In vitro drug susceptibility using a parasite-rescue and transformation assay of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis amastigotes against antileishmanial drugs
This study investigated the in vitro drug sensitivity of L. martiniquensis and L. orientalis, along with two reference species causing VL, namely L. donovani and L. infantum, against six antileishmanial drugs. Using a parasite-rescue and transformation assay, the results demonstrated that the IC50 values of amphotericin B (AmB), miltefosine (MIL), and sodium stibogluconate (Sb(III)) against all Leishmania species tested were within the sensitive range of each drug. On the contrary, the IC50 values of artemisinin (ART) and dihydroartemisinin (DHA), drugs primarily used for malaria treatment, were outside the sensitive range...
Source: Acta Tropica - November 22, 2023 Category: Infectious Diseases Authors: Sirinan Kimcharoensuk Saovanee Leelayoova Mathirut Mungthin Namfon Nawattanapaibool Peerapan Tan-Ariya Suradej Siripattanapipong Source Type: research
In vitro drug susceptibility using a parasite-rescue and transformation assay of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis amastigotes against antileishmanial drugs
This study investigated the in vitro drug sensitivity of L. martiniquensis and L. orientalis, along with two reference species causing VL, namely L. donovani and L. infantum, against six antileishmanial drugs. Using a parasite-rescue and transformation assay, the results demonstrated that the IC50 values of amphotericin B (AmB), miltefosine (MIL), and sodium stibogluconate (Sb(III)) against all Leishmania species tested were within the sensitive range of each drug. On the contrary, the IC50 values of artemisinin (ART) and dihydroartemisinin (DHA), drugs primarily used for malaria treatment, were outside the sensitive range...
Source: Acta Tropica - November 22, 2023 Category: Infectious Diseases Authors: Sirinan Kimcharoensuk Saovanee Leelayoova Mathirut Mungthin Namfon Nawattanapaibool Peerapan Tan-Ariya Suradej Siripattanapipong Source Type: research
In vitro drug susceptibility using a parasite-rescue and transformation assay of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis amastigotes against antileishmanial drugs
This study investigated the in vitro drug sensitivity of L. martiniquensis and L. orientalis, along with two reference species causing VL, namely L. donovani and L. infantum, against six antileishmanial drugs. Using a parasite-rescue and transformation assay, the results demonstrated that the IC50 values of amphotericin B (AmB), miltefosine (MIL), and sodium stibogluconate (Sb(III)) against all Leishmania species tested were within the sensitive range of each drug. On the contrary, the IC50 values of artemisinin (ART) and dihydroartemisinin (DHA), drugs primarily used for malaria treatment, were outside the sensitive range...
Source: Acta Tropica - November 22, 2023 Category: Infectious Diseases Authors: Sirinan Kimcharoensuk Saovanee Leelayoova Mathirut Mungthin Namfon Nawattanapaibool Peerapan Tan-Ariya Suradej Siripattanapipong Source Type: research
In vitro drug susceptibility using a parasite-rescue and transformation assay of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis amastigotes against antileishmanial drugs
This study investigated the in vitro drug sensitivity of L. martiniquensis and L. orientalis, along with two reference species causing VL, namely L. donovani and L. infantum, against six antileishmanial drugs. Using a parasite-rescue and transformation assay, the results demonstrated that the IC50 values of amphotericin B (AmB), miltefosine (MIL), and sodium stibogluconate (Sb(III)) against all Leishmania species tested were within the sensitive range of each drug. On the contrary, the IC50 values of artemisinin (ART) and dihydroartemisinin (DHA), drugs primarily used for malaria treatment, were outside the sensitive range...
Source: Acta Tropica - November 22, 2023 Category: Infectious Diseases Authors: Sirinan Kimcharoensuk Saovanee Leelayoova Mathirut Mungthin Namfon Nawattanapaibool Peerapan Tan-Ariya Suradej Siripattanapipong Source Type: research
Bioefficacy of the Ethanolic Crude Extract of the Wild Leek, Allium ampeloprasum L. (Amaryllidaceae), Against the Third and Fourth Larval Stages of Aedes aegypti L. (Culicidae)
In this study, the larvicidal activity of the crude extract of Allium ampeloprasum L. (Asparagales: Amaryllidaceae) was studied in concentrations ranging from 1 to 10,000 mg/L against the third (L3) and fourth (L4) larval instars of Aedes aegypti L. Larval mortality at 48 h were subjected to probit analysis and Kruskal-Wallis H Test to estimate lethal concentrations and to determine significant means among the groups, respectively. Results show that the crude extract of A. ampeloprasum L. demonstrated larvicidal activity against the L3 and L4 Ae. aegypti L. Concentrations corresponding to 50% mortality (Lethal Concentratio...
Source: Acta Tropica - November 20, 2023 Category: Infectious Diseases Authors: Michael Villase ñor Yu Ashia Barodi Abdulcarim Mark Isaiah Nicolasora Baligod Francheska Sophia Edquiban Dela Cruz Charlene Joyce Villaflor Ordo ñez Arlene Garcia-Bertuso Source Type: research
Bioefficacy of the Ethanolic Crude Extract of the Wild Leek, Allium ampeloprasum L. (Amaryllidaceae), Against the Third and Fourth Larval Stages of Aedes aegypti L. (Culicidae)
In this study, the larvicidal activity of the crude extract of Allium ampeloprasum L. (Asparagales: Amaryllidaceae) was studied in concentrations ranging from 1 to 10,000 mg/L against the third (L3) and fourth (L4) larval instars of Aedes aegypti L. Larval mortality at 48 h were subjected to probit analysis and Kruskal-Wallis H Test to estimate lethal concentrations and to determine significant means among the groups, respectively. Results show that the crude extract of A. ampeloprasum L. demonstrated larvicidal activity against the L3 and L4 Ae. aegypti L. Concentrations corresponding to 50% mortality (Lethal Concentratio...
Source: Acta Tropica - November 20, 2023 Category: Infectious Diseases Authors: Michael Villase ñor Yu Ashia Barodi Abdulcarim Mark Isaiah Nicolasora Baligod Francheska Sophia Edquiban Dela Cruz Charlene Joyce Villaflor Ordo ñez Arlene Garcia-Bertuso Source Type: research
Characterization of a novel dipeptidyl peptidase 1 of Trichinella spiralis and its participation in larval invasion
Acta Trop. 2023 Nov 15;249:107076. doi: 10.1016/j.actatropica.2023.107076. Online ahead of print.ABSTRACTThe research aimed to describe a new Trichinella spiralis dipeptidyl peptidase 1 (TsDPP1) and investigate its functions in the larval invasion of intestinal epithelial cells (IECs). The gene TsDPP1 was successfully replicated and produced in Escherichia coli BL21 (DE3), showing a strong immune response. TsDPP1 was detected in diverse stages of T. spiralis and showed significant expression in the intestine infective larvae (IIL) and adult worms at 6 days post infection, as confirmed by qPCR and Western blot analysis. The...
Source: Acta Tropica - November 17, 2023 Category: Infectious Diseases Authors: Shu Wei Yan Yong Kang Cheng Qi Qi Lu Ru Zhang Ruo Dan Liu Shao Rong Long Zhong Quan Wang Jing Cui Source Type: research
Characterization of a novel dipeptidyl peptidase 1 of Trichinella spiralis and its participation in larval invasion
Acta Trop. 2023 Nov 15:107076. doi: 10.1016/j.actatropica.2023.107076. Online ahead of print.ABSTRACTThe research aimed to describe a new Trichinella spiralis dipeptidyl peptidase 1 (TsDPP1) and investigate its functions in the larval invasion of intestinal epithelial cells (IECs). The gene TsDPP1 was successfully replicated and produced in Escherichia coli BL21 (DE3), showing a strong immune response. TsDPP1 was detected in diverse stages of T. spiralis and showed significant expression in the intestine infective larvae (IIL) and adult worms at 6 days post infection, as confirmed by qPCR and Western blot analysis. The pri...
Source: Acta Tropica - November 17, 2023 Category: Infectious Diseases Authors: Shu Wei Yan Yong Kang Cheng Qi Qi Lu Ru Zhang Ruo Dan Liu Shao Rong Long Zhong Quan Wang Jing Cui Source Type: research
Characterization of a novel dipeptidyl peptidase 1 of Trichinella spiralis and its participation in larval invasion
Acta Trop. 2023 Nov 15:107076. doi: 10.1016/j.actatropica.2023.107076. Online ahead of print.ABSTRACTThe research aimed to describe a new Trichinella spiralis dipeptidyl peptidase 1 (TsDPP1) and investigate its functions in the larval invasion of intestinal epithelial cells (IECs). The gene TsDPP1 was successfully replicated and produced in Escherichia coli BL21 (DE3), showing a strong immune response. TsDPP1 was detected in diverse stages of T. spiralis and showed significant expression in the intestine infective larvae (IIL) and adult worms at 6 days post infection, as confirmed by qPCR and Western blot analysis. The pri...
Source: Acta Tropica - November 17, 2023 Category: Infectious Diseases Authors: Shu Wei Yan Yong Kang Cheng Qi Qi Lu Ru Zhang Ruo Dan Liu Shao Rong Long Zhong Quan Wang Jing Cui Source Type: research
Pediculosis capitis risk factors in schoolchildren: hair thickness and hair length
The objective of the study was to analyze the pediculosis risk using geographical location, gender, age and individual hair characteristics as variables. Pediculosis was diagnosed through the detection of living lice in the hair. This cross-sectional school-based epidemiological study was conducted in 310 schoolchildren aged 1 to 13 years of schools in 4 municipalities situated in the State of Paraná, Brazil. The prevalence of head louse infection in primary school students was 49.35%. The Odds Ratio of presence of pediculosis (OR) was estimated using multivariate logistic regression analysis. The results obtained indicat...
Source: Acta Tropica - November 15, 2023 Category: Infectious Diseases Authors: M A Valero J Haidamak T C de Oliveira Santos I Cristine Pr üss A Bisson C Santosdo Ros ário C Fantozzi M Morales-Su árez-Varela D R Klisiowicz Source Type: research
Pediculosis capitis risk factors in schoolchildren: hair thickness and hair length
The objective of the study was to analyze the pediculosis risk using geographical location, gender, age and individual hair characteristics as variables. Pediculosis was diagnosed through the detection of living lice in the hair. This cross-sectional school-based epidemiological study was conducted in 310 schoolchildren aged 1 to 13 years of schools in 4 municipalities situated in the State of Paraná, Brazil. The prevalence of head louse infection in primary school students was 49.35%. The Odds Ratio of presence of pediculosis (OR) was estimated using multivariate logistic regression analysis. The results obtained indicat...
Source: Acta Tropica - November 15, 2023 Category: Infectious Diseases Authors: M A Valero J Haidamak T C de Oliveira Santos I Cristine Pr üss A Bisson C Santosdo Ros ário C Fantozzi M Morales-Su árez-Varela D R Klisiowicz Source Type: research
Pediculosis capitis risk factors in schoolchildren: hair thickness and hair length
The objective of the study was to analyze the pediculosis risk using geographical location, gender, age and individual hair characteristics as variables. Pediculosis was diagnosed through the detection of living lice in the hair. This cross-sectional school-based epidemiological study was conducted in 310 schoolchildren aged 1 to 13 years of schools in 4 municipalities situated in the State of Paraná, Brazil. The prevalence of head louse infection in primary school students was 49.35%. The Odds Ratio of presence of pediculosis (OR) was estimated using multivariate logistic regression analysis. The results obtained indicat...
Source: Acta Tropica - November 15, 2023 Category: Infectious Diseases Authors: M A Valero J Haidamak T C de Oliveira Santos I Cristine Pr üss A Bisson C Santosdo Ros ário C Fantozzi M Morales-Su árez-Varela D R Klisiowicz Source Type: research
High risk of spoliation by bats in an indigenous village in Brazil: Warning of the re-emergence of rabies among the Maxakali People
Acta Trop. 2023 Nov 11:107073. doi: 10.1016/j.actatropica.2023.107073. Online ahead of print.ABSTRACTBat-mediated human rabies is a viral zoonotic disease that poses a serious threat to the public health of traditional peoples, especially indigenous populations that maintain primitive cultural and social habits, such as the Maxakali ethnic group, located in the southeastern region of Brazil. The sociocultural habit of this population led to the emergence between April and May 2022 of the viral spillover of rabies transmitted by bats, which decimated the lives of four children from this population who maintained contact wit...
Source: Acta Tropica - November 13, 2023 Category: Infectious Diseases Authors: Dilceu Silveira Tolentino J únior Source Type: research
Natural SARS-CoV-2 infection in dogs: Determination of viral loads, distributions, localizations, and pathology
This study considerably expanded on the knowledge of the pathology associated with natural SARS-CoV-2 infection in dogs, a scenario that is relatively infrequent but occasionally leads to fatal outcome. Furthermore, these findings suggest the potential utility of dogs as a model for studying SARS-CoV-2 infection in humans, warranting further investigation.PMID:37956819 | DOI:10.1016/j.actatropica.2023.107070 (Source: Acta Tropica)
Source: Acta Tropica - November 13, 2023 Category: Infectious Diseases Authors: Chutchai Piewbang Panida Poonsin Pattiya Lohavicharn Panitnan Punyathi Sawang Kesdangsakonwut Tanit Kasantikul Somporn Techanagmsuwan Source Type: research
Species and seasonality can affect recent trends in beak and feather disease virus prevalence in captive psittacine birds
In conclusion, our analysis of the trends in BFDV prevalence offers valuable insights into the prevalence or distribution of BFDV in the studied population. By monitoring BFDV prevalence, identifying high-risk species, and understanding seasonal patterns, we can develop targeted management approaches to control the spread of the virus. This information is crucial for mitigating the impact of BFDV on aviculture.PMID:37956820 | DOI:10.1016/j.actatropica.2023.107071 (Source: Acta Tropica)
Source: Acta Tropica - November 13, 2023 Category: Infectious Diseases Authors: A Saechin P Suksai L Sariya C Mongkolphan S Tangsudjai Source Type: research
