Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms Bacteriology

Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-plate formic acid, and ethanol/formic acid tube extraction methods, were evaluated for their ability to render highly pathogenic organisms nonviable and safe for handling in a biosafety level 2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight-laboratory study to examine the utility of the Bruker Biotyper and Vitek MS MALDI-TOF MS systems and their in vitro diagnostic (IVD), research-use-only, and Security-Relevant databases, as applicable, to accurately identify these agents. Forty-six distinct strains of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, Burkholderia pseudomallei, Clostridium botulinum, Brucella melitensis, Brucella abortus, Brucella suis, and Brucella canis were extracted and distributed to participating laboratories for analysis. A total of 35 near-neighbor isolates were also analyzed.
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research

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In human brucellosis, the liver is frequently affected. Brucella abortus triggers a profibrotic response on hepatic stellate cells (HSCs) characterized by inhibition of MMP-9 with concomitant collagen deposition and TGF-β1 secretion through type 4 secretion system (T4SS). Taking into account that it has been reported that the inflammasome is necessary to induce a fibrotic phenotype in HSC, we hypothesized that Brucella infection might create a microenvironment that would promote inflammasome activation with concomitant profibrogenic phenotype in HSCs. B. abortus infection induces IL-1β secretion in HSCs in a T4SS...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Brucellosis, caused by intracellular gram-negative pathogens of the genus Brucella, continues to be one of the most pandemic zoonotic diseases in most countries. At present, the therapeutic treatment of brucellosis relies on a combination of multiple antibiotics that involves a long course of treatment, easy relapse, and high side effects from the use of certain antibiotics (such as streptomycin). Thus, the need to identify novel drugs or targets to control this disease is urgent. Diaminopimelate decarboxylase (DAPDC), a key enzyme involved in the bacterial diaminopimelate (DAP) biosynthetic pathway, was suggested to be a ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
[The Herald] EIGHTY-SEVEN people have been hospitalised after eating meat from cattle affected by anthrax.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
Publication date: Available online 19 January 2020Source: Biosensors and BioelectronicsAuthor(s): Yong Zhao, Pingping Zhang, Jin Wang, Lei Zhou, Ruifu YangAbstractImmunochromatography assays are simple and low-cost point-of-care diagnostics. However, the dependence on paper-based capillary forces limits improvements in speed of the assay. An electro-driven immunochromatography assay (EICA) is described that uses electroosmotic flow (EOF) to increase the driving force, as well as upconversion nanoparticles for high-sensitivity detection. EOF increased the signal intensity by 64.0% and reduced the assay time from 15 min to...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research
AbstractBrucellosis is a zoonosis mainly present in developing countries. The WHO reports 500,000 new cases every year. From 2012 to 2016, 13,677 cases were reported in Mexico, with 2.00 to 2.64 rate per 100,000 inhabitants. To analyze the diagnostic algorithm of brucellosis in Mexico, we compared the commercial laboratory tests ELISA, Brucellacapt ®, and lateral flow test (LFT) in a study of 473 individuals from two endemic Mexican populations. All patients were treated in first-level medical units for presenting brucellosis compatible symptoms and without a history of the disease. Clinical-epidemiological information...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
[New Zimbabwe] Masvingo -At least 64 people have been hospitalised in Bikita and Gutu after eating anthrax contaminated meat with 47 cattle dying from the outbreak while government has also moved in to vaccinate 22 000 herd of cattle.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
Publication date: Available online 16 January 2020Source: Fungal Biology ReviewsAuthor(s): Paola Bonfante, Francesco VeniceAbstractMany fungi (from micro-to macromycetes) interact with plants as a relevant component of plant microbiota. The aim of the review is to focus on the early diverging fungi (Mucoromycota) whose members establish a wide range of beneficial or pathogenic interactions with their green hosts, depending on their phylogenetic position. While Mortierellomycotina are mostly identified as rhizospheric microbes, Glomeromycotina are acknowledged as the most widespread arbuscular mycorrhizal fungi, leading to ...
Source: Fungal Biology Reviews - Category: Biology Source Type: research
[263Chat] The government has availed vaccines for the treatment of cattle from anthrax hit areas amid reports that 33 people have been diagnosed and hospitalized of the deadly disease in the past weeks.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
[The Herald] Masvingo -The number of people diagnosed with anthrax which hit Bikita last week has risen from 28 to 33 while cattle deaths rose to 40 from 24, an official has confirmed.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
Abstract To improve the preparedness against exposure to highly pathogenic bacteria and to anticipate the wide variety of bacteria that can cause bloodstream infections (BSIs), a safe, unbiased and highly accurate identification method was developed. Our liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method can identify highly pathogenic bacteria, their near-neighbors and bacteria that are common causes of BSIs directly from positive blood culture flasks. The developed Peptide-Based Microbe Detection Engine ( relies on a two-step workflow: a genus-level search followed...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research
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