Development and Characterization of Anti-Sheep Podoplanin Monoclonal Antibodies PMab-253 and PMab-260.
This study develops and characterizes anti-sheep PDPN (sPDPN) mAbs using Cell-Based Immunization and Screening (CBIS) method. A RAP14 tag was added to the N-terminus of sPDPN, and an anti-RAP14 tag mAb (PMab-2) was used to measure the expression level of sPDPN in flow cytometry and Western blots. We immunized mice with sPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/sPDPN) cells and screened mAbs against sPDPN using flow cytometry. Two of the mAbs, PMab-253 (immunoglobulin M [IgM], kappa) and PMab-260 (IgM, kappa), detected CHO/sPDPN cells specifically using flow cytometry and Western blots. Both PMab-253 and PMab...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 19, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of an Anti-CD20 Monoclonal Antibody (C20Mab-60) for Immunohistochemical Analyses.
This study aims to establish sensitive and specific anti-CD20 mAbs suitable for broad application, such as flow cytometry, Western blotting, and immunohistochemical analyses. Using the Cell-Based Immunization and Screening (CBIS) method, all procedures were performed by utilizing CD20-stable transfectants, and a clone, C20Mab-60 (IgG2a, kappa), was developed. In flow cytometry, C20Mab-60 detected overexpression of CD20 in LN229 cell and endogenous CD20 in BALL-1 (a human B cell leukemia cell line) but did not react with CD20-knockout BALL-1 (BINDS-24), indicating specificity for CD20. In Western blotting, C20Mab-60 detecte...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 16, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of DhMab-1: An Antidiacylglycerol Kinase Monoclonal Antibody.
Authors: Sano M, Kaneko MK, Kato Y Abstract Diacylglycerol kinase (DGK) η is classified as a type II DGK and catalyzes diacylglycerol phosphorylation to produce phosphatidic acid. DGKη has been reported to be highly expressed in the hippocampus and cerebellum. Although a DGKη-specific monoclonal antibody (mAb) is necessary to reveal the association between the expression of DGKη and diseases, an anti-DGKη mAb for immunohistochemistry has not been developed. Recently, we established a specific antihuman DGKη (hDGKη) mAb, DhMab-1 (mouse IgG2a, kappa). For epitope mapping of DhMab-1, here w...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

An Antihuman Epidermal Growth Factor Receptor 2 Monoclonal Antibody (H2Mab-19) Exerts Antitumor Activity in Glioblastoma Xenograft Models.
In this study, we investigated the antitumor activity of H2Mab-19 in an LN229 glioblastoma xenograft model. H2Mab-19 showed high binding affinity (KD: 1.1 × 10-8 M) against LN229 cells. Furthermore, H2Mab-19 significantly reduced tumor development in an LN229 xenograft. These results suggest that treatment with H2Mab-19 may be a useful therapy for patients with HER2-expressing glioblastomas. PMID: 32644843 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibodies BA5-C5, EG6-H6, AG5-B8: Antigen Is Aliphatic Di- and Polyisocyanate.
Authors: PMID: 32579494 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 26, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

An Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-19 Exerts Antitumor Activity in Mouse Colon Cancer Xenografts.
In this study, a novel anti-HER2 monoclonal antibody (mAb), H2Mab-19 (IgG2b, κ) was characterized for its anticancer activity in colon cancers. H2Mab-19 showed both antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity activities against Caco-2, a colon cancer cell line. Furthermore, H2Mab-19 significantly reduced tumor development in a Caco-2 xenograft model. These results suggest that treatment with H2Mab-19 may be a useful therapy for patients with HER2-expressing colon cancers. PMID: 32552424 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of the Anti-Diacylglycerol Kinase Monoclonal Antibody DhMab-4 for Immunohistochemical Analysis.
In this study, we established a specific anti-human DGKη (hDGKη) mAb, DhMab-4 (mouse IgG2b, kappa). DhMab-4 strongly stained Purkinje cells of human cerebellum in immunohistochemistry analysis. For epitope mapping of DhMab-4, we produced deletion or point mutants of hDGKη and performed western blotting to determine the binding epitope of DhMab-4. DhMab-4 reacted with dN745 mutant but not with dN750 mutant, indicating that the N-terminus of the DhMab-4 epitope is located between amino acids 745 and 750. More detailed analysis using point mutants demonstrated that five mutants, that is, D747A, P748A, F749A, G750A...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 5, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

H2Mab-19 Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody Therapy Exerts Antitumor Activity in Pancreatic Cancer Xenograft Models.
In this study, we investigated the antitumor activities of H2Mab-19 in pancreatic cancer xenograft models. We selected MIA PaCa-2, a pancreatic cancer cell line which expresses HER2. H2Mab-19 showed high binding affinity (KD: 1.2 × 10-8 M) against MIA PaCa-2 cells. Furthermore, H2Mab-19 significantly reduced tumor development in a MIA PaCa-2 xenograft model. These results suggest that treatment with H2Mab-19 may be a useful therapy for patients with HER2-expressing pancreatic cancers. PMID: 32423281 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Thr80 of Sheep Podoplanin Is a Critical Epitope of the Antisheep Podoplanin Monoclonal Antibody: PMab-256.
Authors: Kato Y, Sano M, Asano T, Sayama Y, Kaneko MK Abstract An antisheep podoplanin (sPDPN) monoclonal antibody (mAb), PMab-256, has recently been established. PMab-256 shows positive immunostaining for lymphatic endothelial cells, lung type I alveolar cells, and kidney podocytes. PDPN possesses three platelet-aggregation-stimulating (PLAG) domains, PLAG1, PLAG2, and PLAG3, and a PLAG-like domain (PLD). The binding epitope of many anti-PDPN mAbs is located in PLAG domains or PLD. The purpose of this study is to determine the binding epitope of PMab-256. Analysis of sPDPN deletion mutants revealed that the N-term...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Detection of Lion Podoplanin Using the Antitiger Podoplanin Monoclonal Antibody PMab-231.
In this study, possible cross-reaction between available anti-PDPN mAbs and lioPDPN was examined. Immunohistochemical analysis showed that antitiger PDPN mAb PMab-231 (IgG2a, kappa) reacted with type I alveolar cells from lion lung, indicating that PMab-231 is useful for the detection of lioPDPN. PMID: 32401677 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 14, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of an Anti-Sheep Podoplanin Monoclonal Antibody PMab-256 for Immunohistochemical Analysis of Lymphatic Endothelial Cells.
In this study, we used the Cell-Based Immunization and Screening method for the development of anti-sPDPN mAbs. RAP14 tag was added to N-terminus of sPDPN, and anti-RAP14 tag mAb (PMab-2) was used to detect the expression level of sPDPN in flow cytometry and western blot. We immunized mice with sPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/sPDPN) cells and screened mAbs against sPDPN using flow cytometry. One of the mAbs, PMab-256 (IgG1, kappa), specifically detected CHO/sPDPN cells by flow cytometry and western blot. Furthermore, PMab-256 stained type I alveolar cells of lung, renal glomerulus and Bowman's caps...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 1, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA Immunization.
In this study, we report the generation and characterization of a monoclonal antibody (mAb) against the extracellular domain of hCD63 using DNA immunization. This mAb, clone 1C8-2B11, exhibits high performance for use in immunofluorescence and flow cytometry, and it has 10-fold higher affinity than the control antibody that is commercially available. mAb 1C8-2B11 has great potential to be a tool for research and clinical diagnosis. PMID: 32311306 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Molecular Characterization and Experimental Utility of Monoclonal Antibodies with Specificity for Aliphatic Di- and Polyisocyanates.
Authors: Wisnewski AV, Liu J Abstract Aliphatic di- and polyisocyanates are crucial chemical ingredients in many industrial processes and are a well-recognized cause of occupational asthma. Serologic detection of "chemical epitopes" in biological samples could serve as an exposure surveillance approach toward disease prevention, and thus we sought to generate aliphatic isocyanate-specific monoclonal antibodies (mAbs). Three hybridomas were generated from Balb/c mice immunized with a commercial product containing a combination of uretdione, homopolymer, and monomeric forms of hexamethylene diisocyanate (HD...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 19, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of Novel Mouse Monoclonal Antibodies Against Human CD19.
In this study, we first employed the Cell-Based Immunization and Screening (CBIS) method to produce anti-CD19 monoclonal antibodies using CD19-overexpressing cells for both immunization and screening. One established clone-C19Mab-1-proved to be useful in flow cytometry assays against lymphoma cell lines, such as BALL-1, P30/OHK, and Raji. Second, the extracellular domain of CD19 was immunized into mice, and enzyme-linked immunosorbent assays were performed for the first screening. One established clone-C19Mab-3-was determined to be useful for Western blotting and immunohistochemical analysis. Due to their complementary uti...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of PMab-241, a Lymphatic Endothelial Cell-Specific Anti-Bear Podoplanin Monoclonal Antibody.
Authors: Sayama Y, Sano M, Asano T, Furusawa Y, Takei J, Nakamura T, Yanaka M, Okamoto S, Handa S, Komatsu Y, Nakamura Y, Yanagawa M, Kaneko MK, Kato Y Abstract Anti-bear podoplanin (bPDPN) monoclonal antibodies (mAbs), including PMab-247 and PMab-241, have been previously established. Although PMab-247 has shown positive immunostaining for lymphatic endothelial cells (LECs), type I alveolar cells of the lung, and podocytes of the kidney, PMab-241 stains LECs but does not react with lung type I alveolar cells. PDPN possesses three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) and the PLA...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 4, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Peptide-Based Monoclonal Antibody Production Against SAG1 (P30) Protein of Toxoplasma gondii.
Authors: Naghi Vishteh M, Javadi Mamaghani A, Rashidi S, Seyyed Tabaei SJ, Jeddi-Tehrani M Abstract Toxoplasma gondii is an intracellular protozoan parasite that can infect a wide range of warm-blooded animals. Humans as an intermediate host are infected by ingesting infectious oocytes or tissue cysts, or passing through the placenta in pregnant women. The aim of this study is producing monoclonal antibodies against a synthetic peptide from (surface antigen 1 [SAG1] or P30) protein of T. gondii. A synthetic peptide from SAG1 (P30) protein was conjugated to Keyhole Limpet Hemocyanin (KLH (and then used for immunizat...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 30, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Expression Pattern of Plexin Domain Containing 2 in Human Hepatocellular Carcinoma.
In this study, we investigated the expression pattern of PLXDC2 in human hepatocellular carcinoma (HCC) tissues using a highly specific anti-PLXDC2 rabbit monoclonal antibody, which was recently developed. PLXDC2 was expressed in human HCC tissues including HCC cells, tumor vascular endothelial cells, and some infiltrating cells. The developed anti-PLXDC2 antibody allowed for highly specific and low background staining. Based on these current findings of PLXDC2 expression in human HCC tissues, the window may now be open to explore the role of PLXDC2 in human HCC. PMID: 32202949 [PubMed - as supplied by publisher] (Sour...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 25, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Safety of Romosozumab in Osteoporotic Men and Postmenopausal Woman: A Meta-Analysis and Systematic Review.
Authors: Mariscal G, Nuñez JH, Bhatia S, Barrios C, Domenech-Fernández P Abstract Sclerostin is a protein synthesized mainly by osteocytes whose function is to inhibit bone formation. A recent monoclonal antibody, Romosozumab, is able to block sclerostin. The aim of this meta-analysis is to compare the safety of Romosozumab with placebo and alendronate. Five randomized controlled trials that described the safety of Romosozumab in healthy men and postmenopausal women were analyzed. The measures to be compared were the number of adverse events and the number of serious adverse events. Specific results i...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Antibody-Drug Conjugates Using Mouse-Canine Chimeric Anti-Dog Podoplanin Antibody Exerts Antitumor Activity in a Mouse Xenograft Model.
In this study, we developed an ADC, P38B-DM1, using the mouse-canine chimeric anti-dPDPN antibody, P38B as the antibody, a peptide linker, and emtansine as the payload using the chemical conjugation by affinity peptide (CCAP) method. We investigated its cytotoxicity against dPDPN-overexpressed Chinese hamster ovary (CHO/dPDPN) cells in vitro and its antitumor activity using a mouse xenograft model of CHO/dPDPN cells. P38B-DM1 showed cytotoxicity to CHO/dPDPN cells in a dose-dependent manner in vitro. Furthermore, P38B-DM1 exhibited higher antitumor activity than P38B in the mouse xenograft model. These results suggest that...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 18, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of a Monoclonal Antibody That Recognizes Cysteine-Rich Domain 1 of Human CD271.
Authors: Morita S, Mochizuki M, Shibuya-Takahashi R, Nakamura-Shima M, Yamazaki T, Imai T, Asada Y, Matsuura K, Kawamura S, Yamaguchi K, Yasuda J, Sugamura K, Katori Y, Satoh K, Tamai K Abstract CD271 is a common receptor for all neurotrophins that is localized to neurons, endothelial cells, and the basal layer of the epithelium in normal tissue. Recently, we and others reported that CD271 plays essential roles in the development of squamous cell carcinoma, especially in tumor-initiating cells. Since little is known about how CD271 regulates cancer cell initiation and proliferation, antibodies that recognize differ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Against Stromal Cell-Derived Factor 2.
Authors: PMID: 32069134 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody MAb 44A11 Against Sodium Nifurstyrenate.
Authors: PMID: 32069135 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Production and Characterization of Monoclonal Antibodies Against Gp Protein of Ebola Virus.
Authors: Yu M, Ye J, Cao S, Liu X, Chen Z Abstract The DNA fragment encoding predicted main antigenic region, aa 1-300 on Gp protein of Ebola virus (EBOV) was cloned into the vector pGEX-KG. The recombinant GST-tagged Gp-300 was expressed in Escherichia coli BL21 (DE3) by induction with 1 mM isopropyl-1-thio-b-d-galactoside and purified by dialysis. Four monoclonal antibodies (mAbs) named 1C4, 2A3, 2G7, and 2H9 against Gp protein were generated by fusing mouse myeloma cell line SP2/0 with spleen lymphocytes from Gp-300 protein-immunized mice. The activity of the mAbs was then characterized by enzyme-linked i...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 2, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Analysis of an Anti-Whale Podoplanin Monoclonal Antibody, PMab-237, Using Flow Cytometry.
In this study, deletion mutants and point mutants of wPDPN with N-terminal RIEDL tag were produced to analyze the PMab-237 epitope using flow cytometry. The analysis of deletion mutants showed that the N-terminus of the PMab-237 epitope exists between the 80th amino acid (AA) and the 85th AA of wPDPN. In addition, the analysis of point mutants demonstrated that the critical epitope of PMab-237 includes Leu82 and Thr84 of wPDPN, indicating that the PMab-237 epitope is located in the PLD of wPDPN. PMID: 31934820 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 15, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2.
In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells. PMID: 31916900 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 11, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Characterization of a Novel Bispecific Antibody That Activates T Cells In Vitro and Slows Tumor Growth In Vivo.
Authors: Chornoguz O, Leettola CN, Leander K, Brosnan K, Emmell E, Chiu ML, Santulli-Marotto S Abstract Although CD3 T cell redirecting antibodies have been successfully utilized for the treatment of hematological malignancies (blinatumomab), the T cell signaling pathways induced by these molecules are incompletely understood. To gain insight into the mechanism of action for T cell redirection antibodies, we created a novel murine CD3xEpCAM bispecific antibody that incorporates a silent Fc to dissect function and signaling of murine CD8 OT1 T cells upon stimulation. T cell-mediated cytotoxicity, cytokine secretion,...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Seroepidemiology Study of Helicobacter pylori Infection and Its Effect on Cardiovascular Diseases in Karaj, Iran.
Authors: Afsharpooyan S, Mohammadian T Abstract Helicobacter pylori infection occurs throughout the world between countries and races. A seroprevalence study of H. pylori infection in the occurrence of atherosclerosis (ASc) has been considered to be the main cause of cardiovascular diseases (CVDs) in part of Iran. From 600 volunteers, 97 serum samples were selected and tested for the presence of H. pylori infection. Positive and negative H. pylori infection samples were then selected for Chlamydia pneumonia and cytomegalovirus infection. Their serum levels for high-sensitive C-reactive protein (hs-CRP) and IL-6 tit...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 21, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein.
This study shows that, due to the advantages such as multi-epitope-binding ability, camelid pAbs are potent to efficiently apply for molecular detection of CTLA-4 receptors in fundamental antibody-based researches such as IHC. PMID: 31718460 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-241 Specifically Detects Bear Podoplanin of Lymphatic Endothelial Cells in the Lung of Brown Bear.
In this study, we showed that a novel anti-bPDPN mAb, PMab-241 stained only lymphatic endothelial cells, not type I alveolar cells of the lung in immunohistochemical analyses. These findings suggest that PMab-241 could be useful for staining lymphatic endothelial cells specifically in the bear lung tissues. PMID: 31721640 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Epitope of PMab-210 Is Located in Platelet Aggregation-Stimulating Domain-3 of Pig Podoplanin.
In this study, deletion mutants or point mutants of pPDPN were produced for analyzing the PMab-210 epitope using flow cytometry. The analysis of deletion mutants showed that N-terminus of PMab-210 epitope exists between 45th amino acid (aa) and 50th aa of pPDPN. In addition, the analysis of point mutants demonstrated that the critical epitope of PMab-210 could include Glu47, Asp48, Tyr49, Thr50, and Val51 of pPDPN, indicating that PMab-210 epitope is located in PLAG3 domain of pPDPN. PMID: 31663836 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 1, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Analysis of an Antihorse Podoplanin Monoclonal Antibody PMab-219.
In this study, a series of deletion mutants or point mutants of horPDPN were produced for analyzing the PMab-219 epitope using flow cytometry. The analysis of deletion mutants showed that N-terminus of PMab-219 epitope exists between 55th amino acid (aa) and 60th aa of horPDPN. Furthermore, the analysis of point mutants demonstrated that the critical epitope of PMab-219, which was developed by CBIS method, could include Val59, Arg61, Ser62, and Thr63 of horPDPN, indicating that PMab-219 epitope is independent of PLAG domain or PLD of horPDPN. PMID: 31638470 [PubMed - as supplied by publisher] (Source: Monoclonal Antibo...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 23, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Monoclonal Antibody PMab-233 Against Tasmanian Devil Podoplanin.
Authors: Sano M, Kaneko MK, Kato Y Abstract The highly O-glycosylated membrane glycoprotein podoplanin (PDPN) is frequently overexpressed in several malignant cancers, such as oral cancer, lung cancer, germinal neoplasia, mesothelioma, and brain tumor. The expression of PDPN is strongly associated with cancer progression and poor prognosis. PDPN possesses three tandem repeats of platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) and PLAG-like domain (PLD), and binds to C-type lectin-like receptor 2 (CLEC-2) on platelets, followed by PDPN-mediated platelet aggregation. We have previously estab...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 19, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of PMab-225 an Anti-Alpaca Podoplanin Monoclonal Antibody Using Flow Cytometry.
Authors: Sayama Y, Sano M, Furusawa Y, Kaneko MK, Kato Y Abstract Podoplanin (PDPN) is a mucin-type membrane glycoprotein, and possesses three platelet aggregation-stimulating (PLAG) domains: PLAG1, PLAG2, and PLAG3 at the N-terminus of PDPN, and one or two PLAG-like domains (PLDs) in the middle of PDPN. PDPN is expressed on normal tissues, such as podocytes of the kidney and type I alveolar cells of the lung, and is also overexpressed in numerous malignant cancers. Previously, we reported a novel anti-alpaca podoplanin (aPDPN) monoclonal antibody (mAb), PMab-225, using Cell-Based Immunization and Screening (CBIS) ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 16, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Suppression of Disease-Associated B Lymphocytes by GAD65 Epitope-Carrying Protein-Engineered Molecules in a Streptozotocin-Induced Mouse Model of Diabetes.
Authors: Manoylov IK, Boneva GV, Doytchinova IA, Mihaylova NM, Tchorbanov AI Abstract Type 1 diabetes mellitus is an autoimmune syndrome defined by the presence of autoreactive T and B cells, which results in destruction of insulin-producing beta cells. Autoantibodies against GAD65 (glutamic acid decarboxylase 65)-a membrane-bound enzyme on pancreatic beta cells, contribute to beta cells' destruction and the loss of pancreatic functions. Mouse FcγRIIb on B lymphocytes possesses an inhibitory effect on the activity of these cells. We hypothesized that it may be possible to suppress GAD65-specific B cells in mi...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Targeting a Conserved N-Terminal Epitope on 2Apro of Enteroviruses.
In this study, we achieved a murine mAb, named 5A3, specifically toward EVA71 2Apro by using the traditional hybridoma technique. The mAb 5A3 recognizes 2Apro of both EVs A and B species, which was demonstrated by indirect fluorescent assay and Western blotting. Furthermore, a conserved N-terminal epitope on 2Apro recognized by mAb 5A3 was defined by using an overlapping peptide-based enzyme-linked immunosorbent assay (ELISA). Therefore, the unique mAb targeting conserved EVs 2Apro can be used as an important tool during both identifying the causative agent of HFMD and elucidating the pathological mechanism of HFMD. PM...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Rapid Detection of Staphylococcal Enterotoxin-B by Lateral Flow Assay.
Authors: Hnasko R, Lin AV, McGarvey JA Abstract A cohort of monoclonal antibodies (mAbs) were generated against Staphylococcal enterotoxin-B (SEB) and selected by double sandwich enzyme-linked immunosorbent assay (ELISA) for solution capture of the toxin. Clonal hybridoma cell lines were established and a pair of anti-SEB mAbs selected for the development of a sandwich ELISA. Immobilized 3D6 mAb (IgG1, kappa) when paired with 4C9 mAb (IgG1, kappa) conjugated to horseradish peroxidase generates a typical dose-response curve with an EC50 of 24.8 ng/mL for purified SEB using chemiluminescent detection. These mA...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Anti-Bear Podoplanin Monoclonal Antibody PMab-247.
In this study, we aimed to investigate the epitope of PMab-247 using enzyme-linked immunosorbent assay and IHC analyses. The results revealed that the critical epitopes of PMab-247 are Asp76, Arg78, Glu80, and Arg82 of bPDPN. The Glu80 and Arg82 are included in PLD of bPDPN. The findings of our study can be applied to the production of more functional anti-bPDPN mAbs. PMID: 31535919 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - September 21, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of Monoclonal Antibodies Against Glycophorin A Applicable for Blood Sample Processing.
In conclusion, the anti-GPA mAbs are useful tools in developing a quick and easy way to separate blood plasma from whole blood for clinical tests, and in developing bi-specific antibodies for other clinical applications. PMID: 31486711 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - September 6, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Antipig Podoplanin Monoclonal Antibody PMab-213.
Authors: Yamada S, Itai S, Furusawa Y, Kaneko MK, Kato Y Abstract Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on lymphatic endothelial cells, podocytes of the kidneys, and type I alveolar cells of the lungs. PDPN, a platelet aggregation-inducing factor, comprises three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) in the N-terminus and PLAG-like domains in the middle of the PDPN protein. We have previously reported a mouse antipig PDPN (pPDPN) monoclonal antibody (mAb) clone, PMab-213, which was developed using the Cell-Based Immunization and Screening (CBIS...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 30, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Efficient Screening and Design of Variable Domain of Heavy Chain Antibody Ligands Through High Throughput Sequencing for Affinity Chromatography to Purify Fab Fragments.
Authors: Rafique A, Satake K, Kishimoto S, Hasan Khan K, Kato DI, Ito Y Abstract To design an affinity ligand for purification of antigen-binding fragment (Fab) antibody, variable domain of heavy chain antibody (VHH) phage libraries were constructed from Fab-immunized Alpaca and subjected to biopanning against Fabs. To find the specific binders, we directly applied high-throughput sequencing (HTS) analysis of the VHH sequences in the panned phages on next-generation sequencer. The efficiently enriched sequences were aligned for construction of the phylogenetic tree to be categorized into five groups. VHHs from thre...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 16, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Characterization of Anti-Goat Podoplanin Monoclonal Antibody PMab-235 Using Immunohistochemistry Against Goat Tissues.
In this study, we investigated the epitopes of PMab-235 using enzyme-linked immunosorbent assay and immunohistochemistry. The results revealed that the critical epitope of PMab-235 produced by CBIS method is Arg75, Leu78, and Pro79 of gPDPN, which is included in PLD. The findings of our study can be applied to the production of more functional anti-gPDPN mAbs. PMID: 31403389 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

DzMab-1: Anti-Human Diacylglycerol Kinase ΞΆ Monoclonal Antibody for Immunocytochemistry.
In this study, we developed a novel anti-human DGKζ monoclonal antibody, DzMab-1, which is very advantageous in immunocytochemistry of human cultured cells. PMID: 31403910 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody KIF20B(10C7), MPP1.
Authors: PMID: 31403911 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Anti-Diacylglycerol Kinase Zeta Monoclonal Antibody DzMab-1 for Immunohistochemical Analyses.
In this study, we characterized the binding epitope of DzMab-1 using Western blotting, and found that Met1 and Pro3 residues of hDGKζ are important for facilitating DzMab-1 binding to hDGKζ. Furthermore, DzMab-1 was shown to be useful for immunohistochemical analyses for formalin-fixed paraffin-embedded HeLa cells. These findings could be applied for the production of more functional anti-hDGKζ mAbs. PMID: 31355694 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 31, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Dynamic Changes in Soluble CD30 and Regulatory T Cells Before and After Solid Organ Transplantations: A Pilot Study.
Authors: Terzieva V, Mihova A, Altankova I, Velikova T, Donchev D, Uzunova J, Goncharov A, Jurukova N, Georgieva V, Yordanova E, Sekulovski M, Chalamanov O, Spassov L Abstract Among multiple parameters, applied in the immunologic monitoring of transplantation, the levels of serum soluble CD30 (sCD30) and peripheral regulatory T cells (Tregs) are very promising. These are relatively new biomarkers, considered to reflect immune activation and tolerance in solid organ transplantation. Results are shown here from a preliminary study on the relevance of sCD30 and Tregs in the monitoring of the early post-transplantation...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 31, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis.
In this study, immunohistochemical analyses showed that PMab-247 strongly detected bPDPN, which is expressed in type I alveolar cells and lymphatic endothelial cells of bear lung and podocytes of bear kidney. These findings suggest that PMab-247 could be useful for pathophysiological analyses using immunohistochemistry. PMID: 31313968 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 18, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of a Potent Tumor Necrosis Factor-Alpha-Blocking Agent.
Authors: Jameei A, Nagarajan D, Sarikhani M, Chandra N, Karande AA Abstract Tumor necrosis factor-α (TNFα), one of the major proinflammatory cytokines, plays a key role in an effective immune response. However, the chronic presence of TNFα can lead to several inflammatory disorders, such as rheumatoid arthritis, psoriasis, Crohn's disease, etc. Inhibition of TNFα by pharmacological inhibitors or antibodies has proven to be effective in palliative treatment to some extent. The aim of this study was to develop an anti-TNFα antibody, which may be used as a therapeutic option to inhibit TN...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 17, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

A Monoclonal Antibody to M-Phase Phosphoprotein 1/Kinesin-Like Protein KIF20B.
Authors: Fritzler MJ, Brown RD, Zhang M Abstract Kinesin-like protein KIF20B, originally named M-phase phosphoprotein 1 (MPP1), is a plus-end-directed kinesin-related protein that exhibits in vitro microtubule-binding and -bundling properties as well as microtubule-stimulated ATPase activity. It has been characterized as a slow molecular motor that moves toward the plus-end of microtubules. Human autoantibodies directed against KIF20B have been described in up to 25% of patients with idiopathic ataxia and less commonly in other neuropathies and autoinflammatory conditions. One of the limitations of research into th...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 3, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-41 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer.
In this study, we developed a novel anti-HER2 monoclonal antibody (mAb), H2Mab-41 (IgG2b, kappa), and the antitumor activity of H2Mab-41 was investigated using mouse xenograft models. Caco-2 cells (human colon cancer cell line), which expresses HER2, were subcutaneously implanted into the flanks of nude mice. H2Mab-41 and control mouse IgG were injected three times into the peritoneal cavity of mice. H2Mab-41 significantly reduced tumor development of Caco-2 xenograft in comparison with the control mouse IgG on days 5, 8, 11, 15, and 19. Taken together, these results suggest that H2Mab-41 is useful for antibody therapy aga...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 16, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Application of Rat Monoclonal Antibodies Specific for a Human Blood Coagulation Protein: von Willebrand Factor.
In conclusion, the development of VWF-specific mAbs would be useful in the diagnosis of VWD and AVWS. PMID: 31192778 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 14, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research