Fatty acid modified-antimicrobial peptide analogues with potent antimicrobial activity and topical therapeutic efficacy against Staphylococcus hyicus
This study provides evidence that conjugation of a fatty acid to antimicrobial peptides can improve the activity and have potential for topical therapeutic of S. hyicus skin infections. KEY POINTS: • Lin-Lfcin4NH2/Lfcin5NH2 showed stronger antimicrobial activity than parent peptides. • Lin-Lfcin4NH2/Lfcin5NH2 had a more effective ability to destroy bacterial membranes. • Lin-Lfcin4NH2/Lfcin5NH2 showed a topically higher efficacy than parent peptides.PMID:34319418 | DOI:10.1007/s00253-021-11454-0 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 28, 2021 Category: Microbiology Authors: He Liu Na Yang Da Teng Ruoyu Mao Ya Hao Xuanxuan Ma Xiumin Wang Jianhua Wang Source Type: research

The potential of Akkermansia muciniphila in inflammatory bowel disease
This article therefore aims to review the relationship between A. muciniphila and IBD, the effect of intervention of A. muciniphila on IBD, and the possible reasons for the contradictory role of A. muciniphila in the treatment of IBD. KEY POINTS: The effect of A. muciniphila on inflammatory bowel disease is controversy. A. muciniphila shows anti-inflammatory potential in IBD. The colitogenicity of A. muciniphila is context dependent.PMID:34312713 | DOI:10.1007/s00253-021-11453-1 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 27, 2021 Category: Microbiology Authors: Ting Zhang Xinghui Ji Gaochen Lu Faming Zhang Source Type: research

The impact of bZIP Atf1ortholog global regulators in fungi
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11431-7. Online ahead of print.ABSTRACTRegulation of signal transduction pathways is crucial for the maintenance of cellular homeostasis and organismal development in fungi. Transcription factors are key elements of this regulatory network. The basic-region leucine zipper (bZIP) domain of the bZIP-type transcription factors is responsible for DNA binding while their leucine zipper structural motifs are suitable for dimerization with each other facilitiating the formation of homodimeric or heterodimeric bZIP proteins. This review highlights recent knowledge on ...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Éva Leiter Tam ás Emri Klaudia P ákozdi L ászló Hornok Istv án Pócsi Source Type: research

Anaerobic hydrolysis of complex substrates in full-scale aerobic granular sludge: enzymatic activity determined in different sludge fractions
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11443-3. Online ahead of print.ABSTRACTComplex substrates, like proteins, carbohydrates, and lipids, are major components of domestic wastewater, and yet their degradation in biofilm-based wastewater treatment technologies, such as aerobic granular sludge (AGS), is not well understood. Hydrolysis is considered the rate-limiting step in the bioconversion of complex substrates, and as such, it will impact the utilization of a large wastewater COD (chemical oxygen demand) fraction by the biofilms or granules. To study the hydrolysis of complex substrates within t...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Sara Toja Ortega Mario Pronk Merle K de Kreuk Source Type: research

The impact of bZIP Atf1ortholog global regulators in fungi
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11431-7. Online ahead of print.ABSTRACTRegulation of signal transduction pathways is crucial for the maintenance of cellular homeostasis and organismal development in fungi. Transcription factors are key elements of this regulatory network. The basic-region leucine zipper (bZIP) domain of the bZIP-type transcription factors is responsible for DNA binding while their leucine zipper structural motifs are suitable for dimerization with each other facilitiating the formation of homodimeric or heterodimeric bZIP proteins. This review highlights recent knowledge on ...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Éva Leiter Tam ás Emri Klaudia P ákozdi L ászló Hornok Istv án Pócsi Source Type: research

Anaerobic hydrolysis of complex substrates in full-scale aerobic granular sludge: enzymatic activity determined in different sludge fractions
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11443-3. Online ahead of print.ABSTRACTComplex substrates, like proteins, carbohydrates, and lipids, are major components of domestic wastewater, and yet their degradation in biofilm-based wastewater treatment technologies, such as aerobic granular sludge (AGS), is not well understood. Hydrolysis is considered the rate-limiting step in the bioconversion of complex substrates, and as such, it will impact the utilization of a large wastewater COD (chemical oxygen demand) fraction by the biofilms or granules. To study the hydrolysis of complex substrates within t...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Sara Toja Ortega Mario Pronk Merle K de Kreuk Source Type: research

The impact of bZIP Atf1ortholog global regulators in fungi
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11431-7. Online ahead of print.ABSTRACTRegulation of signal transduction pathways is crucial for the maintenance of cellular homeostasis and organismal development in fungi. Transcription factors are key elements of this regulatory network. The basic-region leucine zipper (bZIP) domain of the bZIP-type transcription factors is responsible for DNA binding while their leucine zipper structural motifs are suitable for dimerization with each other facilitiating the formation of homodimeric or heterodimeric bZIP proteins. This review highlights recent knowledge on ...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Éva Leiter Tam ás Emri Klaudia P ákozdi L ászló Hornok Istv án Pócsi Source Type: research

Anaerobic hydrolysis of complex substrates in full-scale aerobic granular sludge: enzymatic activity determined in different sludge fractions
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11443-3. Online ahead of print.ABSTRACTComplex substrates, like proteins, carbohydrates, and lipids, are major components of domestic wastewater, and yet their degradation in biofilm-based wastewater treatment technologies, such as aerobic granular sludge (AGS), is not well understood. Hydrolysis is considered the rate-limiting step in the bioconversion of complex substrates, and as such, it will impact the utilization of a large wastewater COD (chemical oxygen demand) fraction by the biofilms or granules. To study the hydrolysis of complex substrates within t...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Sara Toja Ortega Mario Pronk Merle K de Kreuk Source Type: research

The impact of bZIP Atf1ortholog global regulators in fungi
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11431-7. Online ahead of print.ABSTRACTRegulation of signal transduction pathways is crucial for the maintenance of cellular homeostasis and organismal development in fungi. Transcription factors are key elements of this regulatory network. The basic-region leucine zipper (bZIP) domain of the bZIP-type transcription factors is responsible for DNA binding while their leucine zipper structural motifs are suitable for dimerization with each other facilitiating the formation of homodimeric or heterodimeric bZIP proteins. This review highlights recent knowledge on ...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Éva Leiter Tam ás Emri Klaudia P ákozdi L ászló Hornok Istv án Pócsi Source Type: research

Anaerobic hydrolysis of complex substrates in full-scale aerobic granular sludge: enzymatic activity determined in different sludge fractions
Appl Microbiol Biotechnol. 2021 Jul 24. doi: 10.1007/s00253-021-11443-3. Online ahead of print.ABSTRACTComplex substrates, like proteins, carbohydrates, and lipids, are major components of domestic wastewater, and yet their degradation in biofilm-based wastewater treatment technologies, such as aerobic granular sludge (AGS), is not well understood. Hydrolysis is considered the rate-limiting step in the bioconversion of complex substrates, and as such, it will impact the utilization of a large wastewater COD (chemical oxygen demand) fraction by the biofilms or granules. To study the hydrolysis of complex substrates within t...
Source: Applied Microbiology and Biotechnology - July 24, 2021 Category: Microbiology Authors: Sara Toja Ortega Mario Pronk Merle K de Kreuk Source Type: research

Assessment of Biolog Ecoplate < sup > TM < /sup > method for functional metabolic diversity of aerotolerant pig fecal microbiota
In this study, the BiologTM Ecoplates technology was applied for studying the metabolic potential of the aerotolerant microbial community of pig fecal samples, evaluating the interference of different storage conditions and cell concentrations. The length of time for which a fecal sample maintained detectable and unchanged microbial metabolic activity was also investigated. Two assays aimed to evaluate differences in the metabolic activities between fresh and snap-frozen fecal samples at different dilutions and at different lengths of times of preservation at -80°C were carried out. The biodiversity and the predicted f...
Source: Applied Microbiology and Biotechnology - July 23, 2021 Category: Microbiology Authors: A Checcucci D Luise M Modesto F Correa P Bosi P Mattarelli Paolo Trevisi Source Type: research

Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor
Appl Microbiol Biotechnol. 2021 Jul 22. doi: 10.1007/s00253-021-11441-5. Online ahead of print.ABSTRACTDuring the last few decades, whole-cell biosensors have attracted increasing attention for their enormous potential in monitoring bioavailable heavy metal contaminations in the ecosystem. Visual and measurable output signals by employing natural pigments have been demonstrated to offer another potential choice to indicate the existence of bioavailable heavy metals in recent years. The biosynthesis of the blue pigment indigoidine has been achieved in E. coli following heterologous expression of both BpsA (a single-module n...
Source: Applied Microbiology and Biotechnology - July 22, 2021 Category: Microbiology Authors: Chang-Ye Hui Yan Guo Li-Mei Li Lisa Liu Yu-Ting Chen Juan Yi Nai-Xing Zhang Source Type: research

MoCpa1-mediated arginine biosynthesis is crucial for fungal growth, conidiation, and plant infection of Magnaporthe oryzae
Appl Microbiol Biotechnol. 2021 Jul 22. doi: 10.1007/s00253-021-11437-1. Online ahead of print.ABSTRACTArginine is an important amino acid involved in processes such as cell signal transduction, protein synthesis, and sexual reproduction. To understand the biological roles of arginine biosynthesis in pathogenic fungi, we used Cpa1, the carbamoyl phosphate synthase arginine-specific small chain subunit in Saccharomyces cerevisiae as a query to identify its ortholog in the Magnaporthe oryzae genome and named it MoCpa1. MoCpa1 is a 471-amino acid protein containing a CPSase_sm_chain domain and a GATase domain. MoCpa1 transcri...
Source: Applied Microbiology and Biotechnology - July 22, 2021 Category: Microbiology Authors: Osakina Aron Min Wang Anjago Wilfred Mabeche Batool Wajjiha Meiqin Li Shuai Yang Haixia You Yan Cai Tian Zhang Yunxi Li Baohua Wang Dongmei Zhang Zonghua Wang Wei Tang Source Type: research

A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66
In this study, the latter enzyme was expressed in A. limacinum SR21, and both C16:1 n-7 and C18:1 n-7 could be detected in the transgenic cells. Our results show that this desaturase, annotated T66Des9, is a Δ9-desaturase accepting C16:0 as a substrate. Phylogenetic studies indicate that the corresponding gene probably has evolved from a Δ12-desaturase-encoding gene. This possibility has not been reported earlier and is important to consider when one tries to deduce the potential a given organism has for producing unsaturated fatty acids based on its genome sequence alone. KEY POINTS: • In thraustochytrids...
Source: Applied Microbiology and Biotechnology - July 22, 2021 Category: Microbiology Authors: E-Ming Rau Inga Marie Aasen Helga Ertesv åg Source Type: research

Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor
Appl Microbiol Biotechnol. 2021 Jul 22. doi: 10.1007/s00253-021-11441-5. Online ahead of print.ABSTRACTDuring the last few decades, whole-cell biosensors have attracted increasing attention for their enormous potential in monitoring bioavailable heavy metal contaminations in the ecosystem. Visual and measurable output signals by employing natural pigments have been demonstrated to offer another potential choice to indicate the existence of bioavailable heavy metals in recent years. The biosynthesis of the blue pigment indigoidine has been achieved in E. coli following heterologous expression of both BpsA (a single-module n...
Source: Applied Microbiology and Biotechnology - July 22, 2021 Category: Microbiology Authors: Chang-Ye Hui Yan Guo Li-Mei Li Lisa Liu Yu-Ting Chen Juan Yi Nai-Xing Zhang Source Type: research

MoCpa1-mediated arginine biosynthesis is crucial for fungal growth, conidiation, and plant infection of Magnaporthe oryzae
Appl Microbiol Biotechnol. 2021 Jul 22. doi: 10.1007/s00253-021-11437-1. Online ahead of print.ABSTRACTArginine is an important amino acid involved in processes such as cell signal transduction, protein synthesis, and sexual reproduction. To understand the biological roles of arginine biosynthesis in pathogenic fungi, we used Cpa1, the carbamoyl phosphate synthase arginine-specific small chain subunit in Saccharomyces cerevisiae as a query to identify its ortholog in the Magnaporthe oryzae genome and named it MoCpa1. MoCpa1 is a 471-amino acid protein containing a CPSase_sm_chain domain and a GATase domain. MoCpa1 transcri...
Source: Applied Microbiology and Biotechnology - July 22, 2021 Category: Microbiology Authors: Osakina Aron Min Wang Anjago Wilfred Mabeche Batool Wajjiha Meiqin Li Shuai Yang Haixia You Yan Cai Tian Zhang Yunxi Li Baohua Wang Dongmei Zhang Zonghua Wang Wei Tang Source Type: research

A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66
In this study, the latter enzyme was expressed in A. limacinum SR21, and both C16:1 n-7 and C18:1 n-7 could be detected in the transgenic cells. Our results show that this desaturase, annotated T66Des9, is a Δ9-desaturase accepting C16:0 as a substrate. Phylogenetic studies indicate that the corresponding gene probably has evolved from a Δ12-desaturase-encoding gene. This possibility has not been reported earlier and is important to consider when one tries to deduce the potential a given organism has for producing unsaturated fatty acids based on its genome sequence alone. KEY POINTS: • In thraustochytrids...
Source: Applied Microbiology and Biotechnology - July 22, 2021 Category: Microbiology Authors: E-Ming Rau Inga Marie Aasen Helga Ertesv åg Source Type: research

Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production
Appl Microbiol Biotechnol. 2021 Jul 21. doi: 10.1007/s00253-021-11436-2. Online ahead of print.ABSTRACT2,3-Butanediol (2,3-BDO) is a promising commodity chemical with various industrial applications. While petroleum-based chemical processes currently dominate the industrial production of 2,3-BDO, fermentation-based production of 2,3-BDO provides an attractive alternative to chemical-based processes with regards to economic and environmental sustainability. The achievement of high 2,3-BDO titer, yield, and productivity in microbial fermentation is a prerequisite for the production of 2,3-BDO at large scales. Also, enantiopu...
Source: Applied Microbiology and Biotechnology - July 21, 2021 Category: Microbiology Authors: Jae Won Lee Ye-Gi Lee Yong-Su Jin Christopher V Rao Source Type: research

Identification of the cognate response regulator of the orphan histidine kinase OhkA involved in both secondary metabolism and morphological differentiation in Streptomyces coelicolor
Appl Microbiol Biotechnol. 2021 Jul 21. doi: 10.1007/s00253-021-11442-4. Online ahead of print.ABSTRACTIn the model actinomycete strain, Streptomyces coelicolor, an orphan histidine kinase (HK) named OhkA (encoded by SCO1596), which belongs to bacterial two-component regulatory systems (TCSs), has been identified as being involved in the regulation of both antibiotic biosynthesis and morphological development. However, its cognate response regulator (RR) remains unknown due to its isolated genetic location on the genome, which impedes the elucidation of the mechanism underlying OhkA-mediated regulation. Here, we identified...
Source: Applied Microbiology and Biotechnology - July 21, 2021 Category: Microbiology Authors: Guosong Zheng Panpan Liu Wenyan He Hengnuo Tao Zhen Yang Chuanwen Sun Wenfang Wang Yinhua Lu Weihong Jiang Source Type: research

Microbial application of thermophilic Thermoanaerobacterium species in lignocellulosic biorefinery
Appl Microbiol Biotechnol. 2021 Jul 20. doi: 10.1007/s00253-021-11450-4. Online ahead of print.ABSTRACTRecently, thermophilic Thermoanaerobacterium species have attracted increasing attentions in consolidated bioprocessing (CBP), which can directly utilize lignocellulosic materials for biofuels production. Compared to the mesophilic process, thermophilic process shows greater prospects in CBP due to its relatively highly efficiency of lignocellulose degradation. In addition, thermophilic conditions can avoid microbial contamination, reduce the cooling costs, and further facilitate the downstream product recovery. However, ...
Source: Applied Microbiology and Biotechnology - July 20, 2021 Category: Microbiology Authors: Mengdi Wu Yujia Jiang Yansong Liu Lu Mou Wenming Zhang Fengxue Xin Min Jiang Source Type: research

Metabolic engineering of Saccharomyces cerevisiae for enhanced production of caffeic acid
In this study, we first established a controllable and stable caffeic acid pathway by employing a modified GAL regulatory system to control the genome-integrated pathway genes in S. cerevisiae and realized biosynthesis of 222.7 mg/L caffeic acid. Combinatorial engineering strategies including eliminating the tyrosine-induced feedback inhibition, deleting genes involved in competing pathways, and overexpressing rate-limiting enzymes led to about 2.6-fold improvement in the caffeic acid production, reaching up to 569.0 mg/L in shake-flask cultures. To our knowledge, this is the highest ever reported titer of caffeic acid syn...
Source: Applied Microbiology and Biotechnology - July 20, 2021 Category: Microbiology Authors: Pingping Zhou Chunlei Yue Bin Shen Yi Du Nannan Xu Lidan Ye Source Type: research

Microbial application of thermophilic Thermoanaerobacterium species in lignocellulosic biorefinery
Appl Microbiol Biotechnol. 2021 Jul 20. doi: 10.1007/s00253-021-11450-4. Online ahead of print.ABSTRACTRecently, thermophilic Thermoanaerobacterium species have attracted increasing attentions in consolidated bioprocessing (CBP), which can directly utilize lignocellulosic materials for biofuels production. Compared to the mesophilic process, thermophilic process shows greater prospects in CBP due to its relatively highly efficiency of lignocellulose degradation. In addition, thermophilic conditions can avoid microbial contamination, reduce the cooling costs, and further facilitate the downstream product recovery. However, ...
Source: Applied Microbiology and Biotechnology - July 20, 2021 Category: Microbiology Authors: Mengdi Wu Yujia Jiang Yansong Liu Lu Mou Wenming Zhang Fengxue Xin Min Jiang Source Type: research

Metabolic engineering of Saccharomyces cerevisiae for enhanced production of caffeic acid
In this study, we first established a controllable and stable caffeic acid pathway by employing a modified GAL regulatory system to control the genome-integrated pathway genes in S. cerevisiae and realized biosynthesis of 222.7 mg/L caffeic acid. Combinatorial engineering strategies including eliminating the tyrosine-induced feedback inhibition, deleting genes involved in competing pathways, and overexpressing rate-limiting enzymes led to about 2.6-fold improvement in the caffeic acid production, reaching up to 569.0 mg/L in shake-flask cultures. To our knowledge, this is the highest ever reported titer of caffeic acid syn...
Source: Applied Microbiology and Biotechnology - July 20, 2021 Category: Microbiology Authors: Pingping Zhou Chunlei Yue Bin Shen Yi Du Nannan Xu Lidan Ye Source Type: research

Correction to: Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteria
Appl Microbiol Biotechnol. 2021 Jul 19. doi: 10.1007/s00253-021-11409-5. Online ahead of print.NO ABSTRACTPMID:34278512 | DOI:10.1007/s00253-021-11409-5 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 19, 2021 Category: Microbiology Authors: Lasse van Geelen Farnusch Kaschani Shabnam S Sazzadeh Emmanuel T Adeniyi Dieter Meier Peter Proksch Klaus Pfeffer Markus Kaiser Thomas R Ioerger Rainer Kalscheuer Source Type: research

Correction to: Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteria
Appl Microbiol Biotechnol. 2021 Jul 19. doi: 10.1007/s00253-021-11409-5. Online ahead of print.NO ABSTRACTPMID:34278512 | DOI:10.1007/s00253-021-11409-5 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 19, 2021 Category: Microbiology Authors: Lasse van Geelen Farnusch Kaschani Shabnam S Sazzadeh Emmanuel T Adeniyi Dieter Meier Peter Proksch Klaus Pfeffer Markus Kaiser Thomas R Ioerger Rainer Kalscheuer Source Type: research

Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain
Appl Microbiol Biotechnol. 2021 Jul 17. doi: 10.1007/s00253-021-11440-6. Online ahead of print.ABSTRACTThe expression of functional proteins on the cell surface using glycosylphosphatidylinositol (GPI)-anchoring technology is a promising approach for constructing yeast cells with special functions. The functionality of surface-engineered yeast strains strongly depends on the amount of functional proteins displayed on their cell surface. On the other hand, since the yeast cell wall space is finite, heterologous protein carrying capacity of the cell wall is limited. Here, we report the effect of CCW12 and CCW14 knockout, whi...
Source: Applied Microbiology and Biotechnology - July 17, 2021 Category: Microbiology Authors: Kentaro Inokuma Yuki Kitada Takahiro Bamba Yuma Kobayashi Takahiro Yukawa Riaan den Haan Willem Heber van Zyl Akihiko Kondo Tomohisa Hasunuma Source Type: research

Diet-induced microbiome shifts of sympatric overwintering birds
Appl Microbiol Biotechnol. 2021 Jul 17. doi: 10.1007/s00253-021-11448-y. Online ahead of print.ABSTRACTGut microbiota have a significant impact on host physiology and health, and host genetics and diet are considered as two important factors, but it is difficult to discriminate the influence of each single factor (host or diet) on gut microbiota under natural conditions. Moreover, current studies of avian microbiota mainly focus on domestic or captive birds, and it is still uncertain how host and diet take part in changing avian gut microbiota composition, diversity, and function in the wild. Here, high-throughput sequenci...
Source: Applied Microbiology and Biotechnology - July 17, 2021 Category: Microbiology Authors: Chao Li Yan Liu Minghao Gong Changming Zheng Chenglin Zhang Huixin Li Wanyu Wen Yuhang Wang Gang Liu Source Type: research

Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain
Appl Microbiol Biotechnol. 2021 Jul 17. doi: 10.1007/s00253-021-11440-6. Online ahead of print.ABSTRACTThe expression of functional proteins on the cell surface using glycosylphosphatidylinositol (GPI)-anchoring technology is a promising approach for constructing yeast cells with special functions. The functionality of surface-engineered yeast strains strongly depends on the amount of functional proteins displayed on their cell surface. On the other hand, since the yeast cell wall space is finite, heterologous protein carrying capacity of the cell wall is limited. Here, we report the effect of CCW12 and CCW14 knockout, whi...
Source: Applied Microbiology and Biotechnology - July 17, 2021 Category: Microbiology Authors: Kentaro Inokuma Yuki Kitada Takahiro Bamba Yuma Kobayashi Takahiro Yukawa Riaan den Haan Willem Heber van Zyl Akihiko Kondo Tomohisa Hasunuma Source Type: research

Diet-induced microbiome shifts of sympatric overwintering birds
Appl Microbiol Biotechnol. 2021 Jul 17. doi: 10.1007/s00253-021-11448-y. Online ahead of print.ABSTRACTGut microbiota have a significant impact on host physiology and health, and host genetics and diet are considered as two important factors, but it is difficult to discriminate the influence of each single factor (host or diet) on gut microbiota under natural conditions. Moreover, current studies of avian microbiota mainly focus on domestic or captive birds, and it is still uncertain how host and diet take part in changing avian gut microbiota composition, diversity, and function in the wild. Here, high-throughput sequenci...
Source: Applied Microbiology and Biotechnology - July 17, 2021 Category: Microbiology Authors: Chao Li Yan Liu Minghao Gong Changming Zheng Chenglin Zhang Huixin Li Wanyu Wen Yuhang Wang Gang Liu Source Type: research

D-Galacturonic acid reduction by S. cerevisiae for L-galactonate production from extracted sugar beet press pulp hydrolysate
Appl Microbiol Biotechnol. 2021 Jul 16. doi: 10.1007/s00253-021-11433-5. Online ahead of print.ABSTRACTPectin-rich residues are considered as promising feedstocks for sustainable production of platform chemicals. Enzymatic hydrolysis of extracted sugar beet press pulp (SBPP) releases the main constituent of pectin, D-galacturonic acid (D-GalA). Using engineered Saccharomyces cerevisiae, D-GalA is then reduced to L-galactonate (L-GalOA) with sorbitol as co-substrate. The current work addresses the combination of enzymatic hydrolysis of pectin in SBPP with a consecutive optimized biotransformation of the released D-GalA to L...
Source: Applied Microbiology and Biotechnology - July 16, 2021 Category: Microbiology Authors: J Wagner D Sch äfer N von den Eichen C Haimerl S Harth M Oreb J P Benz D Weuster-Botz Source Type: research

Correction to: Biotransformation of bisphenol a analogues by the biphenyl-degrading bacterium Cupriavidus basilensis - a structure-biotransformation relationship
Appl Microbiol Biotechnol. 2021 Jul 16. doi: 10.1007/s00253-021-11421-9. Online ahead of print.NO ABSTRACTPMID:34268582 | DOI:10.1007/s00253-021-11421-9 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 16, 2021 Category: Microbiology Authors: Marie-Katherin Z ühlke Rabea Schl üter Annett Mikolasch Ann-Kristin Henning Martin Giersberg Michael Lalk Gotthard Kunze Thomas Schweder Tim Urich Frieder Schauer Source Type: research

D-Galacturonic acid reduction by S. cerevisiae for L-galactonate production from extracted sugar beet press pulp hydrolysate
Appl Microbiol Biotechnol. 2021 Jul 16. doi: 10.1007/s00253-021-11433-5. Online ahead of print.ABSTRACTPectin-rich residues are considered as promising feedstocks for sustainable production of platform chemicals. Enzymatic hydrolysis of extracted sugar beet press pulp (SBPP) releases the main constituent of pectin, D-galacturonic acid (D-GalA). Using engineered Saccharomyces cerevisiae, D-GalA is then reduced to L-galactonate (L-GalOA) with sorbitol as co-substrate. The current work addresses the combination of enzymatic hydrolysis of pectin in SBPP with a consecutive optimized biotransformation of the released D-GalA to L...
Source: Applied Microbiology and Biotechnology - July 16, 2021 Category: Microbiology Authors: J Wagner D Sch äfer N von den Eichen C Haimerl S Harth M Oreb J P Benz D Weuster-Botz Source Type: research

Correction to: Biotransformation of bisphenol a analogues by the biphenyl-degrading bacterium Cupriavidus basilensis - a structure-biotransformation relationship
Appl Microbiol Biotechnol. 2021 Jul 16. doi: 10.1007/s00253-021-11421-9. Online ahead of print.NO ABSTRACTPMID:34268582 | DOI:10.1007/s00253-021-11421-9 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 16, 2021 Category: Microbiology Authors: Marie-Katherin Z ühlke Rabea Schl üter Annett Mikolasch Ann-Kristin Henning Martin Giersberg Michael Lalk Gotthard Kunze Thomas Schweder Tim Urich Frieder Schauer Source Type: research

The "beauty in the beast"-the multiple uses of Priestia megaterium in biotechnology
Appl Microbiol Biotechnol. 2021 Jul 15. doi: 10.1007/s00253-021-11424-6. Online ahead of print.ABSTRACTOver 30 years, the Gram-positive bacterium Priestia megaterium (previously known as Bacillus megaterium) was systematically developed for biotechnological applications ranging from the production of small molecules like vitamin B12, over polymers like polyhydroxybutyrate (PHB) up to the in vivo and in vitro synthesis of multiple proteins and finally whole-cell applications. Here we describe the use of the natural vitamin B12 (cobalamin) producer P. megaterium for the elucidation of the biosynthetic pathway and the subsequ...
Source: Applied Microbiology and Biotechnology - July 15, 2021 Category: Microbiology Authors: Rebekka Biedendieck Tobias Knuuti Simon J Moore Dieter Jahn Source Type: research

The "beauty in the beast"-the multiple uses of Priestia megaterium in biotechnology
Appl Microbiol Biotechnol. 2021 Jul 15. doi: 10.1007/s00253-021-11424-6. Online ahead of print.ABSTRACTOver 30 years, the Gram-positive bacterium Priestia megaterium (previously known as Bacillus megaterium) was systematically developed for biotechnological applications ranging from the production of small molecules like vitamin B12, over polymers like polyhydroxybutyrate (PHB) up to the in vivo and in vitro synthesis of multiple proteins and finally whole-cell applications. Here we describe the use of the natural vitamin B12 (cobalamin) producer P. megaterium for the elucidation of the biosynthetic pathway and the subsequ...
Source: Applied Microbiology and Biotechnology - July 15, 2021 Category: Microbiology Authors: Rebekka Biedendieck Tobias Knuuti Simon J Moore Dieter Jahn Source Type: research

Relevance of peroxiredoxins in pathogenic microorganisms
Appl Microbiol Biotechnol. 2021 Jul 14. doi: 10.1007/s00253-021-11360-5. Online ahead of print.ABSTRACTThe oxidative and nitrosative responses generated by animals and plants are important defenses against infection and establishment of pathogenic microorganisms such as bacteria, fungi, and protozoa. Among distinct oxidant species, hydroperoxides are a group of chemically diverse compounds that comprise small hydrophilic molecules, such as hydrogen peroxide and peroxynitrite, and bulky hydrophobic species, such as organic hydroperoxides. Peroxiredoxins (Prx) are ubiquitous enzymes that use a highly reactive cysteine residu...
Source: Applied Microbiology and Biotechnology - July 14, 2021 Category: Microbiology Authors: Marcos Antonio de Oliveira Carlos A Tairum Luis Eduardo Soares Netto Ana Laura Pires de Oliveira Rogerio Luis Aleixo-Silva Vitoria Isabela Montanhero Cabrera Carlos A Breyer Melina Cardoso Dos Santos Source Type: research

Relevance of peroxiredoxins in pathogenic microorganisms
Appl Microbiol Biotechnol. 2021 Jul 14. doi: 10.1007/s00253-021-11360-5. Online ahead of print.ABSTRACTThe oxidative and nitrosative responses generated by animals and plants are important defenses against infection and establishment of pathogenic microorganisms such as bacteria, fungi, and protozoa. Among distinct oxidant species, hydroperoxides are a group of chemically diverse compounds that comprise small hydrophilic molecules, such as hydrogen peroxide and peroxynitrite, and bulky hydrophobic species, such as organic hydroperoxides. Peroxiredoxins (Prx) are ubiquitous enzymes that use a highly reactive cysteine residu...
Source: Applied Microbiology and Biotechnology - July 14, 2021 Category: Microbiology Authors: Marcos Antonio de Oliveira Carlos A Tairum Luis Eduardo Soares Netto Ana Laura Pires de Oliveira Rogerio Luis Aleixo-Silva Vitoria Isabela Montanhero Cabrera Carlos A Breyer Melina Cardoso Dos Santos Source Type: research

Exploration and characterization of hypoxia-inducible endogenous promoters in Aspergillus niger
Appl Microbiol Biotechnol. 2021 Jul 12. doi: 10.1007/s00253-021-11417-5. Online ahead of print.ABSTRACTAspergillus niger is widely used for the efficient production of organic acids and enzyme preparations. However, this organism lacks basic genetic elements for dynamic control, especially inducible promoters that can respond to specific environmental signals. Since these are desirable for better adaptation of fermentation to large-scale industrial production, herein, we have identified the two first hypoxia-inducible promoters in A. niger, PsrbB and PfhbA. Their performance under high or low oxygen conditions was monitore...
Source: Applied Microbiology and Biotechnology - July 13, 2021 Category: Microbiology Authors: Xianzun Xiao Liming Ouyang Jie Qi Ju Chu Source Type: research

|Isolation and characterization of novel bacteriophages as a potential therapeutic option for Escherichia coli urinary tract infections
In conclusion, these novel phages showed the ability to decrease biofilm and bacterial adherence, making them promising candidates for effective adjuvant treatment against UTIs caused by MDR UPEC strains. KEY POINTS: Phage with lytic activity against MDR UPEC strains were isolated and characterized under in vitro conditions. A novel method was proposed to evaluate phage activity against bacterial adherence in HEp-2 cell.. Phages represent a suitable strategy to control infections caused by MDR bacteria.PMID:34254156 | DOI:10.1007/s00253-021-11432-6 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 13, 2021 Category: Microbiology Authors: Edgar Gonz ález-Villalobos Rosa Mar ía Ribas-Aparicio Gerardo Erbey Rodea Montealegre Laura Belmont-Monroy Yerisaidy Ortega-Garc ía Gerardo Aparicio-Ozores Jos é Luis Balcázar Carlos Alberto Eslava-Campos Ulises Hern ández-Chiñas Jos é Molina-Lóp Source Type: research

Biochemical and structural insights into 6-phosphogluconate dehydrogenase from Leishmania donovani
Appl Microbiol Biotechnol. 2021 Jul 12. doi: 10.1007/s00253-021-11434-4. Online ahead of print.ABSTRACT6-phosphogluconate dehydrogenase (6PGDH) participates in pentose phosphate pathway of glucose metabolism by catalyzing oxidative decarboxylation of 6-phsophogluconate (6PG) and its absence has been lethal for several eukaryotes. Despite being a validated drug target in many organisms like Plasmodium, the enzyme has not been explored in leishmanial parasites. In the present study, 6PGDH of Leishmania donovani (Ld6PGDH) is cloned and purified followed by its characterization using biochemical and structural approaches. Ld6P...
Source: Applied Microbiology and Biotechnology - July 12, 2021 Category: Microbiology Authors: Pranay Jakkula Bandigi Narsimulu Insaf Ahmed Qureshi Source Type: research

Antifungal activity of different Xenorhabdus and Photorhabdus species against various fungal phytopathogens and identification of the antifungal compounds from X. szentirmaii
Appl Microbiol Biotechnol. 2021 Jul 12. doi: 10.1007/s00253-021-11435-3. Online ahead of print.ABSTRACTXenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of Steinernema and Heterorhabditis nematodes, respectively. These bacteria produce an extensive set of natural products (NPs) with antibacterial, antifungal, antiprotozoal, insecticidal, or other bioactivities when vectored into insect hemocoel by nematodes. We assessed the in vitro activity of different Xenorhabdus and Photorhabdus cell-free supernatants against important fungal phytopathogens, viz., Cryphonectria parasitica, Fusarium oxysporum, Rhizoctoni...
Source: Applied Microbiology and Biotechnology - July 12, 2021 Category: Microbiology Authors: Harun Cimen Mustapha Touray Sebnem Hazal Gulsen Omer Erincik Sebastian L Wenski Helge B Bode David Shapiro-Ilan Selcuk Hazir Source Type: research

Biochemical and structural insights into 6-phosphogluconate dehydrogenase from Leishmania donovani
Appl Microbiol Biotechnol. 2021 Jul 12. doi: 10.1007/s00253-021-11434-4. Online ahead of print.ABSTRACT6-phosphogluconate dehydrogenase (6PGDH) participates in pentose phosphate pathway of glucose metabolism by catalyzing oxidative decarboxylation of 6-phsophogluconate (6PG) and its absence has been lethal for several eukaryotes. Despite being a validated drug target in many organisms like Plasmodium, the enzyme has not been explored in leishmanial parasites. In the present study, 6PGDH of Leishmania donovani (Ld6PGDH) is cloned and purified followed by its characterization using biochemical and structural approaches. Ld6P...
Source: Applied Microbiology and Biotechnology - July 12, 2021 Category: Microbiology Authors: Pranay Jakkula Bandigi Narsimulu Insaf Ahmed Qureshi Source Type: research

Antifungal activity of different Xenorhabdus and Photorhabdus species against various fungal phytopathogens and identification of the antifungal compounds from X. szentirmaii
Appl Microbiol Biotechnol. 2021 Jul 12. doi: 10.1007/s00253-021-11435-3. Online ahead of print.ABSTRACTXenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of Steinernema and Heterorhabditis nematodes, respectively. These bacteria produce an extensive set of natural products (NPs) with antibacterial, antifungal, antiprotozoal, insecticidal, or other bioactivities when vectored into insect hemocoel by nematodes. We assessed the in vitro activity of different Xenorhabdus and Photorhabdus cell-free supernatants against important fungal phytopathogens, viz., Cryphonectria parasitica, Fusarium oxysporum, Rhizoctoni...
Source: Applied Microbiology and Biotechnology - July 12, 2021 Category: Microbiology Authors: Harun Cimen Mustapha Touray Sebnem Hazal Gulsen Omer Erincik Sebastian L Wenski Helge B Bode David Shapiro-Ilan Selcuk Hazir Source Type: research

Production of bioactive recombinant human fibroblast growth factor 12 using a new transient expression vector in E. coli and its neuroprotective effects
In conclusion, we have established an efficient method to express and purify active rhFGF12 using an Escherichia coli expression system. Besides, rhFGF12 plays a protective effect of on nerve damage, and it provides a promising therapeutic approach for nerve injury. KEY POINTS: • Effective expression and purification of bioactive rhFGF12 protein in E. coli. • ERK/MAPK pathway is involved in rhFGF12-stimulated proliferation on PC12 cells. • The rhFGF12 has the neuroprotective effects by inhibiting apoptosis.PMID:34244814 | DOI:10.1007/s00253-021-11430-8 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 10, 2021 Category: Microbiology Authors: Mi Zhou Jiangfei Chen Kuikui Meng Yu Zhang Meng Zhang Panyu Lu Yongjun Feng Mai Huang Qiaoxiang Dong Xiaokun Li Haishan Tian Source Type: research

Production of bioactive recombinant human fibroblast growth factor 12 using a new transient expression vector in E. coli and its neuroprotective effects
In conclusion, we have established an efficient method to express and purify active rhFGF12 using an Escherichia coli expression system. Besides, rhFGF12 plays a protective effect of on nerve damage, and it provides a promising therapeutic approach for nerve injury. KEY POINTS: • Effective expression and purification of bioactive rhFGF12 protein in E. coli. • ERK/MAPK pathway is involved in rhFGF12-stimulated proliferation on PC12 cells. • The rhFGF12 has the neuroprotective effects by inhibiting apoptosis.PMID:34244814 | DOI:10.1007/s00253-021-11430-8 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - July 10, 2021 Category: Microbiology Authors: Mi Zhou Jiangfei Chen Kuikui Meng Yu Zhang Meng Zhang Panyu Lu Yongjun Feng Mai Huang Qiaoxiang Dong Xiaokun Li Haishan Tian Source Type: research

Characterization of a broad-spectrum endolysin LysSP1 encoded by a Salmonella bacteriophage
In this study, the recombinant endolysin LysSP1 encoded by Salmonella Typhimurium lytic bacteriophage SLMP1 was obtained by prokaryotic expression, and its characteristics were analyzed. Ethylenediaminetetraacetic acid (EDTA) can be used as the outer membrane permeabilizer to increase the bactericidal activity of LysSP1. Under the synergism of 5 mmol/L EDTA, LysSP1 exhibited a strong bactericidal activity against Salmonella Typhimurium ATCC14028. LysSP1 was stable at 4°C for 7 days and at -20°C for 180 days. LysSP1 remained the optimal activity at 40°C and was efficiently active at alkaline condition (pH 8.0-10...
Source: Applied Microbiology and Biotechnology - July 9, 2021 Category: Microbiology Authors: Yanhua Jiang Dongqin Xu Lianzhu Wang Meng Qu Fengling Li Zhijun Tan Lin Yao Source Type: research

The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger
In this study, we used the CRISPR/Cas9 system to generate three chimeric GaaR-XlnR transcription factors expressed by the xlnR promoter by swapping the N-terminal region of the xylanolytic regulator XlnR to that of the GaaR in A. niger. As a test case, we constructed a PpgaX-hph reporter strain to evaluate the alteration of transcription factor specificity in the chimeric mutants. Our results showed that the chimeric GaaR-XlnR transcription factor was induced in the presence of D-xylose. Additionally, we generated a constitutively active GaaR-XlnR V756F version of the most efficient chimeric transcription factor to better ...
Source: Applied Microbiology and Biotechnology - July 8, 2021 Category: Microbiology Authors: Roland S Kun Sandra Garrigues Marcos Di Falco Adrian Tsang Ronald P de Vries Source Type: research

Bio-prospecting the future in perspective of amidohydrolase L-glutaminase from marine habitats
Appl Microbiol Biotechnol. 2021 Jul 8. doi: 10.1007/s00253-021-11416-6. Online ahead of print.ABSTRACTIn the current scenario, considerable attention is being given to the enzyme L-glutaminase (EC 3.5.1.2). It belongs to the amidohydrolase class adherent to the family of serine-reliant β-lactamases and the penicillin-binding proteins due to its higher affinity to polymerize and modify peptidoglycan synthesis. However, based on the catalytic proficiency, L-glutaminase is characterized as a proteolytic endopeptidase that cleaves peptide linkage and emancipates various byproducts, viz. ammonia along with glutamate. L-glu...
Source: Applied Microbiology and Biotechnology - July 8, 2021 Category: Microbiology Authors: Nidhi Y Patel Dhritiksha M Baria Shivani M Yagnik Kiransinh N Rajput Rakesh R Panchal Vikram H Raval Source Type: research

The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger
In this study, we used the CRISPR/Cas9 system to generate three chimeric GaaR-XlnR transcription factors expressed by the xlnR promoter by swapping the N-terminal region of the xylanolytic regulator XlnR to that of the GaaR in A. niger. As a test case, we constructed a PpgaX-hph reporter strain to evaluate the alteration of transcription factor specificity in the chimeric mutants. Our results showed that the chimeric GaaR-XlnR transcription factor was induced in the presence of D-xylose. Additionally, we generated a constitutively active GaaR-XlnR V756F version of the most efficient chimeric transcription factor to better ...
Source: Applied Microbiology and Biotechnology - July 8, 2021 Category: Microbiology Authors: Roland S Kun Sandra Garrigues Marcos Di Falco Adrian Tsang Ronald P de Vries Source Type: research

Bio-prospecting the future in perspective of amidohydrolase L-glutaminase from marine habitats
Appl Microbiol Biotechnol. 2021 Jul 8. doi: 10.1007/s00253-021-11416-6. Online ahead of print.ABSTRACTIn the current scenario, considerable attention is being given to the enzyme L-glutaminase (EC 3.5.1.2). It belongs to the amidohydrolase class adherent to the family of serine-reliant β-lactamases and the penicillin-binding proteins due to its higher affinity to polymerize and modify peptidoglycan synthesis. However, based on the catalytic proficiency, L-glutaminase is characterized as a proteolytic endopeptidase that cleaves peptide linkage and emancipates various byproducts, viz. ammonia along with glutamate. L-glu...
Source: Applied Microbiology and Biotechnology - July 8, 2021 Category: Microbiology Authors: Nidhi Y Patel Dhritiksha M Baria Shivani M Yagnik Kiransinh N Rajput Rakesh R Panchal Vikram H Raval Source Type: research