Rapid antimicrobial susceptibility testing and β-lactam-induced cell morphology changes of Gram-negative biological threat pathogens by optical screening

For Yersinia pestis, Burkholderia pseudomallei, and Burkholderia mallei, conventional broth microdilution (BMD) is considered the gold standard for antimicrobial susceptibility testing (AST) and, depending on the...
Source: BMC Microbiology - Category: Microbiology Authors: Tags: Research article Source Type: research

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This study aims at detection of R. typhi in rodent fleas by conventional polymerase chain reaction (PCR). A cross sectional study ...
Source: African Journal of Microbiology Research - Category: Microbiology Source Type: research
Abstract Immunochromatography assays are simple and low-cost point-of-care diagnostics. However, the dependence on paper-based capillary forces limits improvements in speed of the assay. An electro-driven immunochromatography assay (EICA) is described that uses electroosmotic flow (EOF) to increase the driving force, as well as upconversion nanoparticles for high-sensitivity detection. EOF increased the signal intensity by 64.0% and reduced the assay time from 15 min to 5 min, and the EICA exhibited good specificity and sensitivity (1.2 × 103 CFU/mL) for pure cultures of Yersinia pes...
Source: Biosensors and Bioelectronics - Category: Biotechnology Authors: Tags: Biosens Bioelectron Source Type: research
This article has reviewed the lowest molecular mass for L-asparaginase in Yersinia pseudotuberculosis Q66CJ2 which is 36.27 kDa, while the highest for Pseudomonas otitidis which has a molecular mass of 205 ± 3 kDa. This review is an attempt to summarize most of the available sources, their phylogenetic relationships, purification methods, data regarding different physiochemical and kinetic properties of L-asparaginase. PMID: 32052094 [PubMed - as supplied by publisher]
Source: Archives of Microbiology - Category: Microbiology Authors: Tags: Arch Microbiol Source Type: research
P. Rongkard et al.
Source: Emerging Infectious Diseases Journal - Category: Infectious Diseases Source Type: research
Publication date: Available online 8 February 2020Source: Veterinary MicrobiologyAuthor(s): Priyangi A Alwis, Puthayalai Treerat, Lan Gong, Deanna Deveson Lucas, Elizabeth M Allwood, Mark Prescott, Rodney J Devenish, Ben Adler, John D BoyceAbstractTwo-component signal transduction systems (TCSTS) are abundant among prokaryotes and regulate important functions, including drug resistance and virulence. The Gram-negative bacterium Burkholderia pseudomallei, which causes the severe infectious disease melioidosis, encodes 136 putative TCSTS components. In silico analyses of these TCSTS indicated that the predicted BbeR-BbeS sys...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Abstract A spider-transmitted fungus ( Rhizopus microsporus ) that was isolated from necrotic human tissue was found to harbor endofungal bacteria ( Burkholderia sp.). Metabolic profiling of the symbionts revealed a complex of cytotoxic agents (necroximes). Their structures were fully characterized as oxime-substituted benzolactone enamides equipped with a peptidic side chain. Necroximes are potent cytotoxic agents that are also formed in symbiosis with the fungal host and could have contributed to the necrosis. Genome sequencing and in silico analyses revealed a novel modular PKS/NRPS assembly line equipped with ...
Source: Angewandte Chemie - Category: Chemistry Authors: Tags: Angew Chem Int Ed Engl Source Type: research
In this study, the therapeutic potential of combinations of an antibiotic and a broad-spectrum antimicrobial peptide (AMP) was evaluated against five bacterial biothreats, the etiologic agents of glanders (Burkholderia mallei), melioidosis (Burkholderia pseudomallei), plague (Yersinia pestis), tularemia (Francisella tularensis), and anthrax (Bacillus anthracis). The therapeutics included licensed early generation antibiotics which are now rarely used. Three antibiotics and one 24- amino acid AMP were selected based on MIC assay data. Combinations of the AMP and tigecycline, minocycline, or novobiocin were screened for syne...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Abstract To improve the preparedness against exposure to highly pathogenic bacteria and to anticipate the wide variety of bacteria that can cause bloodstream infections (BSIs), a safe, unbiased and highly accurate identification method was developed. Our liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method can identify highly pathogenic bacteria, their near-neighbors and bacteria that are common causes of BSIs directly from positive blood culture flasks. The developed Peptide-Based Microbe Detection Engine (http://proteome2pathogen.com) relies on a two-step workflow: a genus-level search followed...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research
Abstract Burkholderia pseudomallei (B. pseudomallei), the etiological agent of melioidosis, is a Gram-negative bacterium with additional concern as a biothreat pathogen. The mortality rate from B. pseudomallei varies depending on the type of infection and extent of available health care, but in the case of septicemia left untreated it can range from 50 - 90%. Current therapy for melioidosis is biphasic, consisting of parenteral acute-phase treatment for two weeks or longer, followed by oral eradication-phase treatment lasting several months. An effective oral therapeutic for outpatient treatment of acute-phase mel...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-plate formic acid, and ethanol/formic acid tube extraction methods, were evaluated for their ability to render highly pathogenic organisms nonviable and safe for handling in a biosafety level 2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight-laboratory study to examine the u...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research
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