Serological detection of human parvovirus B19 infection and associated risk factors among pregnant women in Jigawa State, Nigeria.
This study aims to determine the seroprevalence, sociodemographic, and risk factors of human B19V infection among present women attending antenatal clinics of Jahun General Hospital, Nigeria. Between 2 February and 30 June 2019, blood samples were collected from 200 consented pregnant women and analyzed for anti-B19V IgM and IgG using commercially available enzyme-linked immunosorbent assay (ELISA). Sociodemographic and risk factors of subjects were collated through pre-tested structured questionnaires. Data generated were statistically analyzed for the association of anti-B19V and subjec...
Source: Journal of Immunoassay and Immunochemistry - February 18, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis.
This study investigated dexamethasone-treatment, shedding routes, tissue antigen distribution, and pathology of caprine Brucellosis. Eighteen non-pregnant goats were randomly grouped into A, B, and C. Group A was administered dexamethasone for 7 days at 2 mg/kg before inoculating 0.5 mL B. melitensis at 107 CFU ocularly while group B was inoculated 0.5 mL B. melitensis only, and C as control negative. Blood samples, ocular, nasal, and vaginal swabs were obtained for evaluation. Three goats were sacrificed from each group at days 21 and 42 post-inoculation (pi) and selected tissues collected for PCR, histopathology, an...
Source: Journal of Immunoassay and Immunochemistry - February 13, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of oral phytogenic microbeaded Newcastle Disease vaccine delivery in indigenous chicken.
This study describes the evaluation of microbeaded oral vaccine delivery for Newcastle Disease (ND) in village chicken. Microbeads containing vaccine were prepared by ionotropic-gelation technique using aluminum sulfate. Lasota Vaccine strain (2 g) was well mixed with Boswellia caterii gum extract at ratio 1:1. The wet beads were washed twice with distilled water and dried at 37℃ overnight. Microbeads without vaccine were prepared as control. A tablet dissolution machine was used to evaluate peak adhesion time (PAT). Sixty local chickens sourced from a recognized breeder were separated into four groups for in in-viv...
Source: Journal of Immunoassay and Immunochemistry - February 10, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Targets and assay types for COVID-19 diagnosis.
Authors: Bostan M, Ataman M, Bostan IS, Bleotu C Abstract The lack of complete information on the immune response dynamics to infection with severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) has led to the use of mainly molecular tests such as reverse transcription PCR (RT-PCR) to diagnose Coronavirus 2019 disease (COVID-19). Although remarkable progress has been made in developing effective RT-PCR kits, the lack of specific equipment required to perform this technique in all clinical laboratories limits its widespread use. In the case of COVID-19, these tests can be used for the triage of symptomatic pa...
Source: Journal of Immunoassay and Immunochemistry - February 6, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Comparative expression profile of CD10 and cyclin D1 in cutaneous histiocytofibroma and dermatofibrosarcoma.
In this study, we aimed to compare the expression of CD10 and cyclin D1 in 15 cases of DFSP and 15 cases of HF and discuss their potential contribution in the differential diagnosis. PMID: 33444077 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - January 16, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Cyclin D1: potential utility as marker for Langerhans cell histiocytosis.
Authors: Ben Rejeb S, Charfi L, Sahraoui G, Boujelben N, Mrad K, Doghri R Abstract Langerhans cell histiocytosis (LCH) is a rare disorder of unknown etiopathogenesis. Diagnosis is based on the identification of CD1a positive histiocytic infiltrate. Activation of the mitogen-activated-protein-kinase (MAPK) is constantly observed in LCH and therefore downstream markers such as cyclin D1 may be a useful marker for LCH. The aim of this study was to investigate the expression of cyclin D1 in LCH. We assessed the immunohistochemical expression of cyclin D1 (clone SP4-R) in series of 16 cases of confirmed LCH. Expression ...
Source: Journal of Immunoassay and Immunochemistry - January 16, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Increased metformin dosage suppresses pro-inflammatory cytokine levels in systemic circulation and might contribute to its beneficial effects.
Authors: Amoani B, Sakyi SA, Mantey R, Laing EF, Ephraim RD, Sarfo-Katanka O, Koffie S, Obese E, Afranie BO Abstract Type 2 Diabetes Mellitus (T2DM) is a metabolic disorder, characterized by persistent elevation of blood glucose either due to insulin resistance or insulin insufficiency. Metformin is the recommended first choice of drug for the management of T2DM and is known to improve insulin sensitivity and prevents hyperglycemia by reducing chronic inflammation. T-helper type 1 (Th1) and type 17 (Th17) cells, are important pro-inflammatory CD4+ T cell subsets secreting TNF-α, and INF-γ (Th1), and int...
Source: Journal of Immunoassay and Immunochemistry - January 16, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Dissemination kinetics and pathology of canine Leptospira icterohaemorrhagiae isolate in a guinea pig infection model.
This study was designed to establish leptospire dissemination kinetics and patho-morphological changes associated with these orangs in the guinea pig infection model using cultural isolation (CI), polymerase chain reaction (PCR), Warthin Starry silver stain (WSss), immunohistochemistry (IH), and transmission electron microscopy (TEM). Twenty guinea pigs were inoculated intra-peritoneally with a low dosage of 1 × 107 Leptospira interrogans serovar Icterohaemorrhagiae and 10 as control using distilled water. The guinea pigs were sacrificed at post-infection day (p.i.d.) ½, 1, 3, 5, and 7 followed by th...
Source: Journal of Immunoassay and Immunochemistry - January 16, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serosurvey of parvovirus B19 and cytomegalovirus infections among female university students in Shiraz, Southern Iran.
This study aimed to investigate the frequency of IgM and IgG antibodies against parvovirus B19 and CMV in female university students in Shiraz, in Fars province, Southern Iran. In this cross-sectional study, 370 female university students were included. Blood samples were collected from each participant and tested for anti-parvovirus B19 and CMV IgG and IgM antibodies, using commercial ELISA kits. The mean age of the participants was 24 (±7)years. Out of 370 participants, 327 (88.4%) and 9 (2.4%) were positive for IgG and IgM antibodies against CMV. Moreover, 211 (57.0%) and 4 (1.1%) of the participants were respect...
Source: Journal of Immunoassay and Immunochemistry - January 13, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Considerations on clinical biomarkers analyses using immunoassays.
Authors: Moscovitch-Lopatin M PMID: 33428524 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - January 13, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Effects of cannabidiol (CBD) on the inhibition of melanoma cells in vitro.
Authors: Burch R, Mortuza A, Blumenthal E, Mustafa A Abstract Cannabidiol (CBD) is one of the most popular emerging plant extracts that is being investigated for its wide range of potential health benefits. This experiment tests how B16 mice melanoma cells, are affected by four different concentrations (0.2 mg/mL, 0.04 mg/mL, 0.008 mg/mL and 0.0016 mg/mL) of 99% CBD oil. The results of this experiment demonstrate that CBD significantly inhibited melanoma cell growth in-vitro at 0.2 mg/mL and 0.04 mg/mL. This shows that CBD has the potential to inhibit melanoma cell growth in vertebrates, namely mice. PMID: 3342...
Source: Journal of Immunoassay and Immunochemistry - January 13, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunotherapies and COVID-19 related Neurological manifestations: A Comprehensive Review Article.
Authors: Anwar MM Abstract In December 2019, an outbreak of pandemic severe respiratory distress syndrome coronavirus disease 2019 (COVID-19) initially occurred in China, has spread the world resulted in serious threats to human public health. Uncommon neurological manifestations with pathophysiological symptoms were observed in infected patients including headache, seizures, and neuroimmunological disorders. Regardless of whether these neurological symptoms are direct or indirect casual infection relationship, this novel viral infection has a relevant impact on the neuroimmune system that requires a neurologist's ...
Source: Journal of Immunoassay and Immunochemistry - January 6, 2021 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Histological findings in skeletal muscle of SARS-CoV2 infected patient.
Authors: Mageriu V, Zurac S, Bastian A, Staniceanu F, Manole E Abstract Autopsies represent medical procedures through which the causes of patients' deaths are determined or, through tissue sampling and microscopic examination of slides in usual stains or special tests, one can offer the basis for understanding the physiopathological mechanisms that contribute to the patients' death Histological findings of tissue samples from patients who have died of COVID-19 have been mainly orientated to lung, heart, liver, kidney damage with a small percent of them following other organs, but none has, to our knowledge, studie...
Source: Journal of Immunoassay and Immunochemistry - December 30, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Herbal medicine, a reliable support in COVID therapy.
Authors: Grigore A, Cord D, Tanase C, Albulescu R Abstract At present, specific therapies for COVID-19 are not well established, being certain only that the immune system plays a decisive role in the initiation and progression of the disease. Plants have given and continue to give compounds with great efficiency and low toxicity, some of them being a starting point for extremely effective synthetic substances. Although herbal remedies are used mainly for preventive purposes, there are also guidelines issued by some countries that indicate the use of traditional remedies for different stages of COVID-19 disease.Euro...
Source: Journal of Immunoassay and Immunochemistry - December 30, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Comparative analysis of complement-dependent lymphocytotoxicity crossmatch and flow cytometry crossmatch results versus Luminex single-antigen bead-based donor-specific IgG class I antibody MFI values in live related renal transplant cases; a retrospective observation in 102 cases.
Authors: Pandey P, Pande A, Kumar Devra A, Kumar Sinha V, Prasad Bhatt A Abstract The aim of this study was to compare the results of solid phase assay and cell-based assay, and explore the near-accurate DSA-MFI-cutoff value detected on solid phase assay above which the cell-based assay would show a positive result. In this retrospective study, 102 prospective renal transplant recipients were tested for the presence of donor-specific antibodies (DSAs) by cell-based assay (T-cell-CDC-AHG-XM and T-cell-IgG-FC-XM) and solid phase assay (class-I-IgG-L-SAB) with their corresponding donor. Among the 40 patients in the gr...
Source: Journal of Immunoassay and Immunochemistry - December 30, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Understanding COVID-19 immunity: reality and challenges.
Authors: Tanase C, Albulescu R, Neagu M PMID: 33350881 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - December 24, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The diagnostic value of golgi protien-73 and DICKKOPF-1 in hepatocellular carcinoma.
This study included 60 patients: 30 patients with HCC, 30 patients with cirrhosis together with 30 healthy controls. There was a highly significant difference in GP73 and DKK1 between all the studied groups. There was a highly significant positive correlation between GP73 and DKK1 and a significant negative correlation between (GP73 and albumin) and (DKK1 and ALT) in group I. GP73, DKK-1 correlated with the number, overall size, and lymph node metastasis. GP73 and DKK1 can serve as diagnostic serologic markers for early HCC as they had high sensitivity and specificity, correlated with the aggressiveness of the tumor superi...
Source: Journal of Immunoassay and Immunochemistry - December 8, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Human health and snails.
Authors: Dhiman V, Pant D Abstract Snails can provide a considerable variety of bioactive compounds for cosmetic and pharmaceutical industries, useful for the development of new formulations with less toxicity and post effects compared to regular compounds used for the purpose. Compounds from crude extract, mucus, slime consist of glycans, polypeptides, proteins, etc., and can be used for curing diseases like viral lesions, warts, and different dermal problems. Some particular uses of snails involve treating post-traumatic stress. Micro RNA of Lymnaea stagnalis, was known to be responsible for the development of lo...
Source: Journal of Immunoassay and Immunochemistry - November 25, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Correlation between immunohistochemical expression of Ki-67and P63 and aggressiveness of urinary bladder urothelial carcinoma.
Authors: Abdallah MM, Wahbbah MA, Selem M, Abdou AG, Sultan SM Abstract Urothelial carcinoma is the most common urinary malignancy with a wide proportion of cancer morbidity and mortality. The aim of the present study is to evaluate Ki-67 and p63 immunoexpression and their correlation with grade and stage of bladder urothelial carcinoma. Fifty cases of bladder urothelial carcinoma were investigated and were submitted to immunohistochemical staining for p63 and Ki-67, which were assessed qualitatively and quantitatively. A high percentage of p63 immunoexpression showed a significant association with low-grade tumors (P
Source: Journal of Immunoassay and Immunochemistry - November 21, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Stratification of urinary bladder carcinoma based on immunohistochemical expression of CK5, CK14 and CK20.
Authors: Al-Sharaky DR, Abdelwahed M, Asaad N, Foda A, Abdou AG Abstract Molecular subtyping of urothelial carcinoma (UC) is similar to that of breast cancer and is based on the developmental biology approach. The aim of the present study is to assess the prognostic impact of CK5, CK14, and CK20 expression in urinary bladder cancer (UBC) with the potential to stratify them into different subtypes. The current study examined the immunohistochemical expression of CK5, CK14, and CK20 in 90 specimens of UBC. CK5 was expressed in 81.1% of the cases and was significantly associated with old age, muscle invasion, presence...
Source: Journal of Immunoassay and Immunochemistry - November 21, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serum amyloid A in healthy subjects: assessment of reference value using ELISA method.
Authors: Carbone T, Pafundi V, Schievano C, Assunta D, Padula MC, Giordano M, Canora G, Lazzari C, Padula AA, D'Angelo S Abstract Serum amyloid A (SAA) is a family of acute-phase reactants. The rise of SAA concentration in blood circulation during the acute-phase response is a clinical marker of active inflammation. Despite its practical and analytical advantages, SAA measurement by enzyme-linked immunosorbent assay (ELISA) has been used mainly as a research tool rather than for the routine laboratory testing. This may be partly explained by the lack of robust reference data in the literature for the different comm...
Source: Journal of Immunoassay and Immunochemistry - October 31, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

ELISA versus LUMINEX assay for measuring mouse metabolic hormones and cytokines: sharing the lessons I have learned.
Authors: Mountjoy KG Abstract Enzyme-linked immunosorbent assay (ELISA) has long been the standard for quantitative analysis of metabolic hormones and cytokines. LUMINEX multiplex bead array assays were developed as cost- and time-effective alternatives to ELISA, but they are the only cost- and time-effective if they provide informative data. Here, I show that using half-volume of reagents for an adiponectin single-plex LUMINEX assay and a 6-plex LUMINEX xMAP mouse metabolic bead assay, produces reliable data and increases assay cost-effectiveness. I provide direct comparisons between LUMINEX assay and ELISA for qu...
Source: Journal of Immunoassay and Immunochemistry - October 29, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Hepatitis B virus infection among Nigerian pediatric population.
Authors: Adetunji SO, Ajite A, Adetunji BI, Olaniran OB, Donbraye E Abstract Hepatitis B infection has continued to be a growing global health concern in spite of the discovery and administration of hepatitis B vaccines over the years. The focus of this study was to evaluate the burden of this infection among asymptomatic pediatric population. Children, who visited the hospital between March and August 2018, were recruited into this study. Blood samples were collected after obtaining informed consent. The samples were processed and screened for HBsAg. Data were analyzed using chi-square statistic. Six (2.9%) of the...
Source: Journal of Immunoassay and Immunochemistry - October 28, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Patho-morphological and immunohistochemical diagnosis of fibrous tissue pulmonary hamartoma in young buffaloes.
Authors: Kamdi B, Singh R, Singh V, Singh S, Kashyap G Abstract Hamartoma is a developmental disorder characterized by the presence of well-differentiated tissue, of multiple types, in excess at its normal location. The present study describes a fibrous tissue pulmonary hamartoma in two buffaloes. Grossly, there were large pale white masses present on the left diaphragmatic lobe in both cases. Microscopic examination of the lungs revealed that the bronchioles were dilated and showed many terminal respiratory bronchioles lined by a single layer of cuboidal epithelium supported by thick bands of collagenous tissue. A...
Source: Journal of Immunoassay and Immunochemistry - October 25, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

IInteraction of organophosphate pesticide chlorpyrifos with alpha-2-macroglobulin: Biophysical and molecular docking approach.
Authors: Dixit S, Zia MK, Siddiqui T, Ahsan H, Khan FH Abstract Organophosphate class of pesticides causes neurotoxicity and carcinogenicity in humans. Once inside the human body, these pesticides often interact with plasma proteins, such as alpha-2-macroglobulin (α2M) which is the key anti-proteinase. Our work focuses on the structural and functional alteration of α2M by chlorpyrifos (CPF), a member of organophosphates. We explored the binding interaction between alpha-2-macroglobulin and CPF by using UV absorption and fluorescence spectroscopy (steady state and synchronous), circular dichroism and mol...
Source: Journal of Immunoassay and Immunochemistry - October 24, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The bumpy road to achieve herd immunity in COVID-19.
Authors: Neagu M Abstract Herd immunity is a form of indirect protection that is offered to the community when a large proportion of individuals contained in the community are immune to a certain infection. This immunity can be due to vaccination or to the recovery post-disease. Effective herd immunity in SARS-CoV-2 infection has several hurdles upon achievement. Herd immunity cannot be obtained concomitantly in many geographical areas because the areas have different population density and the societal measures to contain the spreading are different. A proportion of 50-66% of the population needs to be immunized n...
Source: Journal of Immunoassay and Immunochemistry - October 24, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Neuroprotective effects of probiotics bacteria on animal model of Parkinson's disease induced by 6-hydroxydopamine: A behavioral, biochemical, and histological study.
Authors: Alipour Nosrani E, Tamtaji OR, Alibolandi Z, Sarkar P, Ghazanfari M, Azami Tameh A, Taghizadeh M, Banikazemi Z, Hadavi R, Naderi Taheri M Abstract Parkinson's disease (PD) is an age-associated, progressive, and common neurodegenerative disorder. It is characterized by dopaminergic neuron degeneration in the substantia nigra pars compacta. The involvement of oxidative stress, inflammation, and dysbiosis in PD has been confirmed and probiotics also have the ability to regulate the mentioned mechanisms. Here, we assessed probiotics supplementation effects on experimental model of PD. Thirty Male Wistar rats w...
Source: Journal of Immunoassay and Immunochemistry - October 22, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of three commercially available ELISA kits for the determination of chromogranin A.
Authors: Eeckhout K, Van Cotthem K, Guerti K Abstract Chromogranin A (CgA) is currently the most valuable tumor biomarker for diagnostic work-up, management and follow-up of neuro-endocrine tumors (NET). The aim of our study was to compare three different commercially available CgA ELISA kits and to evaluate their analytical and clinical performance. CgA was measured with three different commercial ELISA kits on leftover sera from 40 patients: Chromoa R assay (Cis), Hu chromogranin A ELISA (Dia), and Neolisa chromogranin A (Euro). Analytical and clinical performance was evaluated by measuring precision, area under ...
Source: Journal of Immunoassay and Immunochemistry - October 13, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

High seroprevalence of viral hepatitis among animal handlers in Abeokuta, Ogun State, Nigeria.
This study aimed at determining the prevalence of Hepatitis B, C, and E viruses among animal handlers in Abeokuta, southwestern Nigerian. A total of 156 subjects were recruited for the study. Sociodemographic and risks factors data were fetched from subjects using interviewer-administered questionnaire. Blood samples were collected via venepuncture and tested for HCV, HBV, and HEV using ELISA technique. Results were analyzed using SPSS software version 21.0 and P value ≤ 0.05 was considered significant. The prevalence of HCV, HBV, and HEV were 46 (29.5%), 20 (12.8%), and 4 (2.6%) respectively while 6 (3.8%), 1 (0.6%), a...
Source: Journal of Immunoassay and Immunochemistry - October 13, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The effect of temozolomide in combination with doxorubicin in glioblastoma cells in vitro.
CONCLUSION: While the results may be discouraging, they present an interesting prospect into how certain drug interactions can impact treatment response. PMID: 33021886 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - October 8, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serological evidence of HIV, Hepatitis B, C, and E viruses among liver disease patients attending tertiary hospitals in Osun State, Nigeria.
Authors: Oluremi AS, Opaleye OO, Ogbolu DO, Alli OAT, Ashiru FT, Alaka OO, Suleiman IE, Enitan SS, Adelakun AA, Adediji IO, Olowoyeye EA, Adewumi OO, Ayodele TO, Ogunleke OA Abstract Hepatitis infection in HIV positive individuals with liver diseases causes high mortality worldwide. HIV worsens the pathological effect of hepatitis viruses and potentiates reactivation of latent hepatitis infections due to reduced immunity. This research therefore aimed to study the occurrence of HIV and hepatitis viruses among liver diseases patients (LVDP) attending tertiary hospitals in Osun State, southwestern Nigeria. A total of...
Source: Journal of Immunoassay and Immunochemistry - September 26, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Intranasal Peste des petits ruminants virus vaccination of goats using Irvingia gabonensis gum as delivery system: hematological and humoral immune responses.
Authors: Ezeasor C, Shoyinka S, Emikpe B, Bodjo C PMID: 32970525 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - September 26, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Competitive immunochromatographic test strips for the rapid semi-quantitative analysis of the biologically active bitter glycoside, amarogentin.
Authors: Nuntawong P, Lohseethong K, Juengwatanatrakul T, Yusakul G, Putalun W, Tanaka H, Sakamoto S, Morimoto S Abstract Amarogentin (AG), a biologically active secoiridoid glycoside, is responsible for the efficacy of Gentianaceae based medications. Thus, qualitative and quantitative analyses of AG are of significance for batch to batch quality control purposes. By conjugating colloidal gold nanoparticles with the AG-specific monoclonal antibody, MAb 1E9, we were able to develop a single-step competitive immunochromatographic assay (ICA) for simple quantification of the AG content in plant samples. With a limit o...
Source: Journal of Immunoassay and Immunochemistry - September 9, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Design and evaluation of scFv-RTX-A as a novel immunotoxin for breast cancer treatment: an in silico approach.
Authors: Samavarchi Tehrani S, Gharibi S, Movahedpour A, Goodarzi G, Jamali Z, Khatami SH, Maniati M, Ranjbar M, Shabaninejad Z, Savardashtaki A, Taheri-Anganeh M Abstract Human epidermal growth factor receptor 2 (HER2) is overexpressed in breast cancer (BC) patients. Hence, immunotherapy is a proper treatment option for HER2-positive BC patients. Accumulating evidence has indicated that immunotoxin therapy is a novel approach to improve the potency of targeted therapy. Immunotoxins are antibodies or antibody fragments coupled with a toxin. We designed an immunotoxin. The physicochemical properties were evaluated u...
Source: Journal of Immunoassay and Immunochemistry - August 29, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

High Viral Hepatitis Infection among Pregnant Women Attending Antenatal Clinic in Adeoyo Maternity Teaching Hospital Ibadan (AMTHI) Oyo State, Nigeria.
This study aimed to determine the viral hepatitis in pregnant women attending antenatal clinic, AMTHI. Informed consent questionnaire was administered before blood collection via venipuncture. a total of 904 pregnant women plasma samples were tested for HBV, HCV, and HEV using ELISA kit. Data was analyzed using packages within SPSS software and P ≤ 0.05 was considered significant. Out of 904 samples analyzed, the overall prevalence of hepatitis infections among pregnant women attending antenatal clinic in AMTHI was 66(7.3%). High prevalence of the hepatitis infections was found among young women within the age grou...
Source: Journal of Immunoassay and Immunochemistry - August 26, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

β-arrestin 1 transfection induced cell death in high grade glioma in vitro.
In conclusion, we showed that β-arr 1 overexpression inhibits cell proliferation in the 18 cell line but only has a very modest effect on treatment response with the alkylating agent TMZ. PMID: 32807003 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - August 20, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Peripheral blood hematology, plasma biochemistry, and the optimization of an in vitro immune-based assay in the brown watersnake (Nerodia taxispilota).
Authors: Haskins DL, Brown MK, Meichner K, Tuberville TD, Gogal RM Abstract Reptiles represent a phylogenetic lineage that provides a unique link between ectothermic anamniotes and endothermic amniotes. Compared to mammalian and avian species, our understanding of the reptilian immune system is greatly lacking. This gap in knowledge is largely due to an absence of established immune-based assays or specific reagents for these species. In the present study, brown watersnakes (Nerodia taxispilota) were live-captured in the wild, sexed, weighed, measured, bled via the caudal vein, and released. At 24 hr post-coll...
Source: Journal of Immunoassay and Immunochemistry - August 20, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The effect of smoking on latent tuberculosis infection susceptibility in high risk individuals in Iran.
This study is the first to analyze the relationship between smoking in patients with respiratory diseases and LTBI susceptibility in Iran by IGRAs, which proposes cigarette smoking as a powerful risk factor for LTBI. PMID: 32799615 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - August 19, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

An enzyme immunoassay for determining albumin in human urine samples using an ultra-microanalytical system.
Authors: Del Valle R, Cazanave Mora JM, Carrazana San Martín NL, Hernández Pérez L, Legrá Torres ME, López Cisneros R, Ordaz Ramos RP, Rodríguez AM, Valdivia Álvarez I Abstract Microalbuminuria is a term to describe a moderate increase in the level of albumin in urine. It is an important prognostic marker for kidney damage in diseases such as diabetes mellitus and hypertension. A simple sandwich-type ultramicroELISA assay (UMELISA) has been developed for the measurement of albumin in human urine samples. Strips coated with a high affinity monoclonal antibody directed...
Source: Journal of Immunoassay and Immunochemistry - August 19, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serodiagnosis of human cystic echinococcosis based on recombinant antigens B8/1 and B8/2 of Echinococcus granulosus.
CONCLUSION: Simultaneous usage of AgB8/1 and AgB8/2 increased the test sensitivity for the diagnosis of CE. Furthermore, the specificity of AgB8/1 and AgB8/2 combination was more than AgB8/1 and equal to AgB8/2 alone. The findings revealed that the simultaneous usage of AgB8/1 and AgB8/2 could be a suitable approach for the diagnosis of CE. PMID: 32795213 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - August 17, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A possible risk of environmental exposure to HEV in Ibadan, Oyo State, Nigeria.
Authors: Olayinka A, Ifeorah IM, Omotosho O, Faleye TOC, Odukaye O, Bolaji O, Ibitoye I, Ope-Ewe O, Adewumi MO, Adeniji JA Abstract Hepatitis E virus (HEV) infection is both a major public health concern and emerging global health concern, with a documented incidence of 20 million, 3.4 million clinical cases, 70,000 deaths, and 3,000 stillbirths. The aetiologic agent, HEV is a primarily enterally transmitted hepatotropic virus. Fecal samples were collected from three selected pig farms across Ibadan, South-west Nigeria. Randomly picked samples were pooled per unit pen and fecal suspensions prepared were subjected t...
Source: Journal of Immunoassay and Immunochemistry - August 14, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunohistochemical expression of BCAP 31 in chronic plaque psoriasis.
Authors: Abdou AG, Diab A, Marae A Abstract Psoriasis is a common chronic skin inflammatory disease characterized by an exaggerated proliferation of keratinocytes. B-cell receptor-associated protein 31 (BCAP 31) plays critical roles in induction of proliferation and apoptosis. The current study aimed at evaluation of the immunohistochemical localization of BCAP 31 in psoriatic skin compared to normal skin in addition of correlating BCAP31 expression with the clinical and pathological parameters of psoriasis. The present study was carried out on skin biopsies from 30 psoriatic patients and 10 normal skin (control gr...
Source: Journal of Immunoassay and Immunochemistry - July 3, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of two (02) platforms for chemiluminescence-based detection of anti-rubella IgG antibodies in a sub-Saharan country, C ôte d'Ivoire.
This study aimed to evaluate two chemiluminescence platforms, Architect i2000SR and Maglumi 800 for rubella biological diagnosis in Côte d'Ivoire. Blood samples were taken from 113 pregnant women aged 15 to 30 in prenatal care. Samples were analyzed for Rubella IgG detection at the NBTS laboratory on the evaluated platforms and the Cobas e601 used as a reference. The majority of women were in their second trimester of pregnancy. Among them, only 13.3% were vaccinated against rubella. The evaluated platforms showed good precision with coefficients of variation>10%. Regarding analytical performances, sensitivities w...
Source: Journal of Immunoassay and Immunochemistry - July 1, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Classifying multiple lung cancers using morphological features: a meta-analysis.
Authors: Mlika M, Zorgati M, Mezni FE Abstract Multiple lung cancers may be intrapulmonary metastases or multiple primaries. Management and prognosis of both entities are different and make the pathologist's role challenging. In fact, distinguishing both entities may be difficult especially when the tumors present the same microscopic subtype. The microscopic diagnoses of these tumors have been improved based on the 2015 WHO classification. The aim of the authors was to assess the diagnostic value of morphologic features in comparison to the gold standard test represented by molecular testing in the distinction bet...
Source: Journal of Immunoassay and Immunochemistry - June 26, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Bilirubin binding affects the structure and function of alpha-2-macroglobulin.
Authors: Ali SS, Zia MK, Siddiqui T, Ahsan H, Khan FH Abstract Bilirubin is an endogenous antioxidant that is a metabolite of the heme in red blood cells (RBC). In blood, bilirubin is associated with albumin to form a water-soluble complex, known as unconjugated bilirubin. Alpha-2-macroglobulin (α2M) is a proteinase inhibitor found in the plasma of vertebrates. In the present study, we have investigated the interaction of photo-illuminated bilirubin with serum α2M using various biophysical and thermodynamic techniques. The binding of bilirubin to α2M leads to various functional and structural chan...
Source: Journal of Immunoassay and Immunochemistry - June 26, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Diagnostic performance of Echinococcus granulosus protoscolices antigens in the serodiagnosis of human cystic echinococcosis.
This study aimed to evaluate the efficacy of ELISA in the diagnosis of human cystic echinococcosis (CE), using parasite protoscolices antigens. Liver hydatid cysts were isolated from sheep infected with hydatid cysts and the protoscolices were isolated from the hydatid cyst fluid. Protoscolices crude antigen was prepared by mechanical disruption, plus freeze-thawing and sonication methods. Thirty sera samples of confirmed hydatid cyst patients, 30 samples of healthy individuals, and 30 samples of people with other infections were collected and the samples were evaluated in an ELISA system, using the crude protoscolices ant...
Source: Journal of Immunoassay and Immunochemistry - June 21, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Dominant IgM synthesis against the soluble form of the prevailing variant surface glycoprotein from TeAp-N/D1 Trypanosoma equiperdum throughout the experimental acute infections of horses with non-tsetse transmitted Trypanozoon parasites.
Authors: Uzcanga GL, Bubis J Abstract Two horses were infected with distinct non-tsetse transmitted Trypanozoon Venezuelan stocks, namely TeAp-N/D1 Trypanosoma equiperdum and TeAp-El Frio01 Trypanosoma evansi. Preceding reports have revealed that a 64-kDa antigenic glycopolypeptide (p64), which is the soluble form of the predominant variant surface glycoprotein from TeAp-N/D1 T. equiperdum, can be used as a good antigen for immunodiagnosis of animal trypanosomosis. Here, the course of the experimental acute infection in both horses was monitored by evaluating total anti-p64 IgG and particular anti-p6...
Source: Journal of Immunoassay and Immunochemistry - June 13, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Antibodies to oxidized lipoproteins in human normal plasma, detection and analysis.
Authors: Gasparyan VK, Mikaelyan MV, Poghosyan GG Abstract In the article, presence of antibodies to protein components of oxidized human donor plasma was demonstrated. It was shown that normal plasma oxidized by CuCl2, FeSO4, or potassium ferricyanide acquired ability to bind with human IgG-sensitized polystyrene latex particles and colloidal gold nanoparticles. Ability of these antibodies to bind to oxidized components strongly depends on time of oxidation. Moreover, Sepharose 2B conjugated with human IgG has affinity to component of such plasma. Molecular weight analysis demonstrates that this component eluted a...
Source: Journal of Immunoassay and Immunochemistry - June 7, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Mast cell activation markers for in vitro study.
Authors: Sahid MNA, Kiyoi T Abstract Mast cells (MCs) are well known for their role in allergic conditions. This cell can be activated by various types of secretagogues, ranging from a small chemical to a huge protein. Mast cell activation by secretagogues triggers the increase in intracellular calcium (iCa2+) concentration, granule trafficking, and exocytosis. Activated mast cells release their intra-granular pre-stored mediator or the newly synthesized mediator in the exocytosis process, in the form of degranulation or secretion. There are at least three types of exocytosis in mast cells, which are suggested to c...
Source: Journal of Immunoassay and Immunochemistry - June 7, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Inflammation, diet, and type 2 diabetes: a mini-review.
Authors: Jafaripour S, Sedighi S, Jokar MH, Aghaei M, Moradzadeh M Abstract Inflammation is a common feature of type 2 diabetes (T2D). Inflammatory cytokines increase in patients with type 2 diabetes, metabolic syndrome, and heart disease. Various types of cells can produce inflammatory cytokines and then release them into the bloodstream, where their complex interactions with target tissues raise a tissue-specific immune response. This review focused on C-reactive protein (CRP), tumor necrosis factor (TNF)-α as an inflammatory cytokine, and adiponectin produced by adipose tissues. Despite the major role of c...
Source: Journal of Immunoassay and Immunochemistry - May 15, 2020 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research