The effect of Hepatitis D co-infection on the immunologic and molecular profile of Hepatitis B in asymptomatic Chronic Hepatitis B patients in southwest Nigeria.
Conclusion: The prevalence of HDV-Ag among asymptomatic treatment naïve chronic hepatitis B patients in Abeokuta was 11% and there was no significant difference in the levels of HBV DNA and HBsAg among those with or without hepatitis D. PMID: 32096684 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - February 27, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Pathology and immunohistochemical evaluation of lungs of cattle slaughtered at metropolitan abattoirs in Nigeria and Ghana.
Authors: Ola OO, Adeniran GA, Opoku-Agyemang T, Bediako E, Ishola O, Jarikre TA, Emikpe BO Abstract There was a dearth of information on pathology and causal agents of bovine pneumonia in West Africa. This cross-sectional study conducted at four major metropolitan abattoirs in Nigeria and Ghana was to evaluate the pathology and to immunohistochemically demonstrates viral and bacterial pathogens of bovine pneumonia in West Africa. Out of the 20,605 cattle lungs examined at post-mortem using standard inspection procedures, 136 samples grossly showed pneumonic lesions and 99 randomly selected lung samples were fixed i...
Source: Journal of Immunoassay and Immunochemistry - February 25, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Moroccan lymphocyte subsets reference ranges: age, gender, ethnicity, and socio-economic factors dependent differences.
This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. These results can be used for other North African (Maghrebian) countries considering their geographic, ethnic, economic, and cultural similarities. PMID: 32065027 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - February 20, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Biomarkers of inflammation and oxidative stress in ophthalmic disorders.
Authors: Ahmad A, Ahsan H Abstract The review article focuses on free radicals and oxidative stress involved in ophthalmological diseases such as retinopathy, cataract, glaucoma, etc. Oxidative stress is considered as a key factor involved in the pathology of many chronic diseases including ophthalmic complication and inflammatory process. Oxidative stress and inflammation are closely related pathophysiological processes and are simultaneously found in many pathological conditions. The free radicals produced oxidize cellular components such as lipids and phospholipids leading to lipid peroxidation and trigger the o...
Source: Journal of Immunoassay and Immunochemistry - February 14, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serological evidence of Coxiella burnetii infection in slaughtered sheep and goats at Kumasi Abattoir, Ghana.
This study was therefore conducted to provide evidence of exposure of sheep and goats slaughtered at the Kumasi Abattoir to Coxiella burnetii. A total of 350 serum samples collected from 175 sheep and 175 goats were analyzed for the presence of C. burnetii antibodies using a commercial ELISA kit (ID Vet). Results of the study established a seroprevalence of 28.57% in goats, 16.57% in sheep and an overall seroprevalence of 22.29% in sheep and goats; 20.57% for male sheep, 23.86% for female sheep, 26.44% for male goats and 30.68% for female goats. Results showed that goats are more at risk to the infection than sheep however...
Source: Journal of Immunoassay and Immunochemistry - February 8, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

HIV coinfections with tuberculosis among HIV-1 infected individuals in old cross river state, Nigeria.
This study evaluated the HIV/TB co-infection rate among HIV-1 infected individuals in old Cross River State, Nigeria. A total of 417 HIV-infected individuals participated in this study, 241 (57.8%) from Calabar, Cross River State, Nigeria and 176 (42.2%) from Uyo, Akwa-Ibom State, Nigeria. The age range of the 417 HIV-1 positive individuals who participated in the study was 4-72 years with an average age of 39.1 years. Plasma samples were analyzed for HIV and TB using fourth-generation Enzyme-Linked immunosorbent Assay. The CD4 count was enumerated using the Partec CyFlow® Counter. Plasma viral loads (PVL) we...
Source: Journal of Immunoassay and Immunochemistry - January 22, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Detection of Hepatitis C virus and the risk of transmission among pregnant and nursing mothers from rural and urban communities in Kogi State, Nigeria.
Authors: Ifeorah IM, Bakarey AS, Akubo AO, Onyemelukwe FN Abstract Hepatitis C virus (HCV) is associated with liver complicated diseases resulting in end-stage hepatocellular carcinoma. Although vertical transmission from mother to child serves as one of the routes of HCV acquisition in children, yet HCV infection in pregnant women and children is still underappreciated in sub-Saharan Africa. Therefore, this study investigated the burden of HCV, associated risk factors, and viremia among antenatal and postnatal clinic attendees in the rural and urban communities of Kogi State, Nigeria. Atotal of 176 blood samples w...
Source: Journal of Immunoassay and Immunochemistry - January 22, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunoglobulin G amplifies the production of regulatory rheumatoid factor in vitro through idiotype-anti-idiotype interactions.
Authors: Beduleva L, Stolyarova E, Sidorov A, Khramova T, Terentiev A, Menshikov I, Abisheva N, Snigiryev A, Fomina K Abstract Immunoglobulin G can inhibit antibody response. The mechanism of immunosuppression by immunoglobulins remains unknown. Recently, we found a new factor of immunoregulation referred to as regulatory rheumatoid factor (regRF). RegRF prevents autoimmunity and reduces experimental autoimmune reactions. RegRF comprises a population of anti-idiotypic antibodies that have a unique paratope specific to the antigen-binding sites of the antibodies, and a shared paratope specific to neoepitopes of IgG ...
Source: Journal of Immunoassay and Immunochemistry - January 15, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of in-house RIA kit for progesterone in cow's skim milk.
Authors: Benabdelaziz A, Boudjemai S, Khelili R, Besbaci M, Kaidi R Abstract A radioimmunoassay (RIA) method for progesterone has been developed. It has been validated for the determination of progesterone in cow skim milk. The main reagents used in the development work were prepared and characterized in-house. The assay uses a radioiodinated tracer purified by gel filtration on Sephadex G-25, standards prepared in skim milk and coated tubes with specific antibodies as the solid phase (separation system). The radiochemical purity of the tracer was greater than 95%, the maximum binding using solid phase reached 43% ...
Source: Journal of Immunoassay and Immunochemistry - January 1, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunological and epidemiological evaluation of EBV infections among HIV-1 infected individuals in Abakaliki, Nigeria supports the potential use of neutrophils as a marker of EBV in HIV disease progression and as useful markers of immune activation.
Authors: Okonko IO, Makinde TS, Okonko BJ, Ogbu O Abstract Human herpesvirus 4 which is commonly known as Epstein-Barr virus (EBV) is one of the opportunistic pathogens that affect human immunodeficiency virus (HIV) infected individuals and it is the leading cause of death and cancer in humans. The study was designed to evaluate the prevalence of EBV among HIV-1 infected individuals in Abakaliki metropolis, Ebonyi State, Nigeria. A total of 91 HIV-1 infected individuals were collected from Mile 4 Hospital, Abakaliki, Ebonyi State, Nigeria. ELISA was used to determine the Epstein-Barr Nuclear Antigen (EBNA) IgG and ...
Source: Journal of Immunoassay and Immunochemistry - January 1, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Determination of antibodies to human immunodeficiency virus type 1 & 2 & O and P24 - antigen in pregnant women in port harcourt Nigeria.
Determination of antibodies to human immunodeficiency virus type 1&2&O and P24 - antigen in pregnant women in port harcourt Nigeria. J Immunoassay Immunochem. 2019 Dec 28;:1-11 Authors: Okonko IO, Okoli EM Abstract The need for a cure against HIV infection and a need to improve HIV screening amongst low risk population such as pregnant women have been an issue since the emergence of HIV. Therefore, a hospital-based cross-sectional study was adopted to determine the prevalence of antibodies to HIV-1/2/O and P24 antigen among pregnant women in Port Harcourt, Nigeria. Estimating the HIV seropositi...
Source: Journal of Immunoassay and Immunochemistry - January 1, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Viral hepatitis B and C co-infection with Human Immunodeficiency Virus among adult patients attending selected highly active anti-retroviral therapy clinics in Nigeria's capital.
This study determined the seroprevalence of HIV co-infection with the two viruses among patients attending three major hospitals in the Federal Capital Territory, Nigeria. From February to July 2019, 311 sera samples were collected from HIV positive patients and screened for Hepatitis B and C infection. Immunochromatographic and ELISA techniques for HBsAg and HCV were used. Socio-demographic features and responses to risk factors were obtained using questionnaires. Patients' data and results obtained were analyzed with SPSS version 25. The prevalence of HIV/HBV/HCV, HIV/HBV, and HIV/HCV co-infection were 0.64%, 6.43%, and ...
Source: Journal of Immunoassay and Immunochemistry - December 29, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Platelet to lymphocyte ratio (PLR) in alcohol use disorder.
In this study, we compared complete blood count values of 31 AUD male patients and 31 healthy male controls. There was no significant difference between the two groups in terms of PLR (p = .123). When the age was controlled, there was a negative correlation between the duration of alcohol use and PLR (r = -0.567; p = .005). The significance of the parameters in the AUD group was found to be related to the duration of alcohol use. PMID: 31842683 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - December 19, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Comparison of two therapeutic approaches for the management of ventilator-associated pneumonia due to multidrug-resistant Acinetobacter: a randomized clinical trial study.
Authors: Khorvash F, Yaghoubi S, Farsaei S, Ataei B, Hakamifard A, Mohajeri F, Gudarzi M Abstract Management of ventilator-associated pneumonia (VAP) is a puzzling issue for infectious disease specialist. The present clinical trial study was aimed to comparing the effects of injectable colistin plus nebulized colistin and injectable colistin plus nebulized tobramycin on management of patients with VAP due to multidrug-resistant Acinetobacter. VAP patients were randomly divided into two groups (n = 30/each): Group 1 - patients that received intravenous (IV) meropenem, injectable colistin plus nebulized colistin...
Source: Journal of Immunoassay and Immunochemistry - November 30, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Semi-solid phase assay for the alternative complement pathway activity assessment (AP100).
Authors: Kerboua KE, Djenouhat K Abstract Since the introduction of the most expensive drug in the world (Eculizumab) in the therapeutic arsenal of many diseases involving the alternative complement pathway (ACP) in their pathophysiology, the unmet need to perform simple ACP assays affordable for all countries has become one of the major challenges of the contemporary medicine. The assay currently used is AH50, despite it still challenging for several laboratories. This educational chapter consists of a detail protocol of standardized hemolytic assay AP100 and aims to help clinical laboratories over the world and e...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Phylo-diversity of prevalent human E. coli O157:H7 with strains from retailed meat and fish in selected markets in Ibadan Nigeria.
Authors: Ayodele OA, Deji-Agboola AM, Akinduti PA, Faneye AO Abstract Global prevalence of ESBL-biotypes poses a serious threat to public health as a result of severity and morbidity caused by beta-lactam encoded Escherichia coli. Therefore, the prevalent shiga toxigenic Escherichia coli of ESBL variant was investigated in various retailed food animals and cooking materials. A total of 823 samples consisting of raw meat (297) and fish (132) samples retailed at various major markets in Ibadan were collected and 394 swabs were taken from the butchers' processing tables and utensils used in retailing meat and fish. Th...
Source: Journal of Immunoassay and Immunochemistry - November 27, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Complementology's foundation: The 100-year anniversary of the Nobel Prize to Jules Bordet.
Authors: Kerboua KE, Djenouhat K Abstract The discovery of the complement system was associated with the creation of medical serodiagnosis in the early 20th century. Its biotechnological applications, usable even a century after its development by Jules Bordet, preceded for decades the proof of its biochemical rather than biophysical nature. Complement science has begun to emerge, thanks to the labs of Michael Heidelberger and his student Manfred Martin Mayer. Complementology had known difficult moments like the suicide of Louis Pillemer by swallowing the reagents of his laboratory following the criticisms of his d...
Source: Journal of Immunoassay and Immunochemistry - November 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Study of the complement activation by amyloid aggregates of smooth muscle titin in vitro.
Authors: Yakupova EI, Bobylev AG, Bobyleva LG, Vikhlyantsev IM Abstract The giant muscle protein, titin, is the third most abundant protein in muscle (after myosin and actin). It was shown previously that smooth muscle titin (SMT) with a molecular mass of 500 kDa can form in vitro amorphous amyloid aggregates in two conditions: in solution of low ionic strength (0.15 M Glycine-KOH, pH 7.0) (SMT(Gly) aggregates) and in solution with ionic strength in the physiological range (0.2 M KCl, 20 mM imidazole, pH 7.2-7.4) (SMT(KCl) aggregates). Such aggregation in vivo, which may play a pathological or functional role, is n...
Source: Journal of Immunoassay and Immunochemistry - November 22, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Hepatoprotective effect of artichoke leaf extracts in comparison with silymarin on acetaminophen-induced hepatotoxicity in mice.
Authors: Elsayed Elgarawany G, Abdou AG, Maher Taie D, Motawea SM Abstract Acetaminophen is a common analgesic-antipyretic agent, which is safe in therapeutic doses but in higher doses can produce hepatic necrosis. The aim of this study is to investigate the hepatoprotective effects of artichoke, silymarin, and both agents in acetaminophen-induced liver damage in mice. Forty male mice were divided into five main groups, (1) control (2) Acetaminophen (APAP) (3) Artichoke leaf extracts (ALE) and APAP (4) silymarin and APAP group (5) ALE, silymarin and APAP groups. Blood samples were collected for the measurement of l...
Source: Journal of Immunoassay and Immunochemistry - November 20, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

An update on investigations of autoimmune diseases affecting orofacial region.
This article aims to list out the diagnostic methods to diagnose autoimmune conditions and focuses on various diagnostic methods to effectively evaluate autoimmune disease of orofacial region. PMID: 31645179 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - October 26, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroprevalence and trend of hepatitides among blood donors in a district hospital in Ghana: a nine-year retrospective, descriptive cross-sectional study.
This study aimed to determine the prevalence and trend of hepatitis infections among voluntary blood donors at Nkenkaasu District Hospital. A retrospective study of voluntary blood donors' records from January 2010 to December 2018 was conducted. Data on the donors' age, sex and serum HBsAg and Hepatitis C virus results were retrieved from the Laboratory's register and analyzed with SPSS version 16.0. Out of the 3306 total donors, the seroprevalence of hepatitis C infection was 11.7% and hepatitis B infection was 10.3%. Only 1.4% of the donors were co-infected with both pathogens and 82.7% had no infection. Rhesus blood gr...
Source: Journal of Immunoassay and Immunochemistry - October 24, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Effects of fructooligosaccharides (FOS) on growth, survival, gut microflora, stress, and immune response in Pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system.
Authors: Mustafa A, Buentello A, Gatlin D, Lightner D, Hume M, Lawrence A Abstract The present study investigated the effects of short-chain fructooligosaccharides (FOS) as dietary supplements on stress, immune response, gut microbiota, growth, and survivability of three different treatment groups of Pacific white shrimp, Litopaneous vennamei, cultured in a recirculating system. The experiment was conducted over a 35-day trial period. Shrimps were fed diets, 15 times a day using automated feeder, supplemented with GOS at 0%, 0.15%, and 0.30% by weight. Denaturing gradient gel electrophoresis analysis revealed that ...
Source: Journal of Immunoassay and Immunochemistry - October 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of the mucoadhesive strengths of Abelmoschus esculentus and Irvingia gabonensis gums for possible application in veterinary vaccine delivery: the effect of extraction methods.
This study evaluates the effects of different gum extraction methods on the mucoadhesive strengths of Abelmoschus esculentus (AE) and Irvingia gabonensis (IG) gums and the release of vaccine antigen in vaccine-gum formulations. AE and IG gums were extracted employing previously documented methods with acetone or sodium chloride (NaCl) and either oven-dried or freeze-dried. Gum extracts were analyzed for mucoadhesive strengths using a modified rotational cylinder method on animal mucosa. The time taken to detach from the mucosa was taken as the Peak Adhesion Time (PAT). The gum extracts were charged with Peste des petits ru...
Source: Journal of Immunoassay and Immunochemistry - October 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Cyclin D1 and PSA act as good prognostic and clinicopathological indicators for breast cancer.
This study aimed to evaluate immunohistochemical expression of cyclin D1 and PSA in breast carcinoma and their relation to prognosis. It includes 79 specimens of breast invasive duct carcinoma. Overall survival time was available for all patients. There is a statistically significant association between positive PSA expression and lower tumor stage, nodal stage and tumor grade and between negative PSA expression and presence of metastasis (P value = .05, 0.01, 0.03 and 0.011, respectively). There is a highly statistically significant association between low percentage of cyclin D1 expression (
Source: Journal of Immunoassay and Immunochemistry - October 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Dietary supplementation of galactooligosaccharides (GOS) in Pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system and its effects on gut microflora, growth, stress, and immune response.
This study was designed to examine the effects of a prebiotic compound on the immune system, digestive tract histology, and stress physiology of shrimp. The specific effects of dietary supplementation of the prebiotic galactooligosaccharide (GOS or GTGOS) on shrimp health are scarce. This experiment, therefore attempted to evaluate the effects of GOS on growth, survival, intestinal microbiota, stress resistance and immune responses of Pacific white shrimp, Litopaneous vannamei. Over a 35-day trial, shrimps were fed diets, 15 times a day using automated feeder, supplemented with GOS at 0%, 0.25%, and 0.40% by weight. Shrimp...
Source: Journal of Immunoassay and Immunochemistry - October 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunohistochemical detection of naturally occurring porcine Sapelovirus infection in Indian pigs.
Authors: Kumari S, Saikumar G, Desingu PA, Das T, Singh R Abstract We investigated immunohistochemical detection of porcine Sapelovirus (PSV) in naturally infected pigs of different ages. Forty-nine fecal samples, intestinal contents and other tissue samples from dead pigs were screened in previous study using reverse transcription polymerase chain reaction (RT-PCR) for PSV infection. Eight animals were positive for PSV based on RT-PCR examination. Gross lesions were recorded mainly in the large and small intestines. Microscopic examination of intestines showed severe enteritis. Tissue sections of all organs from P...
Source: Journal of Immunoassay and Immunochemistry - October 13, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serum levels of anti-hepatitis B surface antibodies among vaccinated children aged 1 to 12 years in a rural community in Fars Province, southern Iran.
Authors: Arefkhah N, Vafazadeh S, Shahriarirad S, Ghorbani F, Zoghi S, Emami M, Zamiri B, Cheraghi MR, Sarvari J, Sarkari B Abstract The present study aimed to find out the levels of anti-HBsAb among vaccinated children in a rural community in Fars Province, Southern Iran. Blood samples were taken from 550 children, aged 1-12 years (mean 6.4 ± 3.5), in 2017 from three villages in the area. A structured questionnaire was used to get the sociodemographic data of the subjects along with determinants concerning the Hepatitis B. Sera samples were examined for anti-HBsAb, using an ELISA commercial k...
Source: Journal of Immunoassay and Immunochemistry - October 10, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Home pregnancy tests in the hands of the intended user.
The objectives of this study were to investigate the usability and performance of seven visual home pregnancy tests, available in Europe. Part one of the study was home-based and involved volunteers testing a selection of four home pregnancy tests. The tests used and order of use were randomized. Part two, performed at a study site, involved volunteers reading and interpreting the results of the same selection of home pregnancy tests used in part one, but using urine standards representing early pregnancy (25 mIU/mL human chorionic gonadotropin) or a 'not pregnant' (0 mIU/mL human chorionic gonadotropin) sample. The volunt...
Source: Journal of Immunoassay and Immunochemistry - September 27, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The in-house monoclonal antibody against γ-globin chain; Thal N/B, accurately measured F cells in SEA-α thalassemia 1 trait.
The in-house monoclonal antibody against γ-globin chain; Thal N/B, accurately measured F cells in SEA-α thalassemia 1 trait. J Immunoassay Immunochem. 2019 Sep 26;:1-9 Authors: Tangpan L, Jansai T, Kerdpoo S, Kiewkarnkha T, Limweeraprajak E, Tatu T Abstract The monoclonal antibody (mAb) against γ-globin chain clone Thal N/B was produced. It was aimed at measuring the quantity of HbF (α2γ2)-containing red blood cells or F cells by flow cytometry. However, it may cross-react with Hb Bart's (γ4) which is present in the SEA-α thalassemia 1 trait. We measured FC lev...
Source: Journal of Immunoassay and Immunochemistry - September 27, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Comparative analysis of two microbiological tests in the detection of oxytetracycline residue in chicken using ELISA as gold standard.
Authors: Ezenduka EV, Okorie-Kanu OJ, Nwanta JA Abstract The validity of two microbiological methods: Tube (Premi® Test) and Plate (Three Plate Test) Test for the detection of oxytetracycline (OTC) in poultry was done using Enzyme Linked Immunosorbent Assay (ELISA) immunoassay as gold standard. OTC was administered to two groups of birds: intramuscular drug administration (group A) and oral drug administration (group B). Liver and muscle tissue samples from birds in both groups were tested for the presence of OTCwith the Four Plate Test (FPT), Premi® Test and ELISA. For muscle tissues, FPT had a sensit...
Source: Journal of Immunoassay and Immunochemistry - September 24, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Humoral and mucosal immune responses in challenged chickens vaccinated with Infectious bursal disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agents.
In conclusion, use of the mucilage from C. odorata and K. senegalenses in equal proportion has given better enhancement of the response to IBDV vaccination and premise for further investigations for improvement against IBD. PMID: 31544580 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - September 24, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Detection of NRAS G12D and NRAS G13C mutant genes among apparently healthy and haematologic malignant individuals in Federal Capital Territory, Nigeria.
This study was aimed at detecting NRAS G12D and NRAS G13C mutant genes among apparently healthy and haematologic malignant individuals, and to explore their association with some clinical and demographic factors as well as disease status and progression. A total of 200 cfDNAs, 100 each from haematologic malignant patients and blood donors, respectively, were analyzed for the presence of NRAS gene mutations in codons 12 and 13. These mutations were tested using multiplex allele-specific PCR (AS-PCR). The mutations were detected by selective amplification using mutation-specific synthetic oligonucleotides. NRAS G12D and NRAS...
Source: Journal of Immunoassay and Immunochemistry - September 21, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Sandwich antibody-based biosensor system for identification of Mycobacterium tuberculosis complex and nontuberculous mycobacteria.
In this study, we aim to establish an antibody-based biosensor system for the identification of MTBC and NTM infection. Monoclonal antibodies (mAbs) specific for Ag85B proteins of mycobacteria were generated and characterized. The generated anti-Ag85B mAb clones AM85B-5 and AM85B-8 reacted to Ag85B of Mycobacterium spp.; in contrast, clone AM85B-9 specifically reacted to Ag85B of MTBC. By employing the produced mAbs, single and sandwich antibody-based biosensors using bio-layer interferometry were established for determination of Ag85B proteins. The sandwich antibody-based biosensor system was demonstrated to be suitable f...
Source: Journal of Immunoassay and Immunochemistry - August 31, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Efficient short-term expansion of human peripheral blood regulatory T cells for co-culture suppression assay.
Authors: Kanjana K, Paisooksantivatana K, Matangkasombut P, Chevaisrakul P, Lumjiaktase P Abstract Regulatory T cells (Tregs) are a small population of CD4+ lymphocytes and play a key role as suppressors of the immune system, a role that can be identified by employing a co-culture suppression assay. Conventional protocol requires a long period of in vitro expansion of Treg numbers; hence, this study describes an establishment of a co-culture suppression assay using a short-term expansion of peripheral blood (PB) Tregs and autologous T cells (Tconvs) IL-2-pre-cultured in parallel for the same length of time, thereby...
Source: Journal of Immunoassay and Immunochemistry - August 30, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Elevated serum level of IL-4 in neuromyelitis optica and multiple sclerosis patients.
Authors: Tahani S, Dehghani L, Jahanbani-Ardakani H, Shaygannejad V, Fazli A, Hamidavi A, Eskandari N Abstract Mediators have important roles in the pathogenesis of autoimmune diseases. Interleukin 4 (IL-4) is one of the most important cytokines that has a regulatory effect on immune cells. In the current study, the serum level of IL-4 was assessed in the newly diagnosed neuromyelitis optica (NMO) and multiple sclerosis (MS) patients compared to healthy subjects. ELISA technique was used for assessment of the serum level of IL-4, and data analysis was performed by SPSS software. Serum level of IL-4 was elevated in ...
Source: Journal of Immunoassay and Immunochemistry - August 21, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Histochemical and immunohistochemical detection of pathogenic leptospires serovars in tissues of some captive wildlife from a University zoo in Nigeria.
This study has revealed the presence of pathogenic leptospires in some captive wild and zoological garden animals. PMID: 31429374 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - August 21, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Hepatitis C virus seropositivity and the risk factors for transmission among blood donors in some selected centers in Lagos State, Southwest Nigeria.
Conclusion: The relatively high prevalence of HCV infection detected and the risk of transmission among blood donors in this study are of public health importance. Hence, the institution of appropriate measures to stem down the trend of HCV circulation among this population in Nigeria is therefore advocated. PMID: 31378189 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - August 7, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Elevated levels of inflammatory markers in women with rheumatoid arthritis.
Authors: Costa CM, Santos MATD, Pernambuco AP Abstract Rheumatoid arthritis (RA) is an autoimmune and progressive disease. Evidence indicates that inflammatory mediators may contribute to the genesis and/or evolution of this clinical condition. Thus, the objective was to evaluate and compare the plasma levels of Interleukin-17 (IL-17), Tumor Necrosis Factor-Alpha (TNF-α) and Complement 3 (C3) in women with RA and healthy controls (HC), as well as to evaluate the association them with the disease activity. 25 women with RA and 15 HC were recruited. Plasma levels of biomarkers were measured by ELISA. All statis...
Source: Journal of Immunoassay and Immunochemistry - August 3, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunolocalization of MUC1 in chronic plaque psoriasis.
Authors: Abdou AG, Farag AGA, Abdelaziz RA, Samaka RM, Nabil E, Ali M Abstract Psoriasis is a chronic skin inflammatory disease with immunological, hyperproliferative and angiogenic dysfunction. MUC1 is a molecular sensor and signal transductor that responds to external stimuli generating cellular responses, which include cell proliferation, growth, differentiation, migration, invasion, survival and secretion of growth factors, and cytokines. The current study aimed at evaluation of the possible role of MUC1 in the pathogenesis of psoriasis through its immunohistochemical localization in involved and uninvolved pso...
Source: Journal of Immunoassay and Immunochemistry - July 29, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Prevalence of human T-cell lymphotropic virus and the socio-demographic and risk factors associated with the infection among post-natal clinics women in Zaria, Nigeria.
Conclusion: The study established a prevalence an HTLV of 3.2% that HTLV in Zaria therefore routinely screened is necessary. PMID: 31339431 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - July 26, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Comparison of nitric oxide level in Beh çet's disease patients with or without psychiatric comorbidity.
In this study, we investigate whether there was a difference in NO levels among BD patients with accompanying MDD, BD patients with no depressive symptoms and healthy control group. There was a significant difference in NO levels among BD and control group (P
Source: Journal of Immunoassay and Immunochemistry - July 26, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroprevalence of Toxoplasma Gondii antibodies in sheep and goats slaughtered at the Kumasi Abattoir, Ghana.
This study was therefore conducted to estimate the seroprevalence of T. gondii infection sheep and goats sampled from the Kumasi Abattoir in Ashanti Region, Ghana. A total of 347 serum samples collected from 170 sheep and 177 goats were analyzed for the presence of T. gondii antibodies using a commercial ELISA kit. Results of this study estimated the seroprevalence of 23.7% in goats an, 35.9% in sheep. In sheep, 24 (35.82%) out of a total of 67 male samples were positive and 37 (35.92%) out of a total of 104 female samples were positive while in goats, 6 (8.2%) bucks out of a total of 73 were positive while 36 (34.6%) does...
Source: Journal of Immunoassay and Immunochemistry - July 19, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Elevated CSF concentration of CCL3 and CCL4 in relapsing remitting multiple sclerosis patients.
Authors: Soliemani M, Soleymani A, Seyeddirad N Abstract CCL3 and CCL4 are considered as inflammatory cytokines and involved in progression of various neurologic disorders as multiple sclerosis (MS). The aim of this study was to evaluate the association between cerebrospinal fluid (CSF) levels of above mentioned inflammatory cytokines and relapsing remitting multiple sclerosis (RRMS. In this case-control study, 40 unrelated patients (without family relationship) with RRMS and 40 age and sex matched subjects as control group were enrolled. CSF samples obtained from all patients and control group and levels of CCL3 a...
Source: Journal of Immunoassay and Immunochemistry - July 5, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunopharmacology and immunopathology of peptides and proteins in personal products.
Authors: Ahsan H Abstract The beneficial effects promoted from the use of biomolecular substances into the formulations of personal care products are considered useful ingredients in cosmetic and therapeutic applications. Innovations in cosmetics are based on new bioactive formulations such as vitamins, oils, peptides, and protein hydrolysates. In skin care, the monomeric amino acids such as serine, threonine, alanine are common ingredients in cosmetics as they function as natural moisturizing factors which act as water-binding molecules. Amino acids and their salts e.g., arginine, glycine, etc. are also used as ha...
Source: Journal of Immunoassay and Immunochemistry - June 19, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Satureja hortensis induces cell death and inhibited cell cycle progression in K562 myelogenous and Jurkat T cell leukemia cell lines.
Authors: Asadipour M, Amirghofran Z Abstract Several plants of Satureja genus have shown anti-tumor activity. We investigated the antileukemia effects of different fractions of Satureja hortensis (Summer savory). The growth inhibitory effect of S. hortensis fractions on K562 and Jurkat leukemia cells were determined by MTT assay. The most effective fractions were analyzed by flow cytometry and colorimetric assay for apoptosis induction and cell cycle changes. Various fractions from S. hortensis showed growth inhibitory effects on leukemia cells, among them two hexane and dichloromethane fractions with IC50 values o...
Source: Journal of Immunoassay and Immunochemistry - June 19, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The seroprevalence of Sarcoptes scabiei var. canis and its associated risk factors in dogs in Ibadan, Southwest Nigeria.
This study investigated the use of SARCOPTES-ELISA DOG KIT as a diagnostic tool in detecting anti-mite antibodies, establishing the prevalence of scabies in dogs and the associated risk factors of canine mange occurrence. A total of 384 dogs (149 males and 235 females) were randomly sampled from eight local government areas. The prevalence of canine sarcopticosis was found to be 67.45% (259/384) using the ELISA kit. ELISA test had a 96.4% sensitivity against microscopy test (21.42%) as skin scrapping with microscopy demonstrated mites in 12 out of 56 dogs, while ELISA detected antibodies in 54 out of the 56 dogs presented ...
Source: Journal of Immunoassay and Immunochemistry - June 19, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Effect of dietary status on plasma-fractionated metanephrines in healthy individuals measured by Elisa.
This study was conducted at the Chemical Pathology Laboratory, Royal Hospital, Oman. Three plasma samples were collected from each participant (16 male and 16 female) over three consecutive days (day one: dietary restriction,day two: excess intake of restricted foods, day three: random sample following the typical diet for each participant). Samples were collected and centrifuged to obtain the plasma, which was then stored at -20°C prior to analysis. Metanephrine and normetanephrine concentrations were measured by comparative ELISA. Plasma metanephrine and normetanephrine measured under the three different dietary cond...
Source: Journal of Immunoassay and Immunochemistry - June 19, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Effects of resveratrol on shedding and pathological dynamics in experimental B. melitensis infection in dexamethasone-treated nonpregnant Boer goats.
This study evaluated relationship between Brucella melitensis infection, shedding dynamics, dexamethasone-induced stress, pathological alterations and resveratrol ameliorative effects in goats. Twelve nonpregnant goats were divided into four groups A, B, C, and D of three animals each. Groups A and B were administered 107 CFU/mL of B. melitensis ocularly, 21 days prior to 7 days consecutive administration of dexamethasone (2 mg/kg). Group A was further administered resveratrol (5 mg/kg) intravenously for 5 consecutive days from day 31 post B. melitensis inoculation. Group C was administered similar dose of B...
Source: Journal of Immunoassay and Immunochemistry - June 5, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Singlet oxygen species and systemic lupus erythematosus: a brief review.
Authors: Ahmad R, Ahsan H Abstract Reactive oxygen species (ROS) are implicated in inflammatory, autoimmune, and neurodegenerative diseases. The activation, proliferation, and apoptosis of immune cells are dependent on the controlled production and elimination of ROS. However, under chronic inflammatory conditions, large amounts of ROS generated are a major cause of many human diseases. The electronically excited molecular oxygen species known as singlet oxygen (1O2) is a form of ROS and is one of the major cytotoxic species in eukaryotic cells. ROS are known to cause DNA damage leading to strand breaks, base damag...
Source: Journal of Immunoassay and Immunochemistry - May 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The investigation of relevancy between PIAS1 and PIAS2 gene expression and disease severity of multiple sclerosis.
This study aims to evaluate the gene expression of these factors in multiple sclerosis (MS) patients compared to healthy individuals and correlate them with the severity of MS. MATERIALS AND METHODS: Sixty participants, including 30 patients with MS and 30 healthy controls were studied. The expression of PIAS1 and PIAS2 genes in peripheral blood samples of all participants was measured by real-time PCR. The severity of MS was evaluated using the Expanded Disability Status Scale (EDSS). Finally, we evaluated the correlation between the expression of PIAS1 and PIAS2 genes with disease severity. RESULTS: The expressio...
Source: Journal of Immunoassay and Immunochemistry - May 16, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research