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Monoclonal Antibody Rat 2F11 and Rabbit A3 Against Anti-H2b3b
Monoclon Antib Immunodiagn Immunother. 2024 Apr 2. doi: 10.1089/mab.2024.0005. Online ahead of print.NO ABSTRACTPMID:38563773 | DOI:10.1089/mab.2024.0005 (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 2, 2024 Category: Microbiology Authors: Saki Egashira Taro Tachibana Mako Nakamura Yasuyuki Ohkawa Akihito Harada Source Type: research

Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H 2 Mab-250/H 2 CasMab-2 for Breast Cancers
In this study, we established a novel cancer-specific anti-HER2 monoclonal antibody, named H2Mab-250/H2CasMab-2 (IgG1, kappa). H2Mab-250 reacted with HER2-positive breast cancer BT-474 and SK-BR-3 cells. Importantly, H2Mab-250 did not react with nontransformed normal epithelial cells (HaCaT and MCF 10A) and immortalized normal epithelial cells in flow cytometry. In contrast, most anti-HER2 mAbs, such as H2Mab-119 and trastuzumab reacted with both cancer and normal epithelial cells. Immunohistochemical analysis demonstrated that H2Mab-250 possesses much higher reactivity to the HER2-positive breast cancer tissues compared w...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 2, 2024 Category: Microbiology Authors: Mika K Kaneko Hiroyuki Suzuki Yukinari Kato Source Type: research

Establishment of a Novel Anti-Mouse CCR1 Monoclonal Antibody C 1 Mab-6
Monoclon Antib Immunodiagn Immunother. 2024 Mar 21. doi: 10.1089/mab.2023.0032. Online ahead of print.ABSTRACTC-C motif chemokine receptor 1 (CCR1/CD191) is a member of G-protein-coupled receptors and is expressed on myeloid cells, such as neutrophils and macrophages. Because the CCR1 signaling promotes tumor expansion in the tumor microenvironment (TME), the modification of TME is an effective strategy for cancer therapy. Although CCR1 is an attractive target for solid tumors and hematological malignancies, therapeutic agents for CCR1 have not been approved. Here, we established a novel anti-mouse CCR1 (mCCR1) monoclonal ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 21, 2024 Category: Microbiology Authors: Tsunenori Ouchida Yu Isoda Takuro Nakamura Miyuki Yanaka Tomohiro Tanaka Saori Handa Mika K Kaneko Hiroyuki Suzuki Yukinari Kato Source Type: research

Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C 44 Mab-108) Using Enzyme-Linked Immunosorbent Assay
In this study, we determined the critical epitope of C44Mab-108 by enzyme-linked immunosorbent assay (ELISA). We used the alanine (or glycine)-substituted peptides of the CD44v4-encoded region (amino acids 271-290 of human CD44v3-10) and found that C44Mab-108 did not recognize the alanine-substituted peptides of D280A and W281A. Furthermore, these peptides could not inhibit the recognition of C44Mab-108 in flow cytometry and immunohistochemistry. The results indicate that the critical binding epitope of C44Mab-108 includes Asp280 and Trp281 of CD44v3-10.PMID:38507669 | DOI:10.1089/mab.2023.0022 (Source: Monoclonal Antibodi...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 20, 2024 Category: Microbiology Authors: Hiroyuki Suzuki Mayuki Tawara Aoi Hirayama Nohara Goto Tomohiro Tanaka Mika K Kaneko Yukinari Kato Source Type: research

Cx 3 Mab-4: A Novel Anti-Mouse CXCR3 Monoclonal Antibody for Flow Cytometry
In this study, we established a novel anti-mouse CXCR3 (mCXCR3) monoclonal antibody, Cx3Mab-4 (rat IgG1, kappa), using the Cell-Based Immunization and Screening method. Flow cytometric analysis demonstrated that Cx3Mab-4 bound to mCXCR3-overexpressed Chinese hamster ovary-K1 (CHO/mCXCR3) cells, but did not react to parental CHO-K1 cells. The dissociation constant of Cx3Mab-4 was determined as 1.3 × 10-9 M, indicating that Cx3Mab-4 possesses a high affinity to mCXCR3-expressing cells. Cx3Mab-4 could be useful for targeting CXCR3-expressing cells in preclinical mouse models.PMID:38507670 | DOI:10.1089/mab.2023.0024 (Source:...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 20, 2024 Category: Microbiology Authors: Tsunenori Ouchida Yu Isoda Tomohiro Tanaka Mika K Kaneko Hiroyuki Suzuki Yukinari Kato Source Type: research

Epitope Mapping of an Anti-Mouse CD39 Monoclonal Antibody Using PA Scanning and RIEDL Scanning
In this study, we determined the critical epitope of C39Mab-1 using flow cytometry. We performed the PA tag (12 amino acids [aa])-substituted analysis (named PA scanning) and RIEDL tag (5 aa)-substituted analysis (named RIEDL scanning) to determine the critical epitope of C39Mab-1 using flow cytometry. By the combination of PA scanning and RIEDL scanning, we identified the conformational epitope, spanning three segments of 275-279, 282-291, and 306-323 aa of mCD39. These analyses would contribute to the identification of the conformational epitope of membrane proteins.PMID:38507671 | DOI:10.1089/mab.2023.0029 (Source: Mono...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 20, 2024 Category: Microbiology Authors: Yuki Okada Hiroyuki Suzuki Tomohiro Tanaka Mika K Kaneko Yukinari Kato Source Type: research