Class switch recombination process in ataxia telangiectasia patients with elevated serum levels of IgM.
This study was designed to investigate class switch recombination (CSR) as a critical mechanism in B lymphocytes' maturation to produce different isotypes of antibody in response to antigen stimulation in AT cases with HIgM presentation. Quantitative IgE production after stimulation by IL-4 and CD40L was considered as an indicator for CSR function. We also compared their results with sex and age matched AT patients without HIgM presentation. We report four AT patients with recurrent infections during infancy and high serum levels of IgM. Laboratory evaluations revealed defective CSR while none of the three AT patients with...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Express immunochromatographic detection of antibodies against Brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level.
Authors: Sotnikov DV, Byzova NA, Zherdev AV, Eskendirova SZ, Baltin KK, Mukanov KK, Ramankulov EM, Sadykhov EG, Dzantiev BB Abstract An immunochromatographic test system was developed for rapid detection of the levels of specific IgG antibodies to Brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. The pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. The results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme ass...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Effects of red blood cell lysing solutions on the detection of peripheral basophils of healthy normals and SLE patients by flow cytometry.
Authors: Pan Q, Ye L, Deng Z, Li L, Liu H Abstract To evaluate the effect of four widely used red blood cell lysing solutions on counting and measurement of activation marker of peripheral basophils in normals and systemic lupus erythematosus (SLE) patients by flow cytometry. Our results showed that the light scatter properties including FS and SS value of leukocytes in whole blood were preserved when whole blood samples were lysed in RBC Lysis Buffer and FACS Lysing Solution, while were affected when lysed in distilled water or ACK. By counting basophils, RBC Lysis Buffer and FACS Lysing Solution were almost the s...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Antiviral activity of virocidal peptide derived from NS5A against two different HCV genotypes: an in vitro study.
In conclusion, the C5A virocidal peptide has potent antiviral activity against HCV. PMID: 24606010 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Binding sites of mosquitocidal toxins of Pseudomonas fluorescens and Bacillus subtilis on pupae and larvae of Culex quinquefasciatus.
Authors: Mary KA, Paily KP, Hoti SL, Balaraman K Abstract Two of the potential bacterial isolates, viz., Pseudomonas fluorescens (VCRC B-426) and Bacillus subtilis (VCRC B-471) whose toxins kill the mosquito pupae/larvae have been identified at our center. As the mode of action of these bacteria are not known, an attempt was made to find out the binding sites of the toxic proteins through immunological methods. Antibodies were raised in BALB/c mice and egg yolk system of chicken layers against the mosquitocidal proteins. The antibodies showed specific binding on to the cephalic and thoracic cuticle of the pupae as ...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Expression of topoisomerase II alpha, ki67, and p53 in primary non-muscle-invasive urothelial bladder carcinoma.
Authors: Ben Abdelkrim S, Rammeh S, Ziadi S, Tlili T, Jaidane M, Mokni M Abstract To assess the prognostic value and clinicopathological correlate of the expression of topoisomerase II alpha, ki67, and p53 in non muscle-invasive urothelial bladder carcinoma. Seventy one cases of formalin-fixed, paraffin-embedded bladder biopsy specimens diagnosed as non muscle invasive urothelial carcinoma were processed by searching our surgical pathology files from 2001-2003. The patients were followed-up for 3-77 months (median = 28). In each case, one tissue block was chosen for immunohistochemical expression of ki67, topoisome...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The usage of phage mini-antibodies as a means of detecting ferritin concentration in animal blood serum.
Authors: Staroverov SA, Volkov AA, Fomin AS, Laskavuy VN, Mezhennyy PV, Kozlov SV, Larionov SV, Fedorov MV, Dykman LA, Guliy OI Abstract Mini-antibodies that have specific ferritin response have been produced for the first time using sheep's phage libraries (Griffin.1, Medical Research Council, Cambridge, UK). Produced phage antibodies were used for the first time for the development of diagnostic test kits for ferritin detection in the blood of cattle. The immunodot assay with secondary biospecific labeling is suggested as means of ferritin detection in cow blood serum (antiferritin phage antibodies and rabbit ant...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A new method to fabricate an electrochemical aptasensor to assay adenosine deaminase concentration using an assistance DNA.
Authors: Huang Q, Wang X, Zheng L Abstract A novel strategy for the fabrication of electrochemical aptasensor is proposed in this work. This strategy has been employed to develop an aptasensor for the detection of adenosine deaminase concentration. A probe which contains adenosine aptamer and modified with ferrocene is adopted in our strategy as the core element. Moreover, an assistance DNA which can hybridize with the probe was also been employed in our strategy. The enzymatic reaction of adenosine catalyzed by adenosine deaminase plays a key role as well in the regulation of the hybridized complex. The formation ...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroprevalence rates of hepatitis C virus (HCV) antibody and hepatitis B virus surface antigen (HBsAG) in blood donors in a southwestern nigerian city.
We report the prevalence rates of anti-HCV Ab and HBsAg in blood donors from Ogun State, Nigeria. Our results reveals higher rate of HBsAg and evidence of co-infection with both viruses, illiteracy was the only variable associated with HBV infection. We advocate for the inclusion of anti-HBc or HBeAg in donor screening in our environment. PMID: 24628383 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Design of immunoradiometric assay for gonadotropins using a common detection antibody: an experience.
DESIGN OF IMMUNORADIOMETRIC ASSAY FOR GONADOTROPINS USING A COMMON DETECTION ANTIBODY: AN EXPERIENCE. J Immunoassay Immunochem. 2014 Mar 21; Authors: Vrinda PC, Paradkar SN, Samuel G, Sivaprasad N Abstract We share our experience in the development of immunoradiometric assay (IRMA) for gonadotropins, FSH and LH using common tracer concept. Commercially procured, monoclonal antibody against 'common epitope' of FSH and LH was radiolabeled and epitope specific monoclonal antibody against β-subunit of FSH and LH was chemically immobilized and used as a capture system. In an inclusive assay procedure d...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Detection of anti-cyclic citrullinated peptide using a time-resolved fluoroimmunoassay.
Authors: Xiao J, Hu Z, Liu J, Li M, Zou Y Abstract In an effort to enhance the linear range of anti-CCP we developed a new immunoassay based on time-resolved fluoroimmunoassay. The precision, sensitivity, specificity, and stability of the assay were evaluated ELISA set as control. The anti-CCP IgG TRFIA kit we established had a wider detectable range than commercial ELISA ones. With regard to intra- and inter-assay precision, the TRFIA kit was better than threee commercial ELISA ones. The mean recovery rate was 101.0%. The TRFIA we developed for anti-CCP IgG detection yielded a more sensitive and reliable method fo...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A new electrochemical aptasensor for the analysis of the vascular endothelial growth factor.
Authors: Lv Z, Wang K, Zhang X Abstract The vascular endothelial growth factor (VEGF) is a mitogen and important regulator of angiogenesis by activation the VEGF-receptor tyrosine kinases in endothelial cells. VEGF finds growing interest as a therapeutic target, and its analytical detection has important implications as biomarker for different clinical disorders. Herein, we propose a new strategy to fabricate elegant electrochemical aptasensors, and this strategy has been employed to develop a biosensor for the assay of the concentration of VEGF. In our strategy, an electroactive probe, hemin, is not covalently mod...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of an indirect ELISA for detection of E. Coli antibodies in cow serum using a recombinant OmpT as antigen.
Authors: Tong C, Wu Z, Yu L, Fan Z, Chen L, Hu R, Ma J, Song B, Zhu Z, Cui Y Abstract The aim of this article was to develop an indirect enzyme-linked immunosorbent assay (ELISA) for efficient detection of the infection of E. coli in cattle. OmpT, a highly conserved protease in all E. coli strains, was successfully expressed in E. coli XL-1-Blue strain with PET32a vector. Molecular weight of recombinant protein was identified by analyzing SDS-PAGE and the immunogenicity of OmpT was confirmed by Western Blotting. The recombinant OmpT was then employed as capture antigen in the ELISA. The antigen concentration and se...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Over-expression of EGFR is closely correlated to poor prognosis in Tunisian patients with non-small cell lung adenocarcinoma.
Authors: Arfaoui A, Kriaa L, Znaidi N, Gritli S, Bouacha H, Zermani R, Rammeh S Abstract We studied epidermal growth factor receptor (EGFR) expression profile with the aim of an individualized therapy for patients with non-small cell lung cancer (NSCLC) from whom tumor materials are not sufficient for molecular investigations. Using immunohistochemistry, we found a markedly increased EGFR expression with significant difference in term of intensity and distribution from normal mucosa to primary tumors (p
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Purification and molecular characterization of IgM in olive barb, Puntius Sarana.
Authors: Das A, Sahoo PK, Mohanty J, Garnayak SK Abstract In the present article, immunoglobulin (Ig) of Puntius sarana (a vulnerable medium carp species) was purified by affinity chromatography, characterized, and identified as only IgM type with a native molecular weight of 879 kDa having one heavy (88 kDa) and one light (26 kDa) chain. Further, the developed rabbit antisera against IgM was found to be quite specific to P. sarana IgM and used in ELISA to measure the antibody titer in P. sarana at different time periods, against an antigen (hemocyanin) injection with and without adjuvant. The antibody titer was si...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

In vitro assessment of the biologic activity of interferon beta formulations used for the treatment of relapsing multiple sclerosis.
Authors: Scagnolari C, Selvaggi C, Di Biase E, Fraulo M, Dangond F, Antonelli G Abstract A new formulation (NF) of subcutaneous (sc) interferon (IFN) β-1a was developed in an attempt to improve injection tolerability and immunogenicity. We compared antiviral and IFNβ-stimulated gene (ISG) activities of IFNβ-1a sc NF with IFNβ-1a sc original formulation and IFNβ-1b sc. When equivalent unit amounts were compared, the IFNβ formulations demonstrated similar antiviral activity and induced similar levels of ISG mRNA. However, on a weight basis (ng/mL), significantly more IFNβ-1b sc was ...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of enzyme-linked immunosorbent assay for estimation of urinary albumin.
Authors: Shrivastav TG, Kariya KP, Prasad PK, Chaube SK, Kumar D Abstract Yearly estimation of urinary albumin is a prerequisite for predicting renal status in Diabetes Type II patients with negative dipstick results for overt proteinuria. A simple, sensitive, and cost-effective enzyme linked immunosorbent assay (ELISA) for urinary albumin has been developed using human serum albumin antiserum (HSA-antiserum), HSA-biotin, and streptavidin-horseradish peroxidase (SA-HRP) conjugates. To the antibody-coated wells, 100 μL of HSA standards followed by 1:100 diluted urine samples in duplicate were added and then 50 &m...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Mechanisms of immune injury and heterogeneity of bone marrow hematopoietic cells island in patients with auto-immuno-related hematocytopenia.
Authors: Sun L, Wu Q, Han B, Li G, Sun Z, Zhang J, An L Abstract Because of environmental pollution more and more people are suffered with auto-immuno-related hematocytopenia (AIRH). Serum IL-12, IL-17, and IFN-γ levels were detected by ELISA and lymphocyte subsets were analyzed by flow cytometry. Peroxidase (POX) and HLA-DR of immune cells were detected by cytochemical and immunochemical staining. Cells expressing anti-human IgG, FcγR II, MR, and other molecules in HI were detected by immunofluorescence. Serum IL-12, IL-17, and IFN-γ levels of patients were significantly higher than control group...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Cytological smears and cell blocks on endobronchial aspirates complement each other and allow a higher diagnostic yield.
Authors: Mlika M, Chebbi C, Haddouchi C, Mezni F Abstract The accurate diagnosis of lung carcinoma has become compulsory especially after the introduction of new target therapies. Most of the patients are non operable. This fact points out the diagnostic importance of the cytology specimen. Our target was to assess the diagnostic efficacy of bronchial smears and cell blocks by comparing their results with those of the final diagnoses made on biopsies. We conducted a prospective study of 55 bronchial aspirates. It showed a sensitivity of 100% and a specificity of 100% when coupling both techniques. These results put...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of the microvessel density and the expression of metalloproteases 2 and 9 and ttf1 in the different subtypes of lung adenocarcinoma in Tunisia: a retrospective study of 46 cases.
Authors: Mlika M, Makhlouf C, Boudaya MS, Haddouchi C, Tritar F, Mezni F Abstract Despite the new available histologic classification of lung cancers, it remains difficult to assess the real prognostic relevance of the different subtypes of lung adenocarcinomas. Facing the prognostic relevance of microvessel density (MVD) and the expression of metalloproteases and thyroid tyrosine factor 1 (TTF1) in different cancers, we tried to compare these parameters in the different subtypes of lung adenocarcinomas. We conducted a retrospective study of 46 adenocarcinomas surgically resected. An immunohistochemical study using...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Resistance-based biosensor of Multi-Walled Carbon Nanotubes.
Authors: Kolosovas-Machuca ES, Vera-Reveles G, Rodríguez-Aranda MC, Ortiz-Dosal LC, Segura-Cardenas E, Gonzalez FJ Abstract Multi-Walled Carbon Nanotubes (MWNTs) are a good choice for resistive biosensors due to their great resistance changes when immunoreactions take place, they are also low-cost, more biocompatible than single-walled carbon nanotubes, and resistive measurement equipment is usually not expensive and readily available. In this work a novel resistive biosensor based on the immobilization of an antigen through a silanization process over the surface of Multi-Walled Carbon Nanotubes (MWNTs) is ...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of ALS assay of TB-SA for diagnosis of pulmonary tuberculosis.
Authors: Jiao J, Wang MS, Yang XG, Wang XF Abstract The study evaluated tuberculosis specific antigen (TB-SA) antibody in lymphocyte supernatant (ALS) assay for diagnosis of pulmonary tuberculosis (PTB). The mean concentration of TB-SA ALS in PTB patients was significantly higher than that of two control groups (non-PTB patients and healthy subjects, P
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The immune injury effects and clinical significance of eosinophils in auto-immune-related hematocytopenia.
Authors: Sun LF, Han B, Wu QQ, Wang GC, Hao HF, Du YH, Zhang Y Abstract Eosinophils (EOS) quantity, active state, peroxidase activity (POX), and HLA-DR expression in bone marrow of 176 Auto-Immune-Related Hematocytopenia (AIRH) patients were analyzed. Immunofluorescent staining (IF) is performed to observe the expression of immunizing molecules on EOS. In serum of AIRH patients the levels of IL-4, IL-5, IL-6, IL-12, IL-17, and IFN- γ were increased but there was no significance on IL-2 level. In marrow of AIRH, activated EOS expressed POX, and other molecules, it played various cell-mediated immunity injury r...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Detection of reactive oxygen species during the cell cycle under normal culture conditions using a modified fixed-sample staining method.
This study demonstrates a modified fixed-sample method that can be used to measure intracellular ROS production during the cell cycle using standard immunostaining techniques. PMID: 24749949 [PubMed - indexed for MEDLINE] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A label-free electrochemical aptasensor for the analysis of the potassium ion.
Authors: Ji X, Li J, Yang C Abstract Herein, a simple and novel electrochemical method for the detection of potassium ions (K(+)) was developed. In the presence of potassium ions, the potassium ions aptamer will form a G-quadruplex complex. Thus, further addition of hemin in the presence of potassium ions will lead to the formation of a recombined G-quadruplex. Then the electroactive label, hemin, will give an electrochemical response. The linear range of the method covered a large variation of K(+) concentration from 0.1 nM to 0.1 μ M and the detection limit of 0.1 nM was obtained. Moreover, this assay was able...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

The spatiotemporal expression and localization implicates a potential role for SerpinB11 in the process of mouse spermatogenesis and apoptosis.
In this study, the spatiotemporal expression of SerpinB11 in the mouse testis from postnatal 1-60 d was checked, the SerpinB11 protein strongly localized in the intermediate spermatogonia, B-type spermatogonium, preleptotene spermatocyte, leptonema spermatocyte, zygotene spermatocyte, but weakly localized in the pachytene spermatocyte, diplotene spermatocyte, sphere sperm, and the apoptotic sperm was positive stained of SerpinB11 protein, the localization of cell cycle marker CDK4 and meiosis marker SCP3 were investigated, and the SCP3 and SerpinB11 colocalized in the intermediate spermatogonia, B-type spermatogonium, prel...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroepidemiology and high negativity of IgG antibodies against human papillomavirus (HPV) Type 6, 11, 16, and 18 virus-like particles in women of childbearing age in Port Harcourt, Nigeria.
This study reports the seroepidemiology and high negativity of IgG antibodies against the most common low- and high-risk HPVs among sexually active women of childbearing age in Port Harcourt, Nigeria. A total of 182 consented women (age range 19-45 years) were consecutively recruited to participate in the study. Using a Performa specifically designed for this study, pertinent socio-demographic/behavioral data were collected. Five 5 mL blood samples were also collected (aseptically) from each woman. Plasma of each sample was assayed for HPV-6/11/16/18 virus-like particles using a HPV IgG ELISA kit (Dia.Pro). The study showe...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Relationship between SDF-1G801A polymorphism and its expression in Tunisian patients with colorectal cancer.
Authors: Amara S, Chaar I, Khiari M, Ounissi D, Hmida AB, Mzabi SR, Bouraoui S Abstract The aim was to evaluate the relationship between SDF-1G801A polymorphism and its immunohistochemical expression in colorectal cancer tissues in the Tunisian cohort. The molecular and immunohistochemical analysis showed that SDF-1G801A polymorphic variant was higher in CRC patients with TNM stage II and III, the SDF-1 expression was significantly increased from normal mucosa to primary tumor (p
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Measles vaccine potency and sero-conversion rates among infants receiving measles immunization in Ilorin, Kwara State, Nigeria.
This study was designed to assess the seroconversion rate of measles vaccine among infants receiving measles immunization in Ilorin, Nigeria. The pre- and post-measles vaccination sera of the children were tested using the Haemagglutination Inhibition test. The measles vaccines administered at the immunization centre were also tested for their potency using in-vitro titration method. Only 286 (71.5%) of the vacinees returned to give post-vaccination samples. All the infants screened had low pre-vaccination measles antibody titers. Thirty one (8.0%) of the infants had measles prior to vaccination. The seroconversion pattern...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Polyclonal murine and rabbit antibodies for the bile acid isolithocholic acid.
This study reports the production of antibodies for isolithocholic acid, the isomer of the extensively studied lithocholic acid. The IgG titer and affinity maturation were monitored during the immunizations of three mice and two rabbits. In both animal models, polyclonal antibodies with a high selectivity and affinity were produced. The development of a direct competitive ELISA with a test midpoint of 0.69 ± 0.05 μ g/L and a measurement range from 0.09-15 μg/L is reported. Additionally, the crystal structure of isolithocholic acid is described for the first time. PMID: 24845039 [PubMed - indexed for MEDLIN...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Production and characterization of monoclonal antibodies against horse immunoglobulins useful for the diagnosis of equine diseases.
Authors: Di Febo T, Luciani M, Ciarelli A, Bortone G, Di Pancrazio C, Rodomonti D, Teodori L, Tittarelli M Abstract Monoclonal antibodies (MAbs) against horse IgG were produced by immunizing Balb/c mice with purified horse IgG and were characterized in indirect ELISA versus purified immunoglobulins from donkey, cow, buffalo, sheep, pig, and chicken. Three MAbs (1B10B6C9, 1B10B6C10, 1B10B6E9) reacted only with horse and donkey IgG and IgM and, in western blotting, were specific for the Fc fragment of equine IgG. MAb 1B10B6E9 was used in chemiluminescent immunoblotting assay for the diagnosis of dourine and in indire...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Association of plasma interleukin-18 levels and polymorphisms in interleukin-18 gene with outcomes of hepatitis C virus infections: a meta-analysis.
Authors: Niu ZL, Zhang PA, Tong YQ Abstract Several polymorphisms in the interleukin-18 (IL-18) gene and plasma IL-18 levels have been reported to influence hepatitis C virus (HCV) infection. However, the published findings have been conflicting. We conducted meta-analyses of randomized, controlled trials to address the association of IL-18 polymorphisms and plasma IL-18 levels and the outcomes of HCV infection. The results showed that there was no evidence for an association between the HCV infections and the polymorphisms genotypes of IL-18. However, plasma IL-18 levels were found higher in HCV infection patients...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Evaluation of the analytical and clinical performances of time-resolved fluoroimmunoassay for detecting carcinoma antigen 50.
This study aims to evaluate the analytical and clinical performances of this kit. Precision, accuracy, specificity, sensitivity, stability, and endogenous interference of this kit are evaluated. Reference range is established. Coincidence rate and correlation between TR-FIA and RIA are evaluated. ROC is adopted to evaluate the diagnostic performance. This kit shows excellent precision with a coefficients of variation (CVs) ranged from 2.2-9.3%, accuracy (average recovery, 98.5%), sensitivity (minimum detectable concentration is 0.2 U/mL), specificity (all cross-reactivity is less than 0.1% except CA199, which is 0.175%), a...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development and evaluation of a truncated recombinant NS3 antigen-based indirect ELISA for detection of pestivirus antibodies in sheep and goats.
This study demonstrates the efficacy of truncated recombinant NS3 antigen based ELISA for seroepidemiological study of pestivirus infection in sheep and goats. PMID: 25118572 [PubMed - indexed for MEDLINE] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Women with osteoarthritis have elevated synovial fluid levels of insulin-like growth factor (IGF)-1 and IGF-binding protein-3.
Authors: Hooshmand S, Juma S, Khalil DA, Shamloufard P, Arjmandi BH Abstract The present study explores the possible connection between synovial fluid concentrations of insulin like growth factor (IGF-1), IGF-binding protein (IGFBP-3), leptin, and C-reactive protein (CRP) in osteoarthritis (OA). Synovial fluid specimens were obtained from a total of thirty-four individuals with and without OA. Protein-normalized measurements of IGF-1, IGFBP-3, and leptin concentrations in synovial fluid showed significantly (P
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

CD56 antibody: old-fashioned or still trendy in endocrine lung tumors.
Authors: Mlika M, Zendah I, Braham E, El Mezni F Abstract BACKGROUND: In 2010, the World Health Organization published a new classification of the endocrine tumors based on the mitotic rate and index. Concerning lung endocrine tumors, the classification of 2004 remains acceptable and widely approved. We noticed in many publications that the most used antibodies in these tumors are chromogranin and synaptophysin. This finding let us wonder about the diagnostic utility of the CD56 antibody which is widely used in our department. MATERIAL AND METHODS: Sixty-nine endocrine lung cancers were diagnosed over a 12-mont...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Statement of retraction.
Authors: PMID: 25148200 [PubMed - in process] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Development of a lateral flow immunoassay strip for rapid detection of CagA antigen of Helicobacter pylori.
Authors: Karakus C Abstract About half of the world populations are known to be infected with Helicobacter pylori. The CagA antigen secreting strains provoke severe mucosal damages and act as a risk factor for the development of peptic ulceration and gastric cancer. A lateral flow immunoassay (LFIA) strip was developed based on sandwich format for rapid detection of CagA antigen of H. pylori using gold conjugated monoclonal antibody. This LFIA strip will provide a good aid in the diagnosis of CagA-secreting H. pylori within 10 min instead of time consuming, expensive and laborious invasive approaches. PMID: 25...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Genotyping by randomly amplified polymorphic DNA of bacteriocin producing Lactobacillus acidophilus strains from Nigeria.
This study determined the genotyping of bacteriocin producing Lactobacillus acidophilus strains recovered from Nigerian yogurts. Yogurt samples were collected from four different states of South West regions of Nigeria. Isolates were obtained from MRS Medium and biochemically characterized. This was further confirmed by API50CH. The bacteriocin positivity and activity was determined. Genomic characterization of our Lactobacillus acidophilus strains was done with randomly amplified polymorphic DNA-PCR. All yogurt samples containing Lactobacillus acidophilus strains meet the probiotic requirement of ≥10(6) cfu/mL. The gel...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

HBV and HIV coinfections among intending blood donors in Port Harcourt, Nigeria.
This study estimated the seroprevalence of HBV/HIV coinfections among intending blood donors in Port Harcourt, Nigeria. In this cohort study, we collected blood samples from 178 intending blood donors who were confirmed to be HIV-seropositive at the University of Port Harcourt Teaching Hospital (UPTH) from September 2012 to June 2013. Commercial ELISA was used to assay for the presence of HBsAg among these subjects. The overall prevalence was found to be 6.7%. Sex and education (P 0.05) in this study. We also observed a high overall HBV/HIV co-infection seronegativity of 93.3% among these blood donors. Group-specific seron...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Serum HSV-1 and -2 IgM in pregnant women in Port Harcourt, Nigeria.
Authors: Okonko IO, Cookey TI, Okerentugba PO, Frank-Peterside N Abstract The present study was undertaken for the purpose of finding IgM antibodies against HSV-1 and 2 infections among pregnant women and also to evaluate correlation of Serum HSV-1 and 2 IgM in these pregnant women. A total of 180 pregnant women attending antenatal clinic at Braithwaite Memorial Specialist Hospital (BMSH) in Port Harcourt, Nigeria were consecutively recruited, after they had given consents to participate in the study. Serum of each sample was assayed for HSV-1&2 IgM antibody using a commercial ELISA. Five (2.8%) of the pregnant...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

A novel interaction mode between acrylamide and its specific antibody.
Authors: Zhou S, Wang D, Zhang C, Zhao Y, Zhao M, Wu Y Abstract Since the discovery of high-level acrylamide (Acr) contamination in food, extensive international studies have focused on its toxicity and detection. By using a novel antigen synthetic strategy, we have successfully obtained a specific antibody towards acrylamide (Acr-Ab). Herein, the Acr-Ab and its interactions with Acr were characterized. Enzyme-linked immunosorbent assay (ELISA) and dynamic light scattering (DLS) investigations revealed that the conformational structure of Acr-Ab was sensitive to buffers. It showed a satisfied immunoreactivity in ph...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Electro-optical study of the exposure of Azospirillum brasilense carbohydrate epitopes.
Authors: Guliy OI, Matora LY, Dykman LA, Staroverov SA, Burygin GL, Bunin VD, Burov AM, Ignatov OV Abstract The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions. To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7. After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 2...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Preparation, identfication, and activity assay of lamprey (lampetra japonica) natural intelectins.
Authors: Su P, Zheng Z, Pang Y, Xue Z, Gou M, Han Y, Liu G, Zan Q, Li Q Abstract Intelectins play an important role in innate immune response. In a previous study, lamprey inteletins purified by galactose-Sepharose were inactive and insoluble. Herein, we provided a simple and effective method to purify natural intelectins from the serum of lamprey (Lethenteron japonicum) using proteinG agarose. SDS-PAGE, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and mass spectrometry (MS) were used to analyze the purified proteins. The purified proteins were identified to be lamprey serum lectin and intelectinB....
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Extensive study of cytokine, chemokines expression in peripheral blood mononuclear cells upon CpG stimulation.
Authors: Peng Y, Zhang L Abstract The innate and adaptive immune response could be initiated by toll-like receptors (TLRs) by recognizing the conserved components of microbes. Among human TLR family, TLR9 was critical in sensing DNA viruses and endogenous DNA. Previous researches confirmed that activation of TLR9 could initiate many important cytokines such as IL-6, IL-8, IL-10, and IFN-β. The aim of this article is to analyze expression of more molecules upon TLR9 agonist stimulation, including tumor-related factors, kinase signal molecules, adhesion molecules, and co-stimulators. Peripheral blood mononuclear...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Nature of immobilization surface affects antibody specificity to placental alkaline phosphatase.
Authors: Kumar M, Khan I, Sinha S Abstract Retention of native conformation of immobilized protein is essential for various applications including selection and detection of specific recombinant antibodies (scFvs). Placental alkaline phosphatase (PAP), an onco-fetal antigen expressed on the surface of several tumors, was immobilized on supermagnetic particles for selection of recombinant antibodies from a human phage display antibody library. The isolated antibodies were found to be cross-reactive to either of the isozymes of alkaline phosphatase, i.e., bone alkaline phosphatase (BAP) or intestinal alkaline phospha...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Seroprevalence of infectious bursal disease virus in local chickens in Udu Local Government Area of Delta State, South East Nigeria.
Authors: Abraham-Oyiguh J, Adewumi MO, Onoja AB, Suleiman I, Sulaiman LK, Ahmed SJ, Jagboro ST Abstract Infectious Bursal Disease Virus (IBDV) poses a great global threat to the poultry industry. Knowledge of the occurrence of the disease is important in the design and implementation of a control program, therefore this study determines the seroprevalence of IBDV in local chickens in Udu Local Government Area of Delta State. 250 chickens were bled by exsanguination and sera obtained were screened using Agar Gel Immunodiffusion (AGID) test. The seropositivity was 51.6%, which is indicates endemicity of the disease. ...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

CD4+, CD8+, CD3+ cell counts and CD4+/CD8+ ratio among patients with mycobacterial diseases (leprosy, tuberculosis), HIV infections, and normal healthy adults: a comparative analysis of studies in different regions of India.
This study is the first of its kind in this region of the country and assumes importance since no other study has reported the values of CD4+ and CD8+ T-lymphocyte counts among patients with mycobacterial diseases (leprosy and TB), HIV infections along with normal healthy individuals of the region, and correlation with clinical presentations of patients. PMID: 25350657 [PubMed - indexed for MEDLINE] (Source: Journal of Immunoassay and Immunochemistry)
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

DNA synthesis in periportal and perivenous hepatocytes of intact and hepatectomized young mice.
Authors: Fernández-Blanco A, Inda AM, Errecalde AL Abstract DNA synthesis of hepatocytes in two areas of Intact and Hepatectomized young mice liver along a circadian period was studied. DNA synthesis was significantly different at all analyzed time points in Intact and Hepatectomized animals. Differences between periportal and perivenous hepatocytes were found in hepatectomized animals at 04/42 and 08/46 hr of day/hour post-hepatectomy. DNAs peak in periportal hepatocytes regenerating liver occurs 4 hr earlier than in perivenous hepatocytes, probably reflecting their shorter G1 phase. Besides, daily mean val...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

Efficacy of generic highly active antiretroviral therapy in HIV-1 infected individuals in Nigeria.
This study appraised these parameters, and efficacy of generic ARVs. Consenting 106 HIV infected ARV naïve patients were enrolled. CD4 T lymphocyte and plasma HIV RNA levels were determined at interval for 24 months. Ninety eight (92.5%) of the patients who completed the follow up in strict adherence to therapy guideline were included in the analysis. Baseline median CD4 T lymphocyte increased from 114 (Range: 6-330) to highest 357 (Range: 15-1036) cells/ μ L at 18 months of therapy, while baseline median plasma viral RNA declined from 4.6 (Range: 2.6-6.0) Log10 copies/mL to undetectable level within three months o...
Source: Journal of Immunoassay and Immunochemistry - November 29, 2015 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research