Molecules, Vol. 24, Pages 4516: A Machine Learning-Based Raman Spectroscopic Assay for the Identification of Burkholderia mallei and Related Species
inrich Neubauer
Burkholderia (B.) mallei, the causative agent of glanders, and B. pseudomallei, the causative agent of melioidosis in humans and animals, are genetically closely related. The high infectious potential of both organisms, their serological cross-reactivity, and similar clinical symptoms in human and animals make the differentiation from each other and other Burkholderia species challenging. The increased resistance against many antibiotics implies the need for fast and robust identification methods. The use of Raman microspectroscopy in microbial diagnostic has the potential for rapid and reliable identif...
Source: Molecules - December 9, 2019 Category: Chemistry Authors: Amira A. Moawad Anja Silge Thomas Bocklitz Katja Fischer Petra R ösch Uwe Roesler Mandy C. Elschner J ürgen Popp Heinrich Neubauer Tags: Article Source Type: research
Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera [Host Response and Inflammation]
Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators. The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities. Here, we descr...
Source: Infection and Immunity - November 17, 2019 Category: Infectious Diseases Authors: Sengyee, S., Yoon, S. H., West, T. E., Ernst, R. K., Chantratita, N. Tags: Host Response and Inflammation Source Type: research
Untargeted accurate identification of highly pathogenic bacteria directly from blood culture flasks.
Abstract
To improve the preparedness against exposure to highly pathogenic bacteria and to anticipate the wide variety of bacteria that can cause bloodstream infections (BSIs), a safe, unbiased and highly accurate identification method was developed. Our liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method can identify highly pathogenic bacteria, their near-neighbors and bacteria that are common causes of BSIs directly from positive blood culture flasks. The developed Peptide-Based Microbe Detection Engine (http://proteome2pathogen.com) relies on a two-step workflow: a genus-level search ...
Source: International Journal of Medical Microbiology - November 10, 2019 Category: Microbiology Authors: Berendsen EM, Levin E, Braakman R, Prodan A, van Leeuwen HC, Paauw A Tags: Int J Med Microbiol Source Type: research
Emerging role of biologics for the treatment of melioidosis and glanders
. (Source: Expert Opinion on Biological Therapy)
Source: Expert Opinion on Biological Therapy - October 14, 2019 Category: Drugs & Pharmacology Authors: Daniel Tapia Javier I. Sanchez-Villamil Alfredo G. Torres Source Type: research
GC-072: A Novel Therapeutic Candidate for Oral Treatment of Melioidosis and Infections Caused by Select Biothreat Pathogens.
Abstract
Burkholderia pseudomallei (B. pseudomallei), the etiological agent of melioidosis, is a Gram-negative bacterium with additional concern as a biothreat pathogen. The mortality rate from B. pseudomallei varies depending on the type of infection and extent of available health care, but in the case of septicemia left untreated it can range from 50 - 90%. Current therapy for melioidosis is biphasic, consisting of parenteral acute-phase treatment for two weeks or longer, followed by oral eradication-phase treatment lasting several months. An effective oral therapeutic for outpatient treatment of acute-p...
Source: Antimicrobial Agents and Chemotherapy - September 22, 2019 Category: Microbiology Authors: Shearer JD, Saylor ML, Butler CM, Treston AM, Heine HS, Chirakul S, Schweizer HP, Louie A, Drusano GL, Zumbrun SD, Warfield KL Tags: Antimicrob Agents Chemother Source Type: research
Development of an immunoblotting assay for serodiagnosis of Burkholderia mallei infection: the whole-cell proteome-based paradigm.
Conclusion: The whole-cell proteome-based immunoblot proved reliable and straightforward in our study. The prepared antigen was adaptable for application in immunoblotting. We assumed this improved immunoblotting system provides appropriate sensitivity and also specificity expected in serodiagnosis of glanders in endemic areas and typically in less-developed countries.
PMID: 31523407 [PubMed] (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - September 18, 2019 Category: Microbiology Tags: Iran J Microbiol Source Type: research
An in situ high-throughput screen identifies inhibitors of intracellular Burkholderia pseudomallei with therapeutic efficacy [Microbiology]
Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm) are Tier-1 Select Agents that cause melioidosis and glanders, respectively. These are highly lethal human infections with limited therapeutic options. Intercellular spread is a hallmark of Burkholderia pathogenesis, and its prominent ties to virulence make it an attractive therapeutic target. We developed a... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - September 9, 2019 Category: Science Authors: Philip L. Bulterys, Isabelle J. Toesca, Michael H. Norris, Jeffrey P. Maloy, Sorel T. Fitz-Gibbon, Bryan France, Babak Toffig, Marco Morselli, Nawarat Somprasong, Matteo Pellegrini, Herbert P. Schweizer, Apichai Tuanyok, Robert Damoiseaux, Christopher T. Tags: Biological Sciences Source Type: research
Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms
Burkholderia mallei (Bm) is a facultative intracellular bacterial pathogen causing highly-fatal glanders in solipeds and humans. The ability of Bm to thrive intracellularly is thought to be related to exploitatio... (Source: BMC Medical Genomics)
Source: BMC Medical Genomics - September 6, 2019 Category: Genetics & Stem Cells Authors: Sophie A. Aschenbroich, Eric R. Lafontaine, Maria Cecilia Lopez, Henry V. Baker and Robert J. Hogan Tags: Research article Source Type: research
A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans [Microbiology]
The Burkholderia genus encompasses many Gram-negative bacteria living in the rhizosphere. Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins. Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets. Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS–based analyses of O-glycans, and NMR-based st...
Source: Journal of Biological Chemistry - September 5, 2019 Category: Chemistry Authors: Yasmine Fathy Mohamed, Nichollas E. Scott, Antonio Molinaro, Carole Creuzenet, Ximena Ortega, Ganjana Lertmemongkolchai, Michael M. Tunney, Heather Green, Andrew M. Jones, David DeShazer, Bart J. Currie, Leonard J. Foster, Rebecca Ingram, Cristina De Cast Tags: Glycobiology and Extracellular Matrices Source Type: research
Melioidosis Patient Serum-Reactive Synthetic Tetrasaccharides Bearing the Predominant Epitopes of Burkholderia pseudomallei and Burkholderia mallei O-Antigens
Org. Biomol. Chem., 2019, Accepted Manuscript DOI: 10.1039/C9OB01711A, PaperMaude Cloutier, Emmanilo Delar, Kevin Muru, Seynabou Ndong, Robert R. Hoyeck, Taniya Kaewarpai, Narisara Chantratita, Mary N. Burtnick, Paul J. Brett, Charles Gauthier Melioidosis and glanders, respectively caused by the Gram-negative bacteriaBurkholderia pseudomallei (Bp) andBurkholderia mallei(Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic and Biomolecular Chemistry)
Source: RSC - Organic and Biomolecular Chemistry - September 4, 2019 Category: Molecular Biology Authors: Maude Cloutier Source Type: research
Evaluation of < i > Burkholderia mallei < /i > Δ < i > tonB < /i > Δ < i > hcp1 < /i > (CLH001) as a live attenuated vaccine in murine models of glanders and melioidosis
Conclusions/SignificanceOur data indicate that CLH001 serves as an effective live attenuated vaccine to prevent glanders and melioidosis. The quantity and quality of antibody responses as well as improving cell-mediated immune responses following vaccination need to be further investigated prior to advancement to preclinical studies. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - July 14, 2019 Category: Tropical Medicine Authors: Nittaya Khakhum Source Type: research
Molecular Mechanisms of AhpC in Resistance to Oxidative Stress in Burkholderia thailandensis
Burkholderia thailandensis is a model organism for human pathogens Burkholderia mallei and Burkholderia pseudomallei. The study of B. thailandensis peroxiredoxin is helpful for understanding the survival, pathogenic infection, and antibiotic resistance of its homologous species. Alkyl hydroperoxide reductase subunit C (AhpC) is an important peroxiredoxin involved in oxidative damage defense. Here, we report that BthAhpC exhibits broad specificity for peroxide substrates, including inorganic and organic peroxides and peroxynitrite. AhpC catalyzes the reduction of oxidants using the N-terminal conserved Cys57 as a peroxidati...
Source: Frontiers in Microbiology - July 1, 2019 Category: Microbiology Source Type: research
The reemergence of glanders as a zoonotic and occupational infection in Iran and neighboring countries
Glanders is a zoonotic infection, and because of recent outbreaks among Equidae family, the possibility of its reemergence among human populations is a crisis. The causative agent is Burkholderia mallei, a Gram-negative, aerobic and highly contagious bacterium causing severe impacts with low infectious dose transmitted via direct contact to respiratory secretions, skin exudates of animals and fomite. Despite high mortality rate, no proper vaccination has been developed to hinder the infection spread. The disease is more prevalent in Australia and Southeast Asia, but has been eradicated in developed countries. Glanders’ c...
Source: Reviews in Medical Microbiology - July 1, 2019 Category: Microbiology Tags: BACTERIOLOGY Source Type: research
A novel selective medium for the isolation of Burkholderia mallei from equine specimens
Burkholderia mallei is a Gram-negative bacterium that causes glanders, a zoonotic disease, especially in equine populations (e.g. horses, donkeys, and mules). B. mallei usually grows slowly on most culture media,... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - May 7, 2019 Category: Veterinary Research Authors: Yuta Kinoshita, Ashley K. Cloutier, David A. Rozak, Md. S. R. Khan, Hidekazu Niwa, Eri Uchida-Fujii, Yoshinari Katayama and Apichai Tuanyok Tags: Research article Source Type: research
