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Establishment of a Novel Anti-Human CCR6 Monoclonal Antibody C < sub > 6 < /sub > Mab-19 with the High Binding Affinity in Flow Cytometry
In this study, we developed novel monoclonal antibodies (mAbs) against human CCR6 (hCCR6) using the peptide immunization method, which are applicable to flow cytometry and immunohistochemistry. The established anti-hCCR6 mAb, clone C6Mab-19 (mouse IgG1, kappa), reacted with hCCR6-overexpressed Chinese hamster ovary-K1 (CHO/hCCR6), human liver carcinoma (HepG2), and human differentiated hepatoma (HuH-7) cells in flow cytometry. The dissociation constant (KD) of C6Mab-19 was determined as 3.0 × 10-10 M for CHO/hCCR6, 6.9 × 10-10 M for HepG2, and 1.8 × 10-10 M for HuH-7. Thus, C6Mab-19 could bind to exogenously and endogen...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 10, 2023 Category: Microbiology Authors: Tomohiro Tanaka Kaishi Kitamura Hiroyuki Suzuki Mika K Kaneko Yukinari Kato Source Type: research