Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):162-167. doi: 10.1089/mab.2021.0023.ABSTRACTTo clarify the binding region of monoclonal antibodies (mAbs) to target molecules, it is very essential to understand the pharmacological function of each mAb. Although deletion mutants and point mutants are usefully utilized for epitope mapping, we often experience the difficulty of determining the mAb epitope against membrane proteins. We aimed to develop a novel method to determine the binding region of mAbs using epitope tag system. We first checked the reactivity of an anti-CD44 mAb (C44Mab-5) to several deletion mutants ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Teizo Asano Mika K Kaneko Yukinari Kato Source Type: research

Defucosylated Anti-Epidermal Growth Factor Receptor Monoclonal Antibody 134-mG < sub > 2a < /sub > -f Exerts Antitumor Activities in Mouse Xenograft Models of Dog Epidermal Growth Factor Receptor-Overexpressed Cells
In this study, the subclass of EMab-134 was converted from IgG1 to IgG2a (134-mG2a) and further defucosylated (134-mG2a-f) to facilitate antibody-dependent cellular cytotoxicity (ADCC). Although 134-mG2a-f was developed against hEGFR, it was shown to cross-react with dog EGFR (dEGFR) using flow cytometry. The dissociation constant (KD) of 134-mG2a-f against dEGFR-overexpressed CHO-K1 (CHO/dEGFR) cells was determined by flow cytometry to be 3.3 × 10-9 M, indicating that 134-mG2a-f possesses a high binding affinity to dEGFR. Analysis in vitro revealed that 134-mG2a-f contributed to high levels of ADCC and complement-de...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Ren Nanamiya Tomokazu Ohishi Junko Takei Takuro Nakamura Miyuki Yanaka Hideki Hosono Masaki Saito Teizo Asano Tomohiro Tanaka Masato Sano Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):149-155. doi: 10.1089/mab.2021.0010.ABSTRACTThe classic method for identifying the epitope that monoclonal antibodies (mAbs) bind uses deletion mutants and point mutants of the target protein. However, determining the epitope of mAbs-reactive membrane proteins is often challenging. We recently developed the RIEDL insertion for epitope mapping (REMAP) method to identify mAb-binding epitopes. Herein, we first checked the reactivity of an anti-epidermal growth factor receptor (EGFR) mAb (EMab-51) to several EGFR deletion mutants such as EGFR/dN152, EGFR/dN313, EGFR/dN370, ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Ren Nanamiya Masato Sano Teizo Asano Miyuki Yanaka Takuro Nakamura Masaki Saito Tomohiro Tanaka Hideki Hosono Nami Tateyama Mika K Kaneko Yukinari Kato Source Type: research

Development of a Novel Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-181 for Gastric Cancer
In this study, we have developed a novel anti-HER2 mAb, H2Mab-181 (IgG1, kappa), through the immunization of mice with a purified recombinant extracellular domain of HER2. H2Mab-181 can specifically and sensitively detect HER2 in both flow cytometry and Western blot applications in gastric cancer cell lines and can also be utilized in immunohistochemical analyses of gastric cancer tissues. Together, H2Mab-181 could be useful for the diagnosis and therapy in gastric cancers.PMID:34424764 | DOI:10.1089/mab.2021.0021 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Junko Takei Teizo Asano Tomohiro Tanaka Masato Sano Hideki Hosono Ren Nanamiya Nami Tateyama Masaki Saito Hiroyoshi Suzuki Hiroyuki Harada Mika K Kaneko Yukinari Kato Source Type: research

Harnessing Antigen Determinates for Target Identification
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):147-148. doi: 10.1089/mab.2021.29007.tke.NO ABSTRACTPMID:34424759 | DOI:10.1089/mab.2021.29007.tke (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Thomas Kieber-Emmons Source Type: research

An Anti-HER2 Monoclonal Antibody H < sub > 2 < /sub > Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells
Monoclon Antib Immunodiagn Immunother. 2021 Aug;40(4):184-190. doi: 10.1089/mab.2021.0025.ABSTRACTOverexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 23, 2021 Category: Microbiology Authors: Nami Tateyama Teizo Asano Tomokazu Ohishi Junko Takei Hideki Hosono Ren Nanamiya Tomohiro Tanaka Masato Sano Masaki Saito Manabu Kawada Mika K Kaneko Yukinari Kato Source Type: research