Potential of recombinant LiHyQ, a novel Leishmania infantum protein, for the diagnosis of canine visceral leishmaniasis and as a diagnostic and prognostic marker for human leishmaniasis and human immunodeficiency virus co-infection: a preliminary study
In conclusion, our preliminary data suggest a diagnostic and prognostic role for rLiHyQ against leishmaniasis.PMID:34537185 | DOI:10.1016/j.actatropica.2021.106126 (Source: Acta Tropica)
Source: Acta Tropica - September 19, 2021 Category: Infectious Diseases Authors: Tha ís T O Santos Fernanda F Ramos Isabela A P Gon çalves Grasiele S V Tavares Fernanda Ludolf Raquel S Bandeira Alessandra M Silva Jo ão A Oliveira-da-Silva Thiago A R Reis Amanda S Machado Daniela P Lage Camila S Freitas Danniele L Vale V ívian T Ma Source Type: research

Potential of recombinant LiHyQ, a novel Leishmania infantum protein, for the diagnosis of canine visceral leishmaniasis and as a diagnostic and prognostic marker for human leishmaniasis and human immunodeficiency virus co-infection: a preliminary study
In conclusion, our preliminary data suggest a diagnostic and prognostic role for rLiHyQ against leishmaniasis.PMID:34537185 | DOI:10.1016/j.actatropica.2021.106126 (Source: Acta Tropica)
Source: Acta Tropica - September 19, 2021 Category: Infectious Diseases Authors: Tha ís T O Santos Fernanda F Ramos Isabela A P Gon çalves Grasiele S V Tavares Fernanda Ludolf Raquel S Bandeira Alessandra M Silva Jo ão A Oliveira-da-Silva Thiago A R Reis Amanda S Machado Daniela P Lage Camila S Freitas Danniele L Vale V ívian T Ma Source Type: research

Antioxidant and antiviral activity of fullerol against Zika virus
In this study, fullerol did not alter cell viability at the concentrations tested, proving to be inert, beyond to presenting high antioxidant power at low concentrations. ZIKV infection of human glioblastoma increased the production of reactive oxygen species by 60% and modulated the Nrf-2 pathway activity negatively. After treatment with fullerol, both conditions were restored to baseline levels. Additionally, fullerol was able to reduce viral production by up to 90%. Therefore, our results suggest that fullerol as a promising candidate in the control of ZIKV infections, presenting both antioxidant and antiviral action.PM...
Source: Acta Tropica - September 18, 2021 Category: Infectious Diseases Authors: Samille Henriques Pereira Let ícia Trindade Almeida Ariane Coelho Ferraz Marina de Souza Ladeira Luiz Orlando Ladeira C íntia Lopes de Brito Magalhães Breno de Mello Silva Source Type: research

Catch me if you can: Under-detection of Trypanosoma cruzi (Kinetoplastea: Trypanosomatida) infections in Triatoma dimidiata s.l. (Hemiptera: Reduviidae) from Central America
In this study we compared six assays for detecting the Chagas parasite, Trypanosoma cruzi: genomic reduced representation sequencing (here referred to as genotype-by-sequencing or GBS), two with conventional PCR (i.e., agarose gel detection), two with qPCR, and microscopy. Our results show that, compared to GBS genomic analysis, microscopy and PCR under-detected T. cruzi in vectors from Central America. Of 94 samples, 44% (50/94) were positive based on genomic analysis. The lowest detection, 9% (3/32) was in a subset assayed with microscopy. Four PCR assays, two with conventional PCR and two with qPCR showed intermediate l...
Source: Acta Tropica - September 18, 2021 Category: Infectious Diseases Authors: Lori Stevens Raquel Asunci ón Lima-Cordón Sara Helms Cahan Patricia Dorn Carlota Monroy Heather J Axen Andrew Nguyen Yainna Hern áiz-Hernánde Antonieta Rodas Silvia A Justi Source Type: research

Current status of human liver fluke infections in the Greater Mekong Subregion
Acta Trop. 2021 Sep 10;224:106133. doi: 10.1016/j.actatropica.2021.106133. Online ahead of print.ABSTRACTThe Greater Mekong Subregion (GMS) is a trans-national region of the Mekong River basin in Southeast Asia comprising Cambodia, the People's Republic of China (specifically Yunnan Province and Guangxi Zhuang Autonomous Region), Lao People's Democratic Republic (Lao PDR), Myanmar, Thailand, and Vietnam home to more than 340 million people or almost 4.5% of the global population. Human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis have been major public health problems in this region for d...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Banchob Sripa Apiporn T Suwannatrai Somphou Sayasone Dung Trung Do Virak Khieu Yichao Yang Source Type: research

Role of two aminoacyl-tRNA synthetase associated proteins (Endothelial Monocyte Activating Polypeptides 1 and 2) of Leishmania donovani in chemotaxis of human monocytes
Acta Trop. 2021 Sep 9:106128. doi: 10.1016/j.actatropica.2021.106128. Online ahead of print.ABSTRACTVisceral leishmaniasis is caused by the protozoan parasite Leishmania donovani. It is a fatal form of leishmaniasis prevalent in Indian subcontinent. Since there are no human licensed vaccines available for leishmaniasis, chemotherapeutic drugs remain the only means for combating parasitic infections. We have earlier identified a total of 26 amino-acyl tRNA synthetases (aaRS) along with five stand-alone editing domains and two aaRS-associated proteins in Leishmania donovani. In addition to their canonical role of tRNA aminoa...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Smriti Tandon Rohini Muthuswami Rentala Madhubala Source Type: research

Oestrid myiasis at a cross-road
Acta Trop. 2021 Sep 9:106131. doi: 10.1016/j.actatropica.2021.106131. Online ahead of print.ABSTRACTThe oestrids are a genuinely unique group of Diptera that represent many species whose larvae parasitize mammals, feeding on live tissues of a wide range of hosts, from mice to elephants, causing obligatory myiases. Those have had greatest impact on the activities of humans and their use of animals (e.g., as food, recreation, in the conduct of wars). To a large extent their impact has been controlled through the use of therapeutic application of synthetic chemical treatments. While in some limited cases eradication has been ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Douglas D Colwell Domenico Otranto Source Type: research

Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China
This study determined the prevalence of T. gondii, and its genotypes, in pork. A total of 1,223 diaphragm muscle samples were collected from eight slaughterhouses and 79 markets and detected by PCR amplification of the ITS gene. All of the positive samples were used for genotype identification by PCR-RFLP with 11 genetic markers. The total positive rate of T. gondii in Chongqing pork was 8.7%, and differences in T. gondii infection rates were found between different districts (0%-23.3%), seasons (e.g., 4.3% from Spring, 7.3% from Summer, 11.4% from Autumn, 12.0% from Winter) and years (2.7%-14.3%). Six samples were success...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Yehua Zhang Jianhua Xie Rongsheng Mi Hongquan Ling Lu Luo Haiyan Jia Xiaoli Zhang Yan Huang Haiyan Gong Xiangan Han Zheng Zeng Zhaoguo Chen Source Type: research

Spatial analysis of areas at risk for schistosomiasis in the Alto Tiet ê Basin, São Paulo, Brazil
Acta Trop. 2021 Sep 9:106132. doi: 10.1016/j.actatropica.2021.106132. Online ahead of print.ABSTRACTThe present study identified locations with the presence of Biomphalaria and the areas at risk for Schistosoma mansoni, the etiological agent of schistosomiasis, in the Metropolitan Region of São Paulo (MRSP), located in the Alto Tietê Basin (ATB), São Paulo, Brazil. The study area comprises floodplains of the Tietê River from its source, in Salesópolis, to Rasgão Dam, in Pirapora do Bom Jesus, covering 39 municipalities. Data from the planorbidic chart of the malacology laboratory of ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Raquel Gardini Sanches Palasio Thays de Jesus Rossignoli Riccardo Costabile Simone Di Sessa Fernanda Pires Ohlweiler Francisco Chiaravalloti-Neto Source Type: research

Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation
This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future stud...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Nilton Barnab é Rodrigues Lu ís Eduardo Martinez Villegas Ana Paula Marques Duarte Alessandra Silva Orfan ó Breno Dos Anjos Costa Rafael Nacif Pimenta Marcus V G Lacerda Wuelton M Monteiro N ágila Francinete Costa Secundino Paulo Filemon Paolucci Pime Source Type: research

Host DNA repair response to oxidative damage is modulated by Trypanosoma cruzi in a strain-dependent manner
Acta Trop. 2021 Sep 9:106127. doi: 10.1016/j.actatropica.2021.106127. Online ahead of print.ABSTRACTThe conservation of genomic integrity and stability is essential for cell survival. DNA Damage Responses (DDRs) are considered of paramount importance for all living beings and involve mechanisms of cell cycle regulation and damage-specific DNA repair pathways. Hydrogen peroxide (H2O2) is a compound that, in supraphysiological concentrations, damages biomolecules including the DNA, causing base modifications and strand breaks. There is evidence that Trypanosoma cruzi, the protozoan that causes Chagas disease, interferes in t...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Ester Rose Aline Moraes Tatiana Shiroma Nadjar Nitz Ana de C ássia Rosa Riccardo Pratesi Luciana Hagstr öm Juliana Lott de Carvalho Mariana Hecht Source Type: research

Current status of human liver fluke infections in the Greater Mekong Subregion
Acta Trop. 2021 Sep 10;224:106133. doi: 10.1016/j.actatropica.2021.106133. Online ahead of print.ABSTRACTThe Greater Mekong Subregion (GMS) is a trans-national region of the Mekong River basin in Southeast Asia comprising Cambodia, the People's Republic of China (specifically Yunnan Province and Guangxi Zhuang Autonomous Region), Lao People's Democratic Republic (Lao PDR), Myanmar, Thailand, and Vietnam home to more than 340 million people or almost 4.5% of the global population. Human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis have been major public health problems in this region for d...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Banchob Sripa Apiporn T Suwannatrai Somphou Sayasone Dung Trung Do Virak Khieu Yichao Yang Source Type: research

Role of two aminoacyl-tRNA synthetase associated proteins (Endothelial Monocyte Activating Polypeptides 1 and 2) of Leishmania donovani in chemotaxis of human monocytes
Acta Trop. 2021 Sep 9:106128. doi: 10.1016/j.actatropica.2021.106128. Online ahead of print.ABSTRACTVisceral leishmaniasis is caused by the protozoan parasite Leishmania donovani. It is a fatal form of leishmaniasis prevalent in Indian subcontinent. Since there are no human licensed vaccines available for leishmaniasis, chemotherapeutic drugs remain the only means for combating parasitic infections. We have earlier identified a total of 26 amino-acyl tRNA synthetases (aaRS) along with five stand-alone editing domains and two aaRS-associated proteins in Leishmania donovani. In addition to their canonical role of tRNA aminoa...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Smriti Tandon Rohini Muthuswami Rentala Madhubala Source Type: research

Oestrid myiasis at a cross-road
Acta Trop. 2021 Sep 9:106131. doi: 10.1016/j.actatropica.2021.106131. Online ahead of print.ABSTRACTThe oestrids are a genuinely unique group of Diptera that represent many species whose larvae parasitize mammals, feeding on live tissues of a wide range of hosts, from mice to elephants, causing obligatory myiases. Those have had greatest impact on the activities of humans and their use of animals (e.g., as food, recreation, in the conduct of wars). To a large extent their impact has been controlled through the use of therapeutic application of synthetic chemical treatments. While in some limited cases eradication has been ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Douglas D Colwell Domenico Otranto Source Type: research

Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China
This study determined the prevalence of T. gondii, and its genotypes, in pork. A total of 1,223 diaphragm muscle samples were collected from eight slaughterhouses and 79 markets and detected by PCR amplification of the ITS gene. All of the positive samples were used for genotype identification by PCR-RFLP with 11 genetic markers. The total positive rate of T. gondii in Chongqing pork was 8.7%, and differences in T. gondii infection rates were found between different districts (0%-23.3%), seasons (e.g., 4.3% from Spring, 7.3% from Summer, 11.4% from Autumn, 12.0% from Winter) and years (2.7%-14.3%). Six samples were success...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Yehua Zhang Jianhua Xie Rongsheng Mi Hongquan Ling Lu Luo Haiyan Jia Xiaoli Zhang Yan Huang Haiyan Gong Xiangan Han Zheng Zeng Zhaoguo Chen Source Type: research

Spatial analysis of areas at risk for schistosomiasis in the Alto Tiet ê Basin, São Paulo, Brazil
Acta Trop. 2021 Sep 9:106132. doi: 10.1016/j.actatropica.2021.106132. Online ahead of print.ABSTRACTThe present study identified locations with the presence of Biomphalaria and the areas at risk for Schistosoma mansoni, the etiological agent of schistosomiasis, in the Metropolitan Region of São Paulo (MRSP), located in the Alto Tietê Basin (ATB), São Paulo, Brazil. The study area comprises floodplains of the Tietê River from its source, in Salesópolis, to Rasgão Dam, in Pirapora do Bom Jesus, covering 39 municipalities. Data from the planorbidic chart of the malacology laboratory of ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Raquel Gardini Sanches Palasio Thays de Jesus Rossignoli Riccardo Costabile Simone Di Sessa Fernanda Pires Ohlweiler Francisco Chiaravalloti-Neto Source Type: research

Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation
This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future stud...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Nilton Barnab é Rodrigues Lu ís Eduardo Martinez Villegas Ana Paula Marques Duarte Alessandra Silva Orfan ó Breno Dos Anjos Costa Rafael Nacif Pimenta Marcus V G Lacerda Wuelton M Monteiro N ágila Francinete Costa Secundino Paulo Filemon Paolucci Pime Source Type: research

Host DNA repair response to oxidative damage is modulated by Trypanosoma cruzi in a strain-dependent manner
Acta Trop. 2021 Sep 9:106127. doi: 10.1016/j.actatropica.2021.106127. Online ahead of print.ABSTRACTThe conservation of genomic integrity and stability is essential for cell survival. DNA Damage Responses (DDRs) are considered of paramount importance for all living beings and involve mechanisms of cell cycle regulation and damage-specific DNA repair pathways. Hydrogen peroxide (H2O2) is a compound that, in supraphysiological concentrations, damages biomolecules including the DNA, causing base modifications and strand breaks. There is evidence that Trypanosoma cruzi, the protozoan that causes Chagas disease, interferes in t...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Ester Rose Aline Moraes Tatiana Shiroma Nadjar Nitz Ana de C ássia Rosa Riccardo Pratesi Luciana Hagstr öm Juliana Lott de Carvalho Mariana Hecht Source Type: research

Current status of human liver fluke infections in the Greater Mekong Subregion
Acta Trop. 2021 Sep 9:106133. doi: 10.1016/j.actatropica.2021.106133. Online ahead of print.ABSTRACTThe Greater Mekong Subregion (GMS) is a trans-national region of the Mekong River basin in Southeast Asia comprising Cambodia, the People's Republic of China (specifically Yunnan Province and Guangxi Zhuang Autonomous Region), Lao People's Democratic Republic (Lao PDR), Myanmar, Thailand, and Vietnam home to more than 340 million people or almost 4.5% of the global population. Human liver fluke infections caused by O. viverrini and C. sinensis have been major public health problems in this region for decades. Opisthorchiasis...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Banchob Sripa Apiporn T Suwannatrai Somphou Sayasone Do Dung Trung Virak Khieu Yichao Yang Source Type: research

Role of two aminoacyl-tRNA synthetase associated proteins (Endothelial Monocyte Activating Polypeptides 1 and 2) of Leishmania donovani in chemotaxis of human monocytes
Acta Trop. 2021 Sep 9:106128. doi: 10.1016/j.actatropica.2021.106128. Online ahead of print.ABSTRACTVisceral leishmaniasis is caused by the protozoan parasite Leishmania donovani. It is a fatal form of leishmaniasis prevalent in Indian subcontinent. Since there are no human licensed vaccines available for leishmaniasis, chemotherapeutic drugs remain the only means for combating parasitic infections. We have earlier identified a total of 26 amino-acyl tRNA synthetases (aaRS) along with five stand-alone editing domains and two aaRS-associated proteins in Leishmania donovani. In addition to their canonical role of tRNA aminoa...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Smriti Tandon Rohini Muthuswami Rentala Madhubala Source Type: research

Oestrid myiasis at a cross-road
Acta Trop. 2021 Sep 9:106131. doi: 10.1016/j.actatropica.2021.106131. Online ahead of print.ABSTRACTThe oestrids are a genuinely unique group of Diptera that represent many species whose larvae parasitize mammals, feeding on live tissues of a wide range of hosts, from mice to elephants, causing obligatory myiases. Those have had greatest impact on the activities of humans and their use of animals (e.g., as food, recreation, in the conduct of wars). To a large extent their impact has been controlled through the use of therapeutic application of synthetic chemical treatments. While in some limited cases eradication has been ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Douglas D Colwell Domenico Otranto Source Type: research

Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China
This study determined the prevalence of T. gondii, and its genotypes, in pork. A total of 1,223 diaphragm muscle samples were collected from eight slaughterhouses and 79 markets and detected by PCR amplification of the ITS gene. All of the positive samples were used for genotype identification by PCR-RFLP with 11 genetic markers. The total positive rate of T. gondii in Chongqing pork was 8.7%, and differences in T. gondii infection rates were found between different districts (0%-23.3%), seasons (e.g., 4.3% from Spring, 7.3% from Summer, 11.4% from Autumn, 12.0% from Winter) and years (2.7%- 14.3%). Six samples were succes...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Yehua Zhang Jianhua Xie Rongsheng Mi Hongquan Ling Lu Luo Haiyan Jia Xiaoli Zhang Yan Huang Haiyan Gong Xiangan Han Zheng Zeng Zhaoguo Chen Source Type: research

Spatial analysis of areas at risk for schistosomiasis in the Alto Tiet ê Basin, São Paulo, Brazil
Acta Trop. 2021 Sep 9:106132. doi: 10.1016/j.actatropica.2021.106132. Online ahead of print.ABSTRACTThe present study identified locations with the presence of Biomphalaria and the areas at risk for Schistosoma mansoni, the etiological agent of schistosomiasis, in the Metropolitan Region of São Paulo (MRSP), located in the Alto Tietê Basin (ATB), São Paulo, Brazil. The study area comprises floodplains of the Tietê River from its source, in Salesópolis, to Rasgão Dam, in Pirapora do Bom Jesus, covering 39 municipalities. Data from the planorbidic chart of the malacology laboratory of ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Raquel Gardini Sanches Palasio Thays de Jesus Rossignoli Riccardo Costabile Simone Di Sessa Fernanda Pires Ohlweiler Francisco Chiaravalloti-Neto Source Type: research

Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation
This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future stud...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Nilton Barnab é Rodrigues Lu ís Eduardo Martinez Villegas Ana Paula Marques Duarte Alessandra Silva Orfan ó Breno Dos Anjos Costa Rafael Nacif Pimenta Marcus V G Lacerda Wuelton M Monteiro N ágila Francinete Costa Secundino Paulo Filemon Paolucci Pime Source Type: research

Host DNA repair response to oxidative damage is modulated by Trypanosoma cruzi in a strain-dependent manner
Acta Trop. 2021 Sep 9:106127. doi: 10.1016/j.actatropica.2021.106127. Online ahead of print.ABSTRACTThe conservation of genomic integrity and stability is essential for cell survival. DNA Damage Responses (DDRs) are considered of paramount importance for all living beings and involve mechanisms of cell cycle regulation and damage-specific DNA repair pathways. Hydrogen peroxide (H2O2) is a compound that, in supraphysiological concentrations, damages biomolecules including the DNA, causing base modifications and strand breaks. There is evidence that Trypanosoma cruzi, the protozoan that causes Chagas disease, interferes in t...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Ester Rose Aline Moraes Tatiana Shiroma Nadjar Nitz Ana de C ássia Rosa Riccardo Pratesi Luciana Hagstr öm Juliana Lott de Carvalho Mariana Hecht Source Type: research

Current status of human liver fluke infections in the Greater Mekong Subregion
Acta Trop. 2021 Sep 9:106133. doi: 10.1016/j.actatropica.2021.106133. Online ahead of print.ABSTRACTThe Greater Mekong Subregion (GMS) is a trans-national region of the Mekong River basin in Southeast Asia comprising Cambodia, the People's Republic of China (specifically Yunnan Province and Guangxi Zhuang Autonomous Region), Lao People's Democratic Republic (Lao PDR), Myanmar, Thailand, and Vietnam home to more than 340 million people or almost 4.5% of the global population. Human liver fluke infections caused by O. viverrini and C. sinensis have been major public health problems in this region for decades. Opisthorchiasis...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Banchob Sripa Apiporn T Suwannatrai Somphou Sayasone Do Dung Trung Virak Khieu Yichao Yang Source Type: research

Role of two aminoacyl-tRNA synthetase associated proteins (Endothelial Monocyte Activating Polypeptides 1 and 2) of Leishmania donovani in chemotaxis of human monocytes
Acta Trop. 2021 Sep 9:106128. doi: 10.1016/j.actatropica.2021.106128. Online ahead of print.ABSTRACTVisceral leishmaniasis is caused by the protozoan parasite Leishmania donovani. It is a fatal form of leishmaniasis prevalent in Indian subcontinent. Since there are no human licensed vaccines available for leishmaniasis, chemotherapeutic drugs remain the only means for combating parasitic infections. We have earlier identified a total of 26 amino-acyl tRNA synthetases (aaRS) along with five stand-alone editing domains and two aaRS-associated proteins in Leishmania donovani. In addition to their canonical role of tRNA aminoa...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Smriti Tandon Rohini Muthuswami Rentala Madhubala Source Type: research

Oestrid myiasis at a cross-road
Acta Trop. 2021 Sep 9:106131. doi: 10.1016/j.actatropica.2021.106131. Online ahead of print.ABSTRACTThe oestrids are a genuinely unique group of Diptera that represent many species whose larvae parasitize mammals, feeding on live tissues of a wide range of hosts, from mice to elephants, causing obligatory myiases. Those have had greatest impact on the activities of humans and their use of animals (e.g., as food, recreation, in the conduct of wars). To a large extent their impact has been controlled through the use of therapeutic application of synthetic chemical treatments. While in some limited cases eradication has been ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Douglas D Colwell Domenico Otranto Source Type: research

Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China
This study determined the prevalence of T. gondii, and its genotypes, in pork. A total of 1,223 diaphragm muscle samples were collected from eight slaughterhouses and 79 markets and detected by PCR amplification of the ITS gene. All of the positive samples were used for genotype identification by PCR-RFLP with 11 genetic markers. The total positive rate of T. gondii in Chongqing pork was 8.7%, and differences in T. gondii infection rates were found between different districts (0%-23.3%), seasons (e.g., 4.3% from Spring, 7.3% from Summer, 11.4% from Autumn, 12.0% from Winter) and years (2.7%- 14.3%). Six samples were succes...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Yehua Zhang Jianhua Xie Rongsheng Mi Hongquan Ling Lu Luo Haiyan Jia Xiaoli Zhang Yan Huang Haiyan Gong Xiangan Han Zheng Zeng Zhaoguo Chen Source Type: research

Spatial analysis of areas at risk for schistosomiasis in the Alto Tiet ê Basin, São Paulo, Brazil
Acta Trop. 2021 Sep 9:106132. doi: 10.1016/j.actatropica.2021.106132. Online ahead of print.ABSTRACTThe present study identified locations with the presence of Biomphalaria and the areas at risk for Schistosoma mansoni, the etiological agent of schistosomiasis, in the Metropolitan Region of São Paulo (MRSP), located in the Alto Tietê Basin (ATB), São Paulo, Brazil. The study area comprises floodplains of the Tietê River from its source, in Salesópolis, to Rasgão Dam, in Pirapora do Bom Jesus, covering 39 municipalities. Data from the planorbidic chart of the malacology laboratory of ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Raquel Gardini Sanches Palasio Thays de Jesus Rossignoli Riccardo Costabile Simone Di Sessa Fernanda Pires Ohlweiler Francisco Chiaravalloti-Neto Source Type: research

Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation
This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future stud...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Nilton Barnab é Rodrigues Lu ís Eduardo Martinez Villegas Ana Paula Marques Duarte Alessandra Silva Orfan ó Breno Dos Anjos Costa Rafael Nacif Pimenta Marcus V G Lacerda Wuelton M Monteiro N ágila Francinete Costa Secundino Paulo Filemon Paolucci Pime Source Type: research

Host DNA repair response to oxidative damage is modulated by Trypanosoma cruzi in a strain-dependent manner
Acta Trop. 2021 Sep 9:106127. doi: 10.1016/j.actatropica.2021.106127. Online ahead of print.ABSTRACTThe conservation of genomic integrity and stability is essential for cell survival. DNA Damage Responses (DDRs) are considered of paramount importance for all living beings and involve mechanisms of cell cycle regulation and damage-specific DNA repair pathways. Hydrogen peroxide (H2O2) is a compound that, in supraphysiological concentrations, damages biomolecules including the DNA, causing base modifications and strand breaks. There is evidence that Trypanosoma cruzi, the protozoan that causes Chagas disease, interferes in t...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Ester Rose Aline Moraes Tatiana Shiroma Nadjar Nitz Ana de C ássia Rosa Riccardo Pratesi Luciana Hagstr öm Juliana Lott de Carvalho Mariana Hecht Source Type: research

Current status of human liver fluke infections in the Greater Mekong Subregion
Acta Trop. 2021 Sep 9:106133. doi: 10.1016/j.actatropica.2021.106133. Online ahead of print.ABSTRACTThe Greater Mekong Subregion (GMS) is a trans-national region of the Mekong River basin in Southeast Asia comprising Cambodia, the People's Republic of China (specifically Yunnan Province and Guangxi Zhuang Autonomous Region), Lao People's Democratic Republic (Lao PDR), Myanmar, Thailand, and Vietnam home to more than 340 million people or almost 4.5% of the global population. Human liver fluke infections caused by O. viverrini and C. sinensis have been major public health problems in this region for decades. Opisthorchiasis...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Banchob Sripa Apiporn T Suwannatrai Somphou Sayasone Do Dung Trung Virak Khieu Yichao Yang Source Type: research

Role of two aminoacyl-tRNA synthetase associated proteins (Endothelial Monocyte Activating Polypeptides 1 and 2) of Leishmania donovani in chemotaxis of human monocytes
Acta Trop. 2021 Sep 9:106128. doi: 10.1016/j.actatropica.2021.106128. Online ahead of print.ABSTRACTVisceral leishmaniasis is caused by the protozoan parasite Leishmania donovani. It is a fatal form of leishmaniasis prevalent in Indian subcontinent. Since there are no human licensed vaccines available for leishmaniasis, chemotherapeutic drugs remain the only means for combating parasitic infections. We have earlier identified a total of 26 amino-acyl tRNA synthetases (aaRS) along with five stand-alone editing domains and two aaRS-associated proteins in Leishmania donovani. In addition to their canonical role of tRNA aminoa...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Smriti Tandon Rohini Muthuswami Rentala Madhubala Source Type: research

Oestrid myiasis at a cross-road
Acta Trop. 2021 Sep 9:106131. doi: 10.1016/j.actatropica.2021.106131. Online ahead of print.ABSTRACTThe oestrids are a genuinely unique group of Diptera that represent many species whose larvae parasitize mammals, feeding on live tissues of a wide range of hosts, from mice to elephants, causing obligatory myiases. Those have had greatest impact on the activities of humans and their use of animals (e.g., as food, recreation, in the conduct of wars). To a large extent their impact has been controlled through the use of therapeutic application of synthetic chemical treatments. While in some limited cases eradication has been ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Douglas D Colwell Domenico Otranto Source Type: research

Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China
This study determined the prevalence of T. gondii, and its genotypes, in pork. A total of 1,223 diaphragm muscle samples were collected from eight slaughterhouses and 79 markets and detected by PCR amplification of the ITS gene. All of the positive samples were used for genotype identification by PCR-RFLP with 11 genetic markers. The total positive rate of T. gondii in Chongqing pork was 8.7%, and differences in T. gondii infection rates were found between different districts (0%-23.3%), seasons (e.g., 4.3% from Spring, 7.3% from Summer, 11.4% from Autumn, 12.0% from Winter) and years (2.7%- 14.3%). Six samples were succes...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Yehua Zhang Jianhua Xie Rongsheng Mi Hongquan Ling Lu Luo Haiyan Jia Xiaoli Zhang Yan Huang Haiyan Gong Xiangan Han Zheng Zeng Zhaoguo Chen Source Type: research

Spatial analysis of areas at risk for schistosomiasis in the Alto Tiet ê Basin, São Paulo, Brazil
Acta Trop. 2021 Sep 9:106132. doi: 10.1016/j.actatropica.2021.106132. Online ahead of print.ABSTRACTThe present study identified locations with the presence of Biomphalaria and the areas at risk for Schistosoma mansoni, the etiological agent of schistosomiasis, in the Metropolitan Region of São Paulo (MRSP), located in the Alto Tietê Basin (ATB), São Paulo, Brazil. The study area comprises floodplains of the Tietê River from its source, in Salesópolis, to Rasgão Dam, in Pirapora do Bom Jesus, covering 39 municipalities. Data from the planorbidic chart of the malacology laboratory of ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Raquel Gardini Sanches Palasio Thays de Jesus Rossignoli Riccardo Costabile Simone Di Sessa Fernanda Pires Ohlweiler Francisco Chiaravalloti-Neto Source Type: research

Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation
This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future stud...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Nilton Barnab é Rodrigues Lu ís Eduardo Martinez Villegas Ana Paula Marques Duarte Alessandra Silva Orfan ó Breno Dos Anjos Costa Rafael Nacif Pimenta Marcus V G Lacerda Wuelton M Monteiro N ágila Francinete Costa Secundino Paulo Filemon Paolucci Pime Source Type: research

Host DNA repair response to oxidative damage is modulated by Trypanosoma cruzi in a strain-dependent manner
Acta Trop. 2021 Sep 9:106127. doi: 10.1016/j.actatropica.2021.106127. Online ahead of print.ABSTRACTThe conservation of genomic integrity and stability is essential for cell survival. DNA Damage Responses (DDRs) are considered of paramount importance for all living beings and involve mechanisms of cell cycle regulation and damage-specific DNA repair pathways. Hydrogen peroxide (H2O2) is a compound that, in supraphysiological concentrations, damages biomolecules including the DNA, causing base modifications and strand breaks. There is evidence that Trypanosoma cruzi, the protozoan that causes Chagas disease, interferes in t...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Ester Rose Aline Moraes Tatiana Shiroma Nadjar Nitz Ana de C ássia Rosa Riccardo Pratesi Luciana Hagstr öm Juliana Lott de Carvalho Mariana Hecht Source Type: research

Current status of human liver fluke infections in the Greater Mekong Subregion
Acta Trop. 2021 Sep 9:106133. doi: 10.1016/j.actatropica.2021.106133. Online ahead of print.ABSTRACTThe Greater Mekong Subregion (GMS) is a trans-national region of the Mekong River basin in Southeast Asia comprising Cambodia, the People's Republic of China (specifically Yunnan Province and Guangxi Zhuang Autonomous Region), Lao People's Democratic Republic (Lao PDR), Myanmar, Thailand, and Vietnam home to more than 340 million people or almost 4.5% of the global population. Human liver fluke infections caused by O. viverrini and C. sinensis have been major public health problems in this region for decades. Opisthorchiasis...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Banchob Sripa Apiporn T Suwannatrai Somphou Sayasone Do Dung Trung Virak Khieu Yichao Yang Source Type: research

Role of two aminoacyl-tRNA synthetase associated proteins (Endothelial Monocyte Activating Polypeptides 1 and 2) of Leishmania donovani in chemotaxis of human monocytes
Acta Trop. 2021 Sep 9:106128. doi: 10.1016/j.actatropica.2021.106128. Online ahead of print.ABSTRACTVisceral leishmaniasis is caused by the protozoan parasite Leishmania donovani. It is a fatal form of leishmaniasis prevalent in Indian subcontinent. Since there are no human licensed vaccines available for leishmaniasis, chemotherapeutic drugs remain the only means for combating parasitic infections. We have earlier identified a total of 26 amino-acyl tRNA synthetases (aaRS) along with five stand-alone editing domains and two aaRS-associated proteins in Leishmania donovani. In addition to their canonical role of tRNA aminoa...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Smriti Tandon Rohini Muthuswami Rentala Madhubala Source Type: research

Oestrid myiasis at a cross-road
Acta Trop. 2021 Sep 9:106131. doi: 10.1016/j.actatropica.2021.106131. Online ahead of print.ABSTRACTThe oestrids are a genuinely unique group of Diptera that represent many species whose larvae parasitize mammals, feeding on live tissues of a wide range of hosts, from mice to elephants, causing obligatory myiases. Those have had greatest impact on the activities of humans and their use of animals (e.g., as food, recreation, in the conduct of wars). To a large extent their impact has been controlled through the use of therapeutic application of synthetic chemical treatments. While in some limited cases eradication has been ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Douglas D Colwell Domenico Otranto Source Type: research

Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China
This study determined the prevalence of T. gondii, and its genotypes, in pork. A total of 1,223 diaphragm muscle samples were collected from eight slaughterhouses and 79 markets and detected by PCR amplification of the ITS gene. All of the positive samples were used for genotype identification by PCR-RFLP with 11 genetic markers. The total positive rate of T. gondii in Chongqing pork was 8.7%, and differences in T. gondii infection rates were found between different districts (0%-23.3%), seasons (e.g., 4.3% from Spring, 7.3% from Summer, 11.4% from Autumn, 12.0% from Winter) and years (2.7%- 14.3%). Six samples were succes...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Yehua Zhang Jianhua Xie Rongsheng Mi Hongquan Ling Lu Luo Haiyan Jia Xiaoli Zhang Yan Huang Haiyan Gong Xiangan Han Zheng Zeng Zhaoguo Chen Source Type: research

Spatial analysis of areas at risk for schistosomiasis in the Alto Tiet ê Basin, São Paulo, Brazil
Acta Trop. 2021 Sep 9:106132. doi: 10.1016/j.actatropica.2021.106132. Online ahead of print.ABSTRACTThe present study identified locations with the presence of Biomphalaria and the areas at risk for Schistosoma mansoni, the etiological agent of schistosomiasis, in the Metropolitan Region of São Paulo (MRSP), located in the Alto Tietê Basin (ATB), São Paulo, Brazil. The study area comprises floodplains of the Tietê River from its source, in Salesópolis, to Rasgão Dam, in Pirapora do Bom Jesus, covering 39 municipalities. Data from the planorbidic chart of the malacology laboratory of ...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Raquel Gardini Sanches Palasio Thays de Jesus Rossignoli Riccardo Costabile Simone Di Sessa Fernanda Pires Ohlweiler Francisco Chiaravalloti-Neto Source Type: research

Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation
This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future stud...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Nilton Barnab é Rodrigues Lu ís Eduardo Martinez Villegas Ana Paula Marques Duarte Alessandra Silva Orfan ó Breno Dos Anjos Costa Rafael Nacif Pimenta Marcus V G Lacerda Wuelton M Monteiro N ágila Francinete Costa Secundino Paulo Filemon Paolucci Pime Source Type: research

Host DNA repair response to oxidative damage is modulated by Trypanosoma cruzi in a strain-dependent manner
Acta Trop. 2021 Sep 9:106127. doi: 10.1016/j.actatropica.2021.106127. Online ahead of print.ABSTRACTThe conservation of genomic integrity and stability is essential for cell survival. DNA Damage Responses (DDRs) are considered of paramount importance for all living beings and involve mechanisms of cell cycle regulation and damage-specific DNA repair pathways. Hydrogen peroxide (H2O2) is a compound that, in supraphysiological concentrations, damages biomolecules including the DNA, causing base modifications and strand breaks. There is evidence that Trypanosoma cruzi, the protozoan that causes Chagas disease, interferes in t...
Source: Acta Tropica - September 12, 2021 Category: Infectious Diseases Authors: Ester Rose Aline Moraes Tatiana Shiroma Nadjar Nitz Ana de C ássia Rosa Riccardo Pratesi Luciana Hagstr öm Juliana Lott de Carvalho Mariana Hecht Source Type: research

Vaccination of mice with recombinant novel aminopeptidase P and cathepsin X alone or in combination induces protective immunity against Trichinella spiralis infection
The objective of this study was to investigate the protective immunity elicited by immunization with TsAPP and TsCX alone and TsAPP-TsCX in combination in a mouse model. The results demonstrate that subcutaneous vaccination of mice with rTsAPP, rTsCX or rTsAPP+ rTsCX elicited a systemic humoral response (high levels of serum IgG, IgG1/IgG2a and IgA) and significant local gut mucosal sIgA responses. The vaccination with rTsAPP, rTsCX or rTsAPP+ rTsCX also induced a systemic and local mixed Th1/Th2 response, as demonstrated by clear elevation levels of IFN-γ and IL-4 in vaccinated mice. Vaccination of mice with rTsAPP+...
Source: Acta Tropica - September 11, 2021 Category: Infectious Diseases Authors: Jie Zeng Xin Zhuo Zhang Ru Zhang Shu Wei Yan Yan Yan Song Shao Rong Long Ruo Dan Liu Zhong Quan Wang Jing Cui Source Type: research

Morphological and genetic revision of cytoform 'L' of the Simulium (Simulium) tani complex from Taiwan, and cytoform 'D' of the S. (S.) suzukii complex (Diptera: Simuliidae) from the Nansei Islands, Japan
Acta Trop. 2021 Sep 8:106124. doi: 10.1016/j.actatropica.2021.106124. Online ahead of print.ABSTRACTThe species status of two cytoforms of two species complexes in the Simulium (Simulium) tuberosum species-group, which are almost indistinguishable in the pupal stage, was morphologically and genetically evaluated. Cytoform 'L' of the S. (S.) tani Takaoka & Davies complex, previously recognized as S. (S.) suzukii Rubtsov in Taiwan, is described as a new species, S. (S.) jianshiense. It is morphologically distinguishable as adults from two members of the S. (S.) suzukii complex: cytoform 'C' from Hokkaido, selected to rep...
Source: Acta Tropica - September 11, 2021 Category: Infectious Diseases Authors: Hiroyuki Takaoka Yasushi Otsuka Van Lun Low Peter H Adler Masako Fukuda Yao-Te Huang Zubaidah Ya'cob Source Type: research

The effect of regulatory T cells in Schistosoma-mediated protection against type 2 diabetes
Acta Trop. 2021 Sep 3:106073. doi: 10.1016/j.actatropica.2021.106073. Online ahead of print.ABSTRACTIn western societies, the prevalence of type 2 diabetes (T2D) is related to the hygiene hypothesis, which implies that reduced exposure to infectious factors results in a loss of the immune stimulation necessary to form the immune system during development. In fact, it has been reported that parasites, such as Schistosoma, can improve or prevent the development of T2D, which may be related to the activity of immune cells, including regulatory T cells (Tregs). Hence, Schistosoma, Tregs, and T2D share a close relationship. Sch...
Source: Acta Tropica - September 6, 2021 Category: Infectious Diseases Authors: Yan-Ru Gao Xue-Zhi Sun Ru Li Chun-Lian Tang Rong-Hui Zhang Ya-Wen Zhu Xiu-Rong Li Qun Pan Source Type: research

The effect of regulatory T cells in Schistosoma-mediated protection against type 2 diabetes
Acta Trop. 2021 Sep 3:106073. doi: 10.1016/j.actatropica.2021.106073. Online ahead of print.ABSTRACTIn western societies, the prevalence of type 2 diabetes (T2D) is related to the hygiene hypothesis, which implies that reduced exposure to infectious factors results in a loss of the immune stimulation necessary to form the immune system during development. In fact, it has been reported that parasites, such as Schistosoma, can improve or prevent the development of T2D, which may be related to the activity of immune cells, including regulatory T cells (Tregs). Hence, Schistosoma, Tregs, and T2D share a close relationship. Sch...
Source: Acta Tropica - September 6, 2021 Category: Infectious Diseases Authors: Yan-Ru Gao Xue-Zhi Sun Ru Li Chun-Lian Tang Rong-Hui Zhang Ya-Wen Zhu Xiu-Rong Li Qun Pan Source Type: research