Novel TLR2xTLR4 Bispecific Antibody Inhibits Bacterial Sepsis.
Authors: Sato M, Takeuchi S, Moriya R, Kito T, Soga S, Aoyama K, Suzuki J Abstract Toll-like receptors (TLRs) sense microbial infection through recognition of pathogen-associated molecular patterns. For example, TLR4 responds to the lipopolysaccharide of gram-negative bacteria, whereas TLR2 recognizes a broad range of microbial ligands. Both receptors are, therefore, compelling targets for treating sepsis. Here, we developed a TLR2xTLR4 tetravalent bispecific antibody designated ICU-1, which inhibits both receptors. The inhibitory activity of ICU-1 was comparable to that of the parental antibodies in neutralization...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 23, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody: Mouse Anti-Joining Chain of Human Immunoglobulin A.
Authors: PMID: 33351711 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 23, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Characterization of Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus Nonstructural Protein 13.
Authors: Chen J, Ding T, Liang W, Li T, Wu S, Xie W, Ding N, Tang Y, Ding Z Abstract Porcine epidemic diarrhea virus (PEDV) is an enteric swine coronavirus. Recent PEDV eruption in East Asian and North American countries made it notorious and caused huge economic losses to the porcine industry. Nonstructural protein 13 (nsp13) is a nucleic acid helicase/NTPase that plays a critical role in viral gene transcription and viral replication. To investigate the function of nsp13 in the context of PEDV infection, here, PEDV nsp13 gene was amplified and cloned into pET28a/pET30a/pGEX-6P-1 expression vectors. The recombinan...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 23, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody: 5A9, 5C7, and 5G7 Against Porcine Epidemic Diarrhea Virus nsp13.
Authors: PMID: 33351713 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 23, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Characterization of a Novel Anti-Rat TLR4/MD2 Antibody with Potent Neutralizing Activity In Vivo.
Authors: Sato M, Takeuchi S, Soga S, Aoyama K, Suzuki J Abstract Toll-like receptor 4 (TLR4) plays a critical role in the innate immune system and is involved in the pathogenesis of multiple diseases. Here, we report the antagonistic and ratized antibody, 52-1H4 e2 (e2), which completely inhibited lipopolysaccharide-induced interleukin-6 secretion in vitro. The average serum drug concentration was above 10 μg/mL for 28 days in rats injected with e2. The novel anti-rat TLR4/myeloid differentiation factor 2 antibody, e2, may be a useful tool for investigating the role of TLR4 in rat disease models. PMID...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Antibodies and Structure.
Authors: Kieber-Emmons T PMID: 33232641 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 26, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Perspective on Crystallographic Studies of Antibody Structures.
In this study, a brief overview is presented on how the crystal structures of antibody fragments guided therapeutic strategies emanating from our laboratories along with some historical perspective. PMID: 33156727 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

A Web-Based Platform on Coronavirus Disease-19 to Maintain Predicted Diagnostic, Drug, and Vaccine Candidates.
In this study, state-of-the-art techniques have been used for predicting the potential candidates for diagnostics and therapeutics. PMID: 33136473 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 3, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Highly Specific Mouse Anti-Joining Chain of Human Immunoglobulin A.
Authors: Ejemel M, Gawron MA, Schneider MI, Wallace A, Schiller ZA, Schneider R, Martin JC, Klempner MS, Wang Y, Cavacini LA Abstract Immunoglobulin A (IgA) antibodies are critical to mucosal protection, specifically dimeric IgA (dIgA) and secretory IgA (sIgA), which rely on the J chain to polymerize. There is an absence of monoclonal antibodies that can specifically bind to polymeric IgA without the need to denature the molecule. We generated a panel of highly specific mouse anti-J chain antibodies that react with both intact and denatured nonhuman primate dIgA and human dIgA and sIgA of both the IgA1 and IgA2 sub...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 31, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of Core-Fucose-Deficient Humanized and Chimeric Anti-Human Podoplanin Antibodies.
In this study, we first developed a humanized anti-human PDPN mAb, named as NZ-27. We further produced a core-fucose-deficient version of NZ-27, named as P1027 and a core-fucose-deficient version of NZ-12, named as NZ-12f. We investigated the binding affinity, antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and antitumor activity of P1027 and NZ-12f. We demonstrated that the binding affinities of P1027 and NZ-12f against LN319 (a human glioblastoma cell line) are 1.1 × 10-8 and 3.9 × 10-9 M, respectively. ADCC reporter assays demonstrated tha...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 23, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Against Human IgG (Anti-Human IgG).
Authors: Chu R PMID: 33085939 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 23, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

A Virus-Like Particle-Based Solid-Phase Competition Enzyme-Linked Immunosorbent Assay for Antibody Detection of Foot-and-Mouth Disease Virus Serotype O.
In this study, to minimize the risk of live virus involvement, the Escherichia coli original VLP of FMDV serotype O were used as the immunogen for monoclonal antibodies (Mabs) production and the capture antigen in the development of a solid-phase competition ELISA (SPCE). The samples with a percentage inhibition of>50% were considered positive in the SPCE assay. The concordance rate of the Mab-based SPCE compared with the LPBE for clinical serum samples test was 93.4%, and with a high agreement (kappa = 0.892) with LPBE in antibody duration monitoring. Results indicated that the VLP-based SPCE had high spe...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 23, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation of Rat Monoclonal Antibody for Cytokeratin 18 by Immunization of Three-Dimensional-Cultured Cancer Cells.
In this study, we produced rat monoclonal antibodies (mAbs) through immunization of the lysate from 3D-cultured DLD-1 cells to elucidate a characteristic feature of a tumor, and our results showed that mAb 2H7 recognized human CK18. Furthermore, we indicated that mAb 2H7 was useful for immunoblotting, immunoprecipitation, and immunofluorescence staining. Therefore, it may be useful as a diagnostic tool for evaluating malignancy. PMID: 33064594 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Need for a Global Network to Cope with Pandemics from Studies of the COVID Infection.
Authors: Kieber-Emmons T PMID: 32996828 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 2, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of Rabbit Monoclonal Antibodies for Quantitation of Therapeutic Human Antibodies in Preclinical Non-Human Primate Studies.
Authors: Chu R, Gerstein J, Wu H, Huang H, Lou Y, Palmer R Abstract During preclinical studies, there is a great need to develop monoclonal antibodies (mAbs) that are specific to human immunoglobulin (IgG), without binding to monkey IgG, to detect therapeutic human mAb in non-human primates. We took advantage of the latest rabbit B cell cloning technology to develop six unique rabbit anti-human IgG mAb clones for this purpose. These clones are capable of binding to both human IgG and Fab with high affinity without nonspecific binding to cynomolgus monkey IgG. These clones have been evaluated as a generic capture re...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 2, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibodies 6G6 and 7D2 Against Foot-and-Mouth Disease Virus Type O.
Authors: PMID: 33001741 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 2, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Efficacy and Safety of Anti-Interleukin-17A Monoclonal Antibody Secukinumab in Treatment of Ankylosing Spondylitis: A Meta-Analysis.
Authors: Katsevman GA, Mariscal G, Barrios C, Domenech-Fernández P, Ziembinski C, Bhatia S Abstract Different signaling pathways have been studied in ankylosing spondylitis. New treatment options such as secukinumab could have an important role inhibiting the release of proinflammatory cytokine IL-17. The aim of this study was to compare the efficacy and safety of secukinumab in ankylosing spondylitis. A systematic review was conducted using MEDLINE and EMBASE databases to identify randomized clinical trials (RCTs) that assess the role of secukinumab in ankylosing spondylitis. The variables were safety (tota...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 2, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Reflections on Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Authors: Steplewski Z PMID: 32822283 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 22, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Future of Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Authors: Kieber-Emmons T PMID: 32822284 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 22, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha.
This study establishes and evaluates a novel monoclonal antibody against canine CD8α (cCD8α). The antibody was produced by immunization of rats with cCD8α-expressing cells. After establishment and selection of hybridoma cells, the clone F3-B2 was established. The reactivity of F3-B2 was confirmed using cCD8α-overexpressing murine cells. Flow cytometric analysis also demonstrated that F3-B2 reacts with cCD8α naturally expressed in canine peripheral blood mononuclear cells and a canine T cell lymphoma cell line. The specimens of lymphoid tissue showed immunohistochemical staining for F3-B2. More...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 22, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

A Novel Hypothesis for Original Antigenic Sin in the Severe Disease of SARS-CoV-2 Infection.
Authors: Kohler H, Nara P Abstract In this hypothesis, we address the biological/immunological pathway leading to severe disease or death after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The underlying immune response is described with "original antigenic sin" (OAS) whereby previous infections influence the response to future virus encounters. We cite evidence for OAS-induced immunopathology in HIV-1 disease. We hypothesize that similar immune abnormalities can occur after infection with SARS-CoV-2. This hypothesis is supported by recent analysis of the antibodies in infe...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 13, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of Anti-Sheep Podoplanin Monoclonal Antibodies PMab-253 and PMab-260.
This study develops and characterizes anti-sheep PDPN (sPDPN) mAbs using Cell-Based Immunization and Screening (CBIS) method. A RAP14 tag was added to the N-terminus of sPDPN, and an anti-RAP14 tag mAb (PMab-2) was used to measure the expression level of sPDPN in flow cytometry and Western blots. We immunized mice with sPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/sPDPN) cells and screened mAbs against sPDPN using flow cytometry. Two of the mAbs, PMab-253 (immunoglobulin M [IgM], kappa) and PMab-260 (IgM, kappa), detected CHO/sPDPN cells specifically using flow cytometry and Western blots. Both PMab-253 and PMab...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 19, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of an Anti-CD20 Monoclonal Antibody (C20Mab-60) for Immunohistochemical Analyses.
This study aims to establish sensitive and specific anti-CD20 mAbs suitable for broad application, such as flow cytometry, Western blotting, and immunohistochemical analyses. Using the Cell-Based Immunization and Screening (CBIS) method, all procedures were performed by utilizing CD20-stable transfectants, and a clone, C20Mab-60 (IgG2a, kappa), was developed. In flow cytometry, C20Mab-60 detected overexpression of CD20 in LN229 cell and endogenous CD20 in BALL-1 (a human B cell leukemia cell line) but did not react with CD20-knockout BALL-1 (BINDS-24), indicating specificity for CD20. In Western blotting, C20Mab-60 detecte...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 16, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of DhMab-1: An Antidiacylglycerol Kinase Monoclonal Antibody.
Authors: Sano M, Kaneko MK, Kato Y Abstract Diacylglycerol kinase (DGK) η is classified as a type II DGK and catalyzes diacylglycerol phosphorylation to produce phosphatidic acid. DGKη has been reported to be highly expressed in the hippocampus and cerebellum. Although a DGKη-specific monoclonal antibody (mAb) is necessary to reveal the association between the expression of DGKη and diseases, an anti-DGKη mAb for immunohistochemistry has not been developed. Recently, we established a specific antihuman DGKη (hDGKη) mAb, DhMab-1 (mouse IgG2a, kappa). For epitope mapping of DhMab-1, here w...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

An Antihuman Epidermal Growth Factor Receptor 2 Monoclonal Antibody (H2Mab-19) Exerts Antitumor Activity in Glioblastoma Xenograft Models.
In this study, we investigated the antitumor activity of H2Mab-19 in an LN229 glioblastoma xenograft model. H2Mab-19 showed high binding affinity (KD: 1.1 × 10-8 M) against LN229 cells. Furthermore, H2Mab-19 significantly reduced tumor development in an LN229 xenograft. These results suggest that treatment with H2Mab-19 may be a useful therapy for patients with HER2-expressing glioblastomas. PMID: 32644843 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibodies BA5-C5, EG6-H6, AG5-B8: Antigen Is Aliphatic Di- and Polyisocyanate.
Authors: PMID: 32579494 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 26, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

An Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-19 Exerts Antitumor Activity in Mouse Colon Cancer Xenografts.
In this study, a novel anti-HER2 monoclonal antibody (mAb), H2Mab-19 (IgG2b, κ) was characterized for its anticancer activity in colon cancers. H2Mab-19 showed both antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity activities against Caco-2, a colon cancer cell line. Furthermore, H2Mab-19 significantly reduced tumor development in a Caco-2 xenograft model. These results suggest that treatment with H2Mab-19 may be a useful therapy for patients with HER2-expressing colon cancers. PMID: 32552424 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of the Anti-Diacylglycerol Kinase Monoclonal Antibody DhMab-4 for Immunohistochemical Analysis.
In this study, we established a specific anti-human DGKη (hDGKη) mAb, DhMab-4 (mouse IgG2b, kappa). DhMab-4 strongly stained Purkinje cells of human cerebellum in immunohistochemistry analysis. For epitope mapping of DhMab-4, we produced deletion or point mutants of hDGKη and performed western blotting to determine the binding epitope of DhMab-4. DhMab-4 reacted with dN745 mutant but not with dN750 mutant, indicating that the N-terminus of the DhMab-4 epitope is located between amino acids 745 and 750. More detailed analysis using point mutants demonstrated that five mutants, that is, D747A, P748A, F749A, G750A...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 5, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

H2Mab-19 Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody Therapy Exerts Antitumor Activity in Pancreatic Cancer Xenograft Models.
In this study, we investigated the antitumor activities of H2Mab-19 in pancreatic cancer xenograft models. We selected MIA PaCa-2, a pancreatic cancer cell line which expresses HER2. H2Mab-19 showed high binding affinity (KD: 1.2 × 10-8 M) against MIA PaCa-2 cells. Furthermore, H2Mab-19 significantly reduced tumor development in a MIA PaCa-2 xenograft model. These results suggest that treatment with H2Mab-19 may be a useful therapy for patients with HER2-expressing pancreatic cancers. PMID: 32423281 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Thr80 of Sheep Podoplanin Is a Critical Epitope of the Antisheep Podoplanin Monoclonal Antibody: PMab-256.
Authors: Kato Y, Sano M, Asano T, Sayama Y, Kaneko MK Abstract An antisheep podoplanin (sPDPN) monoclonal antibody (mAb), PMab-256, has recently been established. PMab-256 shows positive immunostaining for lymphatic endothelial cells, lung type I alveolar cells, and kidney podocytes. PDPN possesses three platelet-aggregation-stimulating (PLAG) domains, PLAG1, PLAG2, and PLAG3, and a PLAG-like domain (PLD). The binding epitope of many anti-PDPN mAbs is located in PLAG domains or PLD. The purpose of this study is to determine the binding epitope of PMab-256. Analysis of sPDPN deletion mutants revealed that the N-term...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Detection of Lion Podoplanin Using the Antitiger Podoplanin Monoclonal Antibody PMab-231.
In this study, possible cross-reaction between available anti-PDPN mAbs and lioPDPN was examined. Immunohistochemical analysis showed that antitiger PDPN mAb PMab-231 (IgG2a, kappa) reacted with type I alveolar cells from lion lung, indicating that PMab-231 is useful for the detection of lioPDPN. PMID: 32401677 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 14, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of an Anti-Sheep Podoplanin Monoclonal Antibody PMab-256 for Immunohistochemical Analysis of Lymphatic Endothelial Cells.
In this study, we used the Cell-Based Immunization and Screening method for the development of anti-sPDPN mAbs. RAP14 tag was added to N-terminus of sPDPN, and anti-RAP14 tag mAb (PMab-2) was used to detect the expression level of sPDPN in flow cytometry and western blot. We immunized mice with sPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/sPDPN) cells and screened mAbs against sPDPN using flow cytometry. One of the mAbs, PMab-256 (IgG1, kappa), specifically detected CHO/sPDPN cells by flow cytometry and western blot. Furthermore, PMab-256 stained type I alveolar cells of lung, renal glomerulus and Bowman's caps...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 1, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA Immunization.
In this study, we report the generation and characterization of a monoclonal antibody (mAb) against the extracellular domain of hCD63 using DNA immunization. This mAb, clone 1C8-2B11, exhibits high performance for use in immunofluorescence and flow cytometry, and it has 10-fold higher affinity than the control antibody that is commercially available. mAb 1C8-2B11 has great potential to be a tool for research and clinical diagnosis. PMID: 32311306 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Molecular Characterization and Experimental Utility of Monoclonal Antibodies with Specificity for Aliphatic Di- and Polyisocyanates.
Authors: Wisnewski AV, Liu J Abstract Aliphatic di- and polyisocyanates are crucial chemical ingredients in many industrial processes and are a well-recognized cause of occupational asthma. Serologic detection of "chemical epitopes" in biological samples could serve as an exposure surveillance approach toward disease prevention, and thus we sought to generate aliphatic isocyanate-specific monoclonal antibodies (mAbs). Three hybridomas were generated from Balb/c mice immunized with a commercial product containing a combination of uretdione, homopolymer, and monomeric forms of hexamethylene diisocyanate (HD...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 19, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of Novel Mouse Monoclonal Antibodies Against Human CD19.
In this study, we first employed the Cell-Based Immunization and Screening (CBIS) method to produce anti-CD19 monoclonal antibodies using CD19-overexpressing cells for both immunization and screening. One established clone-C19Mab-1-proved to be useful in flow cytometry assays against lymphoma cell lines, such as BALL-1, P30/OHK, and Raji. Second, the extracellular domain of CD19 was immunized into mice, and enzyme-linked immunosorbent assays were performed for the first screening. One established clone-C19Mab-3-was determined to be useful for Western blotting and immunohistochemical analysis. Due to their complementary uti...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 10, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of PMab-241, a Lymphatic Endothelial Cell-Specific Anti-Bear Podoplanin Monoclonal Antibody.
Authors: Sayama Y, Sano M, Asano T, Furusawa Y, Takei J, Nakamura T, Yanaka M, Okamoto S, Handa S, Komatsu Y, Nakamura Y, Yanagawa M, Kaneko MK, Kato Y Abstract Anti-bear podoplanin (bPDPN) monoclonal antibodies (mAbs), including PMab-247 and PMab-241, have been previously established. Although PMab-247 has shown positive immunostaining for lymphatic endothelial cells (LECs), type I alveolar cells of the lung, and podocytes of the kidney, PMab-241 stains LECs but does not react with lung type I alveolar cells. PDPN possesses three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) and the PLA...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 4, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Peptide-Based Monoclonal Antibody Production Against SAG1 (P30) Protein of Toxoplasma gondii.
Authors: Naghi Vishteh M, Javadi Mamaghani A, Rashidi S, Seyyed Tabaei SJ, Jeddi-Tehrani M Abstract Toxoplasma gondii is an intracellular protozoan parasite that can infect a wide range of warm-blooded animals. Humans as an intermediate host are infected by ingesting infectious oocytes or tissue cysts, or passing through the placenta in pregnant women. The aim of this study is producing monoclonal antibodies against a synthetic peptide from (surface antigen 1 [SAG1] or P30) protein of T. gondii. A synthetic peptide from SAG1 (P30) protein was conjugated to Keyhole Limpet Hemocyanin (KLH (and then used for immunizat...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 30, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Expression Pattern of Plexin Domain Containing 2 in Human Hepatocellular Carcinoma.
In this study, we investigated the expression pattern of PLXDC2 in human hepatocellular carcinoma (HCC) tissues using a highly specific anti-PLXDC2 rabbit monoclonal antibody, which was recently developed. PLXDC2 was expressed in human HCC tissues including HCC cells, tumor vascular endothelial cells, and some infiltrating cells. The developed anti-PLXDC2 antibody allowed for highly specific and low background staining. Based on these current findings of PLXDC2 expression in human HCC tissues, the window may now be open to explore the role of PLXDC2 in human HCC. PMID: 32202949 [PubMed - as supplied by publisher] (Sour...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 25, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Safety of Romosozumab in Osteoporotic Men and Postmenopausal Woman: A Meta-Analysis and Systematic Review.
Authors: Mariscal G, Nuñez JH, Bhatia S, Barrios C, Domenech-Fernández P Abstract Sclerostin is a protein synthesized mainly by osteocytes whose function is to inhibit bone formation. A recent monoclonal antibody, Romosozumab, is able to block sclerostin. The aim of this meta-analysis is to compare the safety of Romosozumab with placebo and alendronate. Five randomized controlled trials that described the safety of Romosozumab in healthy men and postmenopausal women were analyzed. The measures to be compared were the number of adverse events and the number of serious adverse events. Specific results i...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 21, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Antibody-Drug Conjugates Using Mouse-Canine Chimeric Anti-Dog Podoplanin Antibody Exerts Antitumor Activity in a Mouse Xenograft Model.
In this study, we developed an ADC, P38B-DM1, using the mouse-canine chimeric anti-dPDPN antibody, P38B as the antibody, a peptide linker, and emtansine as the payload using the chemical conjugation by affinity peptide (CCAP) method. We investigated its cytotoxicity against dPDPN-overexpressed Chinese hamster ovary (CHO/dPDPN) cells in vitro and its antitumor activity using a mouse xenograft model of CHO/dPDPN cells. P38B-DM1 showed cytotoxicity to CHO/dPDPN cells in a dose-dependent manner in vitro. Furthermore, P38B-DM1 exhibited higher antitumor activity than P38B in the mouse xenograft model. These results suggest that...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 18, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of a Monoclonal Antibody That Recognizes Cysteine-Rich Domain 1 of Human CD271.
Authors: Morita S, Mochizuki M, Shibuya-Takahashi R, Nakamura-Shima M, Yamazaki T, Imai T, Asada Y, Matsuura K, Kawamura S, Yamaguchi K, Yasuda J, Sugamura K, Katori Y, Satoh K, Tamai K Abstract CD271 is a common receptor for all neurotrophins that is localized to neurons, endothelial cells, and the basal layer of the epithelium in normal tissue. Recently, we and others reported that CD271 plays essential roles in the development of squamous cell carcinoma, especially in tumor-initiating cells. Since little is known about how CD271 regulates cancer cell initiation and proliferation, antibodies that recognize differ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Against Stromal Cell-Derived Factor 2.
Authors: PMID: 32069134 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody MAb 44A11 Against Sodium Nifurstyrenate.
Authors: PMID: 32069135 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 20, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Production and Characterization of Monoclonal Antibodies Against Gp Protein of Ebola Virus.
Authors: Yu M, Ye J, Cao S, Liu X, Chen Z Abstract The DNA fragment encoding predicted main antigenic region, aa 1-300 on Gp protein of Ebola virus (EBOV) was cloned into the vector pGEX-KG. The recombinant GST-tagged Gp-300 was expressed in Escherichia coli BL21 (DE3) by induction with 1 mM isopropyl-1-thio-b-d-galactoside and purified by dialysis. Four monoclonal antibodies (mAbs) named 1C4, 2A3, 2G7, and 2H9 against Gp protein were generated by fusing mouse myeloma cell line SP2/0 with spleen lymphocytes from Gp-300 protein-immunized mice. The activity of the mAbs was then characterized by enzyme-linked i...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 2, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Analysis of an Anti-Whale Podoplanin Monoclonal Antibody, PMab-237, Using Flow Cytometry.
In this study, deletion mutants and point mutants of wPDPN with N-terminal RIEDL tag were produced to analyze the PMab-237 epitope using flow cytometry. The analysis of deletion mutants showed that the N-terminus of the PMab-237 epitope exists between the 80th amino acid (AA) and the 85th AA of wPDPN. In addition, the analysis of point mutants demonstrated that the critical epitope of PMab-237 includes Leu82 and Thr84 of wPDPN, indicating that the PMab-237 epitope is located in the PLD of wPDPN. PMID: 31934820 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 15, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2.
In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells. PMID: 31916900 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 11, 2020 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Characterization of a Novel Bispecific Antibody That Activates T Cells In Vitro and Slows Tumor Growth In Vivo.
Authors: Chornoguz O, Leettola CN, Leander K, Brosnan K, Emmell E, Chiu ML, Santulli-Marotto S Abstract Although CD3 T cell redirecting antibodies have been successfully utilized for the treatment of hematological malignancies (blinatumomab), the T cell signaling pathways induced by these molecules are incompletely understood. To gain insight into the mechanism of action for T cell redirection antibodies, we created a novel murine CD3xEpCAM bispecific antibody that incorporates a silent Fc to dissect function and signaling of murine CD8 OT1 T cells upon stimulation. T cell-mediated cytotoxicity, cytokine secretion,...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 12, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Seroepidemiology Study of Helicobacter pylori Infection and Its Effect on Cardiovascular Diseases in Karaj, Iran.
Authors: Afsharpooyan S, Mohammadian T Abstract Helicobacter pylori infection occurs throughout the world between countries and races. A seroprevalence study of H. pylori infection in the occurrence of atherosclerosis (ASc) has been considered to be the main cause of cardiovascular diseases (CVDs) in part of Iran. From 600 volunteers, 97 serum samples were selected and tested for the presence of H. pylori infection. Positive and negative H. pylori infection samples were then selected for Chlamydia pneumonia and cytomegalovirus infection. Their serum levels for high-sensitive C-reactive protein (hs-CRP) and IL-6 tit...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 21, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein.
This study shows that, due to the advantages such as multi-epitope-binding ability, camelid pAbs are potent to efficiently apply for molecular detection of CTLA-4 receptors in fundamental antibody-based researches such as IHC. PMID: 31718460 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-241 Specifically Detects Bear Podoplanin of Lymphatic Endothelial Cells in the Lung of Brown Bear.
In this study, we showed that a novel anti-bPDPN mAb, PMab-241 stained only lymphatic endothelial cells, not type I alveolar cells of the lung in immunohistochemical analyses. These findings suggest that PMab-241 could be useful for staining lymphatic endothelial cells specifically in the bear lung tissues. PMID: 31721640 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 15, 2019 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research