Expression Depression of CD300LG-γ in Human Pulmonary Carcinoma.
Expression Depression of CD300LG-γ in Human Pulmonary Carcinoma. Monoclon Antib Immunodiagn Immunother. 2016 Mar 15; Authors: Zhai S, Xu H, Jiang X, Sun S, Qin L, Wei D, Hu L Abstract The expression of CD300LG-γ at mRNA and protein level was detected by semiquantitative reverse transcription-polymerase chain reaction (PCR), quantitative real-time PCR, and western blot, and compared by t-test analysis. The results of semiquantitative reverse transcription-PCR showed that the mRNA expression of CD300LG-γ was significantly lower in pulmonary carcinoma tissues, compared to tumor-adjacent ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 17, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Fluorescence Immunoassay for Cocaine Detection.
Authors: Nakayama H, Kenjjou N, Shigetoh N, Ito Y Abstract A fluorescence immunoassay (FIA) has been developed for the detection of cocaine using norcocaine labeled with merocyanine dye and a monoclonal antibody specific to cocaine. Using this FIA, the detection range for cocaine was between 20.0 and 1700 μg/L with a limit of detection of 20.0 μg/L. Other cocaine derivatives did not interfere significantly with the detection when using this immunoassay technique with cross-reactivity values of less than 20%. Thus this FIA could be considered a useful tool for the detection of cocaine. PMID: ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 17, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Preparation of Monoclonal Antibodies and a Simple Myeloperoxidase-Immunosorbent Assay for Detecting Human Myeloperoxidase.
Authors: Bian ZP, Li XZ, Wu HF, Xu JD, Gu CR, Chen XJ, Yang D Abstract Myeloperoxidase (MPO), a leukocyte hemoprotein released from neutrophils, is thought to be a potential participant in plaque formation and plaque rupture. Therefore, MPO is regarded as an early marker predicting the risk for atherosclerosis, especially for coronary artery disease and acute coronary syndrome. We generated hybridoma clones 1E3 and 3E8 secreting monoclonal antibodies (mAbs) specific to human MPO. BALB/c mice were immunized with MPO protein purified from human neutrophils. Splenocytes from these mice were fused with the mouse myelom...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 17, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Characterization of a Novel Anti-Human HB-EGF Monoclonal Antibody Applicable for Paraffin-Embedded Tissues and Diagnosis of HB-EGF-Related Cancers.
In this study, we generated a clone of hybridoma-derived mAb 2-108 by immunizing mice with recombinant human HB-EGF protein expressed by human cells. The mAb 2-108 specifically bound to human HB-EGF but not to mouse HB-EGF and was successful in immunoblotting, even under reducing conditions, immunoprecipitation, and immunofluorescence for unfixed as well as paraformaldehyde-fixed cells. Notably, this mAb was effective in IHC of paraffin-embedded tumor specimens. Epitope mapping analysis showed that mAb 2-108 recognized the N-terminal prodomain in HB-EGF. These results indicate that this new anti-HB-EGF mAb 2-108 would be u...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 15, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Novel Monoclonal Antibody LpMab-17 Developed by CasMab Technology Distinguishes Human Podoplanin from Monkey Podoplanin.
Authors: Kato Y, Ogasawara S, Oki H, Honma R, Takagi M, Fujii Y, Nakamura T, Saidoh N, Kanno H, Umetsu M, Kamata S, Kubo H, Yamada M, Sawa Y, Morita KI, Harada H, Suzuki H, Kaneko MK Abstract Podoplanin (PDPN) is a type-I transmembrane sialoglycoprotein, which possesses a platelet aggregation-stimulating (PLAG) domain in its N-terminus. Among the three PLAG domains, O-glycan on Thr52 of PLAG3 is critical for the binding with C-type lectin-like receptor-2 (CLEC-2) and is essential for platelet-aggregating activity of PDPN. Although many anti-PDPN monoclonal antibodies (mAbs) have been established, almost all mAbs bi...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - March 6, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

OX130 Monoclonal Antibody Recognizes Human SIRPβ1 but Cross-Reacts on SIRPα from One Allele.
We describe the production of a monoclonal antibody (MAb) called OX130 that recognizes both common alleles of the human activating SIRPβ1 receptor but also cross-reacts with one of the common alleles of the inhibitory human SIRPα receptor. Thus one might get different outcomes when this MAb is used in assays from different individuals and shows the importance of characterizing SIRP MAb in this way. PMID: 26871367 [PubMed - as supplied by publisher] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Trastuzumab-Resistant Luminal B Breast Cancer Cells Show Basal-Like Cell Growth Features Through NF-κB-Activation.
Trastuzumab-Resistant Luminal B Breast Cancer Cells Show Basal-Like Cell Growth Features Through NF-κB-Activation. Monoclon Antib Immunodiagn Immunother. 2016 Feb;35(1):1-11 Authors: Kanzaki H, Mukhopadhya NK, Cui X, Ramanujan VK, Murali R Abstract A major clinical problem in the treatment of breast cancer is mortality due to metastasis. Understanding the molecular mechanisms associated with metastasis should aid in designing new therapeutic approaches for breast cancer. Trastuzumab is the main therapeutic option for HER2+ breast cancer patients; however, the molecular basis for trastuzumab resis...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Analysis of aDR5scFv with Specific Identification and Function.
In this study, an aDR5scFv was constructed and expressed, and it was conformed so that it could recognize and bind eDR5 specifically. The therapeutic effects on human lung adenocarcinoma cells lines 973 in vitro and in vivo were detected by MTT assay, flow cytometry, hematoxylin and eosin staining, and TUNEL assay. aDR5scFv was able to induce 973 cell apoptosis in an in vitro system. The protein expressions of caspase-3, Bax, and cytochrome c were raised, and aDR5scFv also inhibited tumor growth in mice with its effect as well as with radiotherapy. It is concluded that aDR5scFv could possibly be considered as a novel thera...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of Monoclonal Antibodies Against NS1 Protein of Duck Tembusu Virus.
Authors: Ti J, Li Z, Zhao D, Chen H, Li F, Diao Y Abstract To produce monoclonal antibodies (MAbs) against NS1 protein of duck Tembusu virus (TMUV), the NS1 gene sequence was cloned into the prokaryotic expression vector pET-28a(+). The prokaryotic protein NS1 was successfully expressed in BL21 (DE3) and used as the immunogen in mice. Six MAbs against NS1 protein were obtained by using the standard hybridoma technique. All MAbs can react with the denatured NS1 protein in the Western blot assay and the native NS1 protein from the TMUV-infected BHK-21 cells in the immunofluorescence assay. The ELISA titers of the cel...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and Characterization of Monoclonal Antibodies Specific for 3-(1-naphthoyl) Indole Derivatives.
Authors: Nakayama H, Kenjyou N, Ito Y Abstract 3-(1-naphthoyl) indole is one of the raw materials that synthesizes a synthetic cannabinoid such as 1-pentyl-3-(1-naphthoyl) indole (JWH-018) and 1-butyl-3-(1-naphthoyl) indole (JWH-073). It is important to detect the 3-(1-naphthoyl) indole derivatives rapidly, sensitively, and comprehensively. We developed two monoclonal antibodies (MAb) against 3-(1-naphthoyl) indole derivatives, named NT1 (IgG1) and NT2 (IgG1), which were possibly effective for detecting 3-(1-naphthoyl) indole derivatives. The cross-reactive ability of these MAbs was evaluated using a competitive en...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus S1 Protein by Recombinant Protein Antigen.
Authors: PMID: 26871515 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody NT1 Against 3-(1-naphthoyl) Indole Derivatives.
Authors: PMID: 26871516 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody NT2 Against 3-(1-naphthoyl) Indole Derivatives.
Authors: PMID: 26871517 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody 1.82 Against Ducreyi Serum Resistance A.
Authors: PMID: 26871518 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody 6.95 Against Ducreyi Serum Resistance A.
Authors: PMID: 26871519 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 14, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Isolation of 1E4 IgM Anti-Fasciola hepatica Rediae Monoclonal Antibody from Ascites: Comparison of Two Purification Protocols.
Authors: Alba A, Marcet R, Otero O, Hernández HM, Figueredo M, Sarracent J Abstract Purification of immunoglobulin M (IgM) antibodies could be challenging, and is often characterized by the optimization of the purification protocol to best suit the particular features of the molecule. Here, two different schemes were compared to purify, from ascites, the 1E4 IgM monoclonal antibody (mAb) previously raised against the stage of redia of the trematode Fasciola hepatica. This immunoglobulin is used as capture antibody in an immunoenzymatic assay to detect parasite ongoing infection in its intermediate hos...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - February 2, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of Splice Variant-Specific Monoclonal Antibodies Against Human α3 Integrin.
Development of Splice Variant-Specific Monoclonal Antibodies Against Human α3 Integrin. Monoclon Antib Immunodiagn Immunother. 2016 Jan 20; Authors: Oku T, Ando Y, Ogura M, Tsuji T Abstract Integrins are a major family of adhesion molecules, consisting of heterodimers (α and β subunits). Several reports have suggested the presence of splice variants in the cytoplasmic domain of certain integrin subunits. In the present study, we detected mRNA of integrin α3 splice variants (α3A and α3B) by RT-PCR using total RNA from the human brain as a template. The α3B varian...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 22, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of a Novel Functional Monoclonal Antibody to Human CD275: Characterization and Biological Activity.
In this report, a novel mouse anti-human CD275 monoclonal antibody (MAb) was prepared using hybridoma technology, and immunological characteristics of the MAb were determined. The results showed that the MAb (clone 13D11) was IgG2(κ) and bound specifically to human CD275. By mutual competition, we found that the antibody recognized different epitopes of CD275 antigen compared with commercial antibodies and could block ICOS-CD275 interaction. Crosslinking of CD275 with MAb 13D11 markedly blocked ICOS positive signal and inhibited T cell proliferation and cytokine production. In addition, the 13D11 MAb was suitable for...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 22, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of Novel Monoclonal Antibody PMab-32 Against Rabbit Podoplanin.
In this study, we immunized mice with the recombinant proteins of rabPDPN, and developed a novel anti-rabPDPN MAb, named PMab-32. PMab-32 could detect endogenous and exogenous rabPDPN in flow cytometry and Western blot analysis. The KD of PMab-32 was determined to be 6.2 × 10(-8) M by flow cytometry. Immunohistochemical analysis showed that PMab-32 is useful for detecting podocytes, type I alveolar cells, and lymphatic endothelial cells in normal rabbit tissues. PMab-32 is expected to be useful for various rabbit experiments. PMID: 26788987 [PubMed - as supplied by publisher] (Source: Monoclonal Ant...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 22, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of a Neutralizing Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus S1 Protein.
Authors: Zhang Y, Yao Y, Gao X, Wang Y, Jia X, Xiao Y, Wang T, Li X, Tian K Abstract The truncated spike protein (S1, 390-789aa) of porcine epidemic diarrhea virus was expressed as a recombinant protein in Baculorvirus expression system. Female BALB/c mice were immunized with the purified recombinant S1 (rS1) protein and two monoclonal antibodies, designated 8A3A10 and 12C4G12, were generated by hybridoma technique. The two monoclonal antibodies specifically reacted with PEDV rS1 protein, as proven by Western blot and indirect immunofluorescence assay. 8A3A10 monoclonal antibody showed a neutralizing activity to PE...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - January 17, 2016 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

CCL22-specific Antibodies Reveal That Engagement of Two Distinct Binding Domains on CCL22 Is Required for CCR4-mediated Function.
Authors: Santulli-Marotto S, Wheeler J, Lacy ER, Boakye K, Luongo J, Wu SJ, Ryan M Abstract CCL22 inactivation in vivo occurs by cleavage at the N-terminus; however, it is unclear whether this encompasses the entire site of CCR4 interaction. CCL17 also binds CCR4 and its function requires binding via two discrete binding sites. Using monoclonal antibodies (MAbs), we report that there are two separate sites on CCL22 that are required for CCR4-mediated function. The CCL22-specific antibodies bind with affinities of 632 ± 297 pM (MC2B7) and 308 ± 43 pM (MAB4391) and neither ex...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Inducing Humoral Immune Responses Against Regulatory T Cells by Foxp3-Fc(IgG) Fusion Protein.
Authors: Niri NM, Hadjati J, Sadat M, Memarnejadian A, Aghasadeghi M, Akbarzadeh A, Zarghami N Abstract The existence of a developed network of suppressory factors and cells against an immune response in different cancers has been proven; regulatory T cells are a typical issue. Therefore their depletion, elimination, or suppression has been assessed in different research studies that were not entirely successful. By applying an improved vaccine against regulatory T cells, we have evaluated the B cell response elicited by the vaccine in an experimental design. A previously described DNA vaccine and recombinant prote...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibodies Against Porcine sIgA and Their Use in Immunohistochemistry.
In this study, BALB/c mice were immunized with porcine sIgA and the splenocytes were then fused with myeloma cells. Finally, three hybridoma cell lines secreting monoclonal antibody (MAb) against porcine sIgA were obtained. All three MAbs had no cross-reaction with porcine IgG confirmed by Western blot analysis. Furthermore, lungs, tracheas, and intestines were collected from healthy porcines to prepare tissue slices, followed by incubation with the MAb produced in this study. The results showed that sIgA existing in respiratory and digestive systems could be detected by this newly produced MAb. These generated MAbs agains...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Production and Characterization of a Novel Monoclonal Antibody Against Human Sortilin.
Authors: Ghaemimanesh F, Bayat AA, Babaei S, Ahmadian G, Zarnani AH, Behmanesh M, Jeddi-Tehrani M, Rabbani H Abstract Sortilin, as a member of Vps10p-domain sorting receptor family, is overexpressed in a number of malignancies, including ovarian carcinoma. Antibodies against sortilin may contribute to further clarification of sortilin functional activities in signal transduction, intracellular sorting of proteins, and endocytosis. The aim of this study was to produce a monoclonal antibody against a synthetic peptide derived from extracellular N-terminal region of sortilin to be used as a tool for investigating sort...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development of Sensitive Monoclonal Antibody PMab-2 Against Rat Podoplanin.
Authors: Oki H, Honma R, Ogasawara S, Fujii Y, Liu X, Takagi M, Kaneko MK, Kato Y Abstract Podoplanin (PDPN) is a platelet aggregation-inducing factor, which is known as an endogenous ligand of C-type lectin-like receptor-2 (CLEC-2). PDPN is also expressed in several normal tissues, such as lung type I alveolar cells and kidney podocytes. Although many monoclonal antibodies (MAbs) against human PDPN (hPDPN) or mouse PDPN (mPDPN) have been established, anti-rat PDPN (rPDPN) MAbs, especially against platelet aggregation-stimulating (PLAG) domain (29-54 amino acids) of rPDPN, have not been developed. Therefore, functi...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Design and Generation of Humanized Single-chain Fv Derived from Mouse Hybridoma for Potential Targeting Application.
In this study, a concise humanization strategy combined with an optimized production method for humanizing scFvs was successfully employed. Two antibody clones, one directed against the hemagglutinin of H5N1 influenza virus, the other against EpCAM, a cancer biomarker, were used to demonstrate the validity of the method. Heavy chain (VH) and light chain (VL) variable regions of immunoglobulin genes from mouse hybridoma cells were sequenced and subjected to the construction of mouse scFv 3-D structure. Based on in silico modeling, the humanized version of the scFv was designed via complementarity-determining region (CDR) gr...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor.
Authors: Osaki T, Wang CX, Tachibana T, Azuma M, Kitamura M, Nakanishi T Abstract Overexpression of the epidermal growth factor receptor (EGFR) gene and dysregulation of EGFR signaling are observed in various cancer cells, and EGFR is a validated target for cancer therapy. In the present study, we report on the generation of two rat anti-EGFR antibodies (clones 2C2D3 and 4H7F4) by using the rat lymph node method. Flow cytometric analysis and immunofluorescence showed that both antibodies specifically bound to EGFR on the surface of cancer cells. Competitive analysis demonstrated that the epitope of each antibody ha...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Establishment of a Mouse Anti-ovalbumin IgE Monoclonal Antibody That Induces FcɛRII (CD23)-dependent Activation Without FcɛRI-Dependent Activation.
Authors: Yamaki K, Yoshino S Abstract IgE mainly activates cells via two receptors, FcɛRI and FcɛRII. Blocking antibodies against and animals genetically targeted for these receptors have been successfully used to distinguish between these two activating pathways. In the present study, we investigated whether our newly established anti-ovalbumin (OVA) monoclonal IgE OE-2 induced FcɛRII-dependent activation, but not FcɛRI-dependent activation in vivo and in vitro, in contrast to the previously established anti-OVA IgE OE-1, which stimulated FcɛRI and FcɛRII. The FcɛRI-mediated degranulation of RBL2H3 cells an...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Preparation of Monoclonal Antibodies Against SpiC Protein Secreted by T3SS-2 of Salmonella spp.
Authors: Geng S, Qian S, Pan Z, Sun L, Chen X, Jiao X Abstract SpiC protein, a member of Salmonella spp. type III secretion system (T3SS)-2, is necessary for the survival of Salmonella within macrophages, and it plays a vital role in Salmonella pathogenesis. To develop and test monoclonal antibodies (MAbs) against SpiC protein, two recombinant proteins, rHis-SpiC and rGST-SpiC, were expressed in vitro in the prokaryotic expression vectors pET-30(a) and pGEX-6p-1, respectively, and rHis-SpiC protein used to immunize mice. Hybridomas were generated from the splenocytes of these mice and the monoclonal antibodies prod...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Generation and Characterization of Monoclonal Antibodies Specific to Avian Influenza H5N1 Hemagglutinin Protein.
In this study, we describe the preliminary characterization of four murine MAbs developed against recombinant hemagglutinin (rHA) protein of avian H5N1 A/turkey/Turkey/1/2005 virus that are either highly specific or broadly reactive against HA from other H5N1 subtype viruses, such as A/Hong Kong/213/03, A/Common magpie/Hong Kong/2256/2006, and A/Barheaded goose/Quinghai/14/2008. The antibody binding is specific to H5N1 HAs, as none of the antibodies bound H1N1, H2N2, H3N2, or B/Brisbane/60/2008 HAs. Out of the four MAbs, one of them (MA-7) also reacted weakly with the rHA protein of H7N9 A/Anhui/1/2013. All four MAbs bound...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

OX123 Recognizes Mouse SIRPβ1.
OX123 Recognizes Mouse SIRPβ1. Monoclon Antib Immunodiagn Immunother. 2015 Dec;34(6):442 Authors: PMID: 26683185 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Peptide-based Monoclonal Antibody 4E5-A10 Against β-Actin.
Peptide-based Monoclonal Antibody 4E5-A10 Against β-Actin. Monoclon Antib Immunodiagn Immunother. 2015 Dec;34(6):443 Authors: PMID: 26683186 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Peptide-based Monoclonal Antibody 2D8 Against Human Sortilin.
Authors: PMID: 26683187 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Monoclonal Antibody Against SpiC Protein from Salmonella.
Authors: PMID: 26683188 [PubMed - in process] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 25, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The Wistar legacy to embryonic stem cell research.
Authors: Knowles BB PMID: 24892230 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Hilary Koprowski and the viral immunity hub at the 1980s Wistar Institute: a T cell epitope perspective.
Authors: Hackett CJ PMID: 24892373 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Genetic resistance to flaviviruses.
Authors: Brinton MA PMID: 24892926 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Cancer immunotherapeutics: evolution of monoclonal antibodies to peptide immunogens.
Authors: Murali R, Kieber-Emmons T Abstract The Wistar Institute under Hilary Koprowski's direction played a preeminent role in bringing the fruits of basic science to clinic through biotechnology and life science enterprises. Koprowski's early view on the utility of monoclonal antibodies has been validated to some extent, because monoclonal therapeutics form one of the fastest growing and most successful and lucrative segments within the biopharmaceutical sector. Over 30 monoclonal antibody drugs have now been approved in the United States and Europe. However, monoclonal antibodies might be viewed in the context o...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Association of varicella zoster virus with giant cell arteritis.
Authors: Gilden D PMID: 24893151 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Career lasting influences.
Authors: Bennink JR PMID: 24893278 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Hilary Koprowski, MD: A lifetime of work.
Authors: Steplewski Z, Kieber-Emmons T PMID: 24937247 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Hilary Koprowski, MD December 5, 1916-April 11, 2013.
Authors: Steplewski Z, Kieber-Emmons T PMID: 24937248 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Remembrances of Hilary Koprowski from a Surgeon's Perspective.
Authors: Sears HF PMID: 24937249 [PubMed - indexed for MEDLINE] (Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy)
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Nanotechnology-based approaches for the development of diagnostics, therapeutics, and vaccines.
Authors: Srinivasan A, Rastogi A, Ayyavoo V, Srivastava S Abstract The architecture of nanoparticles of biological origin, generally also known as bionanoparticles, presents several features that are ideal for their use in developing diagnostics, therapeutics, and vaccines. In this regard, particles formed by viral proteins using recombinant DNA technology resemble authentic virus particles. However, they lack infectivity due to the absence of genetic components such as DNA or RNA. Hence, they are designated as virus-like particles (VLP). VLPs possess the following characteristics: (1) they can be generated by eith...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Expression of recombinant vaccines and antibodies in plants.
Authors: Ko K Abstract Plants are able to perform post-translational maturations of therapeutic proteins required for their functional biological activity and suitable in vivo pharmacokinetics. Plants can be a low-cost, large-scale production platform of recombinant biopharmaceutical proteins such as vaccines and antibodies. Plants, however, lack mechanisms of processing authentic human N-glycosylation, which imposes a major limitation in their use as an expression system for therapeutic glycoproducts. Efforts have been made to circumvent plant-specific N-glycosylation, as well as to supplement the plant's endogeno...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

A monoclonal antibody against PMEL.
Authors: Shi F, Xu Z, Chen H, Wang X, Cui J, Zhang P, Zhang P, Xie X Abstract PMEL, also known as Pmel17 or gp100, is a melanocyte-specific glycoprotein that is essential for the formation of stage II melanosomes. As it has a highly restricted expression pattern in normal tissues and a transient presence on the cell surface, PMEL is believed to be a potential target for antibody drug conjugate therapy in some pigmentary diseases. The production of a high specificity and high affinity monoclonal antibody against human PMEL was helpful for the antibody drug conjugate therapy study. In the present study, monoclonal an...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

ERG monoclonal antibody in the diagnosis and biological stratification of prostate cancer: delineation of minimal epitope, critical residues for binding, and molecular basis of specificity.
Authors: Rastogi A, Tan SH, Banerjee S, Sharad S, Kagan J, Srivastava S, McLeod DG, Srivastava S, Srinivasan A Abstract Recently we reported the development of a highly specific murine monoclonal antibody (ERG MAb 9FY) against the ERG oncoprotein. ERG is expressed in over half of all prostate cancers (CaP) as a result of specific gene fusions involving ERG and the androgen regulated TMPRSS2 promoter. ERG MAb 9FY has been extensively used in the evaluations of CaP. Increasing use of ERG MAb in CaP has prompted us to characterize the precise ERG epitope it binds to and to define the molecular basis of its specificity...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Development and characterization of domain-specific monoclonal antibodies produced against human SLAMF9.
Authors: Volkova O, Guselnikov S, Mechetina L, Chikaev N, Baranov K, Kulemzin S, Reshetnikova E, Najakshin A, Taranin A Abstract SLAMF9 is a member of the signaling lymphocyte-activating molecule (SLAM) immunoreceptor family. The SLAM family receptors are expressed in a broad range of immune cells and play an important role in immunity. To date, SLAMF9 is the least studied member of this family. Its ligand, signaling properties, and cells on whose surface it is expressed are unknown. We generated hybridoma clones 6E11 and 7G5 secreting monoclonal antibodies specific to human SLAMF9. BALB/c mice were immunized with ...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

Successful construction and massive expression of a novel Anti-CD19 human-mouse chimeric antibody Hm2E8b.
Authors: Shen D, Tang Y, Li S, Xu W, Zhang L Abstract CD19 antigen is a major target for human B cell malignancies. Many studies have shown that the antibodies recognizing this antigen hold clinical therapeutic potential, while CD19 antibody of mouse origin requires genetic engineering to reduce the potential side effects of the antibody for their clinical use. There are many clones of CD19 antibodies available with different subclasses of immunoglobulin. IgM type antibody holds a high affinity and high complement activating capacities facilitating the targeting efficacy when it is used in targeting therapy. Howeve...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

The D-6 mouse monoclonal antibody recognizes the CD74 cytoplasmic tail.
Authors: Genève L, Gauthier C, Thibodeau J Abstract The invariant chain (Ii; CD74) is a multifunctional protein of the immune system and a major player in the presentation of exogenous antigens to T cells. In the endoplasmic reticulum (ER), Ii assists the folding and trafficking of MHC class II molecules. In the present study, we characterized the recently commercialized D-6 monoclonal antibody (MAb) made against a polypeptide spanning the entire sequence of the p33 isoform of human Ii. Using transgenic mice expressing the human p35 isoform, we showed by flow cytometry that D-6 only slightly cross-reacts wit...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 2, 2015 Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research