Prokaryotic Expression of Hepatitis C Virus-NS3 Protein and Preparation of a Monoclonal Antibody.
Authors: Xi Y, Zhang Y, Fang J, Whittaker K, Luo S, Huang RP Abstract Hepatitis C virus (HCV) is a significant health threat that has been extensively investigated worldwide. Improving the sensitivity and specificity of laboratory tests for screening and early diagnosis of HCV in a relevant population is an effective measure to control the spread of HCV. To build a more reliable diagnostic method for HCV, we expressed gene fragments of HCV-NS3 linked to a carrier, pET28a, and then transformed this vector into Escherichia. coli. The produced recombinant NS3 protein with a molecular weight of 38 kDa, which was...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Preparation of a Monoclonal Antibody Against gD Protein of Bovine Herpesvirus I.
Authors: Ran X, Yang Z, Wen X, Bi Y, Ni H Abstract The two DNA fragments encoding predicted main antigenic regions, aa 20-160 and aa 257-344 on gD protein of bovine herpesvirus-1 (BoHV-1) were tandem-cloned into the vector pET28a. The recombinant His-tagged Δ gD1-Δ gD2 was expressed in Escherichia coli BL21(DE3)pLysS by induction with 0.5 mM isopropyl-1-thio-β-D-galactoside (IPTG) and Western blot analysis demonstrated that the recombinant His-tagged Δ gD1-Δ gD2 reacted with the positive serum against BoHV-1. A monoclonal antibody (MAb), designated as 2B6, was prepared by fusion ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-48 Recognizes Dog Podoplanin of Lymphatic Endothelial Cells.
In this study, we newly developed PMab-48 against dog podoplanin. Immunohistochemical analysis revealed that PMab-48 reacts not only with canine squamous cell carcinoma cells but also with LECs of the normal colon. Therefore, PMab-48 may be useful in investigating the function of dog podoplanin in LECs. PMID: 29256716 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 21, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Production of Monoclonal Antibody That Recognizes Zika Virus and Other Flaviviruses in Serum-Free Conditions.
This study aimed to cultivate, in serum-free conditions, the 4G2 hybridoma that produces an mAb, which recognizes a shared epitope from the Flavivirus genus. We compared 4G2 hybridoma growth and biochemical profiles between cells cultivated in batch mode over 10 days in roller bottles containing Dulbecco's modified Eagle's medium high glucose containing 10% fetal bovine serum medium or hybridomas directly adapted to Ex-Cell serum-free medium. Cellular parameters such as specific growth rate (μ), maximum cell concentration, specific l-lactate, and glucose and IgG rates were evaluated. Thereafter, we also compared total m...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 8, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of H2Mab-119, an Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody, Against Pancreatic Cancer.
Authors: Yamada S, Itai S, Nakamura T, Chang YW, Harada H, Suzuki H, Kaneko MK, Kato Y Abstract Human epidermal growth factor receptor 2 (HER2) is overexpressed in breast cancer and is associated with poor clinical outcomes. In addition, HER2 expression has been reported in other cancers, such as gastric, colorectal, lung, and pancreatic cancers. An anti-HER2 humanized antibody, trastuzumab, leads to significant survival benefits in patients with HER2-overexpressing breast cancers and gastric cancers. Herein, we established a novel anti-HER2 monoclonal antibody (mAb), H2Mab-119 (IgG1, kappa), and characterized its ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 28, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Epitope Mapping of Monoclonal Antibody PMab-38 Against Dog Podoplanin.
In this study, flow cytometry was utilized to investigate the epitope of PMab-38, which was determined using a series of deletion or point mutants of dPDPN. The results revealed that the critical epitope of PMab-38 is Tyr67 and Glu68 of dPDPN. PMID: 29161183 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Expression of Cat Podoplanin in Feline Squamous Cell Carcinomas.
In this study, we investigated podoplanin expression in 40 feline squamous cell carcinomas (14 cases of mouth floor, 13 of skin, 9 of ear, and 4 of tongue) by immunohistochemical analysis using an anti-cat podoplanin mAb PMab-52, which we recently developed by cell-based immunization and screening (CBIS) method. Of the total 40 cases, 38 (95%) showed positive staining for PMab-52. In particular, 12 cases (30%) showed a strong membrane-staining pattern of squamous cell carcinoma cells. PMab-52 can be useful for antibody therapy against feline podoplanin-expressing squamous cell carcinomas. PMID: 29090969 [PubMed - as su...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 2, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of EMab-134, a Sensitive and Specific Anti-Epidermal Growth Factor Receptor Monoclonal Antibody for Detecting Squamous Cell Carcinoma Cells of the Oral Cavity.
In this study, we established novel anti-EGFR monoclonal antibodies (mAbs) and characterized their efficacy in flow cytometry, Western blot, and immunohistochemical analyses. We immunized mice with a combination of the extracellular domain of EGFR and EGFR-overexpressing LN229 glioblastoma cells (LN229/EGFR) and performed the first screening using enzyme-linked immunosorbent assay. Next, we selected mAbs using flow cytometry. Among 156 established clones, two mAbs, EMab-51 (IgG1, kappa) and EMab-134 (IgG1, kappa), reacted with EGFR in Western blot analysis; EMab-134 showed a much higher sensitivity compared with EMab-51. W...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - November 2, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Association of Mannose-Binding Lectin rs1800450 and Tumor Necrotic Factor- α rs1800620 Polymorphism with Helicobacter pylori in Type II Diabetes Mellitus.
In this study, we investigate the prevalence rate of H. pylori infection and its relationship to MBL rs1800450 and TNF-α rs1800620 polymorphism in T2DM. In this case-control study, 174 patients with type II diabetes and 185 healthy controls were studied. Also, demographics, physical, and biochemical parameters were performed in all patients. The DNA extracted from blood specimens was amplified by H. pylori cagA-specific primers. The MBL rs1800450 and TNF-α rs1800620 genotyping were detected by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The results show that H. pylori cagA pos...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Anti-hLCN6 Monoclonal Antibody.
Authors: PMID: 29053426 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Dendrimers, Carotenoids, and Monoclonal Antibodies.
Authors: Bashmakov YK, Petyaev IM Abstract Dendrimers are unimolecular architectural nano- or microparticle entities that can accommodate various nutraceuticals and pharmaceuticals between their branches (dendrons) and provide targeted delivery of biomimetics into different tissues upon addition of functionalized groups to the dendrimer's surface. Covalent binding, hydrogen bonds, and electrostatic interactions between dendrimer-composing molecules are known to form and stabilize dendrimer structure. Carotenoids have recently been shown to form dendrimer-like structures and promote targeted delivery of "cargo&...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 12, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Target-Specific Delivery of an Antibody That Blocks the Formation of Collagen Deposits in Skin and Lung.
In this study, we analyzed target specificity, which is a main parameter that defines the safe use of all antibody-based therapies in humans. We hypothesized that, regardless of the route of administration, our antibody would preferentially bind to free collagen molecules synthesized at the sites of fibrosis and have minimal off-target interactions when applied in various tissues. To test this hypothesis, we used two experimental models of organ fibrosis: (1) a keloid model, in which antibody constructs were directly implanted under the skin of nude mice and (2) an experimental model of pulmonary fibrosis, in which our ant...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - October 5, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry.
Authors: Itai S, Fujii Y, Nakamura T, Chang YW, Yanaka M, Saidoh N, Handa S, Suzuki H, Harada H, Yamada S, Kaneko MK, Kato Y Abstract CD133, also known as prominin-1, was first described as a cell surface marker on early progenitor and hematopoietic stem cells. It is a five-domain transmembrane protein composed of an N-terminal extracellular tail, two small cytoplasmic loops, two large extracellular loops containing seven potential glycosylation sites, and a short C-terminal intracellular tail. CD133 has been used as a marker to identify cancer stem cells derived from primary solid tumors and as a prognostic marker...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - September 15, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of EMab-51, a Sensitive and Specific Anti-Epidermal Growth Factor Receptor Monoclonal Antibody in Flow Cytometry, Western Blot, and Immunohistochemistry.
In this study, we developed novel anti-EGFR monoclonal antibodies (mAbs) and characterized their efficacy in flow cytometry, Western blot, and immunohistochemical analyses. First, we expressed the full-length or ectodomain of EGFR in LN229 glioblastoma cells and then immunized mice with LN229/EGFR or ectodomain of EGFR, and performed the first screening using enzyme-linked immunosorbent assays. Subsequently, we selected mAbs according to their efficacy in flow cytometry (second screening), Western blot (third screening), and immunohistochemical (fourth screening) analyses. Among 100 mAbs, only one clone EMab-51 (IgG1, kapp...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - September 12, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Immunohistochemical Analysis Using Antipodocalyxin Monoclonal Antibody PcMab-47 Demonstrates Podocalyxin Expression in Oral Squamous Cell Carcinomas.
In this study, we performed immunohistochemical analysis against oral cancers using PcMab-47. PcMab-47-stained oral squamous cell carcinoma cells in a cytoplasmic pattern and detected 26/38 (68.4%) of oral squamous cell carcinoma cells on tissue microarrays. These results indicate that PcMab-47 is useful in detecting podocalyxin of oral cancers for immunohistochemical analysis. PMID: 28873000 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - September 6, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of a Monoclonal Antibody Against chGILZ.
Authors: He Z, Wang Y Abstract The chicken glucocorticoid-induced leucine zipper (chGILZ) participates in the inflammation of avian immunosuppressive diseases. We aimed to establish a monoclonal antibody (MAb) against chGILZ and to investigate its distribution in clinical diagnosis. A gene cloned from chicken embryo fibroblast (CEF) cell was inserted into the expression vector pET-28a and pGEX-6p-1. The recombinant expression vectors were transformed into Escherichia coli BL21 (DE3). Then the recombinant proteins His-chGILZ and GST-chGILZ were successfully expressed and purified. The hybridomas were developed by fu...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 16, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Chicken HMGB1 Monoclonal Antibody.
Authors: PMID: 28806152 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 16, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Expression, Purification, and Refolding of Human Lipocalin 6 and Production of a Monoclonal Antibody Against This Protein.
In this study, we report the prokaryotic expression, purification, and refolding of recombinant hLCN6. Purified hLCN6 protein was used to generate monoclonal antibody (mAb) against this protein using conventional hybridoma techniques. The sensitivity and specificity of the anti-hLCN6 mAb were determined based on their activities in enzyme-linked immunosorbent assay and Western blotting analysis using various human tissues. The results showed that the antibody induced by recombinant hLCN6 protein had high sensitivity and specificity. Taken together, the recombinant hLCN6 protein and mAb against this protein obtained from ou...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 16, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody 1C11 Mouse Monoclonal Antibody Against Mortalin.
Authors: PMID: 28806154 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 16, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody 5F11G11.
Authors: PMID: 28806155 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - August 16, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

DaMab-2: Anti-Human DGK α Monoclonal Antibody for Immunocytochemistry.
In this study, we immunized mice with recombinant DGKα and developed several anti-DGKα monoclonal antibodies (mAbs). One of the established anti-DGKα mAbs is a clone DaMab-2 (mouse IgG1, kappa). In enzyme-linked immunosorbent assay, DaMab-2 recognized only DGKα, and did not react with the other isozymes, such as DGKγ, DGKζ, DGKη, and DGKδ. Importantly, DaMab-2 is very useful in immunocytochemical analysis of human cultured cells, indicating that DaMab-2 is advantageous to analyze the localization and function of DGKα. PMID: 28742439 [PubMed - as supplied by publisher] ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 27, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PMab-52: Specific and Sensitive Monoclonal Antibody Against Cat Podoplanin for Immunohistochemistry.
In this study, we immunized mice with Chinese hamster ovary (CHO)-K1 cell lines expressing cPDPN, and developed anti-cPDPN mAbs. One of the clones, PMab-52 (IgM, kappa), detected cPDPN specifically in flow cytometry and Western blot analysis. PMab-52 is also useful for detecting feline squamous cell carcinoma cells in immunohistochemical analysis. PMab-52 is expected to be useful for investigating the function of cPDPN in feline carcinomas. PMID: 28737447 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 26, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Production of a Mouse Monoclonal Antibody Against Mortalin by Whole Cell Immunization.
In conclusion, pancreatic cancer is a fatal malignancy with no reliable biomarker for early screening and diagnosis. In this study, by establishing a pancreatic cell line, a panel of monoclonal antibodies was generated aiming to explore specific or associated cancer targets. We then introduced 1C11 monoclonal antibody that can specifically recognize mortalin as a main tumor marker and may serve as a new tool for diagnostic kit and therapeutic strategies targeting this molecule. PMID: 28719245 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 20, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

H2Mab-77 is a Sensitive and Specific Anti-HER2 Monoclonal Antibody Against Breast Cancer.
In this study, we developed novel anti-HER2 monoclonal antibodies (mAbs) and characterized their efficacy in flow cytometry, Western blot, and immunohistochemical analyses. Initially, we expressed the full length or ectodomain of HER2 in LN229 glioblastoma cells and then immunized mice with ectodomain of HER2 or LN229/HER2, and performed the first screening by enzyme-linked immunosorbent assays using ectodomain of HER2. Subsequently, we selected mAbs according to their efficacy in flow cytometry (second screening), Western blot (third screening), and immunohistochemical analyses (fourth screening). Among 100 mAb clones, on...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 14, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Specific Monoclonal Antibody Against Bcr/Abl Out-of-Frame Alternative Proteins as Diagnostic Tool in Chronic Myelogenous Leukemia Patients.
Authors: Casnici C, Crotta K, Volpe G, Panuzzo C, Lattuada D, Mesiano G, Saglio G, Marelli O Abstract More recently, alternative splicing of specific genes are investigated for their therapeutic potential. In particular, we reported the existence of BCR-ABL alternative splicing isoforms, in about 80% of Philadelphia-positive patients, which lead to the expression of aberrant proteins. These fusion proteins are characterized by an orphan initial and correct Bcr portion attached to a 112 amino acid sequence, arising from the impairment in the reading frame (reading of ABL exon 4 and 5). We demonstrated that these Abl...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 14, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Antipodocalyxin Antibody chPcMab-47 Exerts Antitumor Activity in Mouse Xenograft Models of Colorectal Adenocarcinomas.
Authors: Kaneko MK, Kunita A, Yamada S, Nakamura T, Yanaka M, Saidoh N, Chang YW, Handa S, Ogasawara S, Ohishi T, Abe S, Itai S, Harada H, Kawada M, Nishioka Y, Fukayama M, Kato Y Abstract Podocalyxin (PODXL) is expressed in several cancers, including brain tumors and colorectal cancers. PODXL overexpression is an independent predictor of progression, metastasis, and poor outcome. We recently immunized mice with recombinant human PODXL, which was produced using LN229 glioblastoma cells, and produced a clone PcMab-47 that could be used for investigating PODXL expression by flow cytometry and immunohistochemical anal...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - July 1, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Against Toxocara cati Second-Stage Larvae.
Authors: PMID: 28636499 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 23, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Association of rs1047763 and rs1008898 of C1GALT1 with IgA Nephropathy Risk: A Global Meta-Analysis.
Authors: Nie R, Cheng G, Zhang J, Dong Y, Wang C, Liu J, Qin X Abstract IgA nephropathy (IgAN) is a globally common primary glomerulonephritis characterized by an elevated level of serum IgA and immune complex deposition in the mesangial area. In the serum of patients with IgAN, the hinge region of IgA1 immunoglobulin contains aberrantly glycosylated O-glycans deficient in galactose, which is normally added to the core 1 O-glycan structure by core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1), the key enzyme in the process of glycosylation. It is unknown if single-nucleotid...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 23, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibodies Against Canine Distemper Virus.
Authors: PMID: 28636501 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 23, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody TX94, Human DNAX Accessory Molecule-1 (CD226).
Authors: PMID: 28636502 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 23, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Fine Epitope Mapping of Monoclonal Antibodies to the DNA Repair Protein, RadA.
Authors: Stuart MK, Hudman DA, Nachtrab SN, Hiatt JL, Seo J, Pullen SJ, Sargentini NJ Abstract Repair of DNA damage is vital to the health and survival of all organisms. In Escherichia coli, a protein known as RadA (or Sms) participates in recombinational repair, a process that uses an undamaged DNA strand in one DNA duplex to fill a gap in a homologous DNA strand in a sister DNA duplex. In a prior report, we described the production of monoclonal antibodies (MAbs) specific for RadA. Here, we investigated the epitopes recognized by two of the antibodies, MAbs 6F5 and 2A2. Premature stop codons (ochre mutations) wer...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 7, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Specific Monoclonal Antibodies Recognizing the Endogenous Chicken High Mobility Group Box 1 Protein.
Authors: Qu Y, Zhan Y, Yang S, Qiu X, Tan L, Sun Y, Meng C, Song C, Yu S, Ding C Abstract High mobility group box 1 (HMGB1) is a key member of the "danger associated molecular patterns" (DAMPs), which can localize in various compartments of the cell, and plays important roles in systemic inflammation. In the present study, monoclonal antibodies (MAbs) specifically against chicken HMGB1 were generated. The open reading frame of chicken HMGB1 was amplified by RT-PCR and cloned into the prokaryotic expression vector pET-28a to construct a recombinant plasmid pET-chHMGB1. The recombinant chicken HMGB1 protein...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 3, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Myeloma-Derived Light Chain Paired with a Diagnostic Monoclonal Antibody Hinders Immunoassay Performance.
We describe one such scenario where the P3U1 (P3X63Ag8U.1) myeloma fusion partner was used in the generation of a hybridoma producing protein induced vitamin K absence/antagonist-II (PIVKA II) antibody. The hybridoma produces three subpopulations of immunoglobulin as determined by ion exchange (IEx) chromatography that exhibit varying degrees of immunoreactivity (0%, 50%, or 100%) to the target antigen as determined by Surface Plasmon Resonance. To produce an antibody with the highest possible sensitivity and specificity, the antigen-specific heavy and light chain variable domains (VH and VL) were cloned from the hybridoma...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - June 1, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Investigation of Methylenetetrahydrofolate Reductase C677T Polymorphism and Human Papilloma Virus Genotypes in Iranian Breast Cancer.
In this study, we investigated HPV genotypes associated with BCs and its relation with MTHFR C677T polymorphism for early detection of familial BCs. A total of 84 archival BC samples from Iran were collected. Verification of each cancer reported in a relative was sought through the pathology reports of the hospital records. Then, DNA was extracted from all samples by standard methods and HPV genotypes and MTHFR C677T polymorphism genotypes were analyzed using multiplex polymerase chain reaction (PCR) and amplification-refractory mutation system (ARMS)-PCR. Finally, data analysis was performed using version 7 of the Epi Inf...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 27, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

ChLpMab-23: Cancer-Specific Human-Mouse Chimeric Anti-Podoplanin Antibody Exhibits Antitumor Activity via Antibody-Dependent Cellular Cytotoxicity.
In this study, we first determined the minimum epitope of LpMab-23 and revealed that Gly54-Leu64 peptide, especially Gly54, Thr55, Ser56, Glu57, Asp58, Arg59, Tyr60, and Leu64 of podoplanin, is a critical epitope of LpMab-23. We further produced human-mouse chimeric LpMab-23 (chLpMab-23) and investigated whether chLpMab-23 exerts antibody-dependent cellular cytotoxicity (ADCC) and antitumor activity. In flow cytometry, chLpMab-23 showed high sensitivity against a podoplanin-expressing glioblastoma cell line, LN319, and an oral cancer cell line, HSC-2. chLpMab-23 also showed ADCC activity against podoplanin-expressing CHO c...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 16, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of Novel Monoclonal Antibodies Against Human DNAM-1.
In this study, we generated and characterized five novel clones of anti-human DNAM-1 mAbs, named TX94, TX95, TX96, TX107, and TX108. Among these mAbs, TX94 is a unique neutralizing mAb that most efficiently blocked the interaction between DNAM-1 and CD155. Furthermore, TX94 inhibited NK cell-mediated cytotoxicity against a tumor cell line and suppressed CD8(+) T cell proliferation mediated by allogeneic mixed lymphocyte reaction. Thus, TX94 may be useful for molecular therapy targeting DNAM-1. PMID: 28498033 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 14, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Characterization of the Anti-Bovine Podoplanin Monoclonal Antibody PMab-44.
In this study, we determined the critical epitope of PMab-44 for the recognition of bovPDPN using many deletion mutants and point mutants of bovPDPN. Flow cytometric analyses revealed that the epitope of PMab-44 was Glu46-Thr50, which corresponds to platelet aggregation-stimulating (PLAG) domain-3. The important amino acids in the PMab-44 epitope were determined to be Glu46, Tyr48, and Thr50. Western blot analysis also confirmed these results, indicating that the PLAG domain of bovPDPN is also important in immunogenicity for producing useful anti-PDPN mAbs. PMID: 28498096 [PubMed - as supplied by publisher] (Source: Mo...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 14, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of Canine Distemper Virus Monoclonal Antibodies.
Authors: Liu Y, Hao L, Li X, Wang L, Zhang J, Deng J, Tian K Abstract Five canine distemper virus monoclonal antibodies were developed by immunizing BALB/c mice with a traditional vaccine strain Snyder Hill. Among these monoclonal antibodies, four antibodies recognized both field and vaccine strains of canine distemper virus without neutralizing ability. One monoclonal antibody, 1A4, against hemagglutinin protein of canine distemper virus was found to react only with vaccine strain virus but not field isolates, and showed neutralizing activity to vaccine strain virus. These monoclonal antibodies could be very usefu...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - May 5, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody: 5C9H2 Against Human Ghrelin Receptor.
Authors: PMID: 28430078 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody AbCR1 Against Guinea Pig CXCR1 and Monoclonal Antibody AbCR2 Against Guinea Pig CXCR2.
Authors: PMID: 28430079 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Characterization of Inhibitory Antibodies Specific to Guinea Pig CXCR1 and CXCR2.
In conclusion, we successfully obtained inhibitory antibodies specific to gpCXCR1 and gpCXCR2. These inhibitory antibodies will be useful to clarify the physiological roles of CXCR1 and CXCR2 in guinea pigs. PMID: 28430080 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 22, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Application of Monoclonal Antibody Against Lycopene.
Authors: Tsibezov VV, Bashmakov YK, Pristenskiy DV, Zigangirova NA, Kostina LV, Chalyk NE, Kozlov AY, Morgunova EY, Chernyshova MP, Lozbiakova MV, Kyle NH, Petyaev IM Abstract A monoclonal antibody (Mab) against lycopene was developed from hybridoma clones obtained from BALB/c mice immunized with trans-isomer of lycopene (t-lycopene, t-LC) conjugated with colloidal gold particles. An alternating immunization schedule which included injection of both formulations of immunogen (without and with Freund's adjuvant) was most effective in the elucidation of a measurable immune response to the t-Lycopene conjugate. Select...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 15, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Against Premembrane Viral Protein of Avian Tembusu Virus.
Authors: Huang X, Shi T, Mo K, Wang D, Peng X, Liao M, Zhou J Abstract Premembrane (prM) is a viral protein of flavivirus, which is important for the generation of infectious virion and for virus infection to the host. However, the biological properties and function of the prM of Avian Tembusu virus (ATMUV) have scarcely been studied to date. Monoclonal antibodies (mAbs) are a powerful tool for functional analysis of viral protein. To produce a mAb against prM protein of ATMUV, the prM gene sequence was amplified by reverse transcription polymerase chain reaction (RT-PCR) and cloned into the prokaryotic expression ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 15, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Characterization of a Monoclonal Antibody Specific for the Growth Hormone Secretagogue Receptor.
In this study, we characterized a new GHSR specific monoclonal antibody recognizing specifically the ghrelin receptor. This could be a useful tool for immunoassays aimed at obtaining insights into the physiological and pathological significance of the GHSR/ghrelin system. PMID: 28409695 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 15, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

LpMab-23: A Cancer-Specific Monoclonal Antibody Against Human Podoplanin.
Authors: Yamada S, Ogasawara S, Kaneko MK, Kato Y Abstract Human podoplanin (hPDPN), the ligand of C-type lectin-like receptor-2, is involved in cancer metastasis. Until now, many monoclonal antibodies (mAbs) have been established against hPDPN. However, it is still difficult to develop a cancer-specific mAb (CasMab) against hPDPN because the protein sequence of hPDPN expressed in cancer cells is the same as that in normal cells. Herein, we report LpMab-23 of the mouse IgG1 subclass, a novel CasMab against hPDPN. In an immunohistochemical analysis, LpMab-23 reacted with tumor cells of human oral cancer, but did not...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 9, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin.
In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb. Immunohistochemical analysis shows that mPMab-1 detects podocytes of the kidney, lymphatic endothelial cells of the colon, and type I alveolar cells of the lung. Importantly, mPMab-1 is more sensitive than PMab-1. This conversion strategy from rat mAb to mouse mAb could be applicable to other mAbs. PMID: 28387612 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 9, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry.
Authors: Ogasawara S, Kaneko MK, Yamada S, Honma R, Nakamura T, Saidoh N, Yanaka M, Yoshida K, Fujii Y, Kato Y Abstract Podocalyxin (PODXL) is a CD34-related sialomucin and a well-known marker of embryonic stem cells. PODXL is expressed in many types of tumors including colorectal cancers, breast cancers, and brain tumors. Overexpression of PODXL is an independent predictor of progression, metastasis, and poor outcome. PODXL is also expressed in many normal cells such as renal podocytes and endothelial cells (ECs). However, high-sensitive and high-specific anti-PODXL monoclonal antibodies (mAbs) have not been estab...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - April 8, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.
In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification. PMID: 28339303 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 26, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody TfR Monoclonal Antibody.
Authors: PMID: 28234554 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 27, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody 4E3-2F7 Antimouse Dermokine- β/α.
Monoclonal Antibody 4E3-2F7 Antimouse Dermokine-β/α. Monoclon Antib Immunodiagn Immunother. 2017 Feb;36(1):36 Authors: PMID: 28234555 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 27, 2017 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research