Blastocystis genetic diversity in animal and human samples from different departments of Colombia using complete sequencing of the 18S rRNA gene (SSU rRNA) by Oxford Nanopore Technologies (ONT)
Acta Trop. 2023 Dec 2;249:107090. doi: 10.1016/j.actatropica.2023.107090. Online ahead of print.ABSTRACTBlastocystis is an intestinal microeukaryote that has raised attention due to its wide distribution in animals and humans. The risk of zoonotic circulation primarily arises from close contact with infected animals. Therefore, the following study aimed to evaluate the diversity and frequency of Blastocystis subtypes in Colombian human and animal samples using complete sequencing of the 18S rRNA gene. For this purpose, 341 human stool samples and 277 animal fecal samples (from cattle, sheep, goat, pigs, cats, and dogs), we...
Source: Acta Tropica - December 4, 2023 Category: Infectious Diseases Authors: Paula Jim énez Marina Mu ñoz Lissa Cruz-Saavedra Anny Camargo Juan David Ram írez Source Type: research
Blastocystis genetic diversity in animal and human samples from different departments of Colombia using complete sequencing of the 18S rRNA gene (SSU rRNA) by Oxford Nanopore Technologies (ONT)
Acta Trop. 2023 Dec 2;249:107090. doi: 10.1016/j.actatropica.2023.107090. Online ahead of print.ABSTRACTBlastocystis is an intestinal microeukaryote that has raised attention due to its wide distribution in animals and humans. The risk of zoonotic circulation primarily arises from close contact with infected animals. Therefore, the following study aimed to evaluate the diversity and frequency of Blastocystis subtypes in Colombian human and animal samples using complete sequencing of the 18S rRNA gene. For this purpose, 341 human stool samples and 277 animal fecal samples (from cattle, sheep, goat, pigs, cats, and dogs), we...
Source: Acta Tropica - December 4, 2023 Category: Infectious Diseases Authors: Paula Jim énez Marina Mu ñoz Lissa Cruz-Saavedra Anny Camargo Juan David Ram írez Source Type: research
Blastocystis genetic diversity in animal and human samples from different departments of Colombia using complete sequencing of the 18S rRNA gene (SSU rRNA) by Oxford Nanopore Technologies (ONT)
Acta Trop. 2023 Dec 2:107090. doi: 10.1016/j.actatropica.2023.107090. Online ahead of print.ABSTRACTBlastocystis is an intestinal microeukaryote that has raised attention due to its wide distribution in animals and humans. The risk of zoonotic circulation primarily arises from close contact with infected animals. Therefore, the following study aimed to evaluate the diversity and frequency of Blastocystis subtypes in Colombian human and animal samples using complete sequencing of the 18S rRNA gene. For this purpose, 341 human stool samples and 277 animal fecal samples (from cattle, sheep, goat, pigs, cats, and dogs), were c...
Source: Acta Tropica - December 4, 2023 Category: Infectious Diseases Authors: Paula Jim énez Marina Mu ñoz Lissa Cruz-Saavedra Anny Camargo Juan David Ram írez Source Type: research
Integrating artificial intelligence and wing geometric morphometry to automate mosquito classification
Acta Trop. 2023 Dec 1:107089. doi: 10.1016/j.actatropica.2023.107089. Online ahead of print.ABSTRACTMosquitoes (Diptera: Culicidae) comprise over 3,500 global species, primarily in tropical regions, where the females act as disease vectors. Thus, identifying medically significant species is vital. In this context, Wing Geometric Morphometry (WGM) emerges as a precise and accessible method, excelling in species differentiation through mathematical approaches. Computational technologies and Artificial Intelligence (AI) promise to overcome WGM challenges, supporting mosquito identification. AI explores computers' thinking cap...
Source: Acta Tropica - December 3, 2023 Category: Infectious Diseases Authors: Vinicio Rodrigues de Lima Mauro C ésar Cafundó de Morais Karin Kirchgatter Source Type: research
Emergence of genotype III St. Louis encephalitis virus in the western United States potentially linked to a wetland in Argentina
In this study, genotype III SLEV was detected in mosquitoes collected in Mar Chiquita Lagoon (Córdoba, Argentina), an overwintering site for numerous migratory bird species. The genotype III SLEV sequence detected in this site shares the closest known ancestor with those introduced in Arizona in 2015. Our results highlight the potential significance of wetlands as key sites for arbovirus maintenance and emergence.PMID:38043673 | DOI:10.1016/j.actatropica.2023.107088 (Source: Acta Tropica)
Source: Acta Tropica - December 3, 2023 Category: Infectious Diseases Authors: Mauricio Beranek Carolina Torres Magdalena Laurito Adri án Farías Marta Contigiani Walter Almir ón Adri án Diaz Source Type: research
Integrating artificial intelligence and wing geometric morphometry to automate mosquito classification
Acta Trop. 2023 Dec 1:107089. doi: 10.1016/j.actatropica.2023.107089. Online ahead of print.ABSTRACTMosquitoes (Diptera: Culicidae) comprise over 3,500 global species, primarily in tropical regions, where the females act as disease vectors. Thus, identifying medically significant species is vital. In this context, Wing Geometric Morphometry (WGM) emerges as a precise and accessible method, excelling in species differentiation through mathematical approaches. Computational technologies and Artificial Intelligence (AI) promise to overcome WGM challenges, supporting mosquito identification. AI explores computers' thinking cap...
Source: Acta Tropica - December 3, 2023 Category: Infectious Diseases Authors: Vinicio Rodrigues de Lima Mauro C ésar Cafundó de Morais Karin Kirchgatter Source Type: research
Emergence of genotype III St. Louis encephalitis virus in the western United States potentially linked to a wetland in Argentina
In this study, genotype III SLEV was detected in mosquitoes collected in Mar Chiquita Lagoon (Córdoba, Argentina), an overwintering site for numerous migratory bird species. The genotype III SLEV sequence detected in this site shares the closest known ancestor with those introduced in Arizona in 2015. Our results highlight the potential significance of wetlands as key sites for arbovirus maintenance and emergence.PMID:38043673 | DOI:10.1016/j.actatropica.2023.107088 (Source: Acta Tropica)
Source: Acta Tropica - December 3, 2023 Category: Infectious Diseases Authors: Mauricio Beranek Carolina Torres Magdalena Laurito Adri án Farías Marta Contigiani Walter Almir ón Adri án Diaz Source Type: research
Utility of nucleic acid amplification test in the detection of tuberculosis in biological fluids from suspected TB patients in a cardiovascular center in the Philippines
CONCLUSION: We conclude that the TB-NAAT is a quick and consistent diagnostic test for TB detection. However, due to a comparably lower sensitivity than other previous studies, the utility of TB-NAAT alone may not be sufficient in the screening of TB patients. Likewise, the TB-NAAT cannot detect non-tuberculous mycobacteria, for which additional analysis may be needed.PMID:38036022 | DOI:10.1016/j.actatropica.2023.107078 (Source: Acta Tropica)
Source: Acta Tropica - November 30, 2023 Category: Infectious Diseases Authors: Othaniel Philip R Balisan John Ray T Galamay Laarnie N Cale-Subia Arlene M DE Luna Source Type: research
Morphological, molecular, and MALDI-TOF MS identification of bed bugs and associated Wolbachia species from Cameroon
This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus (Hemiptera: Cimicidae), in Cameroon. A total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in Yaoundé and Douala, Cameroon. The ability of MALDI-TOF MS to identify bed bugs was tested using heads for adults and cephalothoraxes for immature stages. Microorganism screening was performed by qPCR and confirmed by regular PCR and sequencing. Based on morphometrical criteria, four stages of immature bed bugs are represented. Of the 248 bed bug specimens morphologically identifie...
Source: Acta Tropica - November 30, 2023 Category: Infectious Diseases Authors: Yannick Ngnindji-Youdje Michel Lontsi-Demano Adama Zan Diarra Ahmat Mahamat Makaila Timol éon Tchuinkam Jean-Michel Berenger Philippe Parola Source Type: research
Utility of nucleic acid amplification test in the detection of tuberculosis in biological fluids from suspected TB patients in a cardiovascular center in the Philippines
CONCLUSION: We conclude that the TB-NAAT is a quick and consistent diagnostic test for TB detection. However, due to a comparably lower sensitivity than other previous studies, the utility of TB-NAAT alone may not be sufficient in the screening of TB patients. Likewise, the TB-NAAT cannot detect non-tuberculous mycobacteria, for which additional analysis may be needed.PMID:38036022 | DOI:10.1016/j.actatropica.2023.107078 (Source: Acta Tropica)
Source: Acta Tropica - November 30, 2023 Category: Infectious Diseases Authors: Othaniel Philip R Balisan John Ray T Galamay Laarnie N Cale-Subia Arlene M DE Luna Source Type: research
Morphological, molecular, and MALDI-TOF MS identification of bed bugs and associated Wolbachia species from Cameroon
This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus (Hemiptera: Cimicidae), in Cameroon. A total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in Yaoundé and Douala, Cameroon. The ability of MALDI-TOF MS to identify bed bugs was tested using heads for adults and cephalothoraxes for immature stages. Microorganism screening was performed by qPCR and confirmed by regular PCR and sequencing. Based on morphometrical criteria, four stages of immature bed bugs are represented. Of the 248 bed bug specimens morphologically identifie...
Source: Acta Tropica - November 30, 2023 Category: Infectious Diseases Authors: Yannick Ngnindji-Youdje Michel Lontsi-Demano Adama Zan Diarra Ahmat Mahamat Makaila Timol éon Tchuinkam Jean-Michel Berenger Philippe Parola Source Type: research
Utility of nucleic acid amplification test in the detection of tuberculosis in biological fluids from suspected TB patients in a cardiovascular center in the Philippines
CONCLUSION: We conclude that the TB-NAAT is a quick and consistent diagnostic test for TB detection. However, due to a comparably lower sensitivity than other previous studies, the utility of TB-NAAT alone may not be sufficient in the screening of TB patients. Likewise, the TB-NAAT cannot detect non-tuberculous mycobacteria, for which additional analysis may be needed.PMID:38036022 | DOI:10.1016/j.actatropica.2023.107078 (Source: Acta Tropica)
Source: Acta Tropica - November 30, 2023 Category: Infectious Diseases Authors: Othaniel Philip R Balisan John Ray T Galamay Laarnie N Cale-Subia Arlene M DE Luna Source Type: research
Morphological, molecular, and MALDI-TOF MS identification of bed bugs and associated Wolbachia species from Cameroon
This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus (Hemiptera: Cimicidae), in Cameroon. A total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in Yaoundé and Douala, Cameroon. The ability of MALDI-TOF MS to identify bed bugs was tested using heads for adults and cephalothoraxes for immature stages. Microorganism screening was performed by qPCR and confirmed by regular PCR and sequencing. Based on morphometrical criteria, four stages of immature bed bugs are represented. Of the 248 bed bug specimens morphologically identifie...
Source: Acta Tropica - November 30, 2023 Category: Infectious Diseases Authors: Yannick Ngnindji-Youdje Michel Lontsi-Demano Adama Zan Diarra Ahmat Mahamat Makaila Timol éon Tchuinkam Jean-Michel Berenger Philippe Parola Source Type: research
A compartmental model for Schistosoma japonicum transmission dynamics in the Philippines
Acta Trop. 2023 Nov 27:107084. doi: 10.1016/j.actatropica.2023.107084. Online ahead of print.ABSTRACTSchistosomiasis is a chronic and debilitating neglected tropical disease (NTD), second only to malaria as one of the most devastating parasitic diseases. Caused by a parasitic flatworm of the genus Schistosoma, infection occurs when skin comes in contact with contaminated freshwater that contains schistosome-hosting snails. The disease continues to be endemic in many regions of the Philippines, where it poses a significant public health challenge due to a lack of healthcare resources. In the Philippines, additional mammalia...
Source: Acta Tropica - November 29, 2023 Category: Infectious Diseases Authors: Yuan-Jen Kuo Gian Paras Taiyo Tagami Claire Yi Leslie J Camacho Aquino Hyunju Oh Jan Rycht ář Dewey Taylor Source Type: research
Corrigendum to: A novel sensitive hexaplex high-resolution melt assay for identification of five human Plasmodium species plus internal control (Acta Tropica, volume 248, article number 107020)
Acta Trop. 2023 Nov 28:107077. doi: 10.1016/j.actatropica.2023.107077. Online ahead of print.NO ABSTRACTPMID:38030507 | DOI:10.1016/j.actatropica.2023.107077 (Source: Acta Tropica)
Source: Acta Tropica - November 29, 2023 Category: Infectious Diseases Authors: Suttipat Srisutham Paweesuda Rattanakoch Kaewkanha Kijprasong Rungniran Sugaram Nantanat Kantaratanakul Theerarak Srinulgray Arjen M Dondorp Mallika Imwong Source Type: research
