Albumin (ALB) and protein disulfide isomerase family A member 4 (PDIA4) are novel markers to predict sperm freezability of Erhualian boar
In conclusion, these results suggest that ALB and PDIA4 are related to boar sperm cryotolerance and may be used as novel freezability markers.PMID:36181861 | DOI:10.1016/j.cryobiol.2022.09.006 (Source: Cryobiology)
Source: Cryobiology - October 1, 2022 Category: Biology Authors: Shifeng Tong Chang Yin Yuzhu Ge Zhibin Ren Jingli Tao Yang Liu Source Type: research

Media composition to revive leptospires stored at -80  °C
Cryobiology. 2022 Sep 27:S0011-2240(22)00099-2. doi: 10.1016/j.cryobiol.2022.09.007. Online ahead of print.ABSTRACTLeptospires are preserved by frequent sub-culturing in semisolid media due to the challenge of low recovery by freezing or liquid nitrogen methods. The present study evaluated three liquid EMJH medium compositions (Medium A: Leptospira medium base EMJH, Leptospira enrichment EMJH, 5-fluorouracil (3%), rabbit serum (1%) and calf serum (1%); Medium B: same as Medium A but without 5-fluorouracil; Medium C: same as Medium B but with the addition of sodium pyruvate) for the revival of leptospires after storage at -...
Source: Cryobiology - September 30, 2022 Category: Biology Authors: Noraini Philip Vasantha Kumari Neela Source Type: research

Cryopreservation increases accumulation of exogenous stearic acid in mouse embryos
Cryobiology. 2022 Sep 27:S0011-2240(22)00097-9. doi: 10.1016/j.cryobiol.2022.09.005. Online ahead of print.ABSTRACTCryopreservation of preimplantation embryos is a widely used technique, but this procedure might impact the subsequent embryo development. The effect of slow freezing and vitrification on the lipid metabolism in preimplantation mammalian embryos is not well studied. In this work, we applied Raman spectroscopy of isotopically labeled molecules to address the effects of cryopreservation on fatty acid accumulation in mouse embryos. Embryos after slow freezing or vitrification were cultured for 20 h in a medium su...
Source: Cryobiology - September 30, 2022 Category: Biology Authors: A N Omelchenko T N Igonina E Y Brusentsev K A Okotrub S Y Amstislavsky N V Surovtsev Source Type: research

The effect of sucrose supplementation on chondrocyte viability in porcine articular cartilage following vitrification
In conclusion, sucrose-supplemented DMEM + clinical grade CS (Protocol 4) could be an ideal protocol to be investigated for future use in clinical applications involving vitrified AC.PMID:36155184 | DOI:10.1016/j.cryobiol.2022.09.004 (Source: Cryobiology)
Source: Cryobiology - September 26, 2022 Category: Biology Authors: Kar Wey Yong Kezhou Wu JanetA W Elliott Nadr M Jomha Source Type: research

The effect of sucrose supplementation on chondrocyte viability in porcine articular cartilage following vitrification
In conclusion, sucrose-supplemented DMEM + clinical grade CS (Protocol 4) could be an ideal protocol to be investigated for future use in clinical applications involving vitrified AC.PMID:36155184 | DOI:10.1016/j.cryobiol.2022.09.004 (Source: Cryobiology)
Source: Cryobiology - September 26, 2022 Category: Biology Authors: Kar Wey Yong Kezhou Wu JanetA W Elliott Nadr M Jomha Source Type: research

The effect of sucrose supplementation on chondrocyte viability in porcine articular cartilage following vitrification
In conclusion, sucrose-supplemented DMEM + clinical grade CS (Protocol 4) could be an ideal protocol to be investigated for future use in clinical applications involving vitrified AC.PMID:36155184 | DOI:10.1016/j.cryobiol.2022.09.004 (Source: Cryobiology)
Source: Cryobiology - September 26, 2022 Category: Biology Authors: Kar Wey Yong Kezhou Wu JanetA W Elliott Nadr M Jomha Source Type: research

The effect of sucrose supplementation on chondrocyte viability in porcine articular cartilage following vitrification
In conclusion, sucrose-supplemented DMEM + clinical grade CS (Protocol 4) could be an ideal protocol to be investigated for future use in clinical applications involving vitrified AC.PMID:36155184 | DOI:10.1016/j.cryobiol.2022.09.004 (Source: Cryobiology)
Source: Cryobiology - September 26, 2022 Category: Biology Authors: Kar Wey Yong Kezhou Wu JanetA W Elliott Nadr M Jomha Source Type: research

The effect of sucrose supplementation on chondrocyte viability in porcine articular cartilage following vitrification
In conclusion, sucrose-supplemented DMEM + clinical grade CS (Protocol 4) could be an ideal protocol to be investigated for future use in clinical applications involving vitrified AC.PMID:36155184 | DOI:10.1016/j.cryobiol.2022.09.004 (Source: Cryobiology)
Source: Cryobiology - September 26, 2022 Category: Biology Authors: Kar Wey Yong Kezhou Wu JanetA W Elliott Nadr M Jomha Source Type: research

Kinetics of lipid indicators in response to short- and long-duration whole-body, cold-water immersion
Cryobiology. 2022 Sep 20:S0011-2240(22)00095-5. doi: 10.1016/j.cryobiol.2022.09.003. Online ahead of print.ABSTRACTCold exposure-induced secretion of stress hormones activates cold-defense responses and mobilizes substrates for increased energy demands to fuel thermogenesis. However, it is unclear whether acute cold exposure-induced stress hormone response kinetics affect circulating lipid parameter kinetics. Therefore, we aimed to investigate the 2-day kinetics of stress hormones (i.e., cortisol, epinephrine, and norepinephrine) and the lipid profile (i.e., total cholesterol [TC], high-density lipoprotein [HDL] cholestero...
Source: Cryobiology - September 23, 2022 Category: Biology Authors: Milda Eimonte Nerijus Eimantas Neringa Baranauskiene Rima Solianik Marius Brazaitis Source Type: research

Kinetics of lipid indicators in response to short- and long-duration whole-body, cold-water immersion
Cryobiology. 2022 Sep 20:S0011-2240(22)00095-5. doi: 10.1016/j.cryobiol.2022.09.003. Online ahead of print.ABSTRACTCold exposure-induced secretion of stress hormones activates cold-defense responses and mobilizes substrates for increased energy demands to fuel thermogenesis. However, it is unclear whether acute cold exposure-induced stress hormone response kinetics affect circulating lipid parameter kinetics. Therefore, we aimed to investigate the 2-day kinetics of stress hormones (i.e., cortisol, epinephrine, and norepinephrine) and the lipid profile (i.e., total cholesterol [TC], high-density lipoprotein [HDL] cholestero...
Source: Cryobiology - September 23, 2022 Category: Biology Authors: Milda Eimonte Nerijus Eimantas Neringa Baranauskiene Rima Solianik Marius Brazaitis Source Type: research

Comparison of dilute and nondilute osmotic equilibrium models for erythrocytes
Cryobiology. 2022 Sep 18:S0011-2240(22)00093-1. doi: 10.1016/j.cryobiol.2022.09.001. Online ahead of print.ABSTRACTSuccessful cryopreservation requires the addition of cryoprotective agents (CPAs). The addition of permeating CPAs, such as glycerol, is associated with some risk to the cells and tissues. These risks are both related to the CPA themselves (CPA toxicity) and to the volume response of the cell (osmotic damage). To minimize the potential for damage during cryopreservation, mathematical models are often employed to understand the interactions between protocols and cell volume responses. In the literature, this vo...
Source: Cryobiology - September 21, 2022 Category: Biology Authors: R Shuttleworth A Z Higgins A Eroglu J D Benson Source Type: research

Comparison of dilute and nondilute osmotic equilibrium models for erythrocytes
Cryobiology. 2022 Sep 18:S0011-2240(22)00093-1. doi: 10.1016/j.cryobiol.2022.09.001. Online ahead of print.ABSTRACTSuccessful cryopreservation requires the addition of cryoprotective agents (CPAs). The addition of permeating CPAs, such as glycerol, is associated with some risk to the cells and tissues. These risks are both related to the CPA themselves (CPA toxicity) and to the volume response of the cell (osmotic damage). To minimize the potential for damage during cryopreservation, mathematical models are often employed to understand the interactions between protocols and cell volume responses. In the literature, this vo...
Source: Cryobiology - September 21, 2022 Category: Biology Authors: R Shuttleworth A Z Higgins A Eroglu J D Benson Source Type: research

Comparison of dilute and nondilute osmotic equilibrium models for erythrocytes
Cryobiology. 2022 Sep 18:S0011-2240(22)00093-1. doi: 10.1016/j.cryobiol.2022.09.001. Online ahead of print.ABSTRACTSuccessful cryopreservation requires the addition of cryoprotective agents (CPAs). The addition of permeating CPAs, such as glycerol, is associated with some risk to the cells and tissues. These risks are both related to the CPA themselves (CPA toxicity) and to the volume response of the cell (osmotic damage). To minimize the potential for damage during cryopreservation, mathematical models are often employed to understand the interactions between protocols and cell volume responses. In the literature, this vo...
Source: Cryobiology - September 21, 2022 Category: Biology Authors: R Shuttleworth A Z Higgins A Eroglu J D Benson Source Type: research

Pre-stress salicylic-acid treatment as an intervention strategy for freeze-protection in spinach: Foliar versus sub-irrigation application and duration of efficacy
Cryobiology. 2022 Sep 16:S0011-2240(22)00090-6. doi: 10.1016/j.cryobiol.2022.08.004. Online ahead of print.ABSTRACTExogenous application of salicylic acid (SA) to plant tissues has been shown to confer tolerance against various abiotic stresses. Recently, SA application through sub-irrigation was shown to improve plant freezing tolerance (FT). For SA treatment to be employable as an effective intervention strategy for frost protection under field conditions, it is important to study its effect on FT when applied as a foliar spray to whole plants. It is also important to determine for how long the FT-improvement by SA lasts...
Source: Cryobiology - September 19, 2022 Category: Biology Authors: Kyungwon Min Rajeev Arora Source Type: research

Pre-stress salicylic-acid treatment as an intervention strategy for freeze-protection in spinach: Foliar versus sub-irrigation application and duration of efficacy
Cryobiology. 2022 Sep 16:S0011-2240(22)00090-6. doi: 10.1016/j.cryobiol.2022.08.004. Online ahead of print.ABSTRACTExogenous application of salicylic acid (SA) to plant tissues has been shown to confer tolerance against various abiotic stresses. Recently, SA application through sub-irrigation was shown to improve plant freezing tolerance (FT). For SA treatment to be employable as an effective intervention strategy for frost protection under field conditions, it is important to study its effect on FT when applied as a foliar spray to whole plants. It is also important to determine for how long the FT-improvement by SA lasts...
Source: Cryobiology - September 19, 2022 Category: Biology Authors: Kyungwon Min Rajeev Arora Source Type: research