Prygiel M, Lutyńska A Tags: J Med Microbiol Source Type: research

Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion.
This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae. RESULTS: DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 µmol l-1, and minimum bactericidal concentration ranging between 69 and 277 µmol l-1. It significantly reduced bacterial biofilm survival - by 22-63 % (at MIC, 10×MIC or 100×MIC) as compared to the 25-42 % reduction by vancomycin (P
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Sardi JCO, Polaquini CR, Freires IA, Galvão LCC, Lazarini JG, Torrezan GS, Regasini LO, Rosalen PL Tags: J Med Microbiol Source Type: research

Comparison of the in vitro activity of ampicillin and moxifloxacin against Listeria monocytogenes at achievable concentrations in the central nervous system.
CONCLUSION: The results of this study demonstrated the faster bactericidal activity of moxifloxacin at achievable central nervous system concentrations against intra- and extracellular forms of L. monocytogenes in comparison with ampicillin. PMID: 28598305 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Pupo I, Lepe JA, Smani Y, Aznar J Tags: J Med Microbiol Source Type: research

Determinants of the regulation of Helicobacter pylori adhesins include repeat sequences in both promoter and coding regions as well as the two-component system ArsRS.
CONCLUSION: This work highlights the complex nature of adhesin regulation, its impact on H. pylori attachment and the pervasive role of ArsRS in adhesin expression. Such regulation may help facilitate the decades-long persistence of infection. PMID: 28598306 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Acio-Pizzarello CR, Acio AA, Choi EJ, Bond K, Kim J, Kenan AC, Chen J, Forsyth MH Tags: J Med Microbiol Source Type: research

Exposure to sub-inhibitory concentrations of gentamicin, ciprofloxacin and cefotaxime induces multidrug resistance and reactive oxygen species generation in meticillin-sensitive Staphylococcus aureus.
CONCLUSIONS: Our study demonstrates that sub-MIC levels of antimicrobials can lead to resistance and cross-resistance across several classes of antibiotics in wild strains of S. aureus, possibly by free radical production. The molecular mechanisms behind the acquisition of drug resistance at low antibiotic concentrations and the specific target genes of reactive oxygen speciesneed to be explored further. PMID: 28598307 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Bhattacharya G, Dey D, Das S, Banerjee A Tags: J Med Microbiol Source Type: research

In vitro activities of vancomycin and linezolid against biofilm-producing methicillin-resistant staphylococci species isolated from catheter-related bloodstream infections from an Egyptian tertiary hospital.
CONCLUSION: Vancomycin, linezolid and rifampicin could be used effectively against methicillin-resistant staphylococci isolated from CRBSIs. PMID: 28598310 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Hashem AA, Abd El Fadeal NM, Shehata AS Tags: J Med Microbiol Source Type: research

In vitro activity of sitafloxacin against Mycobacterium tuberculosis with gyrA/B mutations isolated in Japan.
CONCLUSION: SFX had the lowest cumulative MIC and a relatively low ECOFF value against Mtb, indicating that SFX was not only more effective against gyrA-mutant isolates, but also MDR isolates in Japan. PMID: 28598311 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Yi L, Aono A, Chikamatsu K, Igarashi Y, Yamada H, Takaki A, Mitarai S Tags: J Med Microbiol Source Type: research

Prevalence of faecal carriage of colistin-resistant Gram-negative rods in a university hospital in western France, 2016.
Abstract Plasmid-mediated and chromosomally-encoded colistin resistance is increasingly being reported worldwide. We aimed to determine the prevalence of faecal carriage of colistin-resistant Gram-negative rod isolates in a university hospital in western France. From February to May 2016, rectal swabs from 653 patients hospitalized in various clinical settings were recovered and subsequently screened for colistin resistance using the SuperPolymyxin medium. Antimicrobial susceptibilities were determined according to EUCAST guidelines. Genetic detection of plasmid-mediated colistin resistance was performed by PCR. T...
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Saly M, Jayol A, Poirel L, Megraud F, Nordmann P, Dubois V Tags: J Med Microbiol Source Type: research

How do the epidemiology of paediatric methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus bacteraemia differ?
Abstract PURPOSE: To examine whether the epidemiology of bacteraemia caused by methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) differed in children aged
Source: Journal of Medical Microbiology - June 9, 2017 Category: Microbiology Authors: Abernethy J, Sharland M, Johnson AP, Hope R Tags: J Med Microbiol Source Type: research

Chlorhexidine whole-body washing of patients reduces methicillin-resistant Staphylococcus aureus and has a direct effect on the distribution of the ST5-MRSA-II (New York/Japan) clone.
CONCLUSION: Our findings suggest that the CXG bathing favored the reduction of healthcare-associated MRSA isolates and a temporary reduction of the predominant ST5-MRSA-II (New York/Japan) clone. PMID: 28590235 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: Velázquez-Meza ME, Mendoza-Olazarán S, Echániz-Aviles G, Camacho-Ortiz A, Martínez-Reséndez MF, Valero-Moreno V, Garza-González E Tags: J Med Microbiol Source Type: research

Evaluation of self-collected rectal swabs for the detection of bacteria responsible for sexually transmitted infections in a cohort of HIV-1-infected patients.
CONCLUSION: In conclusion, testing extra-genital sites is crucial for the diagnosis of STIs in men and women presenting or not concomitant urogenital infections in order to detect asymptomatic carriage with the aim of controlling and preventing transmission to their sexual partners. PMID: 28590237 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: Edouard S, Tamalet C, Tissot-Dupont H, Colson P, Ménard A, Ravaux I, Dhiver C, Tomei C, Stein A, Raoult D Tags: J Med Microbiol Source Type: research

What were the risk factors and trends in antimicrobial resistance for enteric fever in London 2005-2012?
Abstract PURPOSE: A study was undertaken to determine the risk factors and trends in antimicrobial resistance for enteric fever. METHODOLOGY: Demographic, antimicrobial susceptibility, typing and epidemiological data were examined for 2005-2012 in patients with enteric fever in London. Single and multivariable logistic regression was used to determine the risk factors associated with antibiotic resistance. RESULTS: 453 cases with Salmonella enterica subsp. enterica serovar Paratyphi A, 17 with S. Paratyphi B and 611 with S. enterica subsp. enterica serovar Typhi were examined. For travellers, 335 (88 %...
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: Dave J, Warburton F, Freedman J, de Pinna E, Grant K, Sefton A, Crawley-Boevey E, Godbole G, Holliman R, Balasegaram S Tags: J Med Microbiol Source Type: research

Detection of Actinomyces spp. in cervical exudates from women with cervical intraepithelial neoplasia or cervical cancer.
CONCLUSION: Patients with cervical cancer had a higher prevalence of the presence of Actinomyces compared to the CIN and control groups. This is the first study in which a deliberate search of this genus has been performed in women with cervical pathologies. The use of specific primers for each species facilitated their detection in comparison with traditional isolation methods. More information is necessary to understand the molecular mechanisms involved in the complex role that bacterial communities may play in the development of cancer (and vice versa). PMID: 28590243 [PubMed - as supplied by publisher] (Source: Jo...
Source: Journal of Medical Microbiology - June 8, 2017 Category: Microbiology Authors: García-García A, Coronel-Martínez J, Leon DC, Romero-Figueroa MDS, Caballero-Pantoja YE, Manzanares-Leal GL, Rodriguez-Morales M, Sandoval-Trujillo H, Ramírez-Durán N Tags: J Med Microbiol Source Type: research

Epidemiology of culture-confirmed infections of Streptococcus pneumoniae (2012-2015) and nasopharyngeal carriage in children and households in Taiwan (2014-2015).
CONCLUSION: The isolation of non-vaccine serotypes and unknown serotypes after the introduction of PCV13 in children highlights the importance of continued surveillance for emerging serotypes. PMID: 28590240 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - June 5, 2017 Category: Microbiology Authors: Janapatla RP, Su LH, Chen HH, Chang HJ, Tsai TC, Chen PY, Chen CL, Chiu CH Tags: J Med Microbiol Source Type: research

Potentially hazardous Streptococcus suis strains latent in asymptomatic pigs in a major swine production area of Thailand.
CONCLUSION: Our results showed the population structure differences between S. suis in central Thailand and other regions; however, zoonotic S. suis is certainly latent in asymptomatic pigs in this intensive swine production area. PMID: 28516843 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 18, 2017 Category: Microbiology Authors: Meekhanon N, Kaewmongkol S, Phimpraphai W, Okura M, Osaki M, Sekizaki T, Takamatsu D Tags: J Med Microbiol Source Type: research

Effects of olanexidine gluconate on preoperative skin preparation: an experimental study in cynomolgus monkeys.
CONCLUSION: The bactericidal effects of olanexidine were comparable to those of commercial antiseptics such as chlorhexidine and povidone-iodine in non-blood-contaminated conditions. More importantly, the effect of olanexidine was hardly affected by blood unlike commercial antiseptics. Thus, it is considered that olanexidine has a favourable property for skin preparation in various types of surgical treatments. PMID: 28516852 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 18, 2017 Category: Microbiology Authors: Nakata H, Tsubotani Y, Nii T, Hagi A, Inoue Y, Imamura T Tags: J Med Microbiol Source Type: research

Recombinant esterase from Corynebacterium pseudotuberculosis in DNA and subunit recombinant vaccines partially protects mice against challenge.
Abstract PURPOSE: We tested the efficacy of the esterase encoded by cp1002_RS09720 from Corynebacteriumpseudotuberculosis in recombinant subunit and DNA caseous lymphadenitis (CLA) vaccines. This target was predicted as one of the best CLA vaccine candidates by mature epitope density analysis. METHODOLOGY: Gene cp1002_RS09720 was cloned into two different vectors (pAE for subunit vaccine and pTARGET for DNA vaccine). Four groups of 15 mice each were immunized with the recombinant esterase rCP09720 associated with aluminium hydroxide adjuvant (G1), pTARGET/cp09720 DNA vaccine (G2), a naked pTARGET (G3) or PBS ...
Source: Journal of Medical Microbiology - May 18, 2017 Category: Microbiology Authors: Brum AA, Rezende AFS, Brilhante FS, Collares T, Begnine K, Seixas FK, Collares TV, Dellagostin OA, Azevedo V, Santos A, Portela RW, Borsuk S Tags: J Med Microbiol Source Type: research

Carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae in HIV-infected children in Zimbabwe.
CONCLUSION: We found a 13.7 % prevalence of ESBL-E carriage in a population where ESBL-E carriage has not been described previously. Antimicrobial resistance (AMR) in Africa merits further study, particularly given the high HIV prevalence and limited diagnostic and therapeutic options available. PMID: 28513417 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 18, 2017 Category: Microbiology Authors: Wilmore SMS, Kranzer K, Williams A, Makamure B, Nhidza AF, Mayini J, Bandason T, Metcalfe J, Nicol MP, Balakrishnan I, Ellington MJ, Woodford N, Hopkins S, McHugh TD, Ferrand RA Tags: J Med Microbiol Source Type: research

Flagellar expression in clinical isolates of non-typeable Haemophilus influenzae.
CONCLUSION: This study provides, for the first time, information on flagellar expression in H. influenzae, representing an important finding related to its evolution and pathogenic potential. PMID: 28513418 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 18, 2017 Category: Microbiology Authors: Carabarin-Lima A, Lozano-Zarain P, Castañeda-Lucio M, Martínez de la Peña CF, Martinez-Garcia J, Flores NL, Cruz EC, González-Posos S, Rocha-Gracia RDC Tags: J Med Microbiol Source Type: research

Histological findings in severe Mycoplasma pneumoniae pneumonia.
PMID: 28513419 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 18, 2017 Category: Microbiology Authors: Miyashita N, Narita M, Tanaka T, Akaike H, Teranishi H, Oishi T, Nakano T, Ouchi K, Okimoto N Tags: J Med Microbiol Source Type: research

Identification of a distinctive phenotype for endocarditis-associated clonal complex 22 MRSA isolates with reduced vancomycin susceptibility.
CONCLUSIONS: The identification of a distinct phenotype for CC22 high V-MIC isolates supports the hypothesis that bacterial factors contribute to the mechanism underlying their association with endocarditis. Further study of these isolates could shed light on the molecular mechanism of endocarditis in humans. PMID: 28504620 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 15, 2017 Category: Microbiology Authors: Marbach H, Boakes E, Lynham S, Ward M, Otter JA, Edgeworth JD Tags: J Med Microbiol Source Type: research

Comparative diagnostic test evaluation of serum procalcitonin and C-reactive protein in suspected bloodstream infections in children with cancer.
CONCLUSION: PCT is a better serological marker for excluding bloodstream infections than CRP. The cut-off value of 0.49 ng ml-1 with a negative predictive value of 94.67 % will be ideal in a clinical setting of immune-compromised children with suspected sepsis. PMID: 28504925 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 15, 2017 Category: Microbiology Authors: R Nath S, Jayapalan S, Nair H, Kusumakumary P, Prema NS, Priyakumari T, Rajamohanan K Tags: J Med Microbiol Source Type: research

Accurate differentiation of Mycobacterium chimaera from Mycobacterium intracellulare by MALDI-TOF MS analysis.
CONCLUSIONS: We have developed a model for the accurate identification of M. chimaera and M. intracellulare by MALDI-TOF MS. This approach has the potential for routine use in microbiology laboratories, as the model itself can be easily implemented into the software of the currently available systems by MALDI-TOF MS manufacturers. PMID: 28504926 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 15, 2017 Category: Microbiology Authors: Pranada AB, Witt E, Bienia M, Kostrzewa M, Timke M Tags: J Med Microbiol Source Type: research

Mechanisms of quinolone action and resistance: where do we stand?
jas G Abstract Quinolone antibiotics represent one of the most important classes of anti-infective agents and, although still clinically valuable, their use has been compromised by the increasing emergence of resistant strains, which has become a prevalent clinical problem. Quinolones act by inhibiting the activity of DNA gyrase and topoisomerase IV - two essential bacterial enzymes that modulate the chromosomal supercoiling required for critical nucleic acid processes. The acquisition of quinolone resistance is recognized to be multifactorial and complex. The main resistance mechanism consists of one or a combina...
Source: Journal of Medical Microbiology - May 12, 2017 Category: Microbiology Authors: Correia S, Poeta P, Hébraud M, Capelo JL, Igrejas G Tags: J Med Microbiol Source Type: research

Molecular study of carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenems and determining their clonal relationship using pulsed-field gel electrophoresis.
CONCLUSIONS: Our results could reflect some hospital multidrug-resistant strains in nosocomial infections. The widespread emergence of carbapenem-resistant isolates has caused increasing concern in recent years. Therefore, specific strategies should be designed and evaluated for the control of resistant strains. PMID: 28485708 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 9, 2017 Category: Microbiology Authors: Shahcheraghi F, Aslani MM, Mahmoudi H, Karimitabar Z, Solgi H, Bahador A, Alikhani MY Tags: J Med Microbiol Source Type: research

Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection.
CONCLUSION: Co-resistance to clinically relevant fluoroquinolone and trimethoprim-sulfamethazole in all UPEC isolates is a cause for concern. These results expand the current knowledge of gene cassettes in both commensal and pathogenic E. coli. PMID: 28485709 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 9, 2017 Category: Microbiology Authors: Oliveira-Pinto C, Diamantino C, Oliveira PL, Reis MP, Costa PS, Paiva MC, Nardi RMD, Magalhães PP, Chartone-Souza E, Nascimento AMA Tags: J Med Microbiol Source Type: research

Epidemiological typing of multidrug-resistant Klebsiella pneumoniae, which causes paediatric ventilator-associated pneumonia in Egypt.
CONCLUSION: As far as we know, this is the first report of the isolation of the same multidrug-resistant (MDR) K. pneumoniae pulsotype from patients and environmental samples in the same hospital ward in Egypt. This study provides a step on the way to understanding the genotyping and epidemiology of MDR K. pneumoniae for enhanced prevention of bacterial transmission. PMID: 28485710 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 9, 2017 Category: Microbiology Authors: Mohamed ER, Aly SA, Halby HM, Ahmed SH, Zakaria AM, El-Asheer OM Tags: J Med Microbiol Source Type: research

Prevalence and species distribution of Achromobacter sp. cultured from cystic fibrosis patients attending the Aarhus centre in Denmark.
erg W PMID: 28481740 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - May 8, 2017 Category: Microbiology Authors: Gade SS, Nørskov-Lauritsen N, Ridderberg W Tags: J Med Microbiol Source Type: research

Assessment of urinary pharmacokinetics and pharmacodynamics of orbifloxacin in healthy dogs with ex vivo modelling.
Abstract PURPOSE: The aim of this study was to investigate the urinary pharmacokinetics (PK) of orbifloxacin (OBFX) administered at 5 mg kg-1 in six healthy dogs. A further aim was to use an ex vivo model to evaluate the urinary PK and pharmacodynamics (PD) of OBFX to determine its urinary bactericidal titre (UBT), which represents the maximal dilution of urine allowing bactericidal activity. METHODOLOGY: Fourteen urinary tract infection (UTI) pathogenic strains of five bacterial species (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis and Staphylococcuspseudintermedi...
Source: Journal of Medical Microbiology - May 4, 2017 Category: Microbiology Authors: Shimizu T, Harada K, Manabe S, Tsukamoto T, Ito N, Hikasa Y Tags: J Med Microbiol Source Type: research

Increase in human immunodeficiency virus 1 diversity and detection of various subtypes and recombinants in north-eastern Brazil.
The objective of this study was to molecularly characterize HIV-1 circulating in Pernambuco, north-eastern Brazil. METHODOLOGY: A total of 64 samples were collected from 2002 to 2003, and another 103 were collected from 2007 to 2009. The protease and partial reverse transcriptase regions of the HIV-1 polymerase-encoding (pol) gene were sequenced, and subtyping, recombination and phylogenetic analyses were performed.Results/Key findings. Subtype B (60.9 %) was found to be predominant, followed by HIV-1 F (31.4 %). Several BF recombinants (4.2 %), and BC and AG recombinants were also identified. The intra-subtype geneti...
Source: Journal of Medical Microbiology - April 21, 2017 Category: Microbiology Authors: Lima K, Leal É, Cavalcanti AMS, Salustiano DM, de Medeiros LB, da Silva SP, Lacerda HR Tags: J Med Microbiol Source Type: research

Rapid detection of Mycobacterium tuberculosis and rifampicin resistance in extrapulmonary tuberculosis and sputum smear-negative pulmonary suspects using Xpert MTB/RIF.
CONCLUSION: The Xpert MTB/RIF assay is a rapid and simple technique with high sensitivity and specificity for diagnosing TB and detecting drug resistance in extrapulmonary and smear-negative TB cases. PMID: 28425873 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 21, 2017 Category: Microbiology Authors: Ullah I, Javaid A, Masud H, Ali M, Basit A, Ahmad W, Younis F, Yasmin R, Khan A, Jabbar A, Husain M, Butt ZA Tags: J Med Microbiol Source Type: research

In vitro and in vivo assessments of Rhodomyrtus tomentosa leaf extract as an alternative anti-streptococcal agent in Nile tilapia (Oreochromis niloticus L.).
Abstract PURPOSE: Rhodomyrtustomentosa is a Thai medicinal plant that has been attracting attention for its remarkable antibacterial properties against Gram-positive pathogenic bacteria. The purpose of this study was to evaluate the antibacterial properties of R. tomentosa leaf extract against Streptococcus agalactiae and Streptococcus iniae isolated from infected tilapia. METHODOLOGY: The anti-streptococcal activity of R. tomentosa was determined using broth microdilution assays. RESULTS: The extract demonstrated strong antibacterial activity against the fish pathogens, with minimum inhibitory concentra...
Source: Journal of Medical Microbiology - April 21, 2017 Category: Microbiology Authors: Na-Phatthalung P, Chusri S, Suanyuk N, Voravuthikunchai SP Tags: J Med Microbiol Source Type: research

Occurrence of qnrB1 and qnrB12 genes, mutation in gyrA and ramR, and expression of efflux pumps in isolates of Klebsiella pneumoniae carriers of blaKPC-2.
CONCLUSION: KPC-2-positive isolates colonizing patients, which also showed qnrB, mutation in gyrA and efflux pumps, may be important reservoirs for disseminating these resistance mechanisms in the hospital environment. PMID: 28425875 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 21, 2017 Category: Microbiology Authors: Scavuzzi AML, Maciel MAV, de Melo HRL, Alves LC, Brayner FA, Lopes ACS Tags: J Med Microbiol Source Type: research

Characterization of the translation elongation factor 1- α gene in a wide range of pathogenic Aspergillus species.
CONCLUSION: The gene was not phylogenetically more informative than other known molecular markers. It will be necessary to test other genes or larger genomic regions to better understand the taxonomy of this important group of fungi. PMID: 28425876 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 21, 2017 Category: Microbiology Authors: Nouripour-Sisakht S, Ahmadi B, Makimura K, Hoog S, Umeda Y, Alshahni MM, Mirhendi H Tags: J Med Microbiol Source Type: research

Origin and distribution of Sporothrix globosa causing sapronoses in Asia.
CONCLUSION: In Asia, S. globosa was the prevalent species. The low diversity of S. globosa suggested a recent divergence with a founder effect of low variability from the variable ancestral species, S. schenckii. PMID: 28327256 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 23, 2017 Category: Microbiology Authors: Moussa TA, Kadasa NM, Al Zahrani HS, Ahmed SA, Feng P, Gerrits van den Ende AH, Zhang Y, Kano R, Li F, Li S, Song Y, Dong B, Rossato L, Dolatabadi S, Hoog S Tags: J Med Microbiol Source Type: research

Novel anti-staphylococcal and anti-biofilm properties of two anti-malarial compounds: MMV665953 {1-(3-chloro-4-fluorophenyl)-3-(3,4-dichlorophenyl)urea} and MMV665807 {5-chloro-2-hydroxy-N-[3-(trifluoromethyl)phenyl]benzamide}.
CONCLUSION: MMV665953 and MMV665807 are more bactericidal against S. aureus biofilms than currently used anti-staphylococcal antibiotics and represent a valuable structural basis for further investigation in the treatment of staphylococcal biofilm-related infections. PMID: 28327271 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 23, 2017 Category: Microbiology Authors: Zapotoczna M, Boksmati N, Donohue S, Bahtiar B, Boland A, Somali HA, Cox A, Humphreys H, O'Gara JP, Brennan M, O'Neill E Tags: J Med Microbiol Source Type: research

Pathogenic potential and genotypic diversity of Campylobacter jejuni: a neglected food-borne pathogen in Brazil.
CONCLUSIONS: In conclusion, the pathogenic potential of the C. jejuni strains studied was highlighted by the high frequency of the majority of the virulence genes searched. The flaA-SVR sequencing and PFGE results showed that some of the strains studied presented a high genotypic similarity, suggesting potential for transmission between animal sources and humans in this country. Altogether, the results characterize further C. jejuni isolates from Brazil, an important producer and exporter of chicken meat. PMID: 28317494 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 20, 2017 Category: Microbiology Authors: Frazão MR, Medeiros MI, Duque SD, Falcão JP Tags: J Med Microbiol Source Type: research

First genetic evidence of Coxiella burnetii in cases presenting with acute febrile illness, India.
PMID: 28319680 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 20, 2017 Category: Microbiology Authors: Kumar S, Gangoliya SR, Alam SI, Patil S, Ajantha GS, Kulkarni RD, Kamboj DV Tags: J Med Microbiol Source Type: research

Determination of antimicrobial resistance of Enterococcus strains isolated from pigs and their genotypic characterization by method of amplification of DNA fragments surrounding rare restriction sites (ADSRRS fingerprinting).
CONCLUSION: Given the possibility of transmission to humans of the high-resistance/multidrug resistance enterococci and the significant role of pigs as food animals in this process, it is necessary to introduce a multilevel control strategy by carrying out research on the resistance and molecular characteristics of indicator bacterial strains isolated from animals on individual farms. PMID: 28260584 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 1, 2017 Category: Microbiology Authors: Nowakiewicz A, Ziółkowska G, Trościańczyk A, Zięba P, Gnat S Tags: J Med Microbiol Source Type: research

Comparison of culture-dependent and culture-independent molecular methods for characterization of vaginal microflora.
CONCLUSION: We found little overlap between the species found using cultivation-dependent and cultivation-independent methods. Rather, culture-dependent and culture-independent methods were found to be complementary in describing the vaginal microflora among South Indian women. Culture-independent methods were found to be superior in detecting clinically relevant vaginal flora. PMID: 28260585 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 1, 2017 Category: Microbiology Authors: Pandya S, Ravi K, Srinivas V, Jadhav S, Khan A, Arun A, Riley LW, Madhivanan P Tags: J Med Microbiol Source Type: research

Molecular epidemiology of Pseudomonas aeruginosa bloodstream infection isolates in a non-outbreak setting.
CONCLUSION: The non-clonal population structure suggests that there is ongoing environmental exposure of inpatients to P. aeruginosa. In clinical areas dealing with at-risk patients, routine attention to mechanism of environmental colonization is important and should be addressed even in the non-outbreak setting. PMID: 28260586 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 1, 2017 Category: Microbiology Authors: McCarthy KL, Kidd TJ, Paterson DL Tags: J Med Microbiol Source Type: research

Antimicrobial-resistant Escherichia coli and Enterococcus spp. isolated from Miranda donkey (Equus asinus): an old problem from a new source with a different approach.
CONCLUSION: To the best of our knowledge, this is the first report showing antibiotic resistance among Escherichiacoli and Enterococcus spp. isolates from the Miranda donkey in Portugal, indicating possible antibiotic-resistant bacterial reservoirs. However, the detection of these resistances presents a low risk for other animals and human beings in that rural area. PMID: 28260587 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 1, 2017 Category: Microbiology Authors: Carvalho I, Campo RD, Sousa M, Silva N, Carrola J, Marinho C, Santos T, Carvalho S, Nóvoa M, Quaresma M, Pereira JE, Cobo M, Igrejas G, Poeta P Tags: J Med Microbiol Source Type: research

Utilization of size polymorphism in ITS1 and ITS2 regions for identification of pathogenic yeast species.
CONCLUSION: FSP method benefits from advantages such as lower cost, higher speed and wider range of species than some commercial yeast-identification methods. We consider this method as one of the easiest molecular approaches for identifying a wide range of human pathogenic yeast species, applicable to both diagnostic and epidemiological purposes. PMID: 28260588 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 1, 2017 Category: Microbiology Authors: Khodadadi H, Karimi L, Jalalizand N, Adin H, Mirhendi H Tags: J Med Microbiol Source Type: research

Improved effect of amikacin-loaded poly(D,L-lactide-co-glycolide) nanoparticles against planktonic and biofilm cells of Pseudomonas aeruginosa.
CONCLUSION: The good antibacterial and antibiofilm activities of A-NPs, in addition to their ability to enter macrophages without any cytotoxicity for these cells, make them a potential candidate to treat P. aeruginosa infections. PMID: 28260589 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 1, 2017 Category: Microbiology Authors: Sabaeifard P, Abdi-Ali A, Gamazo C, Irache JM, Soudi MR Tags: J Med Microbiol Source Type: research

Detection of ISAba1 in association with a novel allelic variant of the β-lactamase ADC-82 and class D β-lactamase genes mediating carbapenem resistance among the clinical isolates of MDR A. baumannii.
CONCLUSION: High incidence of IC-II clones, novel resistance determinants (ADC-82) and elevated resistance mediated by ISAba1 reported here will be of enormous importance while assessing the emergence of extremely resistant A. baumannii in India. PMID: 28260590 [PubMed - in process] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - March 1, 2017 Category: Microbiology Authors: Saranathan R, Kumari R, Kalaivani R, Suresh S, Rani A, Purty S, Prashanth K Tags: J Med Microbiol Source Type: research

Resistotyping, phenotyping and genotyping of New Delhi metallo- β-lactamase (NDM) among gram negative bacilli from Iranian patients.
CONCLUSION: Here, high levels of antibiotic resistance and high frequency of MDR and XDR profiles as well as high prevalence of blaNDM-1 in investigated bacteria. PMID: 28150578 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 1, 2017 Category: Microbiology Authors: Shokri D, Rabbani Khorasgani M, Fatemi SM, Soleimani-Delfan A Tags: J Med Microbiol Source Type: research

Biocompatible, biodegradable polymeric antibacterial nanoparticles for enhancing the effects of the third generation cephalosporin against resistant bacteria.
CONCLUSION: The synergistic effect of ceftriaxone loaded CNPs can be useful finding for treatment of MRSA and E. coli infections. PMID: 28150580 [PubMed - as supplied by publisher] (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - February 1, 2017 Category: Microbiology Authors: Mushtaq S, Khan JA, Rabbani F, Latif U, Arfan M, Yameen MA Tags: J Med Microbiol Source Type: research