Untargeted accurate identification of highly pathogenic bacteria directly from blood culture flasks.
Untargeted accurate identification of highly pathogenic bacteria directly from blood culture flasks. Int J Med Microbiol. 2019 Nov 11;:151376 Authors: Berendsen EM, Levin E, Braakman R, Prodan A, van Leeuwen HC, Paauw A Abstract To improve the preparedness against exposure to highly pathogenic bacteria and to anticipate the wide variety of bacteria that can cause bloodstream infections (BSIs), a safe, unbiased and highly accurate identification method was developed. Our liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method can identify highly pathogenic bacteria, their near-neighbors and bacteria that are common causes of BSIs directly from positive blood culture flasks. The developed Peptide-Based Microbe Detection Engine (http://proteome2pathogen.com) relies on a two-step workflow: a genus-level search followed by a species-level search. This strategy enables the rapid identification of microorganisms based on the analyzed proteome. This method was successfully used to identify strains of Bacillus anthracis, Brucella abortus, Brucella melitensis, Brucella suis, Burkholderia pseudomallei, Burkholderia mallei, Francisella tularensis, Yersinia pestis and closely related species from simulated blood culture flasks. This newly developed LC-MS/MS method is a safe and rapid method for accurately identifying bacteria directly from positive blood culture flasks. PMID: 31784214 [PubMed - as supplied by publisher]
Publication date: Available online 9 October 2020Source: Saudi Journal of Biological SciencesAuthor(s): Reem Y Al jindan
N. E. Stone et al.
Authors: Casanovas-Moreno-Torres I, Gutiérrez-Soto B, Modovan TD, Expósito-Ruiz M, Navarro-Marí JM, Gutiérrez-Fernández J Abstract The activity of azithromycin against enteritis-producing agents other than Campylobacter spp. was studied. The susceptibility to azithromycin, through gradient test, of 88 clinical isolates (51 Salmonella spp., 23 Aeromonas spp., 10 Shigella sonnei and 4 Yersinia enterocolitica) for one year was studied prospectively. The results were compared with the activity of ampicillin, trimethoprim-sulfamethoxazole and ciprofloxacin by microdilution. For azithro...
Melioidosis is an infection caused by Gram-negative bacillus Burkholderia pseudomallei commonly present in the water and soil (Limmathurotsakul et al., 2013; Benoit et al., 2015). Clinically melioidosis can mimic other diseases such as cancer and tuberculosis, which in our case was suspected sarcoma.
Publication date: Available online 5 October 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Ke Zhang, Aijun Li, Yan Wang, Jinjuan Zhang, Yujie Chen, Huijing Wang, Ruixuan Shi, Yuanhao Qiu
Future Microbiology,Volume 15, Issue 13, Page 1207-1215, September 2020.
In conclusion, the mkDNA-based qPCR assay that we developed in this study can be used not only for diagnosis of both cutaneous and visceral leishmaniasis with high sensitivity and specificity, but also for evaluating the severity and treatment efficacy of this disease, presenting a rapid and accurate tool for clinical surveillance, treatment monitoring and the end point determination of leishmaniasis.
Conclusion: This study shows that there is a knowledge gap about anthrax among the people in the affected communities. Key drivers for the anthrax outbreak such as poor cultural beliefs and practices and wildlife-livestock-human interactions were observed in all the three subcounties studied. All these findings could imply a high risk of outbreak of anthrax in Arua and Ugandan agricultural communities where the public health programs are less standardized and less effective. PMID: 33014075 [PubMed]
Authors: Ekiri AB, Kilonzo C, Bird BH, VanWormer E, Wolking DJ, Smith WA, Masanja H, Kazwala RR, Mazet JAK Abstract In endemic African areas, such as Tanzania, Brucella spp. cause human febrile illnesses, which often go unrecognized and misdiagnosed, resulting in delayed diagnosis, underdiagnosis, and underreporting. Although rapid and affordable point-of-care tests, such as the Rose Bengal test (RBT), are available, acceptance and adoption of these tests at the national level are hindered by a lack of local diagnostic performance data. To address this need, evidence on the diagnostic performance of RBT as a human ...
Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-plate formic acid, and ethanol/formic acid tube extraction methods, were evaluated for their ability to render highly pathogenic organisms nonviable and safe for handling in a biosafety level 2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight-laboratory study to examine the u...