A new species of Trichodina lishuiensis n. sp. (Ciliophora: Trichodinidae) in urinary bladder of Odorrana schmackeri (Amphibia: Ranidae) from Zhejiang, China
In this study we describe a new species named Trichodina lishuiensis from the bladder of Odorrana schmackeri collected in Zhejiang, China, with the prevalence of 20% (9/45). We identified T. lishuiensis as a new species by morphological comparison and molecular analysis. The ciliates were observed using the dry-silver and protargol staining methods, as well as SEM (scanning electron microscopy). Trichodina lishuiensis is a small species (cell diameter 31.8-43.9 μm), with incompact denticles connection, medium-wide blades and thick rays. We also sequenced a 1712 bp-long fragment of the small subunit ribosomal RNA gene (S...
Source: Acta Tropica - June 19, 2021 Category: Infectious Diseases Authors: Weitian Zhou Runqiu Wang Weishan Zhao Hong Zou Wenxiang Li Shangong Wu Ming Li Guitang Wang Source Type: research

Change in susceptibility response of Aedes aegypti (Diptera: Culicidae) to organophosphate insecticide and Copaifera oleoresin
Acta Trop. 2021 Jun 16:106014. doi: 10.1016/j.actatropica.2021.106014. Online ahead of print.ABSTRACTThe growth of resistance in vector mosquitoes to insecticides, especially the organophosphate Temephos can facilitate the transmission of various disease agents worldwide. Consequently, it arises a challenge to public health agencies, which is the urgency use of other possibilities as botanical insecticides. Such insecticides have specific properties against insects due to the plant's ability to synthesize products derived from its secondary metabolism. The diversity and complexity of active compounds of botanical insectici...
Source: Acta Tropica - June 19, 2021 Category: Infectious Diseases Authors: Josiane Somariva Prophiro Mario Antonio Navarro da Silva Joice Guilherme de Oliveira Paula Fassicolo Variza Alessandra B de Lemos Harry Luiz Pilz-J únior Onilda Santos da Silva Source Type: research

Peptides of H. sapiens and P. falciparum that are predicted to bind strongly to HLA-A*24:02 and homologous to a SARS-CoV-2 peptide
CONCLUSION: Immune responses to the identified-peptides with similar sequences and strong binding affinities to HLA-A*24:02 can be related to autoimmune response risk in individuals with HLA-A*24:02 serotypes, upon getting infected with SARS-CoV-2 or P. falciparum.PMID:34146538 | DOI:10.1016/j.actatropica.2021.106013 (Source: Acta Tropica)
Source: Acta Tropica - June 19, 2021 Category: Infectious Diseases Authors: Yekbun Adiguzel Source Type: research

Exploring Rabies Endemicity in Pakistan: Major Constraints & amp; Possible Solutions
Acta Trop. 2021 Jun 15:106011. doi: 10.1016/j.actatropica.2021.106011. Online ahead of print.ABSTRACTRabies, the oldest recorded viral zoonosis in the Indo-Pakistan subcontinent, is a neglected and lingering endemic disease in Pakistan. The review of online available rabies-related journals, papers and reports through platforms and electronic databases have provided the basis for a detailed analysis of the rabies situation in Pakistan. Only published materials related to various research areas of rabies in Pakistan were included and meaningful conclusions were developed to identify major constraints and generate an intelle...
Source: Acta Tropica - June 18, 2021 Category: Infectious Diseases Authors: Waqas Ahmad Muhammad Ahsan Naeem Qaiser Akram Sarfraz Ahmad Muhammad Younus Source Type: research

Exploring Rabies Endemicity in Pakistan: Major Constraints & amp; Possible Solutions
Acta Trop. 2021 Jun 15:106011. doi: 10.1016/j.actatropica.2021.106011. Online ahead of print.ABSTRACTRabies, the oldest recorded viral zoonosis in the Indo-Pakistan subcontinent, is a neglected and lingering endemic disease in Pakistan. The review of online available rabies-related journals, papers and reports through platforms and electronic databases have provided the basis for a detailed analysis of the rabies situation in Pakistan. Only published materials related to various research areas of rabies in Pakistan were included and meaningful conclusions were developed to identify major constraints and generate an intelle...
Source: Acta Tropica - June 18, 2021 Category: Infectious Diseases Authors: Waqas Ahmad Muhammad Ahsan Naeem Qaiser Akram Sarfraz Ahmad Muhammad Younus Source Type: research

West Nile fever upsurge in a Greek regional unit, 2020
Acta Trop. 2021 Jun 12:106010. doi: 10.1016/j.actatropica.2021.106010. Online ahead of print.ABSTRACTDuring the 2020 West Nile virus (WNV) transmission season, Greece was the most affected EU Member State. More than one third of human cases occurred in Serres regional unit in northern Greece, which is characterized by the presence of a major wetland (Kerkini lake and Strimon river). A total of 2809 Culex pipiens mosquitoes collected in Serres were grouped into 70 pools and tested for WNV. Ten (14.3%) pools were found positive, and all WNV sequences belonged to the Central European subclade of WNV lineage 2. The first human...
Source: Acta Tropica - June 15, 2021 Category: Infectious Diseases Authors: Anna Papa Katerina Tsioka Sandra Gewehr Stella Kalaitzopouou Danai Pervanidou Anna Vakali Chrysovalantou Kefaloudi Styliani Pappa Xanthoula Louka Spiros Mourelatos Source Type: research

West Nile fever upsurge in a Greek regional unit, 2020
Acta Trop. 2021 Jun 12:106010. doi: 10.1016/j.actatropica.2021.106010. Online ahead of print.ABSTRACTDuring the 2020 West Nile virus (WNV) transmission season, Greece was the most affected EU Member State. More than one third of human cases occurred in Serres regional unit in northern Greece, which is characterized by the presence of a major wetland (Kerkini lake and Strimon river). A total of 2809 Culex pipiens mosquitoes collected in Serres were grouped into 70 pools and tested for WNV. Ten (14.3%) pools were found positive, and all WNV sequences belonged to the Central European subclade of WNV lineage 2. The first human...
Source: Acta Tropica - June 15, 2021 Category: Infectious Diseases Authors: Anna Papa Katerina Tsioka Sandra Gewehr Stella Kalaitzopouou Danai Pervanidou Anna Vakali Chrysovalantou Kefaloudi Styliani Pappa Xanthoula Louka Spiros Mourelatos Source Type: research

West Nile fever upsurge in a Greek regional unit, 2020
Acta Trop. 2021 Jun 12:106010. doi: 10.1016/j.actatropica.2021.106010. Online ahead of print.ABSTRACTDuring the 2020 West Nile virus (WNV) transmission season, Greece was the most affected EU Member State. More than one third of human cases occurred in Serres regional unit in northern Greece, which is characterized by the presence of a major wetland (Kerkini lake and Strimon river). A total of 2809 Culex pipiens mosquitoes collected in Serres were grouped into 70 pools and tested for WNV. Ten (14.3%) pools were found positive, and all WNV sequences belonged to the Central European subclade of WNV lineage 2. The first human...
Source: Acta Tropica - June 15, 2021 Category: Infectious Diseases Authors: Anna Papa Katerina Tsioka Sandra Gewehr Stella Kalaitzopouou Danai Pervanidou Anna Vakali Chrysovalantou Kefaloudi Styliani Pappa Xanthoula Louka Spiros Mourelatos Source Type: research

Relationship between vertical stratification and feeding habits of mosquito (Diptera: Culicidae) assemblages collected in conservation units in the green belt of the city of S ão Paulo, Brazil
Acta Trop. 2021 Jun 11:106009. doi: 10.1016/j.actatropica.2021.106009. Online ahead of print.ABSTRACTSão Paulo is one of the largest cities in the world and has several characteristics that favor a diversity of urban and wild mosquitoes. Little is known about how variations in mosquito diversity and feeding preferences for different hosts in different vegetation strata can influence the risk of pathogen transmission to humans. We investigated vertical stratification of mosquitoes and its relationship with vertebrate hosts in environments with different degrees of conservation in two conservation units in the city of...
Source: Acta Tropica - June 14, 2021 Category: Infectious Diseases Authors: Eduardo Evangelista Ant ônio Ralph Medeiros-Sousa Walter Ceretti-Junior Rafael Oliveira-Christe Ramon Wilk-da-Silva Ana Maria Ribeiro de Castro Duarte Daniel Pagotto Vendrami Gabriela Cristina de Carvalho Luis Filipe Mucci Mauro Toledo Marrelli Source Type: research

Seasonal distribution of Haemaphysalis longicornis (Acari: Ixodidae) and detection of SFTS virus in Gyeongbuk Province, Republic of Korea, 2018
Acta Trop. 2021 Jun 11:106012. doi: 10.1016/j.actatropica.2021.106012. Online ahead of print.ABSTRACTThe seasonal distribution of hard ticks was investigated in 2018 in Gyeongbuk Province, Republic of Korea. Ticks were assayed for severe fever with thrombocytopenia syndrome virus (SFTSV). Ticks were collected monthly using CO2-baited traps from April to November in four habitats (grasslands, grave sites, hiking trails, and mixed forests). Based on morphological and molecular identification, Haemaphysalis longicornis was the most commonly collected species, followed by H. flava and Ixodes nipponensis. Ticks were more common...
Source: Acta Tropica - June 14, 2021 Category: Infectious Diseases Authors: JaeSeok Lee KyungHwan Moon Myeonghwan Kim Wook-Gyo Lee Hee-Ii Lee Jong Kyun Park Young Ho Kim Source Type: research

Relationship between vertical stratification and feeding habits of mosquito (Diptera: Culicidae) assemblages collected in conservation units in the green belt of the city of S ão Paulo, Brazil
Acta Trop. 2021 Jun 11:106009. doi: 10.1016/j.actatropica.2021.106009. Online ahead of print.ABSTRACTSão Paulo is one of the largest cities in the world and has several characteristics that favor a diversity of urban and wild mosquitoes. Little is known about how variations in mosquito diversity and feeding preferences for different hosts in different vegetation strata can influence the risk of pathogen transmission to humans. We investigated vertical stratification of mosquitoes and its relationship with vertebrate hosts in environments with different degrees of conservation in two conservation units in the city of...
Source: Acta Tropica - June 14, 2021 Category: Infectious Diseases Authors: Eduardo Evangelista Ant ônio Ralph Medeiros-Sousa Walter Ceretti-Junior Rafael Oliveira-Christe Ramon Wilk-da-Silva Ana Maria Ribeiro de Castro Duarte Daniel Pagotto Vendrami Gabriela Cristina de Carvalho Luis Filipe Mucci Mauro Toledo Marrelli Source Type: research

Seasonal distribution of Haemaphysalis longicornis (Acari: Ixodidae) and detection of SFTS virus in Gyeongbuk Province, Republic of Korea, 2018
Acta Trop. 2021 Jun 11:106012. doi: 10.1016/j.actatropica.2021.106012. Online ahead of print.ABSTRACTThe seasonal distribution of hard ticks was investigated in 2018 in Gyeongbuk Province, Republic of Korea. Ticks were assayed for severe fever with thrombocytopenia syndrome virus (SFTSV). Ticks were collected monthly using CO2-baited traps from April to November in four habitats (grasslands, grave sites, hiking trails, and mixed forests). Based on morphological and molecular identification, Haemaphysalis longicornis was the most commonly collected species, followed by H. flava and Ixodes nipponensis. Ticks were more common...
Source: Acta Tropica - June 14, 2021 Category: Infectious Diseases Authors: JaeSeok Lee KyungHwan Moon Myeonghwan Kim Wook-Gyo Lee Hee-Ii Lee Jong Kyun Park Young Ho Kim Source Type: research

Anopheles (Nyssorhynchus) tadei: A New Species of the Oswaldoi-konderi Complex (Diptera, Anophelinae) and its Morphological and Molecular Distinctions from An. konderi sensu stricto
Acta Trop. 2021 Jun 10:106004. doi: 10.1016/j.actatropica.2021.106004. Online ahead of print.ABSTRACTThe Oswaldoi-konderi Complex (Anopheles, Nyssorhynchus) is composed of five species that have been distinguished and delimited using DNA sequences of mitochondrial and nuclear genes. At least two species of the complex have been formally described, namely Anopheles oswaldoi s.s. and An. konderi; however, the identity of An. konderi s.s. is unclear because two morphologically similar species co-exist in the type-locality in the municipality of Coari, Amazonas state, Brazil. Moreover, the study of resurrection and designation...
Source: Acta Tropica - June 13, 2021 Category: Infectious Diseases Authors: Jos é Ferreira Saraiva Vera Margarete Scarpassa Source Type: research

Intra and peridomiciliary comparison of density, sex ratio and gonotrophic stage of Phlebotomus sergenti in an active anthroponotic cutaneous leishmaniasis focus in Morocco
The objective of this work was to compare the intra and extradomiciliary density, sex ratio and gonotrophic stage of sand flies from a recent active focus in Morocco. This field study is based on the need to optimize the effectiveness of control programs. Two different capture methods, CDC light traps and sticky traps, were used at two different times of the year, corresponding with the peaks of sand fly abundance. 7,815 sand flies were captured and classified into 13 species belonging to genera Sergentomyia (50.8%) and Phlebotomus (49.2%). Phlebotomus sergenti was the most abundant and frequent species of the genus Phlebo...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: Patricia Gij ón-Robles Naima Abattouy Victoriano Corpas-L ópez Nora El Khalfaoui Francisco Morillas-M árquez Myriam Riyad Joaquina Mart ín-Sánchez Victoriano D íaz-Sáez Source Type: research

Discovery and Validation of a Novel Target of Molluscicides against Oncomelania hupensis, the Intermediate Host of Schistosoma japonicum
In this study, 196 strains of actinomycetes isolated from marshland soil samples were tested for molluscicidal activity against Oncomelania hupensis. Five strains demonstrated molluscicidal activity, of which the molluscicidal efficiency of Actinomycetes strain A183 was the maximum. After the fermentation supernatant of actinomycetes A183 was extracted with ethyl acetate (EWEA), the LC50 of the EWEA after leaching for 48 h and 72 h were 0.2688 and 0.2195 mg/L, respectively. The effect of EWEA on the key points of energy metabolism was determined. We noted that 1 mg/L of EWEA (A813) significantly reduced the activity of mit...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: Yuntian Xing Suyang Zhang Guoli Qu Jianrong Dai Jiakai Yao Bainian Feng Source Type: research

Clinico-microbiological description and evaluation of rapid lateral flow immunoassay and PCR for detection of Burkholderia pseudomallei from patients hospitalized with sepsis and pneumonia: A twenty-one months study from Odisha, India
Acta Trop. 2021 Jun 9:105994. doi: 10.1016/j.actatropica.2021.105994. Online ahead of print.ABSTRACTEstablishing a diagnosis of melioidosis based on clinical grounds is difficult in hospitalized patients with sepsis or community acquired pneumonia (CAP). We aimed to ascertain the prevalence, clinico-epidemiological and laboratory profile of melioidosis in hospitalized patients with sepsis or CAP, and to evaluate the diagnostic utility of rapid lateral flow immunoassay (LFI) and PCR in comparison with culture. In all patients with sepsis or CAP, blood, sputum/throat swab, and urine sample were subjected to culture along wit...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: Dr Anjuna Radhakrishnan Dr Bijayini Behera Dr Baijayantimala Mishra Prasanta Raghab Mohapatra Dr Rajesh Kumar Dr Arvind Kumar Singh Source Type: research

Association between ocular toxoplasmosis and APEX1 and MYD88 polymorphism
Acta Trop. 2021 Jun 9:106006. doi: 10.1016/j.actatropica.2021.106006. Online ahead of print.ABSTRACTOcular toxoplasmosis (OT) is the most common form of posterior uveitis, and in some countries, it is the most frequent cause of visual impairment. Studies demonstrate that the polymorphism in genes involved with the immune response can be related both to the occurrence and to the recurrence of OT. Thus, the present study aimed to analyze the association between OT and the polymorphism of the APEX1 (rs1130409) and MyD88 (rs7744) genes. The studied sample consisted of 48 volunteers with OT and 96 asymptomatic volunteers, but p...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: D ébora de Almeida Aloise Wendel Coura-Vital Mari ângela Carneiro Marlus Ven âncio Rodrigues Gislani Ac ásia da Silva Toscano Ramiza Bernardino da Silva Rita de C ássia Barreto Silva-Portela Fabr ícia Lima Fontes-Dantas Lucymara Fassarella Agnez-Lim Source Type: research

Intra and peridomiciliary comparison of density, sex ratio and gonotrophic stage of Phlebotomus sergenti in an active anthroponotic cutaneous leishmaniasis focus in Morocco
The objective of this work was to compare the intra and extradomiciliary density, sex ratio and gonotrophic stage of sand flies from a recent active focus in Morocco. This field study is based on the need to optimize the effectiveness of control programs. Two different capture methods, CDC light traps and sticky traps, were used at two different times of the year, corresponding with the peaks of sand fly abundance. 7,815 sand flies were captured and classified into 13 species belonging to genera Sergentomyia (50.8%) and Phlebotomus (49.2%). Phlebotomus sergenti was the most abundant and frequent species of the genus Phlebo...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: Patricia Gij ón-Robles Naima Abattouy Victoriano Corpas-L ópez Nora El Khalfaoui Francisco Morillas-M árquez Myriam Riyad Joaquina Mart ín-Sánchez Victoriano D íaz-Sáez Source Type: research

Discovery and Validation of a Novel Target of Molluscicides against Oncomelania hupensis, the Intermediate Host of Schistosoma japonicum
In this study, 196 strains of actinomycetes isolated from marshland soil samples were tested for molluscicidal activity against Oncomelania hupensis. Five strains demonstrated molluscicidal activity, of which the molluscicidal efficiency of Actinomycetes strain A183 was the maximum. After the fermentation supernatant of actinomycetes A183 was extracted with ethyl acetate (EWEA), the LC50 of the EWEA after leaching for 48 h and 72 h were 0.2688 and 0.2195 mg/L, respectively. The effect of EWEA on the key points of energy metabolism was determined. We noted that 1 mg/L of EWEA (A813) significantly reduced the activity of mit...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: Yuntian Xing Suyang Zhang Guoli Qu Jianrong Dai Jiakai Yao Bainian Feng Source Type: research

Clinico-microbiological description and evaluation of rapid lateral flow immunoassay and PCR for detection of Burkholderia pseudomallei from patients hospitalized with sepsis and pneumonia: A twenty-one months study from Odisha, India
Acta Trop. 2021 Jun 9:105994. doi: 10.1016/j.actatropica.2021.105994. Online ahead of print.ABSTRACTEstablishing a diagnosis of melioidosis based on clinical grounds is difficult in hospitalized patients with sepsis or community acquired pneumonia (CAP). We aimed to ascertain the prevalence, clinico-epidemiological and laboratory profile of melioidosis in hospitalized patients with sepsis or CAP, and to evaluate the diagnostic utility of rapid lateral flow immunoassay (LFI) and PCR in comparison with culture. In all patients with sepsis or CAP, blood, sputum/throat swab, and urine sample were subjected to culture along wit...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: Dr Anjuna Radhakrishnan Dr Bijayini Behera Dr Baijayantimala Mishra Prasanta Raghab Mohapatra Dr Rajesh Kumar Dr Arvind Kumar Singh Source Type: research

Association between ocular toxoplasmosis and APEX1 and MYD88 polymorphism
Acta Trop. 2021 Jun 9:106006. doi: 10.1016/j.actatropica.2021.106006. Online ahead of print.ABSTRACTOcular toxoplasmosis (OT) is the most common form of posterior uveitis, and in some countries, it is the most frequent cause of visual impairment. Studies demonstrate that the polymorphism in genes involved with the immune response can be related both to the occurrence and to the recurrence of OT. Thus, the present study aimed to analyze the association between OT and the polymorphism of the APEX1 (rs1130409) and MyD88 (rs7744) genes. The studied sample consisted of 48 volunteers with OT and 96 asymptomatic volunteers, but p...
Source: Acta Tropica - June 12, 2021 Category: Infectious Diseases Authors: D ébora de Almeida Aloise Wendel Coura-Vital Mari ângela Carneiro Marlus Ven âncio Rodrigues Gislani Ac ásia da Silva Toscano Ramiza Bernardino da Silva Rita de C ássia Barreto Silva-Portela Fabr ícia Lima Fontes-Dantas Lucymara Fassarella Agnez-Lim Source Type: research

A systematic review of Trichomonas vaginalis in Turkey from 2002 to 2020
Acta Trop. 2021 Jun 4:105995. doi: 10.1016/j.actatropica.2021.105995. Online ahead of print.ABSTRACTTrichomonas vaginalis is a flagellate human parasite responsible for the most common non-viral sexually transmitted infection (STI) in the world. We aim to estimate the prevalence and basic characteristics of T. vaginalis infection in the Turkish population from 2002 to 2020. Six international and two national electronic databases were searched using keywords of "Trichomonas vaginalis" and "Turkey" both in English and Turkish languages. Searching motors for the present study included the following: Google...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Yunus Emre Beyhan Source Type: research

Theileria equi and Babesia caballi in horses in the Czech Republic
Acta Trop. 2021 Jun 4:105993. doi: 10.1016/j.actatropica.2021.105993. Online ahead of print.ABSTRACTTheileria equi and Babesia caballi are protozoan agents causing equine piroplasmosis, endemic in countries all over the world. The aim of this study was to detect antibodies to T. equi and B. caballi in horses in the Czech Republic and to investigate the origin of the infection. Blood sera from 711 horses were examined with competitive enzyme-linked immunosorbent assay; positive samples were verified with indirect fluorescence immunoassay. Antibodies to T. equi and B. caballi were detected in eight (1.1%) and three (0.4%) ho...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Tereza B ělková Eva B ártová Dagmar Řičařová Petr Jahn Vendula Jandov á David Modr ý Krist ýna Hrazdilová Kamil Sedl ák Source Type: research

A systematic review of Trichomonas vaginalis in Turkey from 2002 to 2020
Acta Trop. 2021 Jun 4:105995. doi: 10.1016/j.actatropica.2021.105995. Online ahead of print.ABSTRACTTrichomonas vaginalis is a flagellate human parasite responsible for the most common non-viral sexually transmitted infection (STI) in the world. We aim to estimate the prevalence and basic characteristics of T. vaginalis infection in the Turkish population from 2002 to 2020. Six international and two national electronic databases were searched using keywords of "Trichomonas vaginalis" and "Turkey" both in English and Turkish languages. Searching motors for the present study included the following: Google...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Yunus Emre Beyhan Source Type: research

Theileria equi and Babesia caballi in horses in the Czech Republic
Acta Trop. 2021 Jun 4:105993. doi: 10.1016/j.actatropica.2021.105993. Online ahead of print.ABSTRACTTheileria equi and Babesia caballi are protozoan agents causing equine piroplasmosis, endemic in countries all over the world. The aim of this study was to detect antibodies to T. equi and B. caballi in horses in the Czech Republic and to investigate the origin of the infection. Blood sera from 711 horses were examined with competitive enzyme-linked immunosorbent assay; positive samples were verified with indirect fluorescence immunoassay. Antibodies to T. equi and B. caballi were detected in eight (1.1%) and three (0.4%) ho...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Tereza B ělková Eva B ártová Dagmar Řičařová Petr Jahn Vendula Jandov á David Modr ý Krist ýna Hrazdilová Kamil Sedl ák Source Type: research

A systematic review of Trichomonas vaginalis in Turkey from 2002 to 2020
Acta Trop. 2021 Jun 4:105995. doi: 10.1016/j.actatropica.2021.105995. Online ahead of print.ABSTRACTTrichomonas vaginalis is a flagellate human parasite responsible for the most common non-viral sexually transmitted infection (STI) in the world. We aim to estimate the prevalence and basic characteristics of T. vaginalis infection in the Turkish population from 2002 to 2020. Six international and two national electronic databases were searched using keywords of "Trichomonas vaginalis" and "Turkey" both in English and Turkish languages. Searching motors for the present study included the following: Google...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Yunus Emre Beyhan Source Type: research

Theileria equi and Babesia caballi in horses in the Czech Republic
Acta Trop. 2021 Jun 4:105993. doi: 10.1016/j.actatropica.2021.105993. Online ahead of print.ABSTRACTTheileria equi and Babesia caballi are protozoan agents causing equine piroplasmosis, endemic in countries all over the world. The aim of this study was to detect antibodies to T. equi and B. caballi in horses in the Czech Republic and to investigate the origin of the infection. Blood sera from 711 horses were examined with competitive enzyme-linked immunosorbent assay; positive samples were verified with indirect fluorescence immunoassay. Antibodies to T. equi and B. caballi were detected in eight (1.1%) and three (0.4%) ho...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Tereza B ělková Eva B ártová Dagmar Řičařová Petr Jahn Vendula Jandov á David Modr ý Krist ýna Hrazdilová Kamil Sedl ák Source Type: research

A systematic review of Trichomonas vaginalis in Turkey from 2002 to 2020
Acta Trop. 2021 Jun 4:105995. doi: 10.1016/j.actatropica.2021.105995. Online ahead of print.ABSTRACTTrichomonas vaginalis is a flagellate human parasite responsible for the most common non-viral sexually transmitted infection (STI) in the world. We aim to estimate the prevalence and basic characteristics of T. vaginalis infection in the Turkish population from 2002 to 2020. Six international and two national electronic databases were searched using keywords of "Trichomonas vaginalis" and "Turkey" both in English and Turkish languages. Searching motors for the present study included the following: Google...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Yunus Emre Beyhan Source Type: research

Theileria equi and Babesia caballi in horses in the Czech Republic
Acta Trop. 2021 Jun 4:105993. doi: 10.1016/j.actatropica.2021.105993. Online ahead of print.ABSTRACTTheileria equi and Babesia caballi are protozoan agents causing equine piroplasmosis, endemic in countries all over the world. The aim of this study was to detect antibodies to T. equi and B. caballi in horses in the Czech Republic and to investigate the origin of the infection. Blood sera from 711 horses were examined with competitive enzyme-linked immunosorbent assay; positive samples were verified with indirect fluorescence immunoassay. Antibodies to T. equi and B. caballi were detected in eight (1.1%) and three (0.4%) ho...
Source: Acta Tropica - June 7, 2021 Category: Infectious Diseases Authors: Tereza B ělková Eva B ártová Dagmar Řičařová Petr Jahn Vendula Jandov á David Modr ý Krist ýna Hrazdilová Kamil Sedl ák Source Type: research

Serological reactivity against T. cruzi-derived antigens: evaluation of their suitability for the assessment of response to treatment in chronic Chagas disease
Acta Trop. 2021 Jun 3:105990. doi: 10.1016/j.actatropica.2021.105990. Online ahead of print.ABSTRACTChagas disease, caused by the protozoan Trypanosoma cruzi, affects more than 6 million people worldwide. Following a mostly asymptomatic acute phase, the disease progresses to a long-lasting chronic phase throughout which life-threatening disorders to the heart and/or gastrointestinal tract will manifest in about 30% of those chronically infected. During the chronic phase, the parasitemia is low and intermittent, while a high level of anti-T. cruzi antibodies persist for years. These two features hamper post-chemotherapeutic...
Source: Acta Tropica - June 6, 2021 Category: Infectious Diseases Authors: Julio Alonso-Padilla Manuel Carlos L ópez M ónica Esteva Maan Zrein Aina Casellas Inmaculada G ómez Elodie Granjon Susana M éndez Celia Benitez Andres Mariano Ruiz Sergi Sanz Joaquim Gasc ón M Carmen Thomas Maria-Jesus Pinazo NHEPACHA Study Group Source Type: research

Serological reactivity against T. cruzi-derived antigens: evaluation of their suitability for the assessment of response to treatment in chronic Chagas disease
Acta Trop. 2021 Jun 3:105990. doi: 10.1016/j.actatropica.2021.105990. Online ahead of print.ABSTRACTChagas disease, caused by the protozoan Trypanosoma cruzi, affects more than 6 million people worldwide. Following a mostly asymptomatic acute phase, the disease progresses to a long-lasting chronic phase throughout which life-threatening disorders to the heart and/or gastrointestinal tract will manifest in about 30% of those chronically infected. During the chronic phase, the parasitemia is low and intermittent, while a high level of anti-T. cruzi antibodies persist for years. These two features hamper post-chemotherapeutic...
Source: Acta Tropica - June 6, 2021 Category: Infectious Diseases Authors: Julio Alonso-Padilla Manuel Carlos L ópez M ónica Esteva Maan Zrein Aina Casellas Inmaculada G ómez Elodie Granjon Susana M éndez Celia Benitez Andres Mariano Ruiz Sergi Sanz Joaquim Gasc ón M Carmen Thomas Maria-Jesus Pinazo NHEPACHA Study Group Source Type: research

The Delayed Death-Causing Nature of Rosmarinus officinalis Leaf Extracts and Their Mixture within Experimental Chronic Toxoplasmosis: Therapeutic and Prophylactic Implications
CONCLUSIONS: R. officinalis displays a therapeutic rather than prophylactic potential, indicating the emergence of an effective safe alternative therapy.PMID:34089696 | DOI:10.1016/j.actatropica.2021.105992 (Source: Acta Tropica)
Source: Acta Tropica - June 5, 2021 Category: Infectious Diseases Authors: Enas F Abdel Hamed Nahed E Mostafa Eman M Fawzy Mohamed N Ibrahim Rasha A Attia Marwa A Salama Source Type: research

Expression and distribution of glutathione transferases in protoscoleces of Echinococcus granulosus sensu lato
Acta Trop. 2021 Jun 2:105991. doi: 10.1016/j.actatropica.2021.105991. Online ahead of print.ABSTRACTGlutathione transferases (GSTs) belong to a diverse superfamily of multifunctional proteins involved in metabolic detoxification. In helminth parasite, GSTs are particularly relevant since they are also involved in host immunomodulation. Echinococcus granulosus sensu lato (s.l.) is a cestode parasite known to express at least three phylogenetically distant cytosolic GSTs: EgGST1 and EgGST2 previously grouped within Mu and Sigma classes, respectively; and EgGST3 related to both Omega and Sigma classes. To better characterize ...
Source: Acta Tropica - June 5, 2021 Category: Infectious Diseases Authors: Paula Arbildi Silvana La-Rocca Alejandra Kun Karina R Lorenzato Karina M Monteiro Arnaldo Zaha Gustavo Mourglia-Ettlin Henrique B Ferreira Ver ónica Fernández Source Type: research

The Delayed Death-Causing Nature of Rosmarinus officinalis Leaf Extracts and Their Mixture within Experimental Chronic Toxoplasmosis: Therapeutic and Prophylactic Implications
CONCLUSIONS: R. officinalis displays a therapeutic rather than prophylactic potential, indicating the emergence of an effective safe alternative therapy.PMID:34089696 | DOI:10.1016/j.actatropica.2021.105992 (Source: Acta Tropica)
Source: Acta Tropica - June 5, 2021 Category: Infectious Diseases Authors: Enas F Abdel Hamed Nahed E Mostafa Eman M Fawzy Mohamed N Ibrahim Rasha A Attia Marwa A Salama Source Type: research

Expression and distribution of glutathione transferases in protoscoleces of Echinococcus granulosus sensu lato
Acta Trop. 2021 Jun 2:105991. doi: 10.1016/j.actatropica.2021.105991. Online ahead of print.ABSTRACTGlutathione transferases (GSTs) belong to a diverse superfamily of multifunctional proteins involved in metabolic detoxification. In helminth parasite, GSTs are particularly relevant since they are also involved in host immunomodulation. Echinococcus granulosus sensu lato (s.l.) is a cestode parasite known to express at least three phylogenetically distant cytosolic GSTs: EgGST1 and EgGST2 previously grouped within Mu and Sigma classes, respectively; and EgGST3 related to both Omega and Sigma classes. To better characterize ...
Source: Acta Tropica - June 5, 2021 Category: Infectious Diseases Authors: Paula Arbildi Silvana La-Rocca Alejandra Kun Karina R Lorenzato Karina M Monteiro Arnaldo Zaha Gustavo Mourglia-Ettlin Henrique B Ferreira Ver ónica Fernández Source Type: research

Challenges to improved animal rabies surveillance: Experiences from pilot implementation of decentralized diagnostic units in Chad
Acta Trop. 2021 May 28:105984. doi: 10.1016/j.actatropica.2021.105984. Online ahead of print.ABSTRACTBetter surveillance is desperately needed to guide rabies prevention and control to achieve the goal of zero dog-mediated human rabies by 2030, defined by the World Health Organization (WHO) and partners in 2015. With the help of funding from the Vaccine Alliance (GAVI) learning agenda, we implemented animal rabies surveillance based on One Health communication, improved accessibility of diagnostic testing and facilitated sample transport to increase case detection in three regions of Chad. Through the project, rabies surve...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Kemdongarti Na ïssengar Assandi Oussiguere Enos Madaye Nodjimbadem Mbaipago Rolande Mindekem Ronelngar Moyengar Alladoumngar Madjadinan Richard Ngandolo Jakob Zinsstag Monique L échenne Source Type: research

Low genetic variation in Echinococcus multilocularis from the Western Sichuan Plateau of China revealed by microsatellite and mitochondrial DNA markers
In conclusion, both the microsatellite and mtDNA markers showed low variability within the Tibetan population of E. multilocularis. An EmsB profile and a cox1 haplotype were found to be predominant in the study area, which appears to remain steady for over a decade. The results reinforce the higher potential of the microsatellite DNA marker with high discriminative power to identify the very low genetic polymorphism of E. multilocularis than that of the partial cox1 sequencing. The data obtained in the study would be helpful to enlarge the data pool to further probe the possible origins and dispersal of E. multilocularis i...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Jing-Ye Shang Guang-Jia Zhang Sha Liao Wen-Jie Yu Wei He Qi Wang Yan Huang Qian Wang Yan-Xi Long Yang Liu Bo Zhong Source Type: research

An appraisal of the scientific current situation and new perspectives in the treatment of cutaneous leishmaniasis
Acta Trop. 2021 May 28:105988. doi: 10.1016/j.actatropica.2021.105988. Online ahead of print.ABSTRACTLeishmaniasis is a Neglected Tropical Diseases caused by protozoan parasites of the genus Leishmania. It is a major health problem in many tropical and subtropical regions of the world and can produce three different clinical manifestations, among which cutaneous leishmaniasis has a higher incidence in the world than the other clinical forms. There are no recognized and reliable means of chemoprophylaxis or vaccination against infections with different forms of leishmaniasis. In addition, chemotherapy, unfortunately, remain...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Cintia Alejandra Briones Nieva Alicia Graciela Cid Anal ía Irma Romero Mar ía Fernanda García-Bustos Mercedes Villegas Jos é María Bermúdez Source Type: research

A new antigen detection ELISA for the diagnosis of Strongyloides infection
Acta Trop. 2021 May 28:105986. doi: 10.1016/j.actatropica.2021.105986. Online ahead of print.ABSTRACTSerodiagnosis is an essential component of the laboratory diagnosis of Strongyloides infection and is usually performed using an indirect IgG antibody test. A direct antigen detection method can complement the IgG assay, particularly for detecting early infection and post-treatment follow-up. In the present study, a recombinant scFv monoclonal antibody against NIE recombinant protein (rMAb23) that we had previously produced was used to develop a Strongyloides antigen detection ELISA (SsAg-ELISA). The assay is based on detec...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Dinesh Balachandra Anizah Rahumatullah Theam Soon Lim Fatin Hamimi Mustafa Hussain Ahmad Nor Suhada Anuar Rahmah Noordin Source Type: research

Histological, histochemical and energy disorders caused by R-limonene on Aedes aegypti L. larvae (Diptera: Culicidae)
Acta Trop. 2021 May 28:105987. doi: 10.1016/j.actatropica.2021.105987. Online ahead of print.ABSTRACTThe study evaluated the effects of R-limonene, in sublethal concentration, on the histology, histochemistry, biochemistry, and carbohydrates and proteins levels in the third instar Aedes aegypti larvae. The R-limonene (LC50 of 27 ppm) and control groups were analyzed 12 and 24h after the beginning of treatments. The midgut of the control larvae was composed of cylindrical and elongated cells with a spherical and central nucleus and regenerative cells with a pyramidal shape. After 12 hours of treatment, columnar cells, protu...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Fernanda M Oliveira Valeria Wanderley-Teixeira Glaucilane S Cruz Cristiane T S Silva Kamilla A Dutra Hilton N Costa Valeska A A Braga Edson J Silva Carolina A Guedes Thiago J S Alves Álvaro A C Teixeira Source Type: research

Challenges to improved animal rabies surveillance: Experiences from pilot implementation of decentralized diagnostic units in Chad
Acta Trop. 2021 May 28:105984. doi: 10.1016/j.actatropica.2021.105984. Online ahead of print.ABSTRACTBetter surveillance is desperately needed to guide rabies prevention and control to achieve the goal of zero dog-mediated human rabies by 2030, defined by the World Health Organization (WHO) and partners in 2015. With the help of funding from the Vaccine Alliance (GAVI) learning agenda, we implemented animal rabies surveillance based on One Health communication, improved accessibility of diagnostic testing and facilitated sample transport to increase case detection in three regions of Chad. Through the project, rabies surve...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Kemdongarti Na ïssengar Assandi Oussiguere Enos Madaye Nodjimbadem Mbaipago Rolande Mindekem Ronelngar Moyengar Alladoumngar Madjadinan Richard Ngandolo Jakob Zinsstag Monique L échenne Source Type: research

Low genetic variation in Echinococcus multilocularis from the Western Sichuan Plateau of China revealed by microsatellite and mitochondrial DNA markers
In conclusion, both the microsatellite and mtDNA markers showed low variability within the Tibetan population of E. multilocularis. An EmsB profile and a cox1 haplotype were found to be predominant in the study area, which appears to remain steady for over a decade. The results reinforce the higher potential of the microsatellite DNA marker with high discriminative power to identify the very low genetic polymorphism of E. multilocularis than that of the partial cox1 sequencing. The data obtained in the study would be helpful to enlarge the data pool to further probe the possible origins and dispersal of E. multilocularis i...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Jing-Ye Shang Guang-Jia Zhang Sha Liao Wen-Jie Yu Wei He Qi Wang Yan Huang Qian Wang Yan-Xi Long Yang Liu Bo Zhong Source Type: research

An appraisal of the scientific current situation and new perspectives in the treatment of cutaneous leishmaniasis
Acta Trop. 2021 May 28:105988. doi: 10.1016/j.actatropica.2021.105988. Online ahead of print.ABSTRACTLeishmaniasis is a Neglected Tropical Diseases caused by protozoan parasites of the genus Leishmania. It is a major health problem in many tropical and subtropical regions of the world and can produce three different clinical manifestations, among which cutaneous leishmaniasis has a higher incidence in the world than the other clinical forms. There are no recognized and reliable means of chemoprophylaxis or vaccination against infections with different forms of leishmaniasis. In addition, chemotherapy, unfortunately, remain...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Cintia Alejandra Briones Nieva Alicia Graciela Cid Anal ía Irma Romero Mar ía Fernanda García-Bustos Mercedes Villegas Jos é María Bermúdez Source Type: research

A new antigen detection ELISA for the diagnosis of Strongyloides infection
Acta Trop. 2021 May 28:105986. doi: 10.1016/j.actatropica.2021.105986. Online ahead of print.ABSTRACTSerodiagnosis is an essential component of the laboratory diagnosis of Strongyloides infection and is usually performed using an indirect IgG antibody test. A direct antigen detection method can complement the IgG assay, particularly for detecting early infection and post-treatment follow-up. In the present study, a recombinant scFv monoclonal antibody against NIE recombinant protein (rMAb23) that we had previously produced was used to develop a Strongyloides antigen detection ELISA (SsAg-ELISA). The assay is based on detec...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Dinesh Balachandra Anizah Rahumatullah Theam Soon Lim Fatin Hamimi Mustafa Hussain Ahmad Nor Suhada Anuar Rahmah Noordin Source Type: research

Histological, histochemical and energy disorders caused by R-limonene on Aedes aegypti L. larvae (Diptera: Culicidae)
Acta Trop. 2021 May 28:105987. doi: 10.1016/j.actatropica.2021.105987. Online ahead of print.ABSTRACTThe study evaluated the effects of R-limonene, in sublethal concentration, on the histology, histochemistry, biochemistry, and carbohydrates and proteins levels in the third instar Aedes aegypti larvae. The R-limonene (LC50 of 27 ppm) and control groups were analyzed 12 and 24h after the beginning of treatments. The midgut of the control larvae was composed of cylindrical and elongated cells with a spherical and central nucleus and regenerative cells with a pyramidal shape. After 12 hours of treatment, columnar cells, protu...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Fernanda M Oliveira Valeria Wanderley-Teixeira Glaucilane S Cruz Cristiane T S Silva Kamilla A Dutra Hilton N Costa Valeska A A Braga Edson J Silva Carolina A Guedes Thiago J S Alves Álvaro A C Teixeira Source Type: research

Challenges to improved animal rabies surveillance: Experiences from pilot implementation of decentralized diagnostic units in Chad
Acta Trop. 2021 May 28:105984. doi: 10.1016/j.actatropica.2021.105984. Online ahead of print.ABSTRACTBetter surveillance is desperately needed to guide rabies prevention and control to achieve the goal of zero dog-mediated human rabies by 2030, defined by the World Health Organization (WHO) and partners in 2015. With the help of funding from the Vaccine Alliance (GAVI) learning agenda, we implemented animal rabies surveillance based on One Health communication, improved accessibility of diagnostic testing and facilitated sample transport to increase case detection in three regions of Chad. Through the project, rabies surve...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Kemdongarti Na ïssengar Assandi Oussiguere Enos Madaye Nodjimbadem Mbaipago Rolande Mindekem Ronelngar Moyengar Alladoumngar Madjadinan Richard Ngandolo Jakob Zinsstag Monique L échenne Source Type: research

Low genetic variation in Echinococcus multilocularis from the Western Sichuan Plateau of China revealed by microsatellite and mitochondrial DNA markers
In conclusion, both the microsatellite and mtDNA markers showed low variability within the Tibetan population of E. multilocularis. An EmsB profile and a cox1 haplotype were found to be predominant in the study area, which appears to remain steady for over a decade. The results reinforce the higher potential of the microsatellite DNA marker with high discriminative power to identify the very low genetic polymorphism of E. multilocularis than that of the partial cox1 sequencing. The data obtained in the study would be helpful to enlarge the data pool to further probe the possible origins and dispersal of E. multilocularis i...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Jing-Ye Shang Guang-Jia Zhang Sha Liao Wen-Jie Yu Wei He Qi Wang Yan Huang Qian Wang Yan-Xi Long Yang Liu Bo Zhong Source Type: research

An appraisal of the scientific current situation and new perspectives in the treatment of cutaneous leishmaniasis
Acta Trop. 2021 May 28:105988. doi: 10.1016/j.actatropica.2021.105988. Online ahead of print.ABSTRACTLeishmaniasis is a Neglected Tropical Diseases caused by protozoan parasites of the genus Leishmania. It is a major health problem in many tropical and subtropical regions of the world and can produce three different clinical manifestations, among which cutaneous leishmaniasis has a higher incidence in the world than the other clinical forms. There are no recognized and reliable means of chemoprophylaxis or vaccination against infections with different forms of leishmaniasis. In addition, chemotherapy, unfortunately, remain...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Cintia Alejandra Briones Nieva Alicia Graciela Cid Anal ía Irma Romero Mar ía Fernanda García-Bustos Mercedes Villegas Jos é María Bermúdez Source Type: research

A new antigen detection ELISA for the diagnosis of Strongyloides infection
Acta Trop. 2021 May 28:105986. doi: 10.1016/j.actatropica.2021.105986. Online ahead of print.ABSTRACTSerodiagnosis is an essential component of the laboratory diagnosis of Strongyloides infection and is usually performed using an indirect IgG antibody test. A direct antigen detection method can complement the IgG assay, particularly for detecting early infection and post-treatment follow-up. In the present study, a recombinant scFv monoclonal antibody against NIE recombinant protein (rMAb23) that we had previously produced was used to develop a Strongyloides antigen detection ELISA (SsAg-ELISA). The assay is based on detec...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Dinesh Balachandra Anizah Rahumatullah Theam Soon Lim Fatin Hamimi Mustafa Hussain Ahmad Nor Suhada Anuar Rahmah Noordin Source Type: research

Histological, histochemical and energy disorders caused by R-limonene on Aedes aegypti L. larvae (Diptera: Culicidae)
Acta Trop. 2021 May 28:105987. doi: 10.1016/j.actatropica.2021.105987. Online ahead of print.ABSTRACTThe study evaluated the effects of R-limonene, in sublethal concentration, on the histology, histochemistry, biochemistry, and carbohydrates and proteins levels in the third instar Aedes aegypti larvae. The R-limonene (LC50 of 27 ppm) and control groups were analyzed 12 and 24h after the beginning of treatments. The midgut of the control larvae was composed of cylindrical and elongated cells with a spherical and central nucleus and regenerative cells with a pyramidal shape. After 12 hours of treatment, columnar cells, protu...
Source: Acta Tropica - May 31, 2021 Category: Infectious Diseases Authors: Fernanda M Oliveira Valeria Wanderley-Teixeira Glaucilane S Cruz Cristiane T S Silva Kamilla A Dutra Hilton N Costa Valeska A A Braga Edson J Silva Carolina A Guedes Thiago J S Alves Álvaro A C Teixeira Source Type: research