First Report of Immunohistochemical Detection of Peste Des Petit Ruminants, Parainfluenza 3 and Respiratory Syncytial Viral Antigens in Lungs of Nigerian Goats.
This study determined the of involvement of PPR, PI3 and RS viruses in the pathology of caprine pneumonia across Nigeria. 150 goats were selected randomly. PI3 and RSV monoclonal antibodies and PPR polyclonal antibody were used for the immunolocalisation of the antigens. Histologically, 61 of the goats had broncho-interstitial pneumonia, 25 had interstitial pneumonia, 42 had bronchopneumonia, 12 had bronchiolitis and 10 were normal. PPR, PI3 and RS viral antigens were demonstrated in: intact and desquamated bronchial, bronchiolar epithelial cells, macrophages, leukocytes, pneumocytes and giant cells. 23% of the caprine lun...
Source: Journal of Immunoassay and Immunochemistry - July 6, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of a tree shrew-specific interferon-gamma assay.
Authors: Zhang X, Xu J, Wu Z, Zhu W, Dong S Abstract Tree shrews (Tupaia belangeri) are small squirrel-like mammals closely related to primates. Due to their susceptibility to several human viruses, tree shrews have been proposed as potential animal models for the study of human viral infections. However, there are no standardized assays currently available for the detection of tree shrew-specific interferon (IFN)-γ, a major cytokine secreted during the antiviral immune response. Herein, we developed a novel enzyme-linked immunosorbent assay (ELISA) for the quantification of IFN-γ in tree shrew serum sa...
Source: Journal of Immunoassay and Immunochemistry - July 6, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of the effect and mechanism of action of local phenytoin in treatment of vitiligo.
Authors: Abdou AG, Abdelwahed Gaber M, Elnaidany NF, Elnagar A Abstract There are many theories explaining vitiligo such as genetic, autoimmune, neural, free radicals, biochemical, intrinsic defect, melanocytorrhagy and convergent theories. Phenytoin is a widely used anticonvulsant, which is used in cutaneous medicine for treatment of ulcers and epidermolysis bullosa. The aim of this study is to assess the effectiveness of topical phenytoin gel in treatment of vitiligo patients and explaining the underlying mechanism using immunohistochemistry for evaluation of HMB45, CD4 and CD8. Only nine patients out of 28 exper...
Source: Journal of Immunoassay and Immunochemistry - June 25, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Innovative array-based assay for omics pattern in melanoma.
Authors: Dumitru C, Constantin C, Popp C, Cioplea M, Zurac S, Vassu T, Neagu M Abstract Cutaneous melanoma remains a major health issue and still an important challenge for research. Thus omics complex evaluation can provide a more specific molecular classification for this heterogeneous disease. Complex omics analysis based on genomic and proteomic microarrays can identify disease markers that prognosticate disease evolution or can monitor therapies efficacy. Among the technologies that gained momentum in the last years, array based comparative genomic hybridization offered the possibility to analyze chromosomal n...
Source: Journal of Immunoassay and Immunochemistry - June 16, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Measurement of the IgG2 response to Pneumococcal Capsular Polysaccharides may identify an antibody deficiency in individuals referred for immunological investigation.
Authors: Parker A, Irure Ventura J, Sims D, Echeverría de Carlos A, Gómez de la Torre R, Tricas Aizpún L, Ocejo-Vinyals JG, López-Hoyos M, Wallis G, Harding S Abstract IgG2 is the most efficient subclass for providing protection against pneumococcal pathogens. We hypothesised that some individuals may be unable to mount an effective pneumococcal capsular polysaccharide (PCP) IgG2 response despite having a normal PCP IgG concentration (PCP IgG2 deficient). The median pre-vaccination PCP IgG2 concentration was significantly lower in individuals referred for immunological investigation comp...
Source: Journal of Immunoassay and Immunochemistry - June 16, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Concentrations of Pneumococcal IgA and IgM are compromised in some individuals with antibody deficiencies.
Authors: Echeverría de Carlos A, Gómez de la Torre R, García Carus E, Caminal Montero L, Bernardino Díaz López J, Suárez Casado H, Molinos Matin L, Tricas Aizpún L, Harding S, Parker AR Abstract The response to pneumococcal vaccination is assessed by measurement of antigen specific IgG only and is compromised in a number of antibody deficiencies. We measured the concentrations of Pneumococcal IgA and IgM in individuals with both normal and abnormal pneumococcal capsular polysaccharide (PCP) IgG concentrations. A higher number of individuals had abnormal pre vaccinati...
Source: Journal of Immunoassay and Immunochemistry - June 16, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Neuroendocrine differentiation in basal cell carcinoma.
Authors: Houcine Y, Chelly I, Zehani A, Belhaj Kacem L, Azzouz H, Rekik W, C H, Haouet S, Kchir N Abstract Basal cell carcinoma (BCC) is the prototypical basaloid tumour of the skin. It may show various patterns simulating other cutaneous tumours due to its pleomorphism. It may have unusal pattern of differentiaton such as squamous, sebaceous, apocrine, eccrine, pilar and endocrine differentiation. In order to establish the relative frequency of neuroendocrine differentiation in BCC, we performed a retrospective study of 33 consecutive BCCs using conventional immunohistochemistry with two neuroendocrine antibodies:...
Source: Journal of Immunoassay and Immunochemistry - May 27, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Improvement of ELISA procedures through simultaneous addition of antigen and detection antibody and elimination of washing steps.
Authors: Gurevich V, Kotharu P, McCann K, Bertolini J Abstract The enzyme-linked immunosorbent assay (ELISA) is extensively used for measurement of proteins utilised for various research and diagnostic purposes in the biological disciplines. However it is a labour intensive and lengthy procedure due to a number of incubation and washing steps required for performing the assay. The ELISA procedure in the current study has been simplified through the simultaneous addition of antigen and detection antibody and elimination of washing steps. This resulted in a decreased time required to perform the procedure and without...
Source: Journal of Immunoassay and Immunochemistry - May 26, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Preparation and Development of Solid Phase Radioimmunoassay System for Determination of Cortisol.
Authors: Sallam KM Abstract The goal of the present work was oriented to prepare stable polystyrene coated tubes for direct radioimmunoassay (RIA) of human cortisol. Coating process was performed using rabbit polyclonal antisera specific for cortisol. The stability study showed that these tubes could be stored for up to one year at 4 ºC without any appreciable reduction in their binding. Labeling of cortisol was carried out using chloramin-T method and the (125)I-cortisol tracer was purified using HPLC column. Preparation of polyclonal antibodies (anti-cortisol) was carried out in host rabbit animals against c...
Source: Journal of Immunoassay and Immunochemistry - May 26, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Significance of pregnancy test false negative results due to elevated levels of β-core fragment hCG.
Significance of pregnancy test false negative results due to elevated levels of β-core fragment hCG. J Immunoassay Immunochem. 2017 May 19;: Authors: Johnson S, Eapen S, Smith P, Warren G, Zinaman M Abstract Very high levels of β-core fragment human chorionic gonadotrophin (βcf-hCG) are reported to potentially cause false negative results in point-of-care (POC)/over-the-counter (OTC) pregnancy tests. To investigate this further, women's daily early morning urine samples, collected prior to conception and during pregnancy, were analysed for intact, free β- and βcf-hCG. The propo...
Source: Journal of Immunoassay and Immunochemistry - May 20, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

An immunochromatographic assay for rapid detection of salvinorin a.
AN IMMUNOCHROMATOGRAPHIC ASSAY FOR RAPID DETECTION OF SALVINORIN A. J Immunoassay Immunochem. 2017 May 05;: Authors: Paudel MK, Shirota O, Sakamoto S, Morimoto S, Tanaka H Abstract We developed an immunochromatographic assay (ICA) that enables rapid analysis of salvinorin A (Sal A) in Salvia divinorum within 10 min. The result shows that no Sal A in other samples of Lamiaceae plants was detected, but it could recognize Sal A among other substances in complex samples. The main advantage of the ICA is its high performance in combination with low cost, simplicity, and speed. Our newly developed combined I...
Source: Journal of Immunoassay and Immunochemistry - May 7, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A novel in vitro ELISA for estimation of glycoprotein content in human rabies vaccines.
Authors: Aavula SM, Abhinay G, Nimmagadda SV, Maithal K Abstract In vitro methods for quantification of immunodominant glycoprotein in the rabies vaccine formulations serve as good alternative to the cumbersome and variable mice potency assay as a batch release test for the vaccine. The present study presents the development of a sandwich ELISA with optimal concentrations of a high affinity recombinant diabody (D06) and a specific monoclonal antibody (M5B4) against rabies glycoprotein for its quantification in the vaccine formulations. The glycoprotein estimate correlated linearly (r(2) = 0.8) to the in vivo potenc...
Source: Journal of Immunoassay and Immunochemistry - April 26, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Selfie: Autoimmunity.
Authors: Ahsan H Abstract The immune system provides protection to tissues damaged by infectious microrganisms or physical damage. In autoimmune diseases, the immune system recognizes and attacks its own tissues i.e., self-destruction. Various agents such as genetic factors and environmental triggers are thought to play a major role in the development of autoimmune diseases. A common feature of all autoimmune diseases is the presence of autoantibodies and inflammation, including mononuclear phagocytes, autoreactive T lymphocytes and autoantibody producing B cells (plasma cells). It has long been known that B cells ...
Source: Journal of Immunoassay and Immunochemistry - April 20, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

ALK protein expression in Pulmonary Adenocarcinoma of Tunisian patients.
CONCLUSION: The frequency of positive ALK expression based on immunohistochemistry in our series was similar to that reported in the world literature. PMID: 28421869 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - April 20, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of Two In-House Immunoenzymatic Tests to Serodiagnose Subclinical Paratuberculisis in a Sheep Flock in Mexicali Valley, Mexico.
In this study, two MAP antigens were compared for their diagnostic utility to detect subclinical PTB in a sheep flock in Mexicali, Mexico. Sheep (n = 31) without clinical signs but positive on a direct fecal-polymerase chain reaction were tested with two preabsorbed in-house enzyme linked immunosorbent assays (ELISAs) using: (1) an ethanol-extracted surface lipid antigen (EVELISA) and (2) a protoplasmic antigen (ELISA-PPA). Sensitivities of the EVELISA and ELISA-PPA were 84% (95% CI; 66-95%) and 29% (95% CI; 14-48%), respectively. The EVELISA test could be a fast and effective way to identify subclinical ovine PTB for seve...
Source: Journal of Immunoassay and Immunochemistry - April 20, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Ascorbic acid deficiency in patients with lichen planus.
CONCLUSIONS: The urinary ascorbic acid level may be a useful parameter in identifying patients with LP who are at risk of developing viral or bacterial infections. PMID: 28422565 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - April 20, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Myositis non-inflammatory mechanisms: An up-dated review.
Authors: Manole E, Bastian AE, Butoianu N, Goebel HH Abstract Idiopathic inflammatory myopathies (IIM) represent a heterogeneous group of rare muscular diseases, with no clearly known causes. IIM frequently have an incomplete response to treatment due to the difficulty in distinguishing between IIM forms, and due to neglect their non-inflammatory causes. Important data concerning non-immune mechanisms in IIM pathology have been recently accumulated. There is a correlation between inflammatory and non-inflammatory mechanisms, but their involvement in IIM pathogenesis is still unknown. Here we review some of the most...
Source: Journal of Immunoassay and Immunochemistry - April 6, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

High rate of Hepatitis B virus infection among hairdressers in Ibadan, Nigeria.
This study was designed to determine the levels of HBV awareness and prevalence of HBV infection among hairdressers in Ibadan, Nigeria. Hairdressers and teachers (unmatched controls) in four local government areas in Ibadan were tested for HBV infection using ELISA technique. Dried blood spot (DBS) samples were collected from 171 participants. DBS elutes from the samples were tested for HBV surface antigen (HBsAg). The rate of HBV infection was higher (p=0.005) among the hairdressers (13.0%) than teachers (6.0%). However, teachers were better informed about HBV (38%) compared to hairdressers (13%; p=0.0001). Differences in...
Source: Journal of Immunoassay and Immunochemistry - March 23, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Highlights of new immunoassay-based technologies.
Authors: Tanase C, Albulescu R, Neagu M PMID: 28102778 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - January 21, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Scale up Production and Characterization of Anti-Human Cardiac troponin I Monoclonal Antibody in Ascitic Fluid of Balb/c Mice.
Authors: Majid AR, Javad RM, Malihe P, Javad M Abstract The Human Cardiac Troponin I (hcTnI) is a 210 amino acid protein, 23 kDa in molecular weight. This biomarker is commonly used to diagnose myocardial infarction, micro injury, and acute coronary syndrome (ACS) in patients referring to emergency departments. The American Heart Association (AHA) and European Society of Cardiology (ESC) proposed troponin I as the gold biomarker for early detection of heart attack, especially in myocardial infarction (MI). Therefore, developing monoclonal antibodies against this biomarker could help in for early detection of heart ...
Source: Journal of Immunoassay and Immunochemistry - December 29, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody.
DEVELOPMENT OF AN IMMUNOASSAY USING AN ANTI-WOGONIN GLUCURONIDE MONOCLONAL ANTIBODY. J Immunoassay Immunochem. 2016 Dec 27;: Authors: Paudel MK, Sakamoto S, Huy LV, Tanaka H, Tomofumi M, Akihito T, Morimoto S Abstract Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn-bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactiv...
Source: Journal of Immunoassay and Immunochemistry - December 29, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of immune response in challenged chickens vaccinated with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agents.
Authors: Oyebanji VO, Emikpe BO, Oladele OA, Odeniyi MO, Salami A, Osowole OI, Kasali OB, Akinboade OA Abstract Immune response of challenged chickens following previous vaccinations with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agent were evaluated. Two hundred and fifty-two (252) one-day old chickens were divided into vaccine-gum oral (GVOR), vaccine-gum ocular (GVOC), vaccine oral (VOR), vaccine ocular (VOC), gum oral (GOR), gum ocular (GOC), No-gum-no-vaccine/challenged (NGNV/C) & No-gum-no-vaccine/unchallenged (NGNV/U) groups. They were vaccinated at days...
Source: Journal of Immunoassay and Immunochemistry - December 26, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A Comparison of Nanoparticle-Antibody Conjugation Strategies in Sandwich Immunoassays.
Authors: Tam JO, de Puig H, Yen CW, Bosch I, Gómez-Márquez J, Clavet C, Hamad-Schifferli K, Gehrke L Abstract Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate...
Source: Journal of Immunoassay and Immunochemistry - December 17, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Spontaneous mouse mammary tumor cell lysates induce IgG production in spleen mononuclear cells of healthy and tumor-bearing mice.
CONCLUSION: it seems that TCL prepared from SMMT are potent inducers of IgG production. This may propose TCL as a potential tool for monitoring of humoral immunity in animal models. PMID: 27929756 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - December 10, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Simultaneous Determination of Glycyrrhizin and Liquiritin in Licorice Roots and Kampo medicines by Combination Enzyme-linked Immunosorbent Assay using anti-Glycyrrhizin and anti-Liquiritin Monoclonal Antibodies.
In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines. PMID: 27892811 [PubMed - as supplied by publisher] (S...
Source: Journal of Immunoassay and Immunochemistry - November 30, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunoassay and molecular methods to investigate DNA methylation changes in peripheral blood mononuclear cells in HIV infected patients on cART.
This study aimed to investigate the influence of antiretroviral therapy on methylation markers, in a group of HIV infected, heavily treated patients. Immune and molecular methods were used to investigate potential changes in methylation profile in DNA isolated from peripheral blood mononuclear cells collected from antiretroviral-experienced HIV infected patients and healthy controls. The percentage of 5-methylcytosine was inversely correlated with proviral DNA and active replication while DNMT1 (p=0.01) and DNMT3A (p=0.004) independently correlated with active viral replication. DNMT3A expression increased with total treat...
Source: Journal of Immunoassay and Immunochemistry - November 19, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Measuring fecal testosterone metabolites in spotted hyenas: choosing the wrong assay may lead to erroneous results.
Authors: Pribbenow S, Shrivastav TG, Dehnhard M Abstract Enzyme-immunoassays (EIA) that detect fecal testosterone metabolites (fTM) are powerful tools to monitor gonadal activity non-invasively. However, a challenge with testosterone EIAs might be their potential for cross-reactivities with structurally similar glucocorticoid metabolites. Therefore, we aimed to verify the capability of four different testosterone EIAs to monitor fTM without reflecting changes in adrenocortical activity in spotted hyenas by analyzing fecal samples following testosterone and ACTH challenge tests. We demonstrated that none of the test...
Source: Journal of Immunoassay and Immunochemistry - November 19, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Comparison of two competitive enzyme immunoassay kits for quantification of plasma Urotensin-II in rats.
Authors: Rodríguez-Rodríguez A, José Egea-Guerrero J, Vilches-Arenas Á, Quintanilla-Vázquez MJ, Murillo-Cabezas F, Muñoz-Sánchez MÁ Abstract Changes in Urotensin-II (U-II) concentration, a potent vasoconstrictor peptide, have been detected in various pathologies, but it has been impossible to define a normality range. We aimed to analyze the concordance and interchangeability between two enzyme immunoassay methods developed by Phoenix Pharmaceuticals, Inc. to measure U-II plasma concentration in rats: ELISA and fluorescent EIA. Assays resulted positively correlated (r=0.850; p
Source: Journal of Immunoassay and Immunochemistry - November 3, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A Multi-Analyte Immunoassay for Thyroid Related Analytes.
We describe the development and validation of multianalyte immunoassays (MAIA) for three analytes, viz., thyroxine (T4), thyroid stimulating hormone (TSH) and thyroglobulin (Tg) essential for assessment of thyroid function but having widely varying molecular weights. Using polycarbonate (PC) track-etched membranes (TEM) as an immobilization support and (125)I as the tracer, both competitive assay for T4 and non-competitive assay for TSH and Tg were performed on the same TEM. MAIA was found to be highly sensitive and precise with clinically useful working range and correlated very well with individual analyte immunoassays. ...
Source: Journal of Immunoassay and Immunochemistry - November 3, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A New Covalent Peroxidase Conjugation Method Using Bis(Sulfosuccinimidyl) Suberate as Cross-linking Reagent, in a Two Step Procedure.
Authors: Rivo P Abstract A new method has been developed to prepare horseradish peroxidase (HRP) conjugates, using bis(sulfosuccinimidyl)suberate (BS3) as cross-linking reagent in a two-step procedure. The enzyme is reacted with BS3, introducing active ester molecules into the enzyme itself, and it is then used directly to label amino-containing compounds without further treatment. The proteins involved in the conjugation undergo minimal modifications. The reaction conditions are evaluated, as well as studies on the conservation of the biological activities of the conjugated proteins and the stability of the conjug...
Source: Journal of Immunoassay and Immunochemistry - November 2, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Eliciting an antibody response against a recombinant TSH containing fusion protein.
This study underscores the applicability of designed fusion protein for specific and sensitive polyclonal antibody production and the importance of selecting an amenable region of the TSH for immunization. PMID: 27786604 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - October 28, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Prevalence of anti-hepatitis C cirus antibody among pregnant women and blood donors at Bowen University Teaching Hospital, Ogbomoso, Oyo State, Nigeria.
This study was carried out to determine the prevalence of anti-HCV antibodies among potential blood donors and pregnant women attending Bowen University Teaching Hospital (BUTH), Ogbomoso, Oyo State. Hospital based study was conducted from December 2014 to September 2015. The study group (N = 279) included potential blood donors and pregnant women. Data on socio-demographic characteristics and potential risk factors were collected using a structured questionnaire. The presence of anti-HCV antibodies in serum samples of the studied subjects were detected using third generation Enzyme Linked Immunosorbent Assay (ELISA) (WKEA...
Source: Journal of Immunoassay and Immunochemistry - September 29, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Establishment of a reference ELISA for measurement of universal thresholds of pregnanediol glucuronide excretion rates using urine samples diluted to a constant volume per unit time.
Authors: Binnie JE, Cooke DG, Blackwell LF Abstract An enzyme linked immunosorbent assay (ELISA) for measurement of pregnanediol-3α-glucuronide (PdG) excretion rates in urine samples diluted to 150 mL/h before analysis is described. The sensitivity of the 9 optimized standard curves was 0.093±0.070 μmol PdG/24 h, with the multiple combined standard curves having a mean mid-point (EC50) of 6.88 μmol PdG/24 h. The PdG threshold excretion rate of 7.0 μmol/24 h,which is used as a marker for the end of fertility, was situated in the most accurate region of the standard curve. The specificity of the...
Source: Journal of Immunoassay and Immunochemistry - September 19, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

An enzyme immunoassay for determining epidemal growth factor (EGF) in human serum samples using an ultramicroanalytical system.
Authors: Castells Martínez EM, Del Valle R, González EC, Melchor A, Pérez PL, González I, Carr A, León K Abstract Human epidermal growth factor is a small peptide consisting of 53 amino acid residues, which stimulates cell proliferation and is associated with several human carcinomas. A simple sandwich-type ultramicroELISA assay (UMELISA), based on the advantages of high affinity reaction between streptavidin and biotin has been developed for the measurement of EGF in human serum samples. Strips coated with a high affinity monoclonal antibody directed against EGF are used as solid...
Source: Journal of Immunoassay and Immunochemistry - September 19, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

EOV Editorial Board.
Authors: PMID: 27636685 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - September 19, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Selection of specific inhibitor peptides in enzyme-linked immunosorbent assay (ELISA) of cardiac troponin i using immuno-dominant epitopes as competitor.
Authors: Asiabanha Rezaee M, Rasaee MJ, Mohammadnejad J Abstract Human cardiac troponin I (cTni) is the gold marker for early diagnosis of myocardial infarction. In this regard, four immune-dominant epitopes of cTni were predicted and their 3D structures were determined. Thereafter, the competitive performance of the peptides was monitored with the developed polyclonal antibody-based indirect competitive ELISA; a half-maximal inhibitory concentration (IC50) of 0.49 (µg/mL) and detection limit of 0.037 (µg/mL) were achieved for recombinant cTni. The competitive ELISA determined sensitivity levels of 0.30...
Source: Journal of Immunoassay and Immunochemistry - September 15, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

C3:CH50 ratio as a proposed composite marker for Eculizumab monitoring in atypical hemolytic uremic syndrome: Preliminary results.
Authors: Kerboua KE, Haiba F, Batouche D Abstract Treatment of atypical hemolytic uremic syndrome (aHUS) by the complement C5 inhibitor Eculizumab (Soliris(®)) is highly effective but unfortunately, associated with an economic pressure on the health care systems even in high incomes countries. Despite spacing infusions has been proposed as the unique solution to minimize this economic impact, no reliable laboratory assays are available to tailor such therapy optimization. To propose and evaluate a complement composite marker for eculizumab efficacy monitoring in aHUS. We have retrospectively analyzed complement...
Source: Journal of Immunoassay and Immunochemistry - September 14, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method.
Authors: Zeman D, Kušnierová P, Bojková J, Všianský F, Zapletalová O Abstract IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was obser...
Source: Journal of Immunoassay and Immunochemistry - September 11, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development and validation of an enzyme-linked immunosorbent assay for detection of cortisol in human saliva.
In conclusion, an enzyme immunoassay test was successfully produced, validated and used for cortisol measurement in saliva samples. PMID: 27600799 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - September 10, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Sialic acid level is significantly elevated in IgM enriched protein fraction in sera of cancer patients.
Authors: Torii T, Kanemitsu K, Hagiwara A Abstract Serum sialic acid (SA) levels are important for diagnosis, follow-up, and mechanistic analysis of malignant diseases. However, little is known about the levels of SA bound to serum IgM. Here, we isolated IgM from sera of healthy individuals and patients with cancer using DEAE chromatography and 8% polyethylene glycol precipitation. In this fraction, which contained partially purified IgM (recovery; 52%; purity: 25%), SA was quantified with fluorescence detection-HPLC (detection limit: 0.08 μM). SA levels in the IgM-enriched fraction was significantly higher in c...
Source: Journal of Immunoassay and Immunochemistry - September 10, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunomodulatory effects of turmeric: Proliferation of spleen cells in mice.
Authors: Mustafa R, Blumenthal E Abstract Experiments were conducted to examine the effects of turmeric on spleen cell proliferation. Cell suspensions of spleen cells from young and aged mice were treated with or without conconavalin A (Con-A) as a proliferation stimulant, and with and without turmeric (20 mg/ml) in different concentrations. Spleen cells from young mice that received turmeric showed significant increase in spleen cell proliferation (p
Source: Journal of Immunoassay and Immunochemistry - August 26, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development and application of dot-enzyme-linked immunosorbent (dot-ELISA) assay for detection of Brucella melitensis and evaluation of the shedding pattern in infected goats.
Authors: Onilude OM, Yusoff SM, Emikpe BO, Tanko P, Shahrom SM, Effendy M Abstract Early and accurate diagnosis of Brucella melitensis is essential for the treatment and control of brucellosis both in animals and humans. The thrust for the development of a rapid diagnostic technique to overcome the limitations of conventional microbiological and serological tests brought about this investigation on the development and application of dot-ELISA for antigen and antibody detection in infected goats. Fifteen apparently healthy Boer aged 2 - 3 years which tested negative for brucellosis using PCR and ELISA, were grouped ...
Source: Journal of Immunoassay and Immunochemistry - August 7, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The diagnostic importance of the bronchoalveolar lavage in lymphocytic alveolitis.
CONCLUSION: The relevance of both techniques depends on many factors. They necessitate an available material, well-trained technicians and experimented pathologists. PMID: 27471881 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - July 31, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Significance of immune response to Mycobacterium tuberculosis culture filtrate protein antigens in cerebrospinal fluid of tuberculous meningitis patients: A search for diagnostic marker.
Authors: Giribhattanavar P, Kumar K, Raajasekar S, Chandrashekar N, Patil S Abstract Mycobacterium tuberculosis (H37Ra) culture filtrate proteins (CFP) are explored as a diagnostic marker for tuberculous meningitis (TBM). Cerebrospinal fluid (CSF) samples from patients were categorized as confirmed (n=47), suspected (n=20) and non-TBM (n=25) cases. Immune response by western-blot revealed TBM CSF samples are having heterogeneous response to CFP. CFP ELISA was 92% sensitive and 38.30% specific. ODs of confirmed TBM and non-TBM cases were significantly different (P
Source: Journal of Immunoassay and Immunochemistry - July 29, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A quantitative enzyme-linked immunosorbent assay for quantification of secretory immunoglobulin A in serum.
Authors: Pérez-Griera J, Andreu-Ballester JC, Hueso Zarandieta A, García de la Asunción J, Masquefa Bondia S Abstract Secretory immunoglobulin A (SIgA) in serum is possibly the best index of SIgA presence in mucosal secretions in digestive tract and the mirror of its immunologic barrier against many pathogenic aggressions. The measurement of salivary SigA alone may be affected by total salivary secretion and its final concentration in the gland lumen is probably not useful as a appropriate index of mucosal secretions in the digestive tract. The usefulness of the determination of SigA against va...
Source: Journal of Immunoassay and Immunochemistry - July 29, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of recombinant matrix metalloproteinase-3 based sandwich ELISA for sero-diagnosis of canine mammary carcinomas.
Authors: Pandey M, Sunil Kumar BV, Singh S, Verma R Abstract Matrix metalloproteinase-3 is invariably upregulated in cancerous condition. So we aimed to determine serum level of MMP-3 in canine mammary tumors. The gene was expressed in E. coli system as ~43kDa recombinant protein, which was refolded, purified and confirmed. Hyperimmune serum was raised against the expressed protein in rabbits and mice to standardize sandwich ELISA. ROC analysis revealed largest area under the curve of 0.998 with sensitivity (100%) and specificity (95%) for a cut-off value of 0.363 with respect to histopathological staining. The fin...
Source: Journal of Immunoassay and Immunochemistry - July 14, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Silencing of epidermal growth factor, latrophilin and seven transmembrane domain-containing protein 1 (ELTD1) via siRNA induced cell death in glioblastoma.
In this study we aim to analyse whether this receptor may be used as a target molecule in glioblastoma therapy. Our results showed that small interfering RNA silencing ELTD1 caused cytotoxicity in glioblastoma cells. We also found that PDGFR, VEGFR and their common PI3K/mTOR intracellular pathway inactivation induced cytotoxicity in glioblastoma cells. Further, we found high percent of cytotoxicity in a low passage glioblastoma cell line after BEZ235 (a dual inhibitor of PI3K/mTOR pathway) treatment at nanomolar concentrations, compared to AG1433 (a PDGFR inhibitor) and SU1498 (a VEGFR inhibitor) that were only cytotoxic a...
Source: Journal of Immunoassay and Immunochemistry - July 6, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of a high sensitivity C-reactive protein immunoassay and comparison with a commercial kit.
This study aimed to develop a cost effective and high sensitivity CRP immunoassay for use in cardiac risk assessment. Assay was optimized for coating, blocking of capturing antibody, dilution & reaction time of the conjugate and sample volume. For normal reference range, CRP was determined in serum samples from apparently healthy volunteers. For clinical validation, CRP was determined in samples of acute coronary syndrome patients by in-house and commercial assays. The lower detection limit of in-house assay was 0.16 µg/L. Intra and inter assay imprecision was 4.39%, 4.6% and 8.6%, 9.3%, respectively. The correla...
Source: Journal of Immunoassay and Immunochemistry - July 3, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of a time efficient immunization strategy for anti-PAH antibody development.
Authors: Li X, Kaattari SL, Vogelbein MA, Unger MA Abstract The development of monoclonal antibodies (mAb) with affinity to small molecules can be a time-consuming process. To evaluate shortening the time for mAb production, we examined mouse antisera at different time points post-immunization to measure titer and to evaluate the affinity to the immunogen PBA (pyrene butyric acid). Fusions were also conducted temporally to evaluate antibody production success at various time periods. We produced anti-PBA antibodies 7 weeks post-immunization and selected for anti-PAH reactivity during the hybridoma screening process...
Source: Journal of Immunoassay and Immunochemistry - June 11, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

IL-27 plasma level in relapsing remitting multiple sclerosis subjects: The double-faced cytokine.
Authors: Naderi S, Hejazi Z, Shajarian M, Alsahebfosoul F, Etemadifar M, Sedaghat N Abstract Interleukin-27 (IL-27) is a member of IL-6/IL-12 cytokine family which possesses pro- and anti-inflammatory properties and participates in the pathogenesis of various autoimmune diseases. In our case-control study, plasma was collected from healthy subjects as control group (n=40) and patients with relapsing-remitting multiple sclerosis (RRMS) (n=40), including new identified cases without treatment (n=12) and those treated with Interferon beta (IFN-β) (n=28). The plasma level of IL-27 was assessed by ELISA method. Our...
Source: Journal of Immunoassay and Immunochemistry - June 2, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research