Validation of three qPCR for the detection of Burkholderia mallei in equine tissue samples

Arch Microbiol. 2021 May 24. doi: 10.1007/s00203-021-02367-7. Online ahead of print.ABSTRACTBurkholderia mallei is the causative agent of glanders, a zoonosis listed by the World Organization for Animal Health as of mandatory notification. In this work, a comparison of three qPCR protocols was made, two of them based on articles by other authors and one standardized in house, this last one aiming at a genomic region that does not exist in other species of the Burkholderia genus. All qPCRs showed high efficiency and good repeatability. However, reactions with Cq between 36 and 40 were considered suspicious and unreliable, requiring greater clinical criteria to analyze the results.PMID:34028570 | DOI:10.1007/s00203-021-02367-7

Source: Archives of Microbiology - May 24, 2021 Category: Microbiology Authors: Ant ônio Augusto Fonseca Júnior Carla Amaral Pinto Cid Arist óteles de Siqueira Alencar Bruna Lopes Bueno Jenner K Pimenta Dos Reis Maur ício Baltazar de Carvalho Filho Source Type: research