Development of a western blot method for detection of fish ectoparasite Argulus siamensis antigens.
Authors: Das P, Mohanty J, Badhe MR, Sahoo PK, Sardar KK, Parija SC Abstract A study was undertaken to develop a western blot method for detection of immunogenic proteins of fish ectoparasite, Argulus siamensis for its further use as potential vaccine candidates. Argulus antigens were prepared by homogenization and injected to rohu (Labeo rohita) juveniles for development of immune serum. The serum was used to immunostain the antigens in western blot. The other reagents added in sequence were rabbit anti-rohu serum, goat anti-rabbit ALP conjugate and substrate (BCIP-NBT). However, similar banding patterns were obse...
Source: Journal of Immunoassay and Immunochemistry - August 14, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Differential expression of CCL18 in moderate/severe and mild persistent allergic rhinitis and its correlation with disease parameters.
CONCLUSION: The results of the study indicate that there could be a relationship between the expression of CCL-18 in nasal turbinate mucosa and the severity of allergic rhinitis. PMID: 30102123 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - August 14, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

High immunological response against a Trypanosoma equiperdum protein that exhibits homology with the regulatory subunits of mammalian cAMP-dependent protein kinases.
Authors: Mendoza E, Bubis J, Pérez-Rojas Y, Montilla AJ, Spencer LM, Bustamante F, Martínez JC Abstract Previously, we have identified a protein in Trypanosoma equiperdum that possesses homology with the regulatory (R) subunits of the mammalian cAMP-dependent protein kinase (PKA). The recombinant T. equiperdum PKA R-like protein was expressed in bacteria and purified to homogeneity. Mice polyclonal antibodies were raised against the recombinant R-like protein to serologically evaluate its humoral immune response. High titers of specific sera antibodies were obtained against the parasite R-like protein...
Source: Journal of Immunoassay and Immunochemistry - August 8, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serological detection of human T-cell lymphotropic virus types I and II among pregnant women in Ilorin, Nigeria.
This study aims at determining the prevalence of HTLV infection among pregnant women in Ilorin North-central Nigeria. Serum samples from 276 pregnant women who were antenatal clinic attendees at General and Civil Service Hospitals in Ilorin were tested for the presence of HTLV-I/II antibodies using Enzyme Linked Immunosorbent Assay test kits from Diagnostic Automation INC., USA. Out of the 276 women tested, 3 tested positive giving a prevalence rate of 1.1%. The result was analyzed on the basis of age, marital status, nature of family, educational status, occupation, religion, parity, and gestational stage of the women. Th...
Source: Journal of Immunoassay and Immunochemistry - August 1, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Use of chicken immunoglobulin Y in general virology.
This article reviews the latest IgY applications in diagnostic virology and the therapeutic use of IgY in viral gastroenteritis. PMID: 30044696 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - July 28, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Transmission transmissible hepatitis B virus markers of infection among sickle cell disease patients receiving care at a tertiary health facility in Ibadan, southwest Nigeria.
CONCLUSION: This study reported high rates of HBV markers of infection among SCD patients. It is therefore advocated that donated blood must pass through rigorous screening processes before it is transfused. PMID: 30024816 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - July 21, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Investigation of mother-to-child transmission of HIV in pregnancy and among HIV-exposed infants accessing care at a PMTCT clinic in southwest Nigeria.
CONCLUSIONS: There was a high risk of MTCT of HIV among exposed infants on follow-up at the PMTCT clinic of Adeoyo Maternity Teaching referral hospital. Our findings will assist health policy makers in providing important information capable of enhancing assurance HIV control in such population and in raising the standard of PMTCT program in Nigeria. PMID: 30001188 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - July 14, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of Fe3O4/Ag core/shell-based multifunctional immunomagnetic nanoparticles for isolation and detection of CD34+ stem cells.
Authors: Quynh LM, Dung CT, Mai BT, Huy HV, Loc NQ, Hoa NQ, Thach PT, Anh BV, Thao CT, Nam NH, Nhung HTM, Long NN, Vu LV Abstract Fe3O4/Ag core/shell nanoparticles functionalized with the free amino (NH2) functional groups (Fe3O4/Ag-NH2) were conjugated with fluorescent electron coupled dye (ECD)-antiCD34 antibody using the 1-ethyl-3-(3'-dimethyl-aminopropyl) carbodiimide (EDC) catalyst (ECD - Electron Coupled Dye or R Phycoerythrin-Texas Red is a fluorescent organic dye attached to the antibody). The characteristic fluorescence of ECD in the antibody was investigated and was used as a good indicator for estim...
Source: Journal of Immunoassay and Immunochemistry - July 12, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Human papillomavirus infection among women attending family planning clinic in Nigeria: prevalence, correlates, and co-infection with Chlamydia trachomatis.
Authors: Fowotade A, Osisanwo DA, Bakare RA Abstract Infection with high-risk genotypes of human papillomavirus (HPV) is considered the main cause of invasive cervical. A number of epidemiologic studies have suggested that HPV and Chlamydia trachomatis (CT) play a synergistic role in the etiology of cervical intraepithelial neoplasia and subsequent cervical cancer. The current study aimed to evaluate the HPV prevalence and the risk factors for co-infection with CT among women attending family planning clinic in Nigeria. Following enrolment, 90 patients were screened for IgG antibodies to virus-like proteins of HPV ...
Source: Journal of Immunoassay and Immunochemistry - July 12, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart's and Hb Portland 1.
In this study, we have produced polyclonal antibody (pAb) against HbF and established a modified sandwich-type ELISA for HbF quantification in blood lysates. The modified sandwich ELISA utilized anti-γ-globin monoclonal antibody clones Thal N/B as the capture antibody (Ab) coated on solid-phase, fluorescein isothiocyanate (FITC)-labeled pAb as the detecting Ab, and HPR-labeled anti-FITC Ab as the signal-generating Ab. By using an optimized blood lysate dilution, the HbF could be measured with no interference from hemoglobin Bart's (Hb Bart's) and hemoglobin Portland (Hb Portland 1) presented in α-thalassemia ca...
Source: Journal of Immunoassay and Immunochemistry - July 11, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Role of myeloperoxidase in early diagnosis of acute myocardial infarction in patients admitted with chest pain.
This study aimed to evaluate the diagnostic power of MPO in AMI patients. MPO, creatine kinase (CK) MB, and Troponin I (cTn I) were performed for all study patients. Area under the curves (AUCs) and 95% confidence intervals (CI); P values of baseline levels of MPO for discriminating AMI patients from noncoronary chest pain (NCCP) patients, stable angina (SA) patients, and unstable angina (UA) patients were 0.91, 95% CI: 0.82-0.99; P 
Source: Journal of Immunoassay and Immunochemistry - July 11, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A simple and low-cost portable paper-based ABO blood typing device for point-of-care testing.
Authors: Songjaroen T, Primpray V, Manosarn T, Khumchanta W, Sakuldamrongpanich T, Kulkeratiyut S, Laiwattanapaisal W Abstract ABO blood group is the most important blood type system for transfusion medicine. A paper-based analytical device (PAD) for ABO blood typing has been proposed. The device was composed of Whatman No. 113 paper, an absorbent gel pad, and a 3D-printing cassette. The 3D-printing cassette contained two circular holes for display of letters "A" and "B" on the PAD. Whole blood was dropped onto hydrophilic letters A and B on the PAD, in which the anti-A and anti-B were pre-immob...
Source: Journal of Immunoassay and Immunochemistry - June 30, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Relationship between immune cells and alcohol dependents and controls: what about the lymphocyte-related ratios?
Authors: Orum MH, Kara MZ, Egilmez OB PMID: 29944453 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - June 28, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Current methods for tumor-associated macrophages investigation.
Authors: Grigore A, Albulescu A, Albulescu R Abstract Oncoimmunology is a rapidly growing field, focusing both on studying of the interaction of immune factors with tumor cells and also on the development of new therapies. In this regard, immunotherapy is increasingly used clinically. Although tumorigenesis is generally seen as an autonomous process involving genetically transformed cancer cells, it is increasingly recognized that tumor environment is an essential factor that modulates both tumor progression and resistance to therapy. Tumor-associated immune cells, and in particular macrophages, are of particular i...
Source: Journal of Immunoassay and Immunochemistry - June 28, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Inhibition of LPS-induced inflammatory responses by Satureja hortensis extracts in J774.1 macrophages.
Authors: Farzaneh Z, Kalantar K, Iraji A, Amirghofran Z Abstract Macrophages are among the main cells involved in generation of inflammation. To investigate the anti-inflammatory effect of Satureja hortensis (summer savory), lipopolysaccharide (LPS)-activated J774.1 macrophages were treated with various extracts, and the expression and release of various inflammatory molecules by macrophages were examined. We showed that dichloromethane and hexane extracts reduced nitric oxide (NO) production more efficiently than other extracts. Both extracts decreased gene expression of inducible NO synthase (iNOS) (
Source: Journal of Immunoassay and Immunochemistry - June 20, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunohistochemical expression HIF1 α in chronic plaque psoriasis, an association with angiogenesis and proliferation.
Immunohistochemical expression HIF1α in chronic plaque psoriasis, an association with angiogenesis and proliferation. J Immunoassay Immunochem. 2018 May 17;:1-14 Authors: Abdou AG, Farag AGA, Hammam M, Taie DM, Abdelaziz RA Abstract Psoriasis is characterized by excessive cell proliferation, angiogenesis, and regions of hypoxia. Hypoxia stimulates production of hypoxia inducible factors (HIFs) such as HIF1α. The aim of the present study is to investigate the possible role of HIF1α in pathogenesis of psoriasis and to correlate its expression with angiogenesis and proliferation in invol...
Source: Journal of Immunoassay and Immunochemistry - May 19, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of performance testing of different rapid diagnostic kits in comparison with EIAs to validate detection of hepatitis B virus among high risk group in Nigeria.
Authors: Afolabi AY, Bakarey AS, Adewumi MO Abstract BACKGROUND: Hepatitis B virus (HBV) causes chronic liver-associated diseases and its early detection is of high public health importance. Its diagnosis is mainly based on immunological assays among which Enzyme-Linked Immunosorbent Assay (ELISA) and rapid tests are the most common and widespread methods. However, a major challenge is the discordance of results of any two laboratory assays which cannot be easily resolved. Therefore, this study was designed to evaluate the validity and reliability of commercially available five rapid test kits in comparison with tw...
Source: Journal of Immunoassay and Immunochemistry - May 18, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Hepatitis B virus infection among asymptomatic residents of low income community in Ibadan, Southwest, Nigeria.
CONCLUSION: This study reported relatively high prevalence for HBV infection among asymptomatic population, which is of public health importance and this calls for urgent attention. Therefore, public sensitization on HBV transmission and control for all through voluntary counseling and testing is advocated. PMID: 29757703 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - May 16, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Complete blood count alterations due to the opioid use: what about the lymphocyte-related ratios, especially in monocyte to lymphocyte ratio and platelet to lymphocyte ratio?
This study was designed to compare the complete blood count values of opioid users (N = 61) and healthy subjects (N = 61), particularly monocyte-to-lymphocyte ratio (MLR) and platelet-to-lymphocyte ratio (PLR). PLR, MLR, and percentage of monocyte (MONO%) were significantly lower in opioid use disorder (OUD) group (P = 0.012, P = 0.005, P = 0.000). The area under the ROC curve of MLR and PLR levels for OUD was 0.349 and 0.368. MONO% correlated with substance use duration. Measurements like lymphocyte-related ratios and MONO% in opioid use can be important in substance monit...
Source: Journal of Immunoassay and Immunochemistry - May 16, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

How to assess the best immunohistochemical panel in the diagnosis of malignant pleural mesothelioma in a pathology lab.
CONCLUSION: these results yielded to a diagnostic flowchart that we can use in routine practice and that is in accordance with the literature findings. Many diagnostic and technical pitfalls have to be known by pathologists when dealing with MPM. PMID: 29757709 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - May 16, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of a novel explant culture method for the isolation of mesenchymal stem cells from human breast tumor.
Authors: Sineh Sepehr K, Razavi A, Saeidi M, Mossahebi-Mohammadi M, Abdollahpour-Alitappeh M, Hashemi SM Abstract BACKGROUND: Mesenchymal stem cells (MSCs) were isolated from various sources, including various types of tumors. However choosing an appropriate isolation method is an important step in obtaining cells with optimal quality and yield in companion with economical considerations. The purpose of this study was to isolate of more pure MSCs from human breast tumor tissue by a modified explant culture method. METHODS AND MATERIALS: The tumor tissues (n = 8) was cut into 1 to 3-mm cube-like pieces...
Source: Journal of Immunoassay and Immunochemistry - May 11, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Comparison of the performance of enzyme immunoassays for hepatitis B and C detection in dried blood spot.
This study aims to evaluate two enzyme immunoassays (EIAs) for HBsAg and anti-HCV detection using DBS. Serum was tested using commercial EIA. DBS was tested using optimized EIA developed for serum and commercial EIA developed for DBS (Imunoscreen). Concordances between DBS and serum samples for both markers and EIAs were higher than 97%. Both EIAs demonstrated good performance for HBsAg and anti-HCV detection using DBS, and these methods could be used unchangeably increasing the access for HBV and HCV diagnosis. PMID: 29733758 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - May 9, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Occurrence of Infectious laryngotracheitis outbreaks in commercial layer hens detected by ELISA.
In conclusion, the results of this study indicated a high prevalence of ILT infection in the commercial layer flocks in Konya region, Turkey. In outbreaks, ILT significantly increased the mortality rate and decreased the average live weight in layer hens. PMID: 29424631 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - February 11, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroprevalence of Hepatitis B surface antigenaemia and viral infectivity among liver cancer patients accessing care at a tertiary health facility in Southwest Nigeria.
Authors: Bakarey AS, Jabaru O, Olayiwola O PMID: 29424653 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - February 11, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The inositol-1,2-cyclic phosphate moiety of the cross-reacting determinant, carbohydrate chains, and proteinaceous components are all responsible for the cross-reactivity of trypanosome variant surface glycoproteins.
Authors: Escalona JL, Uzcanga GL, Carrasquel LM, Bubis J Abstract Salivarian trypanosomes evade the host immune system by continually swapping their protective variant surface glycoprotein (VSG) coat. Given that VSGs from various trypanosome stocks exhibited cross-reactivity (Camargo et al., Vet. Parasitol. 207, 17-33, 2015), we analyzed here which components are the antigenic determinants for this cross-reaction. Soluble forms of VSGs were purified from four Venezuelan animal trypanosome isolates, TeAp-N/D1, TeAp-ElFrio01, TeAp-Mantecal01, and TeGu-Terecay323. By using the VSG soluble form from TeAp-N/D1, we found...
Source: Journal of Immunoassay and Immunochemistry - January 25, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Current and emerging EGFR therapies for glioblastoma.
Authors: Artene SA, Tuţă C, Dragoi A, Alexandru O, Stefana Oana P, Tache DE, Dănciulescu MM, Boldeanu MV, Siloşi CA, Dricu A Abstract Glioblastomas (GBM) are the most lethal and hard to treat malignancies in clinical practice. The standard of care for treating GBM involving surgery and adjuvant radiotherapy and concomitant temozolomide (TMZ) has remained virtually unchanged in the past decade. Molecular targeted therapies against cancer-specific structures have reported mediocre results in the treatment of GBM, due to multiple factors such as the presence of the blood brain barrier (BBB) or a vast array of mole...
Source: Journal of Immunoassay and Immunochemistry - January 9, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Immunological responses against vancomycin-resistant enterococcus faecium and enterococcus faecalis by mice.
Authors: Cho YS, Lee MH, Kim JM, Lee HS, Yoo HS, Park YH, Ryu PD Abstract Methicillin-resistant Staphylococcus aureus (MRSA) infections in humans can currently only be treated with vancomycin. Consequently, vancomycin-resistant Enterococcus spp. pose a serious public health hazard because MRSA can acquire their vancomycin resistance. While the microbiological and genetic characteristics of vancomycin-resistant enterococci (VRE) have been extensively studied, serological diagnostic tools for these organisms are lacking. The VanA and VanB classes of VRE show marked resistance. Here, we identified the VanA and VanB pr...
Source: Journal of Immunoassay and Immunochemistry - January 9, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

About molecular profile of lung cancer in Tunisian patients.
CONCLUSION: This study made us wonder about the possibility of implementing molecular techniques in low income countries and about the necessity of optimizing the financial ressources. PMID: 29308976 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - January 9, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of bead based multiplexed immunoassay for evaluation of midkine, syndecan-1, and ANGPTL4 in patient serum.
Authors: Tarhoni I, Fhied CL, Pool M, Liptay MJ, Bonomi P, Seder CW, Borgia JA Abstract BACKGROUND: Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically-important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients. METHODS: Antigen "capture" antibodies for midkine, syndecan-1, and ANGPTL4 were independently conjugated to MagPlex® Microspheres using standard carbodiimide/NHS-based chemistry. These reagents served as the basis for quantitative sandwich as...
Source: Journal of Immunoassay and Immunochemistry - January 9, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Kinetics study on recombinant alkaline phosphatase and correlation with the fluorescent signal generated.
Authors: Susini V, Rossi VL, Sanesi A, Drazek L Abstract Alkaline phosphatase (AP) (EC 3.1.3.1) is one of the most used enzymes in immunoassays. In VIDAS® assays (bioMérieux, Marcy l'Etoile, France) AP catalyses the hydrolysis of 4-Methylumbelliferyl phosphate (4-MUP) in 4-Methylumbelliferone (4-MU) producing a fluorescent signal. This work introduces an original method of characterization of the kinetics parameter Km, Vmax and Kcat of AP embedded in VIDAS® assays. Assessment of such constants allows us to predict the fluorescent signal generated for given amounts of enzyme and its associated substra...
Source: Journal of Immunoassay and Immunochemistry - December 16, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

EOV "Editorial Board".
EOV "Editorial Board". J Immunoassay Immunochem. 2017;38(6):ebi Authors: PMID: 29192881 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - December 3, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seeding of the Mucosal Leukocytes in the HALT and Trachea of White Leghorn Chickens.
Authors: Krunkosky M, García M, Beltran Garza LG, Karpuzoglu-Belgin E, Levin J, Williams RJ, Gogal RM Abstract Characterizing the immune cells of the head associated lymphoid tissues (HALT) and trachea during maturation in young birds is critical to understanding the immunological responses to avian respiratory diseases and vaccines. Selected mucosal leukocytes of the conjunctiva-associated lymphoid tissue (CALT), Harderian gland (HG), nasal associated lymphoid tissue (NALT) and trachea from 4, 6, 8 and 10-week-old chickens were enumerated and phenotyped. HG, NALT, and trachea cellularity increased as the bi...
Source: Journal of Immunoassay and Immunochemistry - November 18, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Extraction, purification of Prostate Specific Antigen (PSA) and establishment of radioimmunoassay system as a diagnostic tool for prostate disorders.
This study aimed to provide an easy and effective method for extraction and purification of prostate specific antigen (PSA) from human seminal fluid with high quantity (14 mg) and high purity (98%). The obtained PSA was injected into rabbits for production of anti-PSA polyclonal antibody (titer 1/1000), labeled with radioactive iodine-125 for preparation of radioactive PSA tracer (purity 98 ± 1.8% and specific activity 64 ± 1.9 µCi/µg), and used in preparation of PSA standards. All prepared components can be used in PSA immunoassays specially radioimmunoassay (RIA) ki...
Source: Journal of Immunoassay and Immunochemistry - November 18, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of UMELISA( ®) T4 Neonatal and UMELISA(®) T4 using polystyrene plates coated with anti-thyroxine (T4) monoclonal antibodies.
Evaluation of UMELISA(®) T4 Neonatal and UMELISA(®) T4 using polystyrene plates coated with anti-thyroxine (T4) monoclonal antibodies. J Immunoassay Immunochem. 2017 Nov 16;: Authors: Castells EM, González EC, Pérez PL, Del Río L, Tejeda Y, Perea Y, Martín O, Espinosa M, Rivero JA, Frómeta A Abstract Congenital hypothyroidism is one of the most common preventable causes of mental retardation. The Center of Immunoassay has developed the UMELISA(®) T4 NEONATAL and UMELISA(®) T4 to determine neonatal T4 levels in dried blood and serum samples. Both reagen...
Source: Journal of Immunoassay and Immunochemistry - November 18, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of novel fluorescent particles applicable for phagocytosis assays with human macrophages.
The objective of this work was the preparation of fluorescent microspheres by a simple method and the evaluation of its applicability in phagocytosis assays by using different human derived cells, differentiated THP-1 cell line and blood monocytes, with flow cytometry measurements for functionality assays. Our results show that microparticles are efficiently internalized in a non-opsonized form and in dose dependent manner by both cellular types. Concerning mechanism we determined that tTG -β3 integrin signaling could be involved in the uptake of these particles. PMID: 29144198 [PubMed - as supplied by publisher] ...
Source: Journal of Immunoassay and Immunochemistry - November 18, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

C-Jun expression in lichen planus, psoriasis and cutaneous squamous cell carcinoma, an immunohistochemical study.
CONCLUSIONS: The marked reduction of C-Jun in SCC in comparison to normal skin and inflammatory skin dermatoses may refer to its tumor suppressor activity. C-Jun expression in SCC carries favorable prognosis. Absence of significant association between C-Jun and Ki-67 either in SCC or inflammatory skin diseases indicates that it does not affect proliferative capacity of cells. PMID: 29144206 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - November 18, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Patterns of serologic markers of hepatitis B virus infection and the risk of transmission among pregnant women in southwestern Nigeria.
This study aimed to evaluate the prevalence of serological HBV markers among pregnant women in Ibadan southwestern Nigeria and to determine the implications for perinatal HBV transmission. This study revealed the presence of varied HBV serological patterns of infection or immunity among pregnant women in Ibadan, Nigeria, and thus the risk of mother to child transmission. PMID: 29035130 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - October 17, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Isolation, Production and Characterization of a New Single Chain Anti-Idiotypic Antibody Against Benzo[A]Pyrene.
Authors: Arnst KV, Studennikov AE, Ustinov VA, Glushkov AN Abstract Polycyclic aromatic hydrocarbons are chemical carcinogens which could induce the development of human cancers. Anti-idiotypic antibodies against benzo[a]pyrene are perspective for human cancer immunoprophylaxis and tumor immunodiagnostic. The purpose of this study was isolating of anti-idiotypic antibodies against benzo[a]pyrene from human lymphocytes naïve phage library. The anti-idiotypic antibody, named B5, was selected. Analysis of the nucleotide and amino acid sequences B5 showed no similarity to known protein databases antibodies. B5 bou...
Source: Journal of Immunoassay and Immunochemistry - October 17, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of serum levels of C3 and C4 complement factors in patients with beta Thalassemia major in Khuzestan Province, Southwest Iran.
This study has shown a significant reduction in serum levels of C3 and C4 in patients compared to controls (P-value
Source: Journal of Immunoassay and Immunochemistry - October 17, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of highly sensitive cell based AKT Kinase ELISA for monitoring PI3K beta activity and compound efficacy.
Authors: Yanamandra M, Kole L, Giri A, Mitra S Abstract Phosphatidylinositol-3 kinase pathway regulates multiple cellular function involving cell survival, growth, motility proliferation, apoptosis, and adhesion. These are deregulated in various diseases like cancer, atherosclerosis, and inflammation. PI3Ks phosphorylate phosphatidylinositol 4,5 biphosphate (PIP2) yielding phosphatidylinositol 3, 4, 5 triphosphate (PIP3) that in turn activates AKT kinase (serine/threonine kinase) which is the central enzyme in regulation of metabolic functions. Due to their implications in disease pathophysiology, PI3K/AKT inhibito...
Source: Journal of Immunoassay and Immunochemistry - October 17, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The Book Corner.
Authors: PMID: 29022828 [PubMed] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - October 16, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Effect of Probiotic strain Lactobacillus acidophilus (LBKV-3) on fecal residual lactase activity in undernourished children below 10 year.
Authors: Hajare ST, Bekele G Abstract Clinically proven Lactobacillus acidophilus strain LBKV-3 intended as probiotc for human was used to test its effect on fecal residual lactase activity in undernourished children below 10 years. The children were selected from malnutrition declared area of Maharashtra (India). One of the major causes of malnutrition is lactose intolerance which leads to diarrhea. The basic consideration in selecting the probiotic strain of Lactobacillus acidophilus-LBKV3 in this investigation was the fact that the organism is isolated from human vaginal surface swab and it was found extens...
Source: Journal of Immunoassay and Immunochemistry - August 31, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroprevalence of Hepatitis B and Delta viruses among HIV infected population attending Antiretroviral clinic in selected health facilities in Abuja Nigeria.
Authors: Ifeorah IM, Bakarey AS, Adeniji JA, Onyemelukwe FN Abstract INTRODUCTION: Tri- infection with human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis D virus (HDV) is rare. There is limited data on the seroprevalence of HIV/HBV/HDV tri-infection especially in Nigeria. The aim of this study was to determine the seroprevalences of HBsAg and HDV among HIV infected individuals attending Anti-retroviral (ARV) clinics in Abuja, Nigeria. METHODS: In this cohort study, blood samples were collected from 1102 (Male = 450; Female = 652) age ranged
Source: Journal of Immunoassay and Immunochemistry - August 31, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Cag Pathogenicity Island-Dependent Up-Regulation Of Matrix Metalloproteinase-7 In Infected Patients With Helicobacter Pylori.
Authors: Sadeghiani M, Bagheri N, Shahi H, Reiisi S, Rahimian G, Rashidi R, Mahsa M, Shafigh M, Salimi E, Rafieian-Kopaei M, Hashemzadeh-Chaleshtori M, Shirzad H Abstract Helicobacter pylori (H. pylori) infection has been involved in the pathogenesis of most important gastroduodenal diseases. Matrix metalloproteinases (MMPs) are a family of secreted, zinc-dependent enzymes collectively capable of degradation of extracellular matrix (ECM) and key player in various inflammatory diseases. Therefore, we examined MMP-7 mRNA levels in the gastric mucosa of patients with H. pylori infection and evaluated the effects of v...
Source: Journal of Immunoassay and Immunochemistry - July 14, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Validated low-volume immunoassay for the reliable determination of direct renin especially valuable for pediatric investigations.
CONCLUSION: The low-volume immunoassay facilitates the reliable collection of pharmacodynamic data in children. PMID: 28699839 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - July 14, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Dynamics of cd4 cell count among hiv infected individuals in lagos, nigeria.
DYNAMICS OF CD4 CELL COUNT AMONG HIV INFECTED INDIVIDUALS IN LAGOS, NIGERIA. J Immunoassay Immunochem. 2017 Jul 12;: Authors: Motayo BO, Olusola BA, Faneye AO, Aturaka S, Oluwasemowo O, Ogiogwa JI Abstract Human Immunodeficiency virus (HIV) infection leads to progressive loss of CD4 T- cells. Antiretroviral therapy has been able to inhibit this process, resulting in significant level of immune recovery and function. Our aim is to investigate the dynamics of CD4 recovery among HIV patients in Lagos, Nigeria. 213 HIV positive individuals were enrolled between October 2007 and May 2008, and followed up fo...
Source: Journal of Immunoassay and Immunochemistry - July 14, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of a novel point-of-care test for the rapid semi-quantitative detection of allergen-specific immunoglobulin E.
CONCLUSIONS: FastCheckPOC 20 is reliable to determine allergen-specific IgE in human whole blood and plasma/serum samples. PMID: 28692338 [PubMed - as supplied by publisher] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - July 12, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The Role of Human Urotensin-II in Patients with Hypertrophic Cardiomyopathy.
This study included 40 HCM patients (60% males and 40% females) and were compared to 30 healthy control subjects (47% males and 53% females. All patients underwent extensive clinical, laboratory, and echocardiographic. Blood samples were taken to test for serum hUT-II levels by commercial ELISA Kit. RESULTS: Serum hUT-II was significantly higher (p
Source: Journal of Immunoassay and Immunochemistry - July 9, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

First Report of Immunohistochemical Detection of Peste Des Petit Ruminants, Parainfluenza 3 and Respiratory Syncytial Viral Antigens in Lungs of Nigerian Goats.
This study determined the of involvement of PPR, PI3 and RS viruses in the pathology of caprine pneumonia across Nigeria. 150 goats were selected randomly. PI3 and RSV monoclonal antibodies and PPR polyclonal antibody were used for the immunolocalisation of the antigens. Histologically, 61 of the goats had broncho-interstitial pneumonia, 25 had interstitial pneumonia, 42 had bronchopneumonia, 12 had bronchiolitis and 10 were normal. PPR, PI3 and RS viral antigens were demonstrated in: intact and desquamated bronchial, bronchiolar epithelial cells, macrophages, leukocytes, pneumocytes and giant cells. 23% of the caprine lun...
Source: Journal of Immunoassay and Immunochemistry - July 6, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of a tree shrew-specific interferon-gamma assay.
Authors: Zhang X, Xu J, Wu Z, Zhu W, Dong S Abstract Tree shrews (Tupaia belangeri) are small squirrel-like mammals closely related to primates. Due to their susceptibility to several human viruses, tree shrews have been proposed as potential animal models for the study of human viral infections. However, there are no standardized assays currently available for the detection of tree shrew-specific interferon (IFN)-γ, a major cytokine secreted during the antiviral immune response. Herein, we developed a novel enzyme-linked immunosorbent assay (ELISA) for the quantification of IFN-γ in tree shrew serum sa...
Source: Journal of Immunoassay and Immunochemistry - July 6, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research