Use of fine capillaries for cryopreservation of Caenorhabditis elegans by vitrification
Cryobiology. 2023 Sep 9;113:104585. doi: 10.1016/j.cryobiol.2023.104585. Online ahead of print.ABSTRACTCaenorhabditis elegans is an exceptional model organism. More than twenty thousand different strains have been developed, increasing knowledge on countless topics. However, the traditional method to cryopreserve this nematode, based on slow freezing, usually reaches recovery rates of around 35% for the L1 and L2 larval stages. Here, we propose two alternative methods to cryopreserve this nematode based on vitrification that are applicable in common laboratories and allow the selective individual cryopreservation of this o...
Source: Cryobiology - September 10, 2023 Category: Biology Authors: Daniel Barranco Ver ónica Cabo-Ruiz Ram ón Risco Source Type: research

Use of fine capillaries for cryopreservation of Caenorhabditis elegans by vitrification
Cryobiology. 2023 Sep 9;113:104585. doi: 10.1016/j.cryobiol.2023.104585. Online ahead of print.ABSTRACTCaenorhabditis elegans is an exceptional model organism. More than twenty thousand different strains have been developed, increasing knowledge on countless topics. However, the traditional method to cryopreserve this nematode, based on slow freezing, usually reaches recovery rates of around 35% for the L1 and L2 larval stages. Here, we propose two alternative methods to cryopreserve this nematode based on vitrification that are applicable in common laboratories and allow the selective individual cryopreservation of this o...
Source: Cryobiology - September 10, 2023 Category: Biology Authors: Daniel Barranco Ver ónica Cabo-Ruiz Ram ón Risco Source Type: research

Use of fine capillaries for cryopreservation of Caenorhabditis elegans by vitrification
Cryobiology. 2023 Sep 8:104585. doi: 10.1016/j.cryobiol.2023.104585. Online ahead of print.ABSTRACTCaenorhabditis elegans is an exceptional model organism. More than twenty thousand different strains have been developed, increasing knowledge on countless topics. However, the traditional method to cryopreserve this nematode, based on slow freezing, usually reaches recovery rates of around 35% for the L1 and L2 larval stages. Here, we propose two alternative methods to cryopreserve this nematode based on vitrification that are applicable in common laboratories and allow the selective individual cryopreservation of this organ...
Source: Cryobiology - September 10, 2023 Category: Biology Authors: Daniel Barranco Ver ónica Cabo-Ruiz Ram ón Risco Source Type: research

Use of fine capillaries for cryopreservation of Caenorhabditis elegans by vitrification
Cryobiology. 2023 Sep 8:104585. doi: 10.1016/j.cryobiol.2023.104585. Online ahead of print.ABSTRACTCaenorhabditis elegans is an exceptional model organism. More than twenty thousand different strains have been developed, increasing knowledge on countless topics. However, the traditional method to cryopreserve this nematode, based on slow freezing, usually reaches recovery rates of around 35% for the L1 and L2 larval stages. Here, we propose two alternative methods to cryopreserve this nematode based on vitrification that are applicable in common laboratories and allow the selective individual cryopreservation of this organ...
Source: Cryobiology - September 10, 2023 Category: Biology Authors: Daniel Barranco Ver ónica Cabo-Ruiz Ram ón Risco Source Type: research

Use of fine capillaries for cryopreservation of Caenorhabditis elegans by vitrification
Cryobiology. 2023 Sep 8:104585. doi: 10.1016/j.cryobiol.2023.104585. Online ahead of print.ABSTRACTCaenorhabditis elegans is an exceptional model organism. More than twenty thousand different strains have been developed, increasing knowledge on countless topics. However, the traditional method to cryopreserve this nematode, based on slow freezing, usually reaches recovery rates of around 35% for the L1 and L2 larval stages. Here, we propose two alternative methods to cryopreserve this nematode based on vitrification that are applicable in common laboratories and allow the selective individual cryopreservation of this organ...
Source: Cryobiology - September 10, 2023 Category: Biology Authors: Daniel Barranco Ver ónica Cabo-Ruiz Ram ón Risco Source Type: research

Droplet freezing assays using a nanoliter osmometer
Cryobiology. 2023 Sep 7:104584. doi: 10.1016/j.cryobiol.2023.104584. Online ahead of print.ABSTRACTThe ability to accurately record the temperature at which ice nucleation occurs is critical for studying biological ice nucleators. Several instruments have been designed and custom built to make such measurements, but they are not yet on the market. Here we reproducibly measure ice nucleation temperatures down close to the homogeneous nucleation temperature of -38 °C with a commercially available nanoliter osmometer, which we routinely use to assay the thermal hysteresis activity of antifreeze proteins. This instrument has ...
Source: Cryobiology - September 9, 2023 Category: Biology Authors: Jocelyn C Lee Thomas Hansen Peter L Davies Source Type: research

Meta-analysis of the Boyle van 't Hoff relation: Turgor and leak models explain non-ideal volume equilibrium
This study evaluates the generality of this and other long-standing assumptions about cell responses to anisotonic conditions. We present alternative models that account for osmoregulation including mechanical resistance to volumetric expansion (the turgor model) and ion-osmolyte leakage (the leak model). To evaluate the generality of the BvH relation and determine the suitability of alternative models, we performed a comprehensive survey of the literature and a careful analysis of the resulting data, and then we used these data to compare among models. We identified 137 articles published from 1964 to 2019 spanning 14 ani...
Source: Cryobiology - September 3, 2023 Category: Biology Authors: Dominic J Olver James D Benson Source Type: research

Meta-analysis of the Boyle van 't Hoff relation: Turgor and leak models explain non-ideal volume equilibrium
This study evaluates the generality of this and other long-standing assumptions about cell responses to anisotonic conditions. We present alternative models that account for osmoregulation including mechanical resistance to volumetric expansion (the turgor model) and ion-osmolyte leakage (the leak model). To evaluate the generality of the BvH relation and determine the suitability of alternative models, we performed a comprehensive survey of the literature and a careful analysis of the resulting data, and then we used these data to compare among models. We identified 137 articles published from 1964 to 2019 spanning 14 ani...
Source: Cryobiology - September 3, 2023 Category: Biology Authors: Dominic J Olver James D Benson Source Type: research

Meta-analysis of the Boyle van 't Hoff relation: Turgor and leak models explain non-ideal volume equilibrium
This study evaluates the generality of this and other long-standing assumptions about cell responses to anisotonic conditions. We present alternative models that account for osmoregulation including mechanical resistance to volumetric expansion (the turgor model) and ion-osmolyte leakage (the leak model). To evaluate the generality of the BvH relation and determine the suitability of alternative models, we performed a comprehensive survey of the literature and a careful analysis of the resulting data, and then we used these data to compare among models. We identified 137 articles published from 1964 to 2019 spanning 14 ani...
Source: Cryobiology - September 3, 2023 Category: Biology Authors: Dominic J Olver James D Benson Source Type: research

Meta-analysis of the Boyle van 't Hoff relation: Turgor and leak models explain non-ideal volume equilibrium
This study evaluates the generality of this and other long-standing assumptions about cell responses to anisotonic conditions. We present alternative models that account for osmoregulation including mechanical resistance to volumetric expansion (the turgor model) and ion-osmolyte leakage (the leak model). To evaluate the generality of the BvH relation and determine the suitability of alternative models, we performed a comprehensive survey of the literature and a careful analysis of the resulting data, and then we used these data to compare among models. We identified 137 articles published from 1964 to 2019 spanning 14 ani...
Source: Cryobiology - September 3, 2023 Category: Biology Authors: Dominic J Olver James D Benson Source Type: research

Meta-analysis of the Boyle van 't Hoff relation: Turgor and leak models explain non-ideal volume equilibrium
This study evaluates the generality of this and other long-standing assumptions about cell responses to anisotonic conditions. We present alternative models that account for osmoregulation including mechanical resistance to volumetric expansion (the turgor model) and ion-osmolyte leakage (the leak model). To evaluate the generality of the BvH relation and determine the suitability of alternative models, we performed a comprehensive survey of the literature and a careful analysis of the resulting data, and then we used these data to compare among models. We identified 137 articles published from 1964 to 2019 spanning 14 ani...
Source: Cryobiology - September 3, 2023 Category: Biology Authors: Dominic J Olver James D Benson Source Type: research

Successful recovery of motile and viable boar sperm after vitrification with different methods (pearls and mini straws) using sucrose as a cryoprotectant
In conclusion, the pearl method was the most suitable of the vitrification techniques for use with boar sperm.PMID:37659575 | DOI:10.1016/j.cryobiol.2023.104583 (Source: Cryobiology)
Source: Cryobiology - September 2, 2023 Category: Biology Authors: Mar ía Serrano Albal Marie Claire Aquilina Louisa J Zak Peter J Ellis Darren K Griffin Source Type: research

Successful recovery of motile and viable boar sperm after vitrification with different methods (pearls and mini straws) using sucrose as a cryoprotectant
In conclusion, the pearl method was the most suitable of the vitrification techniques for use with boar sperm.PMID:37659575 | DOI:10.1016/j.cryobiol.2023.104583 (Source: Cryobiology)
Source: Cryobiology - September 2, 2023 Category: Biology Authors: Mar ía Serrano Albal Marie Claire Aquilina Louisa J Zak Peter J Ellis Darren K Griffin Source Type: research

Exploring the feasibility of cryopreserving larvae of the common cockle (Cerastoderma edule) for hatchery production
Cryobiology. 2023 Aug 30:104582. doi: 10.1016/j.cryobiol.2023.104582. Online ahead of print.ABSTRACTThe decline of natural populations of the common cockle (Cerastoderma edule) through the European coast is posing a threat to local small-scale fisheries. These declines are primarily attributed to the prevalence of several pathogens and the disseminated neoplasia in cockle populations. The institution of a biobank of cryopreserved larvae could enhance hatchery production and help the restocking. The present work aimed at the development of a cryopreservation protocol for larvae of the common cockle using the mollusk cryopre...
Source: Cryobiology - September 1, 2023 Category: Biology Authors: P Heres J Troncoso E Paredes Source Type: research

Exploring the feasibility of cryopreserving larvae of the common cockle (Cerastoderma edule) for hatchery production
Cryobiology. 2023 Aug 30:104582. doi: 10.1016/j.cryobiol.2023.104582. Online ahead of print.ABSTRACTThe decline of natural populations of the common cockle (Cerastoderma edule) through the European coast is posing a threat to local small-scale fisheries. These declines are primarily attributed to the prevalence of several pathogens and the disseminated neoplasia in cockle populations. The institution of a biobank of cryopreserved larvae could enhance hatchery production and help the restocking. The present work aimed at the development of a cryopreservation protocol for larvae of the common cockle using the mollusk cryopre...
Source: Cryobiology - September 1, 2023 Category: Biology Authors: P Heres J Troncoso E Paredes Source Type: research