Porcine heart valve, aorta and trachea cryopreservation and thawing using polydimethylsiloxane.
This study is aimed to improve a biological tissues cryopreservation by adding a new heat transfer fluid - polydimethylsiloxane (PDMS). To evaluate its efficiency the porcine biological tissues (heart valves, aortic and trachea fragments) were cryopreserved and thawed in low-viscous PDMS. According to the computer simulation, the midsection cooling rate was up to 490 °C/min and the midsection thawing rate was up to 1140 °C/min with admissible temperature uniformity. Cryoprotectants and liquid nitrogen were not used. The quality of tissue cryopreservation was evaluated using a number of histological and immunohi...
Source: Cryobiology - February 8, 2020 Category: Biology Authors: Lauk-Dubitskiy SE, Pushkarev AV, Korovin IA, Shakurov AV, Burkov IA, Severgina LO, Zherdev AA, Tsiganov DI, Novikov IA Tags: Cryobiology Source Type: research

The presence of synthetic polymers in the maturation medium affects the cryotolerance and developmental capacity after parthenogenic activation of vitrified goat oocytes.
We examined the roles of two polymers, including polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), on in vitro maturation (IVM), embryonic developmental capacity, and cryotolerance of goat oocytes. The present study includes two parts. At first, goat cumulus-oocyte complexes (COCs) were matured in a medium supplemented with 10% fetal bovine serum (FBS), 3 mg/ml PVP, or 1 mg/ml PVA, respectively. Data of oocyte with first polar body, cleavage, and blastocyst following parthenogenetic activation (PA) were recorded. Secondly, after maturation in the above medium, oocytes were vitrified using the Cryotop technique an...
Source: Cryobiology - February 7, 2020 Category: Biology Authors: Wu G, Lv C, Memon S, Liang J, Zhao X, Ouyang Y, Wang S, Jiang Y, Shao Q, Hong Q, Quan G Tags: Cryobiology Source Type: research

Development stage of cryopreserved mussel (Perna canaliculus) larvae influences post-thaw impact on shell formation, organogenesis, neurogenesis, feeding ability and survival.
In this study, effects of cryopreservation on development of trochophore and D-stage larvae of Greenshell™ mussel (Perna canaliculus) were evaluated through histology, light microscopy, scanning electron microscopy, and confocal microscopy. Larvae of both life stages were motile immediately post-thawing, but survival declined rapidly from 4 days post-fertilisation (dpf). At 18 dpf, ∼23% of non-cryopreserved control larvae had progressed to the pediveliger stage, while
Source: Cryobiology - February 7, 2020 Category: Biology Authors: Rusk AB, Alfaro AC, Young T, Watts E, Adams SL Tags: Cryobiology Source Type: research

Dietary omega-3 fatty acids from linseed oil improve quality of post-thaw but not fresh sperm in Holstein bulls.
Abstract Docosahexaenoic acid (DHA), a member of the n-3 fatty acid family present in fish oil, has several positive effects on bovine sperm, including membrane integrity, motility and viability, as well as cold sensitivity. Our objective was to investigate effects of varying amounts of omega-3 fatty acids from linseed oil, administered orally, on quality of fresh and frozen-thawed bull sperm. Twenty fertile Holstein bulls (874 ± 45.38 kg) were randomly and equally assigned to four groups and received encapsulated (rumen-protected) fats for 12 weeks, as follows: group P, 300 g palm oil; ...
Source: Cryobiology - February 6, 2020 Category: Biology Authors: Khoshniat MT, Towhidi A, Reza Yazdi K, Rostami F, Dadashpour Davachi N, Khalooee F, Kastelic J Tags: Cryobiology Source Type: research

Antifreeze protein from Anatolia polita (ApAFP914) improved outcome of vitrified in vitro sheep embryos.
In conclusion, our study suggests that the vitrification is better than the slow freezing method for the conservation of in vitro sheep embryos, and supplementation of ApAFP914 (10 μg/mL) significantly increased the hatching rate of slow-growing embryos after cryopreservation. PMID: 32032586 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - February 4, 2020 Category: Biology Authors: Li X, Wang L, Yin C, Lin J, Wu Y, Chen D, Qiu C, Jia B, Huang J, Jiang X, Yang L, Liu L Tags: Cryobiology Source Type: research

Long-term (24h) cooling of ovarian fragments in the presence of permeable cryoprotectants prior to freezing: Spontaneous pregnancy and baby born after re-transplantation.
Abstract Cancer is the second major cause of death in the world. The problem of post-cancer infertility plays a significant role, because chemotherapy can be gonadotoxic. Cryopreservation of ovarian tissue before cancer therapy with re-implantation after convalescence is the potential key solution to this problem. The aim of this study was to test the viability of cryopreserved human ovarian cortex after long-term cooling in culture medium composed of permeable cryoprotectants. Ovarian fragments from sixteen patients were randomly divided into two groups. After the operation, tissue pieces assigned to both groups ...
Source: Cryobiology - January 31, 2020 Category: Biology Authors: Isachenko V, Morgenstern B, Todorov P, Isachenko E, Mallmann P, Hanstein B, Rahimi G Tags: Cryobiology Source Type: research

Effect of oxygen concentration in anterograde liver persufflation on high energy phosphates and graft function after ischemic preservation.
t A Abstract Here we evaluate the potential of anterograde gaseous oxygen persufflation for graft reconditioning after extended storage times. Pig livers were retrieved and cold-stored in HTK solution for 16 h. Some grafts were subsequently subjected to anterograde gaseous oxygen persufflation via the portal vein for 2 h. Oxygen concentrations for persufflation were either 100% or 40%. The gas was insufflated at a pressure adjusted to 18 mmHg, a pressure required to see gas bubbles leaving at the hepatic vein. Gas flow required for adequate maintenance of persufflation pressure amounted to approx. 300 ml/m...
Source: Cryobiology - January 29, 2020 Category: Biology Authors: Minor T, Lüer B, von Horn C, Paul A, Gallinat A Tags: Cryobiology Source Type: research

Knockout serum replacement is an efficient serum substitute for cryopreservation of human spermatozoa.
In conclusion, the addition of 10% KSR to the sucrose-based freezing solution improves the quality of post-thawed human spermatozoa and may have potential to develop chemically defined freezing medium. PMID: 32004575 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - January 28, 2020 Category: Biology Authors: Taher-Mofrad SMJ, Topraggaleh TR, Ziarati N, Bucak MN, Nouri M, Seifi S, Esmaeili V, Rahimizadeh P, Shahverdi A Tags: Cryobiology Source Type: research

Antimicrobial forcing solution improves recovery of cryopreserved temperate fruit tree dormant buds.
This study examined the effects of an antimicrobial forcing solution (8-hydroxyquinoline citrate and sucrose, 8-HQC) on the cryosurvival and recovery of dormant buds of fruit (Malus x domestica, Prunus armeniaca, Prunus avium, Prunus persica, Pyrus communis), and nut species (Juglans regia, Juglans nigra, Juglans microcarpa). Recovery and shoot development were significantly improved for all the fruit and one nut species (J. microcarpa) treated with the 8-HQC, compared to standard recovery under high humidity alone (P 
Source: Cryobiology - January 28, 2020 Category: Biology Authors: Tanner JD, Minas IS, Chen KY, Jenderek MM, Wallner SJ Tags: Cryobiology Source Type: research

Cryopreservation and transplantation of amputated finger.
In this study, two severed fingers were cryopreserved because the patients' conditions were not allowed to undergo the replantation immediately. The fingers were perfused with the cryopreservation solution through the digital artery at a speed of 1 ml/min, they were frozen following a control rate freezing procedure and stored in the liquid nitrogen. One finger was cryopreserved for 10 days and the other for 30 days. Both fingers were successfully replanted when the patient get better. After 1 year follow-up, the replanted fingers achieved satisfactory appearance and function. Our result demonstrate small composite tissu...
Source: Cryobiology - January 27, 2020 Category: Biology Authors: Wang J, Lin J, Pei Y, Xu Q, Zhu L Tags: Cryobiology Source Type: research

Protective effects of melatonin on male fertility preservation and reproductive system.
Abstract Melatonin is a ubiquitous indoleamine hormone synthesized primarily by the pineal gland. Diverse biological actions of melatonin involve quite complex mechanisms via its membrane receptors. More recently, studies have focused on the role of melatonin in male fertility preservation and male reproductive system. The protective effects of melatonin on immature testicular tissue freshness and activity maintenance and the preservation of sperm and spermatogonial stem cells (SSCs) have attracted considerable attention in recent years. Furthermore, since melatonin has strong antioxidant and anti-apoptotic proper...
Source: Cryobiology - January 27, 2020 Category: Biology Authors: Sun TC, Li HY, Li XY, Yu K, Deng SL, Tian L Tags: Cryobiology Source Type: research

Genome editing of rodents by electroporation of CRISPR/Cas9 into frozen-warmed pronuclear-stage embryos.
In this study, we propose an ethanol-free, slow-freezing method to reduce physical damage to pronuclear-stage embryos followed by the TAKE method. All mouse and rat frozen embryos were survived after electroporation, and 18% and 100% of offspring were edited target gene, respectively. The resulting protocol is an efficient method for producing genome edited animals. PMID: 31987837 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - January 24, 2020 Category: Biology Authors: Kaneko T, Nakagawa Y Tags: Cryobiology Source Type: research

Effect of 'in air' freezing on post-thaw recovery of Callithrix jacchus mesenchymal stromal cells and properties of 3D collagen-hydroxyapatite-scaffolds.
In conclusion, this approach holds promise for the cryopreservation of 'ready-to-use' TECs. PMID: 31972153 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - January 20, 2020 Category: Biology Authors: Mutsenko V, Knaack S, Lauterboeck L, Tarusin D, Sydykov B, Cabiscol R, Ivnev D, Belikan J, Beck A, Dipresa D, Lode A, El Khassawna T, Kampschulte M, Scharf R, Petrenko AY, Korossis S, Wolkers WF, Gelinsky M, Glasmacher B, Gryshkov O Tags: Cryobiology Source Type: research

Comparison of TNC and standard extender on post-thaw quality and in vivo fertility of Thai native chicken sperm.
The objective of the present study was to evaluate a new extender, known as Thai native chicken (TNC) extender compared to BHSV-based and modified Sasaki extenders for freezing chicken semen. Semen from Thai native roosters was collected, pooled, and randomly divided into three groups. Semen was frozen with a simple freezing method using nitrogen vapor and dimethylformamide. In the first experiment, post-thaw motion parameters, viability, acrosome integrity, mitochondrial function, and lipid peroxidation levels were analyzed using computer-assisted sperm analysis, propidium iodide, fluorescein isothiocyanate-conjugate pean...
Source: Cryobiology - January 18, 2020 Category: Biology Authors: Thananurak P, Chuaychu-Noo N, Phasuk Y, Vongpralub T Tags: Cryobiology Source Type: research

Embryo development after vitrification of immature and in vitro-matured equine oocytes.
Abstract Effects of meiotic stage and cumulus status on development of equine oocytes after vitrification was evaluated. Immature oocytes with corona radiata (IMM); in vitro-matured oocytes with corona radiata (MAT CR+); and in vitro-matured oocytes denuded of cumulus (MAT CR-) were vitrified using the Cryotech® method. Warming medium was equilibrated either in 5% CO2 or Air. IMM oocytes underwent in vitro maturation after warming. Recovery, survival, and maturation rates, and cleavage and blastocyst rates after ICSI, were evaluated. Recovery was higher for oocytes warmed in CO2- than Air-equilibrated medium (...
Source: Cryobiology - January 18, 2020 Category: Biology Authors: Angel D, Canesin HS, Brom-de-Luna JG, Morado S, Dalvit G, Gomez D, Posada N, Pascottini OB, Urrego R, Hinrichs K, Velez IC Tags: Cryobiology Source Type: research

Retrospective analysis of CT-guided percutaneous cryoablation for treatment of painful osteolytic bone metastasis.
This study aimed to evaluate the safety and effectiveness of CT-guided percutaneous cryoablation for treatment of painful osteolytic bone metastases. A total of 26 patients (36 bone metastases) treated with CT-guided percutaneous cryoablation between May 2012 and June 2016 were enrolled in this retrospective study. All procedures were performed under local anesthesia. A visual analog scale (VAS) was used to evaluate pain before the procedure and at 1 day, 1 month, 3 months, and 6 months after the procedure. Complications during and after the procedure were recorded and graded by the Clavien-Dindo classification. The mean V...
Source: Cryobiology - January 17, 2020 Category: Biology Authors: Yang Y, Li Y, Wu Y, Qiu S, Liu C, Wang Q, Hong Y, Lyu J, Zhang Y, Du D Tags: Cryobiology Source Type: research

Cryopreservation of calf testicular tissues with knockout serum replacement.
Abstract To enrich bovine gonocytes from cryopreserved testicular tissues, the cryoprotection effects of the freezing media containing knockout serum replacement (KSR) were examined. Using Minimum essential medium (MEM) + 10% dimethyl sulfoxide (Me2SO) as the basic medium, calf testicular tissues were cryopreserved in media containing 0, 5, 10, 20, 40, 90% KSR and 5% fetal bovine serum (FBS) respectively. Morphologically the seminiferous cords and interstitium were well preserved in all groups. The gonocytes were all glial cell line-derived neurotrophic factor (GDNF) family receptor α-1 (GFRα...
Source: Cryobiology - January 16, 2020 Category: Biology Authors: Jiang Y, Zhu WQ, Zhu XC, Cai NN, Yang R, Cai H, Zhang XM Tags: Cryobiology Source Type: research

Using engineering models to shorten cryoprotectant loading time for the vitrification of articular cartilage.
Abstract Osteochondral allograft transplantation can treat full thickness cartilage and bone lesions in the knee and other joints, but the lack of widespread articular cartilage banking limits the quantity of cartilage available for size and contour matching. To address the limited availability of cartilage, vitrification can be used to store harvested joint tissues indefinitely. Our group's reported vitrification protocol [Biomaterials 33 (2012) 6061-6068] takes 9.5 h to load cryoprotectants into intact articular cartilage on bone and achieves high cell viability, but further optimization is needed to shorten t...
Source: Cryobiology - January 14, 2020 Category: Biology Authors: Shardt N, Chen Z, Yuan SC, Wu K, Laouar L, Jomha NM, Elliott JW Tags: Cryobiology Source Type: research

Late Embryogenesis Abundant (LEA) proteins confer water stress tolerance to mammalian somatic cells.
Abstract Late Embryogenesis Abundant (LEA) proteins are commonly found in plants and other organisms capable of undergoing severe and reversible dehydration, a phenomenon termed "anhydrobiosis". Here, we have produced a tagged version for three different LEA proteins: pTag-RAB17-GFP-N, Zea mays dehydrin-1dhn, expressed in the nucleo-cytoplasm; pTag-WCOR410-RFP, Tricum aestivum cold acclimation protein WCOR410, binds to cellular membranes, and pTag-LEA-BFP, Artemia franciscana LEA protein group 3 that targets the mitochondria. Sheep fibroblasts transfected with single or all three LEA proteins were subjec...
Source: Cryobiology - January 14, 2020 Category: Biology Authors: Czernik M, Fidanza A, Luongo FP, Valbonetti L, Scapolo PA, Patrizio P, Loi P Tags: Cryobiology Source Type: research

Implications of variability in cell membrane permeability for design of methods to remove glycerol from frozen-thawed erythrocytes.
Abstract In North America, red blood cells (RBCs) are currently cryopreserved in a solution of 40% glycerol. While glycerol is not inherently toxic to humans, it must be removed prior to transfusion to prevent intravascular osmotic hemolysis. The current deglycerolization procedure requires about 45 min per RBC unit. We previously presented predictions suggesting that glycerol could be safely removed from RBCs in less than 1 min. However, experimental evaluation of these methods resulted in much higher hemolysis than expected. Here we extend our previous study by considering both concentration-dependence of pe...
Source: Cryobiology - January 11, 2020 Category: Biology Authors: Lahmann JM, Sanchez CC, Benson JD, Acker JP, Higgins AZ Tags: Cryobiology Source Type: research

David E Pegg - Cryobiologist.
PMID: 31930960 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - January 10, 2020 Category: Biology Authors: Rawson D Tags: Cryobiology Source Type: research

Comparison of three multi-cryoprotectant loading protocols for vitrification of porcine articular cartilage.
Abstract Vitrification is a cryopreservation technique for the long-term storage of viable tissue, but the success of this technique relies on multiple factors. In 2012, our group published a working vitrification protocol for intact human articular cartilage and reported promising chondrocyte recovery after using a four-step multi-cryoprotectant (CPA) loading method that required 570 min. However, this protocol requires further optimization for clinical practice. Herein, we compared three multi-step CPA loading protocols to investigate their impact on chondrocyte recovery after vitrification of porcine articula...
Source: Cryobiology - January 6, 2020 Category: Biology Authors: Wu K, Shardt N, Laouar L, Chen Z, Prasad V, Elliott JAW, Jomha NM Tags: Cryobiology Source Type: research

Cumulus cells during in vitro fertilization and oocyte vitrification in sheep: Remove, maintain or add?
The objective was to evaluate the developmental competence of immature and matured ovine oocytes after removing, maintaining or adding cumulus cells (CC) associated to vitrification by Cryotop method. Three experiments were performed involving 3,144 oocytes. In Experiment 1, CC were removed from immature, matured or fertilized oocytes subjected to in vitro embryo production. In Experiment 2, oocytes were vitrified either in MI or MII stage with or without CC, while a control group with CC remained unvitrified. In Experiment 3, oocytes partially denuded from CC were vitrified either in MI or MII stage, and a co-culture of f...
Source: Cryobiology - January 6, 2020 Category: Biology Authors: Dos Santos-Neto PC, Vilariño M, Cuadro F, Barrera N, Crispo M, Menchaca A Tags: Cryobiology Source Type: research

Ginger and echinacea extracts improve the quality and fertility potential of frozen-thawed ram epididymal spermatozoa.
Abstract The current study examined the impact of the supplementation of ginger and echinacea extract, as natural antioxidant agents, in freezing extender on the quality and fertility potential of ram epididymal spermatozoa after cryopreservation. Epididymal spermatozoa isolated from Forty testicles, obtained from 20 rams, with motility>80% and total morphological abnormalities
Source: Cryobiology - December 26, 2019 Category: Biology Authors: Merati Z, Farshad A Tags: Cryobiology Source Type: research

Effect of substituting different concentrations of soybean lecithin and egg yolk in tris-based extender on goat semen cryopreservation.
This study aimed to investigate the effects of different concentrations of soybean lecithin (SL; 0.5%, 1%, and 1.5%) and egg yolk (EY) in Tris-based extenders on the semen quality parameters of post-thawed goat semen. Sixteen ejaculates were collected from eight healthy, mature Chongming White goats (3-5 years of age). Each ejaculate was divided into five equal aliquots, and then each pellet was diluted with one of the five Tris-based extenders containing 20% EY, 0.5% SL, 1% SL, 2% SL, or 3% SL. The cooled diluted semen was loaded into 0.5 mL polyvinyl French straws and cryopreserved in liquid nitrogen. Frozen semen samp...
Source: Cryobiology - December 25, 2019 Category: Biology Authors: Sun L, Fan W, Wu C, Zhang S, Dai J, Zhang D Tags: Cryobiology Source Type: research

The process of bone regeneration from devitalization to revitalization after pedicle freezing with immunohistochemical and histological examination in rabbits.
Abstract The pedicle freezing procedure by liquid nitrogen is a method for the reconstruction of tumor-bearing bone after malignant tumor resection. However, the regenerative mechanism of bone after the pedicle freezing procedure is unclear. We investigated the complete process from devitalization to revitalization of bone after the pedicle freezing procedure in 13 rabbits. After osteotomy the 5 mm distal femurs were immersed in liquid nitrogen, and the specimens were divided into frozen area and sub-frozen area. The bilateral femurs were harvested for evaluation of bone regeneration by histological and immunohi...
Source: Cryobiology - December 22, 2019 Category: Biology Authors: Xu G, Yamamoto N, Nojima T, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Tsuchiya H Tags: Cryobiology Source Type: research

Fertility preservation and preimplantation genetic assessment for women with breast cancer.
Abstract Breast cancer is the most common cancer diagnosed among reproductive aged women, and its treatment can compromise future fertility. Options for fertility preservation include oocyte or embryo cryopreservation after ovarian stimulation (OS), which are the most established choices and are applicable for adult women with cancer. Ovarian tissue freezing may also be appropriate, as it offers potentially the least delay. The recognisation of the role of BRCA1 and BRCA2 mutations in some women has led to the involvement of preimplantation genetic diagnosis (PGD), recently renamed preimplantation genetic testing ...
Source: Cryobiology - December 18, 2019 Category: Biology Authors: Sciorio R, Anderson RA Tags: Cryobiology Source Type: research

A non-activating diluent to prolong in vitro viability of Apis mellifera spermatozoa: Effects on cryopreservation and on egg fertilization.
Abstract A non-activating semen diluent does not cause motility or acrosomal reaction or capacitate the sperm cell. The effects of such a diluent on the viability of honey bee spermatozoa stored in ambient conditions were assessed 60 days pre-cryopreservation and 24 h post-cryopreservation. Seven variations of a Tris-based non-activating diluents (FEM1 - FEM7) were compared to samples treated with conventional activating diluent and untreated semen. Semen viability (membrane integrity) was assessed after short- and long-term storage at 14.0 ± 0.2 °C. The non-activating medium FEM7 contained more...
Source: Cryobiology - November 30, 2019 Category: Biology Authors: Rajamohan A, Danka RG, Hopkins BK, Rinehart JP Tags: Cryobiology Source Type: research

Cryopreservation of heat-shocked canine adipose-derived mesenchymal stromal cells with 10% dimethyl sulfoxide and 40% serum results in better viability, proliferation, anti-oxidation, and in-vitro differentiation.
In this study, fresh-Ad-MSCs were activated with heat shock, hypoxia (5% O2), or hypoxia (5% O2) + heat shock treatments. The results showed that compared to the other treatments, heat shock significantly improved the proliferation rate, anti-oxidation, heat shock proteins and growth factors expressions of canine-fresh-Ad-MSCs. Consequently, fresh-Ad-MSCs were heat-shocked and then cryopreserved with different combinations of dimethyl sulfoxide (Me2SO) and fetal bovine serum (FBS) to determine the combination that could effectively preserve the cellular viability, proliferation, anti-oxidation and differentiation...
Source: Cryobiology - November 27, 2019 Category: Biology Authors: Shahid MA, Kim WH, Kweon OK Tags: Cryobiology Source Type: research

Supplementation of l-tryptophan (an aromatic amino acid) in tris citric acid extender enhances post-thaw progressive motility, plasmalemma, mitochondrial membrane potential, acrosome, and DNA integrities, and in vivo fertility rate of buffalo (Bubalus bubalis) bull spermatozoa.
Abstract The aromatic amino acid l-tryptophan is an essential and versatile molecule, acts by transferring an electron to free radicals and protects the plasma membrane from injuries. The aim of the present study was to investigate the effects of l-tryptophan in extender on semen quality parameters, in vitro longevity and in vivo fertility of buffalo spermatozoa during cryopreservation. Two ejaculates were collected from each bull (n = 2 ejaculates and n = 4 bulls) with artificial vagina at 42 °C followed by initial evaluation for volume, motility, concentrations and were diluted in five extenders (C...
Source: Cryobiology - November 26, 2019 Category: Biology Authors: Ahmed H, Jahan S, Khan A, Khan L, Ullah H, Riaz M, Ullah K, Ullah F Tags: Cryobiology Source Type: research

Total and specific activities of superoxide dismutase (SOD) in seminal plasma are related with the cryotolerance of jackass spermatozoa.
This study investigated whether the activities of four antioxidant enzymes present in jackass seminal plasma (SP), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) and glutathione reductase (GSR), are related to the sperm ability to withstand cryopreservation. Eighteen ejaculates from 16 healthy jackasses were collected and split into two aliquots. The first one was centrifuged (3,000×g, 4 °C for 10 min) and used to determine the activities of these four enzymes in SP, whereas the other was diluted in a skim-milk extender and then cryopreserved. Assessment of sperm motility and memb...
Source: Cryobiology - November 25, 2019 Category: Biology Authors: Papas M, Catalan J, Barranco I, Arroyo L, Bassols A, Yeste M, Miró J Tags: Cryobiology Source Type: research

Cryopreservation produces limited long-term effects on the nematode Caenorhabditis elegans.
Abstract Cryopreservation, the freezing and later warming of biological samples with minimal loss of viability, is important in many scientific disciplines. For some applications, particularly those where there is limited available material, it is critical to ensure the maximal survival rates of cryopreserved materials. Most of the challenges encountered with such techniques take place after the warming process where cryodamage affects cell viability and future development. Here we have used the nematode Caenorhabditis elegans to investigate the effects of cryodamage caused by slow-freezing. We find that freezing ...
Source: Cryobiology - November 23, 2019 Category: Biology Authors: Stastna JJ, Yiapanas AD, Mandawala AA, Fowler KE, Harvey SC Tags: Cryobiology Source Type: research

Recovery and cryopreservation of epididymal sperm from domestic cat using powdered coconut water (ACP-117c) and TRIS extenders.
Abstract Cryopreservation of cats epididymal spermatozoa allows the conservation of the genetic material and the study of the cryogenic effect applied to the gametes of other felines. However, this biotechnique still presents variable results, being necessary the investigation of alternative extenders. Powdered coconut water (ACP-117c) has been efficient in the sperm freezing of several species and in the cat sperm refrigeration. Therefore, we aimed to evaluate the effect of the freezing stages and the quality of the cats' epididymal spermatozoa after thawing, using ACP-117c. Epididymides (n = 36) from 18 cats...
Source: Cryobiology - November 23, 2019 Category: Biology Authors: de Sousa Barbosa B, Izzo RG, Rodrigues Silva HV, Pereira Nunes TG, Brito BF, Pereira Silva TF, Machado da Silva LD Tags: Cryobiology Source Type: research

Clinical utility of freeze-all approach in ART treatment: A mini-review.
Abstract A significant proportion of couples at reproductive age rely on assisted reproductive technology to overcome infertility. In vitro fertilisation (IVF) involves typically the use of exogenous gonadotropins to stimulate the ovary to produce oocytes, which are collected surgically. After fertilization by conventional IVF or intracytoplasmic sperm injection (ICSI), embryos are cultured in the embryology laboratory for a few days before being replaced into the uterus (fresh embryo transfer). Spare embryos can be vitrified and stored in liquid nitrogen to be transferred in a subsequent cycle. Over the years, co...
Source: Cryobiology - November 23, 2019 Category: Biology Authors: Romualdo S, Sandro EC Tags: Cryobiology Source Type: research

Melatonin-related mitochondrial respiration responses are associated with growth promotion and cold tolerance in plants.
The objective of the study is to determine possible effects of melatonin on mitochondrial respiration and energy efficiency in maize leaves grown under optimum temperature and cold stress and to reveal the relationship between melatonin-induced possible alterations in mitochondrial respiration and cold tolerance. Melatonin and cold stress, alone and in combination, caused significant increases in activities and gene expressions of pyruvate dehydrogenase, citrate synthase, and malate dehydrogenase, indicating an acceleration in the rate of tricarboxylic acid cycle. Total mitochondrial respiration rate, cytochrome pathway ra...
Source: Cryobiology - November 20, 2019 Category: Biology Authors: Turk H, Genisel M Tags: Cryobiology Source Type: research

Deep-supercooling for extended preservation of adipose-derived stem cells.
Abstract Cell preservation is an enabling technology for widespread distribution and applications of mammalian cells. Traditional cryopreservation via slow-freezing or vitrification provides long-term storage but requires cytotoxic cryoprotectants (CPA) and tedious CPA loading, cooling, and recovering procedures. Hypothermic storage around 0-4 °C is an alternative method but only works for a short period due to its high storage temperature. Here, we report on the deep-supercooling (DSC) preservation of human adipose-derived stem cells at deep subzero temperatures without freezing for extended storage. Enable...
Source: Cryobiology - November 18, 2019 Category: Biology Authors: Huang H, Bedon CR, Yarmush ML, Usta OB Tags: Cryobiology Source Type: research

Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients.
The objective of this study was to investigate whether mitochondrial antioxidant Mito-Tempo was effective in preventing cryodamage of asthenozoospermic spermatozoa. Asthenozoospermic semen samples were collected and cryopreserved in media supplemented with different concentrations (0.0, 1.0, 10 and 100 μM) of Mito-Tempo. We measured sperm motility, viability, membrane integrity, DNA fragmentation, mitochondrial membrane potential, oxidation product, and antioxidant enzymes activities. Supplementation of the cryopreservation media with Mito-Tempo (10 and 100 μM) induced a significant improvement in sperm viability...
Source: Cryobiology - November 14, 2019 Category: Biology Authors: Zhang X, Lu X, Li J, Xia Q, Gao J, Wu B Tags: Cryobiology Source Type: research

Corrigendum to "Survival, re-expansion, and pregnancy outcome following vitrification of dromedary camel cloned blastocysts: A possible role of vitrification in improving clone pregnancy rate by weeding out poor competent embryos" [Cryobiology 90 (2019) 75-82].
Corrigendum to "Survival, re-expansion, and pregnancy outcome following vitrification of dromedary camel cloned blastocysts: A possible role of vitrification in improving clone pregnancy rate by weeding out poor competent embryos" [Cryobiology 90 (2019) 75-82]. Cryobiology. 2019 Nov 13;: Authors: Moulavi F, Soto-Rodriguez S, Kuwayama M, Asadi-Moghaddam B, Hosseini SM PMID: 31733790 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - November 13, 2019 Category: Biology Authors: Moulavi F, Soto-Rodriguez S, Kuwayama M, Asadi-Moghaddam B, Hosseini SM Tags: Cryobiology Source Type: research

The impact of cryoprotective media on cryopreservation of cells using loading trehalose.
In conclusion, the cryoprotective media impacts on the effectiveness of cryopreservation using loading trehalose. PMID: 31730757 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - November 12, 2019 Category: Biology Authors: Jong KS, Hui YL, Yu CM, Ki SY, Kim SH, Pak HH Tags: Cryobiology Source Type: research

Corrigendum to "Silk protein sericin pretreatment enhances osmotic tolerance and post-thaw sperm quality but reduces the ability of sperm cells to undergo in vitro induced acrosome reaction in rabbit" [Cryo. 90 October (2019) 1-7].
Corrigendum to "Silk protein sericin pretreatment enhances osmotic tolerance and post-thaw sperm quality but reduces the ability of sperm cells to undergo in vitro induced acrosome reaction in rabbit" [Cryo. 90 October (2019) 1-7]. Cryobiology. 2019 Nov 05;: Authors: Raza S, Uçan U, Aksoy M, Erdoğan G, Ceylan A, Serin I PMID: 31703939 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - November 5, 2019 Category: Biology Authors: Raza S, Uçan U, Aksoy M, Erdoğan G, Ceylan A, Serin I Tags: Cryobiology Source Type: research

Influence of different extenders on morphological and functional parameters of frozen-thawed spermatozoa of jaguar (Panthera onca).
Abstract Due to the global decrease in jaguar population, conservation strategies are essential and the development of effective semen cryopreservation protocols would contribute to the formation of germplasm banks. Therefore, the objectives were to (1) evaluate the use of TRIS and ACP-117c extenders for jaguar semen freezing, (2) describe the ultrastructural changes in sperm after cryopreservation, and (3) evaluate the binding capacity of the thawed sperm. Eight ejaculates from five mature individuals were collected by electroejaculation, extended in TRIS or a coconut based-extender (ACP-117c), and frozen in liqu...
Source: Cryobiology - November 5, 2019 Category: Biology Authors: Silva HVR, Nunes TGP, Brito BF, Campos LB, da Silva AM, Silva AR, Comizzoli P, da Silva LDM Tags: Cryobiology Source Type: research

Using natural honey as an anti-oxidant and thermodynamically efficient cryoprotectant in embryo vitrification.
Abstract Embryo cryopreservation is a common practice in reproductive biology and infertility treatments. Despite major improvements over years, the cryoprotectant solutions are still a major source of concern, mostly due to their chemical toxicity and suboptimal protection against cryoinjuries. In this work, we introduced natural honey as a non-permeating cryoprotectant to replace traditionally used sucrose in embryo vitrification. The proposed media were compared with conventional ones by evaluating vitrified/warmed mouse embryos based on their re-expansion, hatching rate and transcription pattern of selected ge...
Source: Cryobiology - November 4, 2019 Category: Biology Authors: Sarmadi F, Kazemi P, Tirgar P, Fayazi S, Esfandiari S, Sotoodeh L, Molaeian S, Dashtizad M Tags: Cryobiology Source Type: research

Natural cryoprotectants combinations of l-proline and trehalose for red blood cells cryopreservation.
Abstract Cryopreservation of red blood cells (RBCs) holds great potential benefits for supplying transfusion timely in emergencies. Currently, glycerol is the main cryoprotectant permitted in clinical therapy for RBCs cryopreservation, but its broad application is limited by the toxicity and complex deglycerolization process. Successful cryopreservation of RBCs using more effective materials should be studied to reduce freezing damage, increase biocompatibility, and save processing time. Herein, a simple protocol using natural cryoprotectants combinations of l-proline and trehalose attains a low degree of hemolysi...
Source: Cryobiology - November 3, 2019 Category: Biology Authors: Dou M, Lu C, Sun Z, Rao W Tags: Cryobiology Source Type: research

Combination of intracellular cryoprotectants preserves the structure and the cells proliferative capacity potential of adult collared peccary testicular tissue subjected to solid surface vitrification.
The objective was to evaluate different permeating cryoprotectants to vitrify testicular tissue biopsies from adult collared peccaries. Five pairs of testicles were dissected into fragments (9 mm³) that were allocated to non-vitrified (control) and vitrified groups using a solid-surface method following exposure to different cryoprotectants (3.0 M dimethyl sulfoxide (DMSO), 3.0 M ethylene glycol (EG) or 1.5 M DMSO + 1.5 M EG). After warming, samples were evaluated for histomorphology, ultrastructure, viability, and proliferative capacity potential. The appropriate conservation o...
Source: Cryobiology - October 31, 2019 Category: Biology Authors: da Silva AM, Bezerra LGP, Praxedes ECG, Moreira SSJ, de Souza CMP, de Oliveira MF, Pereira AF, Comizzoli P, Silva AR Tags: Cryobiology Source Type: research

"Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm''.
"Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm''. Cryobiology. 2019 Oct 31;: Authors: Arslan HO, Herrera C, Malama E, Siuda M, Leiding C, Bollwein H Abstract The aim of this study was to investigate the effects of different concentrations of catalase in a TRIS-egg yolk extender on sperm quality and embryonic development after in vitro fertilization of frozen-thawed bull sperm. For this purpose, from each of 7 bulls 2 ejaculates were collected and diluted with a TRIS-egg yolk extender c...
Source: Cryobiology - October 31, 2019 Category: Biology Authors: Arslan HO, Herrera C, Malama E, Siuda M, Leiding C, Bollwein H Tags: Cryobiology Source Type: research

Time-resolved Fluorescence and Generalized Polarization: Innovative tools to assess bull sperm membrane dynamics during slow freezing.
Abstract During slow freezing, spermatozoa undergo membrane alterations that compromise their ability of fertilizing. These alterations are cause either by cold shock or by the use of cryoprotectants known to be cytotoxic. However, little is known about the membrane changes that occurred during freezing. Here, we combined Generalized Polarization (GP), Time-resolved Fluorescence and laurdan fluorescence properties to investigate the changes in membrane fluidity and dynamics during slow freezing of bull sperm. We successfully demonstrated that laurdan may be distributed in three different local environments that co...
Source: Cryobiology - October 30, 2019 Category: Biology Authors: Bechoua S, Winckler P, Jossier A, Peltier C, Delize F, Devaux N, Perrier Cornet JM, Simonin H Tags: Cryobiology Source Type: research

Effects of supplementation of tris-egg yolk extender with different sugars and antioxidants on freezability of ram semen.
In conclusion, the addition of 100 mM trehalose, 60 mM sucrose and 10 mM raffinose combined with 2 mM vitamin E in Tris-egg yolk extender significantly improved frozen-thawed ram semen parameters. PMID: 31669434 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - October 26, 2019 Category: Biology Authors: Rostami B, Ebrahimi D, Sadeghipanah H, Masoumi R, Shahir MH Tags: Cryobiology Source Type: research

Cold tolerance and supercooling points of two ladybird beetles (Col.: Coccinellidae): Impact of the diet.
Abstract Ladybird beetles have successfully been used to control different pests. Cheilomenes sexmaculata (Fabricius) and Oenopia conglobata contaminata)Menetries((Coleoptera: Coccinellidae) are two dominant and efficient predators of the common pistachio psylla, Agonoscena pistaciae (Hem.: Psyllidae) in Iran. In the current study, the impact of two diets, i.e., nymphs of A. pistaciae and eggs of Ephestia kuehniella Zeller (Lep.: Pyralidae), were investigated on the cold hardiness, supercooling point (SCP), and lethal temperature of different life stages of the coccinellids. The results suggested that the eggs of ...
Source: Cryobiology - October 24, 2019 Category: Biology Authors: Pourani MS, Mahdian K, Izadi H, Basirat M, Sahhafi SR Tags: Cryobiology Source Type: research

In Memoriam - David E Pegg, MD. FRC Path.
Authors: PMID: 31654624 [PubMed - as supplied by publisher] (Source: Cryobiology)
Source: Cryobiology - October 22, 2019 Category: Biology Tags: Cryobiology Source Type: research

Vitrification of collared peccary ovarian tissue using open or closed systems and different intracellular cryoprotectants.
This study aimed to evaluate different vitrification methods using distinct cryoprotectants (CPAs) for the preservation of collared peccary ovarian preantral follicles (PFs). Ovarian pairs from six females were fragmented and three fragments (fresh control group) were immediately evaluated for morphology, viability, cell proliferation capacity (assessed by quantifying the number of argyrophilic nucleolus organizer regions - NORs), and apoptosis (by the identification of activated caspase-3 expression). The remaining 18 fragments were vitrified using the solid surface vitrification (SSV) method or the ovarian tissue cryosys...
Source: Cryobiology - October 19, 2019 Category: Biology Authors: Campos LB, da Silva AM, Praxedes ECG, Bezerra LGP, Gama Lins TLB, Menezes VG, de Matos MHT, Lima GL, Rodrigues APR, Silva AR Tags: Cryobiology Source Type: research