Vitrification of manually stage-selected embryos of Drosophila have significantly higher survival and emergence - Consequences for insect germplasm storage
In this study, the effect of embryo developmental temperature (19, 20 and 21 °C) on the stage specific convergence of the embryonic development to the developmental stages 15-17, that is useful for cryopreservation, was studied in a flightless mutant strain of Drosophila melanogaster and compared with the Ore-R strain. The temperature that allowed for the best convergence to stage 16 was chosen for further selection and treatment of the embryos. The converged embryos (SS) were directly treated or further manually sorted (MS) for the requisite developmental stage to reduce the number of non-convergent embryos. These sel...
Source: Cryobiology - January 15, 2022 Category: Biology Authors: Korie M DeBardlabon Arun Rajamohan Joseph P Rinehart Source Type: research

Vitrification of manually stage-selected embryos of Drosophila have significantly higher survival and emergence - Consequences for insect germplasm storage
In this study, the effect of embryo developmental temperature (19, 20 and 21 °C) on the stage specific convergence of the embryonic development to the developmental stages 15-17, that is useful for cryopreservation, was studied in a flightless mutant strain of Drosophila melanogaster and compared with the Ore-R strain. The temperature that allowed for the best convergence to stage 16 was chosen for further selection and treatment of the embryos. The converged embryos (SS) were directly treated or further manually sorted (MS) for the requisite developmental stage to reduce the number of non-convergent embryos. These sel...
Source: Cryobiology - January 15, 2022 Category: Biology Authors: Korie M DeBardlabon Arun Rajamohan Joseph P Rinehart Source Type: research

Changes of pollen viability after preservation of Paeonia lactiflora in different provenances
This study compared the viability and physiological condition of Paeonia lactiflora pollen from several provenances after preservation, to explore the effect of provenance difference on pollen viability and physiological responses after preservation. The results showed that: the pollen viability of two cultivars were significantly different in provenances after preserved at -20 °C or liquid nitrogen (LN) for 3 months, the pollen viability of 'Fen Yu Nu' showed Lanzhou> Beijing> Luoyang> Heze, while the pollen viability of 'Zi Feng Chao Yang' showed Luoyang> Beijing> Heze. Similarly, the oxidative stress ...
Source: Cryobiology - January 6, 2022 Category: Biology Authors: Ruifen Ren Jianzhou Gao Hao Zhou Mengting Zhu Yan Liu Source Type: research

Changes of pollen viability after preservation of Paeonia lactiflora in different provenances
This study compared the viability and physiological condition of Paeonia lactiflora pollen from several provenances after preservation, to explore the effect of provenance difference on pollen viability and physiological responses after preservation. The results showed that: the pollen viability of two cultivars were significantly different in provenances after preserved at -20 °C or liquid nitrogen (LN) for 3 months, the pollen viability of 'Fen Yu Nu' showed Lanzhou> Beijing> Luoyang> Heze, while the pollen viability of 'Zi Feng Chao Yang' showed Luoyang> Beijing> Heze. Similarly, the oxidative stress ...
Source: Cryobiology - January 6, 2022 Category: Biology Authors: Ruifen Ren Jianzhou Gao Hao Zhou Mengting Zhu Yan Liu Source Type: research

Changes of pollen viability after preservation of Paeonia lactiflora in different provenances
This study compared the viability and physiological condition of Paeonia lactiflora pollen from several provenances after preservation, to explore the effect of provenance difference on pollen viability and physiological responses after preservation. The results showed that: the pollen viability of two cultivars were significantly different in provenances after preserved at -20 °C or liquid nitrogen (LN) for 3 months, the pollen viability of 'Fen Yu Nu' showed Lanzhou> Beijing> Luoyang> Heze, while the pollen viability of 'Zi Feng Chao Yang' showed Luoyang> Beijing> Heze. Similarly, the oxidative stress ...
Source: Cryobiology - January 6, 2022 Category: Biology Authors: Ruifen Ren Jianzhou Gao Hao Zhou Mengting Zhu Yan Liu Source Type: research

Changes of pollen viability after preservation of Paeonia lactiflora in different provenances
This study compared the viability and physiological condition of Paeonia lactiflora pollen from several provenances after preservation, to explore the effect of provenance difference on pollen viability and physiological responses after preservation. The results showed that: the pollen viability of two cultivars were significantly different in provenances after preserved at -20 °C or liquid nitrogen (LN) for 3 months, the pollen viability of 'Fen Yu Nu' showed Lanzhou> Beijing> Luoyang> Heze, while the pollen viability of 'Zi Feng Chao Yang' showed Luoyang> Beijing> Heze. Similarly, the oxidative stress ...
Source: Cryobiology - January 6, 2022 Category: Biology Authors: Ruifen Ren Jianzhou Gao Hao Zhou Mengting Zhu Yan Liu Source Type: research

Changes of pollen viability after preservation of Paeonia lactiflora in different provenances
This study compared the viability and physiological condition of Paeonia lactiflora pollen from several provenances after preservation, to explore the effect of provenance difference on pollen viability and physiological responses after preservation. The results showed that: the pollen viability of two cultivars were significantly different in provenances after preserved at -20 °C or liquid nitrogen (LN) for 3 months, the pollen viability of 'Fen Yu Nu' showed Lanzhou> Beijing> Luoyang> Heze, while the pollen viability of 'Zi Feng Chao Yang' showed Luoyang> Beijing> Heze. Similarly, the oxidative stress ...
Source: Cryobiology - January 6, 2022 Category: Biology Authors: Ruifen Ren Jianzhou Gao Hao Zhou Mengting Zhu Yan Liu Source Type: research

Changes of pollen viability after preservation of Paeonia lactiflora in different provenances
This study compared the viability and physiological condition of Paeonia lactiflora pollen from several provenances after preservation, to explore the effect of provenance difference on pollen viability and physiological responses after preservation. The results showed that: the pollen viability of two cultivars were significantly different in provenances after preserved at -20 °C or liquid nitrogen (LN) for 3 months, the pollen viability of 'Fen Yu Nu' showed Lanzhou> Beijing> Luoyang> Heze, while the pollen viability of 'Zi Feng Chao Yang' showed Luoyang> Beijing> Heze. Similarly, the oxidative stress ...
Source: Cryobiology - January 6, 2022 Category: Biology Authors: Ruifen Ren Jianzhou Gao Hao Zhou Mengting Zhu Yan Liu Source Type: research

Development of assisted reproductive technologies for the conservation of Atelopus sp. (spumarius complex)
The objective of this study was to apply ARTs in sexually mature individuals of an undescribed species of Atelopus (spumarius complex) (harlequin frog). Our procedure involves hormonal induction of gametogenesis in this species. We were able to induce gamete release through administration of human chorionic gonadotropin (hCG) in males, and in females this has been achieved through the sequential administration of hCG (priming doses), and combinations of hCG with gonadotropin releasing hormone analogue, GnRHa (ovulary dose). We standardized sperm cryopreservation by performing toxicity tests of cryoprotectants, fast/slow fr...
Source: Cryobiology - December 30, 2021 Category: Biology Authors: Renato E Naranjo Elena Naydenova Carolina Proa ño-Bolaños Karla Vizuete Alexis Debut Marbel Torres Arias Luis A Coloma Source Type: research

Development of assisted reproductive technologies for the conservation of Atelopus sp. (spumarius complex)
The objective of this study was to apply ARTs in sexually mature individuals of an undescribed species of Atelopus (spumarius complex) (harlequin frog). Our procedure involves hormonal induction of gametogenesis in this species. We were able to induce gamete release through administration of human chorionic gonadotropin (hCG) in males, and in females this has been achieved through the sequential administration of hCG (priming doses), and combinations of hCG with gonadotropin releasing hormone analogue, GnRHa (ovulary dose). We standardized sperm cryopreservation by performing toxicity tests of cryoprotectants, fast/slow fr...
Source: Cryobiology - December 30, 2021 Category: Biology Authors: Renato E Naranjo Elena Naydenova Carolina Proa ño-Bolaños Karla Vizuete Alexis Debut Marbel Torres Arias Luis A Coloma Source Type: research

Development of assisted reproductive technologies for the conservation of Atelopus sp. (spumarius complex)
The objective of this study was to apply ARTs in sexually mature individuals of an undescribed species of Atelopus (spumarius complex) (harlequin frog). Our procedure involves hormonal induction of gametogenesis in this species. We were able to induce gamete release through administration of human chorionic gonadotropin (hCG) in males, and in females this has been achieved through the sequential administration of hCG (priming doses), and combinations of hCG with gonadotropin releasing hormone analogue, GnRHa (ovulary dose). We standardized sperm cryopreservation by performing toxicity tests of cryoprotectants, fast/slow fr...
Source: Cryobiology - December 30, 2021 Category: Biology Authors: Renato E Naranjo Elena Naydenova Carolina Proa ño-Bolaños Karla Vizuete Alexis Debut Marbel Torres Arias Luis A Coloma Source Type: research

Development of assisted reproductive technologies for the conservation of Atelopus sp. (spumarius complex)
The objective of this study was to apply ARTs in sexually mature individuals of an undescribed species of Atelopus (spumarius complex) (harlequin frog). Our procedure involves hormonal induction of gametogenesis in this species. We were able to induce gamete release through administration of human chorionic gonadotropin (hCG) in males, and in females this has been achieved through the sequential administration of hCG (priming doses), and combinations of hCG with gonadotropin releasing hormone analogue, GnRHa (ovulary dose). We standardized sperm cryopreservation by performing toxicity tests of cryoprotectants, fast/slow fr...
Source: Cryobiology - December 30, 2021 Category: Biology Authors: Renato E Naranjo Elena Naydenova Carolina Proa ño-Bolaños Karla Vizuete Alexis Debut Marbel Torres Arias Luis A Coloma Source Type: research

Effect of two preservation methods on the viability and enzyme production of a recombinant Komagataella phaffii (Pichia pastoris) strain
In this study, we evaluated the effect of glycerol and skim milk in cryopreservation at -80 °C, as well as the use of skim milk or its combination with NaCl, disaccharides or sorbitol in freeze-drying on the cell viability and activity of a recombinant lysosomal enzyme (i.e., human β-hexosaminidase-A) produced in K. phaffii GS115 strain. The results showed that cryopreservation with glycerol and skim milk, as well as freeze-drying using disaccharides and sorbitol with skim milk, maintained the viability above 80%. Although variations in enzyme activity among treatments were found, the use of disaccharides had a po...
Source: Cryobiology - December 24, 2021 Category: Biology Authors: Angela Mar ía Alvarado-Fernández Edwin Alexander Rodr íguez-López Angela Johana Espejo-Mojica Angela Roc ío Mosquera-Arévalo Carlos Javier Alm éciga-Díaz Alba Alicia Trespalacios-Rangel Source Type: research

Effect of two preservation methods on the viability and enzyme production of a recombinant Komagataella phaffii (Pichia pastoris) strain
In this study, we evaluated the effect of glycerol and skim milk in cryopreservation at -80 °C, as well as the use of skim milk or its combination with NaCl, disaccharides or sorbitol in freeze-drying on the cell viability and activity of a recombinant lysosomal enzyme (i.e., human β-hexosaminidase-A) produced in K. phaffii GS115 strain. The results showed that cryopreservation with glycerol and skim milk, as well as freeze-drying using disaccharides and sorbitol with skim milk, maintained the viability above 80%. Although variations in enzyme activity among treatments were found, the use of disaccharides had a po...
Source: Cryobiology - December 24, 2021 Category: Biology Authors: Angela Mar ía Alvarado-Fernández Edwin Alexander Rodr íguez-López Angela Johana Espejo-Mojica Angela Roc ío Mosquera-Arévalo Carlos Javier Alm éciga-Díaz Alba Alicia Trespalacios-Rangel Source Type: research

Effect of two preservation methods on the viability and enzyme production of a recombinant Komagataella phaffii (Pichia pastoris) strain
In this study, we evaluated the effect of glycerol and skim milk in cryopreservation at -80 °C, as well as the use of skim milk or its combination with NaCl, disaccharides or sorbitol in freeze-drying on the cell viability and activity of a recombinant lysosomal enzyme (i.e., human β-hexosaminidase-A) produced in K. phaffii GS115 strain. The results showed that cryopreservation with glycerol and skim milk, as well as freeze-drying using disaccharides and sorbitol with skim milk, maintained the viability above 80%. Although variations in enzyme activity among treatments were found, the use of disaccharides had a po...
Source: Cryobiology - December 24, 2021 Category: Biology Authors: Angela Mar ía Alvarado-Fernández Edwin Alexander Rodr íguez-López Angela Johana Espejo-Mojica Angela Roc ío Mosquera-Arévalo Carlos Javier Alm éciga-Díaz Alba Alicia Trespalacios-Rangel Source Type: research

Effect of two preservation methods on the viability and enzyme production of a recombinant Komagataella phaffii (Pichia pastoris) strain
In this study, we evaluated the effect of glycerol and skim milk in cryopreservation at -80 °C, as well as the use of skim milk or its combination with NaCl, disaccharides or sorbitol in freeze-drying on the cell viability and activity of a recombinant lysosomal enzyme (i.e., human β-hexosaminidase-A) produced in K. phaffii GS115 strain. The results showed that cryopreservation with glycerol and skim milk, as well as freeze-drying using disaccharides and sorbitol with skim milk, maintained the viability above 80%. Although variations in enzyme activity among treatments were found, the use of disaccharides had a po...
Source: Cryobiology - December 24, 2021 Category: Biology Authors: Alvarado-Fern ández Angela María Edwin Alexander Rodr íguez-López Angela Johana Espejo-Mojica Angela Roc ío Mosquera-Arévalo Carlos Javier Alm éciga-Díaz Alba Alicia Trespalacios-Rangel Source Type: research

Effect of two preservation methods on the viability and enzyme production of a recombinant Komagataella phaffii (Pichia pastoris) strain
In this study, we evaluated the effect of glycerol and skim milk in cryopreservation at -80 °C, as well as the use of skim milk or its combination with NaCl, disaccharides or sorbitol in freeze-drying on the cell viability and activity of a recombinant lysosomal enzyme (i.e., human β-hexosaminidase-A) produced in K. phaffii GS115 strain. The results showed that cryopreservation with glycerol and skim milk, as well as freeze-drying using disaccharides and sorbitol with skim milk, maintained the viability above 80%. Although variations in enzyme activity among treatments were found, the use of disaccharides had a po...
Source: Cryobiology - December 24, 2021 Category: Biology Authors: Alvarado-Fern ández Angela María Edwin Alexander Rodr íguez-López Angela Johana Espejo-Mojica Angela Roc ío Mosquera-Arévalo Carlos Javier Alm éciga-Díaz Alba Alicia Trespalacios-Rangel Source Type: research

Cryopreservation of network bioactivity and multi-lineage stromal cell differentiation potential within three-dimensional synthetic hydrogels
Cryobiology. 2021 Dec 16:S0011-2240(21)00374-6. doi: 10.1016/j.cryobiol.2021.12.003. Online ahead of print.ABSTRACTDespite progress in many aspects of controlling cell behavior within synthetic three-dimensional hydrogels, approaches to cryopreserve these systems - encompassing the protection of both encapsulated cell viability and network bioactive functions - are lacking. Here, we demonstrate the retention of encapsulated human mesenchymal stromal cell (hMSC) viability following in situ cryopreservation regardless of cell line, material system, or storage duration. Further, the preservation extends to network bioactive f...
Source: Cryobiology - December 19, 2021 Category: Biology Authors: Sudhir Khetan Source Type: research

Cryopreservation of network bioactivity and multi-lineage stromal cell differentiation potential within three-dimensional synthetic hydrogels
Cryobiology. 2021 Dec 16:S0011-2240(21)00374-6. doi: 10.1016/j.cryobiol.2021.12.003. Online ahead of print.ABSTRACTDespite progress in many aspects of controlling cell behavior within synthetic three-dimensional hydrogels, approaches to cryopreserve these systems - encompassing the protection of both encapsulated cell viability and network bioactive functions - are lacking. Here, we demonstrate the retention of encapsulated human mesenchymal stromal cell (hMSC) viability following in situ cryopreservation regardless of cell line, material system, or storage duration. Further, the preservation extends to network bioactive f...
Source: Cryobiology - December 19, 2021 Category: Biology Authors: Sudhir Khetan Source Type: research

Cryopreservation of network bioactivity and multi-lineage stromal cell differentiation potential within three-dimensional synthetic hydrogels
Cryobiology. 2021 Dec 16:S0011-2240(21)00374-6. doi: 10.1016/j.cryobiol.2021.12.003. Online ahead of print.ABSTRACTDespite progress in many aspects of controlling cell behavior within synthetic three-dimensional hydrogels, approaches to cryopreserve these systems - encompassing the protection of both encapsulated cell viability and network bioactive functions - are lacking. Here, we demonstrate the retention of encapsulated human mesenchymal stromal cell (hMSC) viability following in situ cryopreservation regardless of cell line, material system, or storage duration. Further, the preservation extends to network bioactive f...
Source: Cryobiology - December 19, 2021 Category: Biology Authors: Sudhir Khetan Source Type: research

Cryopreservation of network bioactivity and multi-lineage stromal cell differentiation potential within three-dimensional synthetic hydrogels
Cryobiology. 2021 Dec 16:S0011-2240(21)00374-6. doi: 10.1016/j.cryobiol.2021.12.003. Online ahead of print.ABSTRACTDespite progress in many aspects of controlling cell behavior within synthetic three-dimensional hydrogels, approaches to cryopreserve these systems - encompassing the protection of both encapsulated cell viability and network bioactive functions - are lacking. Here, we demonstrate the retention of encapsulated human mesenchymal stromal cell (hMSC) viability following in situ cryopreservation regardless of cell line, material system, or storage duration. Further, the preservation extends to network bioactive f...
Source: Cryobiology - December 19, 2021 Category: Biology Authors: Sudhir Khetan Source Type: research

Cryopreservation of network bioactivity and multi-lineage stromal cell differentiation potential within three-dimensional synthetic hydrogels
Cryobiology. 2021 Dec 16:S0011-2240(21)00374-6. doi: 10.1016/j.cryobiol.2021.12.003. Online ahead of print.ABSTRACTDespite progress in many aspects of controlling cell behavior within synthetic three-dimensional hydrogels, approaches to cryopreserve these systems - encompassing the protection of both encapsulated cell viability and network bioactive functions - are lacking. Here, we demonstrate the retention of encapsulated human mesenchymal stromal cell (hMSC) viability following in situ cryopreservation regardless of cell line, material system, or storage duration. Further, the preservation extends to network bioactive f...
Source: Cryobiology - December 19, 2021 Category: Biology Authors: Sudhir Khetan Source Type: research

Transplantation of rat frozen-thawed testicular tissues: Does fragment size matter?
The objective of this study is to detect the effect of varying tissue sizes on the efficacy of rat testicular tissue cryopreservation and transplantation. Varying sizes of rat testicular tissues were frozen-thawed and autografted. At the 30th day after grafting, the grafts were collected for histology assessment and immunohistochemistry assay for MAGE-A4 (germ cell marker) and CD34 (blood vessel marker). The transplant recovery, seminiferous tubule integrity, tubular diameter, spermatogonia number, and microsvessel density in testicular fragments sizing in 3 mm in length, 3 mm wide, and 3 mm in thickness were significantly...
Source: Cryobiology - December 17, 2021 Category: Biology Authors: Hong-Xia Wang Xi-Lan Lu Jun-Tao Li Jian-Min Zhang Source Type: research

Transplantation of rat frozen-thawed testicular tissues: Does fragment size matter?
The objective of this study is to detect the effect of varying tissue sizes on the efficacy of rat testicular tissue cryopreservation and transplantation. Varying sizes of rat testicular tissues were frozen-thawed and autografted. At the 30th day after grafting, the grafts were collected for histology assessment and immunohistochemistry assay for MAGE-A4 (germ cell marker) and CD34 (blood vessel marker). The transplant recovery, seminiferous tubule integrity, tubular diameter, spermatogonia number, and microsvessel density in testicular fragments sizing in 3 mm in length, 3 mm wide, and 3 mm in thickness were significantly...
Source: Cryobiology - December 17, 2021 Category: Biology Authors: Hong-Xia Wang Xi-Lan Lu Jun-Tao Li Jian-Min Zhang Source Type: research

A triple stain method in conjunction with an in-depth screening of cryopreservation effects on post-thaw sperm in dogs
In conclusion, cryopreservation has a broad impact in sperm morphology and function, altering motility patterns, plasma, acrosome and mitochondrial membranes integrity, as well as sperm DNA.PMID:34902341 | DOI:10.1016/j.cryobiol.2021.12.001 (Source: Cryobiology)
Source: Cryobiology - December 13, 2021 Category: Biology Authors: Daniel de Souza Ramos Angrimani Luana de C ássia Bicudo Nuria Llamas Luceno Bart Leemans Marcilio Nichi Camila Infantosi Vannucchi Ann Van Soom Source Type: research

A triple stain method in conjunction with an in-depth screening of cryopreservation effects on post-thaw sperm in dogs
In conclusion, cryopreservation has a broad impact in sperm morphology and function, altering motility patterns, plasma, acrosome and mitochondrial membranes integrity, as well as sperm DNA.PMID:34902341 | DOI:10.1016/j.cryobiol.2021.12.001 (Source: Cryobiology)
Source: Cryobiology - December 13, 2021 Category: Biology Authors: Daniel de Souza Ramos Angrimani Luana de C ássia Bicudo Nuria Llamas Luceno Bart Leemans Marcilio Nichi Camila Infantosi Vannucchi Ann Van Soom Source Type: research

A triple stain method in conjunction with an in-depth screening of cryopreservation effects on post-thaw sperm in dogs
In conclusion, cryopreservation has a broad impact in sperm morphology and function, altering motility patterns, plasma, acrosome and mitochondrial membranes integrity, as well as sperm DNA.PMID:34902341 | DOI:10.1016/j.cryobiol.2021.12.001 (Source: Cryobiology)
Source: Cryobiology - December 13, 2021 Category: Biology Authors: Daniel de Souza Ramos Angrimani Luana de C ássia Bicudo Nuria Llamas Luceno Bart Leemans Marcilio Nichi Camila Infantosi Vannucchi Ann Van Soom Source Type: research

A triple stain method in conjunction with an in-depth screening of cryopreservation effects on post-thaw sperm in dogs
In conclusion, cryopreservation has a broad impact in sperm morphology and function, altering motility patterns, plasma, acrosome and mitochondrial membranes integrity, as well as sperm DNA.PMID:34902341 | DOI:10.1016/j.cryobiol.2021.12.001 (Source: Cryobiology)
Source: Cryobiology - December 13, 2021 Category: Biology Authors: Daniel de Souza Ramos Angrimani Luana de C ássia Bicudo Nuria Llamas Luceno Bart Leemans Marcilio Nichi Camila Infantosi Vannucchi Ann Van Soom Source Type: research

The alleviative effect of thyroid hormone on cold stress-induced apotosis via HSP70 and mitochondrial apoptosis signal pathway in bovine Sertoli cells
Cryobiology. 2021 Dec 1:S0011-2240(21)00369-2. doi: 10.1016/j.cryobiol.2021.11.181. Online ahead of print.ABSTRACTThyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 c...
Source: Cryobiology - December 5, 2021 Category: Biology Authors: Han Zhang He Huang Peng Zheng Rui Feng Xue Wang Fushuo Huang Mingjun Ma Yaguang Tian Guixue Zhang Source Type: research

The alleviative effect of thyroid hormone on cold stress-induced apotosis via HSP70 and mitochondrial apoptosis signal pathway in bovine Sertoli cells
Cryobiology. 2021 Dec 1:S0011-2240(21)00369-2. doi: 10.1016/j.cryobiol.2021.11.181. Online ahead of print.ABSTRACTThyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 c...
Source: Cryobiology - December 5, 2021 Category: Biology Authors: Han Zhang He Huang Peng Zheng Rui Feng Xue Wang Fushuo Huang Mingjun Ma Yaguang Tian Guixue Zhang Source Type: research

The alleviative effect of thyroid hormone on cold stress-induced apotosis via HSP70 and mitochondrial apoptosis signal pathway in bovine Sertoli cells
Cryobiology. 2021 Dec 1:S0011-2240(21)00369-2. doi: 10.1016/j.cryobiol.2021.11.181. Online ahead of print.ABSTRACTThyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 c...
Source: Cryobiology - December 5, 2021 Category: Biology Authors: Han Zhang He Huang Peng Zheng Rui Feng Xue Wang Fushuo Huang Mingjun Ma Yaguang Tian Guixue Zhang Source Type: research

The alleviative effect of thyroid hormone on cold stress-induced apotosis via HSP70 and mitochondrial apoptosis signal pathway in bovine Sertoli cells
Cryobiology. 2021 Dec 1:S0011-2240(21)00369-2. doi: 10.1016/j.cryobiol.2021.11.181. Online ahead of print.ABSTRACTThyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 c...
Source: Cryobiology - December 5, 2021 Category: Biology Authors: Han Zhang He Huang Peng Zheng Rui Feng Xue Wang Fushuo Huang Mingjun Ma Yaguang Tian Guixue Zhang Source Type: research

The alleviative effect of thyroid hormone on cold stress-induced apotosis via HSP70 and mitochondrial apoptosis signal pathway in bovine Sertoli cells
Cryobiology. 2021 Dec 1:S0011-2240(21)00369-2. doi: 10.1016/j.cryobiol.2021.11.181. Online ahead of print.ABSTRACTThyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 c...
Source: Cryobiology - December 5, 2021 Category: Biology Authors: Han Zhang He Huang Peng Zheng Rui Feng Xue Wang Fushuo Huang Mingjun Ma Yaguang Tian Guixue Zhang Source Type: research

The alleviative effect of thyroid hormone on cold stress-induced apotosis via HSP70 and mitochondrial apoptosis signal pathway in bovine Sertoli cells
Cryobiology. 2021 Dec 1:S0011-2240(21)00369-2. doi: 10.1016/j.cryobiol.2021.11.181. Online ahead of print.ABSTRACTThyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 c...
Source: Cryobiology - December 5, 2021 Category: Biology Authors: Han Zhang He Huang Peng Zheng Rui Feng Xue Wang Fushuo Huang Mingjun Ma Yaguang Tian Guixue Zhang Source Type: research

The alleviative effect of thyroid hormone on cold stress-induced apotosis via HSP70 and mitochondrial apoptosis signal pathway in bovine Sertoli cells
Cryobiology. 2021 Dec 1:S0011-2240(21)00369-2. doi: 10.1016/j.cryobiol.2021.11.181. Online ahead of print.ABSTRACTThyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 c...
Source: Cryobiology - December 5, 2021 Category: Biology Authors: Han Zhang He Huang Peng Zheng Rui Feng Xue Wang Fushuo Huang Mingjun Ma Yaguang Tian Guixue Zhang Source Type: research

Effect of cryoprotectants on rat kidney decellularization by freeze-thaw process
This study is focused on the effects of a freeze-thaw process and cryoprotectants on the ultrastructure and composition of decellularization scaffolds. Results showed that cryoprotectants and freezing temperatures had significant effects on the decellularization scaffold. The vascular network integrity at -20 °C was better than that at -80 °C. For low-concentration cryoprotectants, 10% dimethyl sulfoxide and 5% trehalose could achieve a better balance between preserving the vascular tree and decellularization. For high-concentration cryoprotectants (vitrification solutions VS55 and VS83), the vascular network integ...
Source: Cryobiology - December 1, 2021 Category: Biology Authors: Jiamin Yang Yi Xu Sichang Luo Hangyu Dang Mengyuan Cao Source Type: research

Effect of cryoprotectants on rat kidney decellularization by freeze-thaw process
This study is focused on the effects of a freeze-thaw process and cryoprotectants on the ultrastructure and composition of decellularization scaffolds. Results showed that cryoprotectants and freezing temperatures had significant effects on the decellularization scaffold. The vascular network integrity at -20 °C was better than that at -80 °C. For low-concentration cryoprotectants, 10% dimethyl sulfoxide and 5% trehalose could achieve a better balance between preserving the vascular tree and decellularization. For high-concentration cryoprotectants (vitrification solutions VS55 and VS83), the vascular network integ...
Source: Cryobiology - December 1, 2021 Category: Biology Authors: Jiamin Yang Yi Xu Sichang Luo Hangyu Dang Mengyuan Cao Source Type: research

Effect of cryoprotectants on rat kidney decellularization by freeze-thaw process
This study is focused on the effects of a freeze-thaw process and cryoprotectants on the ultrastructure and composition of decellularization scaffolds. Results showed that cryoprotectants and freezing temperatures had significant effects on the decellularization scaffold. The vascular network integrity at -20 °C was better than that at -80 °C. For low-concentration cryoprotectants, 10% dimethyl sulfoxide and 5% trehalose could achieve a better balance between preserving the vascular tree and decellularization. For high-concentration cryoprotectants (vitrification solutions VS55 and VS83), the vascular network integ...
Source: Cryobiology - December 1, 2021 Category: Biology Authors: Jiamin Yang Yi Xu Sichang Luo Hangyu Dang Mengyuan Cao Source Type: research

Effect of cryoprotectants on rat kidney decellularization by freeze-thaw process
This study is focused on the effects of a freeze-thaw process and cryoprotectants on the ultrastructure and composition of decellularization scaffolds. Results showed that cryoprotectants and freezing temperatures had significant effects on the decellularization scaffold. The vascular network integrity at -20 °C was better than that at -80 °C. For low-concentration cryoprotectants, 10% dimethyl sulfoxide and 5% trehalose could achieve a better balance between preserving the vascular tree and decellularization. For high-concentration cryoprotectants (vitrification solutions VS55 and VS83), the vascular network integ...
Source: Cryobiology - December 1, 2021 Category: Biology Authors: Jiamin Yang Yi Xu Sichang Luo Hangyu Dang Mengyuan Cao Source Type: research

Cryoprotectant-free preservation of bacteria using semi-spherical drops
Cryobiology. 2021 Nov 25:S0011-2240(21)00354-0. doi: 10.1016/j.cryobiol.2021.11.179. Online ahead of print.ABSTRACTCryopreservation is a widely used long-term preservation method to ensure the quality and vitality of microbes in laboratories and biological resource facilities. However, freeze-thaw damage and osmotic pressure changes during cryopreservation adversely impacts microbial survival. Significant expenditure of resources and expertise are required to select the right cryoprotectant and optimize its concentration for maximum survival of diverse microorganisms. This work describes a cryopreservation method that obvi...
Source: Cryobiology - November 28, 2021 Category: Biology Authors: Jian Wern Ong Zhixiong Song Hassan Ali Abid Eric Shen Lin Oi Wah Liew Tuck Wah Ng Source Type: research

Cryoprotectant-free preservation of bacteria using semi-spherical drops
Cryobiology. 2021 Nov 25:S0011-2240(21)00354-0. doi: 10.1016/j.cryobiol.2021.11.179. Online ahead of print.ABSTRACTCryopreservation is a widely used long-term preservation method to ensure the quality and vitality of microbes in laboratories and biological resource facilities. However, freeze-thaw damage and osmotic pressure changes during cryopreservation adversely impacts microbial survival. Significant expenditure of resources and expertise are required to select the right cryoprotectant and optimize its concentration for maximum survival of diverse microorganisms. This work describes a cryopreservation method that obvi...
Source: Cryobiology - November 28, 2021 Category: Biology Authors: Jian Wern Ong Zhixiong Song Hassan Ali Abid Eric Shen Lin Oi Wah Liew Tuck Wah Ng Source Type: research

Ice structuring protein extract of Hordeum vulgare var. dolma grains reduces drip loss and loss of soluble vitamin content in peas during frozen storage
Cryobiology. 2021 Nov 23:S0011-2240(21)00353-9. doi: 10.1016/j.cryobiol.2021.11.178. Online ahead of print.ABSTRACTThe Himalayan plants face low temperature during their life cycle and are expected to possess antifreeze proteins. The present work describes screening of six plant species that grow in Himalayas, for their ice structuring properties with an aim to provide a vegetarian source of ice structuring proteins. The ice-structuring protein extract of barley, Hordeum vulgare; variety Dolma (HvISE) and wheat, Triticum aestivum; variety Him Pratham DH 114 (TaISE) showed inhibition of growth of ice crystals during the pro...
Source: Cryobiology - November 26, 2021 Category: Biology Authors: Prakriti Kashyap Sanjay Kumar Source Type: research

Ice structuring protein extract of Hordeum vulgare var. dolma grains reduces drip loss and loss of soluble vitamin content in peas during frozen storage
Cryobiology. 2021 Nov 23:S0011-2240(21)00353-9. doi: 10.1016/j.cryobiol.2021.11.178. Online ahead of print.ABSTRACTThe Himalayan plants face low temperature during their life cycle and are expected to possess antifreeze proteins. The present work describes screening of six plant species that grow in Himalayas, for their ice structuring properties with an aim to provide a vegetarian source of ice structuring proteins. The ice-structuring protein extract of barley, Hordeum vulgare; variety Dolma (HvISE) and wheat, Triticum aestivum; variety Him Pratham DH 114 (TaISE) showed inhibition of growth of ice crystals during the pro...
Source: Cryobiology - November 26, 2021 Category: Biology Authors: Prakriti Kashyap Sanjay Kumar Source Type: research

Ice structuring protein extract of Hordeum vulgare var. dolma grains reduces drip loss and loss of soluble vitamin content in peas during frozen storage
Cryobiology. 2021 Nov 23:S0011-2240(21)00353-9. doi: 10.1016/j.cryobiol.2021.11.178. Online ahead of print.ABSTRACTThe Himalayan plants face low temperature during their life cycle and are expected to possess antifreeze proteins. The present work describes screening of six plant species that grow in Himalayas, for their ice structuring properties with an aim to provide a vegetarian source of ice structuring proteins. The ice-structuring protein extract of barley, Hordeum vulgare; variety Dolma (HvISE) and wheat, Triticum aestivum; variety Him Pratham DH 114 (TaISE) showed inhibition of growth of ice crystals during the pro...
Source: Cryobiology - November 26, 2021 Category: Biology Authors: Prakriti Kashyap Sanjay Kumar Source Type: research

Single sperm vitrification with permeable cryoprotectant-free medium is more effective in patients with severe oligozoospermia and azoospermia
Cryobiology. 2021 Nov 22:S0011-2240(21)00351-5. doi: 10.1016/j.cryobiol.2021.11.176. Online ahead of print.ABSTRACTTesticular sperm extraction (TESE) is an invasive surgery for achieving the spermatozoa in cases with azoospermia. In these patients, the number of retrieved spermatozoa is limited and the optimal cryo-storage is very critical for their fertility preservation. Therefore, single sperm vitrification has been introduced for preservation of low number of spermatozoa. The goal was to assess the efficacy of sperm freezing medium (SFM) and sucrose medium as cryoprotectants for single sperm vitrification in cases with...
Source: Cryobiology - November 25, 2021 Category: Biology Authors: Behnam Maleki Mohammad Ali Khalili Lida Gholizadeh Esmat Mangoli Azam Agha-Rahimi Source Type: research

Solid surface vitrification of goat testicular cell suspension enriched for spermatogonial stem cells
This study reports solid surface vitrification (SSV) of goat testicular cell suspensions (TCS) enriched for spermatogonial stem cells (SSCs). The TCS was isolated from pre-pubertal goat testis by enzymatic digestion, enriched for SSCs by filtration and differential plating, and were vitrified-warmed by SSV. The study showed that SSV could successfully vitrify goat TCS although the percentage of live cells in the vitrified-warmed group was lower (74.8 ± 4.1%) than in non-vitrified control (80.6 ± 6.27%). The vitrified-warmed TCS formed putative SSC colonies upon their in vitro culture, but the colony size of v...
Source: Cryobiology - November 25, 2021 Category: Biology Authors: Tanushree Patra Mukesh Kumar Gupta Source Type: research

Study on cryopreservation of mouse single seminiferous tubule
This study proposes several improved strategies for cryopreservation of single seminiferous tubule in mice. First, single seminiferous tubule was cryopreserved with modified slow freezing and vitrification methods. The results showed that the apoptosis negative rates of spermatogenic cells in single seminiferous tubule with modified slow freezing method were significantly higher than vitrification with plastic slide. Then, plastic slide and two metal vitrification carriers with high thermal conductivity, copper mesh and aluminum foil, were used to vitrify single seminiferous tubule. The metal carriers could improve the out...
Source: Cryobiology - November 23, 2021 Category: Biology Authors: Shuyong Zhang Yingying Guo Liangyu Zhao Sha Han Zheng Li Xinli Zhou Source Type: research

Study on cryopreservation of mouse single seminiferous tubule
This study proposes several improved strategies for cryopreservation of single seminiferous tubule in mice. First, single seminiferous tubule was cryopreserved with modified slow freezing and vitrification methods. The results showed that the apoptosis negative rates of spermatogenic cells in single seminiferous tubule with modified slow freezing method were significantly higher than vitrification with plastic slide. Then, plastic slide and two metal vitrification carriers with high thermal conductivity, copper mesh and aluminum foil, were used to vitrify single seminiferous tubule. The metal carriers could improve the out...
Source: Cryobiology - November 23, 2021 Category: Biology Authors: Shuyong Zhang Yingying Guo Liangyu Zhao Sha Han Zheng Li Xinli Zhou Source Type: research

Study on cryopreservation of mouse single seminiferous tubule
This study proposes several improved strategies for cryopreservation of single seminiferous tubule in mice. First, single seminiferous tubule was cryopreserved with modified slow freezing and vitrification methods. The results showed that the apoptosis negative rates of spermatogenic cells in single seminiferous tubule with modified slow freezing method were significantly higher than vitrification with plastic slide. Then, plastic slide and two metal vitrification carriers with high thermal conductivity, copper mesh and aluminum foil, were used to vitrify single seminiferous tubule. The metal carriers could improve the out...
Source: Cryobiology - November 23, 2021 Category: Biology Authors: Shuyong Zhang Yingying Guo Liangyu Zhao Sha Han Zheng Li Xinli Zhou Source Type: research

Proteomic profiling of cryopreserved Trichormus variabilis using various cryoprotectants
Cryobiology. 2021 Nov 19:S0011-2240(21)00350-3. doi: 10.1016/j.cryobiol.2021.11.175. Online ahead of print.ABSTRACTAlgae, which may be unicellular or multicellular, can carry out photosynthesis just like plants as they effectively utilize light energy. They contain various physiologically active substances and are, therefore, widely used commercially to produce healthy food and feed additives, cosmetics, and energy supplements. For useful applications, the cryopreservation technique has been used in various fields. Recently, to develop suitable cryopreservation methods for algal applications, various studies have been perf...
Source: Cryobiology - November 22, 2021 Category: Biology Authors: Jeong-Won Bae Mirye Park Chang Soo Lee Woo-Sung Kwon Source Type: research