Epitope Mapping of Monoclonal Antibody PMab-233 Against Tasmanian Devil Podoplanin.
Authors: Sano M, Kaneko MK, Kato Y Abstract The highly O-glycosylated membrane glycoprotein podoplanin (PDPN) is frequently overexpressed in several malignant cancers, such as oral cancer, lung cancer, germinal neoplasia, mesothelioma, and brain tumor. The expression of PDPN is strongly associated with cancer progression and poor prognosis. PDPN possesses three tandem repeats of platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) and PLAG-like domain (PLD), and binds to C-type lectin-like receptor 2 (CLEC-2) on platelets, followed by PDPN-mediated platelet aggregation. We have previously estab...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 19, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of PMab-225 an Anti-Alpaca Podoplanin Monoclonal Antibody Using Flow Cytometry.
Authors: Sayama Y, Sano M, Furusawa Y, Kaneko MK, Kato Y Abstract Podoplanin (PDPN) is a mucin-type membrane glycoprotein, and possesses three platelet aggregation-stimulating (PLAG) domains: PLAG1, PLAG2, and PLAG3 at the N-terminus of PDPN, and one or two PLAG-like domains (PLDs) in the middle of PDPN. PDPN is expressed on normal tissues, such as podocytes of the kidney and type I alveolar cells of the lung, and is also overexpressed in numerous malignant cancers. Previously, we reported a novel anti-alpaca podoplanin (aPDPN) monoclonal antibody (mAb), PMab-225, using Cell-Based Immunization and Screening (CBIS) ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 16, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Suppression of Disease-Associated B Lymphocytes by GAD65 Epitope-Carrying Protein-Engineered Molecules in a Streptozotocin-Induced Mouse Model of Diabetes.
Authors: Manoylov IK, Boneva GV, Doytchinova IA, Mihaylova NM, Tchorbanov AI Abstract Type 1 diabetes mellitus is an autoimmune syndrome defined by the presence of autoreactive T and B cells, which results in destruction of insulin-producing beta cells. Autoantibodies against GAD65 (glutamic acid decarboxylase 65)-a membrane-bound enzyme on pancreatic beta cells, contribute to beta cells' destruction and the loss of pancreatic functions. Mouse FcγRIIb on B lymphocytes possesses an inhibitory effect on the activity of these cells. We hypothesized that it may be possible to suppress GAD65-specific B cells in mi...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Targeting a Conserved N-Terminal Epitope on 2Apro of Enteroviruses.
In this study, we achieved a murine mAb, named 5A3, specifically toward EVA71 2Apro by using the traditional hybridoma technique. The mAb 5A3 recognizes 2Apro of both EVs A and B species, which was demonstrated by indirect fluorescent assay and Western blotting. Furthermore, a conserved N-terminal epitope on 2Apro recognized by mAb 5A3 was defined by using an overlapping peptide-based enzyme-linked immunosorbent assay (ELISA). Therefore, the unique mAb targeting conserved EVs 2Apro can be used as an important tool during both identifying the causative agent of HFMD and elucidating the pathological mechanism of HFMD. PM...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Rapid Detection of Staphylococcal Enterotoxin-B by Lateral Flow Assay.
Authors: Hnasko R, Lin AV, McGarvey JA Abstract A cohort of monoclonal antibodies (mAbs) were generated against Staphylococcal enterotoxin-B (SEB) and selected by double sandwich enzyme-linked immunosorbent assay (ELISA) for solution capture of the toxin. Clonal hybridoma cell lines were established and a pair of anti-SEB mAbs selected for the development of a sandwich ELISA. Immobilized 3D6 mAb (IgG1, kappa) when paired with 4C9 mAb (IgG1, kappa) conjugated to horseradish peroxidase generates a typical dose-response curve with an EC50 of 24.8 ng/mL for purified SEB using chemiluminescent detection. These mA...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Anti-Bear Podoplanin Monoclonal Antibody PMab-247.
In this study, we aimed to investigate the epitope of PMab-247 using enzyme-linked immunosorbent assay and IHC analyses. The results revealed that the critical epitopes of PMab-247 are Asp76, Arg78, Glu80, and Arg82 of bPDPN. The Glu80 and Arg82 are included in PLD of bPDPN. The findings of our study can be applied to the production of more functional anti-bPDPN mAbs. PMID: 31535919 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - September 21, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of Monoclonal Antibodies Against Glycophorin A Applicable for Blood Sample Processing.
In conclusion, the anti-GPA mAbs are useful tools in developing a quick and easy way to separate blood plasma from whole blood for clinical tests, and in developing bi-specific antibodies for other clinical applications. PMID: 31486711 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - September 6, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Antipig Podoplanin Monoclonal Antibody PMab-213.
Authors: Yamada S, Itai S, Furusawa Y, Kaneko MK, Kato Y Abstract Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on lymphatic endothelial cells, podocytes of the kidneys, and type I alveolar cells of the lungs. PDPN, a platelet aggregation-inducing factor, comprises three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) in the N-terminus and PLAG-like domains in the middle of the PDPN protein. We have previously reported a mouse antipig PDPN (pPDPN) monoclonal antibody (mAb) clone, PMab-213, which was developed using the Cell-Based Immunization and Screening (CBIS...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 30, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Efficient Screening and Design of Variable Domain of Heavy Chain Antibody Ligands Through High Throughput Sequencing for Affinity Chromatography to Purify Fab Fragments.
Authors: Rafique A, Satake K, Kishimoto S, Hasan Khan K, Kato DI, Ito Y Abstract To design an affinity ligand for purification of antigen-binding fragment (Fab) antibody, variable domain of heavy chain antibody (VHH) phage libraries were constructed from Fab-immunized Alpaca and subjected to biopanning against Fabs. To find the specific binders, we directly applied high-throughput sequencing (HTS) analysis of the VHH sequences in the panned phages on next-generation sequencer. The efficiently enriched sequences were aligned for construction of the phylogenetic tree to be categorized into five groups. VHHs from thre...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 16, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Characterization of Anti-Goat Podoplanin Monoclonal Antibody PMab-235 Using Immunohistochemistry Against Goat Tissues.
In this study, we investigated the epitopes of PMab-235 using enzyme-linked immunosorbent assay and immunohistochemistry. The results revealed that the critical epitope of PMab-235 produced by CBIS method is Arg75, Leu78, and Pro79 of gPDPN, which is included in PLD. The findings of our study can be applied to the production of more functional anti-gPDPN mAbs. PMID: 31403389 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

DzMab-1: Anti-Human Diacylglycerol Kinase ζ Monoclonal Antibody for Immunocytochemistry.
In this study, we developed a novel anti-human DGKζ monoclonal antibody, DzMab-1, which is very advantageous in immunocytochemistry of human cultured cells. PMID: 31403910 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody KIF20B(10C7), MPP1.
Authors: PMID: 31403911 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Anti-Diacylglycerol Kinase Zeta Monoclonal Antibody DzMab-1 for Immunohistochemical Analyses.
In this study, we characterized the binding epitope of DzMab-1 using Western blotting, and found that Met1 and Pro3 residues of hDGKζ are important for facilitating DzMab-1 binding to hDGKζ. Furthermore, DzMab-1 was shown to be useful for immunohistochemical analyses for formalin-fixed paraffin-embedded HeLa cells. These findings could be applied for the production of more functional anti-hDGKζ mAbs. PMID: 31355694 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 31, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Dynamic Changes in Soluble CD30 and Regulatory T Cells Before and After Solid Organ Transplantations: A Pilot Study.
Authors: Terzieva V, Mihova A, Altankova I, Velikova T, Donchev D, Uzunova J, Goncharov A, Jurukova N, Georgieva V, Yordanova E, Sekulovski M, Chalamanov O, Spassov L Abstract Among multiple parameters, applied in the immunologic monitoring of transplantation, the levels of serum soluble CD30 (sCD30) and peripheral regulatory T cells (Tregs) are very promising. These are relatively new biomarkers, considered to reflect immune activation and tolerance in solid organ transplantation. Results are shown here from a preliminary study on the relevance of sCD30 and Tregs in the monitoring of the early post-transplantation...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 31, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis.
In this study, immunohistochemical analyses showed that PMab-247 strongly detected bPDPN, which is expressed in type I alveolar cells and lymphatic endothelial cells of bear lung and podocytes of bear kidney. These findings suggest that PMab-247 could be useful for pathophysiological analyses using immunohistochemistry. PMID: 31313968 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 18, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of a Potent Tumor Necrosis Factor-Alpha-Blocking Agent.
Authors: Jameei A, Nagarajan D, Sarikhani M, Chandra N, Karande AA Abstract Tumor necrosis factor-α (TNFα), one of the major proinflammatory cytokines, plays a key role in an effective immune response. However, the chronic presence of TNFα can lead to several inflammatory disorders, such as rheumatoid arthritis, psoriasis, Crohn's disease, etc. Inhibition of TNFα by pharmacological inhibitors or antibodies has proven to be effective in palliative treatment to some extent. The aim of this study was to develop an anti-TNFα antibody, which may be used as a therapeutic option to inhibit TN...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 17, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

A Monoclonal Antibody to M-Phase Phosphoprotein 1/Kinesin-Like Protein KIF20B.
Authors: Fritzler MJ, Brown RD, Zhang M Abstract Kinesin-like protein KIF20B, originally named M-phase phosphoprotein 1 (MPP1), is a plus-end-directed kinesin-related protein that exhibits in vitro microtubule-binding and -bundling properties as well as microtubule-stimulated ATPase activity. It has been characterized as a slow molecular motor that moves toward the plus-end of microtubules. Human autoantibodies directed against KIF20B have been described in up to 25% of patients with idiopathic ataxia and less commonly in other neuropathies and autoinflammatory conditions. One of the limitations of research into th...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 3, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-41 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer.
In this study, we developed a novel anti-HER2 monoclonal antibody (mAb), H2Mab-41 (IgG2b, kappa), and the antitumor activity of H2Mab-41 was investigated using mouse xenograft models. Caco-2 cells (human colon cancer cell line), which expresses HER2, were subcutaneously implanted into the flanks of nude mice. H2Mab-41 and control mouse IgG were injected three times into the peritoneal cavity of mice. H2Mab-41 significantly reduced tumor development of Caco-2 xenograft in comparison with the control mouse IgG on days 5, 8, 11, 15, and 19. Taken together, these results suggest that H2Mab-41 is useful for antibody therapy aga...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 16, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Application of Rat Monoclonal Antibodies Specific for a Human Blood Coagulation Protein: von Willebrand Factor.
In conclusion, the development of VWF-specific mAbs would be useful in the diagnosis of VWD and AVWS. PMID: 31192778 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 14, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Late Postpregnancy Modifications in the Subset of Peripheral Natural T Regulatory Cells in Healthy Women.
In this study, we investigate the population of Tregs and, in particular, the natural Tregs (nTregs) in healthy women divided into three groups according to the number of previous pregnancies, if any (Gr.1-one pregnancy, Gr.2-≥2 pregnancies, and Gr.0-no pregnancy). The overall analysis showed similar proportions in the entire Treg pool and nTregs (FoxP3+CD45RA+) in all the three groups (p > 0.05). However, the age-related trend of CD25+ nTregs was found to be different in parous and nonparous women. Analysis of phosphorylated ERK1/2, an important signaling molecule in T cell maintenance, showed a signif...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 14, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Immunohistochemical Analysis of the Harbor Porpoise Using Antipodoplanin Antibody PMab-237.
Authors: Yamada S, Itai S, Nakamura T, Takei J, Sano M, Konnai S, Kobayashi A, Nakagun S, Kobayashi Y, Kaneko MK, Kato Y Abstract Podoplanin (PDPN)/T1 alpha is known as a specific marker of lymphatic endothelial cells and type I alveolar cells. Sensitive and specific monoclonal antibodies (mAbs) for PDPN are needed for immunohistochemical analyses. Recently, we developed an anticetacean PDPN mAb, PMab-237. Herein, immunohistochemical analyses showed that PMab-237 strongly detected pulmonary type I alveolar cells, renal podocytes, and lymphatic endothelial cells of the harbor porpoise. These findings suggest that PM...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 6, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of an Anticetacean Podoplanin Monoclonal Antibody PMab-237 for Immunohistochemical Analysis.
In this study, we immunized mice with wPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/wPDPN) cells, and screened mAbs against wPDPN using flow cytometry. One of the mAbs, PMab-237 (IgG1, kappa), specifically detected CHO/wPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-237 for the functional analyses of wPDPN. PMID: 31161965 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 6, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Antihuman Diacylglycerol Kinase Gamma Monoclonal Antibody DgMab-6.
In this study, we characterized the binding epitope of DgMab-6 using Western blotting, and Glu12 is important for facilitating the DgMab-6 binding to the DGKγ protein. These results might lead to further development for sensitive and specific mAbs against DGKγ. PMID: 31149888 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 4, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Anti-Diacylglycerol Kinase ζ Monoclonal Antibody for the Detection of T Cells by Immunohistochemical Analyses.
In this study, we performed immunohistochemical analysis using a rabbit anti-DGKζ monoclonal antibody (mAb) (clone EPR22040-80) against tissues from the tonsils of a patient with oropharyngeal squamous cell carcinoma. We demonstrated that many DGKζ-expressing T cells are localized in the tonsils. We further characterized the binding epitope using an enzyme-linked immunosorbent assay and found that Pro790, Gln791, Gly792, and Leu795 residues of DGKζ are important for facilitating anti-DGKζ mAb binding to DGKζ. This anti-DGKζ mAb could be valuable in immunohistochemical analyses in determining t...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 22, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Anti-Tiger Podoplanin Monoclonal Antibody PMab-231.
This study aimed to investigate the epitopes of PMab-231, which was developed by CBIS method, using enzyme-linked immunosorbent assay. The results revealed that the critical epitopes of PMab-231 are Glu29, Asp30, Asp31, Ile32, Met33, Thr34, Pro35, Gly36, and Glu38 of tigPDPN, which is corresponding to PLAG1/2. The findings of our study can be applied to the production of more functional anti-tigPDPN mAbs. PMID: 31112076 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 22, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment and Epitope Mapping of Anti-Diacylglycerol Kinase α Monoclonal Antibody DaMab-8 for Immunohistochemical Analyses.
Establishment and Epitope Mapping of Anti-Diacylglycerol Kinase α Monoclonal Antibody DaMab-8 for Immunohistochemical Analyses. Monoclon Antib Immunodiagn Immunother. 2019 May 17;: Authors: Sano M, Kaneko MK, Suzuki H, Kato Y Abstract Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). DGKα, 1 of the 10 DGK isozymes, is involved in T cell function. In the present study, we describe a specific monoclonal antibody DaMab-8 (mouse IgG1, kappa) against DGKα, which is extremely useful for performing immunohistochemical analysis for T cells in oropharyngeal ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 18, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Upregulation of Natural Killer Cells Proliferation by Cytokine Stimulation.
Authors: Pencheva-Demireva M, El-Darawish Y, Kavaldzhieva K, Mladenov N, Lazarov V, Dimitrova-Dikanarova D, Markova T, Nikolov R, Okamura H Abstract Natural killer (NK) cells can discriminate between normal and cancer cells and are known to directly recognize and kill malignant cells or induce apoptosis. Thus, activation of NK cells is considered as a promising strategy for cancer treatment. However, clinical application has been somewhat limited because of difficulties in the preparation of sufficient number of highly cytotoxic/activated NK cells in vitro. We used cytokine stimulation to provide a suitable environ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 24, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Recombinant Expression of Zinc Transporter SLC39A6 and Its Functional Antibody Production.
In conclusion, this protein can be used for animal immunization toward preparation of a new monoclonal antibody that can be introduced as a drug in the treatment of breast cancer. PMID: 31009334 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 24, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Enhancing Natural Killer and CD8+ T Cell-Mediated Anticancer Cytotoxicity and Proliferation of CD8+ T Cells with HLA-E Monospecific Monoclonal Antibodies.
Authors: Ravindranath MH, Filippone EJ, Devarajan A, Asgharzadeh S Abstract Cytotoxic NK/CD8+ T cells interact with MHC-I ligands on tumor cells through either activating or inhibiting receptors. One of the inhibitory receptors is CD94/NKG2A. The NK/CD8+ T cell cytotoxic capability is lost when tumor-associated human leukocyte antigen, HLA-E, binds the CD94/NKG2A receptor, resulting in tumor progression and reduced survival. Failure of cancer patients to respond to natural killer (NK) cell therapies could be due to HLA-E overexpression in tumor tissues. Preventing the inhibitory receptor-ligand interaction by eithe...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 24, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of a Monoclonal Antibody PMab-231 for Tiger Podoplanin.
In this study, we immunized mice with tigPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/tigPDPN) and screened hybridomas producing mAbs against tigPDPN using flow cytometry. One of the mAbs, PMab-231 (IgG2a, kappa), specifically detected CHO/tigPDPN cells using flow cytometry as well as recognized tigPDPN protein using western blotting. In addition, PMab-231 was found to cross-react with cat PDPN (cPDPN). The dissociation constants (KD) of PMab-231 for CHO/tigPDPN and CHO/cPDPN cells were determined to be 1.2 × 10-8 and 1.9 × 10-8, respectively, indicating moderate aff...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 24, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Commentary: Metabolism Impact on Immunotherapy.
Authors: Kieber-Emmons T PMID: 31009337 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 24, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

CD28 Superagonistic Activation of T Cells Induces a Tumor Cell-Like Metabolic Program.
In this study, we show that human CD4+ TEMs stimulated with CD28SA adopt a metabolic program similar to those of tumor cells with enhanced glucose utilization, lipid biosynthesis, and proliferation in hypoxic conditions. Identification of metabolic profiles underlying hyperactive T cell activation would provide a platform to test safety of immunostimulatory antibodies. PMID: 31009338 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 24, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Anti-CD133 Monoclonal Antibody CMab-43 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer.
This study aimed to investigate the antitumor activity of CMab-43. Caco-2 cells (human colon cancer cell line) were subcutaneously implanted into the flanks of nude mice. CMab-43 and control mouse IgG were injected three times into the peritoneal cavity of mice. Tumor formation was observed in the control and CMab-43-treated mice of Caco-2 xenograft models. CMab-43 significantly reduced tumor development of Caco-2 xenograft in comparison with the control mouse IgG on days 12, 14, and 17. Our results cumulatively suggest that CMab-43 is useful for antibody therapy against CD133-expressing colon cancers. PMID: 30969150 [...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 11, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of the Antihorse Podoplanin Monoclonal Antibody PMab-202.
In this study, enzyme-linked immunosorbent assay and flow cytometry were used to identify the epitope of PMab-202. We found that the critical epitopes of PMab-202 include Lys64, Thr66, and Phe70 of horPDPN. We believe that our findings can be applied in the production of more functional anti-horPDPN mAbs. PMID: 30939066 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 4, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-213: A Monoclonal Antibody for Immunohistochemical Analysis Against Pig Podoplanin.
Authors: Kato Y, Yamada S, Furusawa Y, Itai S, Nakamura T, Yanaka M, Sano M, Harada H, Fukui M, Kaneko MK Abstract Podoplanin (PDPN) is known to be expressed in normal tissues, including lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPN have already been established; however, mAbs against pig PDPN (pPDPN) are lacking. In the present study, mice were immunized with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and hybridomas producing mAbs against pPDPN were identified by flo...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 28, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
In this study, R-phycoerythrin (R-PE) and Alexa Fluor 568 were separately conjugated to streptavidin. The efficiency of conjugations, R-PE-streptavidin and streptavidin-Alexa Fluor 568, were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and spectrophotometry, respectively. Herceptin, a humanized therapeutic antibody, was subsequently biotinylated. The reactivity of biotin-labeled Herceptin was examined by enzyme-linked immunosorbent assay. The photobleaching of R-PE-streptavidin and streptavidin-Alexa Fluor 568 were then compared in an immunofluorescent staining on a breast cancer cell line, BT-474...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-210: A Monoclonal Antibody Against Pig Podoplanin.
Authors: Furusawa Y, Yamada S, Itai S, Sano M, Nakamura T, Yanaka M, Fukui M, Harada H, Mizuno T, Sakai Y, Takasu M, Kaneko MK, Kato Y Abstract Podoplanin (PDPN) is a type I transmembrane glycoprotein that is expressed in normal tissues, including renal corpuscles and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPNs have already been established; however, antipig PDPN (pPDPN) mAbs have not. We therefore immunized mice with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and screened hybridomas, which are producing anti-pPDP...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 27, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of a Novel Monoclonal Antibody Against Chitinase-like Protein CHID1 Applicable for Immunohistochemistry on Formalin Fixed Paraffin-Embedded Sections.
In this study we describe the generation of a new hybridoma clone 3D4 producing anti-CHID1 antibodies. 3D4 mAb specifically binds human CHID1 and was successfully used in enzyme-linked immunosorbent assay, immunoblotting, immunofluorescence on paraformaldehyde-fixed cells, and in immunohistochemistry of paraffin-embedded tissue specimens. These results indicate that this new anti-CHID1 mAb 3D4 will be useful in the diagnosis of CHID1-related cancers and is a strong tool for both basic and clinical research on chitinase-like proteins. PMID: 30657411 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in I...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 20, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Diagnostic Tests for Detection of Helicobacter pylori Infection.
Authors: Mohammadian T, Ganji L Abstract Helicobacter pylori causes one of the most common infections in human populations. The role of this bacterium in chronic gastritis, gastric ulcer, gastric cancer, as well as extra-digestive diseases such as ischemic heart disease and chronic obstructive pulmonary diseases, is well known. Prevention and control of these diseases can occur by early diagnosis and eradication of H. pylori infection. At present, different methods have been established to detect H. pylori infection. The biopsy-based tests, which are known as invasive methods, such as rapid urease test and histolog...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 17, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Antidiacylglycerol Kinase α Monoclonal Antibody DaMab-2.
In this study, we characterized the binding epitope of DaMab-2 using Western blot and revealed that Cys246, Lys249, Pro252, and Cys253 of DGKα are important for DaMab-2 binding to the DGKα protein. Our findings can be applied for the production of more functional anti-DGKα mAbs. PMID: 30648914 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 17, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Anti-Horse Podoplanin Monoclonal Antibody PMab-219 is Useful for Detecting Lymphatic Endothelial Cells by Immunohistochemical Analysis.
Authors: Kato Y, Yamada S, Itai S, Kobayashi A, Konnai S, Kaneko MK Abstract Podoplanin (PDPN) is expressed in lymphatic endothelial cells, where it induces platelet aggregation through C-type lectin-like receptor-2 (CLEC-2). This protein has been characterized for a number of animal species using specific anti-PDPN monoclonal antibodies (mAbs). We recently established the mAb against horse PDPN (horPDPN) named PMab-219. Therefore, in this study, we investigated whether PMab-219 can detect lymphatic endothelial cells in horse tissues. Immunohistochemical analysis demonstrated that PMab-219 strongly stained lymphati...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 30, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Production and Characterization of Monoclonal and Polyclonal Antibodies Against Truncated Recombinant DKK-1 as a Candidate Biomarker.
Authors: PMID: 30592703 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 30, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Production and Characterization of Monoclonal and Polyclonal Antibodies Against Truncated Recombinant Dickkopf-1 as a Candidate Biomarker.
Authors: Malaei F, Rasaee MJ, Paknejad M, Latifi AM, Rahbarizadeh F Abstract Several studies have reported an increased serum level of Dickkopf (DKK-1) protein in a variety of cancers, including multiple myeloma, lung, colorectal, bone loss, and Alzheimer's disease. This protein has potential to be used as a biomarker for the diagnosis of some cancers, especially bone loss in multiple myeloma. In the present study, to measure the concentration level of DKK-1 protein, rabbit polyclonal antibody (pAb) and mouse monoclonal antibodies (mAbs) were produced against this protein. New Zealand white rabbits and BALB/c mice ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 30, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Crude Inactivated Influenza A Virus Adjuvated with a Bispecific Antibody Complex Targeting Chicken CD40 and AIV M2e Confers Protection Against Lethal HPAI Challenge in Chickens.
Authors: Vuong CN, Chou WK, Briggs W, Faulkner O, Wolfenden A, Jonas M, Kapczynski DR, Hargis BM, Bielke LR, Berghman LR Abstract In vivo targeting an immunogen to the CD40 receptor expressed on professional antigen-presenting cells (APCs) dramatically enhances speed, magnitude, and quality of the immune response. Our previous evaluation of this strategy in poultry was limited to immunogenicity studies using CD40-targeted synthetic peptides, which demonstrated significant antigen-specific serum IgG and tracheal IgA levels
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 30, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Galactose-Deficient IgA1-Specific Antibody Recognizes GalNAc-Modified Unique Epitope on Hinge Region of IgA1.
In this study, we aimed to analyze the epitope of KM55 mAb using synthesized peptides corresponding to the hinge region of IgA1 with GalNAc moiety on putative glycosylated Ser/Thr residues, which are Thr225, Thr228, Ser230, Ser232, and Thr236. Binding analysis to single GalNAc-modified hinge region peptide of IgA1 showed that Thr225 with GalNAc is required for recognition of KM55. PST(GalNAC)PP motif was required for KM55 mAb to recognize hinge region peptide of IgA1 which is shown by binding assay with deletion peptide. This result was confirmed by binding of KM55 mAb against peptide with GalNAc at Thr233, which resulted ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 21, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Immunohistochemical Detection of Sheep Podoplanin Using an Antibovine Podoplanin Monoclonal Antibody PMab-44.
In this study, we investigated possible cross-reactions between anti-PDPN mAbs and sheep PDPN. Type I alveolar cells from sheep lung were strongly detected by PMab-44 using immunohistochemical analyses. These results indicate that PMab-44 may be useful for the detection of sheep PDPN. PMID: 30570359 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 21, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Detection of Alpaca Podoplanin by Immunohistochemistry Using the Antibovine Podoplanin Monoclonal Antibody PMab-44.
In this study, we investigated the potential cross-reaction between established antibovine PDPN mAbs and alpaca PDPN. Using immunohistochemical analysis, type I alveolar cells of the alpaca lungs were detected by the antibovine PDPN mAb, PMab-44. These results indicate that PMab-44 may be useful for the detection of alpaca PDPN. PMID: 30570440 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 21, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Association of CDH1 and TERT Single-Nucleotide Polymorphisms with Susceptibility to Familial Breast Cancer Risk.
This study aimed to analyze the association of CDH1 and TERT single-nucleotide polymorphisms with susceptibility to familial BC (FBC) risk in the Iranian patients. One hundred five patients with FBC and 110 non-FBC (NFBC) were genotyped to elucidate the potential association between CDH1 rs5030625 polymorphism and TERT rs2736098 polymorphism by polymerase chain reaction-restriction fragment length polymorphism. Then, results were evaluated by electrophoresis and Epi Info(™) 2012 software. A significant association was found between CDH1 rs5030625 GAGA genotype and FBC risk. Compared with the control group, the FBC pa...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 15, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry.
In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG1, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN. PMID: 30457925 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 21, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of P38Bf, a Core-Fucose-Deficient Mouse-Canine Chimeric Antibody Against Dog Podoplanin.
In this study, we developed and produced several mouse-canine chimeric antibodies originating from PMab-38. A mouse-canine chimeric antibody of subclass A (P38A) and a mouse-canine chimeric antibody of subclass B (P38B) were transiently produced using ExpiCHO-S cells. Core-fucose-deficient P38B (P38Bf) was developed using FUT8 knockout ExpiCHO-S cells. We compared the binding affinities, antibody-dependent cellular cytotoxicity (ADCC), and complement-dependent cytotoxicity (CDC) of P38A, P38B, and P38Bf against Chinese hamster ovary (CHO)/dPDPN cells. Flow cytometry analysis showed that the KD of P38A, P38B, and P38Bf were...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 28, 2018 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research