Biogenic Synthesis of Silver Nanoparticles Using Phyllanthus emblica Fruit Extract and Its Inhibitory Action Against the Pathogen Acidovorax oryzae Strain RS-2 of Rice Bacterial Brown Stripe

In this study, we report low-cost, green synthesis of AgNPs using fresh fruit extract of Phyllanthus emblica. The biosynthesized AgNPs was confirmed and characterized by analysis of spectroscopy profile of the UV-visible and Energy dispersive spectrophotometer, Fourier transform infrared, X-ray diffraction pattern, and electron microscopy images examination. UV-visible spectra showed a surface resonance peak of 430 nm corresponding to the formation of AgNPs, and FTIR spectra confirmed the involvement of biological molecules in AgNPs synthesis. In spherical AgNPs, the particle size ranged from 19.8 to 92.8 nm and the average diameter was 39 nm. Synthesized nanoparticles at 20 μg/ml showed remarkable antimicrobial activity in vitro against the pathogen Acidovorax oryzae strain RS-2 of rice bacterial brown stripe, while 62.41% reduction in OD600 value was observed compared to the control. Moreover, the inhibitory efficiency of AgNPs increased with the increase of incubation time. Furthermore, AgNPs not only disturbed biofilm formation and swarming ability but also increased the secretion of effector Hcp in strain RS-2, resulting from damage to the cell membrane, which was substantiated by TEM images and live/dead cell staining result. Overall, this study suggested that AgNPs can be an attractive and eco-friendly candidate to control rice bacterial disease. Introduction In recent decades, the development of new and effective antimicrobials against infections caused by ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 18 January 2020Source: Clinica Chimica ActaAuthor(s): Guodong Zhao, Yong Ma, Hui Li, Shiming Li, Yun Zhu, Xiaoyu Liu, Shangmin Xiong, Yi Liu, Jin Miao, Sujuan Fei, Minxue Zheng, Xiangwei ZhaoAbstractBackgroundMethylated SFRP2 was previously reported as a non-invasive biomarker for colorectal cancer (CRC) detection with a relatively low sensitivity for early stage CRC. The purpose of this study was to evaluate the performance of a new plasma based CRC screening assay, SpecColon test, which tested methylated SFRP2 and SDC2 simultaneously in a single qPCR reaction, in detecting CRC and advan...
Source: Clinica Chimica Acta - Category: Laboratory Medicine Source Type: research
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Source: Applied Developmental Science - Category: Child Development Authors: Source Type: research
Publication date: Available online 16 January 2020Source: Food ChemistryAuthor(s): María del Mar Contreras, Antonio Lama-Muñoz, Francisco Espínola, Manuel Moya, Inmaculada Romero, Eulogio CastroAbstractOlive leaves farmed from trees are valuable for the production of functional extracts. Nonetheless, olive leaves (containing thin branches), which are separated during olives cleaning in the mill, have received little attention. In this context, a multiple response optimization was performed to maximize at once the yield, total phenolic content, oleouropein and antioxidant activity obtained by ultrasound...
Source: Food Chemistry - Category: Food Science Source Type: research
Conclusions: Although all iron treatments analyzed are effective and safe, our results suggest that iron protein succinylate may be an excellent choice to treat iron deficiency and anemia due to its superior effectiveness and tolerability. PMID: 31944128 [PubMed - as supplied by publisher]
Source: Current Medical Research and Opinion - Category: Research Tags: Curr Med Res Opin Source Type: research
Conclusion: Advanced RCC has high morbidity and mortality with an increasing prevalence. Following tyrosine kinase inhibitors, checkpoint inhibitors have a great influence on treatment of advanced RCC, especially the combination of these two strategies. In 2019 these combined strategies demonstrated 5% complete remission with up to 60% objective response rate. While not immediately, but perhaps in the near future, advanced RCC will become a manageable chronic disease, even if a cure is not possible. PMID: 31942809 [PubMed - as supplied by publisher]
Source: Current Medical Research and Opinion - Category: Research Tags: Curr Med Res Opin Source Type: research
Authors: Oikonomou EK PMID: 31944137 [PubMed - as supplied by publisher]
Source: Expert Review of Molecular Diagnostics - Category: Laboratory Medicine Tags: Expert Rev Mol Diagn Source Type: research
Publication date: Available online 16 January 2020Source: Biosensors and BioelectronicsAuthor(s): Parvin Samadi Pakchin, Marziyeh Fathi, Hossein Ghanbari, Reza Saber, Yadollah OmidiAbstractIn the current study, we report on the design and development of a novel electrochemical immunosensor for the detection of cancer antigen 125 (CA125) oncomarker. Polyamidoamine/gold nanoparticles (PAMAM/AuNPs) were used to increase the conductivity and enhance the number of antibodies (Ab) immobilized on the electrode surface. Three-dimensional reduced graphene oxide-multiwall carbon nanotubes (3DrGO-MWCNTs) were used to modify the glass...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research
Publication date: 15 April 2020Source: Journal of Molecular Structure, Volume 1206Author(s): Yaşar Gök, Halil Zeki GökAbstractTwo zinc(II) phthalocyanines (4 and 6) and two cobalt(II) phthalocyanines (5 and 7) with four (1R,2R)-1,2-bis(3ʹ-phenylphenyl)ethane-1,2-diol at peripheral and non-peripheral positions of the phthalocyanine ring have been synthesized starting from (1R,2R)-1,2-bis(3ʹ-phenylphenyl)ethane-1,2-diol substituted chiral phthalonitriles 2 and 3, respectively. The aggregation properties of newly synthesized phthalocyanines 4–7 were examined in coordinating and non-coordinating solvents by ...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: 15 April 2020Source: Journal of Molecular Structure, Volume 1206Author(s): Aisha SM. HossanAbstractThe reactions for 5-arylazo-2-chloroacetamido-4-methylthiazole with various 2-mercaptobenzothiazole and malononitrile were afforded the corresponding thiobenzothiazolyl and amino-pyrrolidinone derivatives, respectively. The prepared 4-methyl-5-(phenylarylazo)thiazol-2-yl derivatives have been elucidated by spectral performances (FT-IR, 1HNMR and MS) and elemental analyses. The synthesized compounds were subjected to quantum chemical calculations at DFT/B3LYB level to shed light on their electronic and chemic...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: 15 April 2020Source: Journal of Molecular Structure, Volume 1206Author(s): Seyed Reza Nokhbeh, Mostafa Gholizadeh, Alireza Salimi, Hazel A. SparkesAbstractThis paper presents synthesis and structural characterization of new members of phosphorus-based organic bromides. 1,3-Propanediylbis(triphenylphosphonium) dibromide I and 1,3-propanediylbis(triphenylphosphonium) monotribromide II, as a new brominating agent for double bonds and phenolic rings, were synthesized. 1H NMR, 13C NMR, 31P NMR, FT-IR, single crystal X-ray diffraction crystallography, differential scanning calorimetry, thermogravimetric analysi...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
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