DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status
The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activities had the highest values in ...
Source: Cryobiology - February 15, 2024 Category: Biology Authors: Nataliia Volkova Mariia Yukhta Anatoliy Goltsev Source Type: research
DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status
The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activities had the highest values in ...
Source: Cryobiology - February 15, 2024 Category: Biology Authors: Nataliia Volkova Mariia Yukhta Anatoliy Goltsev Source Type: research
Impact of caloric restriction on oxidative stress and key glycolytic enzymes in the cerebral cortex, liver and kidney of old and middle-aged mice
In this study, we examined effects of aging and CR on the activities of glycolytic enzymes and parameters of oxidative stress in the cerebral cortex, liver, and kidney of middle-aged (9 months old) and old (18 months old) C57BL6/N mice. Control middle-aged and old mice were fed ad libitum (AL groups), whereas age-matched CR groups were subjected to CR (70% of individual ad libitum food intake) for 6 and 12 months, respectively. There were no significant differences in the activities of key glycolytic and antioxidant enzymes and oxidative stress indices between the cortices of middle-aged and old AL mice. The livers and kid...
Source: Neuropharmacology - February 10, 2024 Category: Drugs & Pharmacology Authors: Myroslava V Vatashchuk Viktoriia V Hurza Nadiia Stefanyshyn Maria M Bayliak Dmytro V Gospodaryov Olga Garaschuk Volodymyr I Lushchak Source Type: research
Black soldier fly larvae meal as a potential modulator of immune, inflammatory, and antioxidant status in gilthead seabream juveniles
In conclusion, up to 45% dietary inclusion of HM showed no adverse effects, improving liver antioxidant status, reducing intestinal oxidative stress, and regulating inflammatory gene expression.PMID:38340781 | DOI:10.1016/j.cbpb.2024.110951 (Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology.)
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - February 10, 2024 Category: Biochemistry Authors: Sara Moutinho Aires Oliva-Teles Filipa Fontinha Nicole Martins Óscar Monroig Helena Peres Source Type: research
Glucose-6-Phosphatase-Dehydrogenase activity as modulative association between Parkinson ’s disease and periodontitis
This study aimed to evaluate these associations in periodontal inflammation. Clinical data and saliva-, serum-, and RNA-biobank samples of 50 well-characterized diametric patients with PK and five age- and sex-matched neurologically healthy participants were analyzed for G6PD function, periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Campylobacter rectus, Fusobacterium nucleatum, and Filifactor alocis), monocyte chemoattractant protein (MCP) 1, and interleukin (IL) 1-beta. Regression analysis was used to identify associ...
Source: Frontiers in cellular and infection microbiology - February 7, 2024 Category: Microbiology Source Type: research
Genetic analysis and molecular basis of G6PD deficiency among malaria patients in Thailand: implications for safe use of 8-aminoquinolines
It was hypothesized that glucose-6-phosphate dehydrogenase (G6PD) deficiency confers a protective effect against malaria infection, however, safety concerns have been raised regarding haemolytic toxicity cause... (Source: Malaria Journal)
Source: Malaria Journal - February 2, 2024 Category: Infectious Diseases Authors: Usa Boonyuen, Beatriz Aira C. Jacob, Jutamas Wongwigkan, Kamonwan Chamchoy, Natsamon Singha-art, Natnicha Pengsuk, Duantida Songdej, Emily R. Adams, Thomas Edwards, Supat Chamnanchanunt, Syazwani Itri Amran, Nurriza Ab Latif, Naveen Eugene Louis and Shami Tags: Research Source Type: research
G6PD and machine learning algorithms as prognostic and diagnostic indicators of liver hepatocellular carcinoma
Liver Hepatocellular carcinoma (LIHC) exhibits a high incidence of liver cancer with escalating mortality rates over time. Despite this, the underlying pathogenic mechanism of LIHC remains poorly understood. (Source: BMC Cancer)
Source: BMC Cancer - January 31, 2024 Category: Cancer & Oncology Authors: Fei Li, Boshen Wang, Hao Li, Lu Kong and Baoli Zhu Tags: Research Source Type: research
Glucose-6-phosphate dehydrogenase deficiency and psychotic disorders: A systematic review
Volume 25, Issue 2, February 2024, Page 151-159 . (Source: World Journal of Biological Psychiatry)
Source: World Journal of Biological Psychiatry - January 31, 2024 Category: Psychiatry Authors: Christopher GandarJames G. ScottNicola Warrena Child and Youth Mental Health Service, Children ’s Health Queensland, Brisbane, Australiab Child Health Research Centre, The University of Queensland, Brisbane, Australiac Queensland Centre for Mental Healt Source Type: research
Late ‐onset aspirin‐related hemolysis and subsequent subdural hemorrhage in patient with glucose‐6‐phosphate dehydrogenase deficiency
Key Clinical MessageAspirin-related hemolysis in G6PD deficiency could be late-onset during long-term administration. Hemolytic anemia could continue for a relatively long time in elder patient with G6PD deficiency, which might be related to other adverse events.AbstractAspirin-related hemolysis in G6PD-deficient individuals was generally reported among patients who received high-dose supplements within several days after ingestion. The safety of long-term and low-dose (50 –325 mg/day) aspirin in patients coexist G6PD deficiency and cardiovascular disease is neglected in clinical practice. In this case, we observed a l...
Source: Clinical Case Reports - January 30, 2024 Category: General Medicine Authors: Jianle Li,
Yicong Chen,
Weisheng Deng,
Jinsheng Zeng Tags: CASE REPORT Source Type: research
Genes, Vol. 15, Pages 186: Comparison of Growth Performance, Carcass Properties, Fatty Acid Profile, and Genes Involved in Fat Metabolism in Nanyang and Landrace Pigs
This study compared the growth, carcass properties, fatty acid profile, lipid-producing enzyme activity, and expression pattern of genes involved in fat metabolism in Nanyang and Landrace pigs. In the study, 32 Nanyang (22.16 ± 0.59 kg) and 32 Landrace barrows (21.37 ± 0.57 kg) were selected and divided into two groups, each with eight pens and four pigs per pen. The trial period lasted 90 days. The findings showed that the Nanyang pigs had lower average daily weight gain and lean percentage and higher average backfat thickness and lipogenic enzyme activities, including for acetyl-CoA carboxyl...
Source: Genes - January 30, 2024 Category: Genetics & Stem Cells Authors: Jinzhou Zhang Shuaitao Meng Heming Wang Chuankuan Zhang Zhe Sun Luyao Huang Zhiguo Miao Tags: Article Source Type: research
Biochanin A - A G6PD inhibitor: In silico and in vitro studies in non-small cell lung cancer cells (A549)
In conclusion, our findings highlight the potential of BA as a therapeutic agent in cancer treatment, specifically by targeting G6PD and related pathways. BA's varied effects, which range from anti-inflammatory capabilities to metastasis reduction, make it an appealing option for future investigation in the development of new cancer therapeutics.PMID:38266663 | DOI:10.1016/j.tiv.2024.105785 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - January 24, 2024 Category: Toxicology Authors: Anjali B Thakkar Ramalingam B Subramanian Sampark S Thakkar Vasudev R Thakkar Parth Thakor Source Type: research
GSE253613 Effect of G6PD loss on gene expression in LKB1 deficient KRAS-driven lung tumor
This study delves into the repercussions of G6PD ablation on the development of lung tumors driven by the KRAS oncogene and deficient in LKB1 (KL). The research involved comparing the growth of KL lung tumors with or without G6PD, revealing a significant inhibition of KL lung tumor growth upon G6PD loss. Subsequently, RNA-seq analysis was employed to identify the alterations in gene expression following G6PD deletion, providing insights into the underlying mechanisms. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - January 23, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Metabolic remodeling of visceral and subcutaneous white adipose tissue during reacclimation of rats after cold
Applied Physiology, Nutrition, and Metabolism, Ahead of Print. Deciphering lipid metabolism in white adipose tissue (WAT) depots during weight gain is important to understand the heterogeneity of WAT and its roles in obesity. Here, we examined the expression of key enzymes of lipid metabolism and changes in the morphology of representative visceral (epididymal) and subcutaneous (inguinal) WAT (eWAT and iWAT, respectively)-in adult male rats acclimated to cold (4 ± 1 °C) for 45 days and reacclimated to room temperature (RT, 22 ± 1 °C) for 1, 3, 7, 12, 21, or 45 days. The relative mass of both depots decreased to a sim...
Source: Applied Physiology, Nutrition, and Metabolism - January 19, 2024 Category: Physiology Authors: Marta Budnar Soskic Tamara Zakic Aleksandra Korac Bato Korac Aleksandra Jankovic Source Type: research
Molecular Characterization of Glucose-6-phosphate Dehydrogenase Deficiency in Oman
CONCLUSIONS: The most common mutation in the Omani population is the Mediterranean mutation (C563T) followed by the variant A- mutation. However, not all participants were found to have a mutation.PMID:38225994 | PMC:PMC10788846 | DOI:10.5001/omj.2023.107 (Source: Oman Medical Journal)
Source: Oman Medical Journal - January 16, 2024 Category: Middle East Health Authors: Ammar Al-Sheryani Hajer Al-Gheithi Muntadhar Al Moosawi Shaoib Al-Zadjali Yasser Wali Murtadha Al-Khabori Source Type: research
G6PD is a prognostic biomarker correlated with immune infiltrates in lung adenocarcinoma and pulmonary arterial hypertension
CONCLUSIONS: This study reveals that G6PD is an immune-related biomarker and a possible therapeutic target for LUAD and PAH patients.PMID:38194707 | DOI:10.18632/aging.205381 (Source: Aging)
Source: Aging - January 9, 2024 Category: Biomedical Science Authors: Rongzhen Ding Shuliu Sang Jian Yi Haiping Xie Feiying Wang Aiguo Dai Source Type: research
