A pilot study of spray cryotherapy effects on airway secretions
Cryobiology. 2021 Jul 23:S0011-2240(21)00123-1. doi: 10.1016/j.cryobiol.2021.07.010. Online ahead of print.ABSTRACTSpray cryotherapy (SCT) is a new transbronchial approach that disrupts epithelial cells by freezing. However, there are limited data addressing the effect of SCT on airway secretion. The aim of this study was to evaluate if SCT effect on airway secretion and the possible mechanism in canines. Fifteen labradors were randomly scheduled SCT or sham operation. Six labradors were scheduled SCT for a short-time observation, and six for a long-time observation, the remaining three received sham operation as control. ...
Source: Cryobiology - July 26, 2021 Category: Biology Authors: Hongxia Duan Xuan Li Xuan Long Xinyang Liu Changhui Wang Shuanshuan Xie Source Type: research

A pilot study of spray cryotherapy effects on airway secretions
Cryobiology. 2021 Jul 23:S0011-2240(21)00123-1. doi: 10.1016/j.cryobiol.2021.07.010. Online ahead of print.ABSTRACTSpray cryotherapy (SCT) is a new transbronchial approach that disrupts epithelial cells by freezing. However, there are limited data addressing the effect of SCT on airway secretion. The aim of this study was to evaluate if SCT effect on airway secretion and the possible mechanism in canines. Fifteen labradors were randomly scheduled SCT or sham operation. Six labradors were scheduled SCT for a short-time observation, and six for a long-time observation, the remaining three received sham operation as control. ...
Source: Cryobiology - July 26, 2021 Category: Biology Authors: Hongxia Duan Xuan Li Xuan Long Xinyang Liu Changhui Wang Shuanshuan Xie Source Type: research

The clinical efficacy of argon-helium knife cryoablation combined with nivolumab in the treatment of advanced non-small cell lung cancer
Cryobiology. 2021 Jul 21:S0011-2240(21)00120-6. doi: 10.1016/j.cryobiol.2021.07.007. Online ahead of print.ABSTRACTTo investigate the clinical safety and efficacy of argon-helium knife cryoablation combined with nivolumab in the treatment of advanced non-small cell lung cancer (NSCLC), 64 patients with advanced NSCLC were retrospectively reviewed. From July 2019 to December 2019, all patients received argon-helium knife cryoablation combined with nivolumab (cryo-nivolumab group, n = 32) or cryoablation alone (cryoablation group, n = 32) at Guangzhou Fuda Cancer Hospital. Short-term efficacy, adverse effects, immune functio...
Source: Cryobiology - July 24, 2021 Category: Biology Authors: Jiang Feng Dong Guiyu Wang Xiongwen Source Type: research

First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media
This study aims to compare the concentration of ProAKAP4 in the semen from 5 stallions frozen with two different extenders immediately after thawing (T0) and 4 hours post-thawing (T4). Viability, total and progressive motility were measured in parallel. Significant differences for sperm viability and total motility were observed between the two extenders, as was the concentration of ProAKAP4 both at T0 and T4. At T4, all quality parameters and proAKAP4 content significantly decreased compared to T0, but with a considerably slower decrease in one extender than the other. These preliminary results suggest that measuring the ...
Source: Cryobiology - July 24, 2021 Category: Biology Authors: D Blommaert N Sergeant M Delehedde I Donnay J P Lejeune T Franck D Serteyn Source Type: research

The clinical efficacy of argon-helium knife cryoablation combined with nivolumab in the treatment of advanced non-small cell lung cancer
Cryobiology. 2021 Jul 21:S0011-2240(21)00120-6. doi: 10.1016/j.cryobiol.2021.07.007. Online ahead of print.ABSTRACTTo investigate the clinical safety and efficacy of argon-helium knife cryoablation combined with nivolumab in the treatment of advanced non-small cell lung cancer (NSCLC), 64 patients with advanced NSCLC were retrospectively reviewed. From July 2019 to December 2019, all patients received argon-helium knife cryoablation combined with nivolumab (cryo-nivolumab group, n = 32) or cryoablation alone (cryoablation group, n = 32) at Guangzhou Fuda Cancer Hospital. Short-term efficacy, adverse effects, immune functio...
Source: Cryobiology - July 24, 2021 Category: Biology Authors: Jiang Feng Dong Guiyu Wang Xiongwen Source Type: research

First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media
This study aims to compare the concentration of ProAKAP4 in the semen from 5 stallions frozen with two different extenders immediately after thawing (T0) and 4 hours post-thawing (T4). Viability, total and progressive motility were measured in parallel. Significant differences for sperm viability and total motility were observed between the two extenders, as was the concentration of ProAKAP4 both at T0 and T4. At T4, all quality parameters and proAKAP4 content significantly decreased compared to T0, but with a considerably slower decrease in one extender than the other. These preliminary results suggest that measuring the ...
Source: Cryobiology - July 24, 2021 Category: Biology Authors: D Blommaert N Sergeant M Delehedde I Donnay J P Lejeune T Franck D Serteyn Source Type: research

Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse GV Oocytes Potentially by Modulating MAD2 Protein Expression of SAC Component Through MTRs
This study was conducted to investigate whether MT can improve in vitro developmental potential of vitrified-warmed GV oocytes through its receptors. The fresh oocytes were randomly divided into four groups: untreated (control group, F), vitrified by open-pulled straw method (vitrification group, V), vitrification group with 100 nmol/L MT supplementation (vitrification + MT group, VM), and with 100 nmol/L MT plus 100 nmol/L luzindole administration (vitrification + MT + luzindole group, VML) or with 50 nmol/L ramelteon addition (vitrification + ramelteon group; VR). After warming, oocytes were cultured in vitro, and MT rec...
Source: Cryobiology - July 23, 2021 Category: Biology Authors: Jinyu Yang Shichao Guo Bo Pan Izhar Hyder Qazi Jianpeng Qin Shengqin Zang Hongbing Han Qingyong Meng Guangbin Zhou Source Type: research

Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse GV Oocytes Potentially by Modulating MAD2 Protein Expression of SAC Component Through MTRs
This study was conducted to investigate whether MT can improve in vitro developmental potential of vitrified-warmed GV oocytes through its receptors. The fresh oocytes were randomly divided into four groups: untreated (control group, F), vitrified by open-pulled straw method (vitrification group, V), vitrification group with 100 nmol/L MT supplementation (vitrification + MT group, VM), and with 100 nmol/L MT plus 100 nmol/L luzindole administration (vitrification + MT + luzindole group, VML) or with 50 nmol/L ramelteon addition (vitrification + ramelteon group; VR). After warming, oocytes were cultured in vitro, and MT rec...
Source: Cryobiology - July 23, 2021 Category: Biology Authors: Jinyu Yang Shichao Guo Bo Pan Izhar Hyder Qazi Jianpeng Qin Shengqin Zang Hongbing Han Qingyong Meng Guangbin Zhou Source Type: research

Stable suppression of skeletal muscle fructose-1,6-bisphosphatase during ground squirrel hibernation: Potential implications of reversible acetylation as a regulatory mechanism
This study increases our understanding of skeletal muscle carbohydrate metabolism during mammalian hibernation and sets forth a potentially novel mechanism for the regulation of FBPase during environmental stress.PMID:34274341 | DOI:10.1016/j.cryobiol.2021.07.006 (Source: Cryobiology)
Source: Cryobiology - July 18, 2021 Category: Biology Authors: Ryan A V Bell None Kenneth B Storey Source Type: research

Stable suppression of skeletal muscle fructose-1,6-bisphosphatase during ground squirrel hibernation: Potential implications of reversible acetylation as a regulatory mechanism
This study increases our understanding of skeletal muscle carbohydrate metabolism during mammalian hibernation and sets forth a potentially novel mechanism for the regulation of FBPase during environmental stress.PMID:34274341 | DOI:10.1016/j.cryobiol.2021.07.006 (Source: Cryobiology)
Source: Cryobiology - July 18, 2021 Category: Biology Authors: Ryan A V Bell None Kenneth B Storey Source Type: research

Stable suppression of skeletal muscle fructose-1,6-bisphosphatase during ground squirrel hibernation: Potential implications of reversible acetylation as a regulatory mechanism
This study increases our understanding of skeletal muscle carbohydrate metabolism during mammalian hibernation and sets forth a potentially novel mechanism for the regulation of FBPase during environmental stress.PMID:34274341 | DOI:10.1016/j.cryobiol.2021.07.006 (Source: Cryobiology)
Source: Cryobiology - July 18, 2021 Category: Biology Authors: Ryan A V Bell None Kenneth B Storey Source Type: research

Stable suppression of skeletal muscle fructose-1,6-bisphosphatase during ground squirrel hibernation: Potential implications of reversible acetylation as a regulatory mechanism
This study increases our understanding of skeletal muscle carbohydrate metabolism during mammalian hibernation and sets forth a potentially novel mechanism for the regulation of FBPase during environmental stress.PMID:34274341 | DOI:10.1016/j.cryobiol.2021.07.006 (Source: Cryobiology)
Source: Cryobiology - July 18, 2021 Category: Biology Authors: Ryan A V Bell None Kenneth B Storey Source Type: research

Aloe vera increases mRNA expression of antioxidant enzymes in cryopreserved bovine ovarian tissue and promotes follicular growth and survival after in vitro culture
Cryobiology. 2021 Jul 13:S0011-2240(21)00118-8. doi: 10.1016/j.cryobiol.2021.07.005. Online ahead of print.ABSTRACTThe aims of the present study were to evaluate the effects of Aloe vera extract on expression of mRNA for antioxidant enzymes in bovine ovarian tissue after vitrification, as well as on follicular morphology, viability, activation and extracellular matrix in cultured ovarian tissues that had been previously vitrified. Fragments from bovine ovarian cortical tissue were cryopreserved in a vitrification solution alone or supplemented with two concentrations of Aloe vera (10 or 50%). After thawing, the cryopreserv...
Source: Cryobiology - July 16, 2021 Category: Biology Authors: Francisco das Chagas Costa Erl ândia Márcia Vasconcelos Ven ância Antônia Nunes Azevedo La ís Raiane Feitosa Melo Paulino M ônica Dias Soares Jos é Roberto Viana Silva Anderson Weiny Barbalho Silva Ana Liza Paz Souza Source Type: research

Influence of helium, xenon, and other noble gases on cryopreservation of Hela and l929 cell lines
Cryobiology. 2021 Jul 13:S0011-2240(21)00117-6. doi: 10.1016/j.cryobiol.2021.07.004. Online ahead of print.ABSTRACTAny biological material contains dissolved gases that affect physical and biological processes associated with cooling and freezing. However, in the cryobiology literature, little attention has been paid to the effect of gasses on cryopreservation. We studied the influence of helium, neon, krypton, xenon, argon, nitrogen, and sulfur hexafluoride on the survivability of HeLa and L929 cell lines during cryopreservation. Saturation of a cell suspension with helium, neon, and sulfur hexafluoride enhanced survival ...
Source: Cryobiology - July 16, 2021 Category: Biology Authors: Natalia V Shishova Svetlana A Ugraitskaya Nikolay E Shvirst Svetlana A Kaurova Eugeny L Gagarinsky Anatoliy L Kovtun Eugeny E Fesenko Source Type: research

Aloe vera increases mRNA expression of antioxidant enzymes in cryopreserved bovine ovarian tissue and promotes follicular growth and survival after in vitro culture
Cryobiology. 2021 Jul 13:S0011-2240(21)00118-8. doi: 10.1016/j.cryobiol.2021.07.005. Online ahead of print.ABSTRACTThe aims of the present study were to evaluate the effects of Aloe vera extract on expression of mRNA for antioxidant enzymes in bovine ovarian tissue after vitrification, as well as on follicular morphology, viability, activation and extracellular matrix in cultured ovarian tissues that had been previously vitrified. Fragments from bovine ovarian cortical tissue were cryopreserved in a vitrification solution alone or supplemented with two concentrations of Aloe vera (10 or 50%). After thawing, the cryopreserv...
Source: Cryobiology - July 16, 2021 Category: Biology Authors: Francisco das Chagas Costa Erl ândia Márcia Vasconcelos Ven ância Antônia Nunes Azevedo La ís Raiane Feitosa Melo Paulino M ônica Dias Soares Jos é Roberto Viana Silva Anderson Weiny Barbalho Silva Ana Liza Paz Souza Source Type: research

Influence of helium, xenon, and other noble gases on cryopreservation of Hela and l929 cell lines
Cryobiology. 2021 Jul 13:S0011-2240(21)00117-6. doi: 10.1016/j.cryobiol.2021.07.004. Online ahead of print.ABSTRACTAny biological material contains dissolved gases that affect physical and biological processes associated with cooling and freezing. However, in the cryobiology literature, little attention has been paid to the effect of gasses on cryopreservation. We studied the influence of helium, neon, krypton, xenon, argon, nitrogen, and sulfur hexafluoride on the survivability of HeLa and L929 cell lines during cryopreservation. Saturation of a cell suspension with helium, neon, and sulfur hexafluoride enhanced survival ...
Source: Cryobiology - July 16, 2021 Category: Biology Authors: Natalia V Shishova Svetlana A Ugraitskaya Nikolay E Shvirst Svetlana A Kaurova Eugeny L Gagarinsky Anatoliy L Kovtun Eugeny E Fesenko Source Type: research

Aloe vera increases mRNA expression of antioxidant enzymes in cryopreserved bovine ovarian tissue and promotes follicular growth and survival after in vitro culture
Cryobiology. 2021 Jul 13:S0011-2240(21)00118-8. doi: 10.1016/j.cryobiol.2021.07.005. Online ahead of print.ABSTRACTThe aims of the present study were to evaluate the effects of Aloe vera extract on expression of mRNA for antioxidant enzymes in bovine ovarian tissue after vitrification, as well as on follicular morphology, viability, activation and extracellular matrix in cultured ovarian tissues that had been previously vitrified. Fragments from bovine ovarian cortical tissue were cryopreserved in a vitrification solution alone or supplemented with two concentrations of Aloe vera (10 or 50%). After thawing, the cryopreserv...
Source: Cryobiology - July 16, 2021 Category: Biology Authors: Francisco das Chagas Costa Erl ândia Márcia Vasconcelos Ven ância Antônia Nunes Azevedo La ís Raiane Feitosa Melo Paulino M ônica Dias Soares Jos é Roberto Viana Silva Anderson Weiny Barbalho Silva Ana Liza Paz Souza Source Type: research

Influence of helium, xenon, and other noble gases on cryopreservation of Hela and l929 cell lines
Cryobiology. 2021 Jul 13:S0011-2240(21)00117-6. doi: 10.1016/j.cryobiol.2021.07.004. Online ahead of print.ABSTRACTAny biological material contains dissolved gases that affect physical and biological processes associated with cooling and freezing. However, in the cryobiology literature, little attention has been paid to the effect of gasses on cryopreservation. We studied the influence of helium, neon, krypton, xenon, argon, nitrogen, and sulfur hexafluoride on the survivability of HeLa and L929 cell lines during cryopreservation. Saturation of a cell suspension with helium, neon, and sulfur hexafluoride enhanced survival ...
Source: Cryobiology - July 16, 2021 Category: Biology Authors: Natalia V Shishova Svetlana A Ugraitskaya Nikolay E Shvirst Svetlana A Kaurova Eugeny L Gagarinsky Anatoliy L Kovtun Eugeny E Fesenko Source Type: research

The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in Turkey semen during liquid storage at 5  °C
Cryobiology. 2021 Jul 8:S0011-2240(21)00115-2. doi: 10.1016/j.cryobiol.2021.07.002. Online ahead of print.ABSTRACTSince turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 °C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. A glucose-based extender was used to dilute the pooled semen. It wa...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Hasan Izanloo Ali Soleimanzadeh Mustafa Numan Bucak Mehdi Imani Mahdi Zhandi Source Type: research

Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm
Cryobiology. 2021 Jul 7:S0011-2240(21)00116-4. doi: 10.1016/j.cryobiol.2021.07.003. Online ahead of print.ABSTRACTContamination of fish milt during collection can have an important effect on the quality of fresh and frozen samples. The aim of this study was to evaluate the effects of biological contaminants (urine, feces, and blood) on the sperm of Colossoma macropomum. After hormonal induction, contaminated and contaminant-free milt samples from thirteen males (6.48 ± 2.82 kg) were collected and frozen. The sperm motility was evaluated in fresh and frozen-thawed sperm. Membrane and DNA integrity and mitochondrial f...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Lis Santos Marques Luis Fernando Guerrero Gracia Maritza P érez Atehortúa R ômulo Batista Rodrigues Nathalia Dos Santos Teixeira Eneder Rosana Oberst Danilo Pedro Streit Source Type: research

The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in Turkey semen during liquid storage at 5  °C
Cryobiology. 2021 Jul 8:S0011-2240(21)00115-2. doi: 10.1016/j.cryobiol.2021.07.002. Online ahead of print.ABSTRACTSince turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 °C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. A glucose-based extender was used to dilute the pooled semen. It wa...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Hasan Izanloo Ali Soleimanzadeh Mustafa Numan Bucak Mehdi Imani Mahdi Zhandi Source Type: research

Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm
Cryobiology. 2021 Jul 7:S0011-2240(21)00116-4. doi: 10.1016/j.cryobiol.2021.07.003. Online ahead of print.ABSTRACTContamination of fish milt during collection can have an important effect on the quality of fresh and frozen samples. The aim of this study was to evaluate the effects of biological contaminants (urine, feces, and blood) on the sperm of Colossoma macropomum. After hormonal induction, contaminated and contaminant-free milt samples from thirteen males (6.48 ± 2.82 kg) were collected and frozen. The sperm motility was evaluated in fresh and frozen-thawed sperm. Membrane and DNA integrity and mitochondrial f...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Lis Santos Marques Luis Fernando Guerrero Gracia Maritza P érez Atehortúa R ômulo Batista Rodrigues Nathalia Dos Santos Teixeira Eneder Rosana Oberst Danilo Pedro Streit Source Type: research

The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in Turkey semen during liquid storage at 5  °C
Cryobiology. 2021 Jul 8:S0011-2240(21)00115-2. doi: 10.1016/j.cryobiol.2021.07.002. Online ahead of print.ABSTRACTSince turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 °C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. A glucose-based extender was used to dilute the pooled semen. It wa...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Hasan Izanloo Ali Soleimanzadeh Mustafa Numan Bucak Mehdi Imani Mahdi Zhandi Source Type: research

Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm
Cryobiology. 2021 Jul 7:S0011-2240(21)00116-4. doi: 10.1016/j.cryobiol.2021.07.003. Online ahead of print.ABSTRACTContamination of fish milt during collection can have an important effect on the quality of fresh and frozen samples. The aim of this study was to evaluate the effects of biological contaminants (urine, feces, and blood) on the sperm of Colossoma macropomum. After hormonal induction, contaminated and contaminant-free milt samples from thirteen males (6.48 ± 2.82 kg) were collected and frozen. The sperm motility was evaluated in fresh and frozen-thawed sperm. Membrane and DNA integrity and mitochondrial f...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Lis Santos Marques Luis Fernando Guerrero Gracia Maritza P érez Atehortúa R ômulo Batista Rodrigues Nathalia Dos Santos Teixeira Eneder Rosana Oberst Danilo Pedro Streit Source Type: research

The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in turkey semen during liquid storage at 5 °C
Cryobiology. 2021 Jul 7:S0011-2240(21)00115-2. doi: 10.1016/j.cryobiol.2021.07.002. Online ahead of print.ABSTRACTSince turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 °C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. A glucose-based extender was used to dilute the pooled semen. It wa...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Hasan Izanloo Ali Soleimanzadeh Mustafa Numan Bucak Mehdi Imani Mahdi Zhandi Source Type: research

Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm
Cryobiology. 2021 Jul 7:S0011-2240(21)00116-4. doi: 10.1016/j.cryobiol.2021.07.003. Online ahead of print.ABSTRACTContamination of fish milt during collection can have an important effect on the quality of fresh and frozen samples. The aim of this study was to evaluate the effects of biological contaminants (urine, feces, and blood) on the sperm of Colossoma macropomum. After hormonal induction, contaminated and contaminant-free milt samples from thirteen males (6.48 ± 2.82 kg) were collected and frozen. The sperm motility was evaluated in fresh and frozen-thawed sperm. Membrane and DNA integrity and mitochondrial f...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Lis Santos Marques Luis Fernando Guerrero Gracia Maritza P érez Atehortúa R ômulo Batista Rodrigues Nathalia Dos Santos Teixeira Eneder Rosana Oberst Danilo Pedro Streit Source Type: research

The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in turkey semen during liquid storage at 5 °C
Cryobiology. 2021 Jul 7:S0011-2240(21)00115-2. doi: 10.1016/j.cryobiol.2021.07.002. Online ahead of print.ABSTRACTSince turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 °C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. A glucose-based extender was used to dilute the pooled semen. It wa...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Hasan Izanloo Ali Soleimanzadeh Mustafa Numan Bucak Mehdi Imani Mahdi Zhandi Source Type: research

Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm
Cryobiology. 2021 Jul 7:S0011-2240(21)00116-4. doi: 10.1016/j.cryobiol.2021.07.003. Online ahead of print.ABSTRACTContamination of fish milt during collection can have an important effect on the quality of fresh and frozen samples. The aim of this study was to evaluate the effects of biological contaminants (urine, feces, and blood) on the sperm of Colossoma macropomum. After hormonal induction, contaminated and contaminant-free milt samples from thirteen males (6.48 ± 2.82 kg) were collected and frozen. The sperm motility was evaluated in fresh and frozen-thawed sperm. Membrane and DNA integrity and mitochondrial f...
Source: Cryobiology - July 10, 2021 Category: Biology Authors: Lis Santos Marques Luis Fernando Guerrero Gracia Maritza P érez Atehortúa R ômulo Batista Rodrigues Nathalia Dos Santos Teixeira Eneder Rosana Oberst Danilo Pedro Streit Source Type: research

Effect of Different Carboxylated Poly L-lysine and Dimethyl Sulfoxide Combinations on Post Thaw Rabbit Sperm Functionality and Fertility
Cryobiology. 2021 Jul 6:S0011-2240(21)00114-0. doi: 10.1016/j.cryobiol.2021.07.001. Online ahead of print.ABSTRACTAntifreeze proteins are biologically active substances which protect living organisms against freezing injuries. The effect of a synthetic antifreeze protein carboxylated poly l-lysine (CPLL) in the extender was evaluated in the presence of a conventional cryoprotective agent, dimethyl sulfoxide (Me2SO), for freezing rabbit sperm cells. The experiment was conducted according to 2x3 factorial design including two Me2SO (5 or 8%) and three CPLL (0, 0.5 or 1%) concentrations. CPLL supplementation improved live and...
Source: Cryobiology - July 9, 2021 Category: Biology Authors: Niyazi K üçük Sanan Raza Kazuaki Matsumura U ğur Uçan İlker Serin Ahmet Ceylan Melih Aksoy Source Type: research

Effect of Different Carboxylated Poly L-lysine and Dimethyl Sulfoxide Combinations on Post Thaw Rabbit Sperm Functionality and Fertility
Cryobiology. 2021 Jul 6:S0011-2240(21)00114-0. doi: 10.1016/j.cryobiol.2021.07.001. Online ahead of print.ABSTRACTAntifreeze proteins are biologically active substances which protect living organisms against freezing injuries. The effect of a synthetic antifreeze protein carboxylated poly l-lysine (CPLL) in the extender was evaluated in the presence of a conventional cryoprotective agent, dimethyl sulfoxide (Me2SO), for freezing rabbit sperm cells. The experiment was conducted according to 2x3 factorial design including two Me2SO (5 or 8%) and three CPLL (0, 0.5 or 1%) concentrations. CPLL supplementation improved live and...
Source: Cryobiology - July 9, 2021 Category: Biology Authors: Niyazi K üçük Sanan Raza Kazuaki Matsumura U ğur Uçan İlker Serin Ahmet Ceylan Melih Aksoy Source Type: research

Toxicity of cryoprotective agents to semen from two closely related snake species: The endangered Louisiana pinesnake (Pituophis ruthveni) and bullsnake (Pituophis cantenifer)
Cryobiology. 2021 Jul 2:S0011-2240(21)00113-9. doi: 10.1016/j.cryobiol.2021.06.008. Online ahead of print.ABSTRACTCryopreservation of sperm is an important tool for the conservation of threatened species. Many species of reptile are under considerable threat of extinction and there has been limited investigation of sperm cryopreservation in this taxonomic group. We performed a comparative test of toxicity to sperm of six commonly used cryoprotective agents (CPAs) at three concentrations (5%, 10%, 20%) from the Louisiana pinesnake, Pituophis ruthveni (n = 11), and the closely related bullsnake, Pituophis cantenifer (n = 8)....
Source: Cryobiology - July 5, 2021 Category: Biology Authors: Mark R Sandfoss Oliver M Whittington Steve Reichling Beth M Roberts Source Type: research

Toxicity of cryoprotective agents to semen from two closely related snake species: the endangered Louisiana pinesnake (Pituophis ruthveni) and bullsnake (Pituophis cantenifer)
Cryobiology. 2021 Jul 2:S0011-2240(21)00113-9. doi: 10.1016/j.cryobiol.2021.06.008. Online ahead of print.ABSTRACTCryopreservation of sperm is an important tool for the conservation of threatened species. Many species of reptile are under considerable threat of extinction and there has been limited investigation of sperm cryopreservation in this taxonomic group. We performed a comparative test of toxicity to sperm of six commonly used cryoprotective agents (CPAs) at three concentrations (5%, 10%, 20%) from the Louisiana pinesnake, Pituophis ruthveni (n = 11), and the closely related bullsnake, Pituophis cantenifer (n = 8)....
Source: Cryobiology - July 5, 2021 Category: Biology Authors: Mark R Sandfoss Oliver M Whittington Steve Reichling Beth M Roberts Source Type: research

Toxicity of cryoprotective agents to semen from two closely related snake species: the endangered Louisiana pinesnake (Pituophis ruthveni) and bullsnake (Pituophis cantenifer)
Cryobiology. 2021 Jul 2:S0011-2240(21)00113-9. doi: 10.1016/j.cryobiol.2021.06.008. Online ahead of print.ABSTRACTCryopreservation of sperm is an important tool for the conservation of threatened species. Many species of reptile are under considerable threat of extinction and there has been limited investigation of sperm cryopreservation in this taxonomic group. We performed a comparative test of toxicity to sperm of six commonly used cryoprotective agents (CPAs) at three concentrations (5%, 10%, 20%) from the Louisiana pinesnake, Pituophis ruthveni (n = 11), and the closely related bullsnake, Pituophis cantenifer (n = 8)....
Source: Cryobiology - July 5, 2021 Category: Biology Authors: Mark R Sandfoss Oliver M Whittington Steve Reichling Beth M Roberts Source Type: research

Toxicity of cryoprotective agents to semen from two closely related snake species: the endangered Louisiana pinesnake (Pituophis ruthveni) and bullsnake (Pituophis cantenifer)
Cryobiology. 2021 Jul 2:S0011-2240(21)00113-9. doi: 10.1016/j.cryobiol.2021.06.008. Online ahead of print.ABSTRACTCryopreservation of sperm is an important tool for the conservation of threatened species. Many species of reptile are under considerable threat of extinction and there has been limited investigation of sperm cryopreservation in this taxonomic group. We performed a comparative test of toxicity to sperm of six commonly used cryoprotective agents (CPAs) at three concentrations (5%, 10%, 20%) from the Louisiana pinesnake, Pituophis ruthveni (n = 11), and the closely related bullsnake, Pituophis cantenifer (n = 8)....
Source: Cryobiology - July 5, 2021 Category: Biology Authors: Mark R Sandfoss Oliver M Whittington Steve Reichling Beth M Roberts Source Type: research

Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation
This study investigated the regulation of citrate synthase in the muscle tissue of a small mammalian hibernator through comparison of functional and structural properties. The results demonstrated a significant decrease in the Vmax of purified torpid CS compared to the control euthermic enzyme (1.2-1.7 fold greater in the control) that was evident over a wide range of temperatures (8, 22 and 37 °C) that are encountered by the enzyme in hibernation. This was also reflected in the specific activity of the enzyme in crude muscle protein extracts. Analyzing the purified CS through immunoblotting demonstrated that the enzym...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Stuart R Green Kenneth B Storey Source Type: research

Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis
Cryobiology. 2021 Jun 26:S0011-2240(21)00112-7. doi: 10.1016/j.cryobiol.2021.06.007. Online ahead of print.ABSTRACTObjectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3473) survival of Bos taurus embryos. The literature was systematically reviewed and data from 40 manuscripts including 78 experiments, and comprising 183 treatment means, were used for the analyses. The in-vitro parameters included rates of re-expansion, hatching, and survival of blastocysts either at 24 h or 72 h post-thawing/warming and to...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Usman Arshad Masroor Sagheer F átima B González-Silvestry Mubbashar Hassan Froylan Sosa Source Type: research

Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation
This study investigated the regulation of citrate synthase in the muscle tissue of a small mammalian hibernator through comparison of functional and structural properties. The results demonstrated a significant decrease in the Vmax of purified torpid CS compared to the control euthermic enzyme (1.2-1.7 fold greater in the control) that was evident over a wide range of temperatures (8, 22 and 37 °C) that are encountered by the enzyme in hibernation. This was also reflected in the specific activity of the enzyme in crude muscle protein extracts. Analyzing the purified CS through immunoblotting demonstrated that the enzym...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Stuart R Green Kenneth B Storey Source Type: research

Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis
Cryobiology. 2021 Jun 26:S0011-2240(21)00112-7. doi: 10.1016/j.cryobiol.2021.06.007. Online ahead of print.ABSTRACTObjectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3,473) survival of Bos taurus embryos. The literature was systematically reviewed and data from 40 manuscripts including 78 experiments, and comprising 183 treatment means, were used for the analyses. The in-vitro parameters included rates of re-expansion, hatching, and survival of blastocysts either at 24 h or 72 h post-thawing/warming and t...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Usman Arshad Masroor Sagheer F átima B González-Silvestry Mubbashar Hassan Froylan Sosa Source Type: research

Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation
This study investigated the regulation of citrate synthase in the muscle tissue of a small mammalian hibernator through comparison of functional and structural properties. The results demonstrated a significant decrease in the Vmax of purified torpid CS compared to the control euthermic enzyme (1.2-1.7 fold greater in the control) that was evident over a wide range of temperatures (8, 22 and 37 °C) that are encountered by the enzyme in hibernation. This was also reflected in the specific activity of the enzyme in crude muscle protein extracts. Analyzing the purified CS through immunoblotting demonstrated that the enzym...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Stuart R Green Kenneth B Storey Source Type: research

Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis
Cryobiology. 2021 Jun 26:S0011-2240(21)00112-7. doi: 10.1016/j.cryobiol.2021.06.007. Online ahead of print.ABSTRACTObjectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3,473) survival of Bos taurus embryos. The literature was systematically reviewed and data from 40 manuscripts including 78 experiments, and comprising 183 treatment means, were used for the analyses. The in-vitro parameters included rates of re-expansion, hatching, and survival of blastocysts either at 24 h or 72 h post-thawing/warming and t...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Usman Arshad Masroor Sagheer F átima B González-Silvestry Mubbashar Hassan Froylan Sosa Source Type: research

Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation
This study investigated the regulation of citrate synthase in the muscle tissue of a small mammalian hibernator through comparison of functional and structural properties. The results demonstrated a significant decrease in the Vmax of purified torpid CS compared to the control euthermic enzyme (1.2-1.7 fold greater in the control) that was evident over a wide range of temperatures (8, 22 and 37 °C) that are encountered by the enzyme in hibernation. This was also reflected in the specific activity of the enzyme in crude muscle protein extracts. Analyzing the purified CS through immunoblotting demonstrated that the enzym...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Stuart R Green Kenneth B Storey Source Type: research

Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis
Cryobiology. 2021 Jun 26:S0011-2240(21)00112-7. doi: 10.1016/j.cryobiol.2021.06.007. Online ahead of print.ABSTRACTObjectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3,473) survival of Bos taurus embryos. The literature was systematically reviewed and data from 40 manuscripts including 78 experiments, and comprising 183 treatment means, were used for the analyses. The in-vitro parameters included rates of re-expansion, hatching, and survival of blastocysts either at 24 h or 72 h post-thawing/warming and t...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Usman Arshad Masroor Sagheer F átima B González-Silvestry Mubbashar Hassan Froylan Sosa Source Type: research

Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation
This study investigated the regulation of citrate synthase in the muscle tissue of a small mammalian hibernator through comparison of functional and structural properties. The results demonstrated a significant decrease in the Vmax of purified torpid CS compared to the control euthermic enzyme (1.2-1.7 fold greater in the control) that was evident over a wide range of temperatures (8, 22 and 37 °C) that are encountered by the enzyme in hibernation. This was also reflected in the specific activity of the enzyme in crude muscle protein extracts. Analyzing the purified CS through immunoblotting demonstrated that the enzym...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Stuart R Green Kenneth B Storey Source Type: research

Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis
Cryobiology. 2021 Jun 26:S0011-2240(21)00112-7. doi: 10.1016/j.cryobiol.2021.06.007. Online ahead of print.ABSTRACTObjectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3,473) survival of Bos taurus embryos. The literature was systematically reviewed and data from 40 manuscripts including 78 experiments, and comprising 183 treatment means, were used for the analyses. The in-vitro parameters included rates of re-expansion, hatching, and survival of blastocysts either at 24 h or 72 h post-thawing/warming and t...
Source: Cryobiology - June 29, 2021 Category: Biology Authors: Usman Arshad Masroor Sagheer F átima B González-Silvestry Mubbashar Hassan Froylan Sosa Source Type: research

Survival of thermophilic fungi in various preservation methods: A comparative study
In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 °C method and lyophilization were better methods for long-term preservation up to 5 years.PMID:34153346 | DOI:10.1016/j.cryobiol.2021.06.005 (Source: Cryobiology)
Source: Cryobiology - June 21, 2021 Category: Biology Authors: Derya Berikten Source Type: research

Survival of thermophilic fungi in various preservation methods: A comparative study
In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 °C method and lyophilization were better methods for long-term preservation up to 5 years.PMID:34153346 | DOI:10.1016/j.cryobiol.2021.06.005 (Source: Cryobiology)
Source: Cryobiology - June 21, 2021 Category: Biology Authors: Derya Berikten Source Type: research

Survival of Thermophilic Fungi in Various Preservation Methods: A Comparative Study
In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 °C method and lyophilization were better methods for long-term preservation up to 5 years.PMID:34153346 | DOI:10.1016/j.cryobiol.2021.06.005 (Source: Cryobiology)
Source: Cryobiology - June 21, 2021 Category: Biology Authors: Derya Berikten Source Type: research

Survival of Thermophilic Fungi in Various Preservation Methods: A Comparative Study
In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 °C method and lyophilization were better methods for long-term preservation up to 5 years.PMID:34153346 | DOI:10.1016/j.cryobiol.2021.06.005 (Source: Cryobiology)
Source: Cryobiology - June 21, 2021 Category: Biology Authors: Derya Berikten Source Type: research

Survival of Thermophilic Fungi in Various Preservation Methods: A Comparative Study
In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 °C method and lyophilization were better methods for long-term preservation up to 5 years.PMID:34153346 | DOI:10.1016/j.cryobiol.2021.06.005 (Source: Cryobiology)
Source: Cryobiology - June 21, 2021 Category: Biology Authors: Derya Berikten Source Type: research

Survival of Thermophilic Fungi in Various Preservation Methods: A Comparative Study
In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 °C method and lyophilization were better methods for long-term preservation up to 5 years.PMID:34153346 | DOI:10.1016/j.cryobiol.2021.06.005 (Source: Cryobiology)
Source: Cryobiology - June 21, 2021 Category: Biology Authors: Derya Berikten Source Type: research