Germplasm of Ozark chinquapin (Castanea ozarkensis Ashe) can be cryopreserved by dormant winter buds
Cryobiology. 2023 Dec 8:104833. doi: 10.1016/j.cryobiol.2023.104833. Online ahead of print.ABSTRACTOzark chinquapin (Castanea ozarkensis Ashe) is a forest tree, endemic to the Ozark Mountain region in Eastern United States. Its nutritious nuts were consumed by Native Americans, European settlers, livestock, and wild animals and its wood was an important rot-resistant construction material. Once a significant tree in regional forest communities, the species was nearly eradicated by a chestnut blight caused by Cryphonectria parasitca (Murill) Barr fungus. Some individuals have survived as sprouts from adventitious root buds,...
Source: Cryobiology - December 10, 2023 Category: Biology Authors: Maria M Jenderek Kathleen M Yeater Andrew L Thomas Source Type: research
Vitrified turkey ovarian tissue cultured and assessed through gene expression patterns: A potential screening tool
Cryobiology. 2023 Dec 8:104837. doi: 10.1016/j.cryobiol.2023.104837. Online ahead of print.ABSTRACTBiobanking of turkey ovarian tissue has the potential to play a crucial part in preserving female genetics. To date, ovarian tissue has only been vitrified using a standard protocol, with immediate analyses after warming, therefore, long-term cryoinjury is unknown. Long-term cryoinjury was investigated here by in-ovo culturing, fresh (non-vitrified), a purposefully suboptimal poor vitrification (PV), and the standard vitrified (StV) protocol. Assessments were performed via cellular morphological changes and mRNA gene expressi...
Source: Cryobiology - December 10, 2023 Category: Biology Authors: George B Hall Amy Shannon Diane Hildenberger Kristen Brady Julie A Long Source Type: research
Development of a Me < sub > 2 < /sub > SO-free cryopreservation medium and its long-term cryoprotection on the CAR-NK cells
In this study, we developed a Me2SO-free cryopreservation medium specifically tailored for CAR-NK cells to address this limitation. The cryopreservation medium was formulated using human serum albumin (HSA) and glycerol as the base components. Following initial screening of seven clinically-compatible solutions, four with cryoprotective properties were identified. These were combined and optimized into a single formulation: IF-M. The viability, phenotype, and function of CAR-NK cells were evaluated after short-term and long-term cryopreservation to assess the effectiveness of IF-M, with Me2SO serving as the control group. ...
Source: Cryobiology - December 9, 2023 Category: Biology Authors: Ruisheng Xu Xiaodi Shi Huimin Huang Wen-Song Tan Haibo Cai Source Type: research
Effects of intracellular Ca < sup > 2+ < /sup > on developmental potential and ultrastructure of cryopreserved-warmed oocyte in mouse
Cryobiology. 2023 Dec 6:104834. doi: 10.1016/j.cryobiol.2023.104834. Online ahead of print.ABSTRACTMaintaining appropriate intracellular calcium of oocytes is necessary to prevent ultrastructure and organelle damage caused by freezing and cryoprotectants. The present study aimed to investigate whether cryoprotectant-induced changes in the calcium concentrations of oocytes can be regulated to reduce damage to developmental potential and ultrastructure. A total of 33 mice and 1381 oocytes were used to explore the effects of intracellular calcium on the development and ultrastructures of oocytes subjected to 2-aminoethoxydiph...
Source: Cryobiology - December 8, 2023 Category: Biology Authors: Junping Cheng Xiaoli Wang Chan Luo Xianbao Mao Jie Qin Yan Chi Bing He Yanrong Hao Xiangli Niu Ben Huang Liling Liu Source Type: research
Effects of intracellular Ca < sup > 2+ < /sup > on developmental potential and ultrastructure of cryopreserved-warmed oocyte in mouse
Cryobiology. 2023 Dec 6:104834. doi: 10.1016/j.cryobiol.2023.104834. Online ahead of print.ABSTRACTMaintaining appropriate intracellular calcium of oocytes is necessary to prevent ultrastructure and organelle damage caused by freezing and cryoprotectants. The present study aimed to investigate whether cryoprotectant-induced changes in the calcium concentrations of oocytes can be regulated to reduce damage to developmental potential and ultrastructure. A total of 33 mice and 1381 oocytes were used to explore the effects of intracellular calcium on the development and ultrastructures of oocytes subjected to 2-aminoethoxydiph...
Source: Cryobiology - December 8, 2023 Category: Biology Authors: Junping Cheng Xiaoli Wang Chan Luo Xianbao Mao Jie Qin Yan Chi Bing He Yanrong Hao Xiangli Niu Ben Huang Liling Liu Source Type: research
Effect of freeze-dried protectants on the survival rate and fermentation performance of fermented milk's directed vat set starters
Cryobiology. 2023 Dec 5:104811. doi: 10.1016/j.cryobiol.2023.104811. Online ahead of print.ABSTRACTA directed vat set (DVS) starter was proposed to improve the drawbacks of liquid starters in fermented production and enhance the survival rates of B. animalissubsp. lactis BZ11, S. thermophilus Q-1, and Lactiplantibacillus plantarum LB12. The protective agent formula was optimized using the response surface method (RSM), with the survival rate as the benchmark. The best combination of cryoprotectants was determined to be BZ11: 10 % skimmed milk powder, 3 % sodium glutamate, and 15 % trehalose; LB12: 10 % skim milk powder, 5 ...
Source: Cryobiology - December 7, 2023 Category: Biology Authors: Yue Tian Zuyan He Laping He Cuiqin Li Shunbin Qiao Han Tao Xiao Wang Xuefeng Zeng Yun Tian Source Type: research
Effect of freeze-dried protectants on the survival rate and fermentation performance of fermented milk's directed vat set starters
Cryobiology. 2023 Dec 5:104811. doi: 10.1016/j.cryobiol.2023.104811. Online ahead of print.ABSTRACTA directed vat set (DVS) starter was proposed to improve the drawbacks of liquid starters in fermented production and enhance the survival rates of B. animalissubsp. lactis BZ11, S. thermophilus Q-1, and Lactiplantibacillus plantarum LB12. The protective agent formula was optimized using the response surface method (RSM), with the survival rate as the benchmark. The best combination of cryoprotectants was determined to be BZ11: 10 % skimmed milk powder, 3 % sodium glutamate, and 15 % trehalose; LB12: 10 % skim milk powder, 5 ...
Source: Cryobiology - December 7, 2023 Category: Biology Authors: Yue Tian Zuyan He Laping He Cuiqin Li Shunbin Qiao Han Tao Xiao Wang Xuefeng Zeng Yun Tian Source Type: research
Cryogenic enrichment of Plasmodium falciparum gametocytes from spiked whole blood
Cryobiology. 2023 Nov 29:104810. doi: 10.1016/j.cryobiol.2023.104810. Online ahead of print.ABSTRACTEach individual cell type typically requires a unique set of conditions for optimal cryopreservation outcome, which relates to its specific response to cryoprotective agent (CPA) toxicity, osmotic behavior and sensitivity to ice crystallization. Cryopreservation of heterogenous cell populations is therefore exceedingly difficult as it requires separate and often conflicting conditions for each cell type. Conversely, these contrasting conditions could be utilized to favor cryogenic preference of a single cell population withi...
Source: Cryobiology - December 1, 2023 Category: Biology Authors: Jenny E Nesbitt Justyna J Jaskiewicz Hailey Bean Mehmet Toner Shannon N Tessier Rebecca D Sandlin Source Type: research
Cryogenic enrichment of Plasmodium falciparum gametocytes from spiked whole blood
Cryobiology. 2023 Nov 29:104810. doi: 10.1016/j.cryobiol.2023.104810. Online ahead of print.ABSTRACTEach individual cell type typically requires a unique set of conditions for optimal cryopreservation outcome, which relates to its specific response to cryoprotective agent (CPA) toxicity, osmotic behavior and sensitivity to ice crystallization. Cryopreservation of heterogenous cell populations is therefore exceedingly difficult as it requires separate and often conflicting conditions for each cell type. Conversely, these contrasting conditions could be utilized to favor cryogenic preference of a single cell population withi...
Source: Cryobiology - December 1, 2023 Category: Biology Authors: Jenny E Nesbitt Justyna J Jaskiewicz Hailey Bean Mehmet Toner Shannon N Tessier Rebecca D Sandlin Source Type: research
Cryogenic enrichment of Plasmodium falciparum gametocytes from spiked whole blood
Cryobiology. 2023 Nov 29:104810. doi: 10.1016/j.cryobiol.2023.104810. Online ahead of print.ABSTRACTEach individual cell type typically requires a unique set of conditions for optimal cryopreservation outcome, which relates to its specific response to cryoprotective agent (CPA) toxicity, osmotic behavior and sensitivity to ice crystallization. Cryopreservation of heterogenous cell populations is therefore exceedingly difficult as it requires separate and often conflicting conditions for each cell type. Conversely, these contrasting conditions could be utilized to favor cryogenic preference of a single cell population withi...
Source: Cryobiology - December 1, 2023 Category: Biology Authors: Jenny E Nesbitt Justyna J Jaskiewicz Hailey Bean Mehmet Toner Shannon N Tessier Rebecca D Sandlin Source Type: research
Cryogenic enrichment of Plasmodium falciparum gametocytes from spiked whole blood
Cryobiology. 2023 Nov 29:104810. doi: 10.1016/j.cryobiol.2023.104810. Online ahead of print.ABSTRACTEach individual cell type typically requires a unique set of conditions for optimal cryopreservation outcome, which relates to its specific response to cryoprotective agent (CPA) toxicity, osmotic behavior and sensitivity to ice crystallization. Cryopreservation of heterogenous cell populations is therefore exceedingly difficult as it requires separate and often conflicting conditions for each cell type. Conversely, these contrasting conditions could be utilized to favor cryogenic preference of a single cell population withi...
Source: Cryobiology - December 1, 2023 Category: Biology Authors: Jenny E Nesbitt Justyna J Jaskiewicz Hailey Bean Mehmet Toner Shannon N Tessier Rebecca D Sandlin Source Type: research
Cryogenic enrichment of Plasmodium falciparum gametocytes from spiked whole blood
Cryobiology. 2023 Nov 29:104810. doi: 10.1016/j.cryobiol.2023.104810. Online ahead of print.ABSTRACTEach individual cell type typically requires a unique set of conditions for optimal cryopreservation outcome, which relates to its specific response to cryoprotective agent (CPA) toxicity, osmotic behavior and sensitivity to ice crystallization. Cryopreservation of heterogenous cell populations is therefore exceedingly difficult as it requires separate and often conflicting conditions for each cell type. Conversely, these contrasting conditions could be utilized to favor cryogenic preference of a single cell population withi...
Source: Cryobiology - December 1, 2023 Category: Biology Authors: Jenny E Nesbitt Justyna J Jaskiewicz Hailey Bean Mehmet Toner Shannon N Tessier Rebecca D Sandlin Source Type: research
Is post-hypertonic lysis of human red blood cells caused by excessive cell volume regulation?
In this study, the hypertonicity-related behavior of human RBCs is revisited within the framework of modern cell physiology, considering current knowledge on membrane ion transport mechanisms - an account still missing. It is recognized here that the hypertonic behavior of human RBCs is consistent with the acute regulatory volume increase (RVI) response - a healthy physiological reaction initiated by cells to regulate their volume by salt accumulation. It is shown by reviewing the published studies that human RBCs can increase cation conductance considerably by activating cell volume-regulated ion transport pathways inacti...
Source: Cryobiology - November 20, 2023 Category: Biology Authors: Ivan Klbik Source Type: research
Is post-hypertonic lysis of human red blood cells caused by excessive cell volume regulation?
In this study, the hypertonicity-related behavior of human RBCs is revisited within the framework of modern cell physiology, considering current knowledge on membrane ion transport mechanisms - an account still missing. It is recognized here that the hypertonic behavior of human RBCs is consistent with the acute regulatory volume increase (RVI) response - a healthy physiological reaction initiated by cells to regulate their volume by salt accumulation. It is shown by reviewing the published studies that human RBCs can increase cation conductance considerably by activating cell volume-regulated ion transport pathways inacti...
Source: Cryobiology - November 20, 2023 Category: Biology Authors: Ivan Klbik Source Type: research
Is post-hypertonic lysis of human red blood cells caused by excessive cell volume regulation?
In this study, the hypertonicity-related behavior of human RBCs is revisited within the framework of modern cell physiology, considering current knowledge on membrane ion transport mechanisms - an account still missing. It is recognized here that the hypertonic behavior of human RBCs is consistent with the acute regulatory volume increase (RVI) response - a healthy physiological reaction initiated by cells to regulate their volume by salt accumulation. It is shown by reviewing the published studies that human RBCs can increase cation conductance considerably by activating cell volume-regulated ion transport pathways inacti...
Source: Cryobiology - November 20, 2023 Category: Biology Authors: Ivan Klbik Source Type: research
