Effect of dietary status on plasma-fractionated metanephrines in healthy individuals measured by Elisa.
This study was conducted at the Chemical Pathology Laboratory, Royal Hospital, Oman. Three plasma samples were collected from each participant (16 male and 16 female) over three consecutive days (day one: dietary restriction,day two: excess intake of restricted foods, day three: random sample following the typical diet for each participant). Samples were collected and centrifuged to obtain the plasma, which was then stored at -20°C prior to analysis. Metanephrine and normetanephrine concentrations were measured by comparative ELISA. Plasma metanephrine and normetanephrine measured under the three different dietary cond...
Source: Journal of Immunoassay and Immunochemistry - June 19, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Effects of resveratrol on shedding and pathological dynamics in experimental B. melitensis infection in dexamethasone-treated nonpregnant Boer goats.
This study evaluated relationship between Brucella melitensis infection, shedding dynamics, dexamethasone-induced stress, pathological alterations and resveratrol ameliorative effects in goats. Twelve nonpregnant goats were divided into four groups A, B, C, and D of three animals each. Groups A and B were administered 107 CFU/mL of B. melitensis ocularly, 21 days prior to 7 days consecutive administration of dexamethasone (2 mg/kg). Group A was further administered resveratrol (5 mg/kg) intravenously for 5 consecutive days from day 31 post B. melitensis inoculation. Group C was administered similar dose of B...
Source: Journal of Immunoassay and Immunochemistry - June 5, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Singlet oxygen species and systemic lupus erythematosus: a brief review.
Authors: Ahmad R, Ahsan H Abstract Reactive oxygen species (ROS) are implicated in inflammatory, autoimmune, and neurodegenerative diseases. The activation, proliferation, and apoptosis of immune cells are dependent on the controlled production and elimination of ROS. However, under chronic inflammatory conditions, large amounts of ROS generated are a major cause of many human diseases. The electronically excited molecular oxygen species known as singlet oxygen (1O2) is a form of ROS and is one of the major cytotoxic species in eukaryotic cells. ROS are known to cause DNA damage leading to strand breaks, base damag...
Source: Journal of Immunoassay and Immunochemistry - May 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The investigation of relevancy between PIAS1 and PIAS2 gene expression and disease severity of multiple sclerosis.
This study aims to evaluate the gene expression of these factors in multiple sclerosis (MS) patients compared to healthy individuals and correlate them with the severity of MS. MATERIALS AND METHODS: Sixty participants, including 30 patients with MS and 30 healthy controls were studied. The expression of PIAS1 and PIAS2 genes in peripheral blood samples of all participants was measured by real-time PCR. The severity of MS was evaluated using the Expanded Disability Status Scale (EDSS). Finally, we evaluated the correlation between the expression of PIAS1 and PIAS2 genes with disease severity. RESULTS: The expressio...
Source: Journal of Immunoassay and Immunochemistry - May 16, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of monoclonal antibody-based enzyme-linked immunosorbent assay for quantitative quality control of Derris scandens (Roxb.) Benth.
Authors: Jutathis K, Pongkitwitoon B, Sritularak B, Tanaka H, Putalun W Abstract Derris scandens (Roxb.) Benth. is a medicinal plant used for treatment of musculoskeletal pain in Thai traditional medicines. Its stem contains active compound genistein-7-O-[α-rhamnopyranosyl-(1 to 6)-β-glucopyranoside] (GTG) which is used as a biomarker for standardization of D. scandens extracts. As an alternative for rapid quantitation of GTG, a monoclonal antibody against GTG was prepared and applied for an indirect competitive enzyme-linked immunosorbent assay (ELISA) to determine GTG in plants and herbal products. The...
Source: Journal of Immunoassay and Immunochemistry - May 16, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serological evidence of Rift Valley fever virus infection in slaughtered ruminants in Nigeria.
Authors: Opayele AV, Ndiana LA, Odaibo GN, Olaleye DO Abstract The risk of exposure of slaughterhouse workers to Rift Valley fever (RVF) virus-infected animals in Nigeria was assessed by determining the prevalence of anti-RVF IgM in cattle, goats and sheep slaughtered in a major abattoir in Ibadan, Nigeria. Blood samples were collected from 290 animals in Bodija Municipal abattoir, Ibadan, Nigeria in January and February 2017 and analyzed for the presence of RVF virus using IgM Enzyme-Linked Immunosorbent Assay (ELISA) and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) for detection of the virus RNA. Desc...
Source: Journal of Immunoassay and Immunochemistry - May 15, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The proportion-ratio on dipeptidyl aminopeptidase-4 (DP-4) inhibition by gelatin compared to synthetic sitagliptin.
Authors: Atma Y, Lioe HN, Prangdimurti E, Seftiono H, Taufik M, Fitriani D, Mustopa AZ Abstract The current study aims to determine the inhibition activity gelatin against dipeptidyl aminopeptidase 4 (DP-4). Two commercial gelatins, i.e., bovine and fish skin gelatin and one extracted (in our laboratory) gelatin, i.e., fish bone gelatin were selected for analysis. Each gelatin have same protein pattern (75-245 kDa) on sodium dodecyl sulfate polyacrylamide gel electrophoresis with mean of protein concentration of 1.72 mg/mL. The inhibition activity was measured on the capacity to inhibit DP-4 by using Gly-Pro-p...
Source: Journal of Immunoassay and Immunochemistry - May 10, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Generation and characterization of monoclonal antibodies against thyroid-stimulating hormone for newborn screening of congenital hypothyroidism.
Authors: García de la Rosa I, Feal Carballo S, Almenares Guasch PR, Segura González MT, Pupo Infante M, Frómeta Suárez A, Martínez Beltrán L, Quintana Guerra JM, Lafita Delfino Y, Morejón García G, Pérez Morás PL, Hernández Pérez L, Castells Martínez EM Abstract Congenital hypothyroidism (CH) is one of the most frequent inherited-metabolic diseases in the world, and the main cause of treatable mental retardation in children. Because signs and symptoms of this disease are often scarce and not easily recognizable, newborns are ...
Source: Journal of Immunoassay and Immunochemistry - May 5, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Tetracycline and ciprofloxacin multiresidues in beef and chicken meat samples using indirect competitive ELISA.
Authors: Baghani A, Mesdaghinia A, Rafieiyan M, Soltan Dallal MM, Douraghi M Abstract In livestock and poultry, broad-spectrum antibiotics such as tetracyclines and fluoroquinolones are widely used; thus, controlling food productions made from these animals is a necessary task. Meat may contain residues of antibiotics, even in low concentrations, which can cause a selection pressure for antibiotic resistance. Therefore, measurement of amounts of antibiotics in meat is of major importance. A total of 41 beef and 41 chicken meat samples were collected for 1 year. Tetracycline and ciprofloxacin were extracted from sam...
Source: Journal of Immunoassay and Immunochemistry - April 6, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development and validation of a noncompetitive electrochemiluminescence-based immunoassay (ECLIA) for specific determination of insulin lispro (Humalog ®) in human serum to support pharmacokinetic assessments.
We report herein development of the first noncompetitive electrochemiluminescence-based immunoassay (ECLIA) for specific determination of insulin lispro in serum or plasma. The new sandwich ECLIA permits accurate assessment of insulin lispro pharmacokinetics without interference from endogenous insulin. Integral to the development of this specific immunoassay was establishment of a proprietary process for affinity production of an oligoclonal monospecific guinea pig antiserum to the unique subtle structural modification in insulin lispro. We specifically optimized the ECLIA to provide reliable performance for supporting ph...
Source: Journal of Immunoassay and Immunochemistry - March 30, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Histological and immunohistochemical assessments of pneumonia in sheep slaughtered at Ibadan, Nigeria and Kumasi, Ghana.
This study described the histological pattern, immunohistochemically demonstrate bacterial and viral agents of pneumonia in sheep slaughtered in metropolitan abattoirs in Nigeria and Ghana. A total of 805 (600 in Ghana and 205 in Nigeria) sheep lungs were examined for pneumonic lesions. Sections were fixed in 10% formalin for histological and immunohistochemical analysis. Pneumonia of 8.7% was in Ghana and 13.3% in Nigeria. The histological findings included bronchopneumonia (16/70), broncho-interstitial pneumonia (9/70), interstitial pneumonia (9/70) and proliferative pneumonia (7/70). Ten (14.3%) cases were positive for ...
Source: Journal of Immunoassay and Immunochemistry - March 23, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Peroxynitrite: cellular pathology and implications in autoimmunity.
Authors: Ahmad R, Hussain A, Ahsan H Abstract In inflamed tissues, the reaction of nitric oxide and superoxide leads to the formation of an extremely reactive peroxynitrite (ONOO-), which is a well known oxidizing and nitrating agent that exhibits high reactivity at physiological pH. The peroxynitrite formed can attack a wide range of biomolecules via direct oxidative reactions or indirect radical-mediated mechanisms thus triggering cellular responses leading to cell signaling, oxidative injury, committing cells to necrosis or apoptosis. Cellular DNA is an important target for ONOO- attack, and can react with deoxy...
Source: Journal of Immunoassay and Immunochemistry - March 9, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Rifampicin-resistant tuberculosis among known HIV-infected patients in Oyo State, Nigeria.
In conclusion, strict compliance with the infection control measures is strongly advocated for to prevent further transmission of Mycobacterium tuberculosis to people living with HIV most of whom have their immune system already weakened. PMID: 30835618 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - March 7, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

About the diagnostic value of BAP-1 antibody in malignant pleural mesothelioma: a meta-analysis.
CONCLUSION: This meta-analysis revealed that BAP1 antibody should be associated with more sensitive antibodies in order to assess the diagnosis. PMID: 30810450 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - February 28, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Human papillomavirus in pregnant women at Bowen University Teaching Hospital, Ogbomoso, Nigeria.
CONCLUSION: Awareness and screening of pregnant women should be strengthened among health stakeholders for proper management. PMID: 30794040 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - February 24, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Efficacy of silver nanoparticles against multidrug resistant clinical Staphylococcus aureus isolates from Nigeria.
Conclusion: This study revealed safety and efficacy of AgNP against clinical MDR S. aureus isolates from Nigeria, using plasmid eviction and protease inhibition as mechanisms of action. PMID: 30696349 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - February 1, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Advanced approach to activity evaluation for released-active forms of antibodies to interferon-gamma by enzyme-linked immunoassay.
In this study, ELISA was utilized to examine the effect of RA forms of Abs to interferon gamma on the interaction between monoclonal anti-interferon gamma antibodies and human interferon gamma. The data showed that such RA forms of Abs are able to modulate the antibody interaction with interferon gamma, and it was suggested that the observed influence of RA forms of Abs on 'antibody-antigen' interaction could be used to analyze the activity of this kind of drugs. PMID: 30663507 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - January 22, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The use of multiplexing technology in the immunodiagnosis of infectious agents.
Authors: Engin ED Abstract Traditionally, definitive diagnosis of infectious diseases is made by cultivation of the causative agent, while various antigens and antibodies as biomarkers of various diseases are detected by commercially available ELISA kits. PCR has emerged as a major innovation that greatly accelerated the accumulation of genomic and transcriptomic data, yet it has also revolutionized microbial diagnostics by enabling the detection of pathogen nucleic acid. Despite the advantages of and vast experience in ELISA and PCR, the next generation research and diagnostic tools have to fulfill the requirement...
Source: Journal of Immunoassay and Immunochemistry - January 22, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Multiplex assay for multiomics advances in personalized-precision medicine.
Authors: Popa ML, Albulescu R, Neagu M, Hinescu ME, Tanase C Abstract Building the future of precision medicine is the main focus in cancer domain. Clinical trials are moving toward an array of studies that are more adapted to precision medicine. In this domain, there is an enhanced need for biomarkers, monitoring devices, and data-analysis methods. Omics profiling using whole genome, epigenome, transcriptome, proteome, and metabolome can offer detailed information of the human body in an integrative manner. Omes profiles reflect more accurately real-time physiological status. Personalized omics analyses both disea...
Source: Journal of Immunoassay and Immunochemistry - January 12, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Updates in immune-based multiplex assays.
Authors: Tanase C, Albulescu R, Neagu M PMID: 30632893 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - January 12, 2019 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The biomolecules of beauty: biochemical pharmacology and immunotoxicology of cosmeceuticals.
Authors: Ahsan H Abstract Many ingredients in cosmetic products help to develop complex formulations that improve the quality of life in terms of disease prevention, health maintenance, beauty enhancement, and building self-esteem. The beneficial effects promoted from the use of biomolecule-rich substances into the formulations of various topical application products are considered useful ingredients in cosmetic and therapeutic applications. This review article attempts to understand the various biomolecules found in cosmetic products, particularly macromolecules, which may have an important role in prevention or p...
Source: Journal of Immunoassay and Immunochemistry - December 28, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serological profile of HBV infection among apparently healthy hospital attendees.
In conclusion, this work depicted various serological patterns of HBV infection among apparently healthy hospital attendees suggesting there are occult HBV infection and immune escape mutant in our population. It also showed that a huge number of the study population were susceptible to HBV infection. PMID: 30569812 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - December 22, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Human papillomaviruses' proteins with clinical utility.
Authors: Fudulu A, Albulescu A, Anton G Abstract Cervical cancer, the fourth leading cause of cancer-associated deaths among women worldwide, is associated with human papilloma virus (HPV) infection. Despite the prophylactic HPV vaccination and the implementation of cervical and HPV-based screening programs, a significant increase in cervical cancer incidence is estimated by the year 2020. Thus, further development of diagnostic tools that allow detection and risk assesment in genital HPV infection is necessary. A special interest is focused on the HPV viral proteins whose expression might be of use either as prima...
Source: Journal of Immunoassay and Immunochemistry - December 8, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Biomolecules and biomarkers in oral cavity: bioassays and immunopathology.
Authors: Ahsan H Abstract The oral mucosa protects the host against invading antigens and pathogenic microorganisms and contains an elaborate immune system and remains in a relative state of health despite the heavy antigen load. The oral barrier is exposed to unique and diverse communities of commensal microbial communities that are known to play immune-stimulatory roles in oral inflammatory diseases. Saliva is secreted from the salivary glands and has multiple functions, including mouth cleaning and protection, antibacterial effects, and digestion. The major protective function of salivary secretions in the oral ...
Source: Journal of Immunoassay and Immunochemistry - December 2, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Assessment of efficacy of dendritic cell therapy and viral therapy in high grade glioma clinical trials. A meta-analytic review.
Authors: Vatu BI, Artene SA, Staicu AG, Turcu-Stiolica A, Folcuti C, Dragoi A, Cioc C, Baloi SC, Tataranu LG, Silosi C, Dricu A Abstract In recent years, immunotherapy has raised the interest of many studies and provided different perspectives for the therapeutic management of high grade glioma. Our meta-analysis focused on the effectiveness of dendritic cell (DC) therapy and viral therapy (VT) in clinical trials. Fourteen eligible studies have been evaluated and the results suggest the improvement of both OS (HR = 0.65) (p
Source: Journal of Immunoassay and Immunochemistry - December 2, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A competitive ELISA for the quantitative determination of the novel anti-HIV drug candidate CIGB-210 in biological fluids.
In this study, we describe the development of a competitive ELISA for the quantitative determination of CIGB-210 using an anti-CIGB-210 hyperimmune serum. After optimization of all the steps, the assay exhibited a dynamic range from 11.87 to 0.0095 µg/mL. The intra-assay coefficient of variation (CV) was lower than or close to 5% for all the six concentrations of the calibrator, and the inter-assay CV was below 10% in five out of the six concentrations tested. No interference of either murine or human plasma was observed. The analyte was stable in plasma after five freeze-thaw cycles, while the hyperimmune serum...
Source: Journal of Immunoassay and Immunochemistry - December 2, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Protein-G-based lateral flow assay for rapid serodiagnosis of brucellosis in domesticated animals.
This study was conducted to develop and evaluate protein-G-based lateral flow assay (LFA) for rapid serodiagnosis of brucellosis in various domesticated animal species. The assay diagnostic performance was tested with 144 reference and 356 field sera samples and then compared with other serological assays. Results revealed that LFA showed 89% and 99% sensitivity and specificity, respectively, when compared with competitive ELISA as the gold standard. This study demonstrated LFA alone as a potential serodiagnostic assay for rapid serodiagnosis of brucellosis in various domesticated animal species. PMID: 30477402 [PubMed...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroepidemiological study of cystic echinococcosis in nomadic communities in the southwest of Iran: A population-based study.
Authors: Moshfe A, Sarkari B, Arefkhah N, Nikbakht R, Shahriarirad R, Rezaei Z, Jamshidi A, Moradian F Abstract The current study aimed at the seroepidemiological survey of human cystic echinococcosis (CE) in nomadic people in Boyer-Ahmad District in the southwest of Iran. One thousand and five nomads were selected by cross-sectional sample collection in nomadic tribes of Boyer-Ahmad District in the southwest of Iran. Blood samples were taken from each individual, and the sera were tested for detection of anti-hydatid cyst antibodies using antigen B-ELISA. A predesigned questionnaire which contained basic epidemiol...
Source: Journal of Immunoassay and Immunochemistry - November 23, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Augmentation of DTH reaction of mycobacterial antigenic cocktail using synthetic mycobacterial 19-kDa lipoprotein as a TLR-stimulant.
Authors: Mohamed AM, Ashshi AM, Abou El-Ella GA, Basalamah MA, Alandiyjany MN, Alsaegh AA Abstract The current study proposed that previously characterized individual antigenic proteins could represent potential replacement for conventional purified protein derivative (PPD) in tuberculosis skin testing when used in cocktails triggered by suitable TLR-stimulants that would provide the missing pro-inflammatory stimulus. Three different cocktails of previously selected antigens, including C1 (ESAT-6/CPF-10/MPB-83); C2 (ESAT-6/MPB-64/MPB-83); and C3 (CPF-10/MPB-64/MPB-83), were evaluated in vitro using lymphocytic prol...
Source: Journal of Immunoassay and Immunochemistry - November 21, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Preparation allergenic pollen extracts; the points should be considered to make high-quality products.
Authors: Ariaee N, Varasteh A, Jabbari Azad F, Sankian M Abstract Atopic diseases have an increasing trend worldwide during the last two decades. Determining the main cause of allergic diseases, allergens, is the first step in managing and improving the issue, usually is done by Skin Prick tests (SPTs). Having allergenic extract in high quality is desired to perform a reliable SPT. Several parameters of extracts are considered including composition, stability, potency, preservation conditions, and unit definition. In this review, these factors have been explained pointing to factors might have profitable points or ...
Source: Journal of Immunoassay and Immunochemistry - November 15, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Advances in immunosensors for clinical applications.
Authors: Balahura LR, Stefan-Van Staden RI, Van Staden JF, Aboul-Enein HY Abstract Immunoassay technique performs a fast, simple, reliable, and sensitive analysis of different compounds, being applied in several areas of interest such as clinical analysis for medical diagnosis, as well as in environmental analysis, and food quality control. The latest research activities in this field are represented by the attempts to achieve a low limit of detection by developing of new signal amplification strategies, eliminate the interferences, and decrease the cost of analysis. PMID: 30404580 [PubMed - as supplied by publ...
Source: Journal of Immunoassay and Immunochemistry - November 9, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Rift valley fever virus infection among livestock handlers in Ibadan, Nigeria.
This study indicates that RVF virus continues to circulate sub-clinically in Nigeria, thus the need to educate and vaccinate individuals who are occupationally at risk of infection. PMID: 30362911 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - October 27, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of reliability and performance of hepatitis B virus-e-antigen assays in tertiary care setting.
Authors: Fletcher GJ, Anantharam R, Radhakrishnan K, Singh U, Karunakaran A, Jeyaseelan V, Abraham P Abstract Hepatitis B virus-e-antigen (HBeAg) is a viral marker to assess hepatitis B virus (HBV) replication. We have evaluated the reliability of three commonly available HBeAg immunoassays using World Health Organization-International Standard and clinical samples. In addition the performance of enzyme immunoassays (EIAs) was assessed by kinetic binding and reagent exchange experiments. Analytical and diagnostic sensitivity were significantly different among HBeAg assays (P 
Source: Journal of Immunoassay and Immunochemistry - October 27, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Amino acid arginine and adducts: autoimmune activity.
Authors: Ahsan H Abstract Arginine is derived from dietary intake, body protein breakdown, or endogenous de novo arginine production. Arginine methylation of non-histone proteins is used in transcriptional regulation. Protein-arginine methylation is used for regulation of transcriptional and various physiological pathological processes. Protein methylation may affect protein-protein, protein-DNA, or protein-RNA interaction. Arginine has an effect on the DNA-binding activity of NF-κB, a dominant transcriptional factor in inflammation. Adduct formation results in increased secretion of messenger molecules such ...
Source: Journal of Immunoassay and Immunochemistry - October 27, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Type IV chitinase quantification in four different grape cultivars (Vitis vinifera) in northeast of Iran by an indirect sandwich enzyme-linked immunosorbent assay.
Authors: Farshidi N, Moghaddam M, Yaghoubi V, Ayati SH, Varasteh AR, Sankian M Abstract The incidence of grape (Vitis vinifera) allergy in the northeast of Iran is second to melon allergy. Type IV chitinase is one of the major grape allergens. The current study investigates the level of type IV chitinase in four grape variants for the first time in Khorasan Razavi Province using a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA). This assay was developed using a polyclonal antibody as a capture antibody and monoclonal antibody as a secondary one. Finally, the amount of type IV chitinase was measu...
Source: Journal of Immunoassay and Immunochemistry - October 23, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

"Application of the ultra micro analytical system (SUMA) technology for the detection of urinary hCG in antidoping control".
"Application of the ultra micro analytical system (SUMA) technology for the detection of urinary hCG in antidoping control". J Immunoassay Immunochem. 2018 Oct 22;:1-15 Authors: Martinez Brito D, Bueno Fis E, Fiallo Fernandez T, Torres Castellanos M, Correa Vidal MT, Montes De Oca Porto R Abstract The Ultra Micro Analytical System (SUMA) is an ELISA-based analytical platform, developed andmanufactured by the Cuban Immunoassay Center (IC), which is primarily used in clinical medicine applications. In this article, we describe the validation of the UMELISA HCG kits, which are based on SUMA, as ...
Source: Journal of Immunoassay and Immunochemistry - October 23, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Association of anti-RNP with HLADR4 and its prevalence in rheumatoid arthritis patients.
Authors: Mohammed Yousef A, Abdelrahman SK, Elsayed Helmi I, Mohammed Eltaweel F, Elsayed Hamed W Abstract BACKGROUND: Autoantibodies are useful diagnostic tools for a variety of rheumatic autoimmune disorders. OBJECTIVE: The aim of the present study was to investigate autoantibodies against the 70-kD polypeptide of U1 ribonucleoprotein antibodies (RNP) in patients with rheumatoid arthritis (RA) and to investigate the relationship between anti-RNP and HLARD4 in RA patients. PATIENTS AND METHODS: In this prospective study, we studied 80 RA patients. The patients were classified according to 2010 ACR/EULAR Rh...
Source: Journal of Immunoassay and Immunochemistry - October 19, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Neuroimmune cross-talk in Helicobacter pylori infection: analysis of substance P and vasoactive intestinal peptide expression in gastric enteric nervous system.
Authors: Sticlaru L, St─âniceanu F, Cioplea M, Nichita L, Bastian A, Micu G, Popp C Abstract It is suggested that different neuropeptides are actively involved in the pathogenesis of Helicobacter pylori (H. pylori)-induced gastritis acting as important effectors of the neuroimmune complex interactions, but the available data is limited and contradictory. The aim of this study was to determine whether the chronic infection generates changes in substance P (SP) and vasoactive intestinal peptide (VIP) gastric level and to evaluate the dependence of these potential effects on the degree of bacterial colonization or the...
Source: Journal of Immunoassay and Immunochemistry - October 17, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Improvement of recombinant-truncated Burkholderia motility protein A (BimA)-based indirect ELISA for equine glanders.
Authors: Singh S, Dohre SK, Kamthan A, Pal V, Karothia BS, Singha HS, Kumar S Abstract Glanders is a contagious and highly fatal disease of equines with zoonotic potential. It is caused by a Gram-negative, nonmotile bacterium Burkholderia mallei. Complement fixation test (CFT) is one of the most commonly used tests for diagnosis of glanders; however, it has some limitations. A recombinant-truncated Burkholderia intracellular motility A (BimA) protein-based indirect enzyme-linked immunosorbent assay (iELISA) was previously reported by us for glanders diagnosis, which has been re-optimized in this study using a panel...
Source: Journal of Immunoassay and Immunochemistry - October 13, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Mean platelet volume and neutrophil to lymphocyte ratio as parameters to indicate the severity of suicide attempt.
This study was designed to compare the complete blood count (CBC) values of 38 violent suicidal attempt (VSA) and 38 nonviolent suicidal attempt (NVSA) patients and 38 healthy controls, particularly mean platelet volume (MPV) and neutrophil to lymphocyte ratio (NLR). MPV was found to be significantly higher (P = 0.000), and PLT was found to be significantly lower in the VSA group (P = 0.001). NLR was found to be significantly higher (P = 0.005) in the VSA group. The area under the receiver operating characteristic curve of MPV levels for VSA was 0.78 and 0.68 for NLR in the VSA group when...
Source: Journal of Immunoassay and Immunochemistry - October 13, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Human albumin purification: a modified and concise method.
CONCLUSION: This method can be a robust technique for purification of albumin in order to use clinical and research approaches. PMID: 30311836 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - October 13, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

HIV and tuberculosis co-infection in a highly HIV-infected population of rivers state, Nigeria.
This study sought to determine the prevalence of TB among HIV-positive attendees of the Directly Observed Treatment of Short Course (DOTS) program at the military hospital in Port Harcourt. The association of HIV prevalence with gender and age as risk factors was also determined. A total of 200 patients aged 15-65 years were screened. Re-screening for HIV was done for confirmation. The overall prevalence for anti-TB antibodies amongst HIV-positive individuals was 14.0% (7.5% in males and 6.5% in females) while HIV prevalence following re-screening was 92%. It was observed that based on employment status and level of e...
Source: Journal of Immunoassay and Immunochemistry - October 10, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serum level of interleukin 36 in patients with multiple sclerosis.
Authors: Alsahebfosoul F, Jahanbani-Ardakani H, Ghavimi R, Sedaghat N, Etemadifar M Abstract INTRODUCTION: Multiple sclerosis is a chronic autoimmune demyelinating disorder of central nervous system with unknown origin. In MS disease, T cells are pointed to myelin antigens and it leads to myelin loss and axonal degeneration. Cytokines are important regulators of immune system and has critical roles in MS pathogenesis. Interleukin 36, a member of interleukin 1 family, has been shown having important roles in some autoimmune disorders due to its proinflammatory actions and its role in host immunity. METHODS AND M...
Source: Journal of Immunoassay and Immunochemistry - September 27, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Hepatitis E Virus infection seroprevalence and the associated risk factors in animals raised in Ibadan, Nigeria.
This study therefore investigates the seroprevalence and associated risk factors of HEV infection in pigs raised in Ibadan, Nigeria. Taking an analytical cross-sectional study design, 176 animals (comprising 120 pigs, 26 goats, and 30 cattle) were randomly sampled at the Bodija Municipal abattoir, Ibadan. Serum samples and demographic information were collected for HEV antibody detection (using a commercial recombinant genotype-3 antigen ELISA kit) and risk factors, respectively. A 57.5% (69/120) HEV seroprevalence was reported in the pigs while 0% prevalence was reported in the goats and cattle. In the pigs, a significant...
Source: Journal of Immunoassay and Immunochemistry - September 15, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immuno-analytical approach and its application for cardiac disease marker detection.
Authors: El-Kased RF Abstract Cardiac troponin-I is a promising diagnostic marker for cardiovascular diseases. Troponin-I immunoassays rely on monoclonal antibodies, while polyclonal antibodies, cheaper to manufacture, are uncommonly used. The current study established an immuno-analytical assay using a polyclonal antibody capable of mapping troponin-I antigenic determinant. Proteolytic digestion of troponin-I was performed. Antigenic determinant was assigned by separation of fragments using gel electrophoresis followed by Western blot and high-performance liquid chromatography followed by dot blot. The antigenic d...
Source: Journal of Immunoassay and Immunochemistry - September 15, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Antibodies against human parvovirus B19 in pregnant women in Ogbomoso, Oyo State Nigeria.
This study was carried out to determine the incidence of specific IgM antibodies to HPVB19 among pregnant women attending Bowen University Teaching Hospital, Ogbomoso, Oyo State. A total of 185 participants, in the age range 18-49 years, who consented voluntarily after thorough explanation of the purpose of the study were recruited for the study. Specific IgM antibodies were detected using commercially available third-generation ELISA kits. Data on socio-demographic characteristics and potential risk factors were collected using structured questionnaires. Chi-square (χ2) test was utilized to assess the association...
Source: Journal of Immunoassay and Immunochemistry - September 15, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A brief review of monoclonal antibody technology and its representative applications in immunoassays.
Authors: Gao Y, Huang X, Zhu Y, Lv Z Abstract Modern immunoassay methods and techniques are important tools in labs of basic biology, biomedicine, clinical medicine, and even in home tests, such as pregnant test, many of which utilize antibody-antigen binding mechanism as their foundational principle in common. Meanwhile, compared with polyclonal anitbody, monoclonal antibody shows obvious advantages in their application of modern immunoassays. Furthermore, the progress of other technologies have also promoted the development of modern immunoassays robustly, and widely made it extend into more research and industry...
Source: Journal of Immunoassay and Immunochemistry - September 13, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of the diagnostic value of emerin and CD56 in papillary thyroid carcinoma - an immunohistochemical study.
This study was performed on 50 cases of PTC, 9 cases of follicular adenoma (FA), and 12 cases of nonneoplastic thyroid lesions using immunohistochemistry for detection of emerin and CD56. Positive emerin expression was seen in 82% of PTC and in 16.7% of nonneoplastic cases with an absence of expression in FA. CD56 was expressed in 88.9% of FA, 91.7% of nonneoplastic cases and in a minority of PTC cases (6%). Positive emerin revealed 82% sensitivity and 90% specificity, while emerin-highlighted nuclear changes was more specific (95%). Negative CD56 expression revealed 84% sensitivity and 90% specificity. Combined positive e...
Source: Journal of Immunoassay and Immunochemistry - September 7, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Use of hafnium(IV) oxide in biosensors.
Authors: Ortiz-Dosal LC, Ángeles-Robles G, Kolosovas-Machuca ES Abstract Hafnium(IV) oxide is a material with properties that can increase the sensitivity, durability, and reliability of biosensors made from silicon dioxide and other semiconductor materials due to its high dielectric constant, thermodynamic stability, and the simplicity with which it can be deposited. This work describes the use of this material in biosensors based on field-effect transistors to detect ions and DNA, in immunosensors to detect an antigen-antibody complex, its use as a contrast material in computed tomography scans and the pos...
Source: Journal of Immunoassay and Immunochemistry - September 7, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Relationship between Th1 and Th2 cytokine serum levels and immune response to Hepatitis B vaccination among Egyptian health care workers.
Authors: Sabry R, Mohamed ZAZ, Abdallah AM Abstract Hepatitis B virus (HBV) vaccination leads to both humoral and cellular immune responses and results in protecting levels of specific antibodies. The immune response to the vaccine and production of T-helper 1 (Th1) and Th2 cytokines vary among the individuals. Thus we aimed to investigate the relationship between Th1/Th2 cytokine serum levels and antibody production after HBV vaccination, hoping to improve the effectiveness of vaccination. A total of 90 health care workers had been vaccinated with triple doses of HB vaccine at 0, 1, and 6 months intervals. Tw...
Source: Journal of Immunoassay and Immunochemistry - September 6, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research