Biogenic Synthesis of Silver Nanoparticles Using Phyllanthus emblica Fruit Extract and Its Inhibitory Action Against the Pathogen Acidovorax oryzae Strain RS-2 of Rice Bacterial Brown Stripe

Conclusion In conclusion, this study clearly provides an economical, environmental friendly, and straightforward reproducible approach in AgNPs synthesis employing P. Emblica fruit extracts as a reducing, stabilizing, and capping agent. The biosynthesized AgNPs were characterized thoroughly by UV-Visible and Energy Dispersive X-Ray Spectroscopy, X-Ray diffraction, FTIR, TEM, HRTEM and SEM. The FTIR results found several phytochemicals responsible for the rapid reduction of ions, leading to AgNPs formation. Especially, hydroxyl groups oxidation of hydrolysate, which likely stimulated the formation of nanoparticles. In the reaction mixture, biosynthesized AgNPs have been detected as mono-dispersed, rather stable, of comparatively smaller in shape and were adhered with an organic layer, in which proteins participated. Furthermore, this study clearly demonstrated that bacterial growths of Ao strain RS-2 were inhibited by the synthesized AgNPs, while effects varied with the period of incubation and applied concentration. Hence, it is noticeably observed that the MIC of AgNPs at 20 μg/ml was able to affect bacterial growth, cell survival, biofilm formation, and swarming ability. ELISA experiment demonstrated that AgNPs resulted in increased secretion of Hcp proteins in Ao strain RS-2, which might be justified by the damaged membrane in reference to TEM images and results of live/dead cell staining assays. The bacteriostatic effect of AgNPs is generally achieved due to direc...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research