Proteomic Analysis of Non-human Primate Peripheral Blood Mononuclear Cells During Burkholderia mallei Infection Reveals a Role of Ezrin in Glanders Pathogenesis
Burkholderia mallei, the causative agent of glanders, is a gram-negative intracellular bacterium. Depending on different routes of infection, the disease is manifested by pneumonia, septicemia, and chronic infections of the skin. B. mallei poses a serious biological threat due to its ability to infect via aerosol route, resistance to multiple antibiotics and to date there are no US Food and Drug Administration (FDA) approved vaccines available. Induction of innate immunity, inflammatory cytokines and chemokines following B. mallei infection, have been observed in in vitro and small rodent models; however, a global characte...
Source: Frontiers in Microbiology - April 22, 2021 Category: Microbiology Source Type: research
< em > In Vitro < /em > and < em > In Vivo < /em > Characterization of Tebipenem (TBP), an Orally Active Carbapenem, against Biothreat Pathogens
Antimicrob Agents Chemother. 2021 Feb 16:AAC.02385-20. doi: 10.1128/AAC.02385-20. Online ahead of print.ABSTRACTBacillus anthracis and Yersinia pestis, causative pathogens for anthrax and plague, respectively, along with Burkholderia mallei and B. pseudomallei are potential bioterrorism threats. Tebipenem pivoxil hydrobromide (TBP HBr, formerly SPR994), is an orally available prodrug of tebipenem, a carbapenem with activity versus multidrug-resistant (MDR) gram-negative pathogens, including quinolone-resistant and extended-spectrum-β-lactamase-producing Enterobacterales. We evaluated the in vitro activity and in vivo effi...
Source: Antimicrobial Agents and Chemotherapy - February 17, 2021 Category: Microbiology Authors: Nicholas P Clayton Akash Jain Stephanie A Halasohoris Lisa M Pysz Sanae Lembirik Steven D Zumbrun Christopher D Kane Michael J Hackett Denise Pfefferle M Autumn Smiley Michael S Anderson Henry Heine Gabriel T Meister Michael J Pucci Source Type: research
In Vitro and In Vivo Characterization of Tebipenem (TBP), an Orally Active Carbapenem, against Biothreat Pathogens.
Abstract
Bacillus anthracis and Yersinia pestis, causative pathogens for anthrax and plague, respectively, along with Burkholderia mallei and B. pseudomallei are potential bioterrorism threats. Tebipenem pivoxil hydrobromide (TBP HBr, formerly SPR994), is an orally available prodrug of tebipenem, a carbapenem with activity versus multidrug-resistant (MDR) gram-negative pathogens, including quinolone-resistant and extended-spectrum-β-lactamase-producing Enterobacterales. We evaluated the in vitro activity and in vivo efficacy of tebipenem against biothreat pathogens. Tebipenem was active in vitro against 3...
Source: Antimicrobial Agents and Chemotherapy - February 16, 2021 Category: Microbiology Authors: Clayton NP, Jain A, Halasohoris SA, Pysz LM, Lembirik S, Zumbrun SD, Kane CD, Hackett MJ, Pfefferle D, Smiley MA, Anderson MS, Heine H, Meister GT, Pucci MJ Tags: Antimicrob Agents Chemother Source Type: research
A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections
Volume 12, Issue 1, December 2021, Page 493-506 . (Source: Virulence)
Source: Virulence - January 29, 2021 Category: Virology Authors: Guanbo Wang Lena Glaser Nichollas E. Scott Yasmine Fathy Mohamed Rebecca Ingram Karine Laroucau Miguel A. Valvano Source Type: research
In vitro antibacterial activity and in vivo efficacy of sulbactam-durlobactam against pathogenic Burkholderia species.
Abstract
The Gram-negative bacterial genus Burkholderia includes several hard-to-treat human pathogens: two biothreat species, B. mallei (causing glanders) and B. pseudomallei (causing melioidosis), and the B. cepacia complex (BCC) and B. gladioli, which cause chronic lung infections in persons with cystic fibrosis. All Burkholderia spp possess an Ambler class A Pen β-lactamase, which confers resistance to β-lactams. The β-lactam-β-lactamase inhibitor combination sulbactam-durlobactam (SUL-DUR) is in clinical development for the treatment of Acinetobacter infections. Herein, we evaluated SUL-DUR for in...
Source: Antimicrobial Agents and Chemotherapy - December 14, 2020 Category: Microbiology Authors: Papp-Wallace KM, Shapiro AB, Becka SA, Zeiser ET, LiPuma JJ, Lane DJ, Panchal RG, Mueller JP, O'Donnell JP, Miller AA Tags: Antimicrob Agents Chemother Source Type: research
Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories.
Abstract
The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories....
Source: Emerging Infectious Diseases - October 21, 2020 Category: Infectious Diseases Authors: Kesterson AE, Craig JE, Chuvala LJ, Heine HS Tags: Emerg Infect Dis Source Type: research
Secondary metabolites from the Burkholderia pseudomallei complex: structure, ecology, and evolution.
Abstract
Bacterial secondary metabolites play important roles in promoting survival, though few have been carefully studied in their natural context. Numerous gene clusters code for secondary metabolites in the genomes of members of the Bptm group, made up of three closely related species with distinctly different lifestyles: the opportunistic pathogen Burkholderia pseudomallei, the non-pathogenic saprophyte Burkholderia thailandensis, and the host-adapted pathogen Burkholderia mallei. Several biosynthetic gene clusters are conserved across two or all three species, and this provides an opportunity to unde...
Source: Journal of Industrial Microbiology and Biotechnology - October 14, 2020 Category: Microbiology Authors: Klaus JR, Coulon PML, Koirala P, Seyedsayamdost MR, Déziel E, Chandler JR Tags: J Ind Microbiol Biotechnol Source Type: research
The Peptidoglycan-associated lipoprotein Pal contributes to the virulence of Burkholderia mallei and provides protection against lethal aerosol challenge
Volume 11, Issue 1, December 2020, Page 1024-1040 . (Source: Virulence)
Source: Virulence - August 14, 2020 Category: Virology Authors: Jeremy S. Dyke Maria Cristina Huertas-Diaz Frank Michel Nathan E. Holladay Robert J. Hogan Biao He Eric R. Lafontaine Source Type: research
Clinical repercussions of Glanders (Burkholderia mallei infection) in a Brazilian child: a case report
We report the case of an 11-year-old boy who was presented to an emergency department with chest pain and dyspnea. He evolved into septic shock, pneumonia, and multiple abscesses. B. mallei was found in the exudate culture. Human infection is rare and difficult to confirm. The knowledge on glanders is important for differential diagnosis from other serious illnesses causing pneumonia and multiple abscesses. (Source: Revista da Sociedade Brasileira de Medicina Tropical)
Source: Revista da Sociedade Brasileira de Medicina Tropical - June 22, 2020 Category: Tropical Medicine Source Type: research
The Burkholderia thailandensis Phages ΦE058 and ΦE067 Represent Distinct Prototypes of a New Subgroup of Temperate Burkholderia Myoviruses
In this study we isolated two phages from B. pseudomallei and three phages from B. thailandensis and determined their morphology, host range, and relationship. All five phages belong to the family Myoviridae, but some of them revealed different host specificities. DNA-DNA hybridization experiments indicated that the phages belong to two groups. One group, composed of ΦE058 (44,121 bp) and ΦE067 (43,649 bp), represents a new subgroup of Burkholderia myoviruses that is not related to known phages. The genomes of ΦE058 and ΦE067 are similar but also show some striking differences. Repressor proteins differ clearly allowin...
Source: Frontiers in Microbiology - May 26, 2020 Category: Microbiology Source Type: research
Development of a microsphere-based immunoassay for the serological detection of glanders in equids.
Abstract
Burkholderia mallei is the etiologic agent of glanders, an infectious disease of solipeds, with renewed scientific interest due to its increasing incidence in different parts of the world. More rapid, sensitive and specific assays are required by laboratories for confirmatory testing of this disease. A microsphere-based immunoassay consisting of beads coated with B. mallei recombinant proteins (BimA, GroEL, Hcp1, and TssB) has been developed for the serological diagnosis of glanders. The proteins' performance was compared with the OIE reference complement fixation test (CFT) and an indirect enzyme...
Source: Acta Tropica - April 13, 2020 Category: Infectious Diseases Authors: Laroucau K, Saqib M, Martin B, Deshayes T, Bertin C, Wernery U, Joseph S, Singha H, Tripathi BN, Beck C Tags: Acta Trop Source Type: research
Combinations of early generation antibiotics and antimicrobial peptides are effective against a broad spectrum of bacterial biothreat agents
In this study, the therapeutic potential of combinations of an antibiotic and a broad-spectrum antimicrobial peptide (AMP) was evaluated against five bacterial biothreats, the etiologic agents of glanders (Burkholderia mallei), melioidosis (Burkholderia pseudomallei), plague (Yersinia pestis), tularemia (Francisella tularensis), and anthrax (Bacillus anthracis). The therapeutics included licensed early generation antibiotics which are now rarely used. Three antibiotics and one 24- amino acid AMP were selected based on MIC assay data. Combinations of the AMP and tigecycline, minocycline, or novobiocin were screened for syne...
Source: Microbial Pathogenesis - February 9, 2020 Category: Infectious Diseases Source Type: research
Virulence from the rhizosphere: ecology and evolution of Burkholderia pseudomallei-complex species.
Abstract
Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm) cause the often-lethal infectious diseases melioidosis and glanders, respectively. Curiously, closely related species within the Bp complex share a nearly identical arsenal of virulence traits, yet are harmless to humans. Clues to the origin of virulence in this group can be found in their genetics and ecology. As a resident of the rhizosphere, Bp faces competition for nutrients and predation by other organisms. Adaptation over millennia has enabled Bp to accumulate mechanisms that overlap in their ability to promote fitness in the enviro...
Source: Current Opinion in Microbiology - February 2, 2020 Category: Microbiology Authors: French CT, Bulterys PL, Woodward CL, Tatters AO, Ng KR, Miller JF Tags: Curr Opin Microbiol Source Type: research
[ASAP] Synthesis of the Oligosaccharides of < italic toggle="yes" > Burkholderia pseudomallei < /italic > and < italic toggle="yes" > B. mallei < /italic > Capsular Polysaccharide and Preliminary Immunological Studies of Their Protein Conjugates
The Journal of Organic ChemistryDOI: 10.1021/acs.joc.9b03085 (Source: The Journal of Organic Chemistry)
Source: The Journal of Organic Chemistry - January 22, 2020 Category: Chemistry Authors: Xueyun Geng †, Guirong Wang†, Zhongwu Guo‡, and Guofeng Gu*† Source Type: research
