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Cx 4 Mab-1: A Novel Anti-Mouse CXCR4 Monoclonal Antibody for Flow Cytometry
This study established a novel anti-mouse CXCR4 (mCXCR4) mAb using the Cell-Based Immunization and Screening method. Flow cytometric analysis showed that an anti-mCXCR4 mAb, Cx4Mab-1 (rat IgG2a, kappa), recognized mCXCR4-overexpressed Chinese hamster ovary-K1 (CHO/mCXCR4) cells and endogenously mCXCR4-expressing mouse myeloma P3X63Ag8U.1 (P3U1) cells. Furthermore, Cx4Mab-1 did not recognize mCXCR4-knockout P3U1 cells. The dissociation constants of Cx4Mab-1 for CHO/mCXCR4 and P3U1 were determined as 6.4 × 10-9 M and 2.3 × 10-9 M, respectively, indicating that Cx4Mab-1 possesses a high affinity to both endogenous and exoge...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 21, 2023 Category: Microbiology Authors: Tsunenori Ouchida Hiroyuki Suzuki Tomohiro Tanaka Mika K Kaneko Yukinari Kato Source Type: research

A Rat Anti-Mouse CD39 Monoclonal Antibody for Flow Cytometry
In this study, we developed novel mAbs for mouse CD39 (mCD39) using the Cell-Based Immunization and Screening (CBIS) method. One of the established anti-mCD39 mAbs, C39Mab-2 (rat IgG2a, lambda), reacted with mCD39-overexpressed Chinese hamster ovary-K1 (CHO/mCD39) and an endogenously mCD39-expressed cell line (SN36) by flow cytometry. The kinetic analysis using flow cytometry indicated that the dissociation constant (KD) values of C39Mab-2 for CHO/mCD39 and SN36 were 5.5 × 10-9 M and 4.9 × 10-9 M, respectively. These results indicated that C39Mab-2 is useful for the detection of mCD39 in flow cytometry.PMID:38126892 | DO...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 21, 2023 Category: Microbiology Authors: Hiroyuki Suzuki Tomohiro Tanaka Yuma Kudo Mayuki Tawara Aoi Hirayama Mika K Kaneko Yukinari Kato Source Type: research

Prevention of Collagen-Induced Arthritis by an Anti-Glycan Monoclonal Antibody Reactive with 6-Sulfo Sialyl Lewis x in DBA/1 Mice
Monoclon Antib Immunodiagn Immunother. 2023 Dec 8. doi: 10.1089/mab.2023.0019. Online ahead of print.ABSTRACTRheumatoid arthritis (RA) is an autoimmune disease characterized by synovial tissue inflammation, substantially impacting the quality of life of patients. The interaction between L-selectin and its glycoprotein ligands modified with 6-sulfo sialyl Lewis x (6-sulfo sLex) is known to mediate lymphocyte homing to initiate immune responses. Thus, this process could be a potential therapeutic target for RA. Herein, we explored the preventive effects of an anti-6-sulfo sLex monoclonal antibody (mAb), SF1, on collagen-indu...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 8, 2023 Category: Microbiology Authors: Zihong Wei Hiroto Kawashima Source Type: research

Development of Monoclonal Antibodies Specific to Either CD300A R111 or CD300A Q111 or Both
In this study, we successfully generated monoclonal antibodies (mAbs) specific to either CD300AR111 or CD300AQ111 or both. These mAbs are useful for the analysis of CD300A genotype by flow cytometry and the development of an antibody drug for the treatment of various diseases.PMID:37902989 | DOI:10.1089/mab.2023.0017 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 30, 2023 Category: Microbiology Authors: Hitoshi Koizumi Chigusa Nakahashi-Oda Wenxin Lyu Yumi Yamashita-Kanemaru Keiji Tabuchi Kazuko Shibuya Akira Shibuya Source Type: research

Development and Characterization of a Novel Neuropilin-2 Antibody for Immunohistochemical Staining of Cancer and Sarcoidosis Tissue Samples
Monoclon Antib Immunodiagn Immunother. 2023 Oct;42(5):157-165. doi: 10.1089/mab.2023.0007.ABSTRACTNeuropilin-2 (NRP2) is a cell surface receptor that plays key roles in lymphangiogenesis, but also in pathophysiological conditions such as cancer and inflammation. NRP2 targeting by efzofitimod, a novel immunomodulatory molecule, is currently being tested for the treatment of pulmonary sarcoidosis. To date, no anti-NRP2 antibodies are available for companion diagnostics. Here we describe the development and characterization of a novel NRP2 antibody. Using a variety of research techniques, that is, enzyme-linked immunoassay, W...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 30, 2023 Category: Microbiology Authors: Sarah F örster Yeeting E Chong David Siefker Yvonne Becker Ruizhi Bao Erik Escobedo Yang Qing Kaitlyn Rauch Luke Burman Christoph Burkart Philipp Kainz Andrea Cubitt Michael Muders Leslie A Nangle Source Type: research